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WO2001047958A3 - Kunitz domain polypeptide zkun10 - Google Patents

Kunitz domain polypeptide zkun10 Download PDF

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Publication number
WO2001047958A3
WO2001047958A3 PCT/US2000/035709 US0035709W WO0147958A3 WO 2001047958 A3 WO2001047958 A3 WO 2001047958A3 US 0035709 W US0035709 W US 0035709W WO 0147958 A3 WO0147958 A3 WO 0147958A3
Authority
WO
WIPO (PCT)
Prior art keywords
kunitz domain
seq
domain
zkun10
proteinase inhibitors
Prior art date
Application number
PCT/US2000/035709
Other languages
French (fr)
Other versions
WO2001047958A2 (en
Inventor
Paul O Sheppard
Brian A Fox
Original Assignee
Zymogenetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zymogenetics Inc filed Critical Zymogenetics Inc
Priority to AU22963/01A priority Critical patent/AU2296301A/en
Publication of WO2001047958A2 publication Critical patent/WO2001047958A2/en
Publication of WO2001047958A3 publication Critical patent/WO2001047958A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8114—Kunitz type inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/022—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from an adenovirus

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Proteinase inhibitors comprising a Kunitz domain are disclosed. The Kunitz domain comprises a motif of amino acid residues as shown in SEQ ID NO:4, and the sequence of the Kunitz domain is shown in residues 57 through 107 of SEQ ID NO:2. The polypeptide also includes an N-terminal collagen domain in which a von Willebrand domain resides, and is shown in SEQ ID NO:5. Also disclosed are methods for making the proteinase inhibitors, and expression vectors and cultured cells that are useful within the methods. The proteinase inhibitors may be used as components of cell culture media, in protein purification, and as inhibitors of protease degradation of plasma proteins.
PCT/US2000/035709 1999-12-29 2000-12-28 Kunitz domain polypeptide zkun10 WO2001047958A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU22963/01A AU2296301A (en) 1999-12-29 2000-12-28 Kunitz domain polypeptide zkun10

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US47428099A 1999-12-29 1999-12-29
US09/474,280 1999-12-29

Publications (2)

Publication Number Publication Date
WO2001047958A2 WO2001047958A2 (en) 2001-07-05
WO2001047958A3 true WO2001047958A3 (en) 2002-01-03

Family

ID=23882870

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/035709 WO2001047958A2 (en) 1999-12-29 2000-12-28 Kunitz domain polypeptide zkun10

Country Status (2)

Country Link
AU (1) AU2296301A (en)
WO (1) WO2001047958A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1471933A1 (en) * 2002-02-07 2004-11-03 Delta Biotechnology Limited Albumin-fused anti-angiogenesis peptides

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995021601A2 (en) * 1994-01-11 1995-08-17 Protein Engineering Corporation Kallikrein-inhibiting 'kunitz domain' proteins and analogues thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995021601A2 (en) * 1994-01-11 1995-08-17 Protein Engineering Corporation Kallikrein-inhibiting 'kunitz domain' proteins and analogues thereof

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BONALDO P ET AL: "The carboxyl terminus of the chicken alpha 3 chain of collagen VI is a unique mosaic structure with glycoprotein Ib-like, fibronectin type III, and Kunitz modules.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 264, no. 34, 5 December 1989 (1989-12-05), pages 20235 - 20239, XP001008010 *
DATABASE AAGENESEQ DERWENT; 18 March 1996 (1996-03-18), LADNER R.C. AND MARKLAND W.: "Human collagen alpha-3 Kunitz domain.", XP002171881 *
DATABASE EMBL EBI; 10 March 1998 (1998-03-10), STRAUSBERG R.: "ai84h04.s1 Soares_parathyroid_tumor_NbHPA Homo sapiens cDNA clone", XP002171882 *
DATABASE EMBL EBI; 9 September 1998 (1998-09-09), MAHAIRAS G.G. ET AL.: "HS_3151_B1_A11_T7 CIT Approved Human Genomic Sperm Library D Homo sapiens genomic clone Plate=3151 Col=21 Row=B, genomic survey sequence", XP002171883 *
DATABASE SWISSPROT 1 April 1990 (1990-04-01), DOLIANA R. ET AL.: "COLLAGEN ALPHA 3(VI) CHAIN PRECURSOR", XP002171880 *
MAYER U ET AL: "Recombinant expression and properties of the Kunitz -type protease-inhibitor module from human type VI collagen alpha 3(VI) chain.", EUROPEAN JOURNAL OF BIOCHEMISTRY, (1994 OCT 15) 225 (2) 573-80., XP000999464 *

Also Published As

Publication number Publication date
WO2001047958A2 (en) 2001-07-05
AU2296301A (en) 2001-07-09

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