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WO2002042749A3 - Method and apparatus for measuring apoptosis and growth kinetics - Google Patents

Method and apparatus for measuring apoptosis and growth kinetics Download PDF

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Publication number
WO2002042749A3
WO2002042749A3 PCT/US2001/046195 US0146195W WO0242749A3 WO 2002042749 A3 WO2002042749 A3 WO 2002042749A3 US 0146195 W US0146195 W US 0146195W WO 0242749 A3 WO0242749 A3 WO 0242749A3
Authority
WO
WIPO (PCT)
Prior art keywords
optical density
apoptosis
time
cell population
observance
Prior art date
Application number
PCT/US2001/046195
Other languages
French (fr)
Other versions
WO2002042749A9 (en
WO2002042749A2 (en
Inventor
Vladimir D Kravtsov
Original Assignee
Univ Vanderbilt
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Vanderbilt filed Critical Univ Vanderbilt
Priority to AU2002225874A priority Critical patent/AU2002225874A1/en
Publication of WO2002042749A2 publication Critical patent/WO2002042749A2/en
Publication of WO2002042749A3 publication Critical patent/WO2002042749A3/en
Publication of WO2002042749A9 publication Critical patent/WO2002042749A9/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/251—Colorimeters; Construction thereof
    • G01N21/253—Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus

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  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

A computer-based method of performing optical density measurements on a plurality of cell population samples.In the preferred embodiment of the invention, a calibration factor is first established to eliminate the effect of the media used in the wells. Optical density measurements are then made every five minutes on the cell population samples in each of the wells. For measurement of apoptosis, every five minutes it is also necessary to compute for each cell population the slope of the optical density measurement versus time and correct for any effects attributable to the cell populations in the control sample. The values of interest include maximum corrected slope value, the time from initiation of the assay to observance of the maximum optical density value, the time from initiation of the assay to the beginning of apoptosis and the time from the beginning of apoptosis to observance of maximum optical density value.
PCT/US2001/046195 2000-11-21 2001-11-08 Method and apparatus for measuring apoptosis and growth kinetics WO2002042749A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002225874A AU2002225874A1 (en) 2000-11-21 2001-11-08 Method and apparatus for measuring apoptosis and growth kinetics

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US72134300A 2000-11-21 2000-11-21
US09/721,343 2000-11-21

Publications (3)

Publication Number Publication Date
WO2002042749A2 WO2002042749A2 (en) 2002-05-30
WO2002042749A3 true WO2002042749A3 (en) 2002-11-21
WO2002042749A9 WO2002042749A9 (en) 2003-04-17

Family

ID=24897588

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/046195 WO2002042749A2 (en) 2000-11-21 2001-11-08 Method and apparatus for measuring apoptosis and growth kinetics

Country Status (2)

Country Link
AU (1) AU2002225874A1 (en)
WO (1) WO2002042749A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112012024619A2 (en) * 2010-03-31 2016-05-31 Diatech Oncology Llc system and method for evaluating anticancer drug candidate

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6077684A (en) * 1996-11-14 2000-06-20 Vanderbilt University Automated assay for measuring apoptosis in cell culture

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6077684A (en) * 1996-11-14 2000-06-20 Vanderbilt University Automated assay for measuring apoptosis in cell culture

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KRAVTSOV V D ET AL: "Automated monitoring of apoptosis in suspension cell cultures.", LABORATORY INVESTIGATION;A JOURNAL OF TECHNICAL METHODS AND PATHOLOGY. UNITED STATES FEB 1996, vol. 74, no. 2, February 1996 (1996-02-01), pages 557 - 570, XP002207509, ISSN: 0023-6837 *
MATT SCANLAN: "Chemotherapy Testing Device", INTERNET ARTICLE, 22 October 1997 (1997-10-22), XP002207510, Retrieved from the Internet <URL:http://www.eurekalert.org/pub_releases/1997-10/VUMC-CTD-221097.php> [retrieved on 20020723] *

Also Published As

Publication number Publication date
AU2002225874A1 (en) 2002-06-03
WO2002042749A9 (en) 2003-04-17
WO2002042749A2 (en) 2002-05-30

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