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WO2006112713A3 - High throughput screening method for assessing heterogeneity of microorganisms - Google Patents

High throughput screening method for assessing heterogeneity of microorganisms Download PDF

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Publication number
WO2006112713A3
WO2006112713A3 PCT/NL2006/050087 NL2006050087W WO2006112713A3 WO 2006112713 A3 WO2006112713 A3 WO 2006112713A3 NL 2006050087 W NL2006050087 W NL 2006050087W WO 2006112713 A3 WO2006112713 A3 WO 2006112713A3
Authority
WO
WIPO (PCT)
Prior art keywords
high throughput
microorganisms
throughput screening
screening method
heterogeneity
Prior art date
Application number
PCT/NL2006/050087
Other languages
French (fr)
Other versions
WO2006112713A2 (en
Inventor
Colin Ingham
Vos Willem Meindert De
Hylckama Vlieg Johan Van
Johan Gerrit Bomer
Adrianus Joseph Sprenkels
Den Berg Albert Van
Original Assignee
Stichting Technologisch Top In
Colin Ingham
Vos Willem Meindert De
Hylckama Vlieg Johan Van
Johan Gerrit Bomer
Adrianus Joseph Sprenkels
Den Berg Albert Van
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/NL2006/050078 external-priority patent/WO2006112709A2/en
Application filed by Stichting Technologisch Top In, Colin Ingham, Vos Willem Meindert De, Hylckama Vlieg Johan Van, Johan Gerrit Bomer, Adrianus Joseph Sprenkels, Den Berg Albert Van filed Critical Stichting Technologisch Top In
Priority to US11/918,612 priority Critical patent/US20100021959A1/en
Priority to EP06733066A priority patent/EP1871893A2/en
Publication of WO2006112713A2 publication Critical patent/WO2006112713A2/en
Publication of WO2006112713A3 publication Critical patent/WO2006112713A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00427—Means for dispensing and evacuation of reagents using masks
    • B01J2219/00432—Photolithographic masks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/0054—Means for coding or tagging the apparatus or the reagents
    • B01J2219/00572—Chemical means
    • B01J2219/00576—Chemical means fluorophore
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/0061—The surface being organic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00614—Delimitation of the attachment areas
    • B01J2219/00621—Delimitation of the attachment areas by physical means, e.g. trenches, raised areas
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00639—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium
    • B01J2219/00641—Making arrays on substantially continuous surfaces the compounds being trapped in or bound to a porous medium the porous medium being continuous, e.g. porous oxide substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/0074—Biological products
    • B01J2219/00743—Cells

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to the field of microbiology. Provided is a method which is particularly powerful for High Throughput Screening (HTS) purposes. More specific a high throughput method for determining heterogeneity or interactions of microorganisms is provided.
PCT/NL2006/050087 2005-04-18 2006-04-18 High throughput screening method for assessing heterogeneity of microorganisms WO2006112713A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US11/918,612 US20100021959A1 (en) 2005-04-18 2006-04-18 High Throughput Screening Method for Assessing Heterogeneity of Microorganisms
EP06733066A EP1871893A2 (en) 2005-04-18 2006-04-18 High throughput screening method for assessing heterogeneity of microorganisms

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
EP05103080.7 2005-04-18
EP05103080 2005-04-18
US69650705P 2005-07-06 2005-07-06
US60/696,507 2005-07-06
PCT/NL2006/050078 WO2006112709A2 (en) 2005-04-18 2006-04-10 Biochip and process for the production of a biochip
NLPCT/NL2006/050078 2006-04-10

Publications (2)

Publication Number Publication Date
WO2006112713A2 WO2006112713A2 (en) 2006-10-26
WO2006112713A3 true WO2006112713A3 (en) 2007-05-10

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL2006/050087 WO2006112713A2 (en) 2005-04-18 2006-04-18 High throughput screening method for assessing heterogeneity of microorganisms

Country Status (1)

Country Link
WO (1) WO2006112713A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102735517B (en) * 2012-06-29 2014-09-03 成都欧林生物科技股份有限公司 Dyeing method for bacterial capsules in Haemophilus influenzae type b fermentation broth

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008153387A1 (en) * 2007-06-12 2008-12-18 Nizo Food Research B.V. Method for high-throughput cheese making
US8011133B2 (en) 2007-06-27 2011-09-06 Pioneer Hi-Bred International, Inc. Method and apparatus of high-throughput pollen extraction, counting, and use of counted pollen for characterizing a plant
EP2223179A1 (en) * 2007-12-19 2010-09-01 DSM IP Assets B.V. Method for taking a plurality of samples
GB2476663A (en) * 2009-12-31 2011-07-06 Blood Analysis Ltd Detection of microorganisms
WO2012129199A2 (en) * 2011-03-22 2012-09-27 Pioneer Hi-Bred International, Inc. Apparatuses and methods for evaluating and sorting pollen and plants
CN111139281A (en) * 2020-01-13 2020-05-12 华南农业大学 Method for accurately measuring special-state bacteria based on microfluidic visualization technology and sorting and enriching special-state bacteria
FR3108983B1 (en) * 2020-04-03 2022-03-25 Commissariat Energie Atomique Method for characterizing microorganisms by transmission imaging

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4528269A (en) * 1980-04-14 1985-07-09 The State Of Oregon By And Through The Oregon State Board Of Higher Education On Behalf Of Oregon State University Method for producing single and/or mixed strain concentrates of bacteria
WO2003025113A2 (en) * 2001-09-14 2003-03-27 Hans-Knöll-Institut für Naturstoff-Forschung e.V. Methods for cultivating and analyzing microbial individual cell cultures
WO2003102578A2 (en) * 2002-06-03 2003-12-11 Pamgene B.V. High throughput cellular response assay using microarrays

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4528269A (en) * 1980-04-14 1985-07-09 The State Of Oregon By And Through The Oregon State Board Of Higher Education On Behalf Of Oregon State University Method for producing single and/or mixed strain concentrates of bacteria
WO2003025113A2 (en) * 2001-09-14 2003-03-27 Hans-Knöll-Institut für Naturstoff-Forschung e.V. Methods for cultivating and analyzing microbial individual cell cultures
WO2003102578A2 (en) * 2002-06-03 2003-12-11 Pamgene B.V. High throughput cellular response assay using microarrays

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BINNERUP SVEND JORGEN ET AL: "Gram characteristics determined on single cells and at the microcolony level of bacteria immobilized on polycarbonate membrane filters", JOURNAL OF MICROBIOLOGICAL METHODS, vol. 31, no. 3, January 1998 (1998-01-01), pages 185 - 192, XP002422029, ISSN: 0167-7012 *
HOJBERG OLE ET AL: "Growth of silicone-immobilized bacteria on polycarbonate membrane filters, a technique to study microcolony formation under anaerobic conditions", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 63, no. 7, 1997, pages 2920 - 2924, XP002422028, ISSN: 0099-2240 *
INGHAM COLIN J ET AL: "Growth and multiplexed analysis of microorganisms on a subdivided, highly porous, inorganic chip manufactured from anopore", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 71, no. 12, December 2005 (2005-12-01), pages 8978 - 8981, XP002422030, ISSN: 0099-2240 *
WILDERER PETER A ET AL: "Modern scientific methods and their potential in wastewater science and technology", WATER RESEARCH, ELSEVIER, AMSTERDAM, NL, vol. 36, no. 2, January 2002 (2002-01-01), pages 370 - 393, XP004323360, ISSN: 0043-1354 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102735517B (en) * 2012-06-29 2014-09-03 成都欧林生物科技股份有限公司 Dyeing method for bacterial capsules in Haemophilus influenzae type b fermentation broth

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