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WO2010051990A1 - Support de sonde et procédé d'obtention de résultats d'analyse comparables par orientation des substances sur un plan uniforme - Google Patents

Support de sonde et procédé d'obtention de résultats d'analyse comparables par orientation des substances sur un plan uniforme Download PDF

Info

Publication number
WO2010051990A1
WO2010051990A1 PCT/EP2009/007914 EP2009007914W WO2010051990A1 WO 2010051990 A1 WO2010051990 A1 WO 2010051990A1 EP 2009007914 W EP2009007914 W EP 2009007914W WO 2010051990 A1 WO2010051990 A1 WO 2010051990A1
Authority
WO
WIPO (PCT)
Prior art keywords
sample carrier
substances
recesses
substance
vessel
Prior art date
Application number
PCT/EP2009/007914
Other languages
German (de)
English (en)
Inventor
Andreas Fischer
Dagmar Lischke
Dagmar Peters-Groth
Original Assignee
Grünenthal GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Grünenthal GmbH filed Critical Grünenthal GmbH
Priority to EP09749018.9A priority Critical patent/EP2355929B1/fr
Priority to ES09749018.9T priority patent/ES2619831T3/es
Publication of WO2010051990A1 publication Critical patent/WO2010051990A1/fr
Priority to US13/099,916 priority patent/US20110245106A1/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/06—Test-tube stands; Test-tube holders
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0642—Filling fluids into wells by specific techniques
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50855—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates using modular assemblies of strips or of individual wells

Definitions

  • the present invention relates to a sample carrier, for example a multiwell platform, with one or a plurality of recesses, in each of which a substance to be analyzed is located.
  • the present invention further relates to a method in which one or more substances, which are each located in a recess of a sample carrier, are aligned prior to their analysis on a uniform plane relative to the surface of the sample carrier.
  • the object is achieved with a sample carrier having a plurality of recesses, in each of which a substance to be analyzed is located, wherein it has a means with which the substances to be analyzed can be aligned respectively to the same plane relative to the sample carrier.
  • the sample carrier according to the invention is, for example, a so-called multiwell platform.
  • the sample carrier has one or any multiplicity of recesses, in particular bores, which are preferably arranged in a uniform pattern, very particularly preferably equidistantly are.
  • the recesses extend through the entire thickness of the sample carrier.
  • the recesses may have any cross-section, but are preferably circular.
  • the recesses are lined with a sleeve, in particular a sleeve, on which the least possible adhesion of the substances takes place. For example, it is a Teflon sleeve.
  • each recess for example, coated with a non-stick coating, and / or the entire sample carrier is made of an inert material, which particularly preferably also has non-stick properties.
  • a vessel is preferably arranged in each case displaceably along the recess.
  • the vessel may be part of a stamp, in particular be arranged on its upper side.
  • the stamp can be made of glass, metal, especially steel and / or plastic. He may also have a non-stick coating.
  • This vessel can be configured as desired. For example, it may be flat but also convex and / or concave.
  • the substance to be analyzed is located in the vessel and / or on the vessel.
  • the height of the substance preferably extends at least partially at least to the upper edge of the vessel, but is particularly preferably beyond.
  • the substance to be analyzed can each be placed on the vessel.
  • the production of the respective substance takes place in the vessel, so that the vessel can also be designed as a production vessel.
  • the preparation preferably takes place in a comparatively lowered state of the vessel within the sample carrier.
  • the vessel may be made of glass, metal, in particular steel, and / or plastic. It may also have a non-stick coating.
  • the sample carrier now has a means with which the substance to be analyzed can be aligned relative to the surface of the sample carrier, in each case on the same plane, in particular horizontal plane, preferably above the sample carrier.
  • This means is preferably a covering means covering one side of each recess.
  • the respective substance is moved along the recess in the direction of the cover before its analysis, in particular displaced, which, for example, with the above-described Stamp can be made, which is pressed from below against the vessel.
  • the substance to be analyzed in each case can also simply be filled into the respective recess, then it falls onto the cover and preferably remains at least partially attached thereto.
  • each substance and / or the respective vessel comes into contact with the covering means, so that these are then all in the same plane relative to the surface of the sample carrier. Accordingly, in the case of the sample carrier according to the invention, all substances have the same position, at least in some areas, relative to the surface of the sample carrier. As a result, the quality of the comparability of the analysis, for example, in the Röntengenpulverdiffraktometriefug in reflection is increased.
  • the covering means of the respective substance and / or the respective vessel serves as a stop. Preferably, these are pressed against the cover and particularly preferably pressed flat.
  • the covering means is preferably provided in one piece and therefore covers all ends of the existing recesses on one side of the sample carrier. Particularly preferably, the transmission of the covering agent is as high as possible, in particular with regard to X-rays.
  • the covering means is preferably a foil, in particular an X-ray-permeable foil.
  • a film according to the invention has a thickness in the micron range.
  • the covering means is a plate, in particular an X-ray transparent plate, which has a higher mechanical stability than a foil and in particular is not damaged or plastically deformed by the substances and / or the vessels.
  • the covering means can also be used to prevent cross-contamination of the substances with one another.
  • a spacer is arranged between the sample carrier and the cover.
  • the cover means in particular reversibly, is then preferably arranged on this spacer.
  • the covering means in particular if it is a film, adhered to the spacer.
  • the spacer has recesses, for example in the form of through holes, which are coaxial with the recesses of the Sample carrier are arranged.
  • the thickness of this spacer is greater than the amount by which the sleeves, which are arranged in the recesses of the sample holder, protrude from the surface of the sample carrier. As a rule, a thickness of a few millimeters is sufficient.
  • the area of the recess in the spacer is equal to or greater than the area of the underlying recess in the sample carrier.
  • the area of each recess in the spacer is so large that it can receive the outer edge of the sleeves which are located in the recesses in the sample carrier.
  • the spacer is not located on the edge of a sleeve but directly on the respective surface of the sample carrier. Now, if the substances are moved exactly to the outer edge of the spacer, where the covering is as a stop, they are all aligned exactly in the same plane relative to the surface of the sample carrier. For example, with a horizontally oriented sample carrier, they are located at exactly the same height above it.
  • a locking means is arranged on the side facing away from the sample carrier of the cover.
  • This counter-agent increases in particular the mechanical strength of the cover and is particularly advantageous if the cover is a film.
  • the securing means is preferably fastened to the sample carrier such that the covering means is located between the securing means and the sample carrier.
  • the locking means it is ensured that the substances and / or the vessels do not damage the covering means when they are brought into contact with it.
  • the counter-agent the substances can be pressed against the covering means with comparatively high force, without the covering means changing its position and / or being mechanically destroyed.
  • the counter-agent can also serve as a stop for the substances and / or the vessels. For the actual analysis, the counter-agent is then preferably removed from the sample carrier.
  • the sample carrier according to the invention is suitable for any analysis, but in particular for high-throughput screening and in particular for the analysis of reflection measurements by means of X-ray powder diffractometry.
  • Another object of the present invention is a method for analyzing a plurality of substances, each located in the recess of a sample carrier, in which the substances are aligned prior to their analysis on a uniform plane relative to the sample carrier.
  • Alignment may be by manual or mechanical force and / or gravity.
  • sample carrier according to the invention applies equally to the method according to the invention and vice versa.
  • the method according to the invention can be carried out in particular with the sample carrier according to the invention.
  • the recesses are covered with a covering.
  • This covering means serves all samples and / or the vessel, on or in which they are located, preferably as a stop, but also to avoid cross-contamination of the substances with each other.
  • the substances are brought to the cover means to the plant, preferably pressed against it.
  • the substances to be analyzed are in each case in and / or on a vessel which is arranged longitudinally displaceable in each case in a recess of the sample carrier, these vessels are moved in the direction of the cover before the analysis of the substances until the substance and / or the vessel touches the cover in each case.
  • FIGS 1- 1-5 These explanations are merely exemplary and do not limit the general inventive concept. The explanations apply equally to both subjects of the invention.
  • FIG. 1 shows a sample carrier, here a multiwell platform.
  • FIG. 2 shows a stamp with a vessel.
  • FIG. 3 shows the spacer
  • FIG. 4 shows the counter plate.
  • FIG. 5 shows the sample carrier before the measurement.
  • FIG. 1 shows the sample carrier 1 according to the invention in two views.
  • This is a multiwell plate which has a multiplicity, here 96, of through holes 2, which therefore extend over the entire thickness D of the sample carrier.
  • the holes 2 are arranged equidistantly in a uniform pattern.
  • Each hole is lined with a Teflon sleeve 8, which protrudes by a certain amount over the surface 1.1 of the sample carrier, this amount varies, usually max. Does not exceed 2 mm.
  • the Teflon sleeve has an outer diameter 8.1.
  • a punch 9 is arranged, which is shown in Figure 2.
  • the stamp is made of steel and / or Teflon and has at its upper end a preferably flat glass plate 3, the vessel on which the substance to be analyzed 4 arranged and / or on which the substance to be analyzed is prepared.
  • the diameter of the punch 9 and the vessel 3 is dimensioned so that the punch can be moved with moderate effort within the sleeve.
  • Figure 3 shows the spacer 5, which also has a plurality of through holes 5.1, whose arrangement corresponds to the arrangement of the holes 2 in the sample carrier, so that the center of the holes 2, 5.1 is aligned when the spacer 5 is placed on the sample carrier 1.
  • the diameter 5.2 of the holes 5.1 is chosen so that it is at least equal, preferably slightly larger than the outer diameter of the sleeves 8, so that the upper edge of the recesses 5.1 can be added.
  • the spacer 5 can be connected by four screws in the corner with the sample carrier. After the spacer 5 has been placed on the sample carrier 1, the edge of any sleeve is beyond the surface of the spacer.
  • a glue a water-soluble adhesive has proven itself.
  • the surface of the spacer 5 is at least partially coated with the adhesive and then the film 6 glued.
  • the counter plate 7 which is shown in Figure 4, attached to the glued-with-foil spacer, for example screwed.
  • the analysis of the substances is carried out as follows. In each case a substance 4 is placed on the vessel 3 of a sample carrier or produced therein. The vessels 3 and the punches 9 are in a comparatively lowered position. Thereafter, or before the adhered with a film 6 spacer 5 is mounted on the surface 1.1 of the sample carrier 1. Then the counter plate is attached. Thereafter, the sample carrier is rotated together with spacer 5, slide 6 and counter plate 7 by 180 °, so that the counter plate points down. Subsequently, the punches 9 and thus the vessels 3 are manually or mechanically displaced in the direction of the covering means 6, until the edge 3.1 of the vessels 3 rests against the covering means 6, respectively.
  • the counter plate 7 prevents the film 6 is damaged by the vessels 3 in particular overstretched. But the counter plate also serves as a stop for the vessels / stamp, so that the edge 3.1 of all vessels and the position of the substances 4 after moving the stamp has exactly the same position relative to the surface of the sample carrier 1, i. the substances 4 and / or the edge 3.1 then all have the same distance from the surface 1.1 of the sample carrier.
  • the substances 4 can partially stick to the film 6. Thereafter, the sample carrier is rotated back by 180 ° and now the counter plate 7 removed from the sample carrier 1 and the sample carrier preferably horizontally mounted on an analyzer and carried out the substance by substance analysis.
  • the substances all have the same height, which is advantageous for the comparability of the measurements.
  • the covering means itself has sufficient mechanical stability that it is not damaged or plastically deformed by the substances and / or the vessels.
  • the sample carrier provided with the covering means only, as described above, aligned with the covering means down and each substance to be analyzed are each filled into a bore, which then falls on the cover and clinging there. Then the sample carrier can be turned back and the analysis carried out.
  • FIG. 5 shows the sample carrier after the alignment. It can be clearly seen how the substances on the film 6, which is glued to the spacer 5 adheres. All substances are at the same height.
  • reaction vessel 3 vessel, reaction vessel, glass plate 3.1 upper edge of the reaction vessel

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

La présente invention concerne un support de sonde, par exemple une plateforme multipuits avec une ou une pluralité de cavités dans chacune desquelles se trouve une substance à analyser. La présente invention concerne en outre un procédé suivant lequel une ou plusieurs substances, qui se trouvent chacune dans une cavité d'un support de sonde, sont orientées avant leur analyse sur un plan uniforme par rapport à la surface du support de sonde.
PCT/EP2009/007914 2008-11-06 2009-11-05 Support de sonde et procédé d'obtention de résultats d'analyse comparables par orientation des substances sur un plan uniforme WO2010051990A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP09749018.9A EP2355929B1 (fr) 2008-11-06 2009-11-05 Porte-échantillons et procédé d'obtention de résultats d'analyse comparables par alignement des substances sur une surface homogène
ES09749018.9T ES2619831T3 (es) 2008-11-06 2009-11-05 Porta-muestras y procedimiento de obtención de resultados de análisis comparables mediante la alineación de sustancias en un plano común
US13/099,916 US20110245106A1 (en) 2008-11-06 2011-05-03 Sample Carrier and Method for Achieving Comparable Analytical Results By Aligning Test Substances on a Uniform Plane

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP08019415 2008-11-06
EP08019415.2 2008-11-06

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US13/099,916 Continuation US20110245106A1 (en) 2008-11-06 2011-05-03 Sample Carrier and Method for Achieving Comparable Analytical Results By Aligning Test Substances on a Uniform Plane

Publications (1)

Publication Number Publication Date
WO2010051990A1 true WO2010051990A1 (fr) 2010-05-14

Family

ID=40521436

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2009/007914 WO2010051990A1 (fr) 2008-11-06 2009-11-05 Support de sonde et procédé d'obtention de résultats d'analyse comparables par orientation des substances sur un plan uniforme

Country Status (4)

Country Link
US (1) US20110245106A1 (fr)
EP (1) EP2355929B1 (fr)
ES (1) ES2619831T3 (fr)
WO (1) WO2010051990A1 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4128698A1 (de) * 1991-08-29 1993-03-04 Boehringer Mannheim Gmbh Analysesystem
DE19742160A1 (de) * 1996-09-24 1998-04-02 Hitachi Ltd Analysiervorrichtung mit Funktion zum Pipettieren von Proben
US20030085952A1 (en) * 2001-11-05 2003-05-08 Williams Roger O Apparatus and method for controlling the free surface of liquid in a well plate
EP1614476A1 (fr) * 2004-07-06 2006-01-11 Boehringer Mannheim Gmbh Système de stockage
EP1790414A1 (fr) * 2005-11-23 2007-05-30 Tecan Trading AG Tubes d'échantillons et système pour le conditionnement et la fourniture d'échantillons d'acides nucléiques

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3987719A (en) * 1973-07-23 1976-10-26 Leonard Kian Cooking utensil
US6258325B1 (en) * 1993-04-19 2001-07-10 Ashok Ramesh Sanadi Method and apparatus for preventing cross-contamination of multi-well test plates
US5342581A (en) * 1993-04-19 1994-08-30 Sanadi Ashok R Apparatus for preventing cross-contamination of multi-well test plates
US6241949B1 (en) * 1999-08-17 2001-06-05 Spectrumedix Corporation Spill-resistant microtitre trays and method of making
US6677162B1 (en) * 2000-07-18 2004-01-13 Uop Llc Process of parallel sample preparation

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4128698A1 (de) * 1991-08-29 1993-03-04 Boehringer Mannheim Gmbh Analysesystem
DE19742160A1 (de) * 1996-09-24 1998-04-02 Hitachi Ltd Analysiervorrichtung mit Funktion zum Pipettieren von Proben
US20030085952A1 (en) * 2001-11-05 2003-05-08 Williams Roger O Apparatus and method for controlling the free surface of liquid in a well plate
EP1614476A1 (fr) * 2004-07-06 2006-01-11 Boehringer Mannheim Gmbh Système de stockage
EP1790414A1 (fr) * 2005-11-23 2007-05-30 Tecan Trading AG Tubes d'échantillons et système pour le conditionnement et la fourniture d'échantillons d'acides nucléiques

Also Published As

Publication number Publication date
US20110245106A1 (en) 2011-10-06
EP2355929A1 (fr) 2011-08-17
ES2619831T3 (es) 2017-06-27
EP2355929B1 (fr) 2016-12-21

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