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WO2004005517A3 - L-amidase from rhizobium huautlense - Google Patents

L-amidase from rhizobium huautlense Download PDF

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Publication number
WO2004005517A3
WO2004005517A3 PCT/EP2003/007148 EP0307148W WO2004005517A3 WO 2004005517 A3 WO2004005517 A3 WO 2004005517A3 EP 0307148 W EP0307148 W EP 0307148W WO 2004005517 A3 WO2004005517 A3 WO 2004005517A3
Authority
WO
WIPO (PCT)
Prior art keywords
amidase
rhizobium huautlense
huautlense
rhizobium
enzyme
Prior art date
Application number
PCT/EP2003/007148
Other languages
French (fr)
Other versions
WO2004005517A2 (en
Inventor
Maria-Regina Kula
Lutz Krieg
Original Assignee
Degussa
Maria-Regina Kula
Lutz Krieg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Degussa, Maria-Regina Kula, Lutz Krieg filed Critical Degussa
Priority to AU2003257434A priority Critical patent/AU2003257434A1/en
Publication of WO2004005517A2 publication Critical patent/WO2004005517A2/en
Publication of WO2004005517A3 publication Critical patent/WO2004005517A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C12P41/006—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by reactions involving C-N bonds, e.g. nitriles, amides, hydantoins, carbamates, lactames, transamination reactions, or keto group formation from racemic mixtures
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/41—Rhizobium

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Catalysts (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention provides a new L-amidase from the organism Rhizobium huautlense. The invention also relates to the nucleic acids which code this enzyme and the vehicles, such as plasmids or microorganisms, containing these. A process for further improvement of these enzymes, their use and a whole cell catalyst containing the enzyme according to the invention are also disclosed.
PCT/EP2003/007148 2002-07-09 2003-07-04 L-amidase from rhizobium huautlense WO2004005517A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003257434A AU2003257434A1 (en) 2002-07-09 2003-07-04 L-amidase from rhizobium huautlense

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE10230756.3 2002-07-09
DE10230756 2002-07-09
DE10318236 2003-04-22
DE10318236.5 2003-04-22

Publications (2)

Publication Number Publication Date
WO2004005517A2 WO2004005517A2 (en) 2004-01-15
WO2004005517A3 true WO2004005517A3 (en) 2004-07-22

Family

ID=30116617

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/007148 WO2004005517A2 (en) 2002-07-09 2003-07-04 L-amidase from rhizobium huautlense

Country Status (2)

Country Link
AU (1) AU2003257434A1 (en)
WO (1) WO2004005517A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102004013842A1 (en) 2004-03-20 2005-10-13 Degussa Ag Nitrile hydratases from metagenome libraries
ITRM20040534A1 (en) 2004-10-29 2005-01-29 Brunangelo Falini MUTANTS OF NUCLEOPHOSMINE PROTEIN (NPM), CORRESPONDING GENETIC SEQUENCES AND RELATED USES.
EP2226382A1 (en) 2009-03-03 2010-09-08 B.R.A.I.N. Biotechnology Research and Information Network AG Protease for wound conditioning and skin care

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4080259A (en) * 1976-01-08 1978-03-21 Novo Industri A/S Process of preparing L and D α-amino acids by enzyme treatment of DL-α-amino acid amide
EP0307023A1 (en) * 1987-08-17 1989-03-15 Novo Nordisk A/S Process for preparation of organic chemicals
US5100782A (en) * 1988-05-06 1992-03-31 Schering Aktiengesellschaft Process for the preparation of l-amino acids and amino acid amides

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4080259A (en) * 1976-01-08 1978-03-21 Novo Industri A/S Process of preparing L and D α-amino acids by enzyme treatment of DL-α-amino acid amide
EP0307023A1 (en) * 1987-08-17 1989-03-15 Novo Nordisk A/S Process for preparation of organic chemicals
US5100782A (en) * 1988-05-06 1992-03-31 Schering Aktiengesellschaft Process for the preparation of l-amino acids and amino acid amides

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1984, KRETOVICH V L ET AL: "LOCALIZATION OF ASPARTASE EC-4.3.1.1 ASPARAGINASE EC-3.5.1.1 AND GLUTAMINASE EC-3.5.1.2 IN INTACT BACTEROIDS OF RHIZOBIUM-LUPINI", XP002267133, Database accession no. PREV198579052908 *
PRIKLADNAYA BIOKHIMIYA I MIKROBIOLOGIYA, vol. 20, no. 4, 1984, pages 445 - 447, ISSN: 0555-1099 *
VAN DEN TWEEL W J J ET AL: "OCHROBACTRUM ANTHROPI NCIMB 40321: A NEW BIOCATALYST WITH BROAD-SPECTRUM L-SPECIFIC AMIDASE ACTIVITY", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER VERLAG, BERLIN, DE, vol. 39, no. 3, 1993, pages 296 - 300, XP008006772, ISSN: 0175-7598 *
WANG E T ET AL: "Rhizobium huautlense sp. nov., a symbiont of Sesbania herbacea that has a close phylogenetic relationship with Rhizobium galegae", INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, vol. 48, no. 3 PART 4, July 1998 (1998-07-01), pages 687 - 699, XP008026470, ISSN: 0020-7713 *

Also Published As

Publication number Publication date
AU2003257434A1 (en) 2004-01-23
WO2004005517A2 (en) 2004-01-15

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