WO2015037720A1 - Muscle repair promoter - Google Patents

Abstract

　Provided is a muscle repair promoter intended for use in a target of which rapid absorption of protein is required, and containing as an effective ingredient a lactoprotein mixture that includes casein protein and whey protein, wherein the muscle repair promoter has a protein ratio of casein protein to whey protein (casein protein/whey protein) of 2-20. The muscle repair promoter exhibits rapid muscle repair promoting action equivalent to that of whey protein.

Description

Muscle synthesis promoter Background of the Invention

This patent application is accompanied by a claim of priority based on Japanese Patent Application No. 2013-189703 (application date: September 12, 2013), which was a previously filed Japanese patent application. The entire disclosure of this earlier patent application is incorporated herein by reference.

TECHNICAL FIELD The present invention relates to muscle synthesis promoters for subjects in need of immediate protein absorption.

Background Art In recent years, for the purpose of promoting health and improving exercise capacity, orally ingesting dietary supplements (protein supplements) based on protein to promote muscle synthesis, prevent muscle loss, and promote muscle mass recovery. Many people (subjects) exercise. As such a nutritional supplement, there is protein powder, and as its main component, whey protein (whey protein) and casein protein made from milk are known.

Whey protein has a high rate (absorption time) from ingestion to digestion and absorption (short time) and contains a lot of BCAA (branched chain amino acids: valine, leucine, isoleucine), thus preventing muscle fatigue, It is said that muscles can be strengthened efficiently. On the other hand, casein protein is slowly and continuously metabolized compared to whey protein, so the rate from oral ingestion to digestion absorption (absorption rate) is slow (longer time) and needs immediate absorption. Is said to be unsuitable (see Patent Document 1). Therefore, whey protein is mainly used as protein powder.

Utilizing such a difference in absorption rate, a mixed protein in which whey protein having high absorption and casein protein to be continuously absorbed are mixed at 1: 1 has been developed (for example, DNS protein slow loading). ).

However, since whey protein has low heat resistance (thermal stability), it undergoes thermal denaturation and tends to aggregate due to heating. In fact, protein powders are not recommended for consumption other than being dissolved in water. Therefore, protein powder remains powdery and cannot be said to have high palatability. Furthermore, when heated excessively, the whey protein is hardened by heat denaturation and there is a problem that the absorption efficiency is lowered.

Special table 2009-507044 gazette

Thus, materials and compositions having high heat resistance and good processing suitability while exhibiting an immediate muscle synthesis promoting effect equivalent to whey protein have not been known so far.

In the prior art, the present inventors have now proposed a milk protein mixture in which the majority of whey protein is replaced by casein protein, which has been said to have a slow protein absorption rate and no immediate effect like whey protein. Unexpectedly, it was found that there is an immediate effect of promoting muscle synthesis equivalent to whey protein. Milk protein consisting of about 80% casein protein and about 20% whey protein was generally known to have the same absorbency as casein protein alone (Judy A. Driskell, "Sports Nutrition Fats and proteins" Therefore, the above phenomenon was surprising. It was also found that this milk protein mixture has higher heat resistance and better processability than using whey protein alone. The present invention is based on these findings.

That is, an object of the present invention is to provide a muscle synthesis (enhancement) promoter having an immediate effect of promoting muscle synthesis equivalent to that of whey protein.

According to the present invention, the following inventions are provided.
(1) A muscle synthesis promoter for a subject in need of immediate protein absorption, comprising a milk protein mixture containing casein protein and whey protein as an active ingredient, and the amount of casein protein relative to whey protein A muscle synthesis promoter having a ratio (casein protein / whey protein) of 2 to 20.
(2) The muscle synthesis promoter according to (1) above, wherein the milk protein mixture is taken so that the total protein amount is 0.13 to 0.7 g / kg body weight per serving.
(3) The above-mentioned (1) or (2), comprising a unit packaging form per serving, wherein the milk protein mixture is adjusted to 8 to 40 g in terms of the total protein amount as a meal intake. The muscle synthesis promoter described in 1.
(4) The muscle synthesis promoter according to any one of (1) to (3) above, wherein the subject in need of immediate protein absorption is a subject to exercise.
(5) The muscle synthesis promoter according to (4) above, wherein the exercise is muscle training.
(6) The muscle synthesis promoter according to any one of (1) to (5), which is in a liquid form.
(7) A food / beverage product obtained by blending and heat-sterilizing the muscle synthesis promoter according to any one of (1) to (6) above, comprising a unit packaging form per serving, and in the unit packaging form A food and drink packaged in a packaging container, provided with an effect of promoting muscle synthesis by immediate absorption of protein, wherein the milk protein mixture is adjusted to 8 to 40 g in terms of the total amount of protein as a single meal intake.
(8) A milk protein mixture containing casein protein and whey protein having a protein amount ratio of casein protein to whey protein (casein protein / whey protein) of 2 to 20, raw milk, pasteurized milk, concentrated milk, milk powder and reduced milk A milk beverage mixed and heat sterilized with at least one selected from the group consisting of milk, wherein the milk protein mixture is adjusted to a total protein content of 8 g / 200 ml and a milk fat content of 8 g / 200 ml A packaged container-packed milk drink that is provided with an action for promoting muscle synthesis by immediate protein absorption.
(9) A milk protein mixture containing casein protein and whey protein having a protein amount ratio of casein protein to whey protein (casein protein / whey protein) of 2 to 20, raw milk, pasteurized milk, concentrated milk, milk powder and reduced milk A method for producing a packaged-packed milk beverage comprising a step of adding to one or more selected from the group consisting of milk and a step of heat sterilization, wherein the milk protein mixture is 8 g / A method for producing a packaged-packed milk drink, which is provided with an effect of promoting muscle synthesis by immediate absorption of protein, wherein 200 ml and the amount of milk fat are adjusted to 8 g / 200 ml or less.
(10) A milk protein mixture containing casein protein and whey protein having a protein amount ratio of casein protein to whey protein (casein protein / whey protein) of 2 to 20 is taken by a subject who needs immediate absorption of the protein A method of promoting muscle synthesis, comprising:
(11) A milk protein mixture containing casein protein and whey protein having a protein amount ratio of casein protein to whey protein (casein protein / whey protein) of 2 to 20 is taken by a subject who needs immediate absorption of the protein. A method for promoting muscle synthesis, comprising:
(12) The method for promoting muscle synthesis according to (10) or (11) above, wherein the milk protein mixture is ingested so that the total protein amount is 0.13 to 0.7 g / kg body weight per serving.
(13) The method for promoting muscle synthesis according to (12), wherein the milk protein mixture is ingested so that the total protein amount of the milk protein mixture is 8 to 40 g per serving.
(14) The method for promoting muscle synthesis according to any one of the above (10) to (13), comprising ingesting the milk protein mixture in a heated liquid form to a subject in need of immediate absorption of the protein.
(15) The method for promoting muscle synthesis according to any one of (10) to (14) above, wherein the subject in need of immediate protein absorption is a subject to exercise.
(16) The method for promoting muscle synthesis according to (15), wherein the object to be exercised is an object immediately after exercise.
(17) The method for promoting muscle synthesis according to (15) or (16) above, wherein the exercise is muscle training.

The muscle synthesis promoter of the present invention has an immediate effect of promoting muscle synthesis equivalent to that of whey protein, and has higher heat resistance and better processability than using whey protein alone. Therefore, the muscle synthesis promoter of the present invention is advantageous in that it can be expected to have an immediate effect of promoting muscle synthesis and can be provided as an inexpensive product form that can be mass-produced commercially.

FIG. 1 shows the skeletal muscle synthesis rate (FSR) in the triceps surae muscle and the hyfuku muscle when orally ingesting (administering) milk protein solutions of various concentrations. FIG. 2 shows changes in FSR with swimming exercise. FIG. 3 shows the blood 3MeHis concentration when each concentration of milk protein solution was orally ingested (administered).

Detailed description of the invention

The muscle synthesis promoter of the present invention is a muscle synthesis promoter for a subject in need of immediate protein absorption, comprising a milk protein mixture containing casein protein and whey protein as an active ingredient. It is a muscle synthesis promoter having a protein amount ratio (casein protein / whey protein) of 2 to 20 to the casein protein.

“The active ingredient” means an ingredient required for exerting an effect of promoting muscle synthesis for a subject requiring immediate absorption of protein.

“Subjects requiring immediate absorption of protein” means subjects who need to promote muscle protein synthesis in order to repair muscle tissue and promote muscle growth, such as health promotion and exercise capacity For the purpose of improvement, it means a subject who exercises to promote muscle synthesis, prevent muscle loss, and promote muscle mass recovery. Here, the exercise is preferably strength training (resistance exercise), more preferably long-term strength training, for example, continuous, in that muscle synthesis is promoted by applying a certain load to the strength. Thus, the strength training is continued for 30 minutes to 5 hours at a frequency of 1 to 7 days in a week. Examples of strength training include dumbbell exercise, squat exercise, and machine training.

Examples of subjects who take the muscle synthesis promoter of the present invention include, for example, athletes, sports enthusiasts (athletes), those who need exercise to improve lifestyle-related diseases, and elderly people for health promotion. Examples include those who require exercise, or patients who suffer from a muscle injury disease (eg, sarcopenia) and who need to promote muscle synthesis through exercise. Further, the target may be an animal other than a human (domestic animals such as horses and cows, pets such as dogs and cats, appreciation animals kept in zoos, etc.).

The time when the muscle synthesis promoter of the present invention is ingested (administered) to a subject in need of immediate protein absorption may be before exercise, after exercise, or during exercise. Here, the time of intake is preferably after exercise, more preferably immediately after exercise. In terms of suppressing skeletal muscle degradation (catabolism) caused by exercise and promoting assimilation from amino acids to proteins, the intake period is preferably within 3 hours after the end of exercise, immediately after exercise. More preferably, within 2 hours after the end of exercise, more preferably within 1 hour after the end of exercise, and particularly preferably within 30 minutes after the end of exercise (Levenhagenhaet al. Am J physiol Endocrinol Metab (2001), 280, E982-E993).

Immediately effective muscle synthesis promoting effect equivalent to whey protein is the same level of protein absorption rate and absorption as observed when orally ingesting (administering) a protein mainly composed of whey protein. It means the same level of action as the accompanying muscle synthesis promoting action. The muscle synthesis promoting action includes an anabolic action of muscle protein from amino acids. Here, the muscle synthesis promoting action can be confirmed, for example, by measuring the triceps surae muscle synthesis rate (FSR) of the upper limb in the test group within 1 hour after administration of the test substance after the rat swimming test. it can. In other words, when the FSR of the test group is the same or higher than the FSR of the control group that orally ingested the protein mainly composed of whey protein, which is said to have an immediate effect of promoting muscle synthesis, Therefore, it can be judged that it is significant as a muscle synthesis promoting action (see Example 1 (2)). Further, the muscle synthesis promoting action includes a muscle protein degradation inhibiting action, that is, a catabolic preventing action from muscle protein to amino acid. Here, the degradation inhibitory action of muscle protein can be confirmed by measuring the concentration of 3 methylhistidine within 1 hour after administration of the test substance after, for example, a rat swimming test (Example 1). (See (3)).

"Protein amount" means the amount of protein as a polymer of amino acids contained in various proteins such as milk protein, whey protein or casein protein. “Protein amount ratio” means the ratio of the amount of protein as a polymer of amino acids contained in various proteins such as milk protein, whey protein, or casein protein. Here, the amount of protein may be calculated based on known information such as a food composition table, or may be calculated by measuring by a conventional method such as Kjeldahl method or Raleigh method. Actually, in the case of the Kjeldahl method, it can be calculated by measuring nitrogen contained in various proteins and multiplying the value by a nitrogen-protein conversion factor (usually 6.25).

The milk protein mixture used in the present invention is not particularly limited as long as it contains whey protein and casein protein in a specific protein amount ratio, but the whey protein and casein protein separated and purified separately are not specifically limited. It may be prepared by mixing so as to obtain a protein amount ratio, or a raw material (material) or food and drink that already contains whey protein and casein protein that have not been separated or purified separately in a specific protein amount ratio You may mix and prepare goods. At this time, the milk protein mixture used in the present invention does not require a separation step or purification step of whey protein or casein protein, and the production cost thereof is low, so that whey protein and casein protein are already specified. Prepared by mixing the raw materials and foods and drinks that are contained in the protein content ratio as the main components, and mixing the whey protein and casein protein purified separately as needed to achieve a specific protein content ratio. Is preferred.

Examples of whey protein separated and purified separately from casein protein include whey stock solutions (sweet whey, acid whey, etc.), concentrates, dried products (whey powder, etc.), frozen products, hydrolyzed products, etc. And reducing solutions thereof. Further, desalted whey, whey protein concentrate (WPC), purified whey protein (WPI), α-lactalbumin (α-La), β-lactoglobulin (β-Lg), immunoglobulin, lactoferrin, and the like, and These reducing solutions can be used. Casein protein separated and purified separately from whey protein includes, for example, casein isolate (natural cheese such as fresh cheese, sodium caseinate, calcium caseinate), its concentrate, its dried product, its A frozen material, a hydrolyzate thereof, a reducing solution thereof, and the like can be used. Furthermore, purified products such as α-casein, β-casein, and κ-casein, and reducing solutions thereof can be used. In addition, raw materials and foods and beverages that already contain whey protein and casein protein in a specific protein ratio include, for example, raw milk, pasteurized milk (such as milk), skim milk, component-adjusted milk, processed milk, and dairy products ( Concentrated milk, powdered milk, condensed milk, fermented milk (yogurt, etc.), lactic acid bacteria beverages, processed cheeses, ice creams, creams), milk protein concentrate (MPC), its concentrate, its dried product, its frozen product, its Hydrolysates and the like, and reducing solutions thereof can be used. At this time, you may obtain and use a commercial item for these, and may prepare and use it for themselves.

The milk protein mixture used in the present invention is composed of whey protein and casein protein having a specific protein amount ratio. Specifically, the protein amount ratio of casein protein to whey protein (casein protein / whey protein) is 2 to 20, preferably 2 to 10, more preferably 2 to 6, still more preferably 2 to 4, and particularly preferably 2 to 3.5. In the milk protein mixture used in the present invention, the protein ratio (casein protein / whey protein) between casein protein and whey protein is intentionally adjusted (designed), which is greatly different from the conventional technology. Yes.

The muscle synthesis promoter of the present invention has high heat resistance (thermal stability) and exhibits a heat resistance effect. Here, having a heat-resistant effect means that the protein does not solidify (aggregate) due to heat denaturation even when it is treated at a specific temperature. Specifically, it is a condition used in general processing of milk. This means that the protein is not heat denatured even if heat-sterilized by the method. More specifically, the muscle synthesis promoter of the present invention has a heat resistance effect. More specifically, it is heat sterilized (LTLT treatment: low temperature and long time heating) at 62 to 65 ° C. for 30 minutes, or As a method having a sterilizing effect of the same degree or more, for example, heat sterilization (UHT treatment: ultra-high temperature heating) at 120 to 150 ° C. for 1 to 30 seconds, or heat sterilization (HTST treatment: 72 ° C. for 15 seconds or more): This means that the protein is not easily heat denatured even if it is heated at high temperature for a short time or sterilized by heating at 75 ° C. or higher for 15 minutes or longer. At this time, the heat sterilization method includes a batch type in which the processing liquid is heated and held using a tank or the like, an indirect heating type using a plate type heat exchanger or a tube type heat exchanger, and a steam injection type heating device. And a known method such as a direct heating method using a steam infusion type heating device or a current heating method using a Joule heating device can be used.

According to one embodiment of the present invention, for example, in the muscle synthesis promoter of the present invention, the milk protein mixture is ingested (administered) so that the total protein amount is 0.13 g / kg body weight or more per meal, Specifically, the weight is 0.13 to 0.7 g / kg body weight, preferably 0.13 to 0.6 g / kg body weight, more preferably 0.13 to 0.5 g / kg body weight, still more preferably 0.00. It is ingested (administered) so as to be 15 to 0.4 g / kg body weight, particularly preferably 0.2 to 0.4 g / kg body weight. Here, the typical weight of the subject is estimated to be about 60 kg.

The term “one meal” means one time in a state requiring immediate absorption of protein. For example, in the muscle synthesis promoter of the present invention, a single meal is not taken at a time (at a time), but several times. May be taken separately. When the muscle synthesis promoter of the present invention is ingested (administered) to a subject in need of immediate protein absorption, the amino acid concentration in the bloodstream can be increased immediately when it is ingested in several degrees. In this respect, it is preferably within 2 hours after the end of exercise, more preferably within 1 hour after the end of exercise, more preferably within 30 minutes after the end of exercise, and particularly preferably the end of exercise. Within 5 minutes later.

The method for ingesting (administering) the muscle synthesis promoter of the present invention to a subject in need of immediate protein absorption can be appropriately selected from oral ingestion (oral administration), enteral administration, gastric fistula and the like depending on the subject and application. Although it can be selected, it is preferably taken orally.

The muscle synthesis promoter of the present invention can take any form as long as the effect is exerted, and examples thereof include a solid form, a liquid form, a gel form, a paste form, and a paste form. In the muscle synthesis promoter of the present invention, among solids, it may be powder, granule, capsule, block, In view of good processability, it is preferably a liquid. Here, for example, in the muscle synthesis promoter of the present invention, even if it contains moisture, it has heat resistance and can be sufficiently sterilized by heating. Therefore, for example, in the muscle synthesis promoter of the present invention, a long-term storage period can be set in a refrigerated state and at room temperature. In the muscle synthesis promoter of the present invention, the water content can be appropriately adjusted. Specifically, it may be 10 to 95% by mass, preferably 20 to 95% by mass, and more preferably. Is 30 to 90% by mass, more preferably 40 to 90% by mass. In the muscle synthesis promoter of the present invention, by adjusting the water content (or solid content) to a predetermined value, unlike conventional health drinks and sports drinks with high water content, muscle synthesis Can be effectively promoted.

According to another embodiment of the present invention, for example, the muscle synthesis promoter of the present invention comprises a unit package form per serving, and the milk protein mixture is added to the total amount of food intake in the unit package form. It is desirable that the amount of protein is adjusted to 8 g or more, specifically, 8 to 40 g, preferably 8 to 20 g, more preferably 9 to 20 g, still more preferably 9 to 15 g, particularly preferably 10 to 15 g. It adjusts so that it may become. Here, `` consisting of a unit packaging form per meal '' is a form in which the intake amount per meal is determined in advance, for example, as a food and drink that can be taken orally in a specific amount, not only general foods, It means forms such as beverages (drinks, etc.), health supplements, health functional foods, and supplements. In "unit packaging form per meal", for example, in the case of liquid beverages, gel-like, paste-like, paste-like jelly, powder-like, granule-like, capsule-like, block-like solid foods, etc. A specific amount (dose) can be defined in packaging containers such as cans, glass bottles (such as bottles), plastic containers (such as plastic bottles), packs, pouches, film containers, paper boxes, etc., or intake per serving (usage, dose) ) Is displayed on a packaging container, a homepage, etc., and the form which can prescribe | regulate specific quantity is mentioned.

According to another embodiment of the present invention, for example, when the form of the muscle synthesis promoter of the present invention is a so-called health drink, 8 to 40 g of the beverage in which the milk protein is suspended or dissolved is provided. The capacity (dose) of a single drink per meal is adjusted by the amount of water so that it is 100 to 1000 ml, and is placed in a packaging container such as a metal can, a glass bottle (such as a bottle), or a plastic container (such as a plastic bottle). (Filled) form. Alternatively, 8 to 40 g of the above-described milk protein suspension or dissolved beverage is displayed on a packaging container or homepage as the amount of intake (usage, dose) (for example, 100 to 1000 ml) per serving, and several servings (several (Batch) is a collective capacity, and is a form that is put (filled) in a large-capacity packaging container or the like.

As long as the muscle synthesis promoter of the present invention is manufactured so as to contain a predetermined milk protein mixture, its manufacturing process (manufacturing procedure) is not limited. For example, milk, ingredient-adjusted milk, processed milk, milk drink, etc. It can manufacture based on a manufacturing process. The muscle synthesis promoter of the present invention includes various components such as milk protein and milk fat with respect to raw milk (raw milk), sterilized milk, concentrated milk, powdered milk, and reduced milk according to a known manufacturing method (manufacturing method). It can be manufactured by blending (adjusting) the ingredients, or various ingredients such as milk protein and milk fat can be blended (adjusted) with component-adjusted milk and processed milk (such as low-fat milk and non-fat milk). ) Can also be manufactured.

According to one embodiment of the present invention, for example, the muscle synthesis promoter of the present invention can provide a composition comprising a muscle synthesis promoter. That is, the muscle synthesis promoter of the present invention can be used alone as it is, but as long as the muscle synthesis promotion function is exhibited, various compositions for oral intake (for oral administration) such as foods and drinks and pharmaceuticals. A composition (for example, a pharmaceutical composition or a food / beverage product composition) having an immediate effect of promoting muscle synthesis can also be obtained. Therefore, according to one embodiment of the present invention, a composition comprising the muscle synthesis promoter of the present invention is provided.

"Food and drink" (food and drink composition) is something other than a pharmaceutical product (pharmaceutical composition), as long as it can be taken orally, such as a solution, suspension, emulsion, powder, solid molded product, etc. Well, not particularly limited. Specific examples of food and drink include instant noodles, retort food, canned food, microwave food, instant soup and miso soup, freeze-dried food, etc .; soft drinks, fruit juice drinks, vegetable drinks, soy milk drinks, coffee Beverages such as beverages, tea beverages, powdered beverages, concentrated beverages, alcoholic beverages; flour products such as bread, pasta, noodles, cake mixes, bread crumbs; strawberries, caramel, chewing gum, chocolate, cookies, biscuits, cakes, pies, snacks , Crackers, Japanese confectionery, dessert confectionery, etc .; sauces, tomato processing seasonings, flavor seasonings, cooking mixes, sauces, dressings, soups, curry and stew ingredients; processed oils and fats, butter Oils and fats such as margarine and mayonnaise; milk drinks, fermented milk (yogurt, etc.), lactic acid bacteria drinks, natural tea 'S, process cheese, ice cream, dairy products such as cream; agricultural canning, jam, marmalade such, processed agricultural products such as serial; such as frozen food, and the like.

Foods and drinks include health foods, functional foods, dietary supplements, foods for specified health use, foods for the sick, infant formula, powdered milk for pregnant or lactating women, or rapid muscle synthesis. The thing of classification like the food-drinks which attached | subjected the indication that it is a thing used for promotion is also included.

“Pharmaceuticals” (pharmaceutical compositions) are those prepared as oral preparations or parenteral preparations in accordance with conventional methods, using additives that are acceptable for formulation. When the pharmaceutical is an oral preparation, it should be in the form of a solid preparation such as a tablet, powder, fine granule, granule, capsule, pill or sustained release, or a liquid preparation such as a solution, suspension or emulsion. Can take. Moreover, when a pharmaceutical is a parenteral formulation, it can take the form of an injection or a suppository. In terms of ease of ingestion (administration) to a patient, the pharmaceutical is preferably an oral preparation. Here, additives that are acceptable for formulation include, for example, excipients, stabilizers, preservatives, wetting agents, emulsifiers, lubricants, sweeteners, coloring agents, fragrances, buffers, antioxidants. Agents, pH adjusters and the like.

The muscle synthesis promoter of the present invention has a heat resistance effect. Therefore, in the composition in which the muscle synthesis promoter of the present invention is blended (added, mixed), the muscle synthesis promoter of the present invention has a heat-resistant effect, so that it can be preferably provided as a liquid product form. It can be provided as a product form for warm sales using a hot vendor or the like.

The composition of the muscle synthesis promoter of the present invention may be in any form as long as the effect is exerted, for example, solid, liquid, gel, Examples include pasty and pasty forms. The composition containing the muscle synthesis promoter of the present invention may be in the form of a solid, powder, granule, capsule, or block. However, it is preferably a liquid in terms of good processability.

According to one embodiment of the present invention, it is a food / beverage product obtained by blending (adding, mixing) and heat-sterilizing the muscle synthesis promoter of the present invention, comprising a unit packaging form per serving, and the unit packaging form Among them, it is desirable that the milk protein mixture is adjusted to a total protein amount of 8 to 40 g as a single meal intake, preferably 8 to 20 g, more preferably 9 to 20 g, still more preferably 9 to 15 g, particularly preferably. Food / beverage products and methods for producing the same are provided, which are provided with an effect of promoting muscle synthesis by immediate absorption of protein, adjusted to 10 to 15 g. Here, the operation (process) of “mixing (addition, mixing) and heat sterilization” to a predetermined component is not particularly limited as long as the operation is finally adjusted to a predetermined component and sterilized. Operation that mixes all necessary components and adjusts them to the prescribed components and then heat sterilizes them together, or operation that sterilizes the necessary individual components separately and mixes them to adjust them to the prescribed components Etc. Therefore, in the present invention, the order and number of times of this blending (addition, mixing) and heat sterilization are arbitrary.

At this time, the food / beverage product (the food / beverage product of the present invention) is preferably a food / beverage product packed in a packaging container, and more preferably a food / beverage product packed in a packaging container for heat sale. That is, since the food and drink have heat resistance, the protein will not be extremely denatured even if it is heated and sold. Therefore, in the food and drink composed only of the conventional whey protein or the whey protein as a main component, as a warm food and drink, it could not be provided outdoors in winter, etc., whereas in the food and drink, as a warm food and drink, It can be provided outdoors in winter (especially in cold conditions) and can be consumed (drinked) by consumers. Therefore, the practical use of the food-drinks with the effect of accelerating muscle synthesis corresponding to new needs can be expected. Here, in the food and drink for hot sale, for example, a hot sale beverage or a hot sale food stored in a hot vendor or hot water, and a necessary amount are put in a cooking utensil. In addition, heated beverages and heated foods heated by gas stoves, electric heating stoves, microwaves, and the like can be mentioned. In warm sales, it is generally stored at around 30-80 ° C or around 50-80 ° C.

According to a preferred embodiment of the present invention, the food or drink is a milk drink. This is because the food and drink have heat resistance, and thus the protein is not extremely denatured even if it is heat sterilized. Here, “milk beverage” means a beverage containing milk solids (a combination of nonfat milk solids and milk fat) at 3.0% by weight or more. For example, vitamins, minerals, oligosaccharides, lactose, fruit juice and the like can be blended in the milk beverage.

In the method for producing a food or drink according to the present invention, the production process (manufacturing procedure) is not limited as long as the food or drink is produced so as to contain a predetermined milk protein mixture (at a predetermined concentration). It can manufacture based on manufacturing processes, such as adjusted milk, processed milk, and a milk drink. The food / beverage products of this invention mix | blend various components, such as milk protein and milk fat, with respect to raw milk (raw milk), pasteurized milk, concentrated milk, powdered milk, reduced milk, for example according to a well-known manufacturing method (manufacturing method). It can be manufactured by (adjusting), and by blending (adjusting) various ingredients such as milk protein and milk fat into ingredient-adjusted milk and processed milk (such as low-fat milk and non-fat milk) It can also be manufactured.

According to another embodiment of the present invention, a milk protein mixture containing casein protein and whey protein, wherein the protein amount ratio of casein protein to whey protein (casein protein / whey protein) is 2 to 20, A milk beverage mixed and heat-sterilized with one or more selected from the group consisting of milk, concentrated milk, powdered milk and reduced milk, wherein the total protein content of the milk protein mixture is 8 g / 200 ml, preferably 8-20 g / 200 ml, more preferably 9 to 20 g / 200 ml, even more preferably 9 to 15 g / 200 ml, particularly preferably 10 to 15 g / 200 ml, and the milk fat amount is 8 g or less / 200 ml, preferably 0.1 to 8 g / 200 ml, more preferably 0.1-6 g / 200 ml, Provided is a packaged container-packed milk beverage that is preferably adjusted to 0.5 to 4 g / 200 ml, particularly preferably 0.5 to 3 g / 200 ml, and has an effect of promoting muscle synthesis by immediate protein absorption. .

According to yet another embodiment of the present invention, a milk protein mixture comprising casein protein and whey protein, wherein the protein amount ratio of casein protein to whey protein (casein protein / whey protein) is 2 to 20, A method for producing a packaged-packed milk beverage comprising a step of adding to one or more selected from the group consisting of raw milk, pasteurized milk, concentrated milk, powdered milk and reduced milk, and a heat sterilization step, wherein the milk protein The total protein amount of the mixture is adjusted to 8 g / 200 ml, preferably 8 to 20 g / 200 ml, more preferably 9 to 20 g / 200 ml, still more preferably 9 to 15 g / 200 ml, particularly preferably 10 to 15 g / 200 ml, and Milk fat content is 8g or less / 200ml, preferably 0.1-8g 200 ml, more preferably 0.1 to 6 g / 200 ml, further preferably 0.5 to 4 g / 200 ml, particularly preferably 0.5 to 3 g / 200 ml. Provided is a method for producing a packaged dairy beverage.

“Reduced milk” means liquid milk prepared by dispersing and dissolving milk raw materials such as powdered milk and concentrated milk in water. Examples of the milk raw material include whole milk powder, skim milk powder, whole fat concentrated milk, nonfat concentrated milk, butter, cream, and unfinished milk. Raw milk, pasteurized milk (such as milk), skimmed milk, component-prepared milk, If water is removed from processed milk etc., it will not restrict | limit, These may mix and use 1 or more types. In the milk beverage, aggregation due to heat denaturation of the whey protein is not observed (see Example 2), and therefore, the immediate protein absorption effect is maintained as in the muscle synthesis promoter of the present invention.

According to another embodiment of the present invention, a milk protein mixture comprising casein protein and whey protein, wherein the protein content ratio of casein protein to whey protein (casein protein / whey protein) is 2 to 20, There is provided a method of promoting muscle synthesis comprising ingesting a subject in need of absorption.

According to a preferred embodiment of the present invention, a milk protein mixture containing casein protein and whey protein having a protein amount ratio of casein protein to whey protein (casein protein / whey protein) of 2 to 20 is instantly absorbed by the protein. A method for promoting muscle synthesis (excluding medical practice for humans) comprising ingesting a subject in need thereof. Here, the “medical action for humans” means an action that requires a doctor or the like to ingest (administer) a medicine to a human. The muscle synthesis promoting method of the present invention can be carried out according to the contents described in the present specification for the muscle synthesis promoting agent of the present invention.

According to one preferred embodiment of the present invention, in the muscle synthesis promoting method of the present invention, the milk protein mixture is ingested (administered) so that the total protein amount is 0.13 g / kg body weight or more per meal. Specifically, the weight is 0.13-0.7 g / kg body weight, preferably 0.13-0.6 g / kg body weight, more preferably 0.13-0.5 g / kg body weight, still more preferably It can be ingested so as to be 0.15 to 0.4 g / kg body weight, particularly preferably 0.2 to 0.4 g / kg body weight.

According to one preferred embodiment of the present invention, in the method for promoting muscle synthesis of the present invention, the milk protein mixture can be ingested so that the total protein amount is 8 g or more per serving. It can be ingested so as to be ˜40 g, preferably 8 to 20 g, more preferably 9 to 20 g, still more preferably 9 to 15 g, particularly preferably 10 to 15 g.

According to one embodiment of the present invention, in the muscle synthesis promotion method of the present invention, the milk protein mixture can be ingested in a heated liquid form to a subject that needs immediate absorption of the protein.

According to one embodiment of the present invention, there is provided a muscle synthesis promoting agent comprising a milk protein mixture comprising casein protein and whey protein for promoting muscle synthesis of a subject in need of immediate protein absorption. Thus, the use of a muscle synthesis promoter having a protein amount ratio of casein protein to whey protein (casein protein / whey protein) of 2 to 20 is provided. The use of the muscle synthesis promoter of the present invention can be carried out according to the contents described herein for the muscle synthesis promoter of the present invention.

According to one embodiment of the present invention, there is provided a milk protein mixture comprising casein protein and whey protein for the manufacture of a food or beverage or pharmaceutical product for promoting muscle synthesis in a subject in need of immediate protein absorption. The use of a muscle synthesis promoter comprising a muscle synthesis promoter having a protein content ratio of casein protein to whey protein (casein protein / whey protein) of 2 to 20 is provided. The use of the muscle synthesis promoter of the present invention can be carried out according to the contents described herein for the muscle synthesis promoter of the present invention.

By the way, although specific experimental results and the like are omitted here, according to one embodiment of the present invention, not only an excellent muscle synthesis promoting action (muscle synthesis promoting effect) is exhibited but also an excellent performance is achieved. The body water retention action (body water retention effect) can also be exhibited. Therefore, in the present invention, it is possible to expect a specific effect that cannot be obtained with conventional pharmaceuticals and foods and drinks mainly composed of whey protein.

The present invention will be described in detail by the following examples, but the present invention is not limited thereto.

Example 1: Muscle synthesis promoting action by milk protein mixture (1) Rat swimming test SD male rats (obtained from CLEA Japan, Inc.) having an initial weight of 140 to 160 g were used as experimental animals. Rats were divided into 6 groups of 9 to 10 animals so that there was no difference in body weight between test groups (groups). All rats were individually housed in stainless steel cages at room temperature of 22 ± 2 ° C. with a light period of 7 to 19 o'clock and a dark period of 19 to 7 o'clock.

Using a solid feed (Oriental Yeast Co., Ltd.), rats were preliminarily raised for 3 to 4 days, and the rats were subjected to a swimming test according to the following protocol (the rats were exercised).

[Rat swimming test (exercise) protocol]
Day 1: Rats were subjected to 2 hours of swimming exercise, 45 minutes of rest, 2 hours of swimming exercise for a total of 4 hours of swimming without weight load. The diet after exercise (solid feed MF) was freely taken.
Day 2: The rats were subjected to 3 hours of swimming exercise, 45 minutes of rest, 3 hours of swimming exercise for a total of 6 hours without weight load. The diet after exercise (solid feed MF) was freely taken.
Day 3: Rats were subjected to a total of 6 hours of swimming exercise without weight load, 3 hours of swimming exercise, 45 minutes of rest, 3 hours of swimming exercise. As a diet after exercise, 5 g of a restricted diet (powder feed MF) was ingested.

After the above 3 days of exercise, the rats were subjected to a 2-hour swimming exercise without weight load. A milk beverage (milk protein solution) was prepared using milk protein and milliQ water so that the protein concentrations shown in Table 1 were obtained. After the exercise for 3 days, the rats were orally ingested milk drink and milliQ water at 24.24 ml / kg BW (test group 1-5 and comparative group 1). Here, the milk protein includes milk protein concentrate (MPC480, manufactured by Fontera, milk protein 77.2% by weight, milk fat content 1.2% by weight, lactose 9.2% by weight, ash content 7.1% by weight, Water (5.3 wt%) was used. This MPC480 is a mixture of casein protein and whey protein obtained by subjecting skim milk to membrane separation treatment (UF treatment, MF treatment, etc.), removing lactose and ash, and concentrating mainly on casein protein and whey protein. The form is a dry powder. And the protein amount ratio of MPC480 is casein protein: whey protein = about 8: 2 like raw milk (milk) (refer to Kunio Yamauchi and Kenkichi Yokoyama, Milk synthesis dictionary).

45 minutes after oral intake, 0.4 ml of deuterium labeled phenylalanine solution (prepared by dissolving 22.5 mg of deuterium labeled phenylalanine in 1 ml of physiological saline) per 200 g of body weight was injected from the tail vein. At 15 minutes after injection (ie, 60 minutes after oral ingestion), they were dissected under isoflurane anesthesia. After collecting all blood from the abdominal vena cava, the upper limb triceps (sample) was removed, washed with physiological saline, and immediately frozen with liquid nitrogen. The time of injection and the time of cryopreservation of the samples were recorded accurately. Samples were stored at −80 ° C. until analysis.

(2) Measurement of skeletal muscle synthesis rate (FSR) Based on the description in the previous literature (A. Kanda et al., Br J Nutr, Feb. 7, 1-7, 2013), skeletal muscle synthesis rate ( FSR) was measured. Specifically, using LC / MS / MS (manufactured by Waters), phenylalanine (Phe) and stable isotope labeled phenylalanine (present in skeletal muscle cells or free in skeletal muscle blood) Phe (Ring-D5)) was quantified. The measurement conditions are as follows. That is, apparatus: LC / MS / MS (manufactured by Waters), column: ACQUITY UPLC BEH C18 (manufactured by Waters), mobile phase: 0.05% TFA water / 0.05% TFA-containing acetonitrile, injection volume 3 μl, flow rate 0.3 ml / min, UV 215 nm.

Here, the skeletal muscle synthesis rate (FSR) was calculated by the following equation.
FSR (% / day) = (Eb × 100) / (Ea × t)
Ea: Enrichment of supernatant sample Eb: Enrichment of skeletal muscle sample t: Time from injection of injection solution to cryopreservation of sample (unit: days)
Enrichment = Phe (Ring−D5) / (Phe + Phe (Ring−D5))

The obtained numerical values were statistically processed to obtain an average value ± standard error. Specifically, a one-way analysis of variance was performed, followed by Tukey's multiple comparison test. The significance level was less than 5% (p <0.05). The result is shown in FIG. When there was a significant difference in the figure, different characters were attached. For example, it is shown that there is a significant difference between a group with a (comparison group 1 or the like) and a group with b (test group 3) or a group with c (test group 4 or the like). ing. Further, for example, it is shown that there is no significant difference between a group to which a (comparison group 1 or the like) or b (test group 3) is attached and a group to which ab is attached (test group 2).

At this time, it was confirmed that skeletal muscle synthesis was promoted in a dose-dependent manner by oral intake of milk protein.

In addition, it has been confirmed in the prior literature (A. Kanda et al., Br J Nutr, Feb. 7, 1-7, 2013) that the FSR is significantly reduced by two hours of swimming (Fig. 2). reference).

(3) Measurement of blood 3-methylhistidine (3MeHis) concentration Using LC / MS / MS (manufactured by Water), the sample was quantified for plasma 3MeHis concentration as one of the indicators of skeletal muscle degradation. The measurement conditions are as follows. That is, apparatus: LC / MS / MS (manufactured by Waters), column: ACQUITY UPLC BEH C18 (manufactured by Waters), mobile phase: 0.05% TFA water / 0.05% TFA-containing acetonitrile, injection volume 3 μl, flow rate 0.3 ml / min.

The obtained numerical values were statistically processed to obtain an average value ± standard error. Specifically, a one-way analysis of variance was performed, followed by Tukey's multiple comparison test. The significance level was less than 5%. The result is shown in FIG. When there was a significant difference in the figure, different characters were attached.

At this time, it was confirmed that oral intake of milk protein suppressed skeletal muscle degradation after exercise in a dose-dependent manner.

Example 2: Muscle synthesis promoting action by milk beverage containing milk protein (1) Preparation (production) of milk beverage containing milk protein
Raw milk (milk protein 3.3 wt%, milk fat 3.8 wt%, lactose 4.8 wt%, ash 0.7 wt%, moisture 87.4 wt%, obtained from Meiji Co., Ltd.) Protein was added and mixed to prepare raw milk so that the milk protein concentration shown in Table 2 was obtained. And according to the usual method for producing milk, the indirect heating method using a plate heat exchanger is sterilized by heating at 130 ° C. for 2 seconds, and then cooled to 10 ° C. or lower, so that the milk protein has a high concentration. (Milk test solution 6 and 7) was obtained. In addition, raw milk without milk protein (obtained from Meiji Co., Ltd.) was similarly heat sterilized and then cooled to 10 ° C. or lower to obtain milk (Test Section 8). Here, the milk protein added to the milk beverage includes milk protein concentrate (MPC480, manufactured by Fontera, milk protein 77.2% by weight, milk fat content 1.2% by weight, lactose 9.2% by weight, ash content 7 0.1 wt%, water 5.3 wt%). This MPC480 is a mixture of casein protein and whey protein obtained by subjecting skim milk to membrane separation treatment (UF treatment, MF treatment, etc.), removing lactose and ash, and concentrating mainly on casein protein and whey protein. The form is a dry powder. And the protein amount ratio of MPC480 is casein protein: whey protein = about 8: 2 like raw milk (milk) (refer to Kunio Yamauchi and Kenkichi Yokoyama, Milk synthesis dictionary).

In any of these milk drinks and milk obtained, no precipitates were observed due to protein aggregation (solidification) upon heating.

(2) Rat swimming test and measurement of skeletal muscle synthesis rate (FSR) 24.24 ml of raw milk (test group 8), milk drink (test group 6 and 7) and milliQ water (comparative group 1) shown in Table 2 A rat swimming test was conducted in the same manner as described in Example 1 except that the rat was orally ingested at / kg BW. Thereafter, the skeletal muscle synthesis rate (FSR) was measured (calculated).

As a result, the FSRs of test sections 6 and 7 were as high as 6.7% / day and 7.7% / day, respectively.

At this time, in the test sections 6 and 7, an immediate effect of promoting muscle synthesis was confirmed as in the case of taking whey protein alone orally.

It should be noted that the FSR is 8.8% / day when whey protein is taken orally at 3.11 g / kg BW after 2 hours of treadmill exercise as a protein amount. , J. Nutr. 137, 357-362 (2007)).

Claims (17)

1. A muscle synthesis promoter for a subject in need of immediate absorption of protein, comprising a milk protein mixture containing casein protein and whey protein as an active ingredient, wherein the protein amount ratio of casein protein to whey protein (casein (Protein / whey protein) is a muscle synthesis promoter having 2-20.
2. The muscle synthesis promoter according to claim 1, wherein the milk protein mixture is ingested so that a total protein amount is 0.13 to 0.7 g / kg body weight per meal.
3. The muscle synthesis promotion according to claim 1 or 2, comprising a unit packaging form per serving, wherein the milk protein mixture is adjusted to 8 to 40 g in terms of a total protein amount in a unit packaging form. Agent.
4. The muscle synthesis promoter according to any one of claims 1 to 3, wherein the subject requiring immediate protein absorption is a subject to exercise.
5. The muscle synthesis promoter according to claim 4, wherein the exercise is muscle training.
6. The muscle synthesis promoter according to any one of claims 1 to 5, which is in a liquid form.
7. A food and drink comprising the muscle synthesis promoter according to any one of claims 1 to 6 blended and heat-sterilized, comprising a unit packaging form per serving, wherein the milk protein is contained in the unit packaging form. A food and drink packaged in a packaged container, with the effect of promoting muscle synthesis by immediate absorption of protein, wherein the mixture is adjusted to 8 to 40 g in terms of the total protein intake.
8. A milk protein mixture containing casein protein and whey protein having a protein content ratio of casein protein to whey protein (casein protein / whey protein) of 2 to 20 is composed of raw milk, pasteurized milk, concentrated milk, powdered milk and reduced milk. A milk beverage obtained by mixing and heat-sterilizing with at least one selected from the group, wherein the milk protein mixture is adjusted to a total protein amount of 8 g / 200 ml and a milk fat amount of 8 g / 200 ml / ml. Packed container-packed dairy drink with added muscle synthesis-promoting action by immediate absorption.
9. A milk protein mixture containing casein protein and whey protein having a protein content ratio of casein protein to whey protein (casein protein / whey protein) of 2 to 20 is composed of raw milk, pasteurized milk, concentrated milk, powdered milk and reduced milk. A method for producing a packaging container-packed milk beverage comprising the step of adding to one or more selected from the group and the step of heat sterilization, wherein the milk protein mixture is 8 g / 200 ml in total protein and milk A method for producing a packaged-packed milk beverage, which is provided with an effect of promoting muscle synthesis by immediate protein absorption, wherein the fat amount is adjusted to 8 g or less / 200 ml.
10. A milk protein mixture containing casein protein and whey protein having a protein amount ratio of casein protein to whey protein (casein protein / whey protein) of 2 to 20 is to be ingested by a subject who needs immediate absorption of the protein. A method for promoting muscle synthesis, comprising medical treatment for humans.
11. A milk protein mixture containing casein protein and whey protein having a protein amount ratio of casein protein to whey protein (casein protein / whey protein) of 2 to 20 is to be ingested by a subject who needs immediate absorption of the protein. A method for promoting muscle synthesis, comprising:
12. The method for promoting muscle synthesis according to claim 10 or 11, wherein the milk protein mixture is ingested so that the total protein amount is 0.13 to 0.7 g / kg body weight per meal.
13. The method for promoting muscle synthesis according to claim 12, wherein the milk protein mixture is ingested so that the total protein amount of the milk protein mixture is 8 to 40 g per serving.
14. The method for promoting muscle synthesis according to any one of claims 10 to 13, comprising ingesting the milk protein mixture in a heated liquid form to a subject in need of immediate protein absorption.
15. The method for promoting muscle synthesis according to any one of claims 10 to 14, wherein a subject requiring immediate absorption of protein is a subject to exercise.
16. The method for promoting muscle synthesis according to claim 15, wherein the subject to exercise is a subject immediately after exercise.
17. The method for promoting muscle synthesis according to claim 15 or 16, wherein the exercise is muscle training.

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