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WO2014017851A3 - Novel mars and method for producing target protein using the same - Google Patents

Novel mars and method for producing target protein using the same Download PDF

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Publication number
WO2014017851A3
WO2014017851A3 PCT/KR2013/006679 KR2013006679W WO2014017851A3 WO 2014017851 A3 WO2014017851 A3 WO 2014017851A3 KR 2013006679 W KR2013006679 W KR 2013006679W WO 2014017851 A3 WO2014017851 A3 WO 2014017851A3
Authority
WO
WIPO (PCT)
Prior art keywords
same
target protein
producing target
mars
novel
Prior art date
Application number
PCT/KR2013/006679
Other languages
French (fr)
Other versions
WO2014017851A2 (en
Inventor
Sun Kyu Kim
Min Ji Ko
Yu Bin Choi
Eun A Kim
Dong In Kim
Kyung Duk Moon
Sang Kyung Park
Dong Heon Lee
Original Assignee
Hanwha Chemical Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hanwha Chemical Corporation filed Critical Hanwha Chemical Corporation
Publication of WO2014017851A2 publication Critical patent/WO2014017851A2/en
Publication of WO2014017851A3 publication Critical patent/WO2014017851A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to novel matrix attachment regions (MARs), a recombinant vector including the same, a transformant transformed with the recombinant vector, and a method for producing a target protein by culturing the transformant.
PCT/KR2013/006679 2012-07-27 2013-07-25 Novel mars and method for producing target protein using the same WO2014017851A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2012-0082822 2012-07-27
KR1020120082822A KR20140015999A (en) 2012-07-27 2012-07-27 Novel mars and method for producing target protein using the same

Publications (2)

Publication Number Publication Date
WO2014017851A2 WO2014017851A2 (en) 2014-01-30
WO2014017851A3 true WO2014017851A3 (en) 2014-05-08

Family

ID=49997929

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2013/006679 WO2014017851A2 (en) 2012-07-27 2013-07-25 Novel mars and method for producing target protein using the same

Country Status (2)

Country Link
KR (1) KR20140015999A (en)
WO (1) WO2014017851A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101599138B1 (en) * 2014-06-17 2016-03-03 한국생명공학연구원 Gene element enhancing expression of recombinant proteins in mammalian cells and uses thereof
KR101629564B1 (en) * 2014-09-30 2016-06-13 한국생명공학연구원 Recombinant vector for enhancing target gene-expression and use thereof
CN107058387B (en) * 2017-04-13 2019-09-06 新乡医学院 A bicistronic expression vector suitable for HEK293 cells and its preparation method, expression system, and application
CN110343718A (en) * 2018-04-03 2019-10-18 新乡医学院 A kind of fibrocyte expression vector of efficient stable, expression system and preparation method thereof, application

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20070118563A (en) * 2005-03-04 2007-12-17 주식회사 셀트리온 Animal cell expression vector in which 1 or more copies of MAR DNA is inserted at the 3 ′ end of the transcription termination region of a gene and a method of expressing a foreign gene using the same
KR20090053893A (en) * 2006-08-23 2009-05-28 셀렉시스 에스. 에이. Substrate attachment site (MARS) and its use to increase transcription
US20100226912A1 (en) * 2007-05-21 2010-09-09 Vivalis RECOMBINANT PROTEIN PRODUCTION IN AVIAN EBx® CELLS
WO2012046255A2 (en) * 2010-10-08 2012-04-12 Cadila Healthcare Limited Expression vector for high level expression of recombinant proteins

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20070118563A (en) * 2005-03-04 2007-12-17 주식회사 셀트리온 Animal cell expression vector in which 1 or more copies of MAR DNA is inserted at the 3 ′ end of the transcription termination region of a gene and a method of expressing a foreign gene using the same
KR20090053893A (en) * 2006-08-23 2009-05-28 셀렉시스 에스. 에이. Substrate attachment site (MARS) and its use to increase transcription
US20100226912A1 (en) * 2007-05-21 2010-09-09 Vivalis RECOMBINANT PROTEIN PRODUCTION IN AVIAN EBx® CELLS
WO2012046255A2 (en) * 2010-10-08 2012-04-12 Cadila Healthcare Limited Expression vector for high level expression of recombinant proteins

Also Published As

Publication number Publication date
WO2014017851A2 (en) 2014-01-30
KR20140015999A (en) 2014-02-07

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