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WO2006017342A3 - Assay methods for enzymes associated with sulfation - Google Patents

Assay methods for enzymes associated with sulfation Download PDF

Info

Publication number
WO2006017342A3
WO2006017342A3 PCT/US2005/024875 US2005024875W WO2006017342A3 WO 2006017342 A3 WO2006017342 A3 WO 2006017342A3 US 2005024875 W US2005024875 W US 2005024875W WO 2006017342 A3 WO2006017342 A3 WO 2006017342A3
Authority
WO
WIPO (PCT)
Prior art keywords
sulfation
assay methods
methods
enzymes associated
activity
Prior art date
Application number
PCT/US2005/024875
Other languages
French (fr)
Other versions
WO2006017342A2 (en
Inventor
Sunil Bhakta
Stefan Hemmerich
Hoa Ly
Joe Lyssikatos
Dawn Verdugo
Original Assignee
Y S Therapeutics Inc
Sunil Bhakta
Stefan Hemmerich
Hoa Ly
Joe Lyssikatos
Dawn Verdugo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Y S Therapeutics Inc, Sunil Bhakta, Stefan Hemmerich, Hoa Ly, Joe Lyssikatos, Dawn Verdugo filed Critical Y S Therapeutics Inc
Publication of WO2006017342A2 publication Critical patent/WO2006017342A2/en
Publication of WO2006017342A3 publication Critical patent/WO2006017342A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention provides compositions, methods and kits for determining whether an agent modulates the activity of an enzyme associated with the sulfation of biological molecules, such as sulfotransferases and sulfatases. The methods of the present invention are advantageous in that they are amenable to high throughput production and quantitation. Test agents which enhance or inhibit the activity of an enzyme associated with sulfation of biological molecules may be identified by the methods described herein.
PCT/US2005/024875 2004-07-13 2005-07-12 Assay methods for enzymes associated with sulfation WO2006017342A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US58797504P 2004-07-13 2004-07-13
US60/587,975 2004-07-13

Publications (2)

Publication Number Publication Date
WO2006017342A2 WO2006017342A2 (en) 2006-02-16
WO2006017342A3 true WO2006017342A3 (en) 2007-12-27

Family

ID=35839808

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/024875 WO2006017342A2 (en) 2004-07-13 2005-07-12 Assay methods for enzymes associated with sulfation

Country Status (1)

Country Link
WO (1) WO2006017342A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012161688A1 (en) * 2011-05-23 2012-11-29 Research And Diagnostic Systems, Inc. Sulfotransferase assay
US8815529B2 (en) 2011-05-23 2014-08-26 Research & Diagnostics Systems, Inc. Sulfotransferase assay

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6017722A (en) * 1991-04-04 2000-01-25 Board Of Regents, The University Of Texas System Luminous bacteria and methods for the isolation, identification and quantitation of toxicants
US20030152518A1 (en) * 2001-12-21 2003-08-14 Threshold Pharmaceuticals, Inc. Methods for cancer imaging
US20030211562A1 (en) * 2002-05-13 2003-11-13 Carolyn Bertozzi Method for identifying modulators of sulfotransferase activity

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6017722A (en) * 1991-04-04 2000-01-25 Board Of Regents, The University Of Texas System Luminous bacteria and methods for the isolation, identification and quantitation of toxicants
US20030152518A1 (en) * 2001-12-21 2003-08-14 Threshold Pharmaceuticals, Inc. Methods for cancer imaging
US20030211562A1 (en) * 2002-05-13 2003-11-13 Carolyn Bertozzi Method for identifying modulators of sulfotransferase activity

Also Published As

Publication number Publication date
WO2006017342A2 (en) 2006-02-16

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