[go: up one dir, main page]

WO2004090150A3 - Pcr method using multiple primer pairs and the solution used in it and the use of the method in the detection reagents - Google Patents

Pcr method using multiple primer pairs and the solution used in it and the use of the method in the detection reagents Download PDF

Info

Publication number
WO2004090150A3
WO2004090150A3 PCT/CN2004/000329 CN2004000329W WO2004090150A3 WO 2004090150 A3 WO2004090150 A3 WO 2004090150A3 CN 2004000329 W CN2004000329 W CN 2004000329W WO 2004090150 A3 WO2004090150 A3 WO 2004090150A3
Authority
WO
WIPO (PCT)
Prior art keywords
phase
cycles
primer pairs
multiple primer
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CN2004/000329
Other languages
French (fr)
Chinese (zh)
Other versions
WO2004090150A2 (en
Inventor
Dingbang Xu
Wenhui Xu
Defen Zhu
Wenkai Xie
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CNB031163254A external-priority patent/CN100389206C/en
Priority claimed from CNB031163246A external-priority patent/CN100389205C/en
Application filed by Individual filed Critical Individual
Publication of WO2004090150A2 publication Critical patent/WO2004090150A2/en
Publication of WO2004090150A3 publication Critical patent/WO2004090150A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Communicable Diseases (AREA)
  • AIDS & HIV (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to a method of polymerase chain reaction which use multiple primer pairs and also relates to the solution is used in it. Specifically, the PCR reaction was divided into three phases. During the first phase's 2-25 cycles, annealing temperature varies from 60-80°C. During the third phase's 5-25 cycles; denaturing temperature varies from 65-87°C. The second phase, which consists of 2-4 cycles, exists between the first and the third phase. The annealing temperature of this phase is same with that of the first phase. The method of the invention has overcome the disadvantage in the art that non-specific amplification between the sense and antisence primers and the nonspecific amplification product thus obtained in the multiplex PCR. The method is suitable for the preparation of the rapid detect agents that used in single-tube multiplex PCR and it also has good application future in the detection of the virus, such as HBV, HCV, HIV, HPV and so on, and the variants, stereotype, genotype or the subtype thereof, and in the detection of drug resistant gene of the bacterium such as tubercle bacillus.
PCT/CN2004/000329 2003-04-11 2004-04-09 Pcr method using multiple primer pairs and the solution used in it and the use of the method in the detection reagents Ceased WO2004090150A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CNB031163254A CN100389206C (en) 2003-04-11 2003-04-11 A PCR method with multiple primers, its reaction solution and its application in the preparation of detection reagents
CNB031163246A CN100389205C (en) 2003-04-11 2003-04-11 Nested polymerase chain-type reaction method and its application
CN03116325.4 2003-04-11
CN03116324.6 2003-04-11

Publications (2)

Publication Number Publication Date
WO2004090150A2 WO2004090150A2 (en) 2004-10-21
WO2004090150A3 true WO2004090150A3 (en) 2004-12-29

Family

ID=33160318

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2004/000329 Ceased WO2004090150A2 (en) 2003-04-11 2004-04-09 Pcr method using multiple primer pairs and the solution used in it and the use of the method in the detection reagents

Country Status (1)

Country Link
WO (1) WO2004090150A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103103288B (en) * 2013-01-05 2015-03-11 江苏省农业科学院 Method for rapidly and synchronously detecting wheat yellow mosaic virus and Chinese wheat mosaic virus

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102719564B (en) * 2012-06-25 2013-09-25 广西壮族自治区兽医研究所 Triple polymerase chain reaction (PCR) kit for duck hepatitis virus type I, duck circoviruses and Muscovy duckling parvovirosis and application of triple PCR kit
CN109321642A (en) * 2017-07-31 2019-02-12 广州康昕瑞基因健康科技有限公司 Single tube nested PCR reaction system and amplification method
CN110373485A (en) * 2019-07-30 2019-10-25 中山大学达安基因股份有限公司 A kind of ureaplasma urealyticum, three joint inspection kit of chlamydia trachomatis and gonococcus
CN110592200B (en) * 2019-09-25 2023-04-18 人和未来生物科技（长沙）有限公司 Multiplex PCR method for improving amplification specificity and uniformity
US20230103645A1 (en) 2020-03-16 2023-04-06 The University Of North Carolina At Chapel Hill Compositions and methods for the selective detection of tumor-derived viral dna

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002070751A1 (en) * 2001-03-02 2002-09-12 University Of Pittsburgh Of The Commonwealth System Of Higher Education Pcr method

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002070751A1 (en) * 2001-03-02 2002-09-12 University Of Pittsburgh Of The Commonwealth System Of Higher Education Pcr method

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HENEGARIU O. ET AL.: "Multiplex PCR: critical parametrs and spet-by-step protocol", BIOTECHNIQUES, vol. 23, no. 3, 31 December 1997 (1997-12-31), pages 504 - 511, XP000703350 *
KAPLEY-ATYA ET AL.: "Thermocycling steps and optimization of multiplex PCR", BIOTECHNOLOGY LETTERS, vol. 22, no. 24, 31 December 2000 (2000-12-31), pages 1913 - 1918 *
OLMOS ET AL.: "New device and method for capture, reverse transcription and nested PCR in a single closed-tube", NUCLEIC ACIDS RES., vol. 27, no. 6, 21 December 1999 (1999-12-21), pages 1564 - 1565 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103103288B (en) * 2013-01-05 2015-03-11 江苏省农业科学院 Method for rapidly and synchronously detecting wheat yellow mosaic virus and Chinese wheat mosaic virus

Also Published As

Publication number Publication date
WO2004090150A2 (en) 2004-10-21

Similar Documents

Publication Publication Date Title
Fakruddin et al. Nucleic acid amplification: Alternative methods of polymerase chain reaction
Li et al. Advances in isothermal amplification: novel strategies inspired by biological processes
AU2003272438B2 (en) Helicase dependent amplification of nucleic acids
WO2005089508A3 (en) Dna sequence detection by limited primer extension
WO1999063112A3 (en) Pcr techniques for detecting microbial and viral contaminants in foodstuffs
WO2000056925A3 (en) Methods for single nucleotide polymorphism detection
WO2000066607A8 (en) Detection using degradation of a tagged sequence
AU2121992A (en) Methods and reagents for hepatitis c detection
WO2001071042A3 (en) Detection kits, such as nucleic acid arrays, for detecting the expression of 10,000 or more drosophila genes and uses thereof
WO2002057479A3 (en) Primers and methods for the detection and discrimination of nucleic acids
WO1997018334A3 (en) Nucleic acid probes complementary to human papillomavirus nucleic acid and related methods and kits
WO2002018638A3 (en) Detection of cyp2d6 polymorphisms
WO2008001376A3 (en) Detection of analytes in a medium
EP0776981A3 (en) Oligonucleotide primers for amplifying HCV nucleic acid
WO2004059289A3 (en) Target-dependent transcription using deletion mutants of n4 rna polymerase
WO2004003136A3 (en) Methods of detecting sequence differences
WO2004090150A3 (en) Pcr method using multiple primer pairs and the solution used in it and the use of the method in the detection reagents
JP2004518416A (en) Compositions and methods for detecting human immunodeficiency virus 2 (HIV-2)
WO2009054510A1 (en) Isothermal amplification method and dna polymerase used in the same
CA2392218A1 (en) Simultaneous detection of hbv, hcv and hiv in plasma samples using a multiplex capture assay
BR0311768A (en) Identification of oligonucleotides for the capture, detection and quantification of hepatitis A virus nucleic acid
AU2001287712A1 (en) Method for the specific determination of DNA -sequences by means of parallel amplification
WO2003027258A3 (en) RECOMBINANT DNA PROCESSES USING A dNTP MIXTURE CONTAINING MODIFIED NUCLEOTIDES
WO2006005064A3 (en) 5-methylcytosine detection, compositions and methods therefor
EP2389449A1 (en) Compositions, kits and related methods for the detection and/or monitoring of listeria

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): BW GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DPEN Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed from 20040101)
122 Ep: pct application non-entry in european phase