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WO2001092569A3 - Formulation for polymerase chain reaction and vessel containing same - Google Patents

Formulation for polymerase chain reaction and vessel containing same Download PDF

Info

Publication number
WO2001092569A3
WO2001092569A3 PCT/GB2001/002265 GB0102265W WO0192569A3 WO 2001092569 A3 WO2001092569 A3 WO 2001092569A3 GB 0102265 W GB0102265 W GB 0102265W WO 0192569 A3 WO0192569 A3 WO 0192569A3
Authority
WO
WIPO (PCT)
Prior art keywords
formulation
chain reaction
polymerase chain
vessel containing
containing same
Prior art date
Application number
PCT/GB2001/002265
Other languages
French (fr)
Other versions
WO2001092569A2 (en
Inventor
John Douglas Oultram
Conor Joseph Mulrooney
Jacqueline Clare Coutts
Original Assignee
Tepnel Medical Ltd
John Douglas Oultram
Conor Joseph Mulrooney
Jacqueline Clare Coutts
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0013043A external-priority patent/GB0013043D0/en
Priority claimed from GB0013863A external-priority patent/GB0013863D0/en
Application filed by Tepnel Medical Ltd, John Douglas Oultram, Conor Joseph Mulrooney, Jacqueline Clare Coutts filed Critical Tepnel Medical Ltd
Priority to AU2001262483A priority Critical patent/AU2001262483A1/en
Priority to EP01936610A priority patent/EP1290223A2/en
Publication of WO2001092569A2 publication Critical patent/WO2001092569A2/en
Publication of WO2001092569A3 publication Critical patent/WO2001092569A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A formulation for use in effecting a Polymerase Chain Reaction, a dried composition of reagents including reaction buffer, dNTPs, at least two primers and a polymerase. The formulation is re-hydratable to be capable of effecting amplification of a target nucleic acid sequence of interest. The formulation incorporates a fluorescent reporter molecule capable of reporting by homologous detection the presence of amplified nucleic acid produced by the Polymerase Chain Reaction.
PCT/GB2001/002265 2000-05-31 2001-05-23 Formulation for polymerase chain reaction and vessel containing same WO2001092569A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2001262483A AU2001262483A1 (en) 2000-05-31 2001-05-23 Formulation for polymerase chain reaction and vessel containing same
EP01936610A EP1290223A2 (en) 2000-05-31 2001-05-23 Formulation for polymerase chain reaction and vessel containing same

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB0013043.5 2000-05-31
GB0013043A GB0013043D0 (en) 2000-05-31 2000-05-31 Formulation for polymerase chain reaction and vessel containing same
GB0013863.6 2000-06-07
GB0013863A GB0013863D0 (en) 2000-06-07 2000-06-07 Formulation for polymerase chain reaction and vessel containing same

Publications (2)

Publication Number Publication Date
WO2001092569A2 WO2001092569A2 (en) 2001-12-06
WO2001092569A3 true WO2001092569A3 (en) 2002-03-28

Family

ID=26244374

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2001/002265 WO2001092569A2 (en) 2000-05-31 2001-05-23 Formulation for polymerase chain reaction and vessel containing same

Country Status (3)

Country Link
EP (1) EP1290223A2 (en)
AU (1) AU2001262483A1 (en)
WO (1) WO2001092569A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11845081B2 (en) 2007-07-13 2023-12-19 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030186252A1 (en) * 2002-04-01 2003-10-02 Ilsley Diane D. Array based hybridization assays employing enzymatically generated labeled target nucleic acids and compositions for practicing the same
ATE531817T1 (en) * 2003-07-15 2011-11-15 Lukas Bestmann SAMPLE PREPARATION UNIT
WO2005011867A2 (en) 2003-07-31 2005-02-10 Handylab, Inc. Processing particle-containing samples
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
FI20040768A0 (en) * 2004-06-04 2004-06-04 Teemu Korpimaeki Method for stabilizing assay reagents, reagent tank containing stabilized assay reagents and its use
US7776530B2 (en) 2004-06-29 2010-08-17 Wallac Oy Integrated nucleic acid analysis
FI20045248A7 (en) * 2004-06-29 2005-12-30 Wallac Oy Integrated nucleic acid analysis
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
DK3088083T3 (en) 2006-03-24 2018-11-26 Handylab Inc Method of carrying out PCR down a multi-track cartridge
US8883490B2 (en) 2006-03-24 2014-11-11 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
EP2021508B1 (en) * 2006-05-23 2012-04-25 Molecular Detection, Inc. Ambient temperature stable kits for molecular diagnostics
US8709787B2 (en) 2006-11-14 2014-04-29 Handylab, Inc. Microfluidic cartridge and method of using same
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
EP2171460B1 (en) 2007-07-13 2017-08-30 Handylab, Inc. Polynucleotide capture materials, and methods of using same
DE102010038330A1 (en) * 2010-07-23 2012-03-01 Aj Innuscreen Gmbh Method, device and test kit for molecular biological reactions
AU2012242510B2 (en) 2011-04-15 2015-05-14 Becton, Dickinson And Company Scanning real-time microfluidic thermocycler and methods for synchronized thermocycling and scanning optical detection
EP2574931B1 (en) 2011-09-29 2017-03-22 Qiagen GmbH Dry composition comprising a control dye
WO2013053855A1 (en) 2011-10-11 2013-04-18 Qiagen Gmbh Sample processing method and sample processing cartridge
WO2013067202A1 (en) 2011-11-04 2013-05-10 Handylab, Inc. Polynucleotide sample preparation device
WO2013068107A1 (en) 2011-11-07 2013-05-16 Qiagen Gmbh Lysis method and lysis composition
EP2730653A1 (en) 2012-11-07 2014-05-14 QIAGEN GmbH Method for lysing a fixed biological sample

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0512334A2 (en) * 1991-05-02 1992-11-11 F. Hoffmann-La Roche Ag Methods for detecting a target nucleic acid in a sample
EP0674009A2 (en) * 1994-03-14 1995-09-27 Becton, Dickinson and Company Nucleic acid amplification method and apparatus
WO1997010056A2 (en) * 1995-09-12 1997-03-20 Becton Dickinson And Company Device and method for dna amplification and assay
EP0834729A2 (en) * 1996-09-26 1998-04-08 Becton, Dickinson and Company DNA microwell device and method
US5861251A (en) * 1996-10-15 1999-01-19 Bioneer Corporation Lyophilized reagent for polymerase chain reaction

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0512334A2 (en) * 1991-05-02 1992-11-11 F. Hoffmann-La Roche Ag Methods for detecting a target nucleic acid in a sample
EP0674009A2 (en) * 1994-03-14 1995-09-27 Becton, Dickinson and Company Nucleic acid amplification method and apparatus
WO1997010056A2 (en) * 1995-09-12 1997-03-20 Becton Dickinson And Company Device and method for dna amplification and assay
EP0834729A2 (en) * 1996-09-26 1998-04-08 Becton, Dickinson and Company DNA microwell device and method
US5861251A (en) * 1996-10-15 1999-01-19 Bioneer Corporation Lyophilized reagent for polymerase chain reaction

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11845081B2 (en) 2007-07-13 2023-12-19 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples

Also Published As

Publication number Publication date
WO2001092569A2 (en) 2001-12-06
AU2001262483A1 (en) 2001-12-11
EP1290223A2 (en) 2003-03-12

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