[go: up one dir, main page]

WO2002018952A3 - Biosensor assay device and method - Google Patents

Biosensor assay device and method Download PDF

Info

Publication number
WO2002018952A3
WO2002018952A3 PCT/CA2001/001246 CA0101246W WO0218952A3 WO 2002018952 A3 WO2002018952 A3 WO 2002018952A3 CA 0101246 W CA0101246 W CA 0101246W WO 0218952 A3 WO0218952 A3 WO 0218952A3
Authority
WO
WIPO (PCT)
Prior art keywords
coil
analyte
sample
peptide
biosensor
Prior art date
Application number
PCT/CA2001/001246
Other languages
French (fr)
Other versions
WO2002018952A2 (en
Inventor
Wah Y Wong
Heman Chao
Jerry Mcelroy
Donald Segal
Original Assignee
Helix Biopharma Corp
Wah Y Wong
Heman Chao
Jerry Mcelroy
Donald Segal
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Helix Biopharma Corp, Wah Y Wong, Heman Chao, Jerry Mcelroy, Donald Segal filed Critical Helix Biopharma Corp
Priority to EP01966907A priority Critical patent/EP1314036A2/en
Priority to AU2001287451A priority patent/AU2001287451A1/en
Priority to CA002420743A priority patent/CA2420743A1/en
Publication of WO2002018952A2 publication Critical patent/WO2002018952A2/en
Publication of WO2002018952A3 publication Critical patent/WO2002018952A3/en

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366Apparatus specially adapted for solid-phase testing
    • G01N33/54373Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366Apparatus specially adapted for solid-phase testing
    • G01N33/54373Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438Electrodes

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

A diagnostic method and device for use in detecting or quantitating an analyte present in a liquid sample. The method includes reacting an analyte-containing sample with reagents capable of generating a first coil-forming peptide in solution form. This peptide is then contacted with a biosensor whose detection surface has surface-bound molecules of a second, oppositely charged coil-forming peptide, under conditions effective to form a stable α-helical coiled-coil heterodimer on the detection surface. The formation of the coiled-coil heterodimer produces a measurable change in biosensor signal, when is measured to detect the presence of or quantitate the amount of analyte in a sample.
PCT/CA2001/001246 2000-09-01 2001-08-30 Biosensor assay device and method WO2002018952A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP01966907A EP1314036A2 (en) 2000-09-01 2001-08-30 Biosensor assay device and method
AU2001287451A AU2001287451A1 (en) 2000-09-01 2001-08-30 Biosensor assay device and method
CA002420743A CA2420743A1 (en) 2000-09-01 2001-08-30 Biosensor assay device and method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US65419100A 2000-09-01 2000-09-01
US09/654,191 2000-09-01

Publications (2)

Publication Number Publication Date
WO2002018952A2 WO2002018952A2 (en) 2002-03-07
WO2002018952A3 true WO2002018952A3 (en) 2002-10-24

Family

ID=24623825

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2001/001246 WO2002018952A2 (en) 2000-09-01 2001-08-30 Biosensor assay device and method

Country Status (4)

Country Link
EP (1) EP1314036A2 (en)
AU (1) AU2001287451A1 (en)
CA (1) CA2420743A1 (en)
WO (1) WO2002018952A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10200161A1 (en) * 2002-01-04 2003-07-24 Herbert P Jennissen Method for producing models of biological molecules, especially for use in the production of implants or prostheses, whereby the models are used on the surface of the implants so that they resemble biological molecules
GB2422664A (en) * 2005-01-28 2006-08-02 Ethicon Inc Device for detecting an enzyme in a sample
US8822206B2 (en) * 2005-10-11 2014-09-02 The Johns Hopkins University Device for high-throughput stimulation, immunostaining, and visualization of single cells
WO2008092041A2 (en) 2007-01-24 2008-07-31 Carnegie Mellon University Optical biosensors
GB2453356A (en) * 2007-10-03 2009-04-08 James Homrig Assay device comprising control analytes to confirm assay completion
CN102460139B (en) * 2009-06-08 2014-04-30 西安大略大学 An electrochemical method and apparatus of identifying the presence of a target

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997041424A1 (en) * 1996-04-25 1997-11-06 Pence, Inc. Biosensor device and method
WO2000052456A1 (en) * 1999-03-02 2000-09-08 Helix Biopharma Corporation Biosensor device and method

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997041424A1 (en) * 1996-04-25 1997-11-06 Pence, Inc. Biosensor device and method
WO2000052456A1 (en) * 1999-03-02 2000-09-08 Helix Biopharma Corporation Biosensor device and method

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHAO H ET AL: "Use of a heterodimeric coiled-coil system for biosensor application and affinity purification", JOURNAL OF CHROMATOGRAPHY B: BIOMEDICAL SCIENCES & APPLICATIONS, ELSEVIER SCIENCE PUBLISHERS, NL, vol. 715, no. 1, 11 September 1998 (1998-09-11), pages 307 - 329, XP004147004, ISSN: 1570-0232 *
CHAO HEMAN ET AL: "Kinetic study on the formation of a de novo designed heterodimeric coiled-coil: Use of surface plasmon resonance to monitor the association and dissociation of polypeptide chains", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY. EASTON, PA, US, vol. 35, no. 37, 1996, pages 12175 - 12185, XP002140237, ISSN: 0006-2960 *
HODGES R S: "DE NOVO DESIGN OF ALPHA-HELICAL PROTEINS: BASIC RESEARCH TO MEDICALAPPLICATIONS", BIOCHEMISTRY AND CELL BIOLOGY. BIOCHIMIE ET BIOLOGIE CELLULAIRE, XX, XX, vol. 74, no. 2, 1996, pages 133 - 154, XP000605834, ISSN: 0829-8211 *

Also Published As

Publication number Publication date
CA2420743A1 (en) 2002-03-07
EP1314036A2 (en) 2003-05-28
WO2002018952A2 (en) 2002-03-07
AU2001287451A1 (en) 2002-03-13

Similar Documents

Publication Publication Date Title
EP1111386A3 (en) Test strip for the assay of an analyte in a liquid sample
US20030119208A1 (en) Electrochemical immunosensor and kit and method for detecting biochemical anylyte using the sensor
CA2152498A1 (en) Method, test article, and system for performing assays
WO2001090749A3 (en) Scanning kelvin microprobe system and process for biomolecule microassay
CA2218008A1 (en) Use of thermochromic liquid crystals in reflectometry based diagnostic methods
NZ306051A (en) Testing using electrochemiluminescence
WO2001038873A3 (en) Devices and methods for detecting analytes using electrosensor having capture reagent
CA2250242A1 (en) Quantitative immunochromatographic assays
AU2003238869A1 (en) Hybrid microcantilever sensors
EP1318399A3 (en) Passive sample detection to initiate timing of an assay
WO2004094986A3 (en) System and method for electrochemical detection of biological compounds
WO2001036681A3 (en) Immunological detection of rna:dna hybrids on microarrays
CA2331688A1 (en) Detection of a target in a sample
WO2002004668A3 (en) Improved capture and detection of target nucleic acid in dipstick assays
EP0759555A3 (en) Direct-reading reagent test strip
WO2023018730A9 (en) Detection of analytes using targeted epigenetic assays, proximity-induced tagmentation, strand invasion, restriction, or ligation
AU2004227171A1 (en) Optical chemical sensing device with pyroelectric or piezoelectric transducer
AU8029991A (en) Conductive sensors and their use in diagnostic assays
WO2002004670A3 (en) Improved capture and detection of target nucleic acid in dipstick assays
WO2000043774A3 (en) Detection of small molecules by use of a piezoelectric sensor
WO2002014535A3 (en) Electrochemical strip test for small volumes
CA2341263A1 (en) Enzyme inhibition immunoassay
WO2002018952A3 (en) Biosensor assay device and method
WO2008122960A3 (en) Label-free optical detection method
WO2005073405A3 (en) Catalytic polynucleotide and its use for determination of analytes

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PH PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PH PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 2420743

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2001287451

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2001966907

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2001966907

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWW Wipo information: withdrawn in national office

Ref document number: 2001966907

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: JP