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WO2005113791A3 - Methods of identifying modulators of bacterial type iii protein secretion system - Google Patents

Methods of identifying modulators of bacterial type iii protein secretion system Download PDF

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Publication number
WO2005113791A3
WO2005113791A3 PCT/US2005/015809 US2005015809W WO2005113791A3 WO 2005113791 A3 WO2005113791 A3 WO 2005113791A3 US 2005015809 W US2005015809 W US 2005015809W WO 2005113791 A3 WO2005113791 A3 WO 2005113791A3
Authority
WO
WIPO (PCT)
Prior art keywords
type iii
protein secretion
secretion system
iii protein
methods
Prior art date
Application number
PCT/US2005/015809
Other languages
French (fr)
Other versions
WO2005113791A2 (en
Inventor
Raul Goldschmidt
Michael Loeloff
Original Assignee
Johnson & Johnson Product Res
Raul Goldschmidt
Michael Loeloff
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johnson & Johnson Product Res, Raul Goldschmidt, Michael Loeloff filed Critical Johnson & Johnson Product Res
Publication of WO2005113791A2 publication Critical patent/WO2005113791A2/en
Publication of WO2005113791A3 publication Critical patent/WO2005113791A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18—Testing for antimicrobial activity of a material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/978—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • G01N2333/986—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides (3.5.2), e.g. beta-lactamase (penicillinase, 3.5.2.6), creatinine amidohydrolase (creatininase, EC 3.5.2.10), N-methylhydantoinase (3.5.2.6)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention provides methods to identify inhibitors or activators of bacterial type III protein secretion system using a recombinant β-lactamase that can be secreted by a type III protein secretion system. Such an assay can be easily adopted in a high thoroughput mode that allows the daily screening of several tens of thousands compounds. Inhibitors for the Salmonella typhimurium SPI-1 type III protein secretion system have been identified in applying methods of the invention. These inhibitors were shown to be active against the type III protein secretion system of other Gram-negative bacteria, such as Pseudomonas aeruginosa. The compounds identified by the methods of the invention can be used as new antimicrobial therapeutics.
PCT/US2005/015809 2004-05-07 2005-05-06 Methods of identifying modulators of bacterial type iii protein secretion system WO2005113791A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US56901304P 2004-05-07 2004-05-07
US60/569,013 2004-05-07

Publications (2)

Publication Number Publication Date
WO2005113791A2 WO2005113791A2 (en) 2005-12-01
WO2005113791A3 true WO2005113791A3 (en) 2006-08-10

Family

ID=35428932

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/015809 WO2005113791A2 (en) 2004-05-07 2005-05-06 Methods of identifying modulators of bacterial type iii protein secretion system

Country Status (1)

Country Link
WO (1) WO2005113791A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2550366T3 (en) 2010-03-23 2014-12-15 Imba Inst Für Molekulare Biotechnologie Gmbh Methods for identifying inhibitors of the type III secretion system

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5348867A (en) * 1991-11-15 1994-09-20 George Georgiou Expression of proteins on bacterial surface
US5928888A (en) * 1996-09-26 1999-07-27 Aurora Biosciences Corporation Methods and compositions for sensitive and rapid, functional identification of genomic polynucleotides and secondary screening capabilities
WO1999045136A1 (en) * 1998-03-05 1999-09-10 University Of British Columbia Methods for assaying type iii secretion inhibitors
WO1999058714A2 (en) * 1998-05-13 1999-11-18 Institut Pasteur Method for screening for inhibitors and activators of type iii secretion machinery in gram-negative bacteria
US20020192740A1 (en) * 2001-01-22 2002-12-19 Satoshi Omura Method for detecting substances inhibiting the bacterial type III secretion mechanism and function of secretory proteins thereof
US20030045702A1 (en) * 2000-12-14 2003-03-06 Institut Pasteur Secreted Chlamydia polypeptides and method for identifying such polypeptides by their secretion by a type III secretion pathway of a gram-negative bacteria

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5348867A (en) * 1991-11-15 1994-09-20 George Georgiou Expression of proteins on bacterial surface
US5928888A (en) * 1996-09-26 1999-07-27 Aurora Biosciences Corporation Methods and compositions for sensitive and rapid, functional identification of genomic polynucleotides and secondary screening capabilities
WO1999045136A1 (en) * 1998-03-05 1999-09-10 University Of British Columbia Methods for assaying type iii secretion inhibitors
WO1999058714A2 (en) * 1998-05-13 1999-11-18 Institut Pasteur Method for screening for inhibitors and activators of type iii secretion machinery in gram-negative bacteria
US6136542A (en) * 1998-05-13 2000-10-24 Institut Pasteur Method for screening for inhibitors and activators of type III secretion machinery in gram-negative bacteria
US20030045702A1 (en) * 2000-12-14 2003-03-06 Institut Pasteur Secreted Chlamydia polypeptides and method for identifying such polypeptides by their secretion by a type III secretion pathway of a gram-negative bacteria
US20020192740A1 (en) * 2001-01-22 2002-12-19 Satoshi Omura Method for detecting substances inhibiting the bacterial type III secretion mechanism and function of secretory proteins thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Goldschmidt R. et al., 'Identification and characterization of inhibitors of bacterial type III protein secretion systems (TTPS) as potential antimicrobial agents.' Abstracts of the 44th Interscience Conference on Antimicrobial Agents and Chemotherapy, Oct-Nov 2004, vol. 44, p. 201, Abst. F-712. *

Also Published As

Publication number Publication date
WO2005113791A2 (en) 2005-12-01

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