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WO2005037850A3 - Solid phase methods for polynucleotide production - Google Patents

Solid phase methods for polynucleotide production Download PDF

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Publication number
WO2005037850A3
WO2005037850A3 PCT/US2004/033919 US2004033919W WO2005037850A3 WO 2005037850 A3 WO2005037850 A3 WO 2005037850A3 US 2004033919 W US2004033919 W US 2004033919W WO 2005037850 A3 WO2005037850 A3 WO 2005037850A3
Authority
WO
WIPO (PCT)
Prior art keywords
oligonucleotide
solid support
target polynucleotide
solid phase
annealed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2004/033919
Other languages
French (fr)
Other versions
WO2005037850A2 (en
Inventor
Hsing-Yeh Parker
John T Mulligan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Blue Heron Biotechnology Inc
Original Assignee
Blue Heron Biotechnology Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Blue Heron Biotechnology Inc filed Critical Blue Heron Biotechnology Inc
Priority to JP2006535658A priority Critical patent/JP2007508825A/en
Priority to CA002543014A priority patent/CA2543014A1/en
Priority to EP04809957A priority patent/EP1673381A2/en
Publication of WO2005037850A2 publication Critical patent/WO2005037850A2/en
Publication of WO2005037850A3 publication Critical patent/WO2005037850A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Polynucleotides having in excess of 1,000 nucleotides can be prepared using a solid phase synthesis technique. A feature of the technique is the use of a reusable solid support that contains covalently bound oligonucleotide. This covalently bound oligonucleotide is annealed to a bridge oligonucleotide, where the bridge is also annealed to a first oligonucleotide that forms a portion of the target polynucleotide. After the target polynucleotide is synthesized, it can be removed from the solid support under denaturing conditions, and the solid support re-used to prepare additional target polynucleotides. The yield of the target polynucleotide increases when shearing force is applied to the solid support that is linked to the growing oligonucleotide. This shearing force is thought to extend the growing end of the oligonucleotide away from contact with other oligonucleotide bound to the solid support and make that end more accessible to annealing with solution oligonucleotide. The synthesis is conveniently accomplished on a porous frit, where reagents and washing solutions are pumped through the frit.
PCT/US2004/033919 2003-10-15 2004-10-13 Solid phase methods for polynucleotide production Ceased WO2005037850A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2006535658A JP2007508825A (en) 2003-10-15 2004-10-13 Solid phase method for polynucleotide production
CA002543014A CA2543014A1 (en) 2003-10-15 2004-10-13 Solid phase methods for polynucleotide production
EP04809957A EP1673381A2 (en) 2003-10-15 2004-10-13 Solid phase methods for polynucleotide production

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US51177003P 2003-10-15 2003-10-15
US60/511,770 2003-10-15
US51214103P 2003-10-16 2003-10-16
US60/512,141 2003-10-16

Publications (2)

Publication Number Publication Date
WO2005037850A2 WO2005037850A2 (en) 2005-04-28
WO2005037850A3 true WO2005037850A3 (en) 2005-09-09

Family

ID=34468003

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/033919 Ceased WO2005037850A2 (en) 2003-10-15 2004-10-13 Solid phase methods for polynucleotide production

Country Status (4)

Country Link
EP (1) EP1673381A2 (en)
JP (1) JP2007508825A (en)
CA (1) CA2543014A1 (en)
WO (1) WO2005037850A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0385410A2 (en) * 1989-02-28 1990-09-05 Canon Kabushiki Kaisha Partially double-stranded oligonucleotide and method for forming oligonucleotide
US5942609A (en) * 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support
WO2003085094A2 (en) * 2002-04-01 2003-10-16 Blue Heron Biotechnology, Inc. Solid phase methods for polynucleotide production

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6277622B1 (en) * 1997-08-11 2001-08-21 The University Of Sydney Synthetic polynucleotides
US6670127B2 (en) * 1997-09-16 2003-12-30 Egea Biosciences, Inc. Method for assembly of a polynucleotide encoding a target polypeptide

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0385410A2 (en) * 1989-02-28 1990-09-05 Canon Kabushiki Kaisha Partially double-stranded oligonucleotide and method for forming oligonucleotide
US5942609A (en) * 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support
WO2003085094A2 (en) * 2002-04-01 2003-10-16 Blue Heron Biotechnology, Inc. Solid phase methods for polynucleotide production

Also Published As

Publication number Publication date
WO2005037850A2 (en) 2005-04-28
EP1673381A2 (en) 2006-06-28
CA2543014A1 (en) 2005-04-28
JP2007508825A (en) 2007-04-12

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