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WO2001034808A3 - Method of large-scale production and method of testing of the biological activity of a substance from soybean - Google Patents

Method of large-scale production and method of testing of the biological activity of a substance from soybean Download PDF

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Publication number
WO2001034808A3
WO2001034808A3 PCT/US2000/031211 US0031211W WO0134808A3 WO 2001034808 A3 WO2001034808 A3 WO 2001034808A3 US 0031211 W US0031211 W US 0031211W WO 0134808 A3 WO0134808 A3 WO 0134808A3
Authority
WO
WIPO (PCT)
Prior art keywords
biological activity
scale production
soybean
testing
substance
Prior art date
Application number
PCT/US2000/031211
Other languages
French (fr)
Other versions
WO2001034808A2 (en
WO2001034808A9 (en
Inventor
Alfredo F Galvez
Original Assignee
Filgen Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Filgen Biosciences Inc filed Critical Filgen Biosciences Inc
Priority to AU19183/01A priority Critical patent/AU1918301A/en
Publication of WO2001034808A2 publication Critical patent/WO2001034808A2/en
Publication of WO2001034808A3 publication Critical patent/WO2001034808A3/en
Publication of WO2001034808A9 publication Critical patent/WO2001034808A9/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The lunasin peptide has been shown to exhibit inhibitory effects against malignant transformation of cells induced by chemical carcinogens and viral oncogenes. The present invention relates to a method for large-scale production of lunasin using recombinant DNA technology. The invention also includes a method for a rapid, in vitro transformation assay to detect and quantify the biological activity of this cancer preventive peptide.
PCT/US2000/031211 1999-11-12 2000-11-12 Method of large-scale production and method of testing of the biological activity of a substance from soybean WO2001034808A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU19183/01A AU1918301A (en) 1999-11-12 2000-11-12 Method of large-scale production and method of testing of the biological activity of a substance from soybean

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16533499P 1999-11-12 1999-11-12
US60/165,334 1999-11-12

Publications (3)

Publication Number Publication Date
WO2001034808A2 WO2001034808A2 (en) 2001-05-17
WO2001034808A3 true WO2001034808A3 (en) 2002-07-04
WO2001034808A9 WO2001034808A9 (en) 2002-08-01

Family

ID=22598483

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/031211 WO2001034808A2 (en) 1999-11-12 2000-11-12 Method of large-scale production and method of testing of the biological activity of a substance from soybean

Country Status (2)

Country Link
AU (1) AU1918301A (en)
WO (1) WO2001034808A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030009013A1 (en) * 1998-12-30 2003-01-09 Genentech, Inc. Secreted and transmembrane polypeptides and nucleic acids encoding the same
CA2404100A1 (en) * 2000-03-24 2001-10-04 Filgen Biosciences, Inc. Therapeutic peptides having a motif that binds specifically to non-acetylated h3 and h4 histones for cancer therapy
US8598111B2 (en) 2006-09-15 2013-12-03 Soy Labs, Llc Products and methods using soy peptides to lower total and LDL cholesterol levels
US7731995B2 (en) 2006-09-16 2010-06-08 Alfredo Flores Galvez Methods for using soy peptides to inhibit H3 acetylation, reduce expression of HMG CoA reductase, and increase LDL receptor and Sp1 expression in a mammal
PL2061313T3 (en) 2006-09-16 2014-01-31 Soy Labs Llc Use of soy peptide (lunasin) to lower total and ldl cholesterol levels
US8759613B1 (en) 2009-10-26 2014-06-24 University Of Louisville Research Foundation, Inc. Method of producing a lunasin polypeptide in plants
US9066906B2 (en) 2009-11-11 2015-06-30 University Of Louisville Research Foundation, Inc. Lunasin-containing complex and purification of lunasin from plants
WO2014145086A2 (en) 2013-03-15 2014-09-18 Galvez Alfredo Flores Products and methods using lunasin-enriched soy extract mixtures to reduce free fatty acid levels, increase leptin levels and increase adiponectin levels in plasma
CN105647960A (en) * 2016-03-28 2016-06-08 中国农业科学院作物科学研究所 Inducible expression, purification and activity identification method of restructured lunasin polypeptide in pichia pastoris

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997035023A2 (en) * 1996-03-20 1997-09-25 Pioneer Hi-Bred International, Inc. Alteration of amino acid compositions in seeds
WO1999015642A1 (en) * 1997-09-25 1999-04-01 The Regents Of The University Of California Lunasin peptides
WO1999050671A2 (en) * 1998-03-30 1999-10-07 Rigel Pharmaceuticals, Inc. Toso as a target for drug screening

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997035023A2 (en) * 1996-03-20 1997-09-25 Pioneer Hi-Bred International, Inc. Alteration of amino acid compositions in seeds
WO1999015642A1 (en) * 1997-09-25 1999-04-01 The Regents Of The University Of California Lunasin peptides
WO1999050671A2 (en) * 1998-03-30 1999-10-07 Rigel Pharmaceuticals, Inc. Toso as a target for drug screening

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1996, GONZALEZ DE LA PENA MANUEL A ET AL: "Expression in Escherichia coli of Sin a 1, the major allergen from mustard.", XP002170665, Database accession no. PREV199698830349 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1997, HASSLACHER MEINHARD ET AL: "High-level intracellular expression of hydroxynitrile lyase from the tropical rubber tree Hevea brasiliensis in microbial host.", XP002170664, Database accession no. PREV199799798913 *
DATABASE EMBL 15 August 1997 (1997-08-15), GALVEZ A.F.,: "Glycine max 2S albumin pre-propeptide mRNA, complete cds.", XP002170663 *
EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 237, no. 3, 1996, pages 827 - 832, ISSN: 0014-2956 *
GALVEZ, A. F.: "A soybean cDNA encoding a chromatin-binding peptide inhibits mitosis of mammalian cells", NATURE BIOTECHNOLOGY, (1999) VOL. 17, NO. 5, PP. 495-500., May 1999 (1999-05-01), XP002170660 *
GALVEZ, A.F., ET AL.: "A Novel Methionine-rich Protein From Soybean Cotyledon: Cloning and Characterization of cDNA (Accession No. AF005030).", PLANT GENE REGISTER PGR97-103. PLANT PHYSIOLOGY 114:1567. (1997)., XP002170661 *
J M CREGG ET AL: "Recent Advances in the expression of foreign genes in Pichia pastoris", BIO/TECHNOLOGY,NATURE PUBLISHING CO. NEW YORK,US, vol. 11, August 1993 (1993-08-01), pages 905 - 910, XP002170662, ISSN: 0733-222X *
ODANI S ET AL: "AMINO ACID SEQUENCE OF A SOYBEAN (GLYCINE MAX) SEED POLYPEPTIDE HAVING A POLY(L-ASPARTIC ACID) STRUCTURE", JOURNAL OF BIOLOGICAL CHEMISTRY,US,AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, vol. 262, no. 22, 5 August 1987 (1987-08-05), pages 10502 - 10505, XP002035494, ISSN: 0021-9258 *
PROTEIN EXPRESSION AND PURIFICATION, vol. 11, no. 1, 1997, pages 61 - 71, ISSN: 1046-5928 *
SREEKRISHNA K ET AL: "Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris", GENE: AN INTERNATIONAL JOURNAL ON GENES AND GENOMES,GB,ELSEVIER SCIENCE PUBLISHERS, BARKING, vol. 190, no. 1, 29 April 1997 (1997-04-29), pages 55 - 62, XP004064384, ISSN: 0378-1119 *

Also Published As

Publication number Publication date
WO2001034808A2 (en) 2001-05-17
WO2001034808A9 (en) 2002-08-01
AU1918301A (en) 2001-06-06

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