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WO2001092888A3 - A method for screening and isolating microorganisms - Google Patents

A method for screening and isolating microorganisms Download PDF

Info

Publication number
WO2001092888A3
WO2001092888A3 PCT/EP2001/006123 EP0106123W WO0192888A3 WO 2001092888 A3 WO2001092888 A3 WO 2001092888A3 EP 0106123 W EP0106123 W EP 0106123W WO 0192888 A3 WO0192888 A3 WO 0192888A3
Authority
WO
WIPO (PCT)
Prior art keywords
antigen
microorganisms
suspension
antibody
antibodies
Prior art date
Application number
PCT/EP2001/006123
Other languages
French (fr)
Other versions
WO2001092888A2 (en
Inventor
Markus Hanner
Markus Horky
Gabain Alexander Von
Ahsen Uwe Von
Original Assignee
Cistem Biotechnologies Gmbh
Markus Hanner
Markus Horky
Gabain Alexander Von
Ahsen Uwe
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cistem Biotechnologies Gmbh, Markus Hanner, Markus Horky, Gabain Alexander Von, Ahsen Uwe filed Critical Cistem Biotechnologies Gmbh
Priority to AU8382501A priority Critical patent/AU8382501A/en
Publication of WO2001092888A2 publication Critical patent/WO2001092888A2/en
Publication of WO2001092888A3 publication Critical patent/WO2001092888A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Described is a method for screening an isolating microorganisms, especially prokaryotic and eukaryotic cells, displaying an antigen, which method is characterized by the following steps: providing a suspension of a multiplicity of viable microorganisms containing one or more microorganisms, especially prokaryotic and eukaryotic cells, displaying at least one type of antigen on their surface, contacting said suspension of microorganisms with a solution containing antibodies, which bind to said at least one type of antigen, incubating said suspension with said antibodies to allow binding of the antibodies to the antigen(s), contacting said suspension of microorganisms with antibody/antigen complexes on their surface with C1q molecules to allow binding of the C1q molecules to the surface located antibody/antigen complexes and isolating viable microorganisms, especially prokaryotic and eukaryotic cel ls, with exhibit C1q molecules bound to an antibody/antigen complex on their surface.
PCT/EP2001/006123 2000-05-31 2001-05-30 A method for screening and isolating microorganisms WO2001092888A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU8382501A AU8382501A (en) 2000-05-31 2001-05-30 A method for screening and isolating microorganisms, especially prokaryotic and eukaryotic cells, displaying an antigen

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ATA961/2000 2000-05-31
AT9612000A AT409801B (en) 2000-05-31 2000-05-31 METHOD FOR SCREENING AND ISOLATING MICROORGANISMS, IN PARTICULAR PROKARYONTIC AND EUKARYONTIC CELLS THAT PRESENT AN ANTIQUE

Publications (2)

Publication Number Publication Date
WO2001092888A2 WO2001092888A2 (en) 2001-12-06
WO2001092888A3 true WO2001092888A3 (en) 2002-07-04

Family

ID=3683572

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/006123 WO2001092888A2 (en) 2000-05-31 2001-05-30 A method for screening and isolating microorganisms

Country Status (3)

Country Link
AT (1) AT409801B (en)
AU (1) AU8382501A (en)
WO (1) WO2001092888A2 (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4062935A (en) * 1974-05-20 1977-12-13 Technicon Instruments Corporation Immunoassay involving the binding of RF to the antigen-antibody complex
US4307190A (en) * 1978-10-30 1981-12-22 Technicon Instruments Corporation Immunoassay using ascitic fluid
US4882423A (en) * 1984-10-02 1989-11-21 Calpis Food Industry Substance-conjugated complement component C1q
US5035995A (en) * 1984-10-02 1991-07-30 Calpis Food Industry Co., Ltd. Test method involving substance-conjugated complement component C1q
JPH03233358A (en) * 1990-02-08 1991-10-17 Internatl Reagents Corp Method for measuring antigen or antibody with high sensitivity
WO1996021675A2 (en) * 1995-01-13 1996-07-18 Innogenetics N.V. Capturing of microorganisms using complement components

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4551435A (en) * 1983-08-24 1985-11-05 Immunicon, Inc. Selective removal of immunospecifically recognizable substances from solution
EP0152254A3 (en) * 1984-02-06 1986-08-06 E.I. Du Pont De Nemours And Company Chromogenic support immunoassay utilizing labeled complement components
EP0177023A3 (en) * 1984-10-02 1987-08-12 The Calpis Food Industry Co., Ltd. Substance-conjugated complement component c1q
ATE77700T1 (en) * 1986-03-10 1992-07-15 Imre Corp IMMUNOCOMPLEX TEST PROCEDURE.
US4925788A (en) * 1986-10-24 1990-05-15 Immunicon Corporation Immunoassay system and procedure based on precipitin-like interaction between immune complex and Clq or other non-immunospecific factor
US6013532A (en) * 1990-09-26 2000-01-11 Immunivest Corporation Methods for magnetic immobilization and manipulation of cells
US5466574A (en) * 1991-03-25 1995-11-14 Immunivest Corporation Apparatus and methods for magnetic separation featuring external magnetic means

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4062935A (en) * 1974-05-20 1977-12-13 Technicon Instruments Corporation Immunoassay involving the binding of RF to the antigen-antibody complex
US4307190A (en) * 1978-10-30 1981-12-22 Technicon Instruments Corporation Immunoassay using ascitic fluid
US4882423A (en) * 1984-10-02 1989-11-21 Calpis Food Industry Substance-conjugated complement component C1q
US5035995A (en) * 1984-10-02 1991-07-30 Calpis Food Industry Co., Ltd. Test method involving substance-conjugated complement component C1q
JPH03233358A (en) * 1990-02-08 1991-10-17 Internatl Reagents Corp Method for measuring antigen or antibody with high sensitivity
WO1996021675A2 (en) * 1995-01-13 1996-07-18 Innogenetics N.V. Capturing of microorganisms using complement components

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PATENT ABSTRACTS OF JAPAN vol. 016, no. 015 (P - 1298) 14 January 1992 (1992-01-14) *

Also Published As

Publication number Publication date
AU8382501A (en) 2001-12-11
WO2001092888A2 (en) 2001-12-06
ATA9612000A (en) 2002-03-15
AT409801B (en) 2002-11-25

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