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WO2005003389A3 - In vitro amplification of dna - Google Patents

In vitro amplification of dna Download PDF

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Publication number
WO2005003389A3
WO2005003389A3 PCT/GB2004/002787 GB2004002787W WO2005003389A3 WO 2005003389 A3 WO2005003389 A3 WO 2005003389A3 GB 2004002787 W GB2004002787 W GB 2004002787W WO 2005003389 A3 WO2005003389 A3 WO 2005003389A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna
unclonable
dna fragment
vitro amplification
amplifying
Prior art date
Application number
PCT/GB2004/002787
Other languages
French (fr)
Other versions
WO2005003389A2 (en
Inventor
George Dickinson
Vanessa Hill
Original Assignee
New Royal Holloway & Bedford
George Dickinson
Vanessa Hill
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New Royal Holloway & Bedford, George Dickinson, Vanessa Hill filed Critical New Royal Holloway & Bedford
Publication of WO2005003389A2 publication Critical patent/WO2005003389A2/en
Publication of WO2005003389A3 publication Critical patent/WO2005003389A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention provides a method of amplifying an unclonable DNA fragment in vitro, comprising circularising the unclonable DNA fragment, adding a DNA polymerase, a dNTP mixture and a primer, and amplifying the unclonable DNA fragment by rolling circle amplification to produce a tandem series of repeats of the unclonable DNA fragment.
PCT/GB2004/002787 2003-06-28 2004-06-25 In vitro amplification of dna WO2005003389A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0315160A GB0315160D0 (en) 2003-06-28 2003-06-28 In vitro amplification of DNA
GB0315160.2 2003-06-28

Publications (2)

Publication Number Publication Date
WO2005003389A2 WO2005003389A2 (en) 2005-01-13
WO2005003389A3 true WO2005003389A3 (en) 2005-03-10

Family

ID=27676273

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2004/002787 WO2005003389A2 (en) 2003-06-28 2004-06-25 In vitro amplification of dna

Country Status (2)

Country Link
GB (1) GB0315160D0 (en)
WO (1) WO2005003389A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1819828A2 (en) * 2004-12-11 2007-08-22 CytoGenix, Inc. Cell free biosynthesis of high-quality nucleic acid and uses thereof
US9139849B2 (en) 2005-04-08 2015-09-22 The United States of America as Represented by the Government of the Department of Health and Human Services Rapid generation of long synthetic centromeric tandem repeats for mammalian artificial chromosome formation
WO2007018744A2 (en) * 2005-08-03 2007-02-15 Cytogenix, Inc. Cell-free biosynthesis of nucleic acid
GB0614825D0 (en) * 2006-07-26 2006-09-06 Medical Res Council Method for amplification of ligation reactions
JP2008289483A (en) 2007-05-25 2008-12-04 Symphogen As Screening of transformant expressable in eukaryote system
US10077459B2 (en) 2016-05-04 2018-09-18 General Electric Company Cell-free protein expression using rolling circle amplification product

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999049079A1 (en) * 1998-03-25 1999-09-30 Ulf Landegren Rolling circle replication of padlock probes
US20030118998A1 (en) * 2001-10-15 2003-06-26 Dean Frank B. Nucleic acid amplification

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999049079A1 (en) * 1998-03-25 1999-09-30 Ulf Landegren Rolling circle replication of padlock probes
US20030118998A1 (en) * 2001-10-15 2003-06-26 Dean Frank B. Nucleic acid amplification

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DEAN F B ET AL: "Rapid amplification of plasmid and phage DNA using Phi29 DNA polymerase and multiply-primed rolling circle amplification", GENOME RESEARCH, COLD SPRING HARBOR LABORATORY PRESS, US, vol. 11, no. 6, June 2001 (2001-06-01), pages 1095 - 1099, XP002223174, ISSN: 1088-9051 *
DETTER JOHN C ET AL: "Isothermal strand-displacement amplification applications for high-throughput genomics.", GENOMICS, vol. 80, no. 6, December 2002 (2002-12-01), pages 691 - 698, XP002310697, ISSN: 0888-7543 *
DHULIPALA R ET AL: "New amplification technologies for DNA preparation.", ABSTRACTS OF THE GENERAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 103, 2003, & 103RD AMERICAN SOCIETY FOR MICROBIOLOGY GENERAL MEETING; WASHINGTON, DC, USA; MAY 18-22, 2003, pages I - 081 URL, XP009041694, ISSN: 1060-2011 *
GUO W X ET AL: "Cloning and expression of full length mouse utrophin: the differential association of utrophin and dystrophin with AChR clusters.", FEBS LETTERS. 2 DEC 1996, vol. 398, no. 2-3, 2 December 1996 (1996-12-02), pages 259 - 264, XP002311207, ISSN: 0014-5793 *
NELSON JOHN R ET AL: "TempliPhi, phi29 DNA polymerase based rolling circle amplification of templates for DNA sequencing.", SUPPLEMENT TO BIOTECHNIQUES, no. June, 2002, pages S44 - S47, XP001204520 *
NELSON JOHN R: "Phi29 DNA polymerase-based methods for genomics applications.", JOURNAL OF CLINICAL LIGAND ASSAY, vol. 25, no. 3, October 2002 (2002-10-01), pages 276 - 279, XP009041683, ISSN: 1081-1672 *

Also Published As

Publication number Publication date
WO2005003389A2 (en) 2005-01-13
GB0315160D0 (en) 2003-08-06

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