[go: up one dir, main page]

WO2000045839A3 - A method for selecting and producing vaccine and diagnostic preparations - Google Patents

A method for selecting and producing vaccine and diagnostic preparations Download PDF

Info

Publication number
WO2000045839A3
WO2000045839A3 PCT/EP2000/000661 EP0000661W WO0045839A3 WO 2000045839 A3 WO2000045839 A3 WO 2000045839A3 EP 0000661 W EP0000661 W EP 0000661W WO 0045839 A3 WO0045839 A3 WO 0045839A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene library
antibody
preparation
selecting
pathogenic organism
Prior art date
Application number
PCT/EP2000/000661
Other languages
French (fr)
Other versions
WO2000045839A2 (en
Inventor
Gabain Alexander Von
Michael Buschle
Original Assignee
Cistem Biotechnologies Gmbh
Gabain Alexander Von
Michael Buschle
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cistem Biotechnologies Gmbh, Gabain Alexander Von, Michael Buschle filed Critical Cistem Biotechnologies Gmbh
Priority to AU32774/00A priority Critical patent/AU3277400A/en
Priority to EP00910621A priority patent/EP1144002A3/en
Publication of WO2000045839A2 publication Critical patent/WO2000045839A2/en
Publication of WO2000045839A3 publication Critical patent/WO2000045839A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)

Abstract

There is disclosed a method for selecting and producing vaccine and diagnostic preparations against a specific pathogenic organism, wherein a non-human vertebrate is immunized with a preparation of the pathogenic organism, samples containing the antibodies to the pathogen-preparation are taken from immunized vertebrates, a gene library of the genome of the pathogenic organism is produced, which gene library is capable of being expressed in host cells, the pathogen polypeptide sequences-expessing gene library-host cells being selectable as regards their binding to an antibody, antigenic polypeptides are identified by contacting the gene library with the antibody-containing sample taken, and those clones of the gene library are selected whose expressed polypeptides bind to an antibody, and the identified antigenic polypeptides are finished to a vaccine or diagnostic preparation.
PCT/EP2000/000661 1999-02-01 2000-01-28 A method for selecting and producing vaccine and diagnostic preparations WO2000045839A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU32774/00A AU3277400A (en) 1999-02-01 2000-01-28 A method for selecting and producing vaccine and diagnostic preparations
EP00910621A EP1144002A3 (en) 1999-02-01 2000-01-28 A method for selecting and producing vaccine and diagnostic preparations

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AT0012999A AT407643B (en) 1999-02-01 1999-02-01 METHOD FOR SELECTION AND PRODUCTION OF VACCINE AND DIAGNOSTIC PREPARATIONS
ATA129/99 1999-02-01

Publications (2)

Publication Number Publication Date
WO2000045839A2 WO2000045839A2 (en) 2000-08-10
WO2000045839A3 true WO2000045839A3 (en) 2001-10-04

Family

ID=3482303

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/000661 WO2000045839A2 (en) 1999-02-01 2000-01-28 A method for selecting and producing vaccine and diagnostic preparations

Country Status (4)

Country Link
EP (1) EP1144002A3 (en)
AT (1) AT407643B (en)
AU (1) AU3277400A (en)
WO (1) WO2000045839A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT410798B (en) * 2001-01-26 2003-07-25 Cistem Biotechnologies Gmbh METHOD FOR IDENTIFYING, ISOLATING AND PRODUCING ANTIGENS AGAINST A SPECIFIC PATHOGEN
AU2003254555A1 (en) * 2002-07-26 2004-02-23 Intercell Ag S. aureus antigene
US20060193866A1 (en) * 2003-04-22 2006-08-31 Intercell Ag H pylori antigens

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4906564A (en) * 1987-03-13 1990-03-06 The United States Of America As Represented By The Secretary Of The Army Antigenic determinants recognized by antibodies obtained using a pathogenic agent or a derivative thereof that presents a restricted set of antigens
WO1999030151A1 (en) * 1997-12-05 1999-06-17 Intercell Biomedizinische Forschungs- Und Entwicklungs Gmbh Method and cells to screen combinatorial libraries

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4906564A (en) * 1987-03-13 1990-03-06 The United States Of America As Represented By The Secretary Of The Army Antigenic determinants recognized by antibodies obtained using a pathogenic agent or a derivative thereof that presents a restricted set of antigens
WO1999030151A1 (en) * 1997-12-05 1999-06-17 Intercell Biomedizinische Forschungs- Und Entwicklungs Gmbh Method and cells to screen combinatorial libraries

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
A. MUSTAFA ET AL.: "Human T cell clones recognize a major M. leprae protein antigen expressed in E. coli", NATURE, vol. 319, 1986, pages 63 - 66, XP002148356 *
C. GENTRY-WEEKS ET AL.: "Cloning and sequencing a gene encoding a 21kda outer membrane protein from B. avium and expression of the gene in S. typhimurium", J. BACTERIOL., vol. 174, no. 23, 1992, pages 7729 - 7742, XP000946384 *
D. PAWALEC ET AL.: "Cloning and characterisation of a C. jejuni 72DZ92 gene encoding 30kda immunopositive protein component o the ABC transport system; expression of the gene in avirulent S. typhimurium", FEMS IMMUNOL. MED. MICROBIOL., vol. 19, 1997, pages 137 - 150, XP000946387 *
P. TOYE ET AL.: "An assay for the identification of antigens recognized by cytotoxic T cells based on transient transfection of COS cells", J. IMMUNOL. METHODS, vol. 187, 1995, pages 95 - 101, XP000946345 *
P.ROMERO ET AL.: "Novel methods to monitor antigen-specific cytotoxic T cell responses in cancer immunotherapy", MOL. MED. TODAY, vol. 4, 1998, pages 305 - 312, XP000946379 *

Also Published As

Publication number Publication date
EP1144002A3 (en) 2001-11-28
WO2000045839A2 (en) 2000-08-10
AT407643B (en) 2001-05-25
EP1144002A2 (en) 2001-10-17
AU3277400A (en) 2000-08-25
ATA12999A (en) 2000-09-15

Similar Documents

Publication Publication Date Title
Nilsson et al. Towards a human proteome atlas: high‐throughput generation of mono‐specific antibodies for tissue profiling
JP6951417B2 (en) FC-binding proteins for affinity chromatography, as well as affinity isolation matrices containing Fc-binding proteins and their use
WO2002020569A3 (en) Mammalian genes; related reagents and methods
WO2002084252A3 (en) Epitope-captured antibody display
WO2015067768A1 (en) High-affinity monoclonal anti-strep-tag antibody
WO2000056901A3 (en) Linear and circular expression elements
CN113061184B (en) anti-CK 7 protein monoclonal antibody, cell strain thereof, preparation method and application
KR890701761A (en) Method of Preparation of Immune Diagnostic Reagent
WO2005010023A3 (en) Method for prediction of an epitope
CN114702578B (en) Novel coronavirus Omicron mutant strain specific antibody and application thereof
JP7245373B2 (en) Method for controlling affinity of antibody for antigen, antibody with altered affinity for antigen, and method for producing the same
WO2002035237A3 (en) Method for in vivo identification of intracellular epitopes
WO2000045839A3 (en) A method for selecting and producing vaccine and diagnostic preparations
CN106834319A (en) Recombinant protein coding sequence, recombinant protein and preparation method of monoclonal antibody of recombinant protein
CN119306838A (en) A shark nanobody against human PD-1 and its application
CN115947854B (en) Anti-human CD40 protein monoclonal antibody, preparation method and application thereof
Casey et al. Peptides specific for Mycobacterium avium subspecies paratuberculosis infection: diagnostic potential
CN113831410B (en) anti-CD 56 protein monoclonal antibody and cell strain, preparation method and application thereof
WO2005065711A3 (en) Mammalian receptor proteins; related reagents and methods
Wu et al. Preparation of monoclonal antibody bank against whole water‐soluble proteins from rapid‐growing bamboo shoots
CN113845592A (en) anti-CK 5/6 protein monoclonal antibody, cell strain thereof, preparation method and application
CA2488127A1 (en) Antibodies directed against prothrombin fragment f1+2, the preparation and use thereof
WO2003091700A3 (en) Epitope mapping using nuclear magnetic resonance
CN106928358A (en) A kind of CD105 nano antibodies Nb168
CN101074263B (en) Recombinant proteinA monoclonal antibody, its production and use

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2000910621

Country of ref document: EP

AK Designated states

Kind code of ref document: A3

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWP Wipo information: published in national office

Ref document number: 2000910621

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWW Wipo information: withdrawn in national office

Ref document number: 2000910621

Country of ref document: EP