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WO2004060297A3 - Hydrophilic cross-linking agents for use in enzymatic sensors - Google Patents

Hydrophilic cross-linking agents for use in enzymatic sensors Download PDF

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Publication number
WO2004060297A3
WO2004060297A3 PCT/US2003/041060 US0341060W WO2004060297A3 WO 2004060297 A3 WO2004060297 A3 WO 2004060297A3 US 0341060 W US0341060 W US 0341060W WO 2004060297 A3 WO2004060297 A3 WO 2004060297A3
Authority
WO
WIPO (PCT)
Prior art keywords
sensor
hydrophilic
cross
linking agent
enzymatic
Prior art date
Application number
PCT/US2003/041060
Other languages
French (fr)
Other versions
WO2004060297A2 (en
Inventor
Antwerp William P Van
Original Assignee
Medtronic Minimed Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Medtronic Minimed Inc filed Critical Medtronic Minimed Inc
Priority to AU2003297503A priority Critical patent/AU2003297503A1/en
Priority to CA002511549A priority patent/CA2511549A1/en
Priority to EP03814924A priority patent/EP1587546A4/en
Publication of WO2004060297A2 publication Critical patent/WO2004060297A2/en
Publication of WO2004060297A3 publication Critical patent/WO2004060297A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C12Q1/006—Enzyme electrodes involving specific analytes or enzymes for glucose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/06—Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Emergency Medicine (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

An enzymatic sensor comprising an enzyme that generates a signal upon contact with an analyte; a substrate; and a cross-linking agent that comprises one or more hydrophilic moieties. The enzyme is immobilized in the substrate via the hydrophilic cross-linking agent. A biocompatible membrane, comprising the enzyme, substrate and the hydrophilic cross-linking agent, is applied to a sensor to produce an enzymatic sensor. The enzymatic sensor provides enhanced sensing capabilities by improving the diffusion of reactive species and thereby increasing the overall sensitivity and lifetime of the sensor. The incorporation of hydrophilic moieties into the cross-linking agent optimizes hydration of the sensor and creates hydrophilic channels or pathways for reactive species, such as H202 and the analyte of interest. The membrane and enzymatic sensor can be used in a method for detecting an analyte, such as glucose.
PCT/US2003/041060 2002-12-31 2003-12-19 Hydrophilic cross-linking agents for use in enzymatic sensors WO2004060297A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2003297503A AU2003297503A1 (en) 2002-12-31 2003-12-19 Hydrophilic cross-linking agents for use in enzymatic sensors
CA002511549A CA2511549A1 (en) 2002-12-31 2003-12-19 Hydrophilic cross-linking agents for use in enzymatic sensors
EP03814924A EP1587546A4 (en) 2002-12-31 2003-12-19 Hydrophilic cross-linking agents for use in enzymatic sensors

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33550602A 2002-12-31 2002-12-31
US10/335,506 2002-12-31

Publications (2)

Publication Number Publication Date
WO2004060297A2 WO2004060297A2 (en) 2004-07-22
WO2004060297A3 true WO2004060297A3 (en) 2005-05-06

Family

ID=32710917

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/041060 WO2004060297A2 (en) 2002-12-31 2003-12-19 Hydrophilic cross-linking agents for use in enzymatic sensors

Country Status (4)

Country Link
EP (1) EP1587546A4 (en)
AU (1) AU2003297503A1 (en)
CA (1) CA2511549A1 (en)
WO (1) WO2004060297A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9493805B2 (en) 2001-06-01 2016-11-15 Colorado State University Research Foundation Enzymatic biosensors with enhanced activity retention for detection of organic compounds
US9493806B2 (en) 2001-06-01 2016-11-15 Colorado State University Research Foundation Enzymatic biosensing systems
US9796998B2 (en) 2007-04-09 2017-10-24 Colorado State University Research Foundation Oxygenase-based biosensing systems for measurement of halogenated alkene concentrations
US20110082356A1 (en) * 2009-10-01 2011-04-07 Medtronic Minimed, Inc. Analyte sensor apparatuses having interference rejection membranes and methods for making and using them
WO2012071471A2 (en) 2010-11-22 2012-05-31 Colorado State University Research Foundation Biosensing systems for measurement of lactose
US9499853B2 (en) 2011-08-02 2016-11-22 Colorado State University Research Foundation Biosensing system with extended lifetime via cofactor recycling
US10669153B2 (en) 2017-05-23 2020-06-02 International Business Machines Corporation Neuro-chemical sensor with selectively permeable membrane on nano-electrode

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001022820A1 (en) * 1999-09-30 2001-04-05 The General Hospital Corporation Use of pramipexole as a treatment for cocaine craving
US6485703B1 (en) * 1998-07-31 2002-11-26 The Texas A&M University System Compositions and methods for analyte detection
US20030219404A1 (en) * 1999-12-24 2003-11-27 Motoo Yamasaki Branched polyalkylene glycols

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3106456A1 (en) * 1981-02-21 1982-10-07 Röhm GmbH, 6100 Darmstadt METHOD FOR THE PRODUCTION OF PEARL-SHAPED, HYDROPHILIC, POLYMER-BASED POLYMERS TO PROTEINS
US5786439A (en) * 1996-10-24 1998-07-28 Minimed Inc. Hydrophilic, swellable coatings for biosensors
US5874165A (en) * 1996-06-03 1999-02-23 Gore Enterprise Holdings, Inc. Materials and method for the immobilization of bioactive species onto polymeric subtrates

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6485703B1 (en) * 1998-07-31 2002-11-26 The Texas A&M University System Compositions and methods for analyte detection
WO2001022820A1 (en) * 1999-09-30 2001-04-05 The General Hospital Corporation Use of pramipexole as a treatment for cocaine craving
US20030219404A1 (en) * 1999-12-24 2003-11-27 Motoo Yamasaki Branched polyalkylene glycols

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KAKU T. ET AL: "AMPERIMETRIC GLUCOSE SENSORS BASED ON IMMOBILIZED GLUCOSE OXIDASE-POLYQUINONE SYSTEM", ANAL. CHEM., vol. 66, 1994, pages 1231 - 1235, XP000446067 *
See also references of EP1587546A4 *
YANG Y-Y. ET AL: "POE-PEG-POE triblock copolymeric microspheres containing protein I. Preparation and characterization", JOURNAL OF CONTROLLED RELEASE, vol. 75, 2001, pages 115 - 128, XP004254408 *

Also Published As

Publication number Publication date
WO2004060297A2 (en) 2004-07-22
AU2003297503A8 (en) 2004-07-29
CA2511549A1 (en) 2004-07-22
EP1587546A4 (en) 2007-02-14
AU2003297503A1 (en) 2004-07-29
EP1587546A2 (en) 2005-10-26

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