[go: up one dir, main page]

WO2004001103A3 - Silk biomaterials and methods of use thereof - Google Patents

Silk biomaterials and methods of use thereof Download PDF

Info

Publication number
WO2004001103A3
WO2004001103A3 PCT/US2003/019893 US0319893W WO2004001103A3 WO 2004001103 A3 WO2004001103 A3 WO 2004001103A3 US 0319893 W US0319893 W US 0319893W WO 2004001103 A3 WO2004001103 A3 WO 2004001103A3
Authority
WO
WIPO (PCT)
Prior art keywords
biomaterials
silk
processed
methods
avoids
Prior art date
Application number
PCT/US2003/019893
Other languages
French (fr)
Other versions
WO2004001103A2 (en
Inventor
David L Kaplan
Hyoung-Joon Jin
Gregory Rutledge
Sergei Fridrikh
Original Assignee
Univ Tufts
Massachusetts Inst Technology
David L Kaplan
Hyoung-Joon Jin
Gregory Rutledge
Sergei Fridrikh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Tufts, Massachusetts Inst Technology, David L Kaplan, Hyoung-Joon Jin, Gregory Rutledge, Sergei Fridrikh filed Critical Univ Tufts
Priority to AU2003253690A priority Critical patent/AU2003253690A1/en
Publication of WO2004001103A2 publication Critical patent/WO2004001103A2/en
Publication of WO2004001103A3 publication Critical patent/WO2004001103A3/en

Links

Classifications

    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
    • D01D5/00—Formation of filaments, threads, or the like
    • D01D5/0007—Electro-spinning
    • D01D5/0015—Electro-spinning characterised by the initial state of the material
    • D01D5/003—Electro-spinning characterised by the initial state of the material the material being a polymer solution or dispersion
    • D01D5/0038—Electro-spinning characterised by the initial state of the material the material being a polymer solution or dispersion the fibre formed by solvent evaporation, i.e. dry electro-spinning
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
    • C07K14/43586—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from silkworms
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08H—DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS
    • C08H1/00—Macromolecular products derived from proteins
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L89/00—Compositions of proteins; Compositions of derivatives thereof
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
    • D01D1/00—Treatment of filament-forming or like material
    • D01D1/02—Preparation of spinning solutions
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
    • D01F4/00—Monocomponent artificial filaments or the like of proteins; Manufacture thereof
    • D01F4/02—Monocomponent artificial filaments or the like of proteins; Manufacture thereof from fibroin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Textile Engineering (AREA)
  • Insects & Arthropods (AREA)
  • Zoology (AREA)
  • Mechanical Engineering (AREA)
  • Polymers & Plastics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Dispersion Chemistry (AREA)
  • Materials Engineering (AREA)
  • General Chemical & Material Sciences (AREA)
  • Materials For Medical Uses (AREA)

Abstract

The present invention provides an all-aqueaous process for production of silk biomaterials, e.g., fibers, films, foams and mats. In the process, at least one biocompatible polymer, such as poly(ethylene oxide) (EPO)(a well-documented biocompatible material), was blended with the silk protein prior to processing e.g., electrospinning. We discovered that this step avoids problems associated with conformational transitions of fibroin during solubilization and reprocessing from aqueous solution which lead to embrittled materials. Moreover, the processed avoids the use of organic solvents that can pose problems when the processed biomaterials are exposed to cells in vitro or in vivo.
PCT/US2003/019893 2002-06-24 2003-06-24 Silk biomaterials and methods of use thereof WO2004001103A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003253690A AU2003253690A1 (en) 2002-06-24 2003-06-24 Silk biomaterials and methods of use thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39092902P 2002-06-24 2002-06-24
US60/390,929 2002-06-24

Publications (2)

Publication Number Publication Date
WO2004001103A2 WO2004001103A2 (en) 2003-12-31
WO2004001103A3 true WO2004001103A3 (en) 2004-04-08

Family

ID=30000648

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/019893 WO2004001103A2 (en) 2002-06-24 2003-06-24 Silk biomaterials and methods of use thereof

Country Status (2)

Country Link
AU (1) AU2003253690A1 (en)
WO (1) WO2004001103A2 (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8886334B2 (en) 2008-10-07 2014-11-11 Mc10, Inc. Systems, methods, and devices using stretchable or flexible electronics for medical applications
US8934965B2 (en) 2011-06-03 2015-01-13 The Board Of Trustees Of The University Of Illinois Conformable actively multiplexed high-density surface electrode array for brain interfacing
US9012784B2 (en) 2008-10-07 2015-04-21 Mc10, Inc. Extremely stretchable electronics
US9159635B2 (en) 2011-05-27 2015-10-13 Mc10, Inc. Flexible electronic structure
US9171794B2 (en) 2012-10-09 2015-10-27 Mc10, Inc. Embedding thin chips in polymer
US9289132B2 (en) 2008-10-07 2016-03-22 Mc10, Inc. Catheter balloon having stretchable integrated circuitry and sensor array
US9450043B2 (en) 2004-06-04 2016-09-20 The Board Of Trustees Of The University Of Illinois Methods and devices for fabricating and assembling printable semiconductor elements
US9554484B2 (en) 2012-03-30 2017-01-24 The Board Of Trustees Of The University Of Illinois Appendage mountable electronic devices conformable to surfaces

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1613796B1 (en) 2003-04-10 2017-03-22 Tufts University Concentrated aqueous silk fibroin solution and use thereof
EP1773240B1 (en) 2004-06-11 2019-11-20 Trustees of the Tufts College Silk-based drug delivery system
CN100382772C (en) * 2005-09-28 2008-04-23 南通大学 Preparation method of medical nerve graft containing silk fibroin
CN100404081C (en) * 2006-06-09 2008-07-23 浙江大学 A mesh tissue engineering scaffold
JP5896743B2 (en) 2008-10-09 2016-03-30 タフツ ユニバーシティー／トラスティーズ オブ タフツ カレッジ Modified silk film containing glycerol
WO2011041727A1 (en) 2009-10-01 2011-04-07 Mc10, Inc. Protective cases with integrated electronics
US9936574B2 (en) 2009-12-16 2018-04-03 The Board Of Trustees Of The University Of Illinois Waterproof stretchable optoelectronics
US8666471B2 (en) 2010-03-17 2014-03-04 The Board Of Trustees Of The University Of Illinois Implantable biomedical devices on bioresorbable substrates
JP6046491B2 (en) 2009-12-16 2016-12-21 ザ ボード オブ トラスティーズ オブ ザ ユニヴァーシティー オブ イリノイ In vivo electrophysiology using conformal electronics
US10441185B2 (en) 2009-12-16 2019-10-15 The Board Of Trustees Of The University Of Illinois Flexible and stretchable electronic systems for epidermal electronics
US8697057B2 (en) 2010-08-19 2014-04-15 Allergan, Inc. Compositions and soft tissue replacement methods
WO2012031282A2 (en) 2010-09-03 2012-03-08 Tufts University/Trustees Of Tufts College Plasmonic nanoparticle-doped silk materials
US10933173B2 (en) 2010-10-19 2021-03-02 Trustees Of Tufts College Silk fibroin-based microneedles and methods of making the same
EP2678022A2 (en) 2011-02-23 2014-01-01 Allergan, Inc. Compositions and improved soft tissue replacement methods
US9765934B2 (en) 2011-05-16 2017-09-19 The Board Of Trustees Of The University Of Illinois Thermally managed LED arrays assembled by printing
US20140370094A1 (en) 2011-11-08 2014-12-18 Tufts University Silk-based scaffold platform for engineering tissue constructs
MX2014005702A (en) 2011-11-09 2015-02-17 Tufts College Injectable silk fibroin particles and uses thereof.
ES2791769T3 (en) 2011-11-09 2020-11-05 Tufts College Injectable silk fibroin foams and uses thereof
CN108389893A (en) 2011-12-01 2018-08-10 伊利诺伊大学评议会 It is designed to undergo the transient state device of programmable transformation
BR112014016154A8 (en) 2011-12-29 2017-07-04 Tufts College functionalization of biomaterials to control responses to regeneration and inflammation
EP2814527A1 (en) 2012-02-16 2014-12-24 Allergan, Inc. Compositions and improved soft tissue replacement methods
WO2013123272A1 (en) 2012-02-16 2013-08-22 Allergan, Inc. Compositions and improved soft tissue replacement methods
WO2013123270A1 (en) 2012-02-16 2013-08-22 Allergan, Inc. Compositions and improved soft tissue replacement methods
EP2814526B1 (en) 2012-02-16 2016-11-02 Allergan, Inc. Compositions and improved soft tissue replacement methods
WO2013163407A1 (en) 2012-04-25 2013-10-31 Trustees Of Tufts College Silk microspheres and methods for surface lubrication
CN103083224A (en) * 2013-02-06 2013-05-08 东华大学 Method for preparing dexamethasone slow-release medicine carrier
US10677647B2 (en) 2015-06-01 2020-06-09 The Board Of Trustees Of The University Of Illinois Miniaturized electronic systems with wireless power and near-field communication capabilities
EP3304130B1 (en) 2015-06-01 2021-10-06 The Board of Trustees of the University of Illinois Alternative approach to uv sensing
EP3766686A1 (en) 2015-09-17 2021-01-20 Roberto Velozzi Jerez Load-bearing composite panels, materials, products, and processes to make and use same
US10925543B2 (en) 2015-11-11 2021-02-23 The Board Of Trustees Of The University Of Illinois Bioresorbable silicon electronics for transient implants
US11617815B2 (en) 2016-10-31 2023-04-04 Sofregen Medical, Inc. Compositions comprising silk fibroin particles and uses thereof
EP3773477A1 (en) 2018-04-03 2021-02-17 Vaxess Technologies, Inc. Microneedle comprising silk fibroin applied to a dissolvable base
WO2021076798A1 (en) 2019-10-15 2021-04-22 Sofregen Medical, Inc. Delivery devices for delivering and methods of delivering compositions
WO2023250117A2 (en) 2022-06-24 2023-12-28 Vaxess Technologies, Inc. Applicator for medicament patch
WO2024263835A1 (en) 2023-06-23 2024-12-26 Vaxess Technologies, Inc. Applicator for medicament patch
WO2025004113A1 (en) * 2023-06-29 2025-01-02 Serigen Mediproducts Pvt. Ltd A non-adherent absorbent article

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60259677A (en) * 1984-05-31 1985-12-21 水島　繁三郎 Raw yarn, fabric and knitted fabric comprising animal protein adsorbed regeneration fiber and its production
US4820418A (en) * 1987-02-03 1989-04-11 Agency Of Industrial Science & Technology Water-alcohol separating membrane and method for separation of water and alcohol by the use thereof
US5728810A (en) * 1990-04-20 1998-03-17 University Of Wyoming Spider silk protein
US6110590A (en) * 1998-04-15 2000-08-29 The University Of Akron Synthetically spun silk nanofibers and a process for making the same
US6175053B1 (en) * 1997-06-18 2001-01-16 Japan As Represented By Director General Of National Institute Of Sericultural And Entomological Science Ministry Of Agriculture, Forrestry And Fisheries Wound dressing material containing silk fibroin and sericin as main component and method for preparing same

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60259677A (en) * 1984-05-31 1985-12-21 水島　繁三郎 Raw yarn, fabric and knitted fabric comprising animal protein adsorbed regeneration fiber and its production
US4820418A (en) * 1987-02-03 1989-04-11 Agency Of Industrial Science & Technology Water-alcohol separating membrane and method for separation of water and alcohol by the use thereof
US5728810A (en) * 1990-04-20 1998-03-17 University Of Wyoming Spider silk protein
US6175053B1 (en) * 1997-06-18 2001-01-16 Japan As Represented By Director General Of National Institute Of Sericultural And Entomological Science Ministry Of Agriculture, Forrestry And Fisheries Wound dressing material containing silk fibroin and sericin as main component and method for preparing same
US6110590A (en) * 1998-04-15 2000-08-29 The University Of Akron Synthetically spun silk nanofibers and a process for making the same

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
CHEN ET AL.: "pH sensitivity and ion sensitivity of hydrogels based on complex-forming chitosan/silk fibroin interpenetrating polymer network", J. APPL. POLYMER SCI., vol. 65, 1997, pages 2257 - 2262, XP002970823 *
CHEN ET AL.: "Separation of alcohol-water mixture by pervaporation through a novel natural polymer blend membrane-chitosan/silk fibroin blend membrane", J. APPL. POLYMER SCI., vol. 73, 1999, pages 975 - 980, XP002973764 *
FREDDI ET AL.: "Silk fibroin/cellulose blend films: Preparation, structure and physical properties", J. APPL. POLYMER SCI., vol. 56, 1995, pages 1537 - 1545, XP002973763 *
HUANG ET AL.: "Generation of synthetic elastin-mimetic small diameter fibers and fiber networks", MACROMOLECULES, vol. 33, 2000, pages 2989 - 2997, XP002970824 *
JIN ET AL.: "Electrospinning Bombyx mori silk with poly(ethylene oxide)", BIOMACROMOLECULES, vol. 3, 2002, pages 1233 - 1239, XP002970825 *
KWEON ET AL.: "Preparation of semi-interpenetrating polymer networks composed of silk fibroin and poly(ethylene glycol) macromer", J. APPL. POLYMER SCI., vol. 80, 2001, pages 1848 - 1853, XP002973765 *
LIANG ET AL.: "Improvements of the physical properties of fibroin membranes with sodium alginate", J. APPL. POLYMER SCI., vol. 45, 1992, pages 1937 - 1943, XP002970822 *

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9450043B2 (en) 2004-06-04 2016-09-20 The Board Of Trustees Of The University Of Illinois Methods and devices for fabricating and assembling printable semiconductor elements
US8886334B2 (en) 2008-10-07 2014-11-11 Mc10, Inc. Systems, methods, and devices using stretchable or flexible electronics for medical applications
US9012784B2 (en) 2008-10-07 2015-04-21 Mc10, Inc. Extremely stretchable electronics
US9289132B2 (en) 2008-10-07 2016-03-22 Mc10, Inc. Catheter balloon having stretchable integrated circuitry and sensor array
US9159635B2 (en) 2011-05-27 2015-10-13 Mc10, Inc. Flexible electronic structure
US8934965B2 (en) 2011-06-03 2015-01-13 The Board Of Trustees Of The University Of Illinois Conformable actively multiplexed high-density surface electrode array for brain interfacing
US9554484B2 (en) 2012-03-30 2017-01-24 The Board Of Trustees Of The University Of Illinois Appendage mountable electronic devices conformable to surfaces
US9171794B2 (en) 2012-10-09 2015-10-27 Mc10, Inc. Embedding thin chips in polymer

Also Published As

Publication number Publication date
AU2003253690A1 (en) 2004-01-06
WO2004001103A2 (en) 2003-12-31
AU2003253690A8 (en) 2004-01-06

Similar Documents

Publication Publication Date Title
WO2004001103A3 (en) Silk biomaterials and methods of use thereof
WO2004000915A3 (en) Silk biomaterials and methods of use thereof
Dadras Chomachayi et al. Electrospun nanofibers comprising of silk fibroin/gelatin for drug delivery applications: Thyme essential oil and doxycycline monohydrate release study
Li et al. Preparation and properties of cellulose nanocrystals reinforced collagen composite films
Sayed et al. Enhancing mechanical and biodegradation properties of polyvinyl alcohol/silk fibroin nanofibers composite patches for Cardiac Tissue Engineering
Qiu et al. Complete recombinant silk-elastinlike protein-based tissue scaffold
CA2440143A1 (en) Silk-based fibre
Dippold et al. Novel electrospun poly (glycerol sebacate)–zein fiber mats as candidate materials for cardiac tissue engineering
Prado-Prone et al. Antibacterial composite membranes of polycaprolactone/gelatin loaded with zinc oxide nanoparticles for guided tissue regeneration
Kai et al. Engineering highly stretchable lignin-based electrospun nanofibers for potential biomedical applications
Yoon et al. Fabrication of Microfibrous and Nano‐/Microfibrous Scaffolds: Melt and Hybrid Electrospinning and Surface Modification of Poly (L‐lactic acid) with Plasticizer
Zulkifli et al. In vitro degradation study of novel HEC/PVA/collagen nanofibrous scaffold for skin tissue engineering applications
Bet et al. Characterization of polyanionic collagen prepared by selective hydrolysis of asparagine and glutamine carboxyamide side chains
EP3414373B1 (en) Composite materials comprising synthetic dragline spider silk
Hardy et al. Engineered Spider Silk Protein‐B ased Composites for Drug Delivery
WO2007126411A3 (en) Biocompatible polymers and methods of use
Bae et al. Electrospinning and dual crosslinking of water-soluble silk fibroin modified with glycidyl methacrylate
Salvatore et al. Potential of electrospun poly (3‐hydroxybutyrate)/collagen blends for tissue engineering applications
Källrot et al. Covalent grafting of poly (L-lactide) to tune the in vitro degradation rate
ATE525438T1 (en) FLAME-RESISTANT FLEXIBLE THERMOPLASTIC COMPOSITION, METHOD OF PRODUCTION AND ARTICLES THEREOF
MY160836A (en) Modified resin systems for liquid resin infusion applications & process methods related thereto
Mi et al. Properties and fibroblast cellular response of soft and hard thermoplastic polyurethane electrospun nanofibrous scaffolds
Li et al. Fabrication of photo-crosslinkable poly (trimethylene carbonate)/polycaprolactone nanofibrous scaffolds for tendon regeneration
CN116966345A (en) 3D printable biogel and method of use thereof
CN108084676A (en) A kind of low-temp thermoplastic material

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP