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WO2016005985A3 - Method for reprogramming cells - Google Patents

Method for reprogramming cells Download PDF

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Publication number
WO2016005985A3
WO2016005985A3 PCT/IL2015/050715 IL2015050715W WO2016005985A3 WO 2016005985 A3 WO2016005985 A3 WO 2016005985A3 IL 2015050715 W IL2015050715 W IL 2015050715W WO 2016005985 A3 WO2016005985 A3 WO 2016005985A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell
itsc
eomes
expressing
generating
Prior art date
Application number
PCT/IL2015/050715
Other languages
French (fr)
Other versions
WO2016005985A2 (en
Inventor
Yossi BUGANIM
Original Assignee
Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. filed Critical Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd.
Publication of WO2016005985A2 publication Critical patent/WO2016005985A2/en
Publication of WO2016005985A3 publication Critical patent/WO2016005985A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/606—Transcription factors c-Myc
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/91—Heparin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Gynecology & Obstetrics (AREA)
  • Biotechnology (AREA)
  • Reproductive Health (AREA)
  • Developmental Biology & Embryology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Pregnancy & Childbirth (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

A method of generating an induced trophoblast stem cell (iTSC) from a cell is provided. Accordingly there is provided a method comprising expressing within the cell at least one exogenous transcription factor selected from the group consisting of Gata3, Eomes and Tfap2c, under conditions which allow generation of an iTSC from the cell, thereby generating the iTSC from the cell, with the proviso that the method does not consist of expressing within the cell Eomes, Cdx2, Elf5, cMyc and Klf4. Also provided are nucleic acid constructs, isolated cells and iTSCs.
PCT/IL2015/050715 2014-07-09 2015-07-09 Method for reprogramming cells WO2016005985A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462022296P 2014-07-09 2014-07-09
US62/022,296 2014-07-09

Publications (2)

Publication Number Publication Date
WO2016005985A2 WO2016005985A2 (en) 2016-01-14
WO2016005985A3 true WO2016005985A3 (en) 2016-03-03

Family

ID=53872114

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2015/050715 WO2016005985A2 (en) 2014-07-09 2015-07-09 Method for reprogramming cells

Country Status (1)

Country Link
WO (1) WO2016005985A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105624194A (en) * 2016-02-16 2016-06-01 广东省农业科学院农业生物基因研究中心 Conditional Cas9 expression induced swine trophoblastic cell line and establishment method and application thereof
EP3768827A1 (en) * 2018-03-22 2021-01-27 INSERM (Institut National de la Santé et de la Recherche Médicale) Method for reprogramming somatic cells
WO2021092657A1 (en) * 2019-11-13 2021-05-20 Monash University Methods for reprogramming cells
EP4183873A4 (en) * 2020-07-17 2024-04-24 Tohoku University TROPHOBLAST STEM CELL-LIKE CELLS FOR DIFFERENTIATION INTO PLACENTA-CONSTITUTING CELLS AND METHODS FOR THE PRODUCTION THEREOF
US20240301019A1 (en) * 2021-02-15 2024-09-12 Arizona Board Of Regents On Behalf Of The University Of Arizona Recombinant eomes restores anti-cancer activity of immune cells
IL309333A (en) 2021-06-13 2024-02-01 Yissum Res Dev Co Of Hebrew Univ Jerusalem Ltd Method for reprogramming human cells

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