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WO2003048732A3 - Multiplexed methylation detection methods - Google Patents

Multiplexed methylation detection methods Download PDF

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Publication number
WO2003048732A3
WO2003048732A3 PCT/US2002/038672 US0238672W WO03048732A3 WO 2003048732 A3 WO2003048732 A3 WO 2003048732A3 US 0238672 W US0238672 W US 0238672W WO 03048732 A3 WO03048732 A3 WO 03048732A3
Authority
WO
WIPO (PCT)
Prior art keywords
detection methods
methylation detection
multiplexed
multiplexed methylation
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2002/038672
Other languages
French (fr)
Other versions
WO2003048732A2 (en
Inventor
Jian-Bing Fan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Inc
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/779,376 external-priority patent/US20020006617A1/en
Application filed by Illumina Inc filed Critical Illumina Inc
Priority to EP02795731A priority Critical patent/EP1461458A4/en
Priority to CA002467814A priority patent/CA2467814A1/en
Priority to AU2002360474A priority patent/AU2002360474A1/en
Publication of WO2003048732A2 publication Critical patent/WO2003048732A2/en
Publication of WO2003048732A3 publication Critical patent/WO2003048732A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention is directed to sensitive and accurate multiplexed assays for target analyte detection and detection of methylation in nucleic acid samples.
PCT/US2002/038672 2001-02-07 2002-12-03 Multiplexed methylation detection methods Ceased WO2003048732A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP02795731A EP1461458A4 (en) 2001-12-03 2002-12-03 Multiplexed methylation detection methods
CA002467814A CA2467814A1 (en) 2001-12-03 2002-12-03 Multiplexed methylation detection methods
AU2002360474A AU2002360474A1 (en) 2001-12-03 2002-12-03 Multiplexed methylation detection methods

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/779,376 US20020006617A1 (en) 2000-02-07 2001-02-07 Nucleic acid detection methods using universal priming
US33695801P 2001-12-03 2001-12-03
US60/336,958 2001-12-03

Publications (2)

Publication Number Publication Date
WO2003048732A2 WO2003048732A2 (en) 2003-06-12
WO2003048732A3 true WO2003048732A3 (en) 2003-09-25

Family

ID=32823667

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/038672 Ceased WO2003048732A2 (en) 2001-02-07 2002-12-03 Multiplexed methylation detection methods

Country Status (4)

Country Link
EP (1) EP1461458A4 (en)
AU (1) AU2002360474A1 (en)
CA (1) CA2467814A1 (en)
WO (1) WO2003048732A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US8906626B2 (en) 2000-02-07 2014-12-09 Illumina, Inc. Multiplex nucleic acid reactions

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
EP1860200A1 (en) * 2001-12-03 2007-11-28 Illumina, Inc. Multiplexed methylation detection methods
US8150627B2 (en) 2003-05-15 2012-04-03 Illumina, Inc. Methods and compositions for diagnosing lung cancer with specific DNA methylation patterns
US8150626B2 (en) 2003-05-15 2012-04-03 Illumina, Inc. Methods and compositions for diagnosing lung cancer with specific DNA methylation patterns
US7799525B2 (en) 2003-06-17 2010-09-21 Human Genetic Signatures Pty Ltd. Methods for genome amplification
CA2537810C (en) 2003-09-04 2012-12-18 Human Genetic Signatures Pty Ltd Nucleic acid detection assay
US8168777B2 (en) 2004-04-29 2012-05-01 Human Genetic Signatures Pty. Ltd. Bisulphite reagent treatment of nucleic acid
EP1794173B1 (en) 2004-09-10 2010-08-04 Human Genetic Signatures PTY Ltd Amplification blocker comprising intercalating nucleic acids (ina) containing intercalating pseudonucleotides (ipn)
WO2006058393A1 (en) 2004-12-03 2006-06-08 Human Genetic Signatures Pty Ltd Methods for simplifying microbial nucleic acids by chemical modification of cytosines
WO2006125267A1 (en) 2005-05-26 2006-11-30 Human Genetic Signatures Pty Ltd Isothermal strand displacement amplification using primers containing a non-regular base
US7901882B2 (en) 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
US9017971B2 (en) 2008-11-05 2015-04-28 Stichting Sanquin Bloedvoorziening Means and methods for investigating nucleic acid sequences
ES2613743T3 (en) 2011-09-07 2017-05-25 Human Genetic Signatures Pty Ltd Molecular detection assay
CN102559661B (en) * 2012-01-18 2014-06-04 厦门基科生物科技有限公司 Novel amplification method and application of ligase reaction mediate

Family Cites Families (14)

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Publication number Priority date Publication date Assignee Title
EP0139489A3 (en) * 1983-09-26 1988-01-13 Ortho Diagnostic Systems Inc. Sandwich hybridization method for nucleic acid detection
EP0238332A2 (en) * 1986-03-19 1987-09-23 Cetus Corporation Liquid hybridization method and kit for detecting the presence of nucleic acid sequences in samples
US5232829A (en) * 1989-09-29 1993-08-03 Hoffmann-La Roche Inc. Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes
US5387505A (en) * 1990-05-04 1995-02-07 Eastman Kodak Company Preparation and isolation of single-stranded biotinylated nucleic acids by heat avidin-biotin cleavage
DE69327326T2 (en) * 1992-07-24 2001-08-16 Diatech Pty. Ltd., Brisbane REPLACEMENT AND DETECTION PROCESS
AU5495194A (en) * 1993-02-26 1994-09-01 Becton Dickinson & Company Flow through membrane nucleic acid hybridization assay
EP0812211A4 (en) * 1994-03-18 1998-12-16 Gen Hospital Corp DETECTION METHOD BY RFLP
US5942391A (en) * 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
US5786146A (en) * 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
AU7829398A (en) * 1997-06-09 1998-12-30 University Of Southern California A cancer diagnostic method based upon dna methylation differences
AU8267998A (en) * 1997-06-24 1999-01-04 Trustees Of Boston University High density streptavidin supports
WO1999001580A1 (en) * 1997-07-03 1999-01-14 Case Western Reserve University Compositions and methods for detection of genomic imprinting disorders
US7700324B1 (en) * 1998-11-03 2010-04-20 The Johns Hopkins University School Of Medicine Methylated CpG island amplification (MCA)
US6331393B1 (en) * 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JONES ET AL.: "Fluorescent and radioactive solid phase dideoxy sequencing of pcr products in microtitre plates", DNA SEQUENCE- J. DNA SEQUENCE AND MAPPING, vol. 1, 1991, pages 279 - 283, XP002967422 *
ROTH ET AL.: "Biotin-avidin microplate assay for the quantitative analysis of enzymatic methylation of DNA by DNA methyltransferases", BIOL. CHEM., vol. 381, March 2000 (2000-03-01), pages 269 - 272, XP002967421 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US8906626B2 (en) 2000-02-07 2014-12-09 Illumina, Inc. Multiplex nucleic acid reactions

Also Published As

Publication number Publication date
EP1461458A2 (en) 2004-09-29
CA2467814A1 (en) 2003-06-12
AU2002360474A1 (en) 2003-06-17
WO2003048732A2 (en) 2003-06-12
EP1461458A4 (en) 2005-10-26

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