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WO2002077275A3 - Diagnostic method for determining abnormality of cloned embryos - Google Patents

Diagnostic method for determining abnormality of cloned embryos Download PDF

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Publication number
WO2002077275A3
WO2002077275A3 PCT/GB2002/001398 GB0201398W WO02077275A3 WO 2002077275 A3 WO2002077275 A3 WO 2002077275A3 GB 0201398 W GB0201398 W GB 0201398W WO 02077275 A3 WO02077275 A3 WO 02077275A3
Authority
WO
WIPO (PCT)
Prior art keywords
embryo
cloned
determining
embryos
diagnostic method
Prior art date
Application number
PCT/GB2002/001398
Other languages
French (fr)
Other versions
WO2002077275A2 (en
Inventor
Wendy Lee Dean
Wolf Reik
Original Assignee
Babraham Inst
Wendy Lee Dean
Wolf Reik
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Babraham Inst, Wendy Lee Dean, Wolf Reik filed Critical Babraham Inst
Priority to AU2002244843A priority Critical patent/AU2002244843A1/en
Publication of WO2002077275A2 publication Critical patent/WO2002077275A2/en
Publication of WO2002077275A3 publication Critical patent/WO2002077275A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention relates to a method for determining abnormal development of cloned embryos. The method provides for the detection and/or measurement of aberrant epigenetic reprogramming in cloned mammalian embryos. Aberrant reprogramming may lead to abnormality in cloned embryos. According to a first aspect of the invention there is provided a method for detecting and/or measuring aberrant epigenetic reprogramming in a cloned mammalian embryo, which includes the step of determining the methylation state of said embryo. According to a second aspect of the invention there is provided a method of diagnosis of aberrant epigenetic reprogramming in a cloned mammalian embryo, which includes the step of determining the methylation state of said embryo. Furthermore, the invention provides a method of diagnosis of the propensity of a cloned mammalian embryo for abnormal development, which method includes the step of determining the methylation state of said embryo.
PCT/GB2002/001398 2001-03-23 2002-03-22 Diagnostic method for determining abnormality of cloned embryos WO2002077275A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002244843A AU2002244843A1 (en) 2001-03-23 2002-03-22 Diagnostic method for determining abnormality of cloned embryos

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0107267.7 2001-03-23
GB0107267A GB0107267D0 (en) 2001-03-23 2001-03-23 Diagnostic method for determining the methylation state of cloned embryos

Publications (2)

Publication Number Publication Date
WO2002077275A2 WO2002077275A2 (en) 2002-10-03
WO2002077275A3 true WO2002077275A3 (en) 2003-08-07

Family

ID=9911372

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/001398 WO2002077275A2 (en) 2001-03-23 2002-03-22 Diagnostic method for determining abnormality of cloned embryos

Country Status (3)

Country Link
AU (1) AU2002244843A1 (en)
GB (1) GB0107267D0 (en)
WO (1) WO2002077275A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1428889A1 (en) * 2002-12-10 2004-06-16 Epigenomics AG Method for monitoring the transition of a cell from one state into another
MX2008001709A (en) 2005-08-01 2008-11-26 Nupotential Inc Production of reprogrammed cells with restored potential.
CN113403258B (en) * 2021-05-19 2023-03-14 南方医科大学 Application of histone H3K79 methyltransferase inhibitor in improving animal round sperm injection embryo development efficiency

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998029108A2 (en) * 1996-12-30 1998-07-09 The Johns Hopkins University School Of Medicine Compositions and methods for restoring a normal pattern of imprinting to cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998029108A2 (en) * 1996-12-30 1998-07-09 The Johns Hopkins University School Of Medicine Compositions and methods for restoring a normal pattern of imprinting to cells

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
KIKYO NOBUAKI ET AL: "Reprogramming nuclei: Insights from cloning, nuclear transfer and heterokaryons.", JOURNAL OF CELL SCIENCE, vol. 113, no. 1, January 2000 (2000-01-01), pages 11 - 20, XP002237278, ISSN: 0021-9533 *
KONO T: "Nuclear transfer and reprogramming.", REVIEWS OF REPRODUCTION. ENGLAND MAY 1997, vol. 2, no. 2, May 1997 (1997-05-01), pages 74 - 80, XP002237279, ISSN: 1359-6004 *
REA STEPHEN ET AL: "Regulation of chromatin structure by site-specific histone H3 methyltransferases", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 406, no. 6796, August 2000 (2000-08-01), pages 593 - 599, XP002154907, ISSN: 0028-0836 *
ROUGIER NATHALIE ET AL: "Chromosome methylation patterns during mammalian preimplantation development.", GENES & DEVELOPMENT, vol. 12, no. 14, 15 July 1998 (1998-07-15), pages 2108 - 2113, XP002237281, ISSN: 0890-9369 *
WARNECKE PETER M ET AL: "Bisulfite sequencing in preimplantation embryos: DNA methylation profile of the upstream region of the mouse imprinted H19 gene.", GENOMICS, vol. 51, no. 2, 15 July 1998 (1998-07-15), pages 182 - 190, XP002237280, ISSN: 0888-7543 *

Also Published As

Publication number Publication date
GB0107267D0 (en) 2001-05-16
AU2002244843A1 (en) 2002-10-08
WO2002077275A2 (en) 2002-10-03

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