WO2009147455A2 - Method of differentiated development of genetic cells in a substratum autologous - heterologous targeted fixation cryopreservation bank of regenerated fluid tissues - tissues - Google Patents
Method of differentiated development of genetic cells in a substratum autologous - heterologous targeted fixation cryopreservation bank of regenerated fluid tissues - tissues Download PDFInfo
- Publication number
- WO2009147455A2 WO2009147455A2 PCT/GR2009/000036 GR2009000036W WO2009147455A2 WO 2009147455 A2 WO2009147455 A2 WO 2009147455A2 GR 2009000036 W GR2009000036 W GR 2009000036W WO 2009147455 A2 WO2009147455 A2 WO 2009147455A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- tissues
- cells
- heterologous
- autologous
- substratum
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 10
- 238000005138 cryopreservation Methods 0.000 title claims description 6
- 238000011161 development Methods 0.000 title description 4
- 230000002068 genetic effect Effects 0.000 title description 4
- 239000012530 fluid Substances 0.000 title description 2
- 239000003102 growth factor Substances 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 claims description 2
- 230000001149 cognitive effect Effects 0.000 claims 1
- 230000009916 joint effect Effects 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 14
- 210000001519 tissue Anatomy 0.000 description 9
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 210000000056 organ Anatomy 0.000 description 3
- 239000000427 antigen Substances 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000002054 transplantation Methods 0.000 description 2
- 108090000695 Cytokines Proteins 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 102000002274 Matrix Metalloproteinases Human genes 0.000 description 1
- 108010000684 Matrix Metalloproteinases Proteins 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000019552 anatomical structure morphogenesis Effects 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000004489 deciduous teeth Anatomy 0.000 description 1
- 210000002308 embryonic cell Anatomy 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 230000013632 homeostatic process Effects 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 102000006495 integrins Human genes 0.000 description 1
- 108010044426 integrins Proteins 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000003032 molecular docking Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000002611 ovarian Effects 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 210000004976 peripheral blood cell Anatomy 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 108091035539 telomere Proteins 0.000 description 1
- 102000055501 telomere Human genes 0.000 description 1
- 210000003411 telomere Anatomy 0.000 description 1
- 230000002381 testicular Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001550 testis Anatomy 0.000 description 1
- 239000000724 thymus hormone Substances 0.000 description 1
- 230000025366 tissue development Effects 0.000 description 1
- 230000017423 tissue regeneration Effects 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
Definitions
- METHOD Genetic cells collected by aspiration (ovaries), or biopsy (testes), are cultured (proliferated / differentiated / maturing) in a cell-culture, with addition of FSH, LH, etc., of internationally known secondary sex-linked hormones and growth factors, such as GH, rhlGF-1, II-2 thymus hormones, etc., in a matrix of umbilical blood cells or peripheral blood cells following stimulation with growth factors and collection with cytokines either following collection through amnioparacentisis or from a cryopreservation bank of umbilicoplacental blood, or from deciduous teeth - with concurrent presence of a homologous or heterologous element [in which case the heterologous element is properly washed by surface antigens (the sequence of processing is known)], either through the established procedure or successive baths into umbilicoplacental blood [our own method] , testicular/ovarian tissue, or cells aspirated by needle.
- FSH secondary sex-linked hormones
- the whole set is supplied by microproteins from the environment of the organ [i.e. creation of a designer microenvironment] and use is made of a 3D- reconstructed basal membrane in order to obtain full expression of the tissue specialization, in a totally excellent achievement of consistency and tissue maturity, as well as of perfect homeostasis in the bioculture.
- the result will be the synthesis of extracellular matrix, the rate of integrins, other (any) receptors on the surface of the cells, MMPs, as well as the rate of their inhibitors in order to optimize the (tissue-specialized) expression of genes, to complete in a fully natural way their development, their morphogenesis (which is the goal of this project) and obtain their normal general behaviour.
Landscapes
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GR20080100392A GR1007146B (el) | 2008-06-05 | 2008-06-05 | Μεθοδος διαφοροποιητικης αναπτυξης σε υποστρωμα, γεννετικων κυτταρων-αυτολογη-ετερολογη στοχευμενη αγκιστρωση, τραπεζα κρυοδιατηρησης αναγεννημενων υγρων ιστων-ιστων |
| GR20080100392 | 2008-06-05 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2009147455A2 true WO2009147455A2 (en) | 2009-12-10 |
Family
ID=40942524
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/GR2009/000036 WO2009147455A2 (en) | 2008-06-05 | 2009-06-05 | Method of differentiated development of genetic cells in a substratum autologous - heterologous targeted fixation cryopreservation bank of regenerated fluid tissues - tissues |
Country Status (2)
| Country | Link |
|---|---|
| GR (1) | GR1007146B (el) |
| WO (1) | WO2009147455A2 (el) |
-
2008
- 2008-06-05 GR GR20080100392A patent/GR1007146B/el not_active IP Right Cessation
-
2009
- 2009-06-05 WO PCT/GR2009/000036 patent/WO2009147455A2/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| GR1007146B (el) | 2011-01-14 |
| GR20080100392A (el) | 2010-01-27 |
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