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WO2004005467A3 - Expression of human interferon in transgenic chloroplasts - Google Patents

Expression of human interferon in transgenic chloroplasts Download PDF

Info

Publication number
WO2004005467A3
WO2004005467A3 PCT/US2003/020869 US0320869W WO2004005467A3 WO 2004005467 A3 WO2004005467 A3 WO 2004005467A3 US 0320869 W US0320869 W US 0320869W WO 2004005467 A3 WO2004005467 A3 WO 2004005467A3
Authority
WO
WIPO (PCT)
Prior art keywords
ifn
plastid
expression
plant cell
human interferon
Prior art date
Application number
PCT/US2003/020869
Other languages
French (fr)
Other versions
WO2004005467A2 (en
Inventor
Henry Daniell
Original Assignee
Univ Central Florida
Henry Daniell
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Central Florida, Henry Daniell filed Critical Univ Central Florida
Priority to US10/520,104 priority Critical patent/US20100251425A9/en
Priority to AU2003253781A priority patent/AU2003253781A1/en
Publication of WO2004005467A2 publication Critical patent/WO2004005467A2/en
Publication of WO2004005467A3 publication Critical patent/WO2004005467A3/en
Priority to US14/537,661 priority patent/US9657302B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A plastid transformation vector for a stably transforming a plastid genome is provided. The vector includes, as operably-linked components, a first flanking sequence, a DNA sequence coding for a therapeutic human IFN, which is capable of expression in the plastid and a second flanking sequence. The invention also provides isolated and purified IFN, wherein the IFN is configured in a monomeric or multimeric form and is a structural equivalent to orally administered human IFN. Also provided are methods for variable-expressing biopharmaceutical proteins in plants suitable for mammal consumption. The method includes integrating a plastid transformation vector into a plastid genome of a plant cell; growing the plant cell to express a biopharmaceutical protein, such as therapeutic human interferon IFN. Also disclosed are plants transformed with the aforementioned vectors, and the progeny thereof. Also, disclosed is the IFN, which is IFNα2b.
PCT/US2003/020869 1998-05-15 2003-07-02 Expression of human interferon in transgenic chloroplasts WO2004005467A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US10/520,104 US20100251425A9 (en) 1998-05-15 2003-07-02 Expression of human interferon in transgenic chloroplasts
AU2003253781A AU2003253781A1 (en) 2002-07-03 2003-07-02 Expression of human interferon in transgenic chloroplasts
US14/537,661 US9657302B2 (en) 1998-05-15 2014-11-10 Expression of human interferon in transgenic chloroplasts

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39343802P 2002-07-03 2002-07-03
US60/393,438 2002-07-03

Related Parent Applications (2)

Application Number Title Priority Date Filing Date
US09/807,742 Continuation-In-Part US20030204864A1 (en) 1997-08-07 2001-02-28 Pharmaceutical proteins, human therapeutics, human serum albumin, insulin, native cholera toxic b submitted on transgenic plastids
PCT/US2001/006288 Continuation-In-Part WO2001072959A2 (en) 1998-05-15 2001-02-28 Plastid transformation vectors for expressing human proteins in plants

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10/520,104 A-371-Of-International US20100251425A9 (en) 1998-05-15 2003-07-02 Expression of human interferon in transgenic chloroplasts
US14/537,661 Continuation US9657302B2 (en) 1998-05-15 2014-11-10 Expression of human interferon in transgenic chloroplasts

Publications (2)

Publication Number Publication Date
WO2004005467A2 WO2004005467A2 (en) 2004-01-15
WO2004005467A3 true WO2004005467A3 (en) 2004-04-08

Family

ID=30115578

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/020869 WO2004005467A2 (en) 1998-05-15 2003-07-02 Expression of human interferon in transgenic chloroplasts

Country Status (2)

Country Link
AU (1) AU2003253781A1 (en)
WO (1) WO2004005467A2 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110179530A1 (en) * 2001-01-23 2011-07-21 University Of Central Florida Research Foundation, Inc. Pharmaceutical Proteins, Human Therapeutics, Human Serum Albumin Insulin, Native Cholera Toxin B Subunit on Transgenic Plastids
US20100251425A9 (en) 1998-05-15 2010-09-30 University Of Central Florida Expression of human interferon in transgenic chloroplasts
EP1274846A4 (en) 2000-03-01 2004-09-29 Univ Auburn Plastid transformation vectors for expressing human proteins in plants
GB2426521A (en) 2004-02-02 2006-11-29 Ambrx Inc Modified human four helical bundle polypeptides and their uses
AU2008201682B2 (en) * 2004-02-02 2011-02-24 Ambrx, Inc. Modified human interferon polypeptides and their uses
EP1883701B1 (en) 2005-04-29 2012-01-18 University of Cape Town Expression of viral proteins in plants
US20080274143A1 (en) 2005-05-27 2008-11-06 Henry Daniell Chloroplasts Engineering to Express Pharmaceutical Proteins
WO2008121947A1 (en) 2007-03-30 2008-10-09 University Of Central Florida Research Foundation, Inc. Chloroplasts engineered to express pharmaceutical proteins in edible plants
AU2009212309B2 (en) 2008-02-07 2014-04-03 Ceregene, Inc. Rescue of photoreceptors by intravitreal administration of an expression vector encoding a therapeutic protein
US10689633B2 (en) 2008-02-29 2020-06-23 The Trustees Of The University Of Pennsylvania Expression of β-mannanase in chloroplasts and its utilization in lignocellulosic woody biomass hydrolysis
WO2010033275A2 (en) * 2008-05-27 2010-03-25 University Of Central Florida Research Foundation, Inc. Orally administerable vaccine for yersinia pestis
US9724400B2 (en) 2009-11-09 2017-08-08 The Trustees Of The University Of Pennsylvania Administration of plant expressed oral tolerance agents
US10865419B2 (en) 2011-10-24 2020-12-15 The Trustees Of The University Of Pennsylvania Orally administered plastid expressed cholera toxin B subunit-exendin 4 as treatment for type 2 diabetes
WO2015058214A1 (en) 2013-10-18 2015-04-23 Trustees Of The University Of Pennsylvania Oral delivery of angiotensin converting enzyme 2 (ace2) or angiotensin-(1-7) bioencapsulated in plant cells
AU2014348289B2 (en) 2013-11-15 2020-11-26 Henry Daniell Compositions and methods for suppression of inhibitor formation against factor VIII in Hemophilia A patients
CN108699548A (en) 2015-11-16 2018-10-23 宾夕法尼亚州立大学托管会 Targeted delivery of therapeutic proteins bioencapsulated in plant cells to target cell types for disease treatment

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6512162B2 (en) * 1998-07-10 2003-01-28 Calgene Llc Expression of eukaryotic peptides in plant plastids
US20030204864A1 (en) * 2001-02-28 2003-10-30 Henry Daniell Pharmaceutical proteins, human therapeutics, human serum albumin, insulin, native cholera toxic b submitted on transgenic plastids

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6512162B2 (en) * 1998-07-10 2003-01-28 Calgene Llc Expression of eukaryotic peptides in plant plastids
US20030204864A1 (en) * 2001-02-28 2003-10-30 Henry Daniell Pharmaceutical proteins, human therapeutics, human serum albumin, insulin, native cholera toxic b submitted on transgenic plastids

Also Published As

Publication number Publication date
AU2003253781A1 (en) 2004-01-23
WO2004005467A2 (en) 2004-01-15
AU2003253781A8 (en) 2004-01-23

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