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WO1999055841A2 - Procede d'obtention de cellules souches - Google Patents

Procede d'obtention de cellules souches Download PDF

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Publication number
WO1999055841A2
WO1999055841A2 PCT/GB1999/001333 GB9901333W WO9955841A2 WO 1999055841 A2 WO1999055841 A2 WO 1999055841A2 GB 9901333 W GB9901333 W GB 9901333W WO 9955841 A2 WO9955841 A2 WO 9955841A2
Authority
WO
WIPO (PCT)
Prior art keywords
cell
cells
marker
nucleus
oocyte
Prior art date
Application number
PCT/GB1999/001333
Other languages
English (en)
Other versions
WO1999055841A3 (fr
Inventor
Austin Smith
Peter Scott Mountford
Megan Munsie
Original Assignee
University Of Edinburgh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University Of Edinburgh filed Critical University Of Edinburgh
Priority to IL13932299A priority Critical patent/IL139322A0/xx
Priority to AU36212/99A priority patent/AU3621299A/en
Priority to JP2000545985A priority patent/JP2002512787A/ja
Priority to CA002329975A priority patent/CA2329975A1/fr
Priority to EP99918186A priority patent/EP1073719A2/fr
Publication of WO1999055841A2 publication Critical patent/WO1999055841A2/fr
Publication of WO1999055841A3 publication Critical patent/WO1999055841A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/04—Cells produced using nuclear transfer

Definitions

  • Embryonic development in mammals commences with a series of cleavage divisions that generates equivalent totipotent cells. Maternal factors deposited in the oocyte dictate a characteristic number and timing of cleavages for each species. Activation of the zygotic genome occurs during the cleavage process. This is followed by the divergence of two distinct cell lineages, an outer epithelial cell layer, the trophectoderm, and an internal cluster of cells, the inner cell mass (ICM) . The trophectoderm subsequently contributes the embryo- derived component of the placenta, whereas the ICM is the founder of the embryo proper.
  • ICM inner cell mass
  • the present invention provides a combination of transplantation of a nucleus from a somatic cell into an enucleated oocyte with identification of daughter cells of the oocyte that express a gene characteristic of a desired stem cell .
  • somatic cell of the predetermined species which cell contains a gene coding for a marker which is differentially expressed in (a) desired stem cells, and (b) cells other than desired stem cells;
  • a selection/reporter gene, ?geo is integrated by homologous recombination into the sox2 gene, which is expressed uniformly in precursor cells in the neural plate and neural tube.
  • a transgene containing this construct is introduced into somatic cells, and application of G41 8 after nuclear transplantation results in the efficient isolation of viable ?geo-positive cells.
  • These cells express markers of neuroepithelial precursor cells. They can be propagated and will differentiate efficiently into networks of neuron-like cells that express a variety of neuronal markers.
  • an in vitro system for genetic and molecular dissection of mammalian neural differentiation is provided by the invention, and also a route for the production of pure populations of normal or genetically modified neural precursors and neurons for functional studies including transplantation.
  • the technology of the present invention is of application to all mammalian cell types, and to obtaining embryonic stem cells of a species for which there is a low (or no) availability of recipient oocytes. It is preferred that the somatic cell is a human cell, and surprisingly the invention provides for creation of human embryonic stem cells from a non-human oocyte - pig or cow oocytes are used in the recited examples below, as they are readily available.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé permettant d'obtenir une cellule souche. Ce procédé consiste à obtenir une cellule somatique contenant un gène codant pour un marqueur qui est exprimé de manière différentielle dans (a) les cellules souches requises et (b) les cellules autres que les cellules souches requises. Ce procédé consiste ensuite à obtenir un ovocyte énucléé, à transférer le noyau de la cellule somatique dans l'ovocyte énucléé pour former un ovocyte transgénique puis à cultiver ce dernier ovocyte pour produire une culture de cellules dérivées de l'ovocyte transgénique. Ce procédé consiste enfin à identifier une cellule de la culture exprimant le marqueur, ce qui indique alors que le noyau de cette cellule a été reprogrammé. L'invention traite également de dosages pour des facteurs qui peuvent reprogrammer les noyaux et les cellules souches obtenus directement ou indirectement à l'aide des procédés de reprogrammation nucléaires selon l'invention
PCT/GB1999/001333 1998-04-29 1999-04-29 Procede d'obtention de cellules souches WO1999055841A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
IL13932299A IL139322A0 (en) 1998-04-29 1999-04-29 Process for obtaining stem cells
AU36212/99A AU3621299A (en) 1998-04-29 1999-04-29 Process for obtaining stem cells
JP2000545985A JP2002512787A (ja) 1998-04-29 1999-04-29 幹細胞を得る方法
CA002329975A CA2329975A1 (fr) 1998-04-29 1999-04-29 Procede d'obtention de cellules souches
EP99918186A EP1073719A2 (fr) 1998-04-29 1999-04-29 Procede d'obtention de cellules souches

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9809178.8 1998-04-29
GBGB9809178.8A GB9809178D0 (en) 1998-04-29 1998-04-29 Nuclear reprogramming of somatic cells

Publications (2)

Publication Number Publication Date
WO1999055841A2 true WO1999055841A2 (fr) 1999-11-04
WO1999055841A3 WO1999055841A3 (fr) 2000-01-06

Family

ID=10831203

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1999/001333 WO1999055841A2 (fr) 1998-04-29 1999-04-29 Procede d'obtention de cellules souches

Country Status (8)

Country Link
EP (1) EP1073719A2 (fr)
JP (1) JP2002512787A (fr)
AU (1) AU3621299A (fr)
CA (1) CA2329975A1 (fr)
GB (1) GB9809178D0 (fr)
IL (1) IL139322A0 (fr)
MX (1) MXPA00010473A (fr)
WO (1) WO1999055841A2 (fr)

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1214403A1 (fr) * 1999-09-07 2002-06-19 Advanced Cell Technology, Inc. Procede destine a produire des cellules et des tissus immunocompatibles par techniques de transfert nucleaire
EP1214443A1 (fr) * 1999-09-07 2002-06-19 Advanced Cell Technology, Inc. Restoration du telomere et extension de la duree de vie cellulaire dans les animaux clones a partir de cellules somatiques senescentes
WO2002102997A2 (fr) * 2000-11-30 2002-12-27 Stemron Inc. Cellules souches homozygotes isolees, cellules differenciees derivees de ces cellules souches, et materiaux et procedes permettant de les fabriquer et de les utiliser
WO2003040359A1 (fr) * 2001-11-06 2003-05-15 Shanghai Second Medical University Preparation d'un embryon somatique au moyen d'un ovocyte de lapin
JP2004500066A (ja) * 1999-12-20 2004-01-08 ユニバシティ　オブ　マサチューセッツ、ア　パブリック　インスチチユーション　オブ　ハイアー　エデュケイション　オブ　ザ　コモンウエルス　オブ　マサチューセッツ、アズ　リプリゼンテッド　バイ　イッツ　ア 異種間核移植により生成する胚あるいは幹様細胞
EP1621610A1 (fr) * 2003-04-08 2006-02-01 Southeast University Procede de production de cellules souches embryonnaires au moyen d'une technique de transfert de noyau de cellule somatique
EP2270504A2 (fr) 2000-01-11 2011-01-05 Geron Corporation Méthodes de culture et de différentiation de cellules souches humaines pluripotentes.
US7964401B2 (en) 2004-02-19 2011-06-21 Kyoto University Screening method for somatic cell nuclear reprogramming substance affecting ECAT2 and ECAT3
US8071369B2 (en) 2003-11-26 2011-12-06 Whitehead Institute For Biomedical Research Compositions for reprogramming somatic cells
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
EP2626416A3 (fr) * 2007-04-07 2013-12-18 The Whitehead Institute for Biomedical Research Reprogrammation de cellules somatiques
US9499797B2 (en) 2008-05-02 2016-11-22 Kyoto University Method of making induced pluripotent stem cells
US9497943B2 (en) 2008-06-13 2016-11-22 Whitehead Institute For Biomedical Research Nucleic acid constructs encoding reprogramming factors linked by self-cleaving peptides
US9580683B2 (en) 1999-06-30 2017-02-28 Advanced Cell Technology, Inc. ES cell cytoplasm or ooplasm transfer to rejuventate recipient cells
US9714433B2 (en) 2007-06-15 2017-07-25 Kyoto University Human pluripotent stem cells induced from undifferentiated stem cells derived from a human postnatal tissue

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002330465B2 (en) * 2001-09-21 2006-07-27 Kyoto University Screening method for a reprogramming agent, reprogramming agent screened by the method, method for using the reprogramming agent, method for differentiating an undifferentiated fusion cell, and production method of cells, tissues and organs.

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994024274A1 (fr) * 1993-04-21 1994-10-27 The University Of Edinburgh Isolation, selection et propagation de cellules souches d'animaux transgeniques
WO1995020042A1 (fr) * 1994-01-25 1995-07-27 Ppl Therapeutics (Scotland) Limited Isolement de cellules souche embryonnaires
WO1997007669A1 (fr) * 1995-08-31 1997-03-06 Roslin Institute (Edinburgh) Populations de cellules quiescentes pour transfert de noyau
WO1999019469A1 (fr) * 1997-10-09 1999-04-22 Biotransplant, Inc. Cellules souches porcines comprenant un marqueur sous un promoteur oct-4

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994024274A1 (fr) * 1993-04-21 1994-10-27 The University Of Edinburgh Isolation, selection et propagation de cellules souches d'animaux transgeniques
WO1995020042A1 (fr) * 1994-01-25 1995-07-27 Ppl Therapeutics (Scotland) Limited Isolement de cellules souche embryonnaires
WO1997007669A1 (fr) * 1995-08-31 1997-03-06 Roslin Institute (Edinburgh) Populations de cellules quiescentes pour transfert de noyau
WO1999019469A1 (fr) * 1997-10-09 1999-04-22 Biotransplant, Inc. Cellules souches porcines comprenant un marqueur sous un promoteur oct-4

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
J.A. THOMSON ET AL.: "EMBRYONIC STEN CELL LUNES DERIVED FROM HUMAN BLASTOCYSTS." SCIENCE, vol. 282, 6 November 1998 (1998-11-06), pages 1145-1147, XP002121340 LANCASTER, PA, US cited in the application *

Cited By (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9580683B2 (en) 1999-06-30 2017-02-28 Advanced Cell Technology, Inc. ES cell cytoplasm or ooplasm transfer to rejuventate recipient cells
EP1214403A1 (fr) * 1999-09-07 2002-06-19 Advanced Cell Technology, Inc. Procede destine a produire des cellules et des tissus immunocompatibles par techniques de transfert nucleaire
EP1214443A1 (fr) * 1999-09-07 2002-06-19 Advanced Cell Technology, Inc. Restoration du telomere et extension de la duree de vie cellulaire dans les animaux clones a partir de cellules somatiques senescentes
EP1214443A4 (fr) * 1999-09-07 2003-08-27 Advanced Cell Tech Inc Restoration du telomere et extension de la duree de vie cellulaire dans les animaux clones a partir de cellules somatiques senescentes
EP1214403A4 (fr) * 1999-09-07 2005-03-09 Advanced Cell Tech Inc Procede destine a produire des cellules et des tissus immunocompatibles par techniques de transfert nucleaire
JP2004500066A (ja) * 1999-12-20 2004-01-08 ユニバシティ　オブ　マサチューセッツ、ア　パブリック　インスチチユーション　オブ　ハイアー　エデュケイション　オブ　ザ　コモンウエルス　オブ　マサチューセッツ、アズ　リプリゼンテッド　バイ　イッツ　ア 異種間核移植により生成する胚あるいは幹様細胞
EP2270504A2 (fr) 2000-01-11 2011-01-05 Geron Corporation Méthodes de culture et de différentiation de cellules souches humaines pluripotentes.
WO2002102997A2 (fr) * 2000-11-30 2002-12-27 Stemron Inc. Cellules souches homozygotes isolees, cellules differenciees derivees de ces cellules souches, et materiaux et procedes permettant de les fabriquer et de les utiliser
WO2002102997A3 (fr) * 2000-11-30 2003-12-24 Stemron Inc Cellules souches homozygotes isolees, cellules differenciees derivees de ces cellules souches, et materiaux et procedes permettant de les fabriquer et de les utiliser
WO2003040359A1 (fr) * 2001-11-06 2003-05-15 Shanghai Second Medical University Preparation d'un embryon somatique au moyen d'un ovocyte de lapin
EP1621610A1 (fr) * 2003-04-08 2006-02-01 Southeast University Procede de production de cellules souches embryonnaires au moyen d'une technique de transfert de noyau de cellule somatique
EP1621610A4 (fr) * 2003-04-08 2007-02-14 Univ Southeast Procede de production de cellules souches embryonnaires au moyen d'une technique de transfert de noyau de cellule somatique
US8071369B2 (en) 2003-11-26 2011-12-06 Whitehead Institute For Biomedical Research Compositions for reprogramming somatic cells
US10017744B2 (en) 2003-11-26 2018-07-10 Whitehead Institute For Biomedical Research Methods for reprogramming somatic cells
US8927279B2 (en) 2003-11-26 2015-01-06 Whitehead Institute For Biomedical Research Methods for reprogramming somatic cells
US8932856B2 (en) 2003-11-26 2015-01-13 Whitehead Institute For Biomedical Research Methods for reprogramming somatic cells
US8940536B2 (en) 2003-11-26 2015-01-27 Whitehead Institute For Biomedical Research Methods for making somatic cells more susceptible to reprogramming
US8951797B2 (en) 2003-11-26 2015-02-10 Whitehead Institute For Biomedical Research Compositions for identifying reprogramming factors
US11987815B2 (en) 2003-11-26 2024-05-21 Whitehead Institute For Biomedical Research Methods for reprogramming somatic cells
US11655459B2 (en) 2003-11-26 2023-05-23 Whitehead Institute For Biomedical Research Methods for reprogramming somatic cells
US10457917B2 (en) 2003-11-26 2019-10-29 Whitehead Institute For Biomedical Research Methods for reprogramming somatic cells
US9670464B2 (en) 2003-11-26 2017-06-06 Whitehead Institute For Biomedical Research Methods for reprogramming somatic cells
US7964401B2 (en) 2004-02-19 2011-06-21 Kyoto University Screening method for somatic cell nuclear reprogramming substance affecting ECAT2 and ECAT3
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
EP2626416A3 (fr) * 2007-04-07 2013-12-18 The Whitehead Institute for Biomedical Research Reprogrammation de cellules somatiques
US9714414B2 (en) 2007-04-07 2017-07-25 Whitehead Institute For Biomedical Research Reprogramming of somatic cells
US10093904B2 (en) 2007-04-07 2018-10-09 Whitehead Institute For Biomedical Research Reprogramming of somatic cells
US9382515B2 (en) 2007-04-07 2016-07-05 Whitehead Institute For Biomedical Research Reprogramming of somatic cells
US9714433B2 (en) 2007-06-15 2017-07-25 Kyoto University Human pluripotent stem cells induced from undifferentiated stem cells derived from a human postnatal tissue
US9499797B2 (en) 2008-05-02 2016-11-22 Kyoto University Method of making induced pluripotent stem cells
US9497943B2 (en) 2008-06-13 2016-11-22 Whitehead Institute For Biomedical Research Nucleic acid constructs encoding reprogramming factors linked by self-cleaving peptides

Also Published As

Publication number Publication date
GB9809178D0 (en) 1998-07-01
WO1999055841A3 (fr) 2000-01-06
JP2002512787A (ja) 2002-05-08
EP1073719A2 (fr) 2001-02-07
MXPA00010473A (es) 2002-04-24
AU3621299A (en) 1999-11-16
CA2329975A1 (fr) 1999-11-04
IL139322A0 (en) 2001-11-25

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