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WO2011009975A3 - Método para la detección e identificación de especies de la familia engraulidae - Google Patents

Método para la detección e identificación de especies de la familia engraulidae Download PDF

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Publication number
WO2011009975A3
WO2011009975A3 PCT/ES2010/000314 ES2010000314W WO2011009975A3 WO 2011009975 A3 WO2011009975 A3 WO 2011009975A3 ES 2010000314 W ES2010000314 W ES 2010000314W WO 2011009975 A3 WO2011009975 A3 WO 2011009975A3
Authority
WO
WIPO (PCT)
Prior art keywords
seq
engraulidae
family
species
detecting
Prior art date
Application number
PCT/ES2010/000314
Other languages
English (en)
French (fr)
Other versions
WO2011009975A2 (es
Inventor
Laureana REBORDINOS GONZÁLEZ
Hicham Chairi
Original Assignee
Universidad De Cádiz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad De Cádiz filed Critical Universidad De Cádiz
Publication of WO2011009975A2 publication Critical patent/WO2011009975A2/es
Publication of WO2011009975A3 publication Critical patent/WO2011009975A3/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Método para la detección e identificación de especies de la familia engraulidae. La presente invención se refiere a un método para la detección e identificación simultánea en una muestra de al menos una de las especies de la familia Engraulidae seleccionada entre Engraulis encrasicolus, Engraulis mordax y Engraulis ringens, mediante amplificación por PCR multiplex de al menos una de la secuencias, seleccionada de entre SEQ ID NO 1, SEQ ID NO 2 y SEQ ID NO 3, correspondiente con el espaciador no transcrito (NTS) del ADN ribosómico 5S de al menos una de las 3 especies, utilizando al menos uno de los pares de cebadores de secuencia seleccionados entre: SEQ ID NO 4 y SEQ ID NO 5; SEQ ID NO 6 y SEQ ID NO 7; y SEQ ID NO 8 y SEQ ID NO 9.
PCT/ES2010/000314 2009-07-22 2010-07-19 Método para la detección e identificación de especies de la familia engraulidae WO2011009975A2 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ESP200901649 2009-07-22
ES200901649A ES2376683B1 (es) 2009-07-22 2009-07-22 Método para la detección e identificación de especies de la familia engraulidae.

Publications (2)

Publication Number Publication Date
WO2011009975A2 WO2011009975A2 (es) 2011-01-27
WO2011009975A3 true WO2011009975A3 (es) 2011-07-14

Family

ID=43499465

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/ES2010/000314 WO2011009975A2 (es) 2009-07-22 2010-07-19 Método para la detección e identificación de especies de la familia engraulidae

Country Status (2)

Country Link
ES (1) ES2376683B1 (es)
WO (1) WO2011009975A2 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2392610B1 (es) * 2011-04-04 2013-11-13 Universidad Del Pais Vasco - Euskal Herriko Unibertsitatea Método de identificación genética de la anchoa europea y de su origen geográfico o población.
NO337188B1 (no) * 2013-04-08 2016-02-08 Niva COMPAS-PCR fremgangsmåte og fremgangsmåter for påvisning, identifisering eller overvåking av laksearter, kit, samt anvendelse av metoden og kit
CN109439739A (zh) * 2018-08-16 2019-03-08 浙江海洋大学 黄鲫高密度snp标记筛选方法和应用

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
ASENSIO, L.: "Application of multiplex PCR for the identification of grouper meals in the restaurant industry.", FOOD CONTROL., vol. 19, no. 11, November 2008 (2008-11-01), pages 1096 - 1999, XP022708604, doi:10.1016/j.foodcont.2007.11.002 *
HICHAM, C. ET AL.: "Analisis molecular de familias multigenicas ribosomales y de snRNAs y su uso como herramientas para diferenciar entre especies de la famila de Engraulidae. XVII Seminario de Genetica de Poblaciones y Evolucion.", RIBADESELLA., November 2008 (2008-11-01), pages 30., Retrieved from the Internet <URL:http://www.segenetica.es/secciones/resumenes/XVIISGPERibadesella2008.pdf> [retrieved on 20101124] *
MARTINS, C. ET AL.: "Organization and evolution of 5S ribosomal DNA in the fish genome.", FOCUS ON GENOME RESEARCH., 2004, pages 335 - 363 *
PINHAL, D. ET AL.: "Discrimination of shark species by simple PCR of 5S rDNA repeats.", GENETICS AND MOLECULAR BIOLOGY., vol. 31, no. 1, April 2008 (2008-04-01), pages 361 - 365 *
RASMUSSEN, R. S. ET AL.: "Application of DNA-based methods to identify fish and seafood substitution on the commercial market.", COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY., vol. 8, no. 2, April 2009 (2009-04-01), pages 118 - 154, XP055094193, doi:10.1111/j.1541-4337.2009.00073.x *
RASMUSSEN, R. S. ET AL.: "DNA-based methods for the identification of commercial fish and seafood species.", COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY., vol. 7, no. 3, June 2008 (2008-06-01), pages 280 - 295, XP055094196, doi:10.1111/j.1541-4337.2008.00046.x *
WASKO, A. P. ET AL.: "Molecular organization of 5S rDNA in fishes of the genus Brycon.", GENOME., vol. 44, no. 5, October 2001 (2001-10-01), pages 893 - 902 *

Also Published As

Publication number Publication date
ES2376683A1 (es) 2012-03-16
WO2011009975A2 (es) 2011-01-27
ES2376683B1 (es) 2013-01-28

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