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WO2018192036A1 - Solid phase carrier and test kit used for enzyme-linked immunosorbent assay - Google Patents

Solid phase carrier and test kit used for enzyme-linked immunosorbent assay Download PDF

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Publication number
WO2018192036A1
WO2018192036A1 PCT/CN2017/084311 CN2017084311W WO2018192036A1 WO 2018192036 A1 WO2018192036 A1 WO 2018192036A1 CN 2017084311 W CN2017084311 W CN 2017084311W WO 2018192036 A1 WO2018192036 A1 WO 2018192036A1
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WO
WIPO (PCT)
Prior art keywords
cup
enzyme
solid phase
linked immunosorbent
immunosorbent assay
Prior art date
Application number
PCT/CN2017/084311
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French (fr)
Chinese (zh)
Inventor
董方
Original Assignee
董方
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Publication date
Application filed by 董方 filed Critical 董方
Priority to JP2020600025U priority Critical patent/JP3225987U/en
Publication of WO2018192036A1 publication Critical patent/WO2018192036A1/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic

Definitions

  • the invention relates to the field of immunoassay technology, in particular to a non-negative pressure solid phase carrier and a kit for enzyme-linked immunosorbent assay.
  • ELISA Enzyme-linked immunosorbent assay
  • the inspection automation requires that the cellulose film of the solid phase material can be adapted to the various requirements of addition, attraction, and transfer in the machine, which requires a housing for fixing the cellulose membrane.
  • the back surface of the cellulose film is often provided with a water absorbing material as a liner, and the cellulose film, the shell and the liner form various solid phases.
  • Carrier In the prior art, the housing of the solid phase carrier is designed to be cup-shaped to improve the ease of handling as a medical waste.
  • the present invention provides a solid phase carrier and a kit for enzyme-linked immunosorbent assay, which can eliminate the gas spring effect and the detection result is more accurate.
  • the present invention adopts the technical solution as follows:
  • a solid phase carrier for enzyme-linked immunosorbent assay comprising a housing and a cellulose membrane and a liner disposed in the housing from top to bottom, the upper portion of the housing being open at least one isobaric a pore, and the isopipe is not lower than the cellulose film, that is, the isopipe is located above or at the same level as the cellulose film.
  • the isopipe is preferably located above the cellulose film.
  • the housing comprises a cup-shaped cup body, and the isostatic hole is provided on the cup body at a position close to the cup mouth.
  • the inner wall of the cup is provided with a rib for contracting the pad.
  • the rib is annular.
  • the housing further includes a cup cover disposed on a cup mouth of the cup body, the cup cover having a funnel shape, the cup cover having an upper surface for draining a liquid, the upper surface being Spiral surface.
  • the cup cover is provided with a ring of card slots for engaging with the cup wall of the cup body.
  • cup body and/or the cup cover have a rectangular or rounded rectangular cross section perpendicular to the center line of the cup body.
  • the housing further includes an incubating cup attached to one side of the cup, the cup and the incubating cup being integrally formed.
  • the cup and/or the outer wall of the incubator cup is provided with at least one recess for positioning during loading.
  • a kit for enzyme-linked immunosorbent assay comprising the solid phase support for enzyme-linked immunosorbent assay.
  • An isostatic hole is formed in the casing, and the isostatic hole is not lower than the cellulose film, thereby eliminating the gas spring effect generated when the liquid is injected, ensuring the consistency of the inflow velocity when the whole process is added, so that the detection result is more Accurate, especially for elongated solid support;
  • the rib is provided on the inner wall of the cup, so that the pad shrinks at the rib to form a shrinkage port to prevent backflow of the liquid and improve the accuracy of the detection value;
  • the upper surface of the lid is a spiral curved surface, and has a guiding drainage effect on the liquid flowing to the surface of the cellulose film, so that the liquid flow rate to the cellulose film is constant, and the liquid and the cellulose film surface have good affinity;
  • the card slot on the cup cover is arranged around a circle, slotted on all sides, no directional, convenient assembly of the cup cover and the cup body;
  • Figure 1 is a cross-sectional view showing the internal structure of a solid phase carrier of the present invention
  • Figure 2 is a side view of a solid phase carrier of the present invention
  • Figure 3 is a plan view of a solid phase carrier of the present invention.
  • Figure 4 is a bottom plan view of a solid phase carrier of the invention.
  • FIG. 1-4 A solid phase carrier for enzyme-linked immunosorbent assay is shown, which is a component of a kit for enzyme-linked immunosorbent assays, which can be applied to reagents for enzyme-linked immunosorbent assays In the box.
  • the solid phase carrier includes a housing and a cellulose film 3 and a liner 4 disposed in the housing from top to bottom. (generally composed of a water absorbing material), at least one isopiped 11 is formed through the upper portion of the casing, and the isopipe 11 is not lower than the cellulose film 3, preferably located at the cellulose film 3 Above.
  • the casing is composed of a cup body 1, a cup cover 2, and an incubating cup 5, which will be described in detail below.
  • the cup 1 has a cup shape.
  • the isopipe 11 is provided on the cup 1 near the mouth of the cup, and in addition, the isopipe 11 can be provided on the lid 2 on.
  • the number of isopipes 11 is at least one, and is specifically formed on a side wall of the cup 1 opposite to the incubating cup 5.
  • the isopipe 11 Located above the cellulose film 3, as shown in Figure 1. Isobaric hole 11 allows cup 1 The inside air flows out, eliminating the gas spring effect generated when the liquid is injected, ensuring the consistency of the inflow velocity when the whole process is added, so that the detection result is more accurate, especially for the elongated solid phase carrier.
  • the inner wall of the cup 1 is provided with a rib 12 for shrinking the pad 4, and the rib 12 preferably surrounds the cup 1 A closed loop of the longitudinal centerline one turn.
  • the rib 12 is preferably disposed at a position of the cup 1 near the cup opening and below the isopiped 11. According to the capillary tension action and the valve principle, a rib is provided on the inner wall of the cup body 12 When the water absorbing pad 4 is embedded, the formed cuff suppresses the liquid that has been sucked into the inner liner 4 of the cup body 1 to flow upward, and the cellulose film 3 is secured.
  • the membrane surface is in a normal wet state after passing through the liquid, and the accuracy of the detection value is improved. This can be avoided: the existing solid phase carrier aspiration pad 4 is retrograde and then reversed to cause the cellulose film 3 There is liquid residue on the film surface, and the detection result is wrong.
  • the air in the cup 1 flows up and down, and a small hole 13 is formed in the bottom wall of the cup body 1.
  • the bottom of the cup 1 is provided with a projection 14 extending toward the inside of the cup 1.
  • Raised 14 It is preferably disposed at an intermediate position at the bottom of the cup 1.
  • the section of the cup 1 perpendicular to its longitudinal centerline has a rectangular or rounded rectangle, that is, the cup 1 It is roughly cuboid or rectangular in shape after rounding. This allows the cup 1 to have a space in which the pad 4 is allowed to swell and absorb, and the gas spring effect is relatively small.
  • the cup lid 2 is perpendicular to the cup 1
  • the longitudinal centerline has a rectangular or rounded rectangle.
  • the lid 2 is provided on the cup mouth of the cup body 1.
  • Cup lid 2 is funnel shaped, cup lid 2
  • the spiral curved surface 21 can flow toward the cellulose film 3 compared to the bevel in the prior art.
  • the liquid on the surface is guided to conduct a flow so that the flow rate of the liquid to the cellulose film 3 is constant, and the liquid and the surface of the cellulose film 3 have a good affinity.
  • the lid 2 is located above the cellulose film 3 and the liner 4, against the cellulose film 3 and the liner 4 Fix it to ensure that the two are in close contact.
  • the lid 2 accommodates a certain amount of reagent input and ensures that all reagents pass through the center.
  • the cup cover 2 is provided with a ring slot 22 for engaging with the cup wall of the cup 1 , and the card slot 22 Surrounded by a circle, slotted on all sides, non-directional, convenient assembly of the lid 2 and the cup 1.
  • the incubating cup 5 is attached to one side of the cup body 1, and the cup body 1 and the incubating cup 5 are integrally formed. Incubation cup 5 and isobaric 11 Located on opposite sides of the cup 1 respectively.
  • the outer wall of the cup 1 and/or the incubating cup 5 is provided with at least one recess 51 for positioning during loading.
  • Groove 51 Preferably, they are arranged in pairs and are respectively located at the junction of the cup 1 and the incubator cup 5.
  • the outer wall of the cup body 1 and the incubating cup 5 is provided with a groove 51 for positioning for convenient loading.

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A solid phase carrier and a test kit used for enzyme-linked immunosorbent assay, which may eliminate a gas-spring effect and render an accurate test result. The solid phase carrier for the enzyme-linked immunosorbent assay comprises a shell, as well as a cellulose membrane and a liner which are arranged in the shell and are arranged sequentially from top to bottom; at least one equal pressure through-hole is opened on the top of the shell, and the equal pressure holes are not lower than the cellulosic membrane. The shell comprises a cup-shaped cup body, and the equal pressure holes are provided on the cup body and at a position close to the cup opening.

Description

一种用于酶联免疫吸附测定的固相载体及试剂盒Solid phase carrier and kit for enzyme-linked immunosorbent assay
技术领域Technical field
本发明涉及免疫检测技术领域，特别涉及一种用于酶联免疫吸附测定的无负压的固相载体及试剂盒。 The invention relates to the field of immunoassay technology, in particular to a non-negative pressure solid phase carrier and a kit for enzyme-linked immunosorbent assay.
背景技术 Background technique
以 ELISA （酶联免疫吸附测定）为基本原理的免疫检测技术中，单单靠加样杯杯壁作为固相已经不能满足高灵敏度检测的需要。为求得更大的固相表面积，采用多孔性的纤维素膜作为固相材料已在许多试剂盒中得到应用。 ELISA (Enzyme-linked immunosorbent assay) In the basic principle of immunoassay technology, simply using the cup wall as a solid phase can not meet the needs of high-sensitivity detection. In order to obtain a larger solid surface area, the use of a porous cellulose film as a solid phase material has been used in many kits.
检测自动化要求固相材料的纤维素膜片装机后能够适应机内各项添加、吸引、传送等动作要求，这就需要有一个固定纤维素膜片的壳体。为保证对纤维素膜加样后通过纤维素膜的液流速度均匀，纤维素膜的背面往往加有吸水材料作为衬垫，纤维素膜片、壳体及衬垫形成各种样式的固相载体。现有技术中有将固相载体的壳体设计成杯状，以提高作为医疗废弃物时处理的简便性。 The inspection automation requires that the cellulose film of the solid phase material can be adapted to the various requirements of addition, attraction, and transfer in the machine, which requires a housing for fixing the cellulose membrane. In order to ensure uniform flow rate of the cellulose film after loading the cellulose film, the back surface of the cellulose film is often provided with a water absorbing material as a liner, and the cellulose film, the shell and the liner form various solid phases. Carrier. In the prior art, the housing of the solid phase carrier is designed to be cup-shaped to improve the ease of handling as a medical waste.
然而，在现有技术至少存在以下问题： However, at least the following problems exist in the prior art:
壳体中仅吸水衬垫下方有等大气压孔，当吸入液体达到一定量时，壳体内部与大气隔断，后续进入的液体将因气弹簧效应而潴留，影响检测结果的准确性。尤其在细长形的固相载体中，气弹簧效应会更为显著。 In the casing, only the atmospheric pressure hole is arranged under the water absorption pad. When the suction liquid reaches a certain amount, the inside of the casing is separated from the atmosphere, and the subsequently entered liquid will be retained due to the gas spring effect, which affects the accuracy of the detection result. Especially in the elongated solid phase carrier, the gas spring effect is more pronounced.
发明内容 Summary of the invention
针对上述问题，本发明提供一种用于酶联免疫吸附测定的固相载体及试剂盒，其可消除气弹簧效应，检测结果较准确。 In view of the above problems, the present invention provides a solid phase carrier and a kit for enzyme-linked immunosorbent assay, which can eliminate the gas spring effect and the detection result is more accurate.
为达到上述目的，本发明采用技术方案为： In order to achieve the above object, the present invention adopts the technical solution as follows:
一种用于酶联免疫吸附测定的固相载体，包括壳体以及自上至下依次设置在所述壳体内的纤维素膜和衬垫，所述壳体的上部贯通开设有至少一个等压孔，且所述等压孔不低于所述纤维素膜，也就是说等压孔位于纤维素膜的上方或与纤维素膜持平。等压孔优选为位于纤维素膜的上方。 A solid phase carrier for enzyme-linked immunosorbent assay comprising a housing and a cellulose membrane and a liner disposed in the housing from top to bottom, the upper portion of the housing being open at least one isobaric a pore, and the isopipe is not lower than the cellulose film, that is, the isopipe is located above or at the same level as the cellulose film. The isopipe is preferably located above the cellulose film.
优选地，所述壳体包括呈杯状的杯体，所述等压孔设于所述杯体上的靠近杯口的位置。 Preferably, the housing comprises a cup-shaped cup body, and the isostatic hole is provided on the cup body at a position close to the cup mouth.
更优选地，所述杯体内壁设有用于收缩所述衬垫的凸棱。 More preferably, the inner wall of the cup is provided with a rib for contracting the pad.
进一步地，所述凸棱为环形。 Further, the rib is annular.
优选地，所述壳体还包括设于所述杯体的杯口上的杯盖，所述杯盖呈漏斗形，所述杯盖具有用于对液体进行引流的上表面，所述上表面为螺旋曲面。 Preferably, the housing further includes a cup cover disposed on a cup mouth of the cup body, the cup cover having a funnel shape, the cup cover having an upper surface for draining a liquid, the upper surface being Spiral surface.
更优选地，所述杯盖上开设有用于与所述杯体的杯壁相配合卡接的一圈卡槽。 More preferably, the cup cover is provided with a ring of card slots for engaging with the cup wall of the cup body.
进一步地，所述杯体和 / 或所述杯盖的垂直于杯体中心线的截面为矩形或圆角矩形。 Further, the cup body and/or the cup cover have a rectangular or rounded rectangular cross section perpendicular to the center line of the cup body.
优选地，所述壳体还包括连接在所述杯体一侧的孵育杯，所述杯体和所述孵育杯一体成形。 Preferably, the housing further includes an incubating cup attached to one side of the cup, the cup and the incubating cup being integrally formed.
更优选地，所述杯体和 / 或所述孵育杯的外壁上设有至少一个用于在加载时进行定位的凹槽。 More preferably, the cup and/or the outer wall of the incubator cup is provided with at least one recess for positioning during loading.
本发明还采用如下技术方案： The invention also adopts the following technical solutions:
一种用于酶联免疫吸附测定的试剂盒，包括所述的用于酶联免疫吸附测定的固相载体。 A kit for enzyme-linked immunosorbent assay comprising the solid phase support for enzyme-linked immunosorbent assay.
本发明采用以上方案，相比现有技术具有如下优点： The present invention adopts the above scheme and has the following advantages compared with the prior art:
1 、在壳体上开设等压孔，且等压孔不低于纤维素膜，消除了液体注入时产生的气弹簧效应，确保检测全程加液时的流入速度的一致性，使得检测结果更为准确，尤其是对于细长形的固相载体； 1 An isostatic hole is formed in the casing, and the isostatic hole is not lower than the cellulose film, thereby eliminating the gas spring effect generated when the liquid is injected, ensuring the consistency of the inflow velocity when the whole process is added, so that the detection result is more Accurate, especially for elongated solid support;
2 、杯体内壁上设置凸棱，使衬垫在凸棱处收缩，形成一个收缩口，防止液体逆流，提高检测值的准确性； 2, the rib is provided on the inner wall of the cup, so that the pad shrinks at the rib to form a shrinkage port to prevent backflow of the liquid and improve the accuracy of the detection value;
3 、杯盖的上表面为螺旋曲面，对流向纤维素膜表面的液体具有导向引流作用，使流向纤维素膜的液体流速一定，液体和纤维素膜表面具有较好的亲和性； 3 The upper surface of the lid is a spiral curved surface, and has a guiding drainage effect on the liquid flowing to the surface of the cellulose film, so that the liquid flow rate to the cellulose film is constant, and the liquid and the cellulose film surface have good affinity;
4 、杯盖上的卡槽环绕一圈设置，四面开槽，无方向性，方便杯盖与杯体的装配； 4, the card slot on the cup cover is arranged around a circle, slotted on all sides, no directional, convenient assembly of the cup cover and the cup body;
5 、将原来需要别加载的固相载体和孵育杯两者一体化，简化了工序提高了自动化程度；杯体和孵育杯的外壁上开设有定位用的凹槽，方便加载。 5 The integration of the solid phase carrier and the incubating cup which are originally required to be loaded is simplified, the process is simplified and the degree of automation is improved; the outer wall of the cup body and the incubating cup is provided with a groove for positioning for convenient loading.
附图说明 DRAWINGS
为了更清楚地说明本发明的技术方案，下面将对实施例描述中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图仅仅是本发明的一些实施例，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据这些附图获得其他的附图。 In order to more clearly illustrate the technical solutions of the present invention, the drawings used in the description of the embodiments will be briefly described below. It is obvious that the drawings in the following description are only some embodiments of the present invention. For the ordinary technicians, other drawings can be obtained based on these drawings without any creative work.
附图 1 为示出了本发明的一种固相载体内部结构的剖视图； Figure 1 is a cross-sectional view showing the internal structure of a solid phase carrier of the present invention;
附图 2 为本发明的一种固相载体的侧视图； Figure 2 is a side view of a solid phase carrier of the present invention;
附图 3 为本发明的一种固相载体的俯视图； Figure 3 is a plan view of a solid phase carrier of the present invention;
附图 4 为发明的一种固相载体的仰视图。 Figure 4 is a bottom plan view of a solid phase carrier of the invention.
以上附图中， In the above figures,
1 、杯体； 11 、等压孔； 12 、凸棱； 13 、小孔； 14 、凸起； 1 , cup body; 11, equal pressure hole; 12, rib; 13 , small hole; 14 , convex;
2 、杯盖； 21 、螺旋曲面； 22 、卡槽； 2, cup cover; 21, spiral curved surface; 22, card slot;
3 、纤维素膜； 3, cellulose film;
4 、衬垫； 4, pad;
5 、孵育杯； 51 、凹槽。 5, incubate cup; 51, groove.
具体实施方式 detailed description
下面结合附图对本发明的较佳实施例进行详细阐述，以使本发明的优点和特征能更易于被本领域的技术人员理解。在此需要说明的是，对于这些实施方式的说明用于帮助理解本发明，但并不构成对本发明的限定。此外，下面所描述的本发明各个实施方式中所涉及到的技术特征只要彼此之间未构成冲突就可以互相结合。 The preferred embodiments of the present invention are described in detail below with reference to the accompanying drawings, in which the advantages and features of the invention can be more readily understood by those skilled in the art. It is to be noted that the description of the embodiments is intended to facilitate the understanding of the invention, but is not intended to limit the invention. Further, the technical features involved in the various embodiments of the present invention described below may be combined with each other as long as they do not constitute a conflict with each other.
本发明中述及的上、下等方位词是根据本领域技术人员的惯常观察视角以及为了叙述方便而定义的，不限定具体的方向。如，分别对应于附图 1 中纸面的上侧、下侧。 The above-mentioned upper and lower orientation words are defined according to the conventional observation angles of those skilled in the art and are convenient for the description, and do not limit the specific directions. For example, they correspond to the upper side and the lower side of the paper surface in Fig. 1, respectively.
附图 1-4 示出了一种用于酶联免疫吸附测定的固相载体，这种固相载体是用于酶联免疫吸附测定的试剂盒的组成部分，其可应用于用于酶联免疫吸附测定的试剂盒中。 Figure 1-4 A solid phase carrier for enzyme-linked immunosorbent assay is shown, which is a component of a kit for enzyme-linked immunosorbent assays, which can be applied to reagents for enzyme-linked immunosorbent assays In the box.
参照附图 1-4 所示，该固相载体包括壳体以及自上至下依次设置在壳体内的纤维素膜 3 和衬垫 4 （一般由吸水材料构成），壳体的上部贯通开设有至少一个等压孔 11 ，且等压孔 11 不低于纤维素膜 3 ，优选为位于纤维素膜 3 的上方。具体到本实施例中，壳体由杯体 1 、杯盖 2 以及孵育杯 5 三部分构成，下面逐一对其进行详细描述。 Referring to Figures 1-4, the solid phase carrier includes a housing and a cellulose film 3 and a liner 4 disposed in the housing from top to bottom. (generally composed of a water absorbing material), at least one isopiped 11 is formed through the upper portion of the casing, and the isopipe 11 is not lower than the cellulose film 3, preferably located at the cellulose film 3 Above. Specifically, in the present embodiment, the casing is composed of a cup body 1, a cup cover 2, and an incubating cup 5, which will be described in detail below.
杯体 1 ，呈杯状。等压孔 11 设于杯体 1 上的靠近杯口的位置，除此之外，等压孔 11 还可设于杯盖 2 上。等压孔 11 的数量为至少一个，具体开设于杯体 1 的与孵育杯 5 相对的一侧壁上。在本实施例中，在杯盖 2 完全装配到杯体 1 上后，等压孔 11 位于纤维素膜 3 的上方，如附图 1 所示。等压孔 11 允许杯体 1 内的空气流出，消除了液体注入时产生的气弹簧效应，确保检测全程加液时的流入速度的一致性，使得检测结果较为准确，尤其是对于细长形的固相载体。 The cup 1 has a cup shape. The isopipe 11 is provided on the cup 1 near the mouth of the cup, and in addition, the isopipe 11 can be provided on the lid 2 on. The number of isopipes 11 is at least one, and is specifically formed on a side wall of the cup 1 opposite to the incubating cup 5. In the present embodiment, after the cup cover 2 is completely assembled to the cup 1, the isopipe 11 Located above the cellulose film 3, as shown in Figure 1. Isobaric hole 11 allows cup 1 The inside air flows out, eliminating the gas spring effect generated when the liquid is injected, ensuring the consistency of the inflow velocity when the whole process is added, so that the detection result is more accurate, especially for the elongated solid phase carrier.
杯体 1 内壁设有用于收缩衬垫 4 的凸棱 12 ，凸棱 12 优选为围绕杯体 1 纵向中心线一圈的封闭环形。凸棱 12 优选设置在杯体 1 的靠近杯口且处于等压孔 11 下方的位置。根据毛细张力作用和阀原理，在杯体 1 内壁设置凸棱 12 ，当嵌入吸水衬垫 4 时，形成的收口可抑制已经吸入杯体 1 内衬垫 4 的液体逆流向上，保证纤维素膜 3 的膜面过液体后处在一个正常的湿润状态，提高检测值的准确性。这可以避免：现有的固相载体吸液衬垫 4 接近饱和后逆行造成纤维素膜 3 的膜面出现液体残留，而导致的检测结果出现错误。 The inner wall of the cup 1 is provided with a rib 12 for shrinking the pad 4, and the rib 12 preferably surrounds the cup 1 A closed loop of the longitudinal centerline one turn. The rib 12 is preferably disposed at a position of the cup 1 near the cup opening and below the isopiped 11. According to the capillary tension action and the valve principle, a rib is provided on the inner wall of the cup body 12 When the water absorbing pad 4 is embedded, the formed cuff suppresses the liquid that has been sucked into the inner liner 4 of the cup body 1 to flow upward, and the cellulose film 3 is secured. The membrane surface is in a normal wet state after passing through the liquid, and the accuracy of the detection value is improved. This can be avoided: the existing solid phase carrier aspiration pad 4 is retrograde and then reversed to cause the cellulose film 3 There is liquid residue on the film surface, and the detection result is wrong.
为了使液体流动顺畅，杯体 1 内的空气上下流通，杯体 1 的底壁上贯通开设有小孔 13 。 In order to make the liquid flow smoothly, the air in the cup 1 flows up and down, and a small hole 13 is formed in the bottom wall of the cup body 1.
为了更好地固定衬垫 4 ，杯体 1 的底部设有朝向杯体 1 内部延伸的凸起 14 。凸起 14 优选设置在杯体 1 底部的中间位置。 In order to better secure the pad 4, the bottom of the cup 1 is provided with a projection 14 extending toward the inside of the cup 1. Raised 14 It is preferably disposed at an intermediate position at the bottom of the cup 1.
杯体 1 的垂直于其纵向中心线的截面呈矩形或圆角矩形，也就是说，杯体 1 大致呈长方体状或是倒圆角后的长方体状。这使得杯体 1 内具有允许衬垫 4 吸水后膨胀的空间，气弹簧效应相对较小。与之对应地，杯盖 2 的垂直于杯体 1 纵向中心线的截面呈矩形或圆角矩形。 The section of the cup 1 perpendicular to its longitudinal centerline has a rectangular or rounded rectangle, that is, the cup 1 It is roughly cuboid or rectangular in shape after rounding. This allows the cup 1 to have a space in which the pad 4 is allowed to swell and absorb, and the gas spring effect is relatively small. Correspondingly, the cup lid 2 is perpendicular to the cup 1 The longitudinal centerline has a rectangular or rounded rectangle.
杯盖 2 ，设于杯体 1 的杯口上。杯盖 2 呈漏斗形，杯盖 2 具有用于对液体进行引流的上表面，上表面为螺旋曲面 21 ，如附图 3 所示。相比现有技术中的斜面，螺旋曲面 21 可以对流向纤维素膜 3 表面的液体进行导向引流，使流向纤维素膜 3 的液体流速一定，液体和纤维素膜 3 表面具有较好的亲和性。 The lid 2 is provided on the cup mouth of the cup body 1. Cup lid 2 is funnel shaped, cup lid 2 There is an upper surface for draining the liquid, and the upper surface is a spiral curved surface 21 as shown in FIG. The spiral curved surface 21 can flow toward the cellulose film 3 compared to the bevel in the prior art. The liquid on the surface is guided to conduct a flow so that the flow rate of the liquid to the cellulose film 3 is constant, and the liquid and the surface of the cellulose film 3 have a good affinity.
杯盖 2 位于纤维素膜 3 和衬垫 4 上方，对纤维素膜 3 和衬垫 4 进行固定，保证两者紧密接触。杯盖 2 可容纳一定量的试剂投入，并保证所有试剂均通过中心区。 The lid 2 is located above the cellulose film 3 and the liner 4, against the cellulose film 3 and the liner 4 Fix it to ensure that the two are in close contact. The lid 2 accommodates a certain amount of reagent input and ensures that all reagents pass through the center.
杯盖 2 上开设有用于与杯体 1 的杯壁相配合卡接的一圈卡槽 22 ，卡槽 22 环绕为一圈，四面开槽，无方向性，方便杯盖 2 与杯体 1 的装配。 The cup cover 2 is provided with a ring slot 22 for engaging with the cup wall of the cup 1 , and the card slot 22 Surrounded by a circle, slotted on all sides, non-directional, convenient assembly of the lid 2 and the cup 1.
孵育杯 5 连接在杯体 1 的一侧，杯体 1 和孵育杯 5 一体成形。孵育杯 5 和等压孔 11 分别位于杯体 1 的相对两侧。将原来需要别加载的固相载体和孵育杯 5 两者一体化，简化了工序提高了自动化程度。而且，还避免了检测过程中杯体 1 内与孵育杯 5 内部的温度差给检测带来误差。 The incubating cup 5 is attached to one side of the cup body 1, and the cup body 1 and the incubating cup 5 are integrally formed. Incubation cup 5 and isobaric 11 Located on opposite sides of the cup 1 respectively. The integration of both the solid phase carrier and the incubation cup 5, which were originally required to be loaded, simplifies the process and increases the degree of automation. Moreover, the inside of the cup 1 and the incubating cup during the detection process are also avoided 5 The internal temperature difference introduces errors into the detection.
杯体 1 和 / 或孵育杯 5 的外壁上设有至少一个用于在加载时进行定位的凹槽 51 。凹槽 51 优选为成对设置，且分别位于杯体 1 和孵育杯 5 的连接处。杯体 1 和孵育杯 5 的外壁上开设有定位用的凹槽 51 ，方便加载。 The outer wall of the cup 1 and/or the incubating cup 5 is provided with at least one recess 51 for positioning during loading. Groove 51 Preferably, they are arranged in pairs and are respectively located at the junction of the cup 1 and the incubator cup 5. The outer wall of the cup body 1 and the incubating cup 5 is provided with a groove 51 for positioning for convenient loading.
上述实施例只为说明本发明的技术构思及特点，是一种优选的实施例，其目的在于熟悉此项技术的人士能够了解本发明的内容并据以实施，并不能以此限定本发明的保护范围。凡根据本发明的精神实质所作的等效变换或修饰，都应涵盖在本发明的保护范围之内。 The above embodiments are merely illustrative of the technical concept and features of the present invention, and are a preferred embodiment for those skilled in the art to understand the contents of the present invention and to implement the present invention. protected range. Equivalent transformations or modifications made in accordance with the spirit of the invention are intended to be included within the scope of the invention.

Claims (12)

  1. 一种用于酶联免疫吸附测定的固相载体，包括壳体以及自上至下依次设置在所述壳体内的纤维素膜和衬垫，其特征在于：所述壳体的上部贯通开设有至少一个等压孔，且所述等压孔不低于所述纤维素膜。A solid phase carrier for enzyme-linked immunosorbent assay, comprising a casing and a cellulose membrane and a liner disposed in the casing from top to bottom, wherein an upper portion of the casing is continuously opened At least one isobaric hole, and the isopiped is not lower than the cellulose film.
  2. 根据权利要求 1 所述的用于酶联免疫吸附测定的固相载体，其特征在于：所述壳体包括呈杯状的杯体，所述等压孔设于所述杯体上的靠近杯口的位置。According to claim 1 The solid phase carrier for enzyme-linked immunosorbent assay is characterized in that the housing comprises a cup-shaped cup body, and the isostatic hole is disposed on the cup body at a position close to the cup mouth.
  3. 根据权利要求 2 所述的用于酶联免疫吸附测定的固相载体，其特征在于：所述杯体内壁设有用于收缩所述衬垫的凸棱。According to claim 2 The solid phase carrier for enzyme-linked immunosorbent assay is characterized in that the inner wall of the cup is provided with a rib for shrinking the liner.
  4. 根据权利要求 3 所述的用于酶联免疫吸附测定的固相载体，其特征在于：所述凸棱为环形。The solid phase carrier for enzyme-linked immunosorbent assay according to claim 3, wherein the rib is annular.
  5. 根据权利要求 2 所述的用于酶联免疫吸附测定的固相载体，其特征在于：所述壳体还包括设于所述杯体的杯口上的杯盖，所述杯盖呈漏斗形，所述杯盖具有用于对液体进行引流的上表面，所述上表面为螺旋曲面。According to claim 2 The solid phase carrier for enzyme-linked immunosorbent assay, characterized in that the housing further comprises a lid provided on the cup mouth of the cup, the lid is funnel-shaped, the lid There is an upper surface for draining the liquid, the upper surface being a spiral curved surface.
  6. 根据权利要求 5 所述的用于酶联免疫吸附测定的固相载体，其特征在于：所述杯盖上开设有用于与所述杯体的杯壁相配合卡接的一圈卡槽。According to claim 5 The solid phase carrier for enzyme-linked immunosorbent assay is characterized in that: the cup lid is provided with a ring of card slots for engaging with the cup wall of the cup body.
  7. 根据权利要求 6 所述的用于酶联免疫吸附测定的固相载体，其特征在于：所述杯体和 / 或所述杯盖的垂直于杯体中心线的截面为矩形或圆角矩形。A solid phase carrier for enzyme-linked immunosorbent assay according to claim 6, wherein said cup and / Or the cross section of the lid perpendicular to the center line of the cup is a rectangle or a rounded rectangle.
  8. 根据权利要求 2 所述的用于酶联免疫吸附测定的固相载体，其特征在于：所述壳体还包括连接在所述杯体一侧的孵育杯，所述杯体和所述孵育杯一体成形。According to claim 2 The solid phase carrier for enzyme-linked immunosorbent assay, characterized in that the housing further comprises an incubating cup attached to one side of the cup, the cup and the incubating cup being integrally formed.
  9. 根据权利要求 8 所述的用于酶联免疫吸附测定的固相载体，其特征在于：所述杯体和 / 或所述孵育杯的外壁上设有至少一个用于在加载时进行定位的凹槽。A solid phase carrier for enzyme-linked immunosorbent assay according to claim 8, wherein said cup and / Or at least one groove for positioning during loading is provided on the outer wall of the incubator cup.
  10. 根据权利要求 2 所述的用于酶联免疫吸附测定的固相载体，其特征在于：所述杯体的底壁上贯通开设有小孔。According to claim 2 The solid phase carrier for enzyme-linked immunosorbent assay is characterized in that: a small hole is formed through the bottom wall of the cup body.
  11. 根据权利要求 2 所述的用于酶联免疫吸附测定的固相载体，其特征在于：所述杯体的底部设有朝向杯体内部延伸的凸起。According to claim 2 The solid phase carrier for enzyme-linked immunosorbent assay is characterized in that the bottom of the cup body is provided with a protrusion extending toward the inside of the cup body.
  12. 一种用于酶联免疫吸附测定的试剂盒，其特征在于：包括如权利要求 1-11 任一项所述的用于酶联免疫吸附测定的固相载体。A kit for enzyme-linked immunosorbent assay, comprising: according to claims 1-11 A solid phase carrier for enzyme-linked immunosorbent assay according to any one of the preceding claims.
PCT/CN2017/084311 2017-04-20 2017-05-15 Solid phase carrier and test kit used for enzyme-linked immunosorbent assay WO2018192036A1 (en)

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CN1547025A (en) * 2003-12-03 2004-11-17 刘春杰 Highly sensitive enzyme immunoassay detection kit
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CN102183653A (en) * 2011-03-21 2011-09-14 吉林大学 Cryptosporidium parvum immune colloidal gold detection test paper strip and production method thereof
CN102830233A (en) * 2011-06-13 2012-12-19 上海铭源数康生物芯片有限公司 ELISA reaction method based on cellulose nitrate membrane
CN103048457A (en) * 2012-11-27 2013-04-17 同昕生物技术（北京）有限公司 Myocardial infarction early-warning colloidal gold kit and preparation method thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1547025A (en) * 2003-12-03 2004-11-17 刘春杰 Highly sensitive enzyme immunoassay detection kit
CN101759800A (en) * 2009-12-22 2010-06-30 湖南大学 Preparation of dirhamnolipid artificial antigen and antibody, enzyme-linked immunity test paper, preparation and detection method thereof
CN101968489A (en) * 2010-08-25 2011-02-09 四川农业大学 Enzyme linked immunoassay kit for detecting sheep coenuruses
CN102183653A (en) * 2011-03-21 2011-09-14 吉林大学 Cryptosporidium parvum immune colloidal gold detection test paper strip and production method thereof
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