WO2011016634A2 - Method for producing rice wine improved by the use of paprika, and rice wine produced thereby - Google Patents
Method for producing rice wine improved by the use of paprika, and rice wine produced thereby Download PDFInfo
- Publication number
- WO2011016634A2 WO2011016634A2 PCT/KR2010/004840 KR2010004840W WO2011016634A2 WO 2011016634 A2 WO2011016634 A2 WO 2011016634A2 KR 2010004840 W KR2010004840 W KR 2010004840W WO 2011016634 A2 WO2011016634 A2 WO 2011016634A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- paprika
- rice
- rice wine
- wine
- fermentation
- Prior art date
Links
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Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G3/00—Preparation of other alcoholic beverages
- C12G3/02—Preparation of other alcoholic beverages by fermentation
- C12G3/026—Preparation of other alcoholic beverages by fermentation with health-improving ingredients, e.g. flavonoids, flavones, polyphenols or polysaccharides, added before or during the fermentation stage; with flavouring ingredients added before or during the fermentation stage
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G3/00—Preparation of other alcoholic beverages
- C12G3/02—Preparation of other alcoholic beverages by fermentation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G3/00—Preparation of other alcoholic beverages
- C12G3/02—Preparation of other alcoholic beverages by fermentation
- C12G3/021—Preparation of other alcoholic beverages by fermentation of botanical family Poaceae, e.g. wheat, millet, sorghum, barley, rye, or corn
- C12G3/022—Preparation of other alcoholic beverages by fermentation of botanical family Poaceae, e.g. wheat, millet, sorghum, barley, rye, or corn of botanical genus Oryza, e.g. rice
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G3/00—Preparation of other alcoholic beverages
- C12G3/02—Preparation of other alcoholic beverages by fermentation
- C12G3/023—Preparation of other alcoholic beverages by fermentation of botanical family Solanaceae, e.g. potato
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G3/00—Preparation of other alcoholic beverages
- C12G3/04—Preparation of other alcoholic beverages by mixing, e.g. for preparation of liqueurs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12H—PASTEURISATION, STERILISATION, PRESERVATION, PURIFICATION, CLARIFICATION OR AGEING OF ALCOHOLIC BEVERAGES; METHODS FOR ALTERING THE ALCOHOL CONTENT OF FERMENTED SOLUTIONS OR ALCOHOLIC BEVERAGES
- C12H1/00—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages
- C12H1/22—Ageing or ripening by storing, e.g. lagering of beer
Definitions
- the present invention relates to a method for producing an improved rice wine using paprika and a rice wine produced therefrom.
- rice wine is a fermented food using microorganisms
- one of the biggest disadvantages of such fermented foods is taste.
- the present invention provides a method for producing paprika rice wine containing carotene pigment contained in paprika to enhance its functionality.
- Paprika is an annual plant belonging to Solanaceae, Capsicum and Annuum , and has six subspecies. Paprika is a Greek word that is commonly used in Europe. In Korea, bell type pepper (sweet pepper) that has no spicy taste is called paprika, and sweet pepper is called sweet pepper, bell pepper and French piment in English.
- Paprika was first grown in 1994 in a glass greenhouse on Jeju Island by Jedong Heungsan Co., Ltd. for use as an in-flight meal for the aircraft, and most of its production was exported because domestic consumption was not settled, but it was introduced as a major export item among fresh vegetables.
- the domestic consumption rate is also gradually increasing, showing its appearance in the mass media and consumer markets.
- Paprika contains carotenoid pigments such as capsanthine, ⁇ -cryptoxanthine, and zeaxanthine. It has little pungent taste, strong sweetness, and vitamins A and B 1
- C-rich alkaline tonic food is used as a food, salad, meat cooking spices.
- the present inventors have high moisture content and low shelf life, so that they can be dried for a long time, but not only the original color of spices, but also the two kinds of domestic paprika, special and fiesta varieties, in which loss of taste, aroma and nutrients occur, red color Varieties such as cupra, debra, funky, jubilee, son goku, magnifico, spider, ferrari, brittany, glubi, planty and yellow varieties kelly, confetti, malty, chelsea, clarity, orange boogie, president While making research on how to make functional paprika rice wine for the purpose of improving the utilization of paprika of Fellini, Rubato, and Narobi varieties, we focused on the technology of making improved paprika rice wine using microorganisms. Thus, the present invention has been completed.
- one object of the present invention is a high moisture content, loss of flavor, flavor, nutrients as well as the original color of spices during the storage process, and extremely poor storage properties, most of them are not used or processed almost at the time of production It is trying to standardize the method of making paprika rice wine, increasing the income of farmers, and making paprika rice wine and manufacturing method by making functional paprika rice wine using paprika, which is not being flooded and caused a price drop and waste of resources. .
- carotenoid-based pigments such as capsanthine, ⁇ -cryptoxanthine, zeaxanthine contained in paprika has an antioxidant and anti-cancer effect can be a great help for health promotion, artificial artificial to add to other liquor It is to provide rice wine with natural beautiful color without adding coloring.
- the present invention relates to a method for producing functional rice wine, and more specifically, an impurity step of fermenting rice; Joju manufacturing step of adding the yeast culture solution; Adding step of adding paprika and fermentation; After sterilization is characterized in that it comprises a low temperature aging step of aging in a cold storage.
- the imperial stage can be fermented for 45 to 50 hours at 25 to 28 °C, 80 to 90% humidity as a sugar substitute seed (cultivation bacteria of the bacterium Aspergillus oryzae ).
- the empire step may be added 0.2 to 0.3% by weight of the bacterium Aspergillus oryzae per 2 kg of steamed rice, preferably 0.25% by weight of the bacterium Aspergillus oryzae .
- the main production step may be adjusted to pH within the range of 3.0 to 3.2 by adding the yeast culture and using lactic acid and other organic acids.
- 150 to 250 mL of water and 10 to 20 mL of yeast culture solution may be added to 150 g of the prepared soup. Can be added.
- the feeding step may be added to the paprika and fermented in the 28 to 32 °C range.
- the feeding step is 2 kg of broth, 2 to 4 kg of steamed rice, 5 to 6 L of brewing water and 1 to 2 kg of crushed paprika puree, more preferably to 2 kg of soup, 3 kg of steamed rice , 5.6 L of brewing water and 1.32 kg of pulverized paprika puree can be added, and in some cases, the step of stirring is carried out every 2 to 3 hours while the sun is blocked and fermentation without changing the temperature at 28 to 32 ° C. Can be.
- the low temperature aging step after sterilization for 25 to 35 minutes at 60 to 70 °C can be low temperature aging for 25 to 35 days in a cold storage of 0 to 4 °C, preferably sterilized for 30 minutes at 65 °C 0 to 4 It can be matured for 30 days in a cold store at °C.
- It is prepared according to the method of the first aspect, and provides a functional rice wine having a content of free amino acid of 990.89 to 1013.47 mg / 100 g, pH 4.72 to 4.89, total acidity 0.52 to 0.58%, and circumference 15.2 to 15.8%.
- the rice wine prepared according to the method of the present invention not only has a higher content of free amino acids and lactic acid from rice than ordinary wines, but also contains vitamin C and carotenoid components having excellent anti-cancer and anti-oxidant effects, but has a water content.
- High and low shelf life can be dried and stored for a long time, but it is also possible to provide a health promoting effect by easily ingesting paprika, which is not only a spice original color but also a loss of taste, aroma and nutrients during processing.
- Figure 1 shows a flow chart illustrating a manufacturing process of functional paprika rice wine.
- FIG. 2 is a photograph of paprika rice wine prepared according to the flowchart of FIG. 1, (a) shows a special variety, and (b) shows a fiesta variety.
- Figure 3 shows a graph measuring the pH of the functional paprika rice wine.
- Figure 4 shows a graph measuring the total acidity of the functional paprika rice wine.
- Figure 5 shows a graph measuring the circumference of the functional paprika rice wine.
- Paprika used in the present invention is not particularly limited in its kind, for example, domestic paprika two varieties special, Fiesta varieties, as well as red varieties of cupra, debra, funky, jubilee, son goku, magni pico, spider , Ferrari, brittany, groovy, planty and yellow varieties kelly, confetti, malty, chelsea, clarity, orange varieties boogie, president, feliny, rubito, narobi varieties.
- the imperial step in order to produce a functional paprika rice wine, the imperial step is first performed.
- This imperial stage utilizes the existing brewing method, specifically, as can be seen in the flow chart shown in Figure 1, to wash rice (5 kg), the main ingredient of rice wine production to clean at room temperature (20 to 25 °C) 3 to 5
- the rice soaked in time is wrapped in mineral wood, and the water is completely removed for about 1 to 2 hours, and the rice is put in a steaming pot and cooked for 1 hour.
- the cooked rice was cooked in a soup room, rubbed and then crushed into chunks, and the mycelia increased by 0.25% by weight (5 g) of seed (cultured bacteria of Aspergillus oryza e, Chungmu fermented co., Ltd.) per 2 kg of cooked rice Mix very vigorously to penetrate deep inside the US. Evenly mixed cooked rice is fermented for 48 hours in a soup room with a temperature of 25 to 28 ° C. and a humidity of 80 to 90%.
- the master will then go through the manufacturing steps.
- the main hair is mixed by adding 200 mL of water and 15 mL of yeast ( Saccharomyces cerevisiae ) culture solution to 150 g of the soup prepared in the imperial stage, respectively, and the pH of the mixed solution using lactic acid to the pH range of 3.0 to 3.2. To prevent contamination of various bacteria.
- the mixture was put into a 2 L Erlenmeyer flask and shaken at 30 ° C. for 24 hours.
- the yeast culture solution is a culture solution obtained by inoculating yeast in a medium in which 0.5 g of yeast extract, 0.5 g of peptone, and 2 g of glucose are dissolved in 100 mL of distilled water.
- the rice wine prepared using paprika as also shown in the following examples, the content of free amino acids 990.89 to 1013.47 mg / 100 g, pH 4.72 to 4.89, the acidity provides a sour taste of food, but provides shelf life It is provided as a functional rice wine with a total acidity of 0.52 to 0.58%, and a weekly 15.2 to 15.8% corresponding to an important factor.
- Rice used in the present invention was purchased in a general mart, Aspergillus oryzae Purchased from Chungmu Fermentation Saccharomyces cerevisiae Is KCTC 7106, which was distributed at BRC, was used, and paprika was provided by Gyeongnam Agricultural Research and Development Institute.
- the jars were mixed with a hardwood cover and fermented at 28-32 ° C. without blocking of sunlight and fermentation at 28-32 ° C. for 10 days with stirring every 2-3 hours.
- the fermentation was confirmed by putting a match into the jar to confirm the fermentation completion.
- the fermentation is finished.
- sterilization at 65 ° C. for 30 minutes and then low temperature aging for 30 days in a low temperature storage at 0-4 ° C. To complete the product.
- a wine wine (Vina Ramvilla dry red wine, Capel Vinos S. Co., Spain) was prepared according to the method of preparing a general wine or commercially prepared wine.
- pH of two paprika rice wines (special varieties and Fiesta varieties) prepared in the above production example was measured using a pH meter (pH meter: ORION, U.S.A).
- the rice wine produced using the red paprika variety (special) was 4.89
- the rice wine manufactured with the yellow paprika variety (Fiesta) was 4.72. appear.
- the pH of the rice wine of the comparative example described above was 4.24, and the pH of the general wine of the comparative example was 3.51.
- the acidity may provide a sour taste of food, but it is an important factor for providing shelf life.
- the total acidity of two kinds of paprika rice wines prepared in the preparation example was titrated to 0.1 N-NaOH and the appropriate amount was converted to acetic acid. 4 is summarized.
- the total acidity of the functional paprika rice wine of the present invention was 0.52 and 0.58%, respectively, in red and yellow varieties.
- Two kinds of paprika rice wines (special varieties and Fiesta varieties) prepared in the above production example were distilled by alcohol distillation by alcohol distillation, and then measured using a spirit meter and summarized in FIG. 5.
- the degree of alcohol was 15.6 and 15.2%, respectively.
- the chromaticity of two kinds of paprika rice wines (special varieties and fiesta varieties) prepared in the above production example was measured using a colorimeter (CR-300, Minolta, Japan) and summarized in Table 1 below.
- the L value representing the brightness is 81.27 in rice wine prepared using a special variety, 88.63 in rice wine manufactured using a Fiesta variety, and the brightness is high in a sample prepared using the Fiesta variety. appear.
- the a value representing red rust was higher in the samples prepared with special varieties, and the b values for yellowish blue showed 29.84 and 29.86.
- the chromaticity of the rice wine prepared in the comparative example and the general wine of the comparative example was measured using a colorimeter (CR-300, Minolta, Japan), respectively.
- the L value of rice wine was 98.12, the a value was -0.61, the b value was 3.28, and the L value of ordinary wine was 32.79, the a value was 66.49, and the b value was 44.26.
- Inorganic component contents of two kinds of paprika rice wine (special varieties and Fiesta varieties) prepared in the preparation example were measured using an inductively coupled plasma spectrometer (Optima 5300DV, Perkin Elmer, USA) and summarized in Table 2 below.
- RF power was 1,300 W
- analysis pump flow rate was 1.5 mL / min
- gas flows were plasma: 15, auxiliary: 0.2
- nebulizer 0.8 L / min.
- the main inorganic components contained in the functional paprika rice wine was found to be potassium, sodium, phosphorus and calcium, and in the total inorganic component content in the rice wine prepared by using paprika of special and fiesta varieties. At 218.04 and 159.96 mg / 100 g, respectively, rice wines made with special varieties were slightly higher.
- the inorganic component content of the rice wine prepared in the comparative example and the control wine of the comparative example was measured using an inductively coupled plasma spectrometer (Optima 5300DV, Perkin Elmer, U.S.A.) as follows.
- the main inorganic ingredient of general rice wine was K. 64.49 mg / 100 g, which is the same as paprika wine, and the total content was 126.70 mg / 100 g, which was lower than paprika rice wine.
- K showed the highest content of 66.90 mg / 100 g, but the total inorganic content was 111.65 mg / 100 g, which was low in paprika rice wine.
- the amino acid content of two kinds of paprika rice wines (special varieties and Fiesta varieties) prepared in the above production example was measured using an amino acid autoanalyzer (Biochrom 30, Pharmacia Biotech Biochrom Co., Ltd., UK). Summarized in The column used for analysis was ultrapac 11 cation exchange resin (11 ⁇ m ⁇ 2 ⁇ m). The flow rate and buffer were 25 mL / hr and pH 3.20 ⁇ 10.0, respectively, and the column and reaction temperatures were 46 °C and It set to 88 degreeC, and the analysis time was 44 minutes.
- the major amino acids contained in functional paprika rice wine are aspartic acid, glutamic acid, proline and phenylalanine, and the total amino acid content of rice wine prepared using paprika of special and fiesta varieties is 1,013.47, respectively. And 990.89 mg / 100 g of rice wine produced as a special variety showed a slightly higher content.
- the inorganic component content of the rice wine prepared in the comparative example and the control wine of the general example was measured using an amino acid automatic analyzer as follows.
- the main amino acids of rice wine were also the same aspartic acid, glutamic acid, proline and phenylalanine as paprika rice wine, and the total amino acid content was 805.56 mg / 100 g.
- the major amino acids of wine were proline, serine and aspartic acid, and the total amino acid content was 130.96 mg / 100 g, which is different from paprika rice wine and rice wine.
- the sensory test of two paprika rice wines (special varieties and Fiesta varieties) manufactured in the above production example was carried out by 10 trained sensory test agents, and color, aroma, taste and overall taste degree of each sample were tested. It measured by the 9-point method and summarized in Table 4 below.
- the result of repeating the same sensory test for the rice wine prepared in the comparative example and the general wine of the control example is as follows.
- the overall preference of color, aroma and taste of rice without paprika was 6.15, which was lower than that of rice with paprika.
- the wines on the market have a distinctive color and aroma.
- the overall taste was 6.92, which was higher than that of paprika rice wine, but not significantly different.
- Rice wine prepared according to the method of the present invention not only has a high content of free amino acids and organic acids from rice, but also contains vitamin C and carotenoids, which are excellent in anticancer and antioxidant activity, and easily consume paprika as a processed food. By doing so, the health promoting effect can be exerted.
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Abstract
Description
본 발명은 파프리카를 이용하여 개량된 라이스 와인을 제조하는 방법 및 이로부터 제조된 라이스 와인에 관한 것으로, 구체적으로는 라이스 와인이 미생물을 이용한 발효 식품이므로 이같은 발효식품의 가장 큰 단점중의 하나인 맛의 불균형 및 재현성이 낮은 단점을 극복하고 표준화 공정을 제공함과 동시에 파프리카에 함유되어있는 카로틴 색소를 함유시켜 기능성을 증대시킨 파프리카 라이스 와인을 제조하는 방법을 제공한다. The present invention relates to a method for producing an improved rice wine using paprika and a rice wine produced therefrom. Specifically, since rice wine is a fermented food using microorganisms, one of the biggest disadvantages of such fermented foods is taste. To overcome the disadvantages of low imbalance and low reproducibility, and to provide a standardization process, the present invention provides a method for producing paprika rice wine containing carotene pigment contained in paprika to enhance its functionality.
파프리카는 가지과(Solanaceae), 고추속(Capsicum), 고추종(Annuum)에 속하는 한해살이 식물로 6개의 아종이 있으며, 파프리카란 말은 어원이 희랍어로 현재 유럽에서 모든 고추를 통칭하고 있다. 우리나라에서는 매운맛이 없는 bell type의 고추(단고추)를 파프리카라고 하고 있으며, 단고추는 영명으로 sweet pepper, bell pepper, 일본에서는 불어인 piment를 피망을 부르고 있다. Paprika is an annual plant belonging to Solanaceae, Capsicum and Annuum , and has six subspecies. Paprika is a Greek word that is commonly used in Europe. In Korea, bell type pepper (sweet pepper) that has no spicy taste is called paprika, and sweet pepper is called sweet pepper, bell pepper and French piment in English.
파프리카는 1994년 제동흥산(주)이 항공기 기내식용으로 이용하기 위하여 제주도의 유리온실에서 처음으로 재배하였으며, 국내 소비가 정착되지 않아 생산량의 대부분이 수출되고 있었으나, 신선채소 중 주요 수출 품목으로 소개되면서 매스컴과 소비시장에 모습을 보여 차츰 국내 소비율도 늘어나고 있는 추세이다. Paprika was first grown in 1994 in a glass greenhouse on Jeju Island by Jedong Heungsan Co., Ltd. for use as an in-flight meal for the aircraft, and most of its production was exported because domestic consumption was not settled, but it was introduced as a major export item among fresh vegetables. The domestic consumption rate is also gradually increasing, showing its appearance in the mass media and consumer markets.
품종별로는 빨간색, 주황색, 노랑색, 자주색 및 백색 등 다양한 색상을 가지고 있으며, 그 중 붉은색이 약 40%의 생산량을 차지하고 있다. 파프리카는 capsanthine, β-cryptoxanthine, zeaxanthine 등의 카로티노이드계 색소를 함유하고 있으며, 매운맛이 별로 없고, 단맛이 강하며, 비타민 A, B1 The varieties come in a variety of colors, including red, orange, yellow, purple and white, of which red is about 40%. Paprika contains carotenoid pigments such as capsanthine, β-cryptoxanthine, and zeaxanthine. It has little pungent taste, strong sweetness, and vitamins A and B 1
및 C가 풍부한 알칼리성 강장식품으로 음식, 샐러드, 고기 요리용 향신료로 많이 이용되고 있다. And C-rich alkaline tonic food is used as a food, salad, meat cooking spices.
지금까지 파프리카에 대한 연구는 착색단고추 재배현황 및 수출전망, 파프리카(단고추) 재배기술 및 품질관리 요령, 파프리카즙 첨가가 생면의 기호와 품질에 미치는 영향, 파프리카 추출물의 색소안정성과 ethoxyquin 및 잔류용매 검출, 파프리카즙을 첨가한 증편의 품질 특성 및 생 파프리카와 원적외선 건조 파프리카 분말의 지질 산화억제 효과, 한국산 파프리카의 품종별 화학성분과 같이 일부 연구에만 국한 되어 있고, 국내에서 생산되고 있는 파프리카를 이용한 가공품 개발에 관한 연구는 매우 미흡한 실정이다. Until now, the research on paprika has been carried out on the production status and export prospect of colored pepper, paprika cultivation technology and quality control method, the effect of adding paprika juice on the taste and quality of raw noodles, pigment stability of paprika extract, ethoxyquin and residue Detection of solvent, quality characteristics of Jeungpyun added paprika juice, lipid oxidation inhibitory effect of raw paprika and far-infrared dried paprika powder, and chemical composition of varieties of Korean paprika are limited to a few studies. There is very little research on the development of processed products.
종래의 파프리카를 이용하여 제조한 각종 가공품은 파프리카를 추출한 그 추출액을 단순히 첨가하여 빵, 떡볶이 떡 및 면에 직접 첨가하였는데 이는 파프리카 특유의 냄새를 가지고 있어 색깔은 좋아질 수 있으나 오히려 맛은 아무 것도 첨가하지 않은 것 보다 낮은 관능검사 결과를 보였다. Various processed products prepared using conventional paprika were added directly to bread, tteokbokki, and noodles by simply adding the extract of paprika, which has a characteristic smell of paprika, but the color can be improved, but taste is not added. The sensory results were lower than those without.
이에 본 발명자들은 수분함량이 높고, 저장성이 낮아 건조시켜 장기간 저장할 수 있지만 저장 과정 중에 향신료 본래의 색태뿐만 아니라 맛, 향, 영양소의 손실이 일어나는 국내산 파프리카 2품종인 스페셜 및 피에스타 품종뿐만 아니라, 붉은색 품종인 쿠프라, 데브라, 펑키, 쥬빌리, 손오공, 매그니피코, 스파이더, 페라리, 브리트니, 글루비, 플랜티 및 노란색 품종인 켈리, 컨페티, 몰티, 첼시, 클라리티, 주황색 품종인 부기, 프레지던트, 펠리니, 루바토, 나로비 품종의 파프리카에 대해서도 그 활용도를 향상시킬 목적으로 기능성 파프리카 라이스 와인을 제조하는 방법에 대하여 연구를 계속하던 중, 미생물을 활용하여 개량된 파프리카 라이스 와인을 제조하는 기술에 착안하여 본 발명을 완성하기에 이르렀다. The present inventors have high moisture content and low shelf life, so that they can be dried for a long time, but not only the original color of spices, but also the two kinds of domestic paprika, special and fiesta varieties, in which loss of taste, aroma and nutrients occur, red color Varieties such as cupra, debra, funky, jubilee, son goku, magnifico, spider, ferrari, brittany, glubi, planty and yellow varieties kelly, confetti, malty, chelsea, clarity, orange boogie, president While making research on how to make functional paprika rice wine for the purpose of improving the utilization of paprika of Fellini, Rubato, and Narobi varieties, we focused on the technology of making improved paprika rice wine using microorganisms. Thus, the present invention has been completed.
즉, 본 발명의 일 목적은 수분함량이 높고, 저장 과정 중에 향신료 본래의 색태뿐만 아니라 맛, 향, 영양소의 손실이 일어나며, 또한 저장성이 극히 불량하여 그 대부분이 생산시기에 맞추어 애용되거나 거의 가공되지 않은 상태로 홍수 출하되어 가격하락과 아울러 자원낭비도 초래되는 실정인 파프리카를 이용하여 기능성 파프리카 라이스 와인을 제조함으로써 파프리카의 활용범위 확대, 농가의 소득 증대 및 파프리카 라이스 와인 제조 및 제조방법을 표준화하려는데 있다. That is, one object of the present invention is a high moisture content, loss of flavor, flavor, nutrients as well as the original color of spices during the storage process, and extremely poor storage properties, most of them are not used or processed almost at the time of production It is trying to standardize the method of making paprika rice wine, increasing the income of farmers, and making paprika rice wine and manufacturing method by making functional paprika rice wine using paprika, which is not being flooded and caused a price drop and waste of resources. .
본 발명의 다른 목적은, 파프리카에 함유되어 있는 capsanthine, β-cryptoxanthine, zeaxanthine 등의 카로티노이드계 색소가 항산화 및 항암효과도 지니고 있어 건강증진에도 큰 도움을 줄 수 있으며, 기존의 다른 주류에 첨가하는 인공색소를 첨가하지 않아도 천연의 아름다운 색을 지닌 라이스 와인을 제공하려는데 있다.Another object of the present invention, carotenoid-based pigments, such as capsanthine, β-cryptoxanthine, zeaxanthine contained in paprika has an antioxidant and anti-cancer effect can be a great help for health promotion, artificial artificial to add to other liquor It is to provide rice wine with natural beautiful color without adding coloring.
본 발명의 제 1 견지에 의하면,According to the first aspect of the present invention,
본 발명은 기능성 라이스 와인의 제조방법에 관한 것으로, 보다 구체적으로 쌀을 발효시키는 제국단계; 효모 배양액을 첨가하는 주모 제조단계; 파프리카를 첨가하고 발효시키는 사입단계; 살균한 후 저온저장고에서 숙성시키는 저온 숙성 단계를 포함하는 것을 기술적 특징으로 한다.The present invention relates to a method for producing functional rice wine, and more specifically, an impurity step of fermenting rice; Joju manufacturing step of adding the yeast culture solution; Adding step of adding paprika and fermentation; After sterilization is characterized in that it comprises a low temperature aging step of aging in a cold storage.
상기 제국단계는 설탕 대용 종국(황국균 Aspergillus oryzae의 배양균)으로 쌀을 25 내지 28℃, 습도 80 내지 90%하에 45 내지 50시간 동안 발효시킬수 있다.The imperial stage can be fermented for 45 to 50 hours at 25 to 28 ℃, 80 to 90% humidity as a sugar substitute seed (cultivation bacteria of the bacterium Aspergillus oryzae ).
또한, 상기 제국단계는 증자미 2 kg에 대하여 0.2 내지 0.3 중량%의 황국균 Aspergillus oryzae를 첨가할 수 있으며, 바람직하게는 0.25 중량%의 황국균 Aspergillus oryzae를 첨가할 수 있다.In addition, the empire step may be added 0.2 to 0.3% by weight of the bacterium Aspergillus oryzae per 2 kg of steamed rice, preferably 0.25% by weight of the bacterium Aspergillus oryzae .
상기 주모 제조단계는 효모 배양액을 첨가하고 젖산 및 그 외의 유기산을 이용하여 pH를 3.0 내지 3.2 범위내로 조정할 수 있다.The main production step may be adjusted to pH within the range of 3.0 to 3.2 by adding the yeast culture and using lactic acid and other organic acids.
또한, 상기 주모 제조단계는, 제조된 국 150 g에 물 150 내지 250 mL 및 효모 배양액 10 내지 20 mL를 첨가할 수 있으며, 바람직하게는 제조된 국 150 g에 물 200 mL 및 효모 배양액 15 mL를 첨가할 수 있다.In addition, in the step of preparing the yeast, 150 to 250 mL of water and 10 to 20 mL of yeast culture solution may be added to 150 g of the prepared soup. Can be added.
상기 사입단계는 파프리카를 첨가하고 28 내지 32℃ 범위내에서 발효시킬 수 있다.The feeding step may be added to the paprika and fermented in the 28 to 32 ℃ range.
또한, 상기 사입단계는 국 2 kg에 주모, 증자미 2 내지 4 kg, 양조용수 5 내지 6 L 및 분쇄한 파프리카 퓌레 1 내지 2 kg을, 보다 바람직하게는 국 2 kg에 주모, 증자미 3 kg, 양조용수 5.6 L 및 분쇄한 파프리카 퓌레 1.32 kg을 첨가할 수 있으며, 경우에 따라서는 햇빛이 차단되고 28 내지 32℃에서 온도의 변화 없이 발효를 시키면서 2 내지 3시간 마다 교반하는 단계를 추가로 거칠 수 있다.In addition, the feeding step is 2 kg of broth, 2 to 4 kg of steamed rice, 5 to 6 L of brewing water and 1 to 2 kg of crushed paprika puree, more preferably to 2 kg of soup, 3 kg of steamed rice , 5.6 L of brewing water and 1.32 kg of pulverized paprika puree can be added, and in some cases, the step of stirring is carried out every 2 to 3 hours while the sun is blocked and fermentation without changing the temperature at 28 to 32 ° C. Can be.
상기 저온 숙성단계에서는 60 내지 70℃에서 25 내지 35분간 살균한 후 0 내지 4℃의 저온저장고에서 25 내지 35일 동안 저온숙성할 수 있으며, 바람직하게는 65℃에서 30분간 살균한 후 0 내지 4℃의 저온저장고에서 30일 동안 저온숙성 할 수 있다.In the low temperature aging step, after sterilization for 25 to 35 minutes at 60 to 70 ℃ can be low temperature aging for 25 to 35 days in a cold storage of 0 to 4 ℃, preferably sterilized for 30 minutes at 65
본 발명의 제 2견지에 따르면, According to the second aspect of the present invention,
상기 제 1견지의 방법에 따라 제조되며, 유리아미노산 함량 990.89 내지 1013.47 mg/100 g, pH 4.72 내지 4.89, 총 산도 0.52 내지 0.58%, 그리고 주정도 15.2 내지 15.8%인 기능성 라이스 와인을 제공한다. It is prepared according to the method of the first aspect, and provides a functional rice wine having a content of free amino acid of 990.89 to 1013.47 mg / 100 g, pH 4.72 to 4.89, total acidity 0.52 to 0.58%, and circumference 15.2 to 15.8%.
본 발명의 방법에 따라 제조된 라이스 와인에 따르면, 일반 포도주보다 쌀에서 나오는 유리아미노산 및 젖산 함량이 높을 뿐 아니라, 항암 및 항산화 작용에 뛰어난 효능을 갖는 비타민 C 및 카로테노이드 성분들을 함유하고 있으나 수분 함량이 높고 저장성이 낮아 건조시켜 장기간 저장할 수는 있지만 저장 과정도중 향신료 본래의 색택 뿐만 아니라 맛, 향, 영양소의 손실이 일어나는 파프리카를 가공 식품으로서 쉽게 섭취할 수 있도록 함으로써 건강 증진 효과 또한 제공할 수 있다.According to the rice wine prepared according to the method of the present invention, not only has a higher content of free amino acids and lactic acid from rice than ordinary wines, but also contains vitamin C and carotenoid components having excellent anti-cancer and anti-oxidant effects, but has a water content. High and low shelf life can be dried and stored for a long time, but it is also possible to provide a health promoting effect by easily ingesting paprika, which is not only a spice original color but also a loss of taste, aroma and nutrients during processing.
도 1은 기능성 파프리카 라이스 와인의 제조공정을 도시한 흐름도를 나타낸 것이다. Figure 1 shows a flow chart illustrating a manufacturing process of functional paprika rice wine.
도 2는 도 1의 흐름도에 따라 제조된 파프리카 라이스 와인의 사진으로서, (a)는 스페셜 품종을, 그리고 (b)는 피에스타 품종을 도시한 것이다. 2 is a photograph of paprika rice wine prepared according to the flowchart of FIG. 1, (a) shows a special variety, and (b) shows a fiesta variety.
도 3은 기능성 파프리카 라이스 와인의 pH를 측정한 그래프를 나타낸 것이다. Figure 3 shows a graph measuring the pH of the functional paprika rice wine.
도 4는 기능성 파프리카 라이스 와인의 총산도를 측정한 그래프를 나타낸 것이다. Figure 4 shows a graph measuring the total acidity of the functional paprika rice wine.
도 5는 기능성 파프리카 라이스 와인의 주정도를 측정한 그래프를 나타낸 것이다. Figure 5 shows a graph measuring the circumference of the functional paprika rice wine.
이하, 본 발명에 대하여 상세하게 설명한다. EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated in detail.
본 발명에서 사용되는 파프리카는 그 종류가 특별히 제한되지 않으며, 예를 들면, 국내산 파프리카 2품종 스페셜, 피에스타 품종 뿐만 아니라, 붉은색 품종인 쿠프라, 데브라, 펑키, 쥬빌리, 손오공, 매그니피코, 스파이더, 페라리, 브리트니, 글루비, 플랜티 및 노란색 품종인 켈리, 컨페티, 몰티, 첼시, 클라리티, 주황색 품종인 부기, 프레지던트, 펠리니, 루바토, 나로비 품종일 수 있다.Paprika used in the present invention is not particularly limited in its kind, for example, domestic paprika two varieties special, Fiesta varieties, as well as red varieties of cupra, debra, funky, jubilee, son goku, magni pico, spider , Ferrari, brittany, groovy, planty and yellow varieties kelly, confetti, malty, chelsea, clarity, orange varieties boogie, president, feliny, rubito, narobi varieties.
본 발명에서는 기능성 파프리카 라이스 와인을 제조하기 위하여, 우선 제국 단계를 수행하게 된다. 이같은 제국 단계는 기존 양조법을 활용한 것으로, 구체적으로는 도 1에 도시한 흐름도에서 확인할 수 있듯이, 라이스 와인 제조의 주재료인 쌀(5 kg)을 깨끗이 씻어 실온(20 내지 25℃)에서 3 내지 5시간 침지시킨 쌀을 광목에 싸서 1 내지 2시간 정도 물기를 완전히 제거하고 고두밥을 만들기 위해 쌀을 찜솥에 넣고 1시간 동안 증자한다. In the present invention, in order to produce a functional paprika rice wine, the imperial step is first performed. This imperial stage utilizes the existing brewing method, specifically, as can be seen in the flow chart shown in Figure 1, to wash rice (5 kg), the main ingredient of rice wine production to clean at room temperature (20 to 25 ℃) 3 to 5 The rice soaked in time is wrapped in mineral wood, and the water is completely removed for about 1 to 2 hours, and the rice is put in a steaming pot and cooked for 1 hour.
증자한 쌀(2 kg)을 광목위에 고루 펼친 다음 덩어리를 부수고, 품온이 32 내지 35℃로 냉각시킨 후 27 내지 28℃의 제국실안에 있는 작업대 위에 구상으로 쌓아 살균한 광목으로 덮어 1시간 정도 재워둔다. Spread the steamed rice (2 kg) evenly over the wood, crush the mass, cool the temperature to 32-35 ℃, stack it with a sterilized wood on the workbench in the empire room at 27-28 ℃, and soak for 1 hour. Put it.
재우기 공정을 거친 증자미를 국실안에서 헤치고 비벼서 덩어리를 부수고, 증자미 2 kg에 대하여 0.25 중량%(5 g)의 종국(황국균인 Aspergillus oryzae의 배양균, (주) 충무발효)을 균사가 증자미 내부 깊숙이 침입할 수 있도록 매우 격렬히 섞는다. 골고루 섞은 증자미를 온도 25 내지 28℃, 습도 80 내지 90%의 국실에서 48시간 동안 발효를 하면서 헤치기와 국상자 바꿔쌓기를 하면서 국을 제조(이하, '제국'이라 함)한다. The cooked rice was cooked in a soup room, rubbed and then crushed into chunks, and the mycelia increased by 0.25% by weight (5 g) of seed (cultured bacteria of Aspergillus oryza e, Chungmu fermented co., Ltd.) per 2 kg of cooked rice Mix very vigorously to penetrate deep inside the US. Evenly mixed cooked rice is fermented for 48 hours in a soup room with a temperature of 25 to 28 ° C. and a humidity of 80 to 90%.
그런 다음 주모 제조단계를 거치게 된다. 상기 주모는 제국 단계에서 제조된 국 150 g에 물 200 mL 및 효모(Saccharomyces cerevisiae) 배양액 15 mL를 각각 첨가하여 혼합하고, 첨가한 혼합액에 젖산을 이용하여 혼합액의 pH가 3.0 내지 3.2 범위가 되도록 pH를 조정함으로써 잡균의 오염을 방지케 한다. 상기 혼합액을 2 L 삼각플라스크에 넣어 30℃에서 24시간 동안 진탕배양을 한다. 여기서, 상기 효모 배양액은 효모추출물 0.5 g, 펩톤(peptone) 0.5 g 및 글루코스(glucose) 2 g을 증류수 100 mL에 녹인 배지에 효모를 접종하여 배양한 액이다.The master will then go through the manufacturing steps. The main hair is mixed by adding 200 mL of water and 15 mL of yeast ( Saccharomyces cerevisiae ) culture solution to 150 g of the soup prepared in the imperial stage, respectively, and the pH of the mixed solution using lactic acid to the pH range of 3.0 to 3.2. To prevent contamination of various bacteria. The mixture was put into a 2 L Erlenmeyer flask and shaken at 30 ° C. for 24 hours. Herein, the yeast culture solution is a culture solution obtained by inoculating yeast in a medium in which 0.5 g of yeast extract, 0.5 g of peptone, and 2 g of glucose are dissolved in 100 mL of distilled water.
그런 다음 주모 제조단계를 거친 국 2 kg, 주모, 증자미 3 kg, 양조용수 5.6 L 및 분쇄한 파프리카 퓌레 1.32 kg을 발효가 원활히 진행될 수 있도록 항아리에 차례대로 넣어 쌀이 부서지지 않을 정도로 혼합한다. 혼합한 항아리 입구를 광목으로 덮어주고 햇빛이 차단되고 온도의 변화가 없는 28 내지 32℃에서 발효를 시키면서 2 내지 3시간 마다 교반을 하며, 10일간 발효시킨다. 발효가 끝나면 쌀알이 항아리 표면위로 맑은 액이 생성되며, 발효완료 판정은 성냥으로 불을 붙여 항아리속에 넣어 불이 꺼지면 발효가 진행 중이고, 불이 그대로 있으면 발효가 끝난 것으로 판정한다. 발효가 완전히 끝난 라이스 와인에 저장성을 부여하기 위하여 65℃에서 30분간 살균한 후 0 내지 4℃의 저온저장고에서 30일 동안 저온숙성을 시켜 본 발명을 완성하였다.Then, 2 kg of broth, 3 kg of cooked rice, 3 kg of cooked rice, 5.6 L of brewed water and 1.32 kg of crushed paprika puree are placed in a jar in order to allow the fermentation to proceed smoothly. Cover the inlet of the mixed jars with a hardwood, stir every 2 to 3 hours while fermenting at 28 to 32 ℃ without blocking the sunlight and temperature changes, fermentation for 10 days. When the fermentation is finished, a grain of rice is produced on the surface of the jar, and the fermentation is judged as a match. The light is put into a jar and the fermentation is in progress. In order to impart a shelf life to the fermented rice wine completely, sterilization at 65 ℃ 30 minutes and then low temperature aging for 30 days in a low temperature storage of 0 to 4 ℃ to complete the present invention.
이상과 같이, 파프리카를 이용하여 제조된 라이스 와인은 하기 실시예에서도 규명된 바와 같이, 유리아미노산 함량 990.89 내지 1013.47 mg/100 g, pH 4.72 내지 4.89, 산도는 식품의 신맛을 제공하기도 하지만 저장성을 제공하는 중요한 인자에 해당하는 총 산도 0.52 내지 0.58%, 그리고 주정도 15.2 내지 15.8%인 기능성 라이스 와인으로서 제공된다.As described above, the rice wine prepared using paprika, as also shown in the following examples, the content of free amino acids 990.89 to 1013.47 mg / 100 g, pH 4.72 to 4.89, the acidity provides a sour taste of food, but provides shelf life It is provided as a functional rice wine with a total acidity of 0.52 to 0.58%, and a weekly 15.2 to 15.8% corresponding to an important factor.
이하, 본 발명을 실시예를 통하여 상세히 설명하도록 한다. 하기 실시예는 본 발명을 설명하기 위한 것이며, 이에 의해 본 발명의 범위가 제한되는 것은 아니다.Hereinafter, the present invention will be described in detail through examples. The following examples are intended to illustrate the invention, whereby the scope of the invention is not limited.
<실시예><Example>
재료material
본 발명에 사용된 쌀은 일반 마트에서 구입하였고, 종국인 Aspergillus oryzae는 충무발효에서 구입하였으며, 주모용 Saccharomyces cerevisiae는 생물자원센터에서 분양받은 KCTC 7106을 사용하였고, 파프리카는 품종이 입증된 경남 농업기술원에서 제공받아 사용하였다. Rice used in the present invention was purchased in a general mart,Aspergillus oryzaePurchased from Chungmu FermentationSaccharomyces cerevisiaeIs KCTC 7106, which was distributed at BRC, was used, and paprika was provided by Gyeongnam Agricultural Research and Development Institute.
파프리카 라이스 와인 제조Paprika Rice Wine Maker
<1단계: 제국단계> <Step 1: Empire Phase>
쌀 5 kg을 깨끗이 씻어 20-25℃의 실온하에 3-5시간 침지시킨 쌀을 광목에 싸서 1-2시간 정도 물기를 완전히 제거하고 고두밥을 만들기 위해 쌀을 찜솥에 넣고 1시간 동안 증자하였다. 5 kg of rice was washed thoroughly, and the rice soaked at room temperature of 20-25 ° C. for 3-5 hours was completely wrapped in ore and dried for about 1-2 hours, and the rice was put in a steaming pot and steamed for 1 hour.
증자한 쌀 2 kg을 광목위에 고루 펼친 다음 덩어리를 부수고, 품온이 32-35℃로 냉각시킨 후 27-28℃의 제국실안에 있는 작업대 위에 구상으로 쌓아 살균한 광목으로 덮어 1시간 정도 재워두었다. 2 kg of cooked rice was spread evenly over the wood, and then the mass was crushed, cooled to 32-35 ° C, and then sprinkled on a workbench in an imperial chamber at 27-28 ° C and covered for 1 hour.
이 같은 재우기 공정을 거친 증자미를 국실 안에서 헤치고 비벼서 덩어리를 부수고, 증자미 2 kg에 대하여 5g(증자미에 대해 0.25중량%)의 종국(황국균 Aspergillus oryzae를 키워놓은 것)을 균사가 증자미 내부 깊숙이 침입할 수 있도록 매우 격렬히 섞어주었다. 이같이 골고루 섞은 증자미를 온도 25-28℃, 습도 80-90%의 국실에서 48시간 동안 발효를 하면서 헤치기와 국상자 바꿔쌓기를 하면서 제국 공정을 수행하였다. Deeply cooked steamed rice cooked in this room through the cooking process, crushed and crushed into chunks, and 5g (0.25% by weight of cooked rice ) for 2 kg of cooked rice was grown (mycelia grows aspergillus oryzae ). Very vigorously mixed to invade. The evenly mixed steamed rice was fermented for 48 hours in a soup room with a temperature of 25-28 ° C and a humidity of 80-90%.
<2단계-주모 제조단계> <Step 2-Master Manufacturing Step>
상기 제1 단계에서 제조된 국 150 g에 물 200 mL, 효모(Saccharomyces cerevisiae, KCTC 7106)배양액 15 mL를 각각 첨가하였다. 첨가한 혼합액에 젖산을 이용하여 혼합액의 pH가 3.0-3.2 범위가 되도록 pH를 조정하였다. 얻어진 혼합액을 2 L 삼각플라스크에 넣어 30℃에서 24시간 동안 진탕배양하였다. To 150 g of the soup prepared in
<3단계 - 사입 단계> <Step 3-Insert Step>
상술한 방법으로 제조된 국 2 kg 및 주모와 증자미 3 kg, 양조용수 5.6 L 및 분쇄한 파프리카 퓌레(붉은색 special 품종과 노란색 fiesta 품종 각각에 대하여) 1.32 kg을 발효가 원활히 진행될 수 있도록 항아리에 차례대로 넣어 쌀이 부서지지 않을 정도로 혼합시켰다. 2 kg of the soup prepared by the above-described method, 3 kg of coarse and cooked rice, 5.6 L of brewing water and 1.32 kg of crushed paprika puree (for each of the red special varieties and the yellow fiesta varieties) were placed in a jar to facilitate the fermentation. Put in order to mix the rice so that it does not break.
혼합한 항아리 입구를 광목으로 덮어주고 햇빛이 차단되고 온도의 변화가 없는 28-32℃에서 발효를 시키면서 2-3시간 마다 교반하면서 총 10일간 발효시켰다. 쌀알이 항아리 표면위로 맑은 액이 생성되어 발효가 끝나면, 발효완료 판정을 위하여 성냥으로 불을 붙여 항아리 속에 넣어 확인하였다. The jars were mixed with a hardwood cover and fermented at 28-32 ° C. without blocking of sunlight and fermentation at 28-32 ° C. for 10 days with stirring every 2-3 hours. When the grains of rice were produced on the surface of the jar and the fermentation was completed, the fermentation was confirmed by putting a match into the jar to confirm the fermentation completion.
<4단계 - 저온 숙성단계> <Step 4-Low Temperature Ripening Step>
상기 제3 단계에서 성냥불이 그대로 있으면 발효가 끝난 것으로, 이같이 발효가 완전히 끝난 라이스 와인에 저장성을 부여하기 위하여 65℃에서 30분간 살균한 후 0-4℃의 저온저장고에서 30일 동안 저온숙성을 시켜 제품을 완성하였다. In the third step, if the matchstick is intact, the fermentation is finished. Thus, in order to impart shelf life to the finished fermented rice wine, sterilization at 65 ° C. for 30 minutes and then low temperature aging for 30 days in a low temperature storage at 0-4 ° C. To complete the product.
비교예 : 종래의 라이스 와인 제조Comparative Example: Conventional Rice Wine Production
본 발명에서 제조한 기능성 와인과 비교하기 위하여, 종래 라이스 와인의 제조방법에 따라 제국단계, 주모 제조단계, 사입단계 및 저온숙성단계를 거쳐 라이스 와인을 제조하였다. In order to compare with the functional wine prepared in the present invention, according to the conventional rice wine manufacturing rice wine was passed through the imperial stage, the main production stage, the stocking step and the low temperature aging step.
대조예 : 일반 포도주 와인 제조Comparative Example: General Wine Wine Production
본 발명에서 제조한 기능성 와인과 대조하기 위하여, 일반 포도주 와인의 제조방법에 따라 혹은 이미 제조되어 시판중인 포도주 와인(비나 람빌라 드라이 레드 와인, 카펠 비노스 에스에이 사, 스페인)을 준비하였다. In order to contrast with the functional wine produced in the present invention, a wine wine (Vina Ramvilla dry red wine, Capel Vinos S. Co., Spain) was prepared according to the method of preparing a general wine or commercially prepared wine.
실시예 1: 와인의 pH 대비Example 1 pH of Wine
상기 제조예에서 제조한 파프리카 라이스 와인 2종류(스페셜 품종, 피에스타 품종 각각)의 pH를 pH 미터(pH meter : ORION, U.S.A)를 사용하여 측정하였다. The pH of two paprika rice wines (special varieties and Fiesta varieties) prepared in the above production example was measured using a pH meter (pH meter: ORION, U.S.A).
본 발명의 기능성 파프리카 라이스 와인의 pH를 측정한 결과는 도 3에서 보듯이, 빨간색 파프리카 품종(스페셜)을 이용하여 제조한 라이스 와인는 4.89였으며, 노란색 파프리카 품종(피에스타)으로 제조한 라이스 와인은 4.72로 나타났다. As a result of measuring the pH of the functional paprika rice wine of the present invention, as shown in FIG. 3, the rice wine produced using the red paprika variety (special) was 4.89, and the rice wine manufactured with the yellow paprika variety (Fiesta) was 4.72. appear.
한편, 상술한 비교예의 라이스 와인의 pH를 측정한 결과는 4.24이었으며, 대조예의 일반 포도주 와인의 pH를 측정한 결과는 3.51이었다. On the other hand, the pH of the rice wine of the comparative example described above was 4.24, and the pH of the general wine of the comparative example was 3.51.
실시예 2: 와인의 총산도 대비Example 2: Comparison of Total Acidity of Wine
산도는 식품의 신맛을 제공하기도 하지만, 저장성을 제공하는 중요한 인자에 해당하는 것으로, 상기 제조예에서 제조한 파프리카 라이스 와인 2종류의 총산도를 0.1N-NaOH로 적정하고 적정량을 초산으로 환산한 결과를 도 4에 정리하였다. The acidity may provide a sour taste of food, but it is an important factor for providing shelf life. The total acidity of two kinds of paprika rice wines prepared in the preparation example was titrated to 0.1 N-NaOH and the appropriate amount was converted to acetic acid. 4 is summarized.
본 발명의 기능성 파프리카 라이스 와인의 총산도는 빨간색 및 노란색 품종 각각 0.52 및 0.58%였다. The total acidity of the functional paprika rice wine of the present invention was 0.52 and 0.58%, respectively, in red and yellow varieties.
한편, 상술한 비교예의 라이스 와인의 총 산도를 측정한 결과는 0.12%이었으며, 대조예의 일반 포도주 와인의 총 산도를 측정한 결과는 0.61%였다. On the other hand, the result of measuring the total acidity of the rice wine of the comparative example described above was 0.12%, the result of measuring the total acidity of the general wine wine of the control example was 0.61%.
실시예 3: 와인의 주정도 대비Example 3: Wine Contrast
상기 제조예에서 제조한 파프리카 라이스 와인 2종류(스페셜 품종, 피에스타 품종 각각)의 주정도를 알콜증류법으로 알콜을 증류시킨 다음 주정계를 이용하여 측정하고 도 5에 정리하였다. Two kinds of paprika rice wines (special varieties and Fiesta varieties) prepared in the above production example were distilled by alcohol distillation by alcohol distillation, and then measured using a spirit meter and summarized in FIG. 5.
본 발명의 2가지 라이스 와인에서 주정도는 각각 15.6와 15.2%로 나타났다. In the two rice wines of the present invention, the degree of alcohol was 15.6 and 15.2%, respectively.
한편, 상술한 비교예의 라이스 와인의 주정도를 측정한 결과는 14.1%이었으며, 대조예의 일반 포도주 와인의 주정도를 측정한 결과는 12.2%이었다. On the other hand, the result of measuring the circumference of the rice wine of the comparative example described above was 14.1%, the result of measuring the circumference of the general wine wine of the control example was 12.2%.
실시예 4: 색도 대비Example 4: Chromaticity Contrast
상기 제조예에서 제조한 파프리카 라이스 와인 2종류(스페셜 품종, 피에스타 품종 각각)의 색도를 색차계(CR-300, Minolta, Japan)를 이용하여 측정하고 하기표 1에 정리하였다. The chromaticity of two kinds of paprika rice wines (special varieties and fiesta varieties) prepared in the above production example was measured using a colorimeter (CR-300, Minolta, Japan) and summarized in Table 1 below.
표 1
상기 표 1에서 보듯이,밝기를 나타내는 L값은 스페셜 품종을 이용하여 제조한 라이스 와인에서는 81.27, 피에스타 품종을 이용하여 제조한 라이스 와인에서는 88.63로 나타나 밝기는 피에스타 품종을 이용하여 제조한 시료에서 높게 나타났다. 적녹도를 나타내는 a값은 스페셜 품종으로 제조한 시료에서 높았으며, 황청도는 나타내는 b값은 29.84 및 29.86으로 큰 차이를 보이지 않았다. As shown in Table 1, the L value representing the brightness is 81.27 in rice wine prepared using a special variety, 88.63 in rice wine manufactured using a Fiesta variety, and the brightness is high in a sample prepared using the Fiesta variety. appear. The a value representing red rust was higher in the samples prepared with special varieties, and the b values for yellowish blue showed 29.84 and 29.86.
한편, 상기 비교예에서 제조한 라이스 와인 및 대조예의 일반 포도주 와인의 색도를 각각 색차계(CR-300, Minolta, Japan)를 이용하여 측정한 결과는 다음과 같다. 라이스와인의 L값은 98.12, a값은 -0.61, b값은 3.28이었으며, 일반 포도주 와인의 L값은 32.79, a값은 66.49, b값은 44.26이었다. On the other hand, the chromaticity of the rice wine prepared in the comparative example and the general wine of the comparative example was measured using a colorimeter (CR-300, Minolta, Japan), respectively. The L value of rice wine was 98.12, the a value was -0.61, the b value was 3.28, and the L value of ordinary wine was 32.79, the a value was 66.49, and the b value was 44.26.
실시예 5: 와인의 무기성분 함량 대비Example 5 Inorganic Content of Wine
상기 제조예에서 제조한 파프리카 라이스 와인 2종류(스페셜 품종, 피에스타 품종 각각)의 무기 성분 함량을 유도결합플라즈마분광계(Optima 5300DV, Perkin Elmer, U.S.A.)를 이용하여 측정하고 하기 표 2에 정리하였으며, 분석조건 중 RF power는 1,300 W, analysis pump flow rate는 1.5 mL/min, gas flows는 plasma : 15, auxiliary : 0.2, nebulizer : 0.8 L/min으로 하여 분석하였다. Inorganic component contents of two kinds of paprika rice wine (special varieties and Fiesta varieties) prepared in the preparation example were measured using an inductively coupled plasma spectrometer (Optima 5300DV, Perkin Elmer, USA) and summarized in Table 2 below. RF power was 1,300 W, analysis pump flow rate was 1.5 mL / min, gas flows were plasma: 15, auxiliary: 0.2, nebulizer: 0.8 L / min.
표 2
상기 표 2에서 보듯이, 기능성 파프리카 라이스 와인에 함유되어 있는 주요 무기성분은 칼륨, 나트륨, 인 및 칼슘으로 나타났으며, 총 무기성분 함량에서는 스페셜 및 피에스타 품종의 파프리카를 이용하여 제조한 라이스 와인에서 각각 218.04 및 159.96 mg/100 g으로 스페셜 품종으로 제조한 라이스 와인에서 조금 높은 함량을 보였다. As shown in Table 2, the main inorganic components contained in the functional paprika rice wine was found to be potassium, sodium, phosphorus and calcium, and in the total inorganic component content in the rice wine prepared by using paprika of special and fiesta varieties. At 218.04 and 159.96 mg / 100 g, respectively, rice wines made with special varieties were slightly higher.
한편, 상기 비교예에서 제조한 라이스 와인 및 대조예의 일반 포도주 와인의 무기성분함량을 유도결합플라즈마분광계(Optima 5300DV, Perkin Elmer, U.S.A.)를 이용하여 측정한 결과는 다음과 같다. 일반 라이스 와인에 함유되어 있는 주요 무기성분은 파프리카 와인과 동일하게 K이 64.49 mg/100 g으로 높게 함유되어 있었으며, 총 함량은 126.70 mg/100 g으로 파프리카 라이스 와인보다 낮은 함량을 보였다. 또한 포도주의 경우도 K이 66.90 mg/100 g으로 가장 높은 함량을 보였으나 총 무기성분 함량은 111.65 mg/100 g으로 파프리카 라이스 와인과의 무기성분 함량이 낮았다. On the other hand, the inorganic component content of the rice wine prepared in the comparative example and the control wine of the comparative example was measured using an inductively coupled plasma spectrometer (Optima 5300DV, Perkin Elmer, U.S.A.) as follows. The main inorganic ingredient of general rice wine was K. 64.49 mg / 100 g, which is the same as paprika wine, and the total content was 126.70 mg / 100 g, which was lower than paprika rice wine. In the case of wine, K showed the highest content of 66.90 mg / 100 g, but the total inorganic content was 111.65 mg / 100 g, which was low in paprika rice wine.
실시예 6: 와인의 아미노산 함량 대비Example 6: Amino Acid Content of Wine
상기 제조예에서 제조한 파프리카 라이스 와인 2종류(스페셜 품종, 피에스타 품종 각각)의 아미노산 함량을 아미노산 자동분석기(Biochrom 30, Pharmacia Biotech Biochrom Co., Ltd., UK)를 사용하여 측정하였고, 하기 표 3에 정리하였다. 분석에 이용한 column은 ultrapac 11 cation exchange resin(11 μmㅁ2 μm)을 사용하였고, flow rate와 buffer는 각각 ninhydrin 25 mL/hr와 pH 3.20∼10.0으로 하였으며, column온도와 reaction온도는 각각 46℃와 88℃로 하였고, 분석시간은 44분이었다. The amino acid content of two kinds of paprika rice wines (special varieties and Fiesta varieties) prepared in the above production example was measured using an amino acid autoanalyzer (Biochrom 30, Pharmacia Biotech Biochrom Co., Ltd., UK). Summarized in The column used for analysis was ultrapac 11 cation exchange resin (11 μm ㅁ 2 μm). The flow rate and buffer were 25 mL / hr and pH 3.20 ~ 10.0, respectively, and the column and reaction temperatures were 46 ℃ and It set to 88 degreeC, and the analysis time was 44 minutes.
하기 표 3에서 보듯이, 기능성 파프리카 라이스 와인에 함유되어 있는 주요 아미노산으로는 aspartic acid, glutamic acid, proline 및 phenylalanine이며, 스페셜 및 피에스타 품종의 파프리카를 이용하여 제조한 라이스 와인의 총아미노산 함량은 각각 1,013.47 및 990.89 mg/100 g으로 스페셜 품종으로 제조한 라이스 와인에서 조금 높은 함량을 보였다. As shown in Table 3, the major amino acids contained in functional paprika rice wine are aspartic acid, glutamic acid, proline and phenylalanine, and the total amino acid content of rice wine prepared using paprika of special and fiesta varieties is 1,013.47, respectively. And 990.89 mg / 100 g of rice wine produced as a special variety showed a slightly higher content.
한편, 상기 비교예에서 제조한 라이스 와인 및 대조예의 일반 포도주 와인의 무기성분함량을 아미노산 자동분석기를 이용하여 측정한 결과는 다음과 같다. 일반 라이스와인의 주요 아미노산은 역시 파프리카 라이스 와인과 동일한 aspartic acid, glutamic acid, proline 및 phenylalanine이었으며, 총 아미노산 함량은 805.56 mg/100 g였다. 또한 포도주의 주요 아미노산은 proline, serine 및 aspartic acid였으며, 총 아미노산 함량은 130.96 mg/100 g으로 파프리카 라이스 와인과 라이스 와인과 많은 함량 차이를 보였다. On the other hand, the inorganic component content of the rice wine prepared in the comparative example and the control wine of the general example was measured using an amino acid automatic analyzer as follows. The main amino acids of rice wine were also the same aspartic acid, glutamic acid, proline and phenylalanine as paprika rice wine, and the total amino acid content was 805.56 mg / 100 g. The major amino acids of wine were proline, serine and aspartic acid, and the total amino acid content was 130.96 mg / 100 g, which is different from paprika rice wine and rice wine.
표 3
실시예 7: 와인의 관능검사 결과 대비Example 7 Comparison of Sensory Test Results of Wine
상기 제조예에서 제조한 파프리카 라이스 와인 2종류(스페셜 품종, 피에스타 품종 각각)에 대한 관능 검사를 훈련된 10명의 관능검사 요원에 의해 실시하였으며, 각 시료에 대하여 색, 향, 맛 및 종합적인 기호도를 9점법으로 측정하고 하기 표 4에 정리하였다.The sensory test of two paprika rice wines (special varieties and Fiesta varieties) manufactured in the above production example was carried out by 10 trained sensory test agents, and color, aroma, taste and overall taste degree of each sample were tested. It measured by the 9-point method and summarized in Table 4 below.
하기 표 4에서 보듯이, 색, 향기 및 맛에서는 큰 유의적인 차이는 보이지 않았으나 전체적인 기호도에서 피에스타 품종을 이용하여 제조한 라이스 와인 6.36보다, 스페셜 품종의 파프리카를 첨가하여 제조한 라이스 와인에서 6.85로 높은 기호도를 보였다. As shown in Table 4, there was no significant difference in color, aroma and taste, but the overall preference was higher than the rice wine 6.36 prepared using the Fiesta varieties, the rice wine prepared with the addition of special varieties of paprika was 6.85 higher Showed sign too.
표 4
1) 1∼9 등급 : 1, 매우 나쁘다.; 5, 보통이다.; 9; 매우 좋다. 1) Grades 1-9: 1, very bad; 5, normal; 9; very good.
한편, 상기 비교예에서 제조한 라이스 와인 및 대조예의 일반 포도주 와인에 대하여 동일한 관능검사를 반복한 결과는 다음과 같다. 파프리카를 첨가하지 않은 일반 라이스와인의 색, 향기, 맛을 종합한 종합적인 기호도에서 6.15로 파프리카를 첨가한 라이스와인보다 낮은 기호도를 보였으나, 시중에 판매하는 포도주와인은 포도주 특유의 색과 포도주에 길들여져 있는 입맛에 의하여 종합적인 기호도가 6.92로 파프리카 라이스 와인보다 높은 기호도를 보였으나 큰 차이를 보이지는 않았다. On the other hand, the result of repeating the same sensory test for the rice wine prepared in the comparative example and the general wine of the control example is as follows. The overall preference of color, aroma and taste of rice without paprika was 6.15, which was lower than that of rice with paprika. However, the wines on the market have a distinctive color and aroma. The overall taste was 6.92, which was higher than that of paprika rice wine, but not significantly different.
본 발명의 방법에 따라 제조된 라이스 와인은 쌀에서 나오는 유리아미노산 및 유기산 함량이 높을 뿐 아니라, 항암 및 항산화 작용에 뛰어난 효능을 갖는 비타민 C 및 카로테노이드 성분들을 함유하고 있으며 파프리카를 가공 식품으로서 쉽게 섭취할 수 있도록 함으로써 건강 증진 효과를 발휘할 수 있다. Rice wine prepared according to the method of the present invention not only has a high content of free amino acids and organic acids from rice, but also contains vitamin C and carotenoids, which are excellent in anticancer and antioxidant activity, and easily consume paprika as a processed food. By doing so, the health promoting effect can be exerted.
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