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WO2000049038A3 - Synthetische peptide des regulatorischen virusproteins r (vpr) des humanen immundefizienzvirus typ 1 (hiv-1) und ihre verwendung - Google Patents

Synthetische peptide des regulatorischen virusproteins r (vpr) des humanen immundefizienzvirus typ 1 (hiv-1) und ihre verwendung Download PDF

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Publication number
WO2000049038A3
WO2000049038A3 PCT/DE2000/000525 DE0000525W WO0049038A3 WO 2000049038 A3 WO2000049038 A3 WO 2000049038A3 DE 0000525 W DE0000525 W DE 0000525W WO 0049038 A3 WO0049038 A3 WO 0049038A3
Authority
WO
WIPO (PCT)
Prior art keywords
vpr
hiv
regulatory
human immunodeficiency
protein
Prior art date
Application number
PCT/DE2000/000525
Other languages
English (en)
French (fr)
Other versions
WO2000049038A2 (de
WO2000049038A9 (de
Inventor
Ulrich Schubert
Peter Henklein
Victor Wray
Original Assignee
Ulrich Schubert
Peter Henklein
Victor Wray
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19908752A external-priority patent/DE19908752A1/de
Priority claimed from DE19908766A external-priority patent/DE19908766C2/de
Application filed by Ulrich Schubert, Peter Henklein, Victor Wray filed Critical Ulrich Schubert
Priority to EP00918674A priority Critical patent/EP1155035A2/de
Priority to JP2000599775A priority patent/JP2002540768A/ja
Priority to US09/913,927 priority patent/US6984486B1/en
Publication of WO2000049038A2 publication Critical patent/WO2000049038A2/de
Publication of WO2000049038A3 publication Critical patent/WO2000049038A3/de
Publication of WO2000049038A9 publication Critical patent/WO2000049038A9/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
    • A61K47/6901—Conjugates being cells, cell fragments, viruses, ghosts, red blood cells or viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/18—Antivirals for RNA viruses for HIV
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16311—Human Immunodeficiency Virus, HIV concerning HIV regulatory proteins
    • C12N2740/16322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Molecular Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • AIDS & HIV (AREA)
  • Cell Biology (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Die Erfindung betrifft synthetische (s) Peptide des regulatorischen Virusproteins R (Vpr) des Humanen Immundefizienzvirus Typ 1 (HIV-1), insbesondere die chemische Totalsynthese des 96 Aminosäuren langen Vpr-Proteins (sVpr1-96), eines 47 Aminosäuren langen N-terminalen (sVpr1-47), eines 49 Aminosäuren langen C-terminalen Fragmentes davon (sVpr48-96) sowie der Fragmente sVpr1-20 und sVpr21-40 und weiterer Fragmente mit etwa 15 Aminosäuren. Als HIV-1-regulatorische Proteine finden die Produkte Verwendung in biologischen Assays, in der Analyse der molekularen Struktur und der physikochemischen Eigenschaften von Vpr und dessen Domänen oder zur Erzeugung von Antikörpern gegen Vpr-Peptidsequenzen.
PCT/DE2000/000525 1999-02-19 2000-02-19 Synthetische peptide des regulatorischen virusproteins r (vpr) des humanen immundefizienzvirus typ 1 (hiv-1) und ihre verwendung WO2000049038A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP00918674A EP1155035A2 (de) 1999-02-19 2000-02-19 Synthetische peptide des regulatorischen virusproteins r (vpr) des humanen immundefizienzvirus typ 1 (hiv-1) und ihre verwendung
JP2000599775A JP2002540768A (ja) 1999-02-19 2000-02-19 ヒト免疫不全ウイルス１型（ＨＩＶ−１）ウイルス性調節タンパク質Ｒ（Ｖｐｒ）の合成ペプチドおよびその適用
US09/913,927 US6984486B1 (en) 1999-02-19 2000-02-19 Synthetic peptide of regulatory virus protein R (VPR) of human immunodeficiency virus type 1 (HIV-1) and the utilization thereof

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DE19908766.0 1999-02-19
DE19908752.0 1999-02-19
DE19908752A DE19908752A1 (de) 1999-02-19 1999-02-19 Synthetische Peptide des regulatorischen Virusproteins R (Vpr) des Humanen Immundefizienzvirus Typ 1
DE19908766A DE19908766C2 (de) 1999-02-19 1999-02-19 Verwendung synthetischer Vpr-Peptide des Humanen Immundefizienzvirus Typ 1 (HIV-1) zur Entwicklung von therapeutischen und diagnostischen Reagenzien
CA002356390A CA2356390A1 (en) 1999-02-19 2001-08-17 Synthetic peptide of regulatory virus protein r (vpr) of human immunodeficiency virus type 1 (hiv-1) and the utilization thereof

Publications (3)

Publication Number Publication Date
WO2000049038A2 WO2000049038A2 (de) 2000-08-24
WO2000049038A3 true WO2000049038A3 (de) 2001-03-01
WO2000049038A9 WO2000049038A9 (de) 2001-05-17

Family

ID=27808200

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2000/000525 WO2000049038A2 (de) 1999-02-19 2000-02-19 Synthetische peptide des regulatorischen virusproteins r (vpr) des humanen immundefizienzvirus typ 1 (hiv-1) und ihre verwendung

Country Status (4)

Country Link
EP (1) EP1155035A2 (de)
JP (1) JP2002540768A (de)
CA (1) CA2356390A1 (de)
WO (1) WO2000049038A2 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6664040B2 (en) 2000-05-23 2003-12-16 The Regents Of The University Of California Compositions and methods for delivery of a molecule into a cell
IL149279A0 (en) * 2002-04-22 2002-11-10 Yissum Res Dev Co Anti-nls substances and uses thereof in nuclear import inhibition
FR2860236B1 (fr) * 2003-09-25 2006-01-06 Theraptosis Peptides possedant notamment une activite anti-angiogenique et leurs applications en therapeutique
WO2005103654A2 (fr) * 2004-04-09 2005-11-03 Bioalliance Pharma Methode d’identification de composes actifs sur la replication du virus hiv.
JP2008001598A (ja) * 2004-10-12 2008-01-10 Institute Of Physical & Chemical Research アポトーシス誘導物質を含む医薬組成物
JP4769009B2 (ja) * 2005-04-05 2011-09-07 オリエンタル酵母工業株式会社 Ｖｐｒ特異的モノクローナル抗体を産生するハイブリドーマを作製するためのＶｐｒ抗原、抗Ｖｐｒ特異的モノクローナル抗体産生ハイブリドーマとそのハイブリドーマの産生する抗Ｖｐｒ特異的モノクローナル抗体およびそれを利用したＶｐｒの免疫学的測定
EP2392587B1 (de) * 2006-03-10 2016-05-04 Peptcell Limited Peptidsequenzen und Zusammensetzungen

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995026361A1 (en) * 1994-03-25 1995-10-05 Biomolecular Research Institute Ltd. Vpr AND Vpx PROTEINS OF HIV
WO1998044945A1 (en) * 1997-04-04 1998-10-15 The Immune Response Corporation Non-infectious, protease defective hiv particles and nucleic acid molecules encoding therefor

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5580739A (en) 1986-01-22 1996-12-03 Institut Pasteur Peptides of human immunodeficiency virus type 2 (HIV-2) and in vitro diagnostic methods and kits employing the peptides for the detection of HIV-2
US5780238A (en) 1993-12-15 1998-07-14 The Trustees Of The University Of Pennsylvania VPR receptor protein
US5861161A (en) 1994-09-07 1999-01-19 Universite De Montreal Chimeric proteins comprising a Vpr/Vpx virion incorporation domain for targeting into HIV-1 or HIV-2 virions
US5763190A (en) 1994-09-21 1998-06-09 The Trustees Of The University Of Pennsylvania Methods for the identification of compounds capable of inducing the nuclear translocation of a receptor complex comprising the glucocoticoid receptor type II and viral protein R interacting protein
US5639619A (en) 1994-10-13 1997-06-17 Regents Of The University Of California Screening assay for anti-HIV drugs using the Vpr gene

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995026361A1 (en) * 1994-03-25 1995-10-05 Biomolecular Research Institute Ltd. Vpr AND Vpx PROTEINS OF HIV
WO1998044945A1 (en) * 1997-04-04 1998-10-15 The Immune Response Corporation Non-infectious, protease defective hiv particles and nucleic acid molecules encoding therefor

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 5 February 1999 (1999-02-05), SCHULER W ET AL: "NMR structure of the (52-96) C-terminal domain of the HIV-1 regulatory protein Vpr: Molecular insights into its biological functions.", XP002145860, Database accession no. PREV199900140755 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; December 1999 (1999-12-01), WECKER K ET AL: "NMR structure of the (1-51) N-terminal domain of the HIV-1 regulatory protein Vpr.", XP002145862, Database accession no. PREV200000048307 *
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; November 1999 (1999-11-01), CORNILLE F ET AL: "Efficient solid-phase synthesis of Vpr from HIV-1 using low quantities of uniformly 13C-, 15N-labeled amino acids for NMR structural studies.", XP002145861, Database accession no. PREV200000001193 *
EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 266, no. 2, December 1999 (1999-12-01), pages 359 - 369, ISSN: 0014-2956 *
JOURNAL OF MOLECULAR BIOLOGY, vol. 285, no. 5, 5 February 1999 (1999-02-05), pages 2105 - 2117, ISSN: 0022-2836 *
JOURNAL OF PEPTIDE RESEARCH, vol. 54, no. 5, November 1999 (1999-11-01), pages 427 - 435, ISSN: 1397-002X *
Z. LUO ET AL.: "Structural Studies of Synthetic Peptide Fragments Derived from the HIV-1 Vpr Protein", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 244, no. 3, 27 March 1998 (1998-03-27), ORLANDO, FL US, pages 732 - 736, XP002145859 *

Also Published As

Publication number Publication date
EP1155035A2 (de) 2001-11-21
WO2000049038A2 (de) 2000-08-24
JP2002540768A (ja) 2002-12-03
CA2356390A1 (en) 2003-02-17
WO2000049038A9 (de) 2001-05-17

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