WO2013155742A1 - Use of liuweidihuang preparation in preparing drugs for post-operative treatment of cardiac stenting - Google Patents

Abstract

Disclosed in the present invention is the use of a Liuweidihuang preparation in preparing drugs for post-operative treatment of cardiac stenting.

Description

 Use of Liuwei Dihuang preparation in preparing medicine for treating cardiac stent Technical field

 The invention relates to a new use of a traditional Chinese medicine preparation.

Background technique

Liuwei Dihuang Pill consists of rehmannia, hawthorn, yam, peony bark, diarrhea and medlar. It can be seen from the compatibility of drugs. It is based on the combination of Bushen Yin and tonifying the liver and spleen. The formula of Liuwei Dihuang Pill has the characteristics of three supplements and three diarrhea: Liuwei Dihuang Pills reuses the radix replenishing yin and tonifying the kidney, filling the marrow and purifying the marrow. It is a medicine for the monk, the mountain spleen nourishes the liver and kidney, and can refine the essence, taking the homologous meaning of liver and kidney, yam Replenishing spleen yin, can also solidify the kidney, a total of medicine, three drugs, kidney, spleen and yin and make up, is for three supplements, but the amount of rehmannia is the sum of hawthorn meat and yam, it is still mainly kidney . Alisma is damp and diarrhea, and can reduce the nourishment of rehmannia, dilute the spleen and wet, and help the health of yam, and diarrhea and diarrhea with diarrhea, help the real yin to regain its position, Danpi clear deficiencies Heat, and the warmth of the hawthorn, the three drugs are called Sanxie, all of which are adjuvants. Six flavors are combined, three supplements and three diarrhea, in which the amount of supplemental medicine is heavier than laxatives, which is mainly based on supplementation, liver, spleen and kidney, and yin and tonification. It is mainly used for kidney yin deficiency caused by weak waist and knees, dizziness, tinnitus, hand and foot fever, nocturnal emission and night sweats. After being verified by doctors of previous generations, the clinical curative effect is remarkable, and it has been passed down to this day. 'The ancestor of the prescription for yin.'

After cardiac stenting, patients need long-term anticoagulant drugs (such as clopidogrel), and also need to take statins, which are used to lower cholesterol, triglycerides and phospholipids, and increase high-density lipoprotein to prevent recurrence of myocardial infarction. At the same time, because patients with cardiac stents often have problems with low immunity, they also need to take drugs to improve their immune function. In this way, the patient is taking too much medicine, and because of the risk of muscle lysis of the statin, muscle soreness and weakness are more common side effects, so there is an urgent need to find an alternative medicine.

technical problem

After cardiac stenting, patients need long-term anticoagulant drugs (such as clopidogrel), and also need to take statins, which are used to lower cholesterol, triglycerides and phospholipids, and increase high-density lipoprotein to prevent recurrence of myocardial infarction. At the same time, because patients with cardiac stents often have problems with low immunity, they also need to take drugs to improve their immune function. In this way, the patient is taking too much medicine, and because of the risk of muscle lysis of the statin, muscle soreness and weakness are more common side effects, so there is an urgent need to find an alternative medicine.

Technical solution

 The present invention provides a novel use of a Liuwei Dihuang preparation.

 The invention relates to the use of a Liuwei Dihuang preparation for the preparation of a medicament for treating a cardiac stent.

Applicants found in the study that Liuwei Dihuang preparation has the functions of enhancing immunity, anti-wet, anti-hypoxia, anti-low temperature, lowering blood fat, lowering blood pressure and lowering blood sugar, as follows:

 Anti-wet, anti-low temperature, anti-hypoxia effect similar to ginseng;

 Effects on immune function: can activate cellular immunity, promote the induction of interferon by tonsil cells, and increase serum interferon levels;

 Dilation of blood vessels has a significant antihypertensive effect on arterial stenosis hypertension;

Improve blood rheology, reduce whole blood viscosity, plasma viscosity, fibrinogen, inhibit the oxygen free radicals in the infarcted heart, reduce infarct size, prevent coronary heart disease, myocardial infarction;

 The effect on blood lipids can significantly reduce cholesterol, triglycerides and phospholipids, increase high-density lipoprotein, increase HDL-C/TC The ratio promotes lipid metabolism, and long-term use has the effect of preventing atherosclerosis.

In a preferred embodiment, the Liuwei Dihuang preparation may be a pill, a capsule, a tablet, a soft capsule or a dropping pill, preferably a Liuwei Dihuang Pill, which is composed of a rehmannia, hawthorn, peony bark, yam, and Alisma. 6 composed of 茯苓 The extract of the traditional Chinese medicine is obtained as a raw material, and is prepared together with a carrier as a matrix of the dropping pills, and is characterized in that:

 1) The weight ratio of the extract of the 6 Chinese herbal medicines to the substrate is 1 : 1 to 8 ;

 2) The extract of the 6 Chinese herbal medicines is obtained by the method specified in the pharmacopoeia or ministerial standards;

 3) The dropping pill matrix has a weight average molecular weight of 6000 and a viscosity distribution of 13.6 to 14.5. Between the polyethylene glycol, the polyethylene glycol of the kind of polyethylene glycol has a weight content of 40 to 60%;

 4) accurately weigh the extract and the substrate according to the above ratio, put it in a heating container to heat it, melt it, stir it evenly, and then put it into the dropping machine, 75 ° C ~ 100 ° C, dripping into the condensing agent of 30 ° C ~ -8 ° C, the condensing agent is at least one of methyl silicone oil, liquid paraffin or vegetable oil, take out the dropping pills, wash and dry.

Beneficial effect

 The study found that the same PEG6000 (that is, polyethylene glycol with a weight average molecular weight of 6000), because it is The mixture of PEG1 ~ PEG + ∞ has a normal distribution. Among them, the PEG with a molecular weight of 6000 has the largest content, generally about 5%. The molecular weight of the invention is 6000. The weight of polyethylene glycol is 40-60% PEG6000 (that is, polyethylene glycol with a weight average molecular weight of 6000). The viscosity of this polyethylene glycol is between 13.6 and 14.5. Between the highest degree of polymerization, as a matrix of dropping pills, it is possible to obtain pills with high roundness, high hardness and a melting time controlled between 5 and 15 minutes, ensuring that the drug can be used quickly and efficiently. effect.

 When the Liuwei Dihuang preparation is used for the rehabilitation treatment of the patient after cardiac stenting, the dosage is the conventional dose of the Liuwei Dihuang preparation.

In view of the above functions of Liuwei Dihuang preparation, Liuwei Dihuang preparation has a promoting effect on the rehabilitation of patients after cardiac stenting, which on the one hand improves blood rheology, reduces whole blood viscosity, and significantly lowers cholesterol, triglyceride and phospholipid, and increases High-density lipoprotein can prevent the recurrence of myocardial infarction. On the other hand, it also increases the body's immunity and anti-wet effect, and improves the body's function, so that the drug can exert better therapeutic effect.

Embodiments of the invention

 The invention is further described in the following examples, without any limitation of the invention.

 Example 1

 1, take Rehmannia glutinosa 1408g, peony bark 528g, yam 704g, hawthorn 704g, Alisma 528g and 528 528g ;

 2, take 茯苓 350g pulverized into fine powder; Hawthorn plus ethanol reflux extraction twice, each time 1 Hour, filtered, the dregs are reserved, the filtrate is recovered from ethanol, concentrated to a thick paste; the peony bark is distilled by steam, and the distillate is added to 1 mol/L. The hydrochloric acid solution is crystallized and used; the distilled aqueous solution and the peony bark residue, the hawthorn dregs, the remaining barium, and the rehmannia root, the yam and the diarrhea are boiled three times, each time 1 Hour, filter, combine the filtrate, concentrate to a thick paste; add the above-mentioned fine powder and hawthorn thick paste, mix, low-temperature drying, pulverize into fine powder, add the above-mentioned peony bark extract, and mix to obtain Liuwei Dihuang powder;

 3. Add the extract of Liuwei Dihuang and the substrate in a ratio of 1:2, and mix well. The matrix is made of polyethylene glycol 6000. (weight average molecular weight), the viscosity of the polyethylene glycol is 14%, and the weight percentage of the polyethylene glycol having a molecular weight of 6000 is 50%;

 4. Heat the mixture to melt and stir evenly;

 5. Add the above materials to the dropping machine, keep the temperature at 80 ~ 85 °C, and then add 10 ~ 5 at the appropriate speed. In the methyl silicone oil of °C, it is taken out after shrinkage molding, the methyl silicone oil is removed, and it is dried.

 Example 2

 1, take Rehmannia glutinosa 1408g, peony bark 528g, yam 704g, hawthorn 704g, Alisma 528g and 528 528g ;

 2, the peony bark is distilled to extract the volatile oil, and the volatile oil of the peony bark is obtained. The aqueous solution is collected after 8 hours of distillation; the hawthorn is added 70%. The ethanol is refluxed and extracted twice, each time for 2 hours, the extract is combined, filtered, and the filtrate is reserved; the rehmannia root, yam, and diarrhea are boiled twice with water, and the first time added 8 times the amount of water for 2 hours, the second Add 6 times the amount of water to cook 1 hour, the decoction was combined, filtered, and the filtrate was combined with the above distilled aqueous solution, concentrated under reduced pressure to a clear density of 1.15 to 1.20, and allowed to cool, add ethanol to make the alcohol content up to 70%, stir well, static Set After 48 hours, the supernatant was combined with the above extract of Hawthorn, and the ethanol was recovered under reduced pressure until it was tasteless. After the mixture was boiled, it was immersed twice at 80 °C, and added with 6 times of water each time. Hour, the combined extracts were filtered, and the filtrate was concentrated under reduced pressure to a clear paste having a relative density of 1.15 to 1.20 (50 °C), combined with the above-mentioned stock solution, and concentrated to a relative density of 1.30 (50 ° C). The thick paste is dried under reduced pressure and pulverized into a fine powder to obtain an extract powder; the volatile oil of the peony bark and the extract powder are taken up;

 3, according to 1:8 The ratio of the extract of Liuwei Dihuang (composed of volatile oil and extract powder of peony bark) and the matrix were mixed and the matrix was selected from polyethylene glycol 6000 (weight average molecular weight). The viscosity of the polyethylene glycol was 14%. And the weight percentage of polyethylene glycol having a molecular weight of 6000 is 60%;

 4. Heat the mixture to melt and stir evenly;

 5, add the above materials to the dropping machine, keep the temperature at 95 ~ 100 °C, and then drop 0 ~ -8 at the appropriate speed In the methyl silicone oil of °C, it is taken out after shrinkage molding, the methyl silicone oil is removed, and it is dried.

 Table 1 Test data of Liuwei Dihuang Dropping Pills

project	Rounding rate	hardness	Time limit for melting (minutes)
Example 1	92	+++	<5
Example 2	92	+++	<5

 Note: The hardness is expressed in the attached table by placing the dropping pill on a glass plate and pressing it with a finger to observe the change in morphology. Among them, '+ 'It means that the tap is deformed, ' ++ ' means to force the deformation, ' +++ ' means that it is not deformed by force.

 Test example 1

 Forty SD rats were randomly divided into 4 groups: normal control group (I) and high fat model group (II). ), Liuwei Dihuang Dropping Pill Group (III, prepared in Example 1) and fenofibrate Control Group (IV); high fat model group, Liuwei Dihuang Dropping Pill Group and fenofibrate control group fat emulsion were administered by stomach Method modeling

 Method of administration and dosage: Liuwei Dihuang Dropping Pills Group 0.85g/Kg/d, administered intragastrically three times a day, the control group 0.40mg/Kg/d, administered intragastrically three times a day; for one month in a row;

 Effects of blood lipids and hemorheology on hyperlipidemia rats

 The experimental results are shown in Table 2.

 Table 2 Effects of blood lipids and hemorheology on hyperlipidemia rats

Group	TC	TG	HDL-C	LDL-C
Normal control group	2.95 ± 0.15	1.04 ± 0.08	2.02 ± 0.12	0.85 ± 0.05
High fat model group	3.88 ± 0.14*	1.69 ± 0.05**	1.65 ± 0.10**	1.95 ± 0.11**
Liuwei Dihuang Dropping Pills	3.15 ± 0.09**	1.05 ± 0.10**	2.12 ± 0.05**	1.55 ± 0.10*
Fenofibrate control group	3.32 ± 0.19*	1.12 ± 0.11**	1.50 ± 0.06	1.51 ± 0.15*

 * The high fat model group was compared with the normal control group P<0.05, ** the high fat model group was compared with the normal control group. P<0.01; * Liuwei Dihuang Pills group, fenofibrate control group and high fat model group compared P<0.05, ** Liuwei Dihuang Dropping Pill Group, fenofibrate control group and high fat model group compared P < 0.01.

 As a result, the Liuwei Dihuang Dropping Pill group can significantly reduce the levels of TC, TG, and LDL-C in the hyperlipidemia model ( P<0.05 or P<0.01), and in the aspect of increasing HDL-C, the Liuwei Dihuang Dropping Pill group was superior to the fenofibrate group.

 Effect of whole blood viscosity and plasma viscosity on hyperlipidemia rats

 The experimental results are shown in Table 3.

 Table 3 Effect of whole blood viscosity and plasma viscosity on hyperlipidemia rats

Group	Whole blood viscosity	Plasma viscosity
5S-1	200S-1
Normal control group	13.4 ± 0.2	6.0 ± 0.3	1.68 ± 0.01
High fat model group	17.6 ± 1.2*	6.4 ± 0.5	1.96 ± 0.05*
Liuwei Dihuang Dropping Pills	10.5 ± 1.0**	5.1 ± 0.6*	1.04 ± 0.38**
Fenofibrate control group	14.2 ± 0.5*	5.8 ± 0.3*	1.85 ± 0.15

 * Compared with the normal control group, the high fat model group was P<0.05; Compared with the high fat model group, the Liuwei Dihuang Drop Pill Group and the fenofibrate control group were compared with the high fat model group (P<0.05). ** The Liuwei Dihuang Dropping Pill Group and the fenofibrate control group were compared with the high fat model group. P<0.01 .

 The results showed that the Liuwei Dihuang Dropping Pill group significantly reduced whole blood viscosity and plasma viscosity.

 Test example 2

 1 Materials and methods

 1.1 sample: the Liuwei Dihuang Dropping Pill prepared in Example 2;

 1.2 Experimental animals: 80 female mice, weighing 18 to 22 g.

 1.3 Grouping and dose design: dose, 0.85g / Kg / d. The trials were randomly divided into 8 groups according to their body weight, each group 10 animals, each 2 groups is a large group, a total of four groups, respectively, a group of immunization: plaque, hemolysin determination; immune two groups: organ index, mouse peritoneal macrophage phagocytosis chicken red blood cell test; immunity Three groups: NK Activity, leaching test; four groups of immunization: DTH test.

 1.4 Instruments and reagents:

 1.4.1 Instruments: 8mm diameter puncher, micro blood coagulation test board, 2-16K universal centrifuge, MODEL680 Microplate reader, 96-well culture plate, Co-150CO2 incubator, spectrophotometer, inverted microscope, electronic balance, microscope.

 1.4.2 Reagents: Injectable ink, concanavalin (ConA), MTT, DNFB, acetone, sesame oil, SRBC, saline, chicken red blood cells, sheep red blood cells, methanol, Giemsa dye solution, YAC-1 cells, Hanks solution (pH 7.2-7.4), RPMI1640 Complete medium, lithium lactate, iodonitrotetrazolium chloride (INT), phenazine dimethyl sulfate (PMS), NAD, Tris-HCl buffer (pH 8.2), 1% NP40 , phenol blue, 1mol / L hydrochloric acid, acidic isopropanol and so on.

 1.5 Experimental data statistical method: experimental data to SPSS The software performs one-way analysis of variance. After the homogeneity test of variance, the experimental data of variance was analyzed by LSD method, and the experimental data of variance was statistically analyzed by Tambane method.

 1.6 Test method: After continuous gavage for 30 days, the following tests were performed separately.

 1.6.1 Delayed allergic reaction (DTH) test: The abdominal skin of mice is treated with an electric razor for a hair removal process, with a range of about 3 cm. *3cm, then evenly sensitize with 50μL of DNFB solution; after 5 days, apply evenly on the right side of the mouse with 10μl of DNFB solution for attack. twenty four After the hour, the mice were sacrificed by cervical dislocation. The left and right ears were cut out, and the ear piece with a diameter of 8 mm was removed by a puncher to calculate the weight difference between the left and right ears.

 1.6.2 Determination of antibody-producing cells and serum hemolysin: intraperitoneal injection of 2% (v/v) SRBC suspension per mouse 0.2 mL was used for immunization. After 5 days, the blood was collected from the eyeball for serum hemolysin detection, and the cervical spine of the animal was dissected and sacrificed. The spleen was taken for antibody-producing cell detection.

 1.6.2 .1 Antibody-producing cell assay: The cervical spine of the animal was dissected, the spleen was taken, ground with a glass homogenizer, and filtered through four layers of gauze, centrifuged (1000 rpm for 10 min), using Hank's Wash twice. Splenocytes were suspended in 8 mL of Hank's solution. The surface medium (1g agarose plus double distilled water 100mL) was heated and dissolved, and then placed in a 45 °C water bath to keep warm, with the same amount Mix the pH of 7.2 ~ 7.4, 2 times the concentration of Hank's solution, dispense small tubes, 0.5mL per tube, then add 50μL 10% SRBC, 25μL to the tube The spleen cell suspension was quickly mixed, poured onto a 6 cm plate of a brushed agar layer, incubated in a carbon dioxide incubator for 1.5 h, and then diluted with SA buffer (1:10). ), after continuing to incubate for 1.5 h, count the number of hemolysis plaques.

 1.6.2.2 Determination of serum hemolysin: The mice were removed from the eye and collected at 2000 r/min for 10 min. The serum was separated, and the serum was diluted with physiological saline. The serum of different dilutions was placed in a micro-hemagglutination plate, 100 μL per well, and then 100 μL of 0.5% (V/V) SRBC was added. The suspension was mixed, placed in a humidified flat plate, and incubated at 37 ° C for 3 h to observe the degree of hemagglutination.

 1.6.3 Mouse peritoneal macrophage phagocytosis chicken red blood cell experiment (half-in vivo method) and organ / body weight ratio: 20% per mouse intraperitoneal injection Chicken red cell suspension 1mL. At 30 min intervals, the animals were sacrificed by cervical dislocation, and the animals were fixed on the rat plate. The skin of the abdominal wall was cut in the middle, 2 mL of normal saline was injected through the abdominal cavity, and the rat plate was rotated for 1 min. . Then aspirate 1 mL of the peritoneal washing solution, and divide it into 2 pieces of glass slides, put them into an enamel box with wet gauze, and place them in a 37 °C incubator for 30 minutes. . After incubation, rinse in physiological saline to remove unpatched cells, air dry, fixed in 1 : 1 acetone methanol solution, stained with 4% (V/V ) Giemsa-phosphate buffer for 3 min. Rinse with distilled water and dry. The tablets were counted under an oil microscope and the phagocytosis rate and phagocytic index were calculated. The animals were dissected and the spleen and thymus were removed, and the blood was blotted with a filter paper and weighed.

 1.6.4 ConA-induced mouse lymphocyte transformation assay and mouse NK cell activity assay (lactate dehydrogenase LDH) Determination method: the mice were sacrificed by cervical dislocation, the spleen was aseptically taken, placed in a small dish containing appropriate amount of sterile Hanks solution, and the spleen was gently shredded with tweezers. The mesh was filtered to prepare a single cell suspension, and the cell suspension was divided into two parts for ConA-induced mouse lymphocyte transformation assay and mouse NK cell activity assay.

1.6.4 .1 ConA-induced mouse lymphocyte transformation assay: Wash the cell suspension twice with Hank's solution, centrifuge for 10 min (1000 r/min), then suspend the cells in 1 mL of complete medium, phenol blue The number of viable cells (95% or more) was counted by staining, and the cell concentration was adjusted to 3*10 ⁶ cells/mL. Each cell suspension was added to a 24-well culture plate in two wells, 1 mL per well, one well was added with 75 μl of ConA solution, and the other well was used as a control, and cultured in an incubator for 72 hours. 4 h before the end of the culture, 0.7 mL of the supernatant was aspirated per well, 0.7 mL of RPMI 1640 medium containing no calf serum was added, and MTT 50 μl/well was added thereto, and the culture was continued for 4 hours. After the completion of the culture, 1 mL of acidic isopropanol was added to each well, and the mixture was mixed by blowing to completely dissolve the purple crystals. Then, it was dispensed into a 96-well culture plate, and three parallel holes were made per well, and the optical density value was measured by a microplate reader at a wavelength of 570 nm.

1.6.4 .2 Determination of mouse NK cell activity (Lactate dehydrogenase LDH assay): The target cells were subcultured 24 hours before the test, washed three times with Hank's solution before use, and the cell concentration was adjusted with RPMI 1640 complete medium. 4* 10 ⁵ /mL. The spleen cell suspension was washed twice with Hank's solution and centrifuged for 10 min (1000 r/min) each time. Discard the supernatant and bounce the cytoplasm. Add 0.5 mL of sterilized water for 20 seconds. After lysing the red blood cells, add 0.5 mL of 2 times Hank's solution and 8 mL of Hank's solution, centrifuge for 10 min (1000 r/min), and resuspend with 1 mL of complete medium. 1% glacial acetic acid was diluted and counted. The number of viable cells was counted by phenol blue staining (should be above 95%), and the cell concentration was adjusted to 2*10 ⁷ cells/mL. Take 100 μL of target cells and effector cells (50:1 target ratio) and add to 96-well culture plates; target cells naturally release wells plus target cells and culture medium 100 μL each, target cells maximal release wells plus target cells and 1% 100 μL of NP40, each of which has three parallel wells, and cultured in a 37 ° C, 5% CO ₂ incubator for 4 hours, then centrifuge the plate (1500 r / min) for 5 min, and take 100 μL of supernatant from each well. In a 96-well culture plate, 100 μL of LDH substrate solution was added at the same time for 3 min, and 30 μL of 1 mol/L HCl was added to each well, and the optical density value (OD) was measured at 490 nm of the microplate reader to calculate the activity of NK cells.

 2 results:

 2.1 Determination of organ/body weight ratio: The thymus index of the drug-administered group was significantly higher than that of the control group, and the statistical difference was statistically significant ( P < 0.05), the results are shown in Table 4.

 Table 4 Determination of thymus index and spleen index ( eq \x\to(x) ± S )

Group	Number of animals (only)	Thymus index (%)	Spleen index (%)
Control group	10	0.28 ± 0.05	0.40 ± 0.10
G	10	0.39 ± 0.08*	0.36 ± 0.05

 * Compared with the control group P<0.05

 2.3 Determination of cellular immune function

 2.3.1 ConA-induced spleen lymphocyte transformation test in mice: sample administration group leaching OD The difference was significantly higher than that of the control group, and the statistical difference was statistically significant (P<0.01). The results are shown in Table 5.

 Table 5 ConA-induced mouse spleen lymphocyte transformation test (eq \x\to(x) ± S )

Group	Number of animals (only)	OD difference
Control group	10	0.072 ± 0.02
G	10	0.185 ± 0.05**

 ** Compared with the control group P<0.01

 2.3.2 Delayed allergic reaction test: The difference in swelling degree between the left and right ears of the drug-administered group was significantly higher than that of the control group, and the statistical difference was statistically significant ( P <0.01), the results are shown in Table 6.

 Table 6 DTH measurement results ( eq \x\to(x) ± S )

Group	Number of animals (only)	Left and right ear swelling degree difference ( g )
Control group	10	0.022 ± 0.05
G	10	0.031 ± 0.02**

 ** Compared with the control group P<0.01

 2.4 Determination of humoral immune function:

 2.4.1 Serum hemolysin test: The anti-volume volume of the mice in the drug-administered group was significantly higher than that in the control group, and the statistical difference was statistically significant ( P <0.01), the results are shown in Table 7.

 Table 7 Serum hemolysin test (eq \x\to(x) ± S )

Group	Number of animals (only)	Anti-volume
Control group	10	70.5 ± 22.2
G	10	102.2 ± 18.5* )

 ** Compared with the control group P<0.01

 2.4.2 Antibody-producing cell test: The number of hemolytic plaques in the drug-administered group was significantly higher than that in the control group, and the statistical difference was statistically significant ( P < 0.01), and the results are shown in Table 8.

 Table 8 Antibody-producing cell assay (eq \x\to(x) ± S )

Group	Number of animals (only)	Number of hemolytic plaques (*103 / whole spleen)
Control group	10	2.95 ± 0.55
G	10	5.68 ± 1.20**

 ) * Compared with the control group P<0.01

 2.5 Determination of macrophage function:

Mouse peritoneal macrophage phagocytosis chicken red blood cell test: the phagocytic index of erythrocyte phagocytosis in the peritoneal macrophages of the mice in the administration group was significantly higher than that in the control group, and the statistical difference was statistically significant (P<0.01). However, the phagocytic rate of erythrocyte phagocytosis in the peritoneal macrophages of the mice in the administration group was not statistically significant compared with the control group. The results are shown in Table 9.

 Table 9 Macrophage phagocytosis test ( eq \x\to(x) ± S )

Group	Number of animals (only)	Phagocytosis rate (%)	Phagocytic index
Control group	10	9.03 ± 1.08	0.13 ± 0.01
G	10	10.50 ± 1.20	0.16 ± 0.02**

 ** Compared with the control group P<0.01

 2.6 NK cell activity assay: The NK cell activity was significantly enhanced in the drug-administered group, and the difference was statistically significant compared with the control group ( P < 0.01), and the results are shown in Table 10.

 Table 10 NK cell activity assay (eq \x\to(x) ± S )

Group	Number of animals (only)	NK cell activity (%)
Control group	10	7.230 ± 4.18
G	10	16.50 ± 2.21**

 ** Compared with the control group P<0.01

The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and combinations thereof may be made without departing from the spirit and scope of the invention. Simplifications should all be equivalent replacements and are included in the scope of the present invention.

Claims (2)

1. Use of Liuwei Dihuang preparation in preparing medicine for treating cardiac stent

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2.  According to the claims Liuwei Dihuang 1 The use described in the invention is characterized in that: the preparation is a dropping pill, which is composed of cultivar, hawthorn, peony bark, yam, diarrhea and medlar. The extract of the traditional Chinese medicine is obtained as a raw material, and is prepared together with a carrier as a matrix of the dropping pills, and is characterized in that:

    1) The weight ratio of the extract powder of the 6-flavored Chinese medicine to the substrate is 1 : 1 to 8 ;

    2) The extract powder of the 6-flavored Chinese medicine is obtained by the method specified in the Pharmacopoeia or the ministerial standard;

   3) The dropping pill matrix is a polyethylene glycol having a weight average molecular weight of 6000 and a viscosity distribution between 13.6 and 14.5. In the polyethylene glycol, the molecular weight is 6000 polyethylene glycol content of 40 ~ 60%;

   4) Accurately weigh the extract and the substrate according to the above ratio, heat it in a heating container to melt, stir evenly, and then put it into the dropping machine at 75 °C ~ 100 °C, dripping into a condensing agent of 30 ° C to -8 ° C, the condensing agent is at least one of methyl silicone oil, liquid paraffin or vegetable oil, and the dropping pills are taken out, washed, and dried.