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WO2003012106A3 - Regulating nucleic acid for expressing a polynucleotide of interest specifically in the endothelium of a plant seed and uses thereof - Google Patents

Regulating nucleic acid for expressing a polynucleotide of interest specifically in the endothelium of a plant seed and uses thereof Download PDF

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Publication number
WO2003012106A3
WO2003012106A3 PCT/FR2002/002784 FR0202784W WO03012106A3 WO 2003012106 A3 WO2003012106 A3 WO 2003012106A3 FR 0202784 W FR0202784 W FR 0202784W WO 03012106 A3 WO03012106 A3 WO 03012106A3
Authority
WO
WIPO (PCT)
Prior art keywords
polynucleotide
nucleic acid
endothelium
expressing
plant seed
Prior art date
Application number
PCT/FR2002/002784
Other languages
French (fr)
Other versions
WO2003012106A2 (en
Inventor
Loic Lepiniec
Isabelle Debeaujon
Martine Devic
Michel Caboche
Original Assignee
Agronomique Inst Nat Rech
Centre Nat Rech Scient
Loic Lepiniec
Isabelle Debeaujon
Martine Devic
Michel Caboche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agronomique Inst Nat Rech, Centre Nat Rech Scient, Loic Lepiniec, Isabelle Debeaujon, Martine Devic, Michel Caboche filed Critical Agronomique Inst Nat Rech
Priority to EP02772481A priority Critical patent/EP1414966A2/en
Publication of WO2003012106A2 publication Critical patent/WO2003012106A2/en
Publication of WO2003012106A3 publication Critical patent/WO2003012106A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/829—Female sterility

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention concerns a nucleic acid comprising regulating signals for expressing a polynucleotide of interest, specifically in the endothelium and/or the albumen of a plant seed, when said polynucleotide of interest is placed under the control of said regulating signals, said nucleic acid having at least 80 % identity in nucleotides with the sequence SEQ ID No. 1, or with a fragment of at least 200 consecutive nucleotides of the sequence SEQ ID No. 1.
PCT/FR2002/002784 2001-08-01 2002-08-01 Regulating nucleic acid for expressing a polynucleotide of interest specifically in the endothelium of a plant seed and uses thereof WO2003012106A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP02772481A EP1414966A2 (en) 2001-08-01 2002-08-01 Regulating nucleic acid for expressing a polynucleotide of interest specifically in the endothelium of a plant seed and uses thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0110365A FR2828210B1 (en) 2001-08-01 2001-08-01 REGULATING NUCLEIC ACID FOR THE EXPRESSION OF A POLYNUCLEOTIDE OF INTEREST SPECIFICALLY IN THE ENDOTHELIUM OF A PLANT SEED, AND ITS APPLICATIONS
FR0110365 2001-08-01

Publications (2)

Publication Number Publication Date
WO2003012106A2 WO2003012106A2 (en) 2003-02-13
WO2003012106A3 true WO2003012106A3 (en) 2003-11-06

Family

ID=8866217

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2002/002784 WO2003012106A2 (en) 2001-08-01 2002-08-01 Regulating nucleic acid for expressing a polynucleotide of interest specifically in the endothelium of a plant seed and uses thereof

Country Status (3)

Country Link
EP (1) EP1414966A2 (en)
FR (1) FR2828210B1 (en)
WO (1) WO2003012106A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004087878A2 (en) * 2003-03-28 2004-10-14 Monsanto Technology, Llc Novel plant promoters for use in early seed development
US7709701B2 (en) 2004-07-09 2010-05-04 The Samuel Roberts Noble Foundation Genetic manipulation of condensed tannins

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1033405A2 (en) * 1999-02-25 2000-09-06 Ceres Incorporated Sequence-determined DNA fragments and corresponding polypeptides encoded thereby
FR2794772A1 (en) * 1999-06-10 2000-12-15 Agronomique Inst Nat Rech PROMOTER ALLOWING THE EXPRESSION OF TRANSGENES INTO THE PLANT HORIZED IN THE SEED
FR2799203A1 (en) * 1999-10-01 2001-04-06 Biogemma Fr New plant promoters providing albumen-specific expression, useful for preparing transgenic plants with altered starch and oil contents

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1033405A2 (en) * 1999-02-25 2000-09-06 Ceres Incorporated Sequence-determined DNA fragments and corresponding polypeptides encoded thereby
FR2794772A1 (en) * 1999-06-10 2000-12-15 Agronomique Inst Nat Rech PROMOTER ALLOWING THE EXPRESSION OF TRANSGENES INTO THE PLANT HORIZED IN THE SEED
FR2799203A1 (en) * 1999-10-01 2001-04-06 Biogemma Fr New plant promoters providing albumen-specific expression, useful for preparing transgenic plants with altered starch and oil contents

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
COLOMBO L ET AL: "Downregulation of ovule-specific MADS box genes from petunia results in maternally controlled defects in seed development", PLANT CELL, AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MD, US, vol. 9, no. 5, 1 May 1997 (1997-05-01), pages 703 - 715, XP002082109, ISSN: 1040-4651 *
DATABASE EM_GSS [online] EMBL; 8 October 1998 (1998-10-08), FENG ET AL.: "GSS", XP002198219, retrieved from EBI accession no. AQ250609 Database accession no. AQ250609 *
DATABASE EM_PL [online] EMBL; 30 October 1998 (1998-10-30), FEDERSPIEL ET AL.: "Arabidopsis thaliana chromosome I BAC T13M11 genomic sequence, complete sequence", XP002198221, retrieved from EBI accession no. AC005882 Database accession no. AC005882 *
DATABASE GSN [online] EMBL; 18 October 2000 (2000-10-18), ALEXANDROV ET AL.: "Arabidopsis thaliana DNA fragment SEQ ID NO: 61954", XP002198220, retrieved from EBI accession no. AAC49655 Database accession no. AAC49655 *
FOBERT P R ET AL: "T-DNA TAGGING OF A SEED COAT-SPECIFIC CRYPTIC PROMOTER IN TOBACCO", PLANT JOURNAL, BLACKWELL SCIENTIFIC PUBLICATIONS, OXFORD, GB, vol. 6, no. 4, 1994, pages 567 - 577, XP002030412, ISSN: 0960-7412 *

Also Published As

Publication number Publication date
EP1414966A2 (en) 2004-05-06
FR2828210B1 (en) 2004-08-06
WO2003012106A2 (en) 2003-02-13
FR2828210A1 (en) 2003-02-07

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