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WO2015074499A1 - Method for producing fermented honey beverage and products thereof - Google Patents

Method for producing fermented honey beverage and products thereof Download PDF

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Publication number
WO2015074499A1
WO2015074499A1 PCT/CN2014/090684 CN2014090684W WO2015074499A1 WO 2015074499 A1 WO2015074499 A1 WO 2015074499A1 CN 2014090684 W CN2014090684 W CN 2014090684W WO 2015074499 A1 WO2015074499 A1 WO 2015074499A1
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Prior art keywords
honey
liquid
fermented
acetic acid
acid bacteria
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PCT/CN2014/090684
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French (fr)
Chinese (zh)
Inventor
曲都
冯彬
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成都冯氏蜂业有限公司
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Publication of WO2015074499A1 publication Critical patent/WO2015074499A1/en

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
    • A23L2/38Other non-alcoholic beverages
    • A23L2/382Other non-alcoholic beverages fermented
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
    • A23L2/70Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/84Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L21/00Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
    • A23L21/20Products from apiculture, e.g. royal jelly or pollen; Substitutes therefor
    • A23L21/25Honey; Honey substitutes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

Definitions

  • the present invention relates to a method for producing a beverage, and more particularly to a method for producing a honey fermented beverage and a product prepared by the method.
  • Honey is a pure natural, health-care sugar complex with rich nutrients, but most of the dry matter in pure honey is sugar, accounting for more than 90% of dry matter. Direct drinking of honey is unacceptable for most people, and it is difficult to swallow, and excessive intake of carbohydrates is not beneficial to health. All kinds of beverage products on the market are currently prepared with various food additives. Pure honey and pollen are used to ferment the three probiotics to convert the sugar substances into acetic acid, lactic acid and amino acids, so as to form a healthy drink with no added additives, pure natural taste and sweet and sour taste. To fill the gap in China's pure natural honey fermented beverages.
  • the technical problem to be solved by the present invention is to provide a method for producing a honey fermented beverage in view of the problems existing in the prior art in preparing a beverage.
  • the invention discloses a method for producing a honey fermented beverage, wherein the raw material is filtered to remove impurities, and the total sugar content is measured, and then diluted with pure water to obtain a honey dilution solution, then added with pollen, stirred uniformly, and then heated to 75 ° C.
  • the mass concentration of honey in the honey dilution is 20%; the mass ratio of the added pollen to honey dilution is 1 ⁇ .
  • the low-temperature yeast domestication liquid is prepared by acclimating EC-1118 Saccharomyces cerevisiae by the invention patent 201310039186.7, and the volume concentration of the low-temperature yeast domestication liquid in the honey dilution liquid is 4%; the acetic acid domestication liquid It was prepared by domesticating the 1.07 acetic acid bacteria with the invention patent 201310039186.7, and the volume concentration of the acetic acid acclimation liquid in the honey dilution was 11%.
  • the lactic acid bacteria group is a Lactobacillus acidophilus mixed with a mass ratio of 1:1:1, which is also called a biguanide factor and a Lactobacillus plantarum; the volume concentration of the lactic acid bacteria group in the honey diluent is 1 ⁇ . .
  • the honey fermented beverage product has a total sugar value of 4% to 8% and a total acid value of 0.5 g/100 ml to 1.0 g/100 ml.
  • the technology of the invention has its distinct features: First, the use of pure honey, pollen, without adding any other food additives to ensure the naturalness of the product. Second, the use of three probiotic fermentation, in the fermentation process will produce a lot of beneficial substances, rather than artificially added. Third, the precise control of fermentation, reasonable maintenance of the proportion of the various products of the product, do a good taste, beautiful color. Fourth, the nutritional value of the beverage is high.
  • the temperature was raised to 75 ° C for 30 minutes and then cooled to 25 ° C.
  • a low temperature yeast acclimation solution was added, which had a volume concentration of 4% in the honey dilution; on the first day, the fermentor was opened and the temperature was maintained at 25 °C. The next day, sealed. Keep at 25 °C.
  • the alcohol content was sampled on the fourth day and measured once a day. When the alcohol content reached a volume concentration of 4%, the fermentation was immediately terminated, and the temperature was raised to 75 ° C for 30 minutes to inactivate the yeast.
  • the acclimation liquid which has a volume concentration of 11% in the honey dilution, maintains the oxygen supply state, maintains the temperature at 37 ° C, and ferments. From the seventh day, the fermentation broth was observed, and when it turned reddish brown, the total acidity (acetic acid) was measured daily. When the total acidity reached 2.0 g/100 ml, the lactic acid bacteria group was added in a volume concentration of 1 Torr in the honey diluent. When the alcohol is burned to the natural atmosphere, the fermenter is immediately sealed, the temperature is maintained at 37 ° C, and the total acid value is measured the next day.
  • the total acidity exceeds 2.5 g / 100 ml, it is immediately filtered. The filtrate was boiled for 30 minutes. Then determine the total sugar, total acid, alcohol. The total sugar value of the fermentation broth was maintained at 15% by the dilution and addition of pure honey, and the total acidity was 2.5 g/100 ml. After diluting 4 times with purified water, the total sugar value was determined to be 6%, and the total acid value was 0.6 g/100 ml.
  • Homogenization is carried out by a high pressure homogenizer. Then canned and enter the sterilization box at 100 ° C for 15 minutes. The air is cooled to below 45 ° C to obtain the finished product.
  • the low-temperature yeast domestication liquid was prepared by acclimating EC-1118 Saccharomyces cerevisiae with the invention patent 201310039186.7; the acetic acid domestication liquid was prepared by domesticating the 1.01 acetic acid bacteria by the invention patent 201310039186.7.
  • the lactic acid bacteria group is Lactobacillus acidophilus, Bifidobacterium and Lactobacillus plantarum mixed at a mass ratio of 1:1:1.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Mycology (AREA)
  • Non-Alcoholic Beverages (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Disclosed is a method for producing a fermented honey beverage, and the method comprises the steps of: filtering the raw honey to removing impurities, then diluting with pure water, adding pollen again, followed by stepwise adding a low temperature yeast domestication liquid, acetic acid domestication liquid and Lactobacillus flora, and fermentation.

Description

一种蜂蜜发酵饮料的生产方法及其产品Method for producing honey fermented beverage and product thereof 技术领域Technical field
本发明涉及饮料的生产方法,尤其涉及的是一种蜂蜜发酵饮料的生产方法和由此方法制备出的产品。The present invention relates to a method for producing a beverage, and more particularly to a method for producing a honey fermented beverage and a product prepared by the method.
背景技术Background technique
蜂蜜是一种纯天然,极具保健功能的糖类复合体,营养成份丰富,但纯蜂蜜中绝大部份干物质为糖类物质,占干物质的90%以上。直接饮用蜂蜜绝大部份人群无法接受,而且难以下咽,过多摄入糖类物质无利健康。目前市场上各种饮料产品全部都用各种食品添加剂调制而成。用纯蜂蜜、花粉通过三种益生菌的发酵过程,将其中的糖类物质,转换成乙酸、乳酸及产生氨基酸,从而形成不添加任何添加物,纯天然的、口感酸酸甜甜的养生饮品,填补了我国纯天然蜂蜜发酵饮料的空白。Honey is a pure natural, health-care sugar complex with rich nutrients, but most of the dry matter in pure honey is sugar, accounting for more than 90% of dry matter. Direct drinking of honey is unacceptable for most people, and it is difficult to swallow, and excessive intake of carbohydrates is not beneficial to health. All kinds of beverage products on the market are currently prepared with various food additives. Pure honey and pollen are used to ferment the three probiotics to convert the sugar substances into acetic acid, lactic acid and amino acids, so as to form a healthy drink with no added additives, pure natural taste and sweet and sour taste. To fill the gap in China's pure natural honey fermented beverages.
发明内容Summary of the invention
本发明所要解决的技术问题是针对现有技术在制备饮料中存在的问题,提供了一种蜂蜜发酵饮料的生产方法。The technical problem to be solved by the present invention is to provide a method for producing a honey fermented beverage in view of the problems existing in the prior art in preparing a beverage.
本发明的技术方案如下:The technical solution of the present invention is as follows:
一种蜂蜜发酵饮料的生产方法,其技术方案为,采原蜜过滤去杂质,测定其总糖含量值,然后用纯净水稀释得到蜂蜜稀释液,再加入花粉,搅拌均匀,然后升温至75℃,保持30分钟后冷却至25℃;加入低温酵母菌驯化液,25℃密闭发酵,精确发酵至酒精度为4,立即升温至75℃,30分钟灭活酵母菌;降温至40℃后加入醋酸菌驯化液,37℃供氧状态下,精确发酵至总酸值为2克/100毫升,冲入CO2,排去氧气;之后加入乳酸菌群,密封、37℃保持24小时,总酸值上升0.5克/100毫升终止,立即过滤后,将滤 液煮沸30分钟;用纯净水稀释4倍,得到蜂蜜发酵饮料产品。The invention discloses a method for producing a honey fermented beverage, wherein the raw material is filtered to remove impurities, and the total sugar content is measured, and then diluted with pure water to obtain a honey dilution solution, then added with pollen, stirred uniformly, and then heated to 75 ° C. After 30 minutes, cool to 25 ° C; add low-temperature yeast acclimation liquid, closed fermentation at 25 ° C, accurately ferment until the alcohol level is 4, immediately heat up to 75 ° C, inactivate the yeast for 30 minutes; after cooling to 40 ° C, add acetic acid The acclimation solution of bacteria is accurately fermented to a total acid value of 2 g / 100 ml at 37 ° C, flushed into CO 2 and discharged with oxygen; then added to the lactic acid bacteria group, sealed, kept at 37 ° C for 24 hours, the total acid value rises After termination of 0.5 g/100 ml, immediately after filtration, the filtrate was boiled for 30 minutes; diluted 4 times with purified water to obtain a honey fermented beverage product.
所述的生产方法,蜂蜜稀释液中蜂蜜的质量浓度为20%;加入的花粉和蜂蜜稀释液的质量比是1‰。In the production method, the mass concentration of honey in the honey dilution is 20%; the mass ratio of the added pollen to honey dilution is 1‰.
所述的生产方法,低温酵母菌驯化液是采用发明专利201310039186.7对EC-1118酿酒酵母进行驯化而制得的,低温酵母菌驯化液在蜂蜜稀释液中的体积浓度为4%;醋酸菌驯化液是采用发明专利201310039186.7对沪酿1.01醋酸菌进行驯化而制得的,醋酸菌驯化液在蜂蜜稀释液中的体积浓度为11%。The production method, the low-temperature yeast domestication liquid is prepared by acclimating EC-1118 Saccharomyces cerevisiae by the invention patent 201310039186.7, and the volume concentration of the low-temperature yeast domestication liquid in the honey dilution liquid is 4%; the acetic acid domestication liquid It was prepared by domesticating the 1.07 acetic acid bacteria with the invention patent 201310039186.7, and the volume concentration of the acetic acid acclimation liquid in the honey dilution was 11%.
所述的生产方法,乳酸菌群是按质量比1:1:1混合的嗜酸乳杆菌、双岐杆菌又称双岐因子和植物乳杆菌;乳酸菌群在蜂蜜稀释液中的体积浓度为1‰。In the production method, the lactic acid bacteria group is a Lactobacillus acidophilus mixed with a mass ratio of 1:1:1, which is also called a biguanide factor and a Lactobacillus plantarum; the volume concentration of the lactic acid bacteria group in the honey diluent is 1‰. .
所述的生产方法,蜂蜜发酵饮料产品的总糖值为4%-8%,总酸值为0.5克/100毫升-1.0克/100毫升。In the production method, the honey fermented beverage product has a total sugar value of 4% to 8% and a total acid value of 0.5 g/100 ml to 1.0 g/100 ml.
本发明技术具有其鲜明特点:一、采用纯蜂蜜、花粉,不添加任何其它食品添加剂,确保产品的纯天然性。二、采用三种益生菌发酵,在发酵过程中会产生许多有益物质,而不是人为添加。三、发酵精确控制,合理保持产品的各物比例协调,做倒口感好、色泽美观。四、饮料营养价值高。The technology of the invention has its distinct features: First, the use of pure honey, pollen, without adding any other food additives to ensure the naturalness of the product. Second, the use of three probiotic fermentation, in the fermentation process will produce a lot of beneficial substances, rather than artificially added. Third, the precise control of fermentation, reasonable maintenance of the proportion of the various products of the product, do a good taste, beautiful color. Fourth, the nutritional value of the beverage is high.
具体实施方式detailed description
以下结合具体实施例,对本发明进行详细说明。The present invention will be described in detail below in conjunction with specific embodiments.
实施例Example
选择色泽金黄、有自然花香的山花蜂蜜,过滤去掉混于其中的杂质。通过除蜡工艺,去掉其中蜂蜡成份。测定其总糖含量为70%,根据其总糖值计算用纯水稀至总糖含量为20%。加入纯花粉,其质量为蜂蜜稀释液总质量的1‰,搅拌均匀。Choose a honey flower with golden color and natural floral fragrance, and filter to remove impurities mixed in it. The beeswax component is removed by a wax removal process. The total sugar content was determined to be 70%, and it was diluted with pure water to a total sugar content of 20% based on the total sugar value. Add pure pollen, the quality of which is 1‰ of the total mass of the honey dilution, and stir evenly.
升温至75℃,保持30分钟,然后冷却至25℃。加入低温酵母菌驯化液,其在蜂蜜稀释液中的体积浓度为4%;第一天,打开发酵罐,保持温度25℃。第二天,密闭。保持25℃。第四天起抽样测定其酒精含量,每天测定一次。酒精含量达到体积浓度为4%时,立即终止发酵,将温度升至75℃、30分钟灭活酵母菌。降温至40℃,加入醋酸菌 驯化液,其在蜂蜜稀释液中的体积浓度为11%,保持供氧状态,温度保持37℃,发酵。第七天起,观察其发酵液,转为红棕色时,每日测定其总酸度(乙酸计)。总酸度达到2.0克/100毫升时,加入乳酸菌群,其在蜂蜜稀释液中的体积浓度为1‰。采用酒精燃烧至自然息灭时,立即密闭发酵罐,保持温度37℃,第二天测定总酸值,当总酸度超过2.5克/100毫升时,立即过滤。将滤液煮沸30分钟。然后测定其总糖、总酸、酒精度。通过稀释、加入纯蜂蜜方式保持其发酵液总糖值15%、总酸度2.5克/100毫升。用纯净水稀释4倍后,测定总糖值为6%、总酸值0.6克/100毫升。The temperature was raised to 75 ° C for 30 minutes and then cooled to 25 ° C. A low temperature yeast acclimation solution was added, which had a volume concentration of 4% in the honey dilution; on the first day, the fermentor was opened and the temperature was maintained at 25 °C. The next day, sealed. Keep at 25 °C. The alcohol content was sampled on the fourth day and measured once a day. When the alcohol content reached a volume concentration of 4%, the fermentation was immediately terminated, and the temperature was raised to 75 ° C for 30 minutes to inactivate the yeast. Cool down to 40 ° C, add acetic acid bacteria The acclimation liquid, which has a volume concentration of 11% in the honey dilution, maintains the oxygen supply state, maintains the temperature at 37 ° C, and ferments. From the seventh day, the fermentation broth was observed, and when it turned reddish brown, the total acidity (acetic acid) was measured daily. When the total acidity reached 2.0 g/100 ml, the lactic acid bacteria group was added in a volume concentration of 1 Torr in the honey diluent. When the alcohol is burned to the natural atmosphere, the fermenter is immediately sealed, the temperature is maintained at 37 ° C, and the total acid value is measured the next day. When the total acidity exceeds 2.5 g / 100 ml, it is immediately filtered. The filtrate was boiled for 30 minutes. Then determine the total sugar, total acid, alcohol. The total sugar value of the fermentation broth was maintained at 15% by the dilution and addition of pure honey, and the total acidity was 2.5 g/100 ml. After diluting 4 times with purified water, the total sugar value was determined to be 6%, and the total acid value was 0.6 g/100 ml.
通过高压匀质机,进行匀质。然后罐装,进入灭菌箱,100℃、15分钟。风冷到45℃以下,得到成品。Homogenization is carried out by a high pressure homogenizer. Then canned and enter the sterilization box at 100 ° C for 15 minutes. The air is cooled to below 45 ° C to obtain the finished product.
其中,低温酵母菌驯化液是采用发明专利201310039186.7对EC-1118酿酒酵母进行驯化而制得的;醋酸菌驯化液是采用发明专利201310039186.7对沪酿1.01醋酸菌进行驯化而制得的。Among them, the low-temperature yeast domestication liquid was prepared by acclimating EC-1118 Saccharomyces cerevisiae with the invention patent 201310039186.7; the acetic acid domestication liquid was prepared by domesticating the 1.01 acetic acid bacteria by the invention patent 201310039186.7.
乳酸菌群是按质量比1:1:1混合的嗜酸乳杆菌、双岐杆菌和植物乳杆菌。The lactic acid bacteria group is Lactobacillus acidophilus, Bifidobacterium and Lactobacillus plantarum mixed at a mass ratio of 1:1:1.
应当理解的是,对本领域普通技术人员来说,可以根据上述说明加以改进或变换,而所有这些改进和变换都应属于本发明所附权利要求的保护范围。 It is to be understood that those skilled in the art will be able to make modifications and changes in accordance with the above description, and all such modifications and variations are intended to be included within the scope of the appended claims.

Claims (6)

  1. 一种蜂蜜发酵饮料的生产方法,其特征是,采原蜜过滤去杂质,测定其总糖含量值,然后用纯净水稀释得到蜂蜜稀释液,再加入花粉,搅拌均匀,然后升温至75℃,保持30分钟后冷却至25℃;加入低温酵母菌驯化液,25℃密闭发酵,精确发酵至酒精度为4,立即升温至75℃,30分钟灭活酵母菌;降温至40℃后加入醋酸菌驯化液,37℃供氧状态下,精确发酵至总酸值为2克/100毫升,冲入CO2,排去氧气;之后加入乳酸菌群,密封、37℃保持24小时,总酸值上升0.5克/100毫升终止,立即过滤后,将滤液煮沸30分钟;用纯净水稀释4倍,得到蜂蜜发酵饮料产品。The invention relates to a method for producing a honey fermented beverage, which is characterized in that the original honey is filtered to remove impurities, and the total sugar content value is measured, and then diluted with pure water to obtain a honey dilution liquid, and then the pollen is added, stirred uniformly, and then heated to 75 ° C, After 30 minutes, cool to 25 ° C; add low-temperature yeast domestication solution, closed fermentation at 25 ° C, accurately fermented to 4 degrees of alcohol, immediately warmed to 75 ° C, 30 minutes to inactivate yeast; after cooling to 40 ° C, add acetic acid bacteria Acclimation liquid, fermented at 37 ° C, accurately fermented to a total acid value of 2 g / 100 ml, flushed into CO 2 , exhausted oxygen; then added lactic acid bacteria, sealed, maintained at 37 ° C for 24 hours, total acid value increased by 0.5 The gram/100 ml was terminated, and immediately filtered, the filtrate was boiled for 30 minutes; diluted 4 times with purified water to obtain a honey fermented beverage product.
  2. 根据权利要求1所述的生产方法,其特征是,蜂蜜稀释液中蜂蜜的质量浓度为20%;加入的花粉和蜂蜜稀释液的质量比是1‰。The production method according to Claim 1, wherein the mass concentration of honey in the honey diluent is 20%; and the mass ratio of the added pollen to honey diluent is 1 Torr.
  3. 根据权利要求1所述的生产方法,其特征是,低温酵母菌驯化液是采用发明专利201310039186.7对EC-1118酿酒酵母进行驯化而制得的,低温酵母菌驯化液在蜂蜜稀释液中的体积浓度为4%;醋酸菌驯化液是采用发明专利201310039186.7对沪酿1.01醋酸菌进行驯化而制得的,醋酸菌驯化液在蜂蜜稀释液中的体积浓度为11%。The production method according to claim 1, wherein the low-temperature yeast domestication liquid is prepared by acclimating EC-1118 Saccharomyces cerevisiae using the invention patent 201310039186.7, and the volume concentration of the low-temperature yeast domestication liquid in the honey dilution liquid It is 4%; the acetic acid acclimation liquid is prepared by domesticating the 1.07 acetic acid bacteria by the invention patent 201310039186.7, and the volume concentration of the acetic acid acclimation liquid in the honey dilution is 11%.
  4. 根据权利要求1所述的生产方法,其特征是,乳酸菌群是按质量比1:1:1混合的嗜酸乳杆菌、双岐杆菌又称双岐因子和植物乳杆菌;乳酸菌群在蜂蜜稀释液中的体积浓度为1‰。The production method according to claim 1, wherein the lactic acid bacteria group is a Lactobacillus acidophilus mixed with a mass ratio of 1:1:1, and the Bifidobacterium bacillus is also called a biguanide factor and a Lactobacillus plantarum; the lactic acid bacteria group is diluted in honey. The volume concentration in the liquid is 1 Torr.
  5. 根据权利要求1所述的生产方法,其特征是,蜂蜜发酵饮料产品的总糖值为4%-8%,总酸值为0.5克/100毫升-1.0克/100毫升。The production method according to claim 1, wherein the honey fermented beverage product has a total sugar value of 4% to 8% and a total acid value of 0.5 g/100 ml to 1.0 g/100 ml.
  6. 根据权利要求1至5至一所述的方法制备的蜂蜜发酵饮料。 A honey fermented beverage prepared according to the method of claims 1 to 5 to 1.
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