[go: up one dir, main page]

WO2010147395A3 - Medium composition comprising neuropeptide y for the generation, maintenance, prologned undifferentiated growth of pluripotent stem cells and method of culturing pluripotent stem cell using the same - Google Patents

Medium composition comprising neuropeptide y for the generation, maintenance, prologned undifferentiated growth of pluripotent stem cells and method of culturing pluripotent stem cell using the same Download PDF

Info

Publication number
WO2010147395A3
WO2010147395A3 PCT/KR2010/003891 KR2010003891W WO2010147395A3 WO 2010147395 A3 WO2010147395 A3 WO 2010147395A3 KR 2010003891 W KR2010003891 W KR 2010003891W WO 2010147395 A3 WO2010147395 A3 WO 2010147395A3
Authority
WO
WIPO (PCT)
Prior art keywords
pluripotent stem
stem cells
neuropeptide
same
medium composition
Prior art date
Application number
PCT/KR2010/003891
Other languages
French (fr)
Other versions
WO2010147395A2 (en
Inventor
Yee Sook Cho
Mi-Young Son
Original Assignee
Korea Research Institute Of Bioscience And Biotechnology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020100056696A external-priority patent/KR101166257B1/en
Application filed by Korea Research Institute Of Bioscience And Biotechnology filed Critical Korea Research Institute Of Bioscience And Biotechnology
Priority to US13/378,912 priority Critical patent/US8628963B2/en
Publication of WO2010147395A2 publication Critical patent/WO2010147395A2/en
Publication of WO2010147395A3 publication Critical patent/WO2010147395A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/80—Neurotransmitters; Neurohormones
    • C12N2501/835—Neuropeptide Y [NPY]; Peptide YY [PYY]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The present invention relates to a medium composition comprising neuropeptide Y, effective for proliferation and maintenance of undifferentiated pluripotent stem cells, and a method for culturing undifferentiated pluripotent stem cells using the same. The present invention improves the culture conditions for undifferentiated pluripotent stem cells, and ultimately, the present invention can be effectively used for the development of large-scale culture systems, thereby acquiring clinically applicable pluripotent stem cells. Further, the present invention relates to a dedifferentiation medium composition comprising neuropeptide Y (NPY), and a method for inducing dedifferentiation(or reprogramming) using the same. The present invention improves the culture conditions for dedifferentiation and contributes to develop technology of producing clinically applicable induced pluripotent stem cells, thereby being used for the development of stem cell therapy.
PCT/KR2010/003891 2009-06-16 2010-06-16 Medium composition comprising neuropeptide y for the generation, maintenance, prologned undifferentiated growth of pluripotent stem cells and method of culturing pluripotent stem cell using the same WO2010147395A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US13/378,912 US8628963B2 (en) 2009-06-16 2010-06-16 Medium composition comprising neuropeptide Y for the generation, maintenance, prolonged undifferentiated growth of pluripotent stem cells and method of culturing pluripotent stem cell using the same

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR10-2009-0053383 2009-06-16
KR20090053383 2009-06-16
KR1020100056696A KR101166257B1 (en) 2009-06-16 2010-06-15 Media composition comprising neuropeptide y for the generation, maintenance, prologned undifferentiated growth of pluripotent stem cells and method of culturing pluripotent stem cell using the same
KR10-2010-0056696 2010-06-15

Publications (2)

Publication Number Publication Date
WO2010147395A2 WO2010147395A2 (en) 2010-12-23
WO2010147395A3 true WO2010147395A3 (en) 2011-08-25

Family

ID=43356920

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2010/003891 WO2010147395A2 (en) 2009-06-16 2010-06-16 Medium composition comprising neuropeptide y for the generation, maintenance, prologned undifferentiated growth of pluripotent stem cells and method of culturing pluripotent stem cell using the same

Country Status (1)

Country Link
WO (1) WO2010147395A2 (en)

Families Citing this family (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2843234C (en) 2011-07-25 2019-08-13 Kyoto University Method for screening induced pluripotent stem cells
JP6162604B2 (en) 2011-10-21 2017-07-12 国立大学法人京都大学 Multi-dispersed monodisperse cell culture method by laminar flow
JP5999658B2 (en) 2011-11-25 2016-09-28 国立大学法人京都大学 Method for culturing pluripotent stem cells
EP2955223B1 (en) 2013-02-08 2019-12-18 Kyoto University Production methods for megakaryocytes and platelets
WO2014136581A1 (en) 2013-03-06 2014-09-12 国立大学法人京都大学 Culture system for pluripotent stem cells and method for subculturing pluripotent stem cells
US10519421B2 (en) 2013-03-21 2019-12-31 Kyoto University Induction of motor neurons from pluripotent stem cells
US10072242B2 (en) 2013-03-25 2018-09-11 Foundation For Biomedical Research And Innovation At Kobe Cell sorting method
JP6461787B2 (en) 2013-04-12 2019-01-30 国立大学法人京都大学 Method for inducing alveolar epithelial progenitor cells
US9822342B2 (en) 2013-05-14 2017-11-21 Kyoto University Method of efficiently inducing cardiomyocytes
US10159766B2 (en) 2013-05-31 2018-12-25 Iheart Japan Corporation Layered cell sheet incorporating hydrogel
RU2696315C2 (en) 2013-06-11 2019-08-01 Киото Юниверсити Method of producing renal progenitor cells and medicinal agent containing same
WO2015020113A1 (en) 2013-08-07 2015-02-12 国立大学法人京都大学 Method for producing pancreatic hormone-producing cell
CN105849255A (en) 2013-09-05 2016-08-10 国立大学法人京都大学 New method for inducing dopamine-producing neural precursor cells
EP3064577B1 (en) 2013-11-01 2020-09-09 Kyoto University Novel chondrocyte induction method
EP3929302A1 (en) 2014-07-14 2021-12-29 Chugai Seiyaku Kabushiki Kaisha Method for identifying epitope on protein
CN114292817A (en) 2014-07-18 2022-04-08 国立大学法人京都大学 Method for inducing T cells for cellular immunotherapy from pluripotent stem cells
WO2016104717A1 (en) 2014-12-26 2016-06-30 国立大学法人京都大学 Hepatocyte induction method
EP3081638A1 (en) 2015-04-16 2016-10-19 Kyoto University Method for producing pseudo-islets
WO2017179720A1 (en) 2016-04-15 2017-10-19 国立大学法人京都大学 Method for inducing cd8+ t cells
SG11201809279YA (en) 2016-04-22 2018-11-29 Univ Kyoto Method for producing dopamine-producing neural precursor cells
TWI814716B (en) 2016-12-27 2023-09-11 日商住友化學股份有限公司 Evaluating method and selecting method of induced pluripotent stem cells, and manufacturing method of induced pluripotent stem cells
WO2018135646A1 (en) 2017-01-20 2018-07-26 国立大学法人京都大学 METHOD FOR PRODUCING CD8α+β+ CYTOTOXIC T CELLS
US20210130785A1 (en) 2017-01-26 2021-05-06 Osaka University Medium for inducing differentiation of stem cells into mesodermal cells and method for producing mesodermal cells
EP3597734A4 (en) 2017-03-14 2021-03-03 Kyoto University Method for producing helper t cells from pluripotent stem cells
CN117802033A (en) 2017-05-25 2024-04-02 国立大学法人京都大学 Method for producing mesoderm lineage primitive streak cells from pluripotent stem cells
WO2018235583A1 (en) 2017-06-19 2018-12-27 公益財団法人神戸医療産業都市推進機構 Method for predicting differentiation ability of pluripotent stem cell, and reagent for same
WO2019078263A1 (en) 2017-10-17 2019-04-25 国立大学法人京都大学 Method for obtaining artificial neuromuscular junction from pluripotent stem cells
US20210130777A1 (en) 2018-07-13 2021-05-06 Kyoto University Method for producing gamma delta t cells
JP7285015B2 (en) 2018-07-19 2023-06-01 国立大学法人京都大学 Lamellar cartilage derived from pluripotent stem cells and method for producing the same
US20210332329A1 (en) 2018-07-23 2021-10-28 Kyoto University Novel renal progenitor cell marker and method for concentrating renal progenitor cells using same
US12168781B2 (en) 2018-12-06 2024-12-17 Kirin Holdings Kabushiki Kaisha Production method for T cells or NK cells, medium for culturing T cells or NK cells, method for culturing T cells or NK cells, method for maintaining undifferentiated state of undifferentiated T cells, and growth-accelerating agent for T cells or NK cells
US20220056413A1 (en) 2018-12-21 2022-02-24 Kyoto University Lubricin-localized cartilage-like tissue, method for producing same and composition comprising same for treating articular cartilage damage
WO2020138371A1 (en) 2018-12-26 2020-07-02 キリンホールディングス株式会社 Modified tcr and production method therefor
EP3957722A4 (en) 2019-04-17 2023-01-18 Keio University Production method and kit of induced pluripotent stem cells
CA3140384A1 (en) 2019-05-15 2020-11-19 Ajinomoto Co., Inc. Method for purifying neural crest cells or corneal epithelial cells
CA3141455A1 (en) 2019-05-20 2020-11-26 Ajinomoto Co., Inc. Expansion culture method for cartilage or bone precursor cells
JP7058431B2 (en) 2019-12-12 2022-04-22 国立大学法人千葉大学 Freeze-dried preparation containing megakaryocytes and platelets
US20210292713A1 (en) 2020-02-28 2021-09-23 Millennium Pharmaceuticals, Inc. Method for producing natural killer cells from pluripotent stem cells
JPWO2021256522A1 (en) 2020-06-17 2021-12-23
EP4180516A4 (en) 2020-07-13 2024-01-17 Kyoto University Skeletal muscle precursor cells and method for purifying same, composition for treating myogenic diseases, and method for producing cell group containing skeletal muscle precursor cells
CN116134130A (en) 2020-07-20 2023-05-16 学校法人爱知医科大学 Composition for maintaining and culturing undifferentiated pluripotent cells, medium for maintaining and culturing undifferentiated pluripotent cells, method for maintaining and culturing pluripotent cells in an undifferentiated state, and method for producing pluripotent cells
EP4202041A4 (en) 2020-08-18 2024-10-16 Kyoto University METHODS FOR MAINTAINING AND AMPLIFICATION OF HUMAN PRIMORDIAL GERM CELLS / HUMAN PRIMORDIAL GERM CELL-LIKE CELLS
JPWO2022196714A1 (en) 2021-03-17 2022-09-22
WO2022230977A1 (en) 2021-04-30 2022-11-03 国立研究開発法人理化学研究所 Cord-like aggregates of retinal pigment epithelial cells, device and production method for producing same, and therapeutic agent comprising said cord-like aggregates
EP4349968A1 (en) 2021-06-04 2024-04-10 Kirin Holdings Kabushiki Kaisha Cell composition, method for producing cell composition, and pharmaceutical composition containing cell composition
CA3222761A1 (en) 2021-06-10 2022-12-15 Ajinomoto Co., Inc. Method for producing mesenchymal stem cells
AU2022292988A1 (en) 2021-06-15 2024-01-04 Takeda Pharmaceutical Company Limited Method for producing natural killer cells from pluripotent stem cells
JPWO2023286834A1 (en) 2021-07-15 2023-01-19
WO2023286832A1 (en) 2021-07-15 2023-01-19 アステラス製薬株式会社 Pericyte-like cells expressing vascular endothelial growth factor (vegf) at high level
WO2023017848A1 (en) 2021-08-11 2023-02-16 国立大学法人京都大学 Method for producing renal interstitial progenitor cells, erythropoietin-producing cells, and method for producing renin-producing cells
WO2023085356A1 (en) 2021-11-11 2023-05-19 株式会社ヘリオス Gene-modified pluripotent stem cell, immunocompetent cell derived therefrom, method for producing said cells, and use thereof
WO2023153464A1 (en) 2022-02-09 2023-08-17 住友ファーマ株式会社 Method for assessing differentiation potential of cells in culture broth in differentiation of pluripotent stem cells into neural cells of midbrain floor plate region
WO2023210713A1 (en) 2022-04-27 2023-11-02 国立大学法人京都大学 Epicardial cell regeneration promoter and method for promoting epicardial cell regeneration
WO2023238932A1 (en) 2022-06-10 2023-12-14 国立大学法人京都大学 Detection method and detection reagent for undifferentiated pluripotent stem cells

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030095956A1 (en) * 1991-07-08 2003-05-22 Samuel Weiss Methods of proliferating undifferentiated neural cells
US20040082003A1 (en) * 2000-02-23 2004-04-29 Guy Sauvageau Stem cell expansion enhancing factor and method of use
WO2008043181A1 (en) * 2006-10-12 2008-04-17 Mcgill University Augmenting stem cell populations by modulating t-cell protein tyrosine phosphatase (tc-ptp)
WO2008129563A2 (en) * 2007-04-23 2008-10-30 Stempeutics Research Private Limited, Human mesenchymal stem cells and preparation thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030095956A1 (en) * 1991-07-08 2003-05-22 Samuel Weiss Methods of proliferating undifferentiated neural cells
US20040082003A1 (en) * 2000-02-23 2004-04-29 Guy Sauvageau Stem cell expansion enhancing factor and method of use
WO2008043181A1 (en) * 2006-10-12 2008-04-17 Mcgill University Augmenting stem cell populations by modulating t-cell protein tyrosine phosphatase (tc-ptp)
WO2008129563A2 (en) * 2007-04-23 2008-10-30 Stempeutics Research Private Limited, Human mesenchymal stem cells and preparation thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
OWAIN W. HOWELL, ET AL.: "Neuropeptide Y stimulates neuronal precursor proliferation in the post-natal and adult dentate gyrus", J. NEUROCHEM., vol. 93, no. 3, May 2005 (2005-05-01), pages 560 - 570 *

Also Published As

Publication number Publication date
WO2010147395A2 (en) 2010-12-23

Similar Documents

Publication Publication Date Title
WO2010147395A3 (en) Medium composition comprising neuropeptide y for the generation, maintenance, prologned undifferentiated growth of pluripotent stem cells and method of culturing pluripotent stem cell using the same
EP4368701A3 (en) Feeder-free culture system
EP2408903A4 (en) CULTURE OF PLURIPOTENT AND MULTIPOTENT STEM CELLS ON MICROSUPPORTS
IL252607B (en) Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
MX356756B (en) Pluripotent stem cell culture on micro-carriers.
SE0850111L (en) Procedure for differentiating stem cells into cells of the endodermal and pancreatic developmental line
WO2008129563A3 (en) Human mesenchymal stem cells and preparation thereof
EP2307539A4 (en) METHOD FOR EFFICIENT MANUFACTURE OF INDUCED PLURIPOTENTER STEM CELLS
GB2454386A (en) Method for stem cell culture and cells derived therefrom
WO2009077134A3 (en) Use of rna for reprogramming somatic cells
WO2010129294A3 (en) Small molecules supporting pluripotent cell growth and methods thereof
WO2008036447A3 (en) Pluripotent stem cell culture
MX2011013385A (en) Methods for culturing stem and progenitor cells.
EP2438161A4 (en) METHOD FOR THE PRODUCTION OF INDUCED PLURIPOTENTER STEM CELLS AND METHOD FOR THEIR CULTIVATION
WO2006070370A3 (en) Stem cells culture systems
WO2012087965A3 (en) Cell culture platform for single cell sorting and enhanced reprogramming of ipscs
WO2010124142A3 (en) Cell compositions derived from dedifferentiated reprogrammed cells
MX2008001709A (en) Production of reprogrammed cells with restored potential.
SG195299A1 (en) Expansion of stem cells in hollow fiber bioreactors
WO2012052854A3 (en) Plant cell lines and methods of isolating the same
PH12014501175A1 (en) Medium composition for rejuvenating stem cells
WO2009028631A1 (en) Agent for promoting corneal endothelial cell adhesion
WO2007115337A3 (en) Chondrocyte differentiation from human embryonic stem cells and their use in tissue engineering
SG179458A1 (en) Growth medium for c. histolyticum without ingredients of mamalian sources
WO2014027176A3 (en) Stem cell culture method

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 10789722

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 13378912

Country of ref document: US

122 Ep: pct application non-entry in european phase

Ref document number: 10789722

Country of ref document: EP

Kind code of ref document: A2