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WO2001046688A1 - Mecanisme de couvercle - Google Patents

Mecanisme de couvercle Download PDF

Info

Publication number
WO2001046688A1
WO2001046688A1 PCT/SG1999/000149 SG9900149W WO0146688A1 WO 2001046688 A1 WO2001046688 A1 WO 2001046688A1 SG 9900149 W SG9900149 W SG 9900149W WO 0146688 A1 WO0146688 A1 WO 0146688A1
Authority
WO
WIPO (PCT)
Prior art keywords
lid
sample
closed position
drive
mechanism according
Prior art date
Application number
PCT/SG1999/000149
Other languages
English (en)
Inventor
Hon Siu Shin
Original Assignee
Pe Holding B.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pe Holding B.V. filed Critical Pe Holding B.V.
Priority to AU30961/00A priority Critical patent/AU3096100A/en
Priority to PCT/SG1999/000149 priority patent/WO2001046688A1/fr
Priority to EP99964935A priority patent/EP1456642A1/fr
Publication of WO2001046688A1 publication Critical patent/WO2001046688A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

Definitions

  • the invention relates to a lid mechanism for laboratory equipment, for example, polymerisation chain reaction thermal cycle apparatus.
  • Polymerisation chain reaction thermal cycle apparatus is commonly used when analysing DNA.
  • the apparatus typically includes a sample tray that has a number of recessed walls into which samples are introduced.
  • the sample tray is then positioned in a sample block in the apparatus and the lid, which has a heating plate on its underside, is pressed down onto the sample tray to clamp the sample tray between the sample block and the heating plate.
  • the sample block is then heated cyclically over a period of time to cause a polymerisation chain reaction within the sample.
  • the lid is removed from the sample tray.
  • the sample tray is then removed from the sample block and the samples within the sample tray are analysed.
  • a lid mechanism for laboratory equipment comprising a lid movable between an open position in which a sample may be inserted into or removed from the equipment, and a closed position in which a sample is prevented from being removed from the equipment; and a drive mechanism coupled to the lid and operable to move the lid between the open and the closed position.
  • An advantage of the invention is that by providing a lid mechanism which includes a drive mechanism it is possible to provide laboratory equipment, such as polymerisation chain reaction thermal cycle apparatus, with a lid which can be opened and closed without requiring manual intervention.
  • the laboratory equipment is polymerisation chain reaction thermal cycle apparatus.
  • the underside of the lid is pressed into engagement with a sample holder, in which a sample is located.
  • the lid when the lid is in the open position, the lid is displaced from the sample holder in a vertical and a horizontal direction.
  • the drive mechanism comprises a rotating screw drive which rotates to move the lid in a linear motion.
  • the lid when the lid is moved from the open to the closed position, the lid is moved in a first direction towards the sample holder to an intermediate position in which the lid is located above the sample holder and the drive mechanism then moves the lid in a second direction to move the lid from the intermediate position to the closed position.
  • the lid includes a planar surface on the underside.
  • the sample holder is located in a sample block and when the lid is in the closed position, the sample holder is clamped between the sample block and the planar surface.
  • the sample block and/or the planar surface may be heated to enable heating of a sample in the sample holder.
  • the sample block and/or the planar surface are metallic.
  • the drive mechanism further comprises a cam follower arrangement to move the lid from the intermediate position to the closed position.
  • the lid may be pivoted with respect to the drive mechanism to permit access to the underside of the lid, for
  • Figure 1 is a perspective view of a polymerisation chain reaction thermal
  • Figure 2 is a top view of the lid block module of Figure 1 ;
  • Figure 3 is a cross-sectional view of the lid block module along the line
  • FIG 4 is a side view of the lid block module shown in Figure 1 with a
  • Figure 5 is an enlarged view of the partial cross-sectional shown in
  • Figure 6 is a perspective view of a cam and slide plate forming part of the
  • Figure 7 is a rear view of the lid block module shown in Figure 1 ;
  • FIG 8 is a perspective view of the lid block module shown in Figure 1
  • Figure 9 is a perspective view of the lid block module with the lid in an
  • FIG 10 is a top view of the lid block module shown in Figure 9;
  • Figure 1 1 is a cross-sectional view along the line CC of Figure 10;
  • Figure 12 is a side view of the lid block module of Figure 10 with a partial cross-sectional view along the line DD of Figure 10 showing the cam mechanism;
  • Figure 13 is an enlarged view of the partial cross-sectional view shown in Figure 12;
  • Figure 14 is a perspective view of the lid block module showing the lid in a closed position
  • Figure 15 is a top view of the lid module shown in Figure 14;
  • Figure 16 is a cross-sectional view along the line EE of Figure 15;
  • Figure 17 is a side view of the lid block module of Figure 15 with a partial cross-sectional view along the line FF of Figure 15 showing the cam mechanism; and
  • Figure 18 is an enlarged view of the partial cross-sectional view shown in Figure 17.
  • FIG. 1 shows a perspective view of a lid block module 1 for polymerisation chain reaction thermal cycle apparatus.
  • the lid block module 1 includes a base 2 with two sample blocks 3 mounted thereon. Also mounted on the base 2 is a drive plate 4 which supports two lead screws 5.
  • Each lead screw 5 includes a drive nut 6 which engages with a cam 7, 8 mounted on the side of a lid (or heated cover) 9.
  • the lid module 1 plugs into and is supported on a base module (not shown).
  • the base module includes firmware and a control system to control the heating and thermal coupling to the sample blocks 3 and also provides heating to platen 10 (see Figure 3) located on the underside of the lid 9.
  • the lid 9 includes an outer cover 11. Within the outer cover 11 is located a support member 12 which extends between two pins 13 which extend out of the side of the cover 11 and engage in a cam profile 40 in each of the cams 7, 8.
  • the platens 10 are mounted on the support member 12 by means of a base plate 14 attached to each platen 10 and mounting members 15 which couple the base plates to the support member 12.
  • Figure 3 also shows pivotable rear guards 16, 17 that cover each of the lead screws 5. The rear guards 16, 17 are not shown in Figures 1 and 2 for clarity.
  • FIG. 4 A side view of the lid block module 1 is shown in Figure 4 in which a cut away section shows a cross-sectional view of the cam mechanism along the line BB of Figure 2. Also shown in Figure 4 are cooling fins 47 that extend from the bottom of the sample blocks 3.
  • FIG. 5 An enlarged view of the cross-sectional view of the cam mechanism is shown in Figure 5.
  • the cam 7 slidingly engages with a right hand side slide plate 18.
  • a perspective view of the right cam 7 and the right slide plate 18 is shown in Figure 6.
  • the left cam 8 is symmetrically opposite to the right cam 7.
  • the right slide plate 18 has two holes 19 that permit the slide plate 18 to be fixed to the right hand side of the external cover 11.
  • the right cam 7 is able to slide with respect to the lid 1 and the right slide plate 18 due to the slide grooves 20, 21 in the right cam 7 and the right slide plate 18, respectively.
  • a symmetrically opposite left-hand slide plate 38 is provided which engages with the left cam 8 in a similar manner.
  • the slide plate 18 also includes a spring-loaded interlocking shaft 22 that is biased by a spring 24 within the slide plate 18 to the position shown in Figure 5.
  • the position of the interlocking shaft 22 as shown in Figure 5 is when the spring 24 is in a fully extended position and end 25 of the shaft 22 protrudes above surface 26 of the slide plate 18.
  • the shaft 22 is limited by the amount that end 25 can protrude above surface 26 by a circlip 27.
  • the surface 23 of the cam 7 has been pushed over the end 25 of the shaft 22 thereby depressing the shaft 22 to compress the spring 24 so that the interlocking shaft 22 moves to the position shown in Figure 6.
  • Figure 7 shows a rear view of the lid module.
  • a drive wheel 26 around which is entrained a drive belt 27.
  • Mounted on the drive plate 4 are two guide pulleys 28 and a motor 29.
  • the motor 29 includes a drive shaft 30 on which is mounted a motor gear 31.
  • the motor gear 31 drives the belt 27 to rotate the drive wheels 26 so as to cause rotation of the lead screws 5.
  • the motor 29 is reversible so that the drive shaft 30 and the motor gear 31 (and therefore, the lead screws 5) may be driven in either direction.
  • Each of the drive nuts 6 is shaped so that it has a flatted side portion which butts against the side of the cams 7, 8. Therefore, on rotation of the lead screws 5, the drive nuts 6 are prevented from rotating so that they move along the lead screws 5 to move the lid 9 towards or away from the drive plate 4 depending on the direction of rotation of the lead screws 5 which is determined by the direction of rotation of the motor 29.
  • Figures 9 and 10 show the lid 9 in an intermediate position after it has been moved by rotation of the lead screws 5, by means of the motor 29 and the drive belt 27, from the open position shown in Figures 1 and 2.
  • the lid 9 is located above the sample blocks 3 and the platen 10 are in the positions shown in Figure 11. In this position, the lid 9 has been moved as far away as possible from the drive plate 4 and further movement away from the drive plate 4 is prevented by the cover 11 hitting a stop member (not shown).
  • the pins 13 are moved downward due to the forward movement of the cams 7, 8 to the position shown in Figures 14 to 18 in which the cams 7, 8 have been moved forward by the lead screws 5 as far as possible.
  • the interlocking shaft 22 is locked in the depressed position which engages with the locking aperture 41 and the pins 13 have been moved fully downward by the cam profile 40.
  • the rear guards 16, 17 are pivotable to the position shown in Figure 8. This permits the lid 9 to be pivoted upward as shown in Figure 8 to permit access to the underside surfaces of the platen 10 to permit cleaning of the platen 10.
  • the lid block module 1 also includes two fixed front guards 32, 33 which are not pivotable and cover the front portions of the lead screws 5.
  • Advantages of the invention are that it permits motorised and robotic motion of the lid 9 between the open and closed positions without requiring human intervention. This has the advantage of facilitating 24 hour operation of the polymerisation chain reaction thermal cycle apparatus without requiring human intervention.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

L'invention concerne un mécanisme de couvercle (1) destiné à du matériel de laboratoire, et notamment à un appareil à cycle thermique d'amplification en chaîne par polymérase. Ce mécanisme (1) comprend un couvercle (9) mobile entre une position d'ouverture, dans laquelle il est possible d'insérer un échantillon dans l'appareil ou de l'en ôter, et une position de fermeture dans laquelle il est impossible de retirer un échantillon de l'appareil. Ce mécanisme (1) comprend également un mécanisme d'entraînement (5, 6, 7, 8) couplé au couvercle (9) et commandé pour déplacer le couvercle (9) entre la position d'ouverture et celle de fermeture.
PCT/SG1999/000149 1999-12-22 1999-12-22 Mecanisme de couvercle WO2001046688A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU30961/00A AU3096100A (en) 1999-12-22 1999-12-22 A lid mechanism
PCT/SG1999/000149 WO2001046688A1 (fr) 1999-12-22 1999-12-22 Mecanisme de couvercle
EP99964935A EP1456642A1 (fr) 1999-12-22 1999-12-22 Mecanisme de couvercle

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/SG1999/000149 WO2001046688A1 (fr) 1999-12-22 1999-12-22 Mecanisme de couvercle

Publications (1)

Publication Number Publication Date
WO2001046688A1 true WO2001046688A1 (fr) 2001-06-28

Family

ID=20430269

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/SG1999/000149 WO2001046688A1 (fr) 1999-12-22 1999-12-22 Mecanisme de couvercle

Country Status (3)

Country Link
EP (1) EP1456642A1 (fr)
AU (1) AU3096100A (fr)
WO (1) WO2001046688A1 (fr)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE20117661U1 (de) * 2001-10-29 2003-03-13 MWG-BIOTECH AG, 85560 Ebersberg Thermocyclervorrichtung
WO2016011162A1 (fr) * 2014-07-16 2016-01-21 Synthetic Genomics, Inc. Mécanisme de couvercle
EP3632568A1 (fr) * 2018-10-02 2020-04-08 Biometra GmbH Dispositif de traitement thermique des échantillons
CN111742223A (zh) * 2017-12-11 2020-10-02 株式会社百奥尼 聚合酶链反应系统
WO2021094362A1 (fr) * 2019-11-11 2021-05-20 Sharp Life Science (Eu) Limited Système microfluidique comprenant un dissipateur thermique à distance
US11110462B2 (en) * 2015-12-22 2021-09-07 Life Technologies Corporation Systems and methods for a thermal cycler heated cover
CN113966388A (zh) * 2019-04-11 2022-01-21 株式会社百奥尼 聚合酶链式反应系统
WO2023235843A3 (fr) * 2022-06-03 2024-02-08 10X Genomics, Inc. Procédés, systèmes et dispositifs pour interface d'échantillon

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5508197A (en) * 1994-07-25 1996-04-16 The Regents, University Of California High-speed thermal cycling system and method of use
US5795547A (en) * 1993-09-10 1998-08-18 Roche Diagnostic Systems, Inc. Thermal cycler
EP0955097A1 (fr) * 1998-05-04 1999-11-10 F. Hoffmann-La Roche Ag Dispositif de cycles thermiques avec positionnement automatique du couvercle

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5795547A (en) * 1993-09-10 1998-08-18 Roche Diagnostic Systems, Inc. Thermal cycler
US5508197A (en) * 1994-07-25 1996-04-16 The Regents, University Of California High-speed thermal cycling system and method of use
EP0955097A1 (fr) * 1998-05-04 1999-11-10 F. Hoffmann-La Roche Ag Dispositif de cycles thermiques avec positionnement automatique du couvercle

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE20117661U1 (de) * 2001-10-29 2003-03-13 MWG-BIOTECH AG, 85560 Ebersberg Thermocyclervorrichtung
WO2016011162A1 (fr) * 2014-07-16 2016-01-21 Synthetic Genomics, Inc. Mécanisme de couvercle
US9446407B2 (en) 2014-07-16 2016-09-20 Synthetic Genomics, Inc. Lid mechanism
US11110462B2 (en) * 2015-12-22 2021-09-07 Life Technologies Corporation Systems and methods for a thermal cycler heated cover
JP2021505204A (ja) * 2017-12-11 2021-02-18 バイオニア コーポレーションＢｉｏｎｅｅｒ Ｃｏｒｐｏｒａｔｉｏｎ ポリメラーゼ連鎖反応システム
CN111742223A (zh) * 2017-12-11 2020-10-02 株式会社百奥尼 聚合酶链反应系统
EP3726222A4 (fr) * 2017-12-11 2021-01-27 Bioneer Corporation Système de réaction en chaîne d'enzymes de polymérisation
US11247211B2 (en) * 2018-10-02 2022-02-15 Biometra GmbH Apparatus for the thermal treatment of samples
CN110975959A (zh) * 2018-10-02 2020-04-10 拜奥米特有限责任公司 用于样品的热处理的装置
EP3632568A1 (fr) * 2018-10-02 2020-04-08 Biometra GmbH Dispositif de traitement thermique des échantillons
CN110975959B (zh) * 2018-10-02 2022-06-10 拜奥米特有限责任公司 用于样品的热处理的装置
CN113966388A (zh) * 2019-04-11 2022-01-21 株式会社百奥尼 聚合酶链式反应系统
EP3954458A4 (fr) * 2019-04-11 2022-06-15 Bioneer Corporation Dispositif d'amplification en chaîne par polymérase
CN113966388B (zh) * 2019-04-11 2024-11-26 株式会社百奥尼 聚合酶链式反应系统
US12226768B2 (en) 2019-04-11 2025-02-18 Bioneer Corporation Polymerase chain reaction system
WO2021094362A1 (fr) * 2019-11-11 2021-05-20 Sharp Life Science (Eu) Limited Système microfluidique comprenant un dissipateur thermique à distance
US11235325B2 (en) 2019-11-11 2022-02-01 Sharp Life Science (Eu) Limited Microfluidic system including remote heat spreader
WO2023235843A3 (fr) * 2022-06-03 2024-02-08 10X Genomics, Inc. Procédés, systèmes et dispositifs pour interface d'échantillon

Also Published As

Publication number Publication date
AU3096100A (en) 2001-07-03
EP1456642A1 (fr) 2004-09-15

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