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WO2012096461A3 - 줄기세포의 부유배양용 조성물 - Google Patents

줄기세포의 부유배양용 조성물 Download PDF

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Publication number
WO2012096461A3
WO2012096461A3 PCT/KR2011/010159 KR2011010159W WO2012096461A3 WO 2012096461 A3 WO2012096461 A3 WO 2012096461A3 KR 2011010159 W KR2011010159 W KR 2011010159W WO 2012096461 A3 WO2012096461 A3 WO 2012096461A3
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WO
WIPO (PCT)
Prior art keywords
stem cells
composition
suspension culturing
culturing
mass
Prior art date
Application number
PCT/KR2011/010159
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English (en)
French (fr)
Other versions
WO2012096461A2 (ko
Inventor
이은주
김효수
Original Assignee
서울대학교병원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 서울대학교병원 filed Critical 서울대학교병원
Priority to US13/981,508 priority Critical patent/US9139811B2/en
Publication of WO2012096461A2 publication Critical patent/WO2012096461A2/ko
Publication of WO2012096461A3 publication Critical patent/WO2012096461A3/ko

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    • G—PHYSICS
    • G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
    • G09F—DISPLAYING; ADVERTISING; SIGNS; LABELS OR NAME-PLATES; SEALS
    • G09F9/00—Indicating arrangements for variable information in which the information is built-up on a support by selection or combination of individual elements
    • G09F9/30—Indicating arrangements for variable information in which the information is built-up on a support by selection or combination of individual elements in which the desired character or characters are formed by combining individual elements
    • G09F9/33—Indicating arrangements for variable information in which the information is built-up on a support by selection or combination of individual elements in which the desired character or characters are formed by combining individual elements being semiconductor devices, e.g. diodes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/4409—Non condensed pyridines; Hydrogenated derivatives thereof only substituted in position 4, e.g. isoniazid, iproniazid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • G—PHYSICS
    • G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
    • G09F—DISPLAYING; ADVERTISING; SIGNS; LABELS OR NAME-PLATES; SEALS
    • G09F9/00—Indicating arrangements for variable information in which the information is built-up on a support by selection or combination of individual elements
    • G09F9/30—Indicating arrangements for variable information in which the information is built-up on a support by selection or combination of individual elements in which the desired character or characters are formed by combining individual elements
    • G09F9/302—Indicating arrangements for variable information in which the information is built-up on a support by selection or combination of individual elements in which the desired character or characters are formed by combining individual elements characterised by the form or geometrical disposition of the individual elements
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N5/00—Details of television systems
    • H04N5/64—Constructional details of receivers, e.g. cabinets or dust covers
    • H04N5/645—Mounting of picture tube on chassis or in housing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • G—PHYSICS
    • G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
    • G09F—DISPLAYING; ADVERTISING; SIGNS; LABELS OR NAME-PLATES; SEALS
    • G09F7/00—Signs, name or number plates, letters, numerals, or symbols; Panels or boards
    • G09F7/18—Means for attaching signs, plates, panels, or boards to a supporting structure
    • G09F2007/1847—Brackets to grip the sign board

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Hematology (AREA)
  • Rheumatology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • General Physics & Mathematics (AREA)
  • Theoretical Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Multimedia (AREA)
  • Signal Processing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

본 발명은 부유배양 시 줄기세포의 세포사 억제용 조성물, 줄기세포의 대량 배양용 조성물 및 부유배양 시 줄기세포의 세포사 억제방법에 관한 것이다. 본 발명은 줄기세포, 특히 중배엽 줄기세포의 효율적인 단독 부유배양 또는 세포응집이 일어나지 않는 상황 하에서 효율적인 대량 부유배양에 유용하게 이용될 수 있다.
PCT/KR2011/010159 2011-01-14 2011-12-27 줄기세포의 부유배양용 조성물 WO2012096461A2 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US13/981,508 US9139811B2 (en) 2011-01-14 2011-12-27 Composition for suspension culturing of stem cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020110004016A KR101293637B1 (ko) 2011-01-14 2011-01-14 줄기세포의 부유배양용 조성물
KR10-2011-0004016 2011-01-14

Publications (2)

Publication Number Publication Date
WO2012096461A2 WO2012096461A2 (ko) 2012-07-19
WO2012096461A3 true WO2012096461A3 (ko) 2012-09-07

Family

ID=46507537

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2011/010159 WO2012096461A2 (ko) 2011-01-14 2011-12-27 줄기세포의 부유배양용 조성물

Country Status (3)

Country Link
US (1) US9139811B2 (ko)
KR (1) KR101293637B1 (ko)
WO (1) WO2012096461A2 (ko)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2837682B1 (en) * 2012-04-13 2017-09-20 Jeong Chan Ra Use of aspirin in composition for preventing stem cell disruption and aggregation
GB201220058D0 (en) * 2012-11-07 2012-12-19 Univ Manchester Cell differentiation
CN103789259A (zh) * 2014-02-10 2014-05-14 江苏霖峯细胞技术股份有限公司 自血液样品中同时分离间充质干细胞和造血干细胞的方法
KR102276424B1 (ko) 2014-10-06 2021-07-12 삼성전자주식회사 로-키나제 저해제를 포함하는 세포의 노화를 감소시키기 위한 조성물 및 그의 용도
DE102015100578A1 (de) * 2015-01-15 2016-07-21 Osram Opto Semiconductors Gmbh Bauelement und Verfahren zur Herstellung eines Bauelements
US11225644B2 (en) 2015-01-29 2022-01-18 Somar Corporation Cell culture method, cell aggregates, cell aggregation control agent, and medium
CN104830754B (zh) * 2015-05-08 2018-07-03 兰诺生物技术无锡有限公司 一种用于卵母细胞、皮肤成体干细胞培养的组合物及应用
KR101648915B1 (ko) 2016-05-16 2016-08-17 주식회사 디지레이 줄기세포의 응집방지 방법 및 그 조성물
JP6647545B2 (ja) * 2017-05-02 2020-02-14 剛士 田邊 医薬品組成物及び化粧品組成物
WO2019245074A1 (ko) * 2018-06-20 2019-12-26 주식회사 디오스템스 줄기세포의 생존율 및 분화율 향상 기능을 갖는 조성물
WO2021241781A1 (ko) * 2020-05-28 2021-12-02 부산대학교 산학협력단 줄기세포 배양용 재조합 파지-기반 용기 및 이의 용도
KR20230047879A (ko) 2021-10-01 2023-04-10 연세대학교 산학협력단 탈리도마이드계 화합물을 포함하는 세포 부유성 억제용 조성물 및 암 전이 억제용 약학 조성물

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008099662A (ja) * 2006-09-22 2008-05-01 Institute Of Physical & Chemical Research 幹細胞の培養方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2629652A1 (en) * 2007-04-24 2008-10-24 Yaojiong Wu Compositions for preventing or treating skin defects and methods of use thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008099662A (ja) * 2006-09-22 2008-05-01 Institute Of Physical & Chemical Research 幹細胞の培養方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
EMRE ET AL.: "The ROCK inhibitor Y-27632 improves recovery of human embryonic stem cells after fluorescence-activated cell sorting with multiple cell surface markers", PLOS ONE, vol. 5, no. ISSUE, 13 August 2010 (2010-08-13), pages 1 - 10 *
TAKEHARA ET AL.: "Rho-associated kinase inhibitor Y-27632 promotes survival of cynomolgus monkey embryonic stem cells", MOLECULAR HUMAN REPRODUCTION, vol. 14, no. 11, 21 October 2008 (2008-10-21), pages 627 - 634 *
WATANABE ET AL.: "A ROCK inhibitor permits survival of dissociated human embryonic stem cells", NATURE BIOTECHNOLOGY, vol. 25, no. 6, 27 May 2007 (2007-05-27), pages 681 - 686 *

Also Published As

Publication number Publication date
US9139811B2 (en) 2015-09-22
KR101293637B1 (ko) 2013-08-13
US20140011275A1 (en) 2014-01-09
KR20120082626A (ko) 2012-07-24
WO2012096461A2 (ko) 2012-07-19

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