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WO2015173774A3 - A microscopy system and a method for analyzing fluids - Google Patents

A microscopy system and a method for analyzing fluids Download PDF

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Publication number
WO2015173774A3
WO2015173774A3 PCT/IB2015/053581 IB2015053581W WO2015173774A3 WO 2015173774 A3 WO2015173774 A3 WO 2015173774A3 IB 2015053581 W IB2015053581 W IB 2015053581W WO 2015173774 A3 WO2015173774 A3 WO 2015173774A3
Authority
WO
WIPO (PCT)
Prior art keywords
microscopy system
fluids
zone
mixture
imaging
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2015/053581
Other languages
French (fr)
Other versions
WO2015173774A2 (en
Inventor
Siva Gorthi SAI
Kalyan Jagannadh VEERENDRA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Indian Institute of Science IISC
Original Assignee
Indian Institute of Science IISC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Indian Institute of Science IISC filed Critical Indian Institute of Science IISC
Publication of WO2015173774A2 publication Critical patent/WO2015173774A2/en
Publication of WO2015173774A3 publication Critical patent/WO2015173774A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/05—Flow-through cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/47—Scattering, i.e. diffuse reflection
    • G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
    • G01N21/53—Scattering, i.e. diffuse reflection within a body or fluid within a flowing fluid, e.g. smoke
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0325—Cells for testing reactions, e.g. containing reagents
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0346—Capillary cells; Microcells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00099—Characterised by type of test elements
    • G01N2035/00158—Elements containing microarrays, i.e. "biochip"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/02—Mechanical
    • G01N2201/022—Casings
    • G01N2201/0221—Portable; cableless; compact; hand-held

Landscapes

  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plasma & Fusion (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

Embodiments of the present disclosure relate to a microscopy system and a method to analyze fluids in flow. The microscopy system comprises a microfluidic cartridge to receive input comprising at least one of fluids and reagents. The microfluidic cartridge further comprises a mixing zone to receive the at least one of fluids and reagents to form a mixture and an imaging zone to receive the mixture from the mixing zone. An illumination unit is configured in the microscopy system to illuminate the imaging zone and at least one imaging unit to capture plurality of images of one or more microscopic elements of the mixture flowing through the imaging zone. The plurality of images is processed by a computing unit associated with the microscopy system for analyzing fluids.
PCT/IB2015/053581 2014-05-15 2015-05-15 A microscopy system and a method for analyzing fluids Ceased WO2015173774A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN2432/CHE/2014 2014-05-15
IN2432CH2014 IN2014CH02432A (en) 2014-05-15 2015-05-15

Publications (2)

Publication Number Publication Date
WO2015173774A2 WO2015173774A2 (en) 2015-11-19
WO2015173774A3 true WO2015173774A3 (en) 2016-01-21

Family

ID=53835484

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2015/053581 Ceased WO2015173774A2 (en) 2014-05-15 2015-05-15 A microscopy system and a method for analyzing fluids

Country Status (2)

Country Link
IN (1) IN2014CH02432A (en)
WO (1) WO2015173774A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11478789B2 (en) 2014-11-26 2022-10-25 Medica Corporation Automated microscopic cell analysis
US20170328924A1 (en) 2014-11-26 2017-11-16 Ronald Jones Automated microscopic cell analysis
US10625259B1 (en) 2014-11-26 2020-04-21 Medica Corporation Automated microscopic cell analysis
US12005441B1 (en) 2014-11-26 2024-06-11 Medica Corporation Automated microscopic cell analysis
WO2018009920A1 (en) 2016-07-08 2018-01-11 Medica Corporation Automated microscopic cell analysis
CN107714096A (en) * 2016-08-10 2018-02-23 量准（上海）医疗科技有限公司 A kind of quantitative detection ovulation device and detection method
WO2018142415A1 (en) * 2017-02-06 2018-08-09 Efa - Engineering For All Ltd. Portable digital diagnostic device
US20240001359A1 (en) * 2020-11-27 2024-01-04 Indian Institute Of Technology Delhi Microfluidic analyser for in-vitro biosensing and diagnostics

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070190525A1 (en) * 2000-06-02 2007-08-16 Honeywell International Inc. Assay implementation in a microfluidic format
WO2012032294A1 (en) * 2010-09-07 2012-03-15 Multi-Sense Technologies Limited Assay device and reader
EP2444157A1 (en) * 2010-10-25 2012-04-25 Danmarks Tekniske Universitet - DTU Microfluidic device and method for enzymatic processing of macromolecules
US20130273524A1 (en) * 2012-04-17 2013-10-17 Joel R. L. Ehrenkranz Device for performing a blood, cell, and/or pathogen count and methods for use thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070190525A1 (en) * 2000-06-02 2007-08-16 Honeywell International Inc. Assay implementation in a microfluidic format
WO2012032294A1 (en) * 2010-09-07 2012-03-15 Multi-Sense Technologies Limited Assay device and reader
EP2444157A1 (en) * 2010-10-25 2012-04-25 Danmarks Tekniske Universitet - DTU Microfluidic device and method for enzymatic processing of macromolecules
US20130273524A1 (en) * 2012-04-17 2013-10-17 Joel R. L. Ehrenkranz Device for performing a blood, cell, and/or pathogen count and methods for use thereof

Also Published As

Publication number Publication date
IN2014CH02432A (en) 2016-01-15
WO2015173774A2 (en) 2015-11-19

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