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WO2000049410A3 - Lcm (laser capture microdissection) for cellular protein analysis - Google Patents

Lcm (laser capture microdissection) for cellular protein analysis Download PDF

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Publication number
WO2000049410A3
WO2000049410A3 PCT/US2000/004023 US0004023W WO0049410A3 WO 2000049410 A3 WO2000049410 A3 WO 2000049410A3 US 0004023 W US0004023 W US 0004023W WO 0049410 A3 WO0049410 A3 WO 0049410A3
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WO
WIPO (PCT)
Prior art keywords
cells
protein
analysis
methods
normal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2000/004023
Other languages
French (fr)
Other versions
WO2000049410A2 (en
WO2000049410A9 (en
Inventor
Lance A Liotta
Nicole Simone
Michael Emmert-Buck
Emmanuel F Petricoin Iii
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Institutes of Health NIH
US Department of Health and Human Services
Original Assignee
National Institutes of Health NIH
US Department of Health and Human Services
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Institutes of Health NIH, US Department of Health and Human Services filed Critical National Institutes of Health NIH
Priority to AU33671/00A priority Critical patent/AU772680B2/en
Priority to US09/913,667 priority patent/US6969614B1/en
Priority to EP00911845A priority patent/EP1153300A2/en
Priority to JP2000600101A priority patent/JP2002537561A/en
Priority to CA002359649A priority patent/CA2359649A1/en
Publication of WO2000049410A2 publication Critical patent/WO2000049410A2/en
Publication of WO2000049410A3 publication Critical patent/WO2000049410A3/en
Anticipated expiration legal-status Critical
Publication of WO2000049410A9 publication Critical patent/WO2000049410A9/en
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G01N2001/2833—Collecting samples on a sticky, tacky, adhesive surface
    • G01N2001/284—Collecting samples on a sticky, tacky, adhesive surface using local activation of adhesive, i.e. Laser Capture Microdissection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2550/00—Electrophoretic profiling, e.g. for proteome analysis
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

The present invention describes devices and methods for performing protein analysis on laser capture microdissected cells, which permits proteomic analysis on cells of different populations. Particular disclosed examples are analysis of normal versus malignant cells, or a comparison of differential protein expression in cells that are progressing from normal to malignant. The protein content of the microdissected cells may be analyzed using techniques such as immunoassays, 1D and 2D gel electrophoresis characterization, Western blotting, liquid chromatography quadrapole ion trap electrospray (LCQ-MS), Matrix Assisted Laser Desorption Ionization/Time of Flight (MALDI/TOF), and Surface Enhanced Laser Desorption Ionization Spectroscopy (SELDI). In addition to permitting direct comparison of qualitative and quantitative protein content of tumor cells and normal cells from the same tissue sample, the methods also allow for investigation of protein characteristics of tumor cells, such as binding ability and amino acid sequence, and differential expression of proteins in particular cell populations in response to drug treatment. The present methods also provide, through the use of protein fingerprinting, a rapid and reliable way to identify the source tissue of a tumor metastasis.
PCT/US2000/004023 1999-02-16 2000-02-16 Lcm (laser capture microdissection) for cellular protein analysis Ceased WO2000049410A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU33671/00A AU772680B2 (en) 1999-02-16 2000-02-16 LCM (Laser capture microdissection) for cellular protein analysis
US09/913,667 US6969614B1 (en) 1999-02-16 2000-02-16 Methods for the isolation and analysis of cellular protein content
EP00911845A EP1153300A2 (en) 1999-02-16 2000-02-16 Lcm (laser capture microdissection) for cellular protein analysis
JP2000600101A JP2002537561A (en) 1999-02-16 2000-02-16 Methods and apparatus for isolating and analyzing cellular protein components
CA002359649A CA2359649A1 (en) 1999-02-16 2000-02-16 Lcm (laser capture microdissection) for cellular protein analysis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12028899P 1999-02-16 1999-02-16
US60/120,288 1999-02-16

Publications (3)

Publication Number Publication Date
WO2000049410A2 WO2000049410A2 (en) 2000-08-24
WO2000049410A3 true WO2000049410A3 (en) 2001-03-08
WO2000049410A9 WO2000049410A9 (en) 2001-08-30

Family

ID=22389357

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/004023 Ceased WO2000049410A2 (en) 1999-02-16 2000-02-16 Lcm (laser capture microdissection) for cellular protein analysis

Country Status (5)

Country Link
EP (1) EP1153300A2 (en)
JP (1) JP2002537561A (en)
AU (1) AU772680B2 (en)
CA (1) CA2359649A1 (en)
WO (1) WO2000049410A2 (en)

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US7075640B2 (en) 1997-10-01 2006-07-11 Arcturus Bioscience, Inc. Consumable for laser capture microdissection
US7676328B2 (en) 2000-09-04 2010-03-09 Mpb Meltec Patent-Und Beteiligungsgesellschaft Mbh Method for identifying cell-specific proteins
US7761239B2 (en) 2003-12-11 2010-07-20 Correlogic Systems, Inc. Method of diagnosing biological states through the use of a centralized, adaptive model, and remote sample processing
US8664358B2 (en) 2007-06-29 2014-03-04 Vermillion, Inc. Predictive markers for ovarian cancer

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MXPA02007665A (en) * 2000-02-08 2004-08-23 Univ Michigan PROTEIN DIFFERENTIAL DEPLOYMENT DESIGNED.
WO2001071360A2 (en) * 2000-03-20 2001-09-27 Eastern Virginia Medical School Prostate cancer markers
MXPA02012167A (en) 2000-06-19 2004-08-19 Correlogic Systems Inc Heuristic method of classification.
SG144731A1 (en) 2000-07-18 2008-08-28 Correlogic Systems Inc A process for discriminating between biological states based on hidden patterns from biological data
US20020132260A1 (en) * 2001-02-22 2002-09-19 Erlander Mark G. Quantitative immunohistochemistry (QIHC)
US6890763B2 (en) * 2001-04-30 2005-05-10 Syn X Pharma, Inc. Biopolymer marker indicative of disease state having a molecular weight of 1350 daltons
US6617308B2 (en) * 2001-04-30 2003-09-09 Syn X Pharma, Inc. Biopolymer marker indicative of disease state having a molecular weight of 1865 daltons
US6602855B2 (en) * 2001-04-30 2003-08-05 Syn X Pharma, Inc. Biopolymer marker indicative of disease state having a molecular weight of 1449 daltons
US20020160423A1 (en) * 2001-04-30 2002-10-31 George Jackowski Biopolymer marker indicative of disease state having a molecular weight of 1536 daltons
US7314717B2 (en) * 2001-04-30 2008-01-01 Nanogen Inc. Biopolymer marker indicative of disease state having a molecular weight of 1562 daltons
US6627608B2 (en) 2001-04-30 2003-09-30 Syn X Pharma, Inc. Biopolymer marker indicative of disease state having a molecular weight of 1206 daltons
US7049397B2 (en) 2001-04-30 2006-05-23 Syn X Pharma, Inc. Biopolymer marker indicative of disease state having a molecular weight of 1211 daltons
US7087435B2 (en) * 2001-04-30 2006-08-08 Syn X Pharma, Inc. Biopolymer marker indicative of disease state having a molecular weight of 2753 daltons
US20020160533A1 (en) * 2001-04-30 2002-10-31 George Jackowski Biopolymer marker indicative of disease state having a molecular of weight of 1525 daltons
US20040198950A1 (en) * 2001-04-30 2004-10-07 George Jackowski Biopolymer marker indicative of disease state having a molecular weight of 1518 daltons
US20020160418A1 (en) * 2001-04-30 2002-10-31 George Jackowski Biopolymer marker indicative of disease state having a molecular weight of 1949 daltons
US20020160532A1 (en) * 2001-04-30 2002-10-31 George Jackowski Biopolymer marker indicative of disease state having a molecular weight of 1998 daltons
US7294688B2 (en) 2001-04-30 2007-11-13 Nanogen Inc. Biopolymer marker indicative of disease state having a molecular weight of 1348 daltons
US6998243B2 (en) 2001-04-30 2006-02-14 Syn X Pharma, Inc. Biopolymer marker indicative of disease state having a molecular weight of 1793 daltons
US20020160421A1 (en) * 2001-04-30 2002-10-31 George Jackowski Method for monitoring and validating stress induction of disease state
US20020160434A1 (en) * 2001-04-30 2002-10-31 George Jackowski Biopolymer marker indicative of disease state having a molecular weight of 1777 daltons
US20030040602A1 (en) * 2001-04-30 2003-02-27 George Jackowski Biopolymer marker indicative of disease state having a molecular weight of 1406 daltons
US6627606B2 (en) * 2001-04-30 2003-09-30 Syn X Pharma, Inc. Biopolymer marker indicative of disease state having a molecular weight of 1465 daltons
US6756476B2 (en) 2001-04-30 2004-06-29 Syn X Pharma, Inc. Biopolymer marker indicative of disease state having a molecular weight of 2021 daltons
US6620787B2 (en) 2001-04-30 2003-09-16 Syn X Pharma, Inc. Biopolymer marker indicative of disease state having a molecular weight of 2267 daltons
US7008800B2 (en) * 2001-04-30 2006-03-07 Artemis Proteomics, Ltd. Biopolymer marker indicative of disease state having a molecular weight of 1077 daltons
US20040224423A1 (en) * 2001-04-30 2004-11-11 George Jackowski Biopolymer marker indicative of disease state having a molecular weight of 2056 daltons
US20020160420A1 (en) * 2001-04-30 2002-10-31 George Jackowski Process for diagnosis of physiological conditions by characterization of proteomic materials
US6599877B2 (en) 2001-04-30 2003-07-29 Syn X Pharma, Inc. Biopolymer marker indicative of disease state having a molecular weight of 1020 daltons
US6703366B2 (en) * 2001-04-30 2004-03-09 George Jackowski Biopolymer marker indicative of disease state having a molecular weight of 1,896 daltons
US20020160417A1 (en) * 2001-04-30 2002-10-31 George Jackowski Biopolymer marker indicative of disease state having a molecular weight of 1424 daltons
US6627607B2 (en) * 2001-04-30 2003-09-30 Syn X Pharma, Inc. Biopolymer marker indicative of disease state having a molecular weight of 1845 daltons
US6677303B2 (en) * 2001-04-30 2004-01-13 Syn X Pharma Biopolymer marker indicative of disease state having a molecular weight of 1097 daltons
US6593298B2 (en) 2001-04-30 2003-07-15 Syn X Pharma, Inc. Biopolymer marker indicative of disease state having a molecular weight of 1690 daltons
US6620786B2 (en) 2001-04-30 2003-09-16 Syn X Pharma, Inc. Biopolymer marker indicative of disease state having molecular weight of 2937 daltons
US6693080B2 (en) * 2001-04-30 2004-02-17 Syn X Pharma Biopolymer marker indicative of disease state having a molecular weight of 1521 daltons
US7314762B2 (en) * 2001-11-21 2008-01-01 Nanogen, Inc. Apolipoprotein biopolymer markers indicative of insulin resistance
US20090093000A1 (en) * 2001-11-23 2009-04-09 George Jackowski Glycoprotein and apolipoprotein biopolymer markers predictive of Alzheimers disease
US20070003974A1 (en) * 2001-11-23 2007-01-04 George Jackowski Cene-E biopolymer marker indicative of age matched control
US7097989B2 (en) * 2001-11-23 2006-08-29 Syn X Pharma, Inc. Complement C3 precursor biopolymer markers predictive of type II diabetes
AU2002336845A1 (en) * 2001-11-23 2003-06-10 Syn.X Pharma, Inc. Globin biopolymer markers indicative of insulin resistance
US7125678B2 (en) * 2001-11-23 2006-10-24 Nanogen, Inc. Protein biopolymer markers predictive of type II diabetes
US20030113808A1 (en) * 2001-12-13 2003-06-19 George Jackowski Apolipoprotein biopolymer markers predictive of alzheimers disease
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US7695752B2 (en) 2003-01-24 2010-04-13 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Target activated microtransfer
US8168568B1 (en) 2003-03-10 2012-05-01 The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services Combinatorial therapy for protein signaling diseases
US20050124965A1 (en) * 2003-12-08 2005-06-09 Becton, Dickinson And Company Phosphatase inhibitor sample collection system
US8962302B2 (en) 2005-11-07 2015-02-24 Canon Kabushiki Kaisha Biological tissue processing substrate for fixing proteins or protein degradation products in tissue for analysis
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CN102621301B (en) * 2011-01-28 2014-10-08 上海铭源数康生物芯片有限公司 Preparation method of nitrocellulose membrane-based protein chip blocking liquid
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CN106644632B (en) * 2016-11-17 2019-08-27 中国科学院苏州生物医学工程技术研究所 Artificial Substrates for Monolayer Cell Tiling
CN113740544A (en) * 2021-07-29 2021-12-03 山东第一医科大学(山东省医学科学院) Method for identifying biomarkers of prolactin protein existing form of human pituitary tissue
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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7075640B2 (en) 1997-10-01 2006-07-11 Arcturus Bioscience, Inc. Consumable for laser capture microdissection
US7221447B2 (en) 1997-10-01 2007-05-22 Molecular Devices Corporation Consumable for laser capture microdissection
US7676328B2 (en) 2000-09-04 2010-03-09 Mpb Meltec Patent-Und Beteiligungsgesellschaft Mbh Method for identifying cell-specific proteins
US7761239B2 (en) 2003-12-11 2010-07-20 Correlogic Systems, Inc. Method of diagnosing biological states through the use of a centralized, adaptive model, and remote sample processing
US8664358B2 (en) 2007-06-29 2014-03-04 Vermillion, Inc. Predictive markers for ovarian cancer
US9274118B2 (en) 2007-06-29 2016-03-01 Vermillion, Inc. Predictive markers for ovarian cancer

Also Published As

Publication number Publication date
WO2000049410A2 (en) 2000-08-24
AU772680B2 (en) 2004-05-06
WO2000049410A9 (en) 2001-08-30
EP1153300A2 (en) 2001-11-14
CA2359649A1 (en) 2000-08-24
AU3367100A (en) 2000-09-04
JP2002537561A (en) 2002-11-05

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