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WO2019017691A3 - Method of differentiation of human induced pluripotent stem cell to dermal papilla precursor cell and use thereof - Google Patents

Method of differentiation of human induced pluripotent stem cell to dermal papilla precursor cell and use thereof Download PDF

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Publication number
WO2019017691A3
WO2019017691A3 PCT/KR2018/008123 KR2018008123W WO2019017691A3 WO 2019017691 A3 WO2019017691 A3 WO 2019017691A3 KR 2018008123 W KR2018008123 W KR 2018008123W WO 2019017691 A3 WO2019017691 A3 WO 2019017691A3
Authority
WO
WIPO (PCT)
Prior art keywords
dermal papilla
precursor cell
differentiation
human
papilla precursor
Prior art date
Application number
PCT/KR2018/008123
Other languages
French (fr)
Korean (ko)
Other versions
WO2019017691A9 (en
WO2019017691A2 (en
Inventor
권오상
강보미
김진용
권유욱
Original Assignee
서울대학교 산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 서울대학교 산학협력단 filed Critical 서울대학교 산학협력단
Priority to US16/632,472 priority Critical patent/US11629331B2/en
Priority claimed from KR1020180083386A external-priority patent/KR102122039B1/en
Publication of WO2019017691A2 publication Critical patent/WO2019017691A2/en
Publication of WO2019017691A3 publication Critical patent/WO2019017691A3/en
Publication of WO2019017691A9 publication Critical patent/WO2019017691A9/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0627—Hair cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Dermatology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The present invention relates to a medium composition for differentiation of a human induced pluripotent stem cell to a dermal papilla precursor cell, a differentiation method, and a use for inducing hair follicle neogenesis using the differentiated dermal papilla precursor cell. The present inventors have developed a method for differentiating a human induced pluripotent stem cell into a dermal papilla precursor cell having hair follicle forming ability and a composition of a dermal papilla precursor cell specific differentiation medium for the above differentiation, and have effectively induced hair follicle neogenesis consisting only of human cells without conventional mouse-human hybrid hair follicles by using the human induced dermal papilla precursor cell and a human induced epidermal precursor cell obtained through the differentiation method. Human hair follicle tissue produced by applying the technique for differentiating the dermal papilla precursor cell according to the present invention is expected to be useful as a therapeutic method for patients suffering from hair loss by overcoming the limitations of hair loss treatments.
PCT/KR2018/008123 2017-07-19 2018-07-18 Method of differentiation of human induced pluripotent stem cell to dermal papilla precursor cell and use thereof WO2019017691A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US16/632,472 US11629331B2 (en) 2017-07-19 2018-07-18 Method of differentiation of human induced pluripotent stem cell to dermal papilla precursor cell and use thereof

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20170091727 2017-07-19
KR10-2017-0091727 2017-07-19
KR10-2018-0083386 2018-07-18
KR1020180083386A KR102122039B1 (en) 2017-07-19 2018-07-18 Method for differentiation of dermal papilla precursor cells from human pluripotent stem cells and uses thereof

Publications (3)

Publication Number Publication Date
WO2019017691A2 WO2019017691A2 (en) 2019-01-24
WO2019017691A3 true WO2019017691A3 (en) 2019-03-28
WO2019017691A9 WO2019017691A9 (en) 2019-05-09

Family

ID=65015799

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2018/008123 WO2019017691A2 (en) 2017-07-19 2018-07-18 Method of differentiation of human induced pluripotent stem cell to dermal papilla precursor cell and use thereof

Country Status (1)

Country Link
WO (1) WO2019017691A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4022037A1 (en) * 2019-07-10 2022-07-06 Helmuth Heinrich Kunz Methods for deriving autologous and hypoimmunogenic hair follicle containing sheets in vitro
CN115418341B (en) * 2022-09-22 2024-03-15 北京雍禾医疗投资管理有限公司 Method for transdifferentiation of fibroblast to hair papilla cell and application thereof
KR102543431B1 (en) * 2022-10-05 2023-06-15 주식회사 프롬바이오 Compostion for inducing differentiation of adipose mesenchymal stem cells to dermal papilla cells and the composition for differentiating using the composition

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016114723A1 (en) * 2015-01-16 2016-07-21 Agency For Science, Technology And Research Differentiation of macrophages from pluripotent stem cells
KR20160145170A (en) * 2014-04-21 2016-12-19 카오카부시키가이샤 Method of producing skin-derived precursor cells
WO2017070506A1 (en) * 2015-10-21 2017-04-27 Indiana University Research And Technology Corporation Derivation of human skin organoids from pluripotent stem cells

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20160145170A (en) * 2014-04-21 2016-12-19 카오카부시키가이샤 Method of producing skin-derived precursor cells
WO2016114723A1 (en) * 2015-01-16 2016-07-21 Agency For Science, Technology And Research Differentiation of macrophages from pluripotent stem cells
WO2017070506A1 (en) * 2015-10-21 2017-04-27 Indiana University Research And Technology Corporation Derivation of human skin organoids from pluripotent stem cells

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
GNEDEVA, KSENIA ET AL.: "Derivation of Hair-inducing Cell from Human Pluripotent Stem Cells", PLOS ONE, vol. 10, no. 1, 2015, pages 1 - 14, XP055584890 *
IN AMATSU, MUTSUMI ET AL.: "Embryonic Dermal Condensation and Adult Dermal Papilla Induce Hair Follicles in Adult Glabrous Epidermis through Different Mechanisms", DEVELOPMENT, GROWTH & DIFFERENTIATION, vol. 48, 2006, pages 73 - 86, XP055584889 *
KIM, J. ET AL.: "Human Hair Follicle Regeneration with Trichogenic Human Dermal Papilla Precursor Cells Derived from Induced Pluripotent Stem Cells", 76TH ANNUAL MEETING PORTLAND OREGON OF SOCIETY FOR INVESTIGATIVE DERMATOLOGY, 27 April 2017 (2017-04-27) *
VERAITCH, OPHELIA ET AL.: "Induction of Hair Follicle Dermal Papilla Cell Properties in Human Induced Pluripotent Stem Cell -derived Multipotent LNGFR(+)THY-1(+) Mesenchymal Cells", SCIENTIFIC REPORTS, vol. 7, no. 42777, 21 February 2017 (2017-02-21), pages 1 - 13, XP055446550 *

Also Published As

Publication number Publication date
WO2019017691A9 (en) 2019-05-09
WO2019017691A2 (en) 2019-01-24

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