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WO2000063422A3 - Detection of biological warfare agents - Google Patents

Detection of biological warfare agents Download PDF

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Publication number
WO2000063422A3
WO2000063422A3 PCT/US2000/008782 US0008782W WO0063422A3 WO 2000063422 A3 WO2000063422 A3 WO 2000063422A3 US 0008782 W US0008782 W US 0008782W WO 0063422 A3 WO0063422 A3 WO 0063422A3
Authority
WO
WIPO (PCT)
Prior art keywords
iii
acid
chelate
selectivity
hydroxyethyl
Prior art date
Application number
PCT/US2000/008782
Other languages
French (fr)
Other versions
WO2000063422A2 (en
Inventor
Krishnaswamy S Rajan
Stephen S Mainer
Original Assignee
Iit Res Inst
Krishnaswamy S Rajan
Stephen S Mainer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Iit Res Inst, Krishnaswamy S Rajan, Stephen S Mainer filed Critical Iit Res Inst
Priority to CA002370559A priority Critical patent/CA2370559A1/en
Priority to AU51225/00A priority patent/AU5122500A/en
Priority to JP2000612499A priority patent/JP2002541857A/en
Priority to EP00935822A priority patent/EP1175507A2/en
Publication of WO2000063422A2 publication Critical patent/WO2000063422A2/en
Publication of WO2000063422A3 publication Critical patent/WO2000063422A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Methods and devices are provided for the detection of bacterial agents such agents as Bacillus anthracis and Clostridium botulinum with high sensitivity and selectivity. More specifically, methods and devices are based on a phosphorescence-emission detection system using chelate-stabilized lantanides (e.g, Eu(III), Tb(III), and Sm(III)) to detect various spore-specific small organic molecules (e.g, dipicolinic acid, diaminopimelic acid, n-acetlymuramic acid, and the like). By careful selection of the chelating agent or ligand coordinated to the lanthanide, both high specificity and selectivity can be obtained. Examples of suitable and preferred sensor systems include N-(2-hydroxyethyl)ethylenediaminetriacetic acid (HEDTA) and N-(2-hydroxyethyl)iminodiacetic acid (HEIDA) combined with europium (III) and/or terbium (III). The chelate-stabilized lanthanides react with the spore-specific 'target' molecules to form a characteristically phosphorescent product which can then be detected.
PCT/US2000/008782 1999-04-19 2000-04-03 Detection of biological warfare agents WO2000063422A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002370559A CA2370559A1 (en) 1999-04-19 2000-04-03 Detection of biological warfare agents
AU51225/00A AU5122500A (en) 1999-04-19 2000-04-03 Detection of biological warfare agents
JP2000612499A JP2002541857A (en) 1999-04-19 2000-04-03 Detection of biological warfare agents
EP00935822A EP1175507A2 (en) 1999-04-19 2000-04-03 Detection of biological warfare agents

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13000999P 1999-04-19 1999-04-19
US60/130,009 1999-04-19

Publications (2)

Publication Number Publication Date
WO2000063422A2 WO2000063422A2 (en) 2000-10-26
WO2000063422A3 true WO2000063422A3 (en) 2001-08-16

Family

ID=22442619

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/008782 WO2000063422A2 (en) 1999-04-19 2000-04-03 Detection of biological warfare agents

Country Status (5)

Country Link
EP (1) EP1175507A2 (en)
JP (1) JP2002541857A (en)
AU (1) AU5122500A (en)
CA (1) CA2370559A1 (en)
WO (1) WO2000063422A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7105135B2 (en) 2001-10-16 2006-09-12 Lockheed Martin Corporation System and method for large scale detection of hazardous materials in the mail or in other objects
AU2002366448A1 (en) * 2001-11-30 2003-09-02 California Institute Of Technology An improvement in a method bacterial endospore quantification using lanthanide dipicolinate luminescence
WO2003065009A2 (en) * 2002-02-01 2003-08-07 California Institute Of Technology Methods and apparatus for assays of bacterial spores
JP4138331B2 (en) * 2002-02-22 2008-08-27 独立行政法人科学技術振興機構 Fluorescent sensor for phosphate ion and phosphorylated peptide
GB0215878D0 (en) * 2002-07-09 2002-08-14 Smart Holograms Ltd Cell detection
US7611862B2 (en) 2004-11-12 2009-11-03 California Institute Of Technology Method and apparatus for detecting and quantifying bacterial spores on a surface
US7563615B2 (en) 2005-04-15 2009-07-21 California Institute Of Technology Apparatus and method for automated monitoring of airborne bacterial spores
US20070117175A1 (en) * 2005-06-17 2007-05-24 Adrian Ponce Airborne bacterial spores as an indicator of biomass in an indoor environment
US7608419B2 (en) * 2003-11-13 2009-10-27 California Institute Of Technology Method and apparatus for detecting and quantifying bacterial spores on a surface
US8293696B2 (en) 2009-02-06 2012-10-23 Ecolab, Inc. Alkaline composition comprising a chelant mixture, including HEIDA, and method of producing same
US20130130272A1 (en) * 2010-07-31 2013-05-23 Advanced Biomedical Limited Method, reagent, and apparatus for detecting a chemical chelator
WO2017075552A1 (en) * 2015-10-30 2017-05-04 20/20 Genesystems Inc. Method for increasing available protein from endospores for detection purposes
EP3726211A1 (en) * 2019-04-18 2020-10-21 Université de Genève System and method for detecting the presence of spores in fields
WO2020260684A1 (en) * 2019-06-28 2020-12-30 Københavns Universitet An optical sensor
JP2025060189A (en) * 2023-09-29 2025-04-10 ダイキン工業株式会社 Microbial analysis system and microbial analysis method

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4259313A (en) * 1978-10-18 1981-03-31 Eastman Kodak Company Fluorescent labels
US4587223A (en) * 1982-09-13 1986-05-06 Wallac Oy Method for quantitative determination of a biospecific affinity reaction
WO1987007955A1 (en) * 1986-06-17 1987-12-30 Baxter Travenol Laboratories, Inc. Homogeneous fluoroassay methods employing fluorescent background rejection and water-soluble rare earth metal chelate fluorophores
EP0283289A2 (en) * 1987-03-20 1988-09-21 C.R. Bard, Inc. Excitation and detection apparatus for remote sensor connected by optical fiber
US5876960A (en) * 1997-08-11 1999-03-02 The United States Of America As Represented By The Secretary Of The Army Bacterial spore detection and quantification methods

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4259313A (en) * 1978-10-18 1981-03-31 Eastman Kodak Company Fluorescent labels
US4587223A (en) * 1982-09-13 1986-05-06 Wallac Oy Method for quantitative determination of a biospecific affinity reaction
WO1987007955A1 (en) * 1986-06-17 1987-12-30 Baxter Travenol Laboratories, Inc. Homogeneous fluoroassay methods employing fluorescent background rejection and water-soluble rare earth metal chelate fluorophores
EP0283289A2 (en) * 1987-03-20 1988-09-21 C.R. Bard, Inc. Excitation and detection apparatus for remote sensor connected by optical fiber
US5876960A (en) * 1997-08-11 1999-03-02 The United States Of America As Represented By The Secretary Of The Army Bacterial spore detection and quantification methods

Also Published As

Publication number Publication date
EP1175507A2 (en) 2002-01-30
WO2000063422A2 (en) 2000-10-26
AU5122500A (en) 2000-11-02
CA2370559A1 (en) 2000-10-26
JP2002541857A (en) 2002-12-10

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