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WO2010011944A3 - Protein screeing methods - Google Patents

Protein screeing methods Download PDF

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Publication number
WO2010011944A3
WO2010011944A3 PCT/US2009/051716 US2009051716W WO2010011944A3 WO 2010011944 A3 WO2010011944 A3 WO 2010011944A3 US 2009051716 W US2009051716 W US 2009051716W WO 2010011944 A3 WO2010011944 A3 WO 2010011944A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
methods
screeing
screeing methods
vitro
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2009/051716
Other languages
French (fr)
Other versions
WO2010011944A2 (en
Inventor
Richard W. Wagner
Alexander Litovchick
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to CN200980137939XA priority Critical patent/CN102177438A/en
Priority to JP2011520232A priority patent/JP5924937B2/en
Priority to ES09801086T priority patent/ES2752025T3/en
Priority to EP09801086.1A priority patent/EP2316030B1/en
Priority to EP19192666.6A priority patent/EP3629022A1/en
Application filed by Individual filed Critical Individual
Publication of WO2010011944A2 publication Critical patent/WO2010011944A2/en
Publication of WO2010011944A3 publication Critical patent/WO2010011944A3/en
Priority to US13/009,500 priority patent/US9134304B2/en
Anticipated expiration legal-status Critical
Priority to US14/796,480 priority patent/US9803193B2/en
Priority to US15/703,710 priority patent/US10519438B2/en
Priority to US16/678,220 priority patent/US11268090B2/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1062—Isolating an individual clone by screening libraries mRNA-Display, e.g. polypeptide and encoding template are connected covalently
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention provides methods and compositions useful for identifying polypeptides with desired characteristics in vitro.
PCT/US2009/051716 2008-07-25 2009-07-24 Protein screeing methods Ceased WO2010011944A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
CN200980137939XA CN102177438A (en) 2008-07-25 2009-07-24 protein screening method
JP2011520232A JP5924937B2 (en) 2008-07-25 2009-07-24 Protein screening method
ES09801086T ES2752025T3 (en) 2008-07-25 2009-07-24 Protein screening methods
EP09801086.1A EP2316030B1 (en) 2008-07-25 2009-07-24 Protein screeing methods
EP19192666.6A EP3629022A1 (en) 2008-07-25 2009-07-24 Protein screening methods
US13/009,500 US9134304B2 (en) 2008-07-25 2011-01-19 Protein screening methods
US14/796,480 US9803193B2 (en) 2008-07-25 2015-07-10 Protein screening methods
US15/703,710 US10519438B2 (en) 2008-07-25 2017-09-13 Protein screening methods
US16/678,220 US11268090B2 (en) 2008-07-25 2019-11-08 X-display complexes for protein screening methods

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US8381308P 2008-07-25 2008-07-25
US61/083,813 2008-07-25
US9011108P 2008-08-19 2008-08-19
US61/090,111 2008-08-19
US17002909P 2009-04-16 2009-04-16
US61/170,029 2009-04-16

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US13/009,500 Continuation US9134304B2 (en) 2008-07-25 2011-01-19 Protein screening methods

Publications (2)

Publication Number Publication Date
WO2010011944A2 WO2010011944A2 (en) 2010-01-28
WO2010011944A3 true WO2010011944A3 (en) 2010-05-06

Family

ID=41570886

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/051716 Ceased WO2010011944A2 (en) 2008-07-25 2009-07-24 Protein screeing methods

Country Status (7)

Country Link
US (4) US9134304B2 (en)
EP (2) EP3629022A1 (en)
JP (4) JP5924937B2 (en)
KR (1) KR20110036638A (en)
CN (1) CN102177438A (en)
ES (1) ES2752025T3 (en)
WO (1) WO2010011944A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2777769C2 (en) * 2013-06-28 2022-08-09 Экс-Боди, Инк. Target antigen detection, phenotypic screening and its application for identification of specific target cell epitopes

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102177438A (en) * 2008-07-25 2011-09-07 理查德·W·瓦格纳 protein screening method
EP4335932A3 (en) 2008-11-07 2024-06-26 Adaptive Biotechnologies Corporation Methods of monitoring conditions by sequence analysis
US8628927B2 (en) 2008-11-07 2014-01-14 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
PL2387627T3 (en) 2009-01-15 2016-10-31 Adaptive immunity profiling and methods for generation of monoclonal antibodies
AP3396A (en) 2009-02-13 2015-08-31 Chem Inc X Methods of creating and screening DNA-encoded libraries
JP2012531202A (en) 2009-06-25 2012-12-10 フレッド ハチンソン キャンサー リサーチ センター How to measure adaptive immunity
US9880160B2 (en) 2011-03-15 2018-01-30 X-Body, Inc. Antibody screening methods
DK2748357T3 (en) 2011-09-07 2018-07-16 X Chem Inc Methods for labeling DNA-encoded libraries
US10385475B2 (en) 2011-09-12 2019-08-20 Adaptive Biotechnologies Corp. Random array sequencing of low-complexity libraries
AU2012347972B2 (en) 2011-12-05 2018-05-10 X-Body, Inc. PDGF receptor beta binding polypeptides
ES2662128T3 (en) 2012-03-05 2018-04-05 Adaptive Biotechnologies Corporation Determination of paired immune receptor chains from the frequency of matching subunits
MX354329B (en) 2012-05-08 2018-02-27 Adaptive Biotechnologies Corp Compositions and method for measuring and calibrating amplification bias in multiplexed pcr reactions.
IL236633B (en) 2012-07-13 2022-07-01 X Chem Inc Dna-encoded libraries comprising a complex having a cross-linking oligonucleotide linkage
US20140128275A1 (en) * 2012-08-21 2014-05-08 Syndecion, LLC Complex of non-covalently bound protein with encoding nucleic acids and uses thereof
US20160002731A1 (en) 2012-10-01 2016-01-07 Adaptive Biotechnologies Corporation Immunocompetence assessment by adaptive immune receptor diversity and clonality characterization
WO2015160439A2 (en) 2014-04-17 2015-10-22 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
JPWO2014119600A1 (en) * 2013-01-30 2017-01-26 ペプチドリーム株式会社 Flexible display method
WO2014142020A1 (en) * 2013-03-13 2014-09-18 国立大学法人東京大学 Nucleic acid linker
MY191368A (en) * 2013-06-28 2022-06-20 X Body Inc Target antigen discovery, phenotypic screens and use thereof for identification of target cell specific target epitopes
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
EP3049811B1 (en) 2013-09-23 2020-09-09 X-Body, Inc. Methods and compositions for generation of binding agents against cell surface antigens
US11390921B2 (en) 2014-04-01 2022-07-19 Adaptive Biotechnologies Corporation Determining WT-1 specific T cells and WT-1 specific T cell receptors (TCRs)
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
TWI751102B (en) 2014-08-28 2022-01-01 美商奇諾治療有限公司 Antibodies and chimeric antigen receptors specific for cd19
ES2784343T3 (en) 2014-10-29 2020-09-24 Adaptive Biotechnologies Corp Simultaneous, highly multiplexed detection of nucleic acids encoding paired adaptive immune receptor heterodimers from many samples
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
EP3498866A1 (en) 2014-11-25 2019-06-19 Adaptive Biotechnologies Corp. Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing
AU2016222788B2 (en) 2015-02-24 2022-03-31 Adaptive Biotechnologies Corp. Methods for diagnosing infectious disease and determining HLA status using immune repertoire sequencing
EP3277294B1 (en) 2015-04-01 2024-05-15 Adaptive Biotechnologies Corp. Method of identifying human compatible t cell receptors specific for an antigenic target
CA3024875A1 (en) 2016-06-16 2017-12-21 Richard Edward Watts Oligonucleotide directed and recorded combinatorial synthesis of encoded probe molecules
MA45491A (en) 2016-06-27 2019-05-01 Juno Therapeutics Inc CMH-E RESTRICTED EPITOPES, BINDING MOLECULES AND RELATED METHODS AND USES
CA3028002A1 (en) 2016-06-27 2018-01-04 Juno Therapeutics, Inc. Method of identifying peptide epitopes, molecules that bind such epitopes and related uses
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
US10738338B2 (en) 2016-10-18 2020-08-11 The Research Foundation for the State University Method and composition for biocatalytic protein-oligonucleotide conjugation and protein-oligonucleotide conjugate
JP7309197B2 (en) * 2017-03-29 2023-07-18 イッサム・リサーチ・ディベロップメント・カンパニー・オブ・ザ・ヘブルー・ユニバーシティ・オブ・エルサレム・リミテッド Purification of agents of interest
US11795580B2 (en) 2017-05-02 2023-10-24 Haystack Sciences Corporation Molecules for verifying oligonucleotide directed combinatorial synthesis and methods of making and using the same
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
JP7416715B2 (en) * 2018-11-07 2024-01-17 公益財団法人川崎市産業振興財団 Peptide-nucleic acid complex
WO2020205426A1 (en) * 2019-03-29 2020-10-08 The Regents Of The University Of California Comprehensive identification of interacting protein targets using mrna display of uniform libraries
KR102376443B1 (en) 2020-02-26 2022-03-17 포항공과대학교 산학협력단 A nanostructure, a biosensor including the nanostructure, and a screening method
EP4114943A4 (en) * 2020-03-05 2024-04-24 The Trustees Of Columbia University In The City Of New York In vivo mrna display: large-scale proteomics by next generation sequencing

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000032823A1 (en) * 1998-12-02 2000-06-08 Phylos, Inc. Dna-protein fusions and uses thereof
WO2001007657A1 (en) * 1999-07-27 2001-02-01 Phylos, Inc. Peptide acceptor ligation methods
US20030022236A1 (en) * 1997-01-21 2003-01-30 Szostak Jack W. Selection of proteins using RNA-protein fusions

Family Cites Families (138)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1311003A (en) 1919-07-22 Planooraph co
US40307A (en) 1863-10-13 Improvement in steam-engines
US1316003A (en) 1919-09-16 William clair toll
US700501A (en) 1901-06-17 1902-05-20 Willard C James Apartment-house.
US2223603A (en) 1938-07-15 1940-12-03 William A Darrah Equipment for heat treating
US2719403A (en) 1951-07-14 1955-10-04 Gisiger Armin Tight container
US3983001A (en) 1972-05-10 1976-09-28 Ceskoslovenska Akademie Ved Isolation of biologically active compounds by affinity chromatography
US4213860A (en) 1976-06-30 1980-07-22 Board of Regents, State of Florida for and on behalf of the University of Florida Affinity chromatography and substrate useful therefor
GB1602432A (en) 1976-12-15 1981-11-11 Atomic Energy Authority Uk Affinity chromatography
US4263428A (en) 1978-03-24 1981-04-21 The Regents Of The University Of California Bis-anthracycline nucleic acid function inhibitors and improved method for administering the same
US4175182A (en) 1978-07-03 1979-11-20 Research Corporation Separation of high-activity heparin by affinity chromatography on supported protamine
ATE12348T1 (en) 1980-11-10 1985-04-15 Gersonde Klaus Prof Dr PROCESS FOR THE PRODUCTION OF LIPID VESICLES BY ULTRASONIC TREATMENT, APPLICATION OF THE PROCESS AND DEVICE FOR CARRYING OUT THE PROCESS.
US4475196A (en) 1981-03-06 1984-10-02 Zor Clair G Instrument for locating faults in aircraft passenger reading light and attendant call control system
US4447233A (en) 1981-04-10 1984-05-08 Parker-Hannifin Corporation Medication infusion pump
DE3271828D1 (en) 1981-04-27 1986-07-31 Health Lab Service Board High pressure liquid affinity chromatography
EP0064378B1 (en) 1981-04-27 1987-02-11 The Public Health Laboratory Service Board Affinity chromatography using metal ions
US4485045A (en) 1981-07-06 1984-11-27 Research Corporation Synthetic phosphatidyl cholines useful in forming liposomes
EP0088046B1 (en) 1982-02-17 1987-12-09 Ciba-Geigy Ag Lipids in the aqueous phase
US4439196A (en) 1982-03-18 1984-03-27 Merck & Co., Inc. Osmotic drug delivery system
EP0102324A3 (en) 1982-07-29 1984-11-07 Ciba-Geigy Ag Lipids and surfactants in an aqueous medium
US4447224A (en) 1982-09-20 1984-05-08 Infusaid Corporation Variable flow implantable infusion apparatus
US4487603A (en) 1982-11-26 1984-12-11 Cordis Corporation Implantable microinfusion pump system
US4486194A (en) 1983-06-08 1984-12-04 James Ferrara Therapeutic device for administering medicaments through the skin
US4544545A (en) 1983-06-20 1985-10-01 Trustees University Of Massachusetts Liposomes containing modified cholesterol for organ targeting
ATE159858T1 (en) 1983-09-26 1997-11-15 Ehrenfeld Udo AGENT AND PRODUCT FOR THE DIAGNOSIS AND THERAPY OF TUMORS AND FOR THE TREATMENT OF WEAKNESSES OF THE CELLULAR AND HUMORAL IMMUNE DEFENSE
EP0143949B1 (en) 1983-11-01 1988-10-12 TERUMO KABUSHIKI KAISHA trading as TERUMO CORPORATION Pharmaceutical composition containing urokinase
US5171534A (en) 1984-01-16 1992-12-15 California Institute Of Technology Automated DNA sequencing technique
US5821058A (en) 1984-01-16 1998-10-13 California Institute Of Technology Automated DNA sequencing technique
US4596556A (en) 1985-03-25 1986-06-24 Bioject, Inc. Hypodermic injection apparatus
US4811218A (en) 1986-06-02 1989-03-07 Applied Biosystems, Inc. Real time scanning electrophoresis apparatus for DNA sequencing
US4941880A (en) 1987-06-19 1990-07-17 Bioject, Inc. Pre-filled ampule and non-invasive hypodermic injection device assembly
US4790824A (en) 1987-06-19 1988-12-13 Bioject, Inc. Non-invasive hypodermic injection device
US5149625A (en) 1987-08-11 1992-09-22 President And Fellows Of Harvard College Multiplex analysis of DNA
SE8801070D0 (en) 1988-03-23 1988-03-23 Pharmacia Ab METHOD FOR IMMOBILIZING A DNA SEQUENCE ON A SOLID SUPPORT
US4962020A (en) 1988-07-12 1990-10-09 President And Fellows Of Harvard College DNA sequencing
US5075216A (en) 1988-09-23 1991-12-24 Cetus Corporation Methods for dna sequencing with thermus aquaticus dna polymerase
WO1990005144A1 (en) 1988-11-11 1990-05-17 Medical Research Council Single domain ligands, receptors comprising said ligands, methods for their production, and use of said ligands and receptors
US5043062A (en) 1989-02-21 1991-08-27 Eastman Kodak Company High performance affinity chromatography column comprising non-porous, nondisperse polymeric packing material
US6291158B1 (en) 1989-05-16 2001-09-18 Scripps Research Institute Method for tapping the immunological repertoire
ATE168416T1 (en) 1989-10-05 1998-08-15 Optein Inc CELL-FREE SYNTHESIS AND ISOLATION OF GENES AND POLYPEPTIDES
US5064413A (en) 1989-11-09 1991-11-12 Bioject, Inc. Needleless hypodermic injection device
US5312335A (en) 1989-11-09 1994-05-17 Bioject Inc. Needleless hypodermic injection device
ATE126535T1 (en) 1990-04-05 1995-09-15 Roberto Crea ''WALK-THROUGH'' MUTAGENesis.
GB9015198D0 (en) 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
US6172197B1 (en) 1991-07-10 2001-01-09 Medical Research Council Methods for producing members of specific binding pairs
US5843701A (en) 1990-08-02 1998-12-01 Nexstar Pharmaceticals, Inc. Systematic polypeptide evolution by reverse translation
US5846951A (en) 1991-06-06 1998-12-08 The School Of Pharmacy, University Of London Pharmaceutical compositions
CA2124460C (en) 1991-12-02 2007-08-28 Andrew David Griffiths Production of anti-self antibodies from segment repertoires and displayed on phage
DE69233782D1 (en) 1991-12-02 2010-05-20 Medical Res Council Preparation of Autoantibodies on Phage Surfaces Starting from Antibody Segment Libraries
GB9208733D0 (en) 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
US5383851A (en) 1992-07-24 1995-01-24 Bioject Inc. Needleless hypodermic injection device
US6765087B1 (en) 1992-08-21 2004-07-20 Vrije Universiteit Brussel Immunoglobulins devoid of light chains
ATE267877T1 (en) 1993-01-07 2004-06-15 Sequenom Inc DNA SEQUENCING THROUGH MASS SPECTRONOMY
US5374527A (en) 1993-01-21 1994-12-20 Applied Biosystems, Inc. High resolution DNA sequencing method using low viscosity medium
IL108497A0 (en) 1993-02-01 1994-05-30 Seq Ltd Methods and apparatus for dna sequencing
DE69430909T2 (en) 1993-03-19 2003-02-27 Sequenom, Inc. DNA SEQUENCE DETERMINATION BY MASS SPECTROMETRY ON THE WAY OF DEGRADATION WITH EXONUCLEASE
US6838254B1 (en) 1993-04-29 2005-01-04 Conopco, Inc. Production of antibodies or (functionalized) fragments thereof derived from heavy chain immunoglobulins of camelidae
US5922545A (en) 1993-10-29 1999-07-13 Affymax Technologies N.V. In vitro peptide and antibody display libraries
SE9400088D0 (en) 1994-01-14 1994-01-14 Kabi Pharmacia Ab Bacterial receptor structures
US5654419A (en) 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
UA71889C2 (en) 1996-04-02 2005-01-17 Йєда Рісерч Енд Дівелопмент Ко. Лтд. Modulators of tnf factor (traf) related to receptor, preparation and use thereof
US5846727A (en) 1996-06-06 1998-12-08 Board Of Supervisors Of Louisiana State University And Agricultural & Mechanical College Microsystem for rapid DNA sequencing
DE69730157T2 (en) 1996-10-17 2005-07-28 Mitsubishi Chemical Corp. MOLECULE, WHICH GENOTYP AND PHENOTYPE COMBINED AND ITS APPLICATIONS
US5858671A (en) 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US6258533B1 (en) 1996-11-01 2001-07-10 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US5876934A (en) 1996-12-18 1999-03-02 Pharmacia Biotech Inc. DNA sequencing method
AU776478B2 (en) 1997-01-21 2004-09-09 General Hospital Corporation, The Selection of proteins using RNA-protein fusions
RU2233878C2 (en) 1997-01-21 2004-08-10 Дзе Дженерал Хоспитал Корпорейшн Method for selection of desirable protein and nucleic acid, agents for its realization
GB9703369D0 (en) 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
US6090800A (en) * 1997-05-06 2000-07-18 Imarx Pharmaceutical Corp. Lipid soluble steroid prodrugs
ATE321849T1 (en) 1997-04-23 2006-04-15 Univ Zuerich METHOD FOR DETECTING NUCLEIC ACID MOLECULES CODING FOR (POLY)PEPTIDES WHICH INTERACT WITH TARGET MOLECULES
JP4060892B2 (en) 1997-05-28 2008-03-12 ディサーナ リミテッド Ribosome complexes as selective particles for in vitro display and evolution of proteins
DE19742706B4 (en) 1997-09-26 2013-07-25 Pieris Proteolab Ag lipocalin muteins
GB9722131D0 (en) 1997-10-20 1997-12-17 Medical Res Council Method
US6153383A (en) * 1997-12-09 2000-11-28 Verdine; Gregory L. Synthetic transcriptional modulators and uses thereof
WO1999031700A1 (en) 1997-12-15 1999-06-24 E.I. Du Pont De Nemours And Company Ion-bombarded graphite electron emitters
JP2002510505A (en) 1998-04-03 2002-04-09 フィロス インク． Localizable protein arrays
US6143503A (en) 1998-04-17 2000-11-07 Whitehead Institute For Biomedical Research Use of a ribozyme to join nucleic acids and peptides
US6846655B1 (en) 1998-06-29 2005-01-25 Phylos, Inc. Methods for generating highly diverse libraries
WO2000009741A1 (en) * 1998-08-13 2000-02-24 The Trustees Of The University Of Pennsylvania Method of identifying proteins
US6312927B1 (en) 1998-08-17 2001-11-06 Phylos, Inc. Methods for producing nucleic acids lacking 3'-untranslated regions and optimizing cellular RNA-protein fusion formation
PT1105360E (en) 1998-08-17 2009-09-30 Bristol Myers Squibb Co Identification of compound-protein interactions using libraries of protein-nucleic acid fusion molecules
US7115396B2 (en) 1998-12-10 2006-10-03 Compound Therapeutics, Inc. Protein scaffolds for antibody mimics and other binding proteins
US6818418B1 (en) 1998-12-10 2004-11-16 Compound Therapeutics, Inc. Protein scaffolds for antibody mimics and other binding proteins
US6413720B1 (en) * 1999-01-11 2002-07-02 Signal Pharmaceuticals, Inc. Method for the affinity isolation of newly synthesized RNA
EP1187626A4 (en) 1999-06-01 2006-07-19 Compound Therapeutics Inc Methods for producing 5'-nucleic acid-protein conjugates
WO2001004265A2 (en) 1999-07-12 2001-01-18 Phylos, Inc. C-terminal protein tagging
CA2382545C (en) 1999-08-27 2013-08-13 Phylos, Inc. Methods for encoding and sorting in vitro translated proteins
WO2001016600A1 (en) 1999-08-31 2001-03-08 Mitsubishi Chemical Corporation Method of analyzing mutual interaction between protein and molecule
US20010046680A1 (en) 1999-10-01 2001-11-29 Zhongping Yu Identification of polypeptides and nucleic acid molecules using linkage between DNA and polypeptide
US6794132B2 (en) 1999-10-02 2004-09-21 Biosite, Inc. Human antibodies
US7022479B2 (en) 2000-01-24 2006-04-04 Compound Therapeutics, Inc. Sensitive, multiplexed diagnostic assays for protein analysis
WO2001053539A1 (en) 2000-01-24 2001-07-26 Phylos, Inc. Sensitive, multiplexed diagnostic assays for protein analysis
JP2003529369A (en) 2000-03-31 2003-10-07 ケンブリッジ アンティボディー テクノロジー リミティド Improved ribosome display
AU2001253410A1 (en) 2000-04-12 2001-10-30 Genaissance Pharmaceuticals, Inc. Haplotypes of the il8rb gene
EP1283897A4 (en) 2000-05-16 2004-08-04 Merck & Co Inc GENE RESPONSIBLE FOR STARGARDT'S DOMINANT MACULAR DYSTROPHY
CA2421447C (en) 2000-09-08 2012-05-08 Universitat Zurich Collections of repeat proteins comprising repeat modules
JP3750020B2 (en) * 2000-12-07 2006-03-01 学校法人慶應義塾 C-terminal modified protein and production method thereof, modifying agent and translation template used for production of C-terminal modified protein, and protein interaction detection method using C-terminal modified protein
JP4963142B2 (en) * 2000-12-14 2012-06-27 学校法人慶應義塾 Genotype-phenotype mapping molecule and its constituents, and method for producing and using mapping molecule
US20030133939A1 (en) 2001-01-17 2003-07-17 Genecraft, Inc. Binding domain-immunoglobulin fusion proteins
WO2002088171A2 (en) 2001-04-26 2002-11-07 Avidia Research Institute Combinatorial libraries of monomer domains
US20050089932A1 (en) 2001-04-26 2005-04-28 Avidia Research Institute Novel proteins with targeted binding
US20050048512A1 (en) 2001-04-26 2005-03-03 Avidia Research Institute Combinatorial libraries of monomer domains
US20030157561A1 (en) 2001-11-19 2003-08-21 Kolkman Joost A. Combinatorial libraries of monomer domains
US20040175756A1 (en) 2001-04-26 2004-09-09 Avidia Research Institute Methods for using combinatorial libraries of monomer domains
US20050053973A1 (en) 2001-04-26 2005-03-10 Avidia Research Institute Novel proteins with targeted binding
US20060223114A1 (en) 2001-04-26 2006-10-05 Avidia Research Institute Protein scaffolds and uses thereof
WO2002101036A1 (en) 2001-05-11 2002-12-19 Kazunari Taira Method of forming stable complex of transcription product of dna encoding arbitrary peptide with translation product, nucleic acid construct to be used in the method, complex formed by the method, and screening of functional protein and mrna or dna encoding the protein using the method
WO2003000856A2 (en) 2001-06-21 2003-01-03 Phylos, Inc. In vitro protein interaction detection systems
DE60237282D1 (en) 2001-06-28 2010-09-23 Domantis Ltd DOUBLE-SPECIFIC LIGAND AND ITS USE
JP2004537312A (en) 2001-07-31 2004-12-16 フィロス インク． Modular assembly of nucleic acid-protein fusion multimers
US7125669B2 (en) 2001-11-27 2006-10-24 Compound Therapeutics, Inc. Solid-phase immobilization of proteins and peptides
US20060002935A1 (en) * 2002-06-28 2006-01-05 Domantis Limited Tumor Necrosis Factor Receptor 1 antagonists and methods of use therefor
EP1600459A3 (en) 2002-06-28 2005-12-07 Domantis Limited Ligand
US20040043384A1 (en) * 2002-08-28 2004-03-04 Oleinikov Andrew V. In vitro protein translation microarray device
DK1534830T3 (en) 2002-09-06 2011-02-14 Isogenica Ltd A vito-peptide expression library
US20040259155A1 (en) 2002-09-30 2004-12-23 Compound Therapeutics, Inc. Methods of engineering spatially conserved motifs in polypeptides
AU2003286004A1 (en) 2002-11-08 2004-06-07 Ablynx N.V. Single domain antibodies directed against interferon- gamma and uses therefor
JP4478772B2 (en) * 2002-12-11 2010-06-09 学校法人慶應義塾 Protein that forms a complex with c-Fos protein, nucleic acid encoding the same, and methods for using them
CA2511910A1 (en) 2002-12-27 2004-07-15 Domantis Limited Dual specific single domain antibodies specific for a ligand and for the receptor of the ligand
ES2326649T3 (en) 2003-03-19 2009-10-16 Crescendo Biologics Ltd. DNA RECOVERY.
CA2525120C (en) 2003-05-14 2013-04-30 Domantis Limited A process for recovering polypeptides that unfold reversibly from a polypeptide repertoire
AU2004220325B2 (en) 2003-06-30 2011-05-12 Domantis Limited Polypeptides
ES2294535T3 (en) 2003-08-12 2008-04-01 Lipoxen Technologies Limited DERIVATIVES OF POLISIALIC ACID.
WO2005030957A1 (en) 2003-10-01 2005-04-07 ETH Zürich Method for in vitro evolution of polypeptides
EP1675878A2 (en) 2003-10-24 2006-07-05 Avidia, Inc. Ldl receptor class a and egf domain monomers and multimers
US20060008844A1 (en) 2004-06-17 2006-01-12 Avidia Research Institute c-Met kinase binding proteins
US20060234299A1 (en) 2004-11-16 2006-10-19 Avidia Research Institute Protein scaffolds and uses thereof
JP2008528010A (en) 2005-01-31 2008-07-31 アブリンクス ナームローゼ フェンノートシャップ Methods for creating variable domain sequences of heavy chain antibodies
WO2006081623A1 (en) * 2005-02-04 2006-08-10 The Australian National University Double-stranded oligonucleotides and uses therefor
JP2008531764A (en) 2005-02-23 2008-08-14 リポクセン テクノロジーズ リミテッド Activated sialic acid derivatives for derivatization and binding of proteins
JP2009509535A (en) 2005-09-27 2009-03-12 アムニクス， インコーポレイテッド Proteinaceous drugs and their use
WO2007059782A1 (en) 2005-11-28 2007-05-31 Genmab A/S Recombinant monovalent antibodies and methods for production thereof
JP4616237B2 (en) 2006-11-07 2011-01-19 日本電信電話株式会社 Method for forming silicon compound thin film
US8236508B2 (en) 2008-01-29 2012-08-07 Drexel University Detecting and measuring live pathogens utilizing a mass detection device
CN102177438A (en) * 2008-07-25 2011-09-07 理查德·W·瓦格纳 protein screening method
US8698004B2 (en) 2008-10-27 2014-04-15 Ibiden Co., Ltd. Multi-piece board and fabrication method thereof
US8876866B2 (en) 2010-12-13 2014-11-04 Globus Medical, Inc. Spinous process fusion devices and methods thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030022236A1 (en) * 1997-01-21 2003-01-30 Szostak Jack W. Selection of proteins using RNA-protein fusions
WO2000032823A1 (en) * 1998-12-02 2000-06-08 Phylos, Inc. Dna-protein fusions and uses thereof
WO2001007657A1 (en) * 1999-07-27 2001-02-01 Phylos, Inc. Peptide acceptor ligation methods

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
J. E. BARRICK ET AL.: "Selection of RNA-binding peptides using mRNA-peptide fusions.", METHODS., vol. 23, 2001, pages 287 - 293, XP008142721 *
M. BIYANI ET AL.: "Solid-phase translation and RNA-protein fusion: a novel approach for folding quality control and direct immobilization of proteins using anchored mRNA.", NUCLEIC ACIDS RESEARCH, vol. 34, no. 20, 24 October 2006 (2006-10-24), pages E140, XP002576823 *
M. YONEZAWA ET AL.: "DNA display of biologically active proteins for in vitro protein selection.", J. BIOCHEM., vol. 135, 2004, pages 285 - 288, XP002991790 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2777769C2 (en) * 2013-06-28 2022-08-09 Экс-Боди, Инк. Target antigen detection, phenotypic screening and its application for identification of specific target cell epitopes

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