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WO2000010675A1 - Segmented chromatography column - Google Patents

Segmented chromatography column Download PDF

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Publication number
WO2000010675A1
WO2000010675A1 PCT/US1999/016009 US9916009W WO0010675A1 WO 2000010675 A1 WO2000010675 A1 WO 2000010675A1 US 9916009 W US9916009 W US 9916009W WO 0010675 A1 WO0010675 A1 WO 0010675A1
Authority
WO
WIPO (PCT)
Prior art keywords
tube
media
porous member
media bed
bounded
Prior art date
Application number
PCT/US1999/016009
Other languages
French (fr)
Inventor
Peter C. Vandavelaar
Original Assignee
Dyax Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dyax Corporation filed Critical Dyax Corporation
Priority to AU52139/99A priority Critical patent/AU5213999A/en
Publication of WO2000010675A1 publication Critical patent/WO2000010675A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6052—Construction of the column body
    • G01N30/6069—Construction of the column body with compartments or bed substructure
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6004—Construction of the column end pieces
    • G01N30/6017—Fluid distributors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6091—Cartridges
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
    • G01N30/463—Flow patterns using more than one column with serial coupling of separation columns for multidimensional chromatography

Definitions

  • the invention relates to an apparatus for liquid chromatography analyses and a method for making the same .
  • Liquid chromatography is a technique for separating the individual compounds that exist in a subject sample.
  • the subject sample is carried in a liquid, called a mobile phase.
  • the mobile phase carrying the subject sample is caused to migrate through a media, called a stationary phase.
  • a media called a stationary phase.
  • Different compounds will have differing rates of migration through the media, which effects the separation of the components in the subject sample.
  • the chromatography media is contained within a tubular column, which may be either reusable or disposable, such as disposable cartridges, which are pre-loaded with media.
  • the media bed is bounded axially by porous plates, or plates containing defined flow paths, through which the mobile phase will flow. (See U.S. Pat. No. 4,250,035 to McDonald et al . )
  • chromatography columns depend upon the degree of compaction of the media bed.
  • plate count refers to the media's capacity to separate compounds. More compact media beds have more plates, per unit of column length, than less compact beds. Longer columns tend to have a higher overall plate count than shorter columns, for a given diameter. However, shorter columns tend to be easier to pack and thus have more plates per unit of column length than longer columns .
  • symmetry indicates the consistency of fluid flow through the media bed. If fluid flow throughout the media bed is uniform, the concentration of a compound exiting the column will follow a Gaussian curve, when plotted as a function of time. Such a Gaussian curve is usually desirable in chromatography analyses. Symmetry is greatest in columns whose media beds have a uniform degree of compaction throughout their length. As with plate count, column length typically detracts from uniformity, A uniform degree of compaction is more difficult to achieve in longer columns, as opposed to shorter ones .
  • bed stability refers to the durability of a column, that is, the amount of handling it can withstand before performance is affected adversely.
  • longer columns are less durable than shorter ones, because longer columns create more opportunities for media to shift within the column, so as to alter the uniformity of compaction throughout the bed.
  • the invention features, in general, a chromatography column in which the media is divided into a plurality of segments separated and bounded by porous plates .
  • the apparatus has a tube within which upper, intermediate, and lower porous plates are press-fitted.
  • a first media bed is packed between said upper porous plate and said intermediate porous plate.
  • a second media bed is packed between said intermediate porous plate and said lower porous plate.
  • the invention also includes methods for making the apparatus.
  • a porous plate is press-fitted within a tube.
  • a media bed is then deposited within the tube and packed to a desired level of compaction.
  • An intermediate porous plate is press- fitted within the tube, so that the media bed is bounded between the lower porous plate and the intermediate porous plate.
  • the further media bed is then deposited within the tube and packed to a desired degree of compaction.
  • a further porous member is then press-fitted into the tube, so that the last-mentioned media bed is bounded by the intermediate porous plate and the upper porous plate.
  • Embodiments of the invention may include one or more of the following advantages.
  • Media bed segments within a particular column have a relatively uniform degree of compaction.
  • Media bed segments may be packed individually, in order to achieve a high degree of compaction.
  • Media beds in columns according to the invention can be packed with less difficulty than media beds non-segmented columns having approximately the same dimensions.
  • Chromatography columns according to the invention have improved symmetry, plate count, and/or bed stability.
  • the invention allows longer chromatography columns to achieve many of the superior performance characteristics of shorter columns.
  • One long media bed may be subdivided into two or more shorter beds .
  • FIG. 1 is a diagrammatic vertical-sectional view of an apparatus according to the invention.
  • FIG. 2 is a diagrammatic vertical-sectional view of an alternate embodiment of an apparatus according to the invention.
  • FIG. 3 is a diagrammatic vertical-sectional view of an alternate embodiment of an apparatus according to the invention.
  • chromatography apparatus 10 has longitudinal axis 11 and includes tubular column 12 containing upper porous plate 14 lower porous plate 16, and intermediate porous plate 20, first media bed 18, and second media bed 22.
  • First media bed 18 is bounded axially by upper porous plate 14 and intermediate porous plate 20.
  • Second media bed 22 is bounded axially by intermediate porous plate 20 and lower porous plate 16.
  • chromatography apparatus 110 has longitudinal axis 111 and includes tubular column 112 containing upper porous plate 114 lower porous plate 116, first intermediate porous plate 120, second intermediate porous plate 124, first media bed 118, second media bed 122, and third media bed 126.
  • First media bed 118 is bounded axially by upper porous plate 114 and first intermediate porous plate 120.
  • Second media bed 122 is bounded axially by first intermediate porous plate 120 and second intermediate porous plate 124.
  • Third media bed 126 is bounded axially by second intermediate porous plate 124 and lower porous plate 116.
  • chromatography apparatus 210 has longitudinal axis 211 and includes tubular column 212 containing upper porous plate 214 lower porous plate 216, and intermediate porous plate 220, first media bed 218, and second media bed 222.
  • First media bed 218 is bounded axially by upper porous plate 214 and intermediate porous plate 220.
  • Second media bed 222 is bounded axially by intermediate porous plate 220 and lower porous plate 216.
  • the media beds in embodiments of the invention are well-packed, so as to improve the plate count, symmetry, and bed stability of the columns.
  • the media beds in an apparatus according to the invention preferably have approximately the same length.
  • media beds 18 and 22 have approximately the same length.
  • media beds 118, 122, and 126 have approximately the same length.
  • individual beds in a particular apparatus may have any desired lengths.
  • first media bed 218 and second media bed 222 have dissimilar lengths.
  • the FIG. 3 embodiment is identical to the FIG. 1 embodiment.
  • the media bed segments within a particular column have a relatively uniform, high degree of compaction.
  • the chromatography columns have improved symmetry, plate count, and bed stability.
  • Apparatuses according to the invention can be manufactured in a number of ways .
  • lower porous plate 116 is press-fitted into tube 112, which is then filled with the other elements.
  • third media bed 126 is deposited within tube 112 so that third media bed 126 is bounded by lower porous plate 116.
  • Third media bed 126 is then packed to provide the desired degree of compaction.
  • Second intermediate porous plate 124 is then press-fitted within tube 112, so that second intermediate porous plate 124 bounds third media bed 126. Fitting second intermediate porous plate 124 into tube 112 may further compact third media bed 126. Second media bed 122 is then deposited within tube 112, so that it is bound by second intermediate porous plate 124. Second media bed 122 is then packed to give it the desired amount of compaction.
  • First intermediate porous plate 120 is then press- fitted into tube 112 so that first intermediate porous plate 120 bounds second media bed 122. Fitting first intermediate porous plate 120 into tube 112 may further compact second media bed 122. First media bed 118 is then deposited within tube 112 so that it is bound by first intermediate porous plate 120. First media bed 118 is then packed to give it the desired amount of compaction.
  • upper porous plate 114 is press-fitted within tube 112, so that upper porous plate 114 bounds first media bed 118. Fitting upper porous plate 114 into tube 112 may further compact first media bed 118.
  • an intermediate porous plate is inserted into the tube, in the first step.
  • the media beds and porous plates are added on both sides of the first intermediate porous plate inserted into the tube.
  • the various media beds in a segmented column may contain different types of media; thus, a subject sample may be analyzed by a plurality of different media in one column.
  • Columns according to the invention may be used with pressurizable containment vessels for laterally compressing the media.
  • the invention is applicable to any type of column, including disposable pre-packed cartridges, columns with either flexible or rigid walls, and columns made of, e.g., glass, metal, or a synthetic material.
  • the invention may employ any type of porous members, such as porous plates or frits, or plates with flow paths machined or molded into them.

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

A chromatography column (12) including a plurality of media beds (18, 22) separated and bounded by a plurality of porous members (14, 16, 20).

Description

SEGMENTED CHROMATOGRAPHY COLUMN
Background of the Invention The invention relates to an apparatus for liquid chromatography analyses and a method for making the same .
Liquid chromatography is a technique for separating the individual compounds that exist in a subject sample. In employing the technique, the subject sample is carried in a liquid, called a mobile phase.
The mobile phase carrying the subject sample is caused to migrate through a media, called a stationary phase. Different compounds will have differing rates of migration through the media, which effects the separation of the components in the subject sample. The chromatography media is contained within a tubular column, which may be either reusable or disposable, such as disposable cartridges, which are pre-loaded with media. The media bed is bounded axially by porous plates, or plates containing defined flow paths, through which the mobile phase will flow. (See U.S. Pat. No. 4,250,035 to McDonald et al . )
Several characteristics of chromatography columns depend upon the degree of compaction of the media bed. One of these characteristics is "plate count," which refers to the media's capacity to separate compounds. More compact media beds have more plates, per unit of column length, than less compact beds. Longer columns tend to have a higher overall plate count than shorter columns, for a given diameter. However, shorter columns tend to be easier to pack and thus have more plates per unit of column length than longer columns .
Another characteristic of column performance is "symmetry, " which indicates the consistency of fluid flow through the media bed. If fluid flow throughout the media bed is uniform, the concentration of a compound exiting the column will follow a Gaussian curve, when plotted as a function of time. Such a Gaussian curve is usually desirable in chromatography analyses. Symmetry is greatest in columns whose media beds have a uniform degree of compaction throughout their length. As with plate count, column length typically detracts from uniformity, A uniform degree of compaction is more difficult to achieve in longer columns, as opposed to shorter ones .
Finally, "bed stability" refers to the durability of a column, that is, the amount of handling it can withstand before performance is affected adversely. Typically, longer columns are less durable than shorter ones, because longer columns create more opportunities for media to shift within the column, so as to alter the uniformity of compaction throughout the bed.
Summary of the Invention The invention features, in general, a chromatography column in which the media is divided into a plurality of segments separated and bounded by porous plates .
In preferred embodiments, the apparatus has a tube within which upper, intermediate, and lower porous plates are press-fitted. A first media bed is packed between said upper porous plate and said intermediate porous plate. A second media bed is packed between said intermediate porous plate and said lower porous plate. The invention also includes methods for making the apparatus. In a preferred method, a porous plate is press-fitted within a tube. A media bed is then deposited within the tube and packed to a desired level of compaction. An intermediate porous plate is press- fitted within the tube, so that the media bed is bounded between the lower porous plate and the intermediate porous plate. The further media bed is then deposited within the tube and packed to a desired degree of compaction. A further porous member is then press-fitted into the tube, so that the last-mentioned media bed is bounded by the intermediate porous plate and the upper porous plate.
Embodiments of the invention may include one or more of the following advantages. Media bed segments within a particular column have a relatively uniform degree of compaction. Media bed segments may be packed individually, in order to achieve a high degree of compaction. Media beds in columns according to the invention can be packed with less difficulty than media beds non-segmented columns having approximately the same dimensions. Chromatography columns according to the invention have improved symmetry, plate count, and/or bed stability. The invention allows longer chromatography columns to achieve many of the superior performance characteristics of shorter columns. One long media bed may be subdivided into two or more shorter beds . Other features and advantages of the invention will be apparent from the following description of the preferred embodiments thereof and from the claims. Brief Description of the Drawings
FIG. 1 is a diagrammatic vertical-sectional view of an apparatus according to the invention.
FIG. 2 is a diagrammatic vertical-sectional view of an alternate embodiment of an apparatus according to the invention.
FIG. 3 is a diagrammatic vertical-sectional view of an alternate embodiment of an apparatus according to the invention.
Description of the Preferred Embodiments Referring to FIG. 1, chromatography apparatus 10 has longitudinal axis 11 and includes tubular column 12 containing upper porous plate 14 lower porous plate 16, and intermediate porous plate 20, first media bed 18, and second media bed 22.
First media bed 18 is bounded axially by upper porous plate 14 and intermediate porous plate 20. Second media bed 22 is bounded axially by intermediate porous plate 20 and lower porous plate 16. Referring to FIG. 2, in an alternate preferred embodiment, chromatography apparatus 110 has longitudinal axis 111 and includes tubular column 112 containing upper porous plate 114 lower porous plate 116, first intermediate porous plate 120, second intermediate porous plate 124, first media bed 118, second media bed 122, and third media bed 126.
First media bed 118 is bounded axially by upper porous plate 114 and first intermediate porous plate 120. Second media bed 122 is bounded axially by first intermediate porous plate 120 and second intermediate porous plate 124. Third media bed 126 is bounded axially by second intermediate porous plate 124 and lower porous plate 116.
Referring to FIG. 3, in another alternate preferred embodiment, chromatography apparatus 210 has longitudinal axis 211 and includes tubular column 212 containing upper porous plate 214 lower porous plate 216, and intermediate porous plate 220, first media bed 218, and second media bed 222. First media bed 218 is bounded axially by upper porous plate 214 and intermediate porous plate 220. Second media bed 222 is bounded axially by intermediate porous plate 220 and lower porous plate 216. Preferably, the media beds in embodiments of the invention are well-packed, so as to improve the plate count, symmetry, and bed stability of the columns.
The media beds in an apparatus according to the invention preferably have approximately the same length. Referring to FIG. 1, media beds 18 and 22 have approximately the same length. Referring to FIG. 2, media beds 118, 122, and 126 have approximately the same length. However, individual beds in a particular apparatus may have any desired lengths. For example, in the alternate preferred embodiment of FIG. 3, first media bed 218 and second media bed 222 have dissimilar lengths. In other respects, the FIG. 3 embodiment is identical to the FIG. 1 embodiment.
The media bed segments within a particular column have a relatively uniform, high degree of compaction. The chromatography columns have improved symmetry, plate count, and bed stability. Apparatuses according to the invention can be manufactured in a number of ways . In a preferred method of making, for example, apparatus 110 of FIG. 2, lower porous plate 116 is press-fitted into tube 112, which is then filled with the other elements. After lower porous plate 116 has been press-fitted within tube 112, third media bed 126 is deposited within tube 112 so that third media bed 126 is bounded by lower porous plate 116. Third media bed 126 is then packed to provide the desired degree of compaction. Second intermediate porous plate 124 is then press-fitted within tube 112, so that second intermediate porous plate 124 bounds third media bed 126. Fitting second intermediate porous plate 124 into tube 112 may further compact third media bed 126. Second media bed 122 is then deposited within tube 112, so that it is bound by second intermediate porous plate 124. Second media bed 122 is then packed to give it the desired amount of compaction.
First intermediate porous plate 120 is then press- fitted into tube 112 so that first intermediate porous plate 120 bounds second media bed 122. Fitting first intermediate porous plate 120 into tube 112 may further compact second media bed 122. First media bed 118 is then deposited within tube 112 so that it is bound by first intermediate porous plate 120. First media bed 118 is then packed to give it the desired amount of compaction.
Finally, upper porous plate 114 is press-fitted within tube 112, so that upper porous plate 114 bounds first media bed 118. Fitting upper porous plate 114 into tube 112 may further compact first media bed 118.
In alternate methods of manufacturing apparatuses according to the invention, an intermediate porous plate is inserted into the tube, in the first step. The media beds and porous plates are added on both sides of the first intermediate porous plate inserted into the tube.
In alternate methods of manufacture, compaction of the various media beds takes place after all the media beds and porous plates have been fitted within the tube. Other embodiments of the invention are within the scope of the claims. The various media beds in a segmented column may contain different types of media; thus, a subject sample may be analyzed by a plurality of different media in one column. Columns according to the invention may be used with pressurizable containment vessels for laterally compressing the media. The invention is applicable to any type of column, including disposable pre-packed cartridges, columns with either flexible or rigid walls, and columns made of, e.g., glass, metal, or a synthetic material. The invention may employ any type of porous members, such as porous plates or frits, or plates with flow paths machined or molded into them.
What is claimed is:

Claims

1. An apparatus for performing liquid chromatography analyses comprising: a straight tube, having an axis; a chromatography media contained inside said tube, said media having a uniform composition throughout said tube ; at least three porous members being spaced from each other so as to divide said media into segments, each of said segments being bounded axially by two of said porous members.
2. The apparatus of claim 1 wherein there are three of said porous members .
3. The apparatus of claim 1 wherein said segments have approximately equal lengths .
4. The apparatus of claim 1 wherein said media segments are packed.
5. The apparatus of claim 1 wherein said tube has flexible walls.
6. The apparatus of claim 1 wherein said tube has rigid walls.
7. An apparatus . for performing chromatography analyses comprising: a tube ; a plurality of porous members disposed within said tube, said plurality of porous members including at least first, second, and third porous members; a plurality of media beds within said tube, including first and second media beds, said first media bed bounded between said first porous member and said second porous member, said second media bed bounded between said second porous member and said third porous member .
8. The apparatus of claim 7 wherein said plurality of porous members includes a fourth porous member and said plurality of media beds includes a third media bed, said third media bed bounded between said third porous member and said fourth porous member.
9. The apparatus of claim 7 wherein said media beds are packed.
10. The apparatus of claim 7 wherein said tube has flexible walls.
11. The apparatus of claim 7 wherein said tube has rigid walls.
12. A method for making an apparatus for chromatography analyses comprising: providing a tube; fitting a first porous member into said tube; depositing a first media bed into said tube; fitting a second porous member into said tube, so that said first media bed is bounded by said first porous member and said second porous member; depositing a second media bed into said tube; and fitting a third porous member into said tube, so that said second media bed is bounded by said second porous member and said third porous member.
13. The method of claim 12, further comprising the steps of : depositing a third media bed into said tube; and fitting a fourth porous member into said tube, so that said third media bed is bounded by said third porous member and said fourth porous member.
14. The method of claim 13 wherein said depositing steps include compacting said first, second, and third media beds.
15. A method for making an apparatus for chromatography analyses comprising: providing a tube having a first end and a second end; fitting a first porous member inside said tube; depositing a first media bed into said first end; fitting a second porous member into said tube, so that said first media bed is bounded by said first porous member and said second porous member; depositing a second media bed into said second end; and fitting a third porous member into said tube, so that said second media bed is bounded by said first porous member and said third porous member.
PCT/US1999/016009 1998-08-20 1999-07-14 Segmented chromatography column WO2000010675A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU52139/99A AU5213999A (en) 1998-08-20 1999-07-14 Segmented chromatography column

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13737498A 1998-08-20 1998-08-20
US09/137,374 1998-08-20

Publications (1)

Publication Number Publication Date
WO2000010675A1 true WO2000010675A1 (en) 2000-03-02

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007531866A (en) * 2003-07-14 2007-11-08 ウオーターズ・インベストメンツ・リミテツド Separator with integrated guard column
EP1916522A1 (en) * 2006-10-25 2008-04-30 Agilent Technologies, Inc. Column having separated sections of stationary phase
US20100012588A1 (en) * 2006-08-28 2010-01-21 Maciej Siewinski system and method for the extra-corporeal purification of blood of pathogenic enzymes
WO2011012296A1 (en) 2009-07-30 2011-02-03 F. Hoffmann-La Roche Ag Moveable chromatography column separator
US7887754B2 (en) 2003-02-07 2011-02-15 Waters Technologies Corporation Polymeric solid supports for chromatography nanocolumns
US7938961B2 (en) * 2003-02-04 2011-05-10 Waters Technologies Corporation Capillary loop with a built-in retaining frit
WO2015082872A1 (en) * 2013-12-04 2015-06-11 Johnson Matthey Public Limited Company Scavenging unit and method using the same
US11241638B2 (en) 2011-02-02 2022-02-08 Hoffmann-La Roche Inc. Chromatography column support

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4719011A (en) * 1985-03-22 1988-01-12 H. T. Chemicals, Inc. High pressure liquid chromatography columns

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4719011A (en) * 1985-03-22 1988-01-12 H. T. Chemicals, Inc. High pressure liquid chromatography columns

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SNYDER: "INTRODUCTION TO MODERN LIQUID CHROMATOGRAPY, PASSAGE", INTRODUCTION TO MODERN LIQUID CHROMATOGRAPHY, XX, XX, 1 January 1979 (1979-01-01), XX, pages 228/229, XP002924917 *

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7938961B2 (en) * 2003-02-04 2011-05-10 Waters Technologies Corporation Capillary loop with a built-in retaining frit
US7887754B2 (en) 2003-02-07 2011-02-15 Waters Technologies Corporation Polymeric solid supports for chromatography nanocolumns
GB2418627B (en) * 2003-07-14 2008-02-20 Waters Investments Ltd Separation device with integral guard column
US8685239B2 (en) 2003-07-14 2014-04-01 Waters Technologies Corporation Separation device with integral guard column
JP2007531866A (en) * 2003-07-14 2007-11-08 ウオーターズ・インベストメンツ・リミテツド Separator with integrated guard column
JP4931584B2 (en) * 2003-07-14 2012-05-16 ウオーターズ・テクノロジーズ・コーポレイシヨン Separator with integrated guard column
US20100012588A1 (en) * 2006-08-28 2010-01-21 Maciej Siewinski system and method for the extra-corporeal purification of blood of pathogenic enzymes
EP1916522A1 (en) * 2006-10-25 2008-04-30 Agilent Technologies, Inc. Column having separated sections of stationary phase
WO2011012296A1 (en) 2009-07-30 2011-02-03 F. Hoffmann-La Roche Ag Moveable chromatography column separator
AU2010278289B2 (en) * 2009-07-30 2016-01-21 F. Hoffmann-La Roche Ag Moveable chromatography column separator
US9289699B2 (en) 2009-07-30 2016-03-22 Hoffmann-La Roche Inc. Moveable chromatography column separator
US10052567B2 (en) 2009-07-30 2018-08-21 Hoffmann-La Roche Inc. Moveable chromatography column separator
US11241638B2 (en) 2011-02-02 2022-02-08 Hoffmann-La Roche Inc. Chromatography column support
WO2015082872A1 (en) * 2013-12-04 2015-06-11 Johnson Matthey Public Limited Company Scavenging unit and method using the same

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