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WO1999037779A1 - Conjugues fragments d'anticorps-polymeres et anticorps monoclonaux humanises anti-il-8, et leurs utilisations - Google Patents

Conjugues fragments d'anticorps-polymeres et anticorps monoclonaux humanises anti-il-8, et leurs utilisations Download PDF

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Publication number
WO1999037779A1
WO1999037779A1 PCT/US1999/001081 US9901081W WO9937779A1 WO 1999037779 A1 WO1999037779 A1 WO 1999037779A1 US 9901081 W US9901081 W US 9901081W WO 9937779 A1 WO9937779 A1 WO 9937779A1
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WIPO (PCT)
Prior art keywords
antibody fragment
conjugate
fab
peg
molecule
Prior art date
Application number
PCT/US1999/001081
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English (en)
Inventor
Vanessa Hsei
Iphigenia Koumenis
Steven J. Leong
Leonard G. Presta
Zahra Shahrokh
Gerardo A. Zapata
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Genentech, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Priority claimed from PCT/US1998/003337 external-priority patent/WO1998037200A2/fr
Priority claimed from US09/121,952 external-priority patent/US6458355B1/en
Application filed by Genentech, Inc. filed Critical Genentech, Inc.
Priority to AU25597/99A priority Critical patent/AU2559799A/en
Publication of WO1999037779A1 publication Critical patent/WO1999037779A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/244—Interleukins [IL]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
    • A61K47/59—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes
    • A61K47/60—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
    • A61K47/6835—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment the modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site
    • A61K47/6845—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment the modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site the antibody targeting a cytokine, e.g. growth factors, VEGF, TNF, a lymphokine or an interferon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
    • A61K47/6835—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment the modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site
    • A61K47/6883—Polymer-drug antibody conjugates, e.g. mitomycin-dextran-Ab; DNA-polylysine-antibody complex or conjugate used for therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/54—F(ab')2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Definitions

  • This application relates to the field of antibody fragments derivatized with polymers, and in particular to the use of such derivatization to increase the circulation half-lives of antibody fragment-polymer conjugates.
  • This application also relates to the field of inflammatory diseases and asthma, and in particular to anti-IL-8 antibody treatment of inflammatory diseases and asthmatic diseases.
  • This application further relates to humanized anti-interleukin-8 (IL-8) antibodies and to high affinity variants of such antibodies.
  • PEGylation Modification of proteins with polyethylene glycol
  • PEGylation has not been widely exploited for clinical applications.
  • antibody fragments PEGylation has not been shown to extend serum half-life to useful levels. Delgado et al., Br. J. Cancer.
  • Zapata et al., FASEB J., 9: A 1479 (1995) reported that low molecular weight (5 or 10 kD) PEG attached to a sulfhydryl group in the hinge region of a Fab' fragment reduced clearance compared to the parental Fab' molecule.
  • Interleukin-8 is neutrophil chemotactic peptide secreted by a variety of cells in response to inflammatory mediators (for a review see Hebert et al. Cancer Investigation 1 1(6):743 (1993)). IL-8 can play an important role in the pathogenesis of inflammatory disorders, such as adult respiratory distress syndrome (ARDS), septic shock, and multiple organ failure. Immune therapy for such inflammatory disorders can include treatment of an affected patient with anti-IL-8 antibodies.
  • Sticherling et al. J. Immunol. 143:1628 (1989) disclose the production and characterization of four monoclonal antibodies against IL-8.
  • WO 92/04372, published March 19, 1992 discloses polyclonal antibodies which react with the receptor-interacting site of IL-8 and peptide analogs of IL-8, along with the use of such antibodies to prevent an inflammatory response in patients.
  • St. John et al. (Chest 103:932 (1993)) review immune therapy for ARDS, septic shock, and multiple organ failure, including the potential therapeutic use of anti-IL-8 antibodies. Sekido et al.
  • WO 95/23865 International Application No. PCT/US95/02589 published September 8, 1995 demonstrates that anti-IL-8 monoclonal antibodies can be used therapeutically in the treatment of other inflammatory disorders, such as bacterial pneumonias and inflammatory bowel disease.
  • Anti-IL-8 antibodies are additionally useful as reagents for assaying IL-8.
  • Sticherling et al. disclose the use of anti-IL-8 monoclonal antibodies as reagents in immunohistochemical studies.
  • Ko et al. disclose the use of anti-IL-8 monoclonal antibodies as reagents in an enzyme-linked immunoabsorbent assay (ELISA) for IL-8.
  • ELISA enzyme-linked immunoabsorbent assay
  • One aspect of the invention is a conjugate consisting essentially of one or more antibody fragments covalently attached to one or more nonproteinaceous polymer molecules, wherein the apparent size of the conjugate is at least about 500 kD.
  • Another aspect of the invention is a conjugate formed by one or more antibody fragments covalently attached to one or more nonproteinaceous polymer molecules, wherein the apparent size of the conjugate is at least about 500 kD, and wherein the covalent structure of the conjugate is free of any matter other than the antibody fragment and nonproteinaceous polymer molecules.
  • Yet another aspect of the invention is a conjugate formed by the one or more antibody fragments covalently attached to one or more nonproteinaceous polymer molecules, wherein the covalent structure of the conjugate further incorporates one or more nonproteinaceous labels, wherein the covalent structure of the conjugate is free of any matter other than the antibody fragment, nonproteinaceous polymer and nonproteinaceous label molecules, and wherein the apparent size ofthe conjugate is at least about 500 kD.
  • An additional aspect of the invention is a method of treating an inflammatory disorder in a mammal comprising administering to the mammal an effective amount of a conjugate consisting essentially of one or more antibody fragments covalently attached to one or more nonproteinaceous polymer molecules, wherein at least one antibody fragment comprises an antigen binding site that binds to human interleukin-8 (IL-8), and wherein the apparent size ofthe conjugate is at least about 500 kD.
  • IL-8 human interleukin-8
  • a further aspect of the invention is a method of treating an asthmatic disorder in a mammal comprising administering to the mammal an effective amount of a conjugate consisting essentially of one or more antibody fragments covalently attached to one or more nonproteinaceous polymer molecules, wherein at least one antibody fragment comprises an antigen binding site that binds to human interleukin-8 (IL-8), and wherein the apparent size ofthe conjugate is at least about 500 kD.
  • IL-8 human interleukin-8
  • FIGURES Figure 1 is a graph depicting the blocking of IL-8 mediated elastase release from neutrophils by anti- IL-8 monoclonal antibody 5.12.14.
  • Figure 2 is a graph depicting the inhibition of I-IL-8 binding to neutrophils by unlabeled IL-8.
  • Figure 3 demonstrates that a isotype matched negative control Fab (denoted as "4D5 Fab") does not
  • Figure 4 is a graph depicting the inhibition of binding of I-IL-8 to human neutrophils by chimeric
  • FIG. 5 is a graph depicting the inhibition of binding of I-IL-8 to human neutrophils by chimeric 6G.4.25 Fab with an average IC 50 of 7.5 nM.
  • Figure 6 demonstrates the inhibition of human IL-8 mediated neutrophil chemotaxis by chimeric 6G4.2.5 Fab and chimeric 5.12.14 Fab.
  • Figure 7 demonstrates the relative abilities of chimeric 6G4.2.5 Fab and chimeric 5.12.14 Fab to inhibit rabbit IL-8 mediated neutrophil chemotaxis.
  • Figure 8 depicts the stimulation of elastase release from human neutrophils by various concentrations of human and rabbit IL-8.
  • the relative extent of elastase release was quantitated by measurement of absorbance at 405 nm.
  • the data represent mean + SEM of triplicate samples.
  • Figure 9 is a graph depicting the ability of chimeric 6G4.2.5 Fab and chimeric 5.12.14 Fab to inhibit elastase release from human neutrophils stimulated by human IL-8. The results were normalized to reflect the percentage of elastase release elicited by 100 nM IL-8 alone. The data represent the mean + SEM of three separate experiments performed on different days with different blood donors. IC 50 values were calculated by four parameter fit.
  • Figure 10 is a graph depicting the relative abilities of chimeric 6G4.2.5 Fab and chimeric 5.12.14 Fab to inhibit elastase release from human neutrophils stimulated by rabbit IL-8. The results were normalized to reflect the percentage of elastase release elicited by 100 nM IL-8 alone. The data represent the mean + SEM of three separate experiments performed on different days with different blood donors. IC 50 values were calculated by four parameter fit.
  • Figures 1 1A-1 1J are a set of graphs depicting the following parameters in a rabbit ulcerative colitis model: Figure 1 1A depicts myeloperoxidase levels in tissue; Figure 1 IB depicts IL-8 levels in tissue; Figure
  • FIG. 11C depicts colon weight; Figure 1 ID depicts gross inflammation; Figure HE depicts edema; Figure 1 1 F depicts extent of necrosis; Figure 1 IG depicts severity of necrosis; Figure 1 1H depicts neutrophil margination; Figure 1 11 depicts neutrophil infiltration; and Figure 1 1 J depicts mononuclear infiltration.
  • Figure 12 is a graph depicting the effect of anti-IL-8 monoclonal antibody treatment on the number of neutrophils in bronchoalveolar lavage (BAL) fluid in animals infected with Streptococcus pneumoniae. Escherichia coli. or Pseudomonas aeruginosa. Treatment with 6G4.2.5 significantly reduced the number of neutrophils present in the BAL fluid compared to animals treated with isotype control mouse IgG ( Figure 12).
  • Figure 13 depicts the DNA sequences (SEQ ID NOS: 1-6) of three primers designed for each of the light and heavy chains. Multiple primers were designed in order to increase the chances of primer hybridization and efficiency of first strand cDNA synthesis for cloning the variable light and heavy regions of monoclonal antibody 5.12.14.
  • Figure 14 depicts the DNA sequences (SEQ ID NOS: 7-10) of one forward primer and one reverse primer for the 5.12.14 light chain variable region amplification.
  • Figure 15 depicts the DNA sequences (SEQ ID NOS: 11-15) of one forward primer and one reverse primer for the 5.12.14 heavy chain variable region amplification.
  • Figure 16 depicts the DNA sequence (SEQ ID NO: 16) and the amino acid sequence (SEQ ID NO: 17) of the 5.12.14 light chain variable region and partial murine constant light region.
  • CDRs are indicated by either X-ray crystallography (underlined amino acids) or by Kabat sequence comparison (amino acids denoted with asterisk). Important restriction sites are indicated in italics.
  • the signal peptide of STII is amino acids -23 to -1.
  • the murine variable light region is amino acids 1 to 109.
  • the partial murine constant light region is amino acids 110 to 123 (in italics).
  • Figure 17 depicts the DNA sequence (SEQ ID NO: 18) and the amino acid sequence (SEQ ID NO: 19) of the 5.12.14 heavy chain variable region and partial murine constant heavy region.
  • CDRs are indicated by either X-ray crystallography (underlined amino acids) or by Kabat sequence comparison (amino acids denoted with asterisk). Important restriction sites are indicated in italics.
  • the signal peptide of STII is amino acids -23 to -1.
  • the murine variable heavy region is amino acids 1 to 120.
  • the partial murine constant heavy region is amino acids 121 to 130.
  • Figure 18 depicts the DNA sequences (SEQ ID NOS: 20-23) of amplification primers used to convert murine light and heavy chain constant region residues to their human equivalents.
  • Figure 19 depicts the DNA sequence (SEQ ID NO: 24) and the amino acid sequence (SEQ ID NO: 25) for the 5.12.14 light chain variable region and the human IgGl light chain constant region.
  • CDRs are indicated by either X-ray crystallography (underlined amino acids) or by Kabat sequence comparison (amino acids denoted with asterisk).
  • the human constant region is denoted in italics.
  • the signal peptide of STII is amino acids -23 to -1.
  • the murine variable light region is amino acids 1 to 109.
  • the human constant light region is amino acids 1 10 to 215.
  • Figures 20A-20B depict the DNA sequence (SEQ ID NO: 26) and the amino acid sequence (SEQ ID NO: 26) and the amino acid sequence (SEQ ID NO:
  • Figure 21 depicts the DNA sequences (SEQ ID NOS: 1-6) of three primers designed for each of the light and heavy chains. Multiple primers were designed in order to increase the chances of primer hybridization and efficiency of first strand cDNA synthesis for cloning the variable light and heavy regions of monoclonal antibody 6G4.2.5.
  • Figure 22 depicts the DNA sequences (SEQ ID NOS: 28-31) of one forward primer and one reverse primer for the 6G4.2.5 light chain variable region amplification.
  • Figure 23 depicts the DNA sequences (SEQ ID NOS: 32,33,1 1,15,14, and 13) of one forward primer and one reverse primer for the 6G4.2.5 heavy chain variable region amplification.
  • Figure 24 depicts the DNA sequence (SEQ ID NO: 34) and the amino acid sequence (SEQ ID NO: 35) ofthe 6G4.2.5 light chain variable region and partial murine constant light region.
  • CDRs are indicated by either X-ray crystallography (underlined amino acids) or by Kabat sequence comparison (amino acids denoted with asterisk). Useful cloning sites are in italics.
  • the signal peptide of STII is amino acids -23 to -1.
  • the murine variable light region is amino acids 1 to 1 14.
  • the partial murine constant light region is amino acids 115 to 131.
  • Figure 25 depicts the DNA sequence (SEQ ID NO: 36) and the amino acid sequence (SEQ ID NO:
  • CDRs are indicated by either X-ray crystallography (underlined amino acids) or by Kabat sequence comparison (amino acids denoted with asterisk). Useful cloning sites are in italics.
  • the signal peptide of STII is amino acids -23 to -1.
  • the murine variable heavy region is amino acids 1 to 122.
  • the partial murine constant heavy region is amino acids 123 to 135.
  • Figure 26 depicts the DNA sequences (SEQ ID NOS: 38-40) of primers to convert the murine light chain and heavy chain constant regions to their human equivalents.
  • Figures 27A-27B depict the DNA sequence (SEQ ID NO: 41) and the amino acid sequence (SEQ ID NO: 42) for the chimeric 6G4.2.5 light chain.
  • CDRs are indicated by either X-ray crystallography (underlined amino acids) or by Kabat sequence comparison (amino acids denoted with asterisk).
  • the human constant region is denoted in italics.
  • the signal peptide of STII is amino acids -23 to - 1.
  • the murine variable heavy region is amino acids 1 to 1 14.
  • the human constant heavy region is amino acids 115 to 220.
  • Figures 28A-28B depict the DNA sequence (SEQ ID NO: 43) and the amino acid sequence (SEQ ID NO: 44) for the chimeric 6G4.2.5 heavy chain. CDRs are indicated by either X-ray crystallography (underlined amino acids) or by Kabat sequence comparison (amino acids denoted with asterisk).
  • the human constant region is denoted in italics.
  • the signal peptide of STII is amino acids -23 to -1.
  • the murine variable heavy region is amino acids 1 to 122.
  • the human constant heavy region is amino acids 123 to 231.
  • Fig. 29 depicts an amino acid sequence alignment of murine 6G425 light chain variable domain (SEQ ID NO: 45), humanized 6G425 F(ab)-1 light chain variable domain (SEQ ID NO: 46), and human light chain ⁇ l consensus framework (SEQ ID NO: 47) amino acid sequences, and an amino acid sequence alignment of murine 6G425 heavy chain variable domain (SEQ ID NO: 48), humanized 6G425 F(ab)-1 heavy chain variable domain (SEQ ID NO: 49), and human IgGl subgroup III heavy chain variable domain (SEQ ID NO: 50) amino acid sequences, used in the humanization of 6G425.
  • Figs. 30A, 30B and 30C are graphs depicting the ability of F(ab)-9 (humanized 6G4V1 1 Fab) to inhibit human wild type IL-8, human monomeric IL-8, and rhesus IL-8 mediated neutrophil chemotaxis, respectively.
  • Fig. 30A presents inhibition data for F(ab)-9 samples at concentrations of 0.06 nM, 6.25 nM, 12.5 nM, 25 nM, 50 nM, and 100 nM, for an isotype control antibody (denoted "4D5") sample at a concentration of 100 nM, and for a no antibody control sample, in the presence of 2nM human wild type IL-8.
  • Fig. 30A presents inhibition data for F(ab)-9 samples at concentrations of 0.06 nM, 6.25 nM, 12.5 nM, 25 nM, 50 nM, and 100 nM, for an isotype control antibody (denoted "4D5") sample
  • 30B presents inhibition data for F(ab)-9 samples at concentrations of 6.25 nM, 12.5 nM, 25 nM, and 50 nM, for an isotype control antibody (denoted “4D5") sample at a concentration of 100 nM, and for a no antibody control sample, in the presence of 4 nM human monomeric IL-8 (denoted as “BD59” and as “monomeric IL-8").
  • 4D5 isotype control antibody
  • BD59 human monomeric IL-8
  • FIG. 30C presents inhibition data for F(ab)-9 samples at concentrations of 1 nM, 12.5 nM, 25 nM, and 50 nM, for an isotype control antibody (denoted “4D5") sample at a concentration of 100 nM, and for a no antibody control sample, in the presence of 2 nM rhesus IL-8.
  • Figs. 30A-30C each presents data for a no IL-8 buffer control sample (denoted as "Buffer”) in the respective inhibition assay.
  • Fig. 31A depicts the amino acid sequences of the humanized anti-IL-8 6G4.2.5V1 1 light chain in an N-terminal fusion with the STII leader peptide (SEQ ID NO: 51), the humanized anti-IL-8 6G4.2.5V11 heavy chain in an N-terminal fusion with the STII leader peptide (SEQ ID NO: 52), and a peptide linker in a C- terminal fusion with Ml 3 phage gene-III coat protein (SEQ ID NO: 53).
  • Fig. 31A depicts the amino acid sequences of the humanized anti-IL-8 6G4.2.5V1 1 light chain in an N-terminal fusion with the STII leader peptide (SEQ ID NO: 51), the humanized anti-IL-8 6G4.2.5V11 heavy chain in an N-terminal fusion with the STII leader peptide (SEQ ID NO: 52), and a peptide linker in a C- terminal fusion with Ml 3 phage gene-III coat protein
  • 31 B depicts the nucleic acid sequence (SEQ ID NO: 54) and the translated amino acid sequence (SEQ ID NO: 51) of the humanized anti-IL-8 6G4.2.5V1 1 light chain in an N-terminal fusion with the STII leader peptide.
  • Fig. 31C depicts the amino acid sequences of the humanized anti-IL-8 6G4.2.5V19 light chain in an N-terminal fusion with the STII leader peptide (SEQ ID NO: 51), and the humanized anti-IL-8 6G4.2.5V19 heavy chain in an N-terminal fusion with the STII leader peptide (SEQ ID NO: 55).
  • Fig. 32 is a three dimensional computer model of the humanized anti-IL-8 6G4.2.5V1 1 antibody. Heavy chain CDR loops and variable domain regions appear in purple, and CDR-H3 side chain residues appear in yellow. Heavy chain constant domain regions appear in red. Light chain CDR loops and variable domain regions appear in off-white, and the Asn residue at amino acid position 35 (N35) in CDR LI appears in green. Light chain constant domain regions appear in amber.
  • Fig. 33 is a Scatchard plot depicting the inhibition of I-IL-8 binding to human neutrophils exhibited by intact murine 6G4.2.5 antibody (denoted 6G4 murine mAb), 6G4.2.5 murine-human chimera Fab (denoted 6G4 chimera), humanized 6G4.2.5 Fab versions 1 and 1 1 (denoted VI and VI 1), and variant 6G4.2.5V11N35A Fab (denoted VI 1N35A).
  • Figs. 34A, 34B, 34C and 34D are graphs depicting the ability of 6G4.2.5V1 1N35A Fab to inhibit human wild type IL-8, human monomeric IL-8, rabbit IL-8, and rhesus IL-8 mediated neutrophil chemotaxis, respectively.
  • Fig. 34A presents inhibition data for 6G4.2.5V11N35A Fab samples at concentrations of 0.5, 1, 2, 4, 8, 16, and 33 nM, for an isotype control antibody (denoted "4D5") sample at a concentration of 33 nM, and for a no antibody control (denoted "HuIL-8") sample, in the presence of 2 nM human wild type IL-8.
  • Fig. 34A presents inhibition data for 6G4.2.5V11N35A Fab samples at concentrations of 0.5, 1, 2, 4, 8, 16, and 33 nM, for an isotype control antibody (denoted "4D5") sample at a concentration of 33 nM, and for a no antibody control (
  • 34B presents inhibition data for 6G4.2.5V11N35A Fab samples at concentrations of 0.5, 1, 2, 4, 8, 16, and 33 nM, for an intact 6G4.2.5 mAb sample at a concentration of 33 nM, for an isotype control antibody (denoted as "4D5") sample at a concentration of 33 nM, and for a no antibody control (denoted "BD59") sample, in the presence of 2 nM human monomeric IL-8.
  • Fig. 34C presents inhibition data for 6G4.2.5V11N35A Fab samples at concentrations of 0.5, 1, 2, 4, 8, 16, and 33 nM.
  • FIG. 34D presents inhibition data for 6G4.2.5V11N35A Fab samples at concentrations of 0.5, 1, 2, 4, 8, 16, and 33 nM, for an intact 6G4.2.5 mAb sample at a concentration of 33 nM, for an isotype control antibody (denoted as “4D5") sample at a concentration of 33 nM, and for a no antibody control (denoted "Rhe IL-8”) sample, in the presence of 2 nM rhesus IL-8.
  • Figs. 34B-34D each presents data for human wild type IL-8 control (denoted "HuIL-8") samples at a concentration of 2 nM in the respective assay
  • Figs. 34A-34D each presents data for a no IL-8 buffer control (denoted "Buffer”) sample in the respective assay.
  • Fig. 35 depicts the amino acid sequences of the humanized anti-IL-8 6G4.2.5V11N35A light chain in an N-terminal fusion with the STII leader peptide (SEQ ID NO: 56), the humanized anti-IL-8 6G4.2.5V11N35A heavy chain in an N-terminal fusion with the STII leader peptide (SEQ ID NO: 52), and the GCN4 leucine zipper peptide (SEQ ID NO: 57).
  • the Ala residue (substituted for the wild type Asn residue) at amino acid position 35 in the 6G4.2.5V1 1N35A light chain appears in bold case.
  • a putative pepsin cleavage site in the GCN4 leucine zipper sequence is underlined.
  • Fig. 36 depicts the DNA sequence (SEQ ID NO: 58) and the amino acid sequence (SEQ ID NO: 56) ofthe humanized anti-IL-8 6G4.2.5V11N35A light chain in an N-terminal fusion with the STII leader peptide.
  • Complementarity determining regions LI, L2, and L3 are underlined
  • Figs. 37A-37B depict the DNA sequence (SEQ ID NO: 59) and the amino acid sequence (SEQ ID NO:
  • Fig. 38 is a Scatchard plot depicting the inhibition of I-IL-8 binding to human neutrophils exhibited by 6G4.2.5V11N35A Fab (denoted Fab), 6G4.2.5V11N35A F(ab') 2 (denoted F(ab') 2 ), and human wild type IL-8 control (denoted IL-8).
  • Fig. 39 is a graph depicting a comparison of the wild type human IL-8 mediated neutrophil chemotaxis inhibition activities of the 6G4.2.5V1 1N35A F(ab') and 6G4.2.5V11N35A Fab.
  • Inhibition data are presented for 6G4.2.5V1 1N35A Fab samples (denoted "N35A Fab") and 6G4.2.5V11N35A F(ab') 2 samples (denoted N35A F(ab') 2 ) at concentrations of 0.3, 1, 3, 10, 30, and 100 nM, for an isotype control antibody (denoted as "4D5") sample at a concentration of 100 nM, and for a no antibody control sample, in the presence of 2 nM human wild type IL-8.
  • inhibition data are presented for no IL-8 buffer control samples (denoted "Buffer").
  • Fig. 40 is a graph depicting the ability of 6G4.2.5V11N35A F(ab') 2 to inhibit human monomeric IL- 8, rhesus IL-8, and rabbit IL-8 mediated neutrophil chemotaxis.
  • Human monomeric IL-8 mediated neutrophil chemotaxis data are presented for 6G4.2.5V1 1N35A F(ab') 2 samples at concentrations of 0.3, 1, 3, and 10 nM, for an isotype control antibody (denoted as "4D5") sample at a concentration of 100 nM, and for a no antibody control sample (denoted as "BD59”), in the presence of human monomeric IL-8 (denoted as "BD59”) at a concentration of 0.5 nM.
  • Rhesus IL-8 mediated neutrophil chemotaxis data are presented for 6G4.2.5V1 1N35A F(ab') 2 samples at concentrations of 0.3, 1, 3, and 10 nM, and for a no antibody control sample, in the presence of rhesus IL-8 at a concentration of 2 nM.
  • Rabbit IL-8 mediated neutrophil chemotaxis data are presented for 6G4.2.5V11N35A F(ab') 2 samples at concentrations of 0.3, 1, 3, and 10 nM, and for a no antibody control sample, in the presence of rabbit IL-8 at a concentration of 2 nM.
  • inhibition data are presented for a no IL-8 buffer control sample (denoted as "Buffer") and for a 2 nM human wild type IL-8 (denoted as "HuIL-8").
  • Figs. 41A-41V depict the nucleic acid sequence (SEQ ID NO: 61) of the p6G4VHN35A.F(ab') 2 vector.
  • Fig. 42 depicts the nucleic acid sequences ofthe stop template primer (SEQ ID NO: 63) and the NNS randomization primer (SEQ ID NO: 64) used for random mutagenesis of amino acid position 35 in variable light chain CDR-L 1 of humanized antibody 6G4V 11.
  • Fig. 43 A is a table of data describing the frequencies of different phage display clones obtained from the randomization of amino acid position 35 in variable light chain CDR-L1 of humanized antibody 6G4V11.
  • Figs. 43B, 43C, 43D and 43E are graphs of displacement curves depicting the inhibition of I-IL-8 binding to neutrophils exhibited by the 6G4V1 1N35A, 6G4V1 1N35D, 6G4V11N35E and 6G4V11N35G Fab's.
  • Fig. 44 contains a graph depicting the typical kinetics of an anti-IL-8 antibody fragment
  • Fig. 44 also contains a table of data providing the equilibrium constant for 6G4V1 IN35A Fab binding to IL-8 (rate constants were not determined "ND"), and the equilibrium and rate constants for 6G4V1 1N35A F(ab') 2 and 6G4V11N35E Fab binding to IL-8.
  • Fig. 45 depicts the DNA sequence (SEQ ID NO: 65) and amino acid sequence (SEQ ID NO: 62) of the 6G4V1 1N35E light chain in an N-terminal fusion with the STII leader peptide. Complementarity determining regions LI, L2 and L3 are underlined.
  • Fig. 46 is a graph depicting the ability of 6G4V1 1N35E Fab to inhibit human IL-8 (dark columns) and rabbit IL-8 (light columns) mediated neutrophil chemotaxis. Data are presented for 6G4V1 1N35E Fab samples at concentrations of 0.4, 1.2, 3.7, 11 and 33 nM, and for an isotype control antibody (4D5) sample at a concentration of 100 nM, in the presence of 2 nM human IL-8 or 2 nM rabbit IL-8. In addition, inhibition data are presented for a no IL-8 buffer control sample (denoted "Buffer”) and for human and rabbit IL-8 control samples (denoted "IL-8").
  • Fig. 47 depicts the DNA sequence of the sense (SEQ ID NO: 66) and anti-sense (SEQ ID NO: 67) strands of a PvuII-XhoI synthetic nucleotide encoding amino acids Leu4 to Phe29 ofthe 6G4V11N35A heavy chain.
  • Figs. 48A-48T depict the DNA sequence (SEQ ID NO: 68) of plasmid p6G4Vl lN35A.choSD9.
  • Figs. 49A, 49B, 49C and 49D are graphs of displacement curves depicting the inhibition of I-IL-8 binding to neutrophils exhibited by IL-8 control, intact murine 6G4.2.5 antibody, the full length IgG 1 form of variant 6G4V11N35A, and the full length IgG 1 form of variant 6G4V11N35E, respectively.
  • Figs. 50A-50B are graphs depicting the ability of full length 6G4V1 1N35A IgGl and 6G4V11N35E IgGl to inhibit human IL-8 (Fig. 50A) and rabbit IL-8 (Fig. 50B) mediated neutrophil chemotaxis.
  • Fig. 51 contains a graph depicting the typical kinetics of a full length anti-IL8 antibody (6G4V11N35A IgGl) binding to IL-8.
  • Fig. 51 also contains a table of data providing the equilibrium and rate constants for full length murine 6G4.2.5 IgG2a, 6G4V11N35A IgGl and 6G4V1 1N35E IgGl binding to IL-8.
  • Figs. 52A and 52B are graphs of displacement curves depicting the results of an unlabeled IL-8/ I-
  • Fig. 53 depicts the DNA sequence (SEQ ID NO: 69) and amino acid sequence (SEQ ID NO: 70) of the 6G4V11N35A Fab' heavy chain (6G4V11N35A Fab heavy chain modified to contain a cysteine residue in the hinge region).
  • Figs. 54A-54C contain graphs of displacement curves depicting the IL-8 binding and IC 50 's for PEG- maleimide modified 6G4V1 1N35A Fab' molecules.
  • Figs. 55A-55C are graphs depicting the ability of PEG-maleimide modified 6G4V11N35A Fab' molecules to inhibit human IL-8 and rabbit IL-8 mediated neutrophil chemotaxis.
  • Figs. 56A-56C are graphs depicting the ability of PEG-maleimide modified 6G4V1 1N35A Fab' molecules to inhibit IL-8 mediated release of ⁇ -glucuronidase from neutrophils.
  • Figs. 57A-57B contain graphs of displacement curves depicting the inhibition of I-IL-8 binding to neutrophils exhibited by PEG-succinimide modified 6G4V11N35A Fab' 2 molecules.
  • Figs. 58A-58B are graphs depicting the ability of PEG-succinimide modified 6G4V1 1N35A F(ab') 2 molecules to inhibit human IL-8 mediated neutrophil chemotaxis.
  • Figs. 59A-59B are graphs depicting the ability of PEG-succinimide modified 6G4V1 1N35A F(ab') 2 molecules to inhibit human IL-8 mediated release of ⁇ -glucuronidase from neutrophils.
  • Fig. 60 is a graph depicting the theoretical molecular weight (dotted bars) and effective size (solid bars) of PEG-maleimide modified 6G4V1 1N35A Fab' molecules as determined by SEC-HPLC.
  • Figs. 61A and 61B are SDS-PAGE gels depicting the electrophoretic mobility of various PEG- maleimide modified 6G4V1 1N35A Fab' molecules under reducing and non-reducing conditions, respectively.
  • Fig. 62 contains size exclusion chromatograms (SEC-HPLC) depicting the retention times and effective (hydrodynamic) sizes of various PEG-succinimide modified 6G4V1 1N35A F(ab') 2 molecules.
  • Fig. 63 is a graph depicting the theoretical molecular weight (open columns), effective size determined by SEC-HPLC (solid columns), and the actual molecular weight determined by SEC-light scattering (shaded columns) for various PEG-succinimide modified 6G4V1 1N35A F(ab') 2 molecules.
  • Fig. 64 is an SDS-PAGE gel depicting the electrophoretic mobility of various PEG-succinimide modified 6G4V1 1N35A F(ab') 2 molecules. From left to right, lane 1 contains unmodified F(ab') 2 , lane 2 contains F(ab') 2 coupled to two 40 kD branched PEG-succinimide molecules (denoted "Br(2)-40kD(N)-
  • lane 3 contains F(ab') 2 coupled to one 40 kD branched PEG-succinimide molecule (denoted “Br(l)- 40kD-(N)-Fab'2")
  • lane 4 contains a mixture of F(ab') 2 coupled to four 20 kD linear PEG-succinimide molecules and F(ab') 2 coupled to five 20 kD linear PEG-succinimide molecules (denoted "L(4+5)-20kD-(N)-
  • lane 5 contains F(ab') 2 coupled to one 20 kD linear PEG-succinimide molecule (denoted "L(l)-20kD-
  • Figs. 65A and 65B are graphs comparing the serum concentration vs. time profiles of various PEG- maleimide modified 6G4V1 1N35A Fab' molecules (Fig. 65A) and various PEG-succinimide modified
  • Fig. 66 contains graphs comparing the serum concentration vs. time profiles of 6G4V1 1N35A Fab' coupled to one 40 kD branched PEG-maleimide molecule (denoted as "bran.(l)40K(s)Fab"'), 6G4V1 1N35A
  • Fig. 67 is a graph depicting the effect of 6G4V1 1N35A Fab' coupled to one 40 kD branched PEG- maleimide molecule (denoted as "PEG 40 Kd”) and murine anti-rabbit IL-8 monoclonal antibody 6G4.2.5 (full length IgG2a) (denoted as "6G4.2.5") on gross weight of entire lung in an ARDS rabbit model.
  • Fig. 68 is a graph depicting the effect of 6G4V1 1N35A Fab' coupled to one branched 40 kD PEG- maleimide molecule (denoted as "PEG 40 Kd” ⁇ and murine anti-rabbit IL-8 monoclonal antibody 6G4.2.5 (full length IgG2a) (denoted as "6G4.2.5") on BAL total leukocyte (light columns) and polymorphonuclear cell (dark columns) counts in an ARDS rabbit model. Untreated (no therapeutics) control animal data is denoted as "Control".
  • Fig. 69 is a graph depicting the effect of 6G4V1 1N35A Fab' coupled to one branched 40 kD PEG- maleimide molecule (denoted as "PEG 40 Kd”) ana murine anti-rabbit IL-8 monoclonal antibody 6G4.2.5 (full length IgG2a) (denoted as "6G4.2.5") on Pa02/Fi02 ratio at 24 hours-post treatment (light columns) and 48 hours post-treatment (dark columns) in an ARDS rabbit model. Untreated (no therapeutics) control animal data is denoted as "Control”.
  • Fig. 71 is a graph depicting the effect of pegylated anti-IL-8 Fab' (as measured by percent change in ear volume at 1, 2 and 3 days post reperfusion) in a rabbit ear model of ischemia reperfusion injury.
  • PCR Polymerase chain reaction
  • sequence information from the ends of the region of interest or beyond needs to be available, such that oligonucleotide primers can be designed; these primers will be identical or similar in sequence to opposite strands of the template to be amplified.
  • the 5' terminal nucleotides of the two primers can coincide with the ends of the amplified material.
  • PCR can be used to amplify specific RNA sequences, specific DNA sequences from total genomic DNA, and cDNA transcribed from total cellular RNA, bacteriophage or plasmid sequences, etc. See generally Mullis et al., Cold Spring Harbor Symp. Quant. Biol. 51:263 (1987); Erlich, ed., PCR Technology (Stockton Press, NY, 1989).
  • PCR is considered to be one, but not the only, example of a nucleic acid polymerase reaction method for amplifying a nucleic acid test sample comprising the use of a known nucleic acid as a primer and a nucleic acid polymerase to amplify or generate a specific piece of nucleic acid.
  • Antibodies are glycoproteins having the same structural characteristics. While antibodies exhibit binding specificity to a specific antigen, immunoglobulins include both antibodies and other antibody-like molecules which lack antigen specificity. Polypeptides of the latter kind are, for example, produced at low levels by the lymph system and at increased levels by myelomas.
  • “Native antibodies and immunoglobulins” are usually heterotetrameric glycoproteins of about 150,000 daltons, composed of two identical light (L) chains and two identical heavy (H) chains. Each light chain is linked to a heavy chain by one covalent disulfide bond, while the number of disulfide linkages varies between the heavy chains of different immunoglobulin isotypes. Each heavy and light chain also has regularly spaced intrachain disulfide bridges. Each heavy chain has at one end a variable domain (V H ) followed by a number of constant domains.
  • V H variable domain
  • Each light chain has a variable domain at one end (V L ) and a constant domain at its other end; the constant domain ofthe light chain is aligned with the first constant domain of the heavy chain, and the light chain variable domain is aligned with the variable domain of the heavy chain.
  • Particular amino acid residues are believed to form an interface between the light- and heavy-chain variable domains (Clothia et al., J. Mol. Biol. 186:651 (1985); Novot ⁇ y and Haber, Proc. Natl. Acad. Sci. U.S.A. 82:4592 (1985)).
  • variable refers to the fact that certain portions ofthe variable domains differ extensively in sequence among antibodies and are used in the binding and specificity of each particular antibody for its particular antigen. However, the variability is not evenly distributed throughout the variable domains of antibodies. It is concentrated in three segments called complementarity-determining regions (CDRs) or hypervariable regions both in the light-chain and the heavy-chain variable domains. The more highly conserved portions of variable domains are called the framework (FR).
  • CDRs complementarity-determining regions
  • FR framework
  • the variable domains of native heavy and light chains each comprise four FR regions, largely adopting a ⁇ -sheet configuration, connected by three CDRs, which form loops connecting, and in some cases forming part of, the ⁇ -sheet structure.
  • the CDRs in each chain are held together in close proximity by the FR regions and, with the CDRs from the other chain, contribute to the formation ofthe antigen-binding site of antibodies (see Kabat et al, Sequences of Proteins of Immunological Interest, Fifth Edition, National Institute of Health, Bethesda, MD (1991)).
  • the constant domains are not involved directly in binding an antibody to an antigen, but exhibit various effector functions, such as participation ofthe antibody in antibody-dependent cellular toxicity.
  • Papain digestion of antibodies produces two identical antigen-binding fragments, called “Fab” fragments, each with a single antigen-binding site, and a residual "Fc” fragment, whose name reflects its ability to crystallize readily. Pepsin treatment yields an F(ab') 2 fragment that has two antigen-combining sites and is still capable of cross-linking antigen.
  • Fv is the minimum antibody fragment which contains a complete antigen-recognition and -binding site.
  • this region consists of a dimer of one heavy- and one light-chain variable domain in tight, non-covalent association.
  • scFv single-chain Fv species
  • one heavy- and one light-chain variable domain can be covalently linked by a flexible peptide linker such that the light and heavy chains can associate in a "dimeric" structure analogous to that in a two-chain Fv species. It is in this configuration that the three CDRs of each variable domain interact to define an antigen-binding site on the surface of the VH-VL dimer.
  • the Fab fragment also contains the constant domain of the light chain and the first constant domain (CHI) of the heavy chain.
  • Fab' fragments differ from Fab fragments by the addition of a few residues at the carboxy terminus of the heavy chain CHI domain including one or more cysteines from the antibody hinge region.
  • Fab'-SH is the designation herein for Fab' in which the cysteine residue(s) ofthe constant domains bear a free thiol group.
  • F(ab ) 2 antibody fragments originally were produced as pairs of Fab' fragments which have hinge cysteines between them. Other chemical couplings of antibody fragments are also known.
  • the "light chains" of antibodies (immunoglobulins) from any vertebrate species can be assigned to one of two clearly distinct types, called kappa (k) and lambda (1), based on the amino acid sequences of their constant domains.
  • immunoglobulins can be assigned to different classes. There are five major classes of immunoglobulins: IgA, IgD, IgE, IgG, and IgM, and several of these can be further divided into subclasses (isotypes), e.g., IgG
  • the heavy-chain constant domains that correspond to the different classes of immunoglobulins are called a, ⁇ , e, ⁇ , and ⁇ , respectively.
  • the subunit structures and three-dimensional configurations of different classes of immunoglobulins are well known.
  • antibody is used in the broadest sense and specifically covers single monoclonal antibodies (including agonist and antagonist antibodies) and antibody compositions with polyepitopic specificity.
  • Antibody fragment and all grammatical variants thereof, as used herein are defined as a portion of an intact antibody comprising the antigen binding site or variable region of the intact antibody, wherein the portion is free of the constant heavy chain domains (i.e. CH2, CH3, and CH4, depending on antibody isotype) of the Fc region of the intact antibody.
  • constant heavy chain domains i.e. CH2, CH3, and CH4, depending on antibody isotype
  • antibody fragments include Fab, Fab', Fab'-SH, F(ab') 2 , and Fv fragments; diabodies; any antibody fragment that is a polypeptide having a primary structure consisting of one uninterrupted sequence of contiguous amino acid residues (referred to herein as a "single-chain antibody fragment” or “single chain polypeptide"), including without limitation ( 1 )single-chain Fv (scFv) molecules (2)single chain polypeptides containing only one light chain variable domain, or a fragment thereof that contains the three CDRs of the light chain variable domain, without an associated heavy chain moiety and (3)single chain polypeptides containing only one heavy chain variable region, or a fragment thereof containing the three CDRs of the heavy chain variable region, without an associated light chain moiety; and multispecific or multivalent structures formed from antibody fragments.
  • the heavy chain(s) can contain any constant domain sequence (e.g. CHI in the IgG isotype) found in a non-Fc region of an intact antibody, and/or can contain any hinge region sequence found in an intact antibody, and/or can contain a leucine zipper sequence fused to or situated in the hinge region sequence or the constant domain sequence of the heavy chain(s).
  • Suitable leucine zipper sequences include the jun and fos leucine zippers taught by Kostelney et al., J. Immunol., 148: 1547-1553 (1992) and the GCN4 leucine zipper described in the Examples below.
  • conjugate as described and claimed herein is defined as a heterogeneous molecule formed by the covalent attachment of one or more antibody fragment(s) to one or more polymer molecule(s), wherein the heterogeneous molecule is water soluble, i.e. soluble in physiological fluids such as blood, and wherein the heterogeneous molecule is free of any structured aggregate.
  • structured aggregate refers to (1) any aggregate of molecules in aqueous solution having a spheroid or spheroid shell structure, such that the heterogeneous molecule is not in a micelle or other emulsion structure, and is not anchored to a lipid bilayer, vesicle or liposome; and (2) any aggregate of molecules in solid or insolubilized form, such as a chromatography bead matrix, that does not release the heterogeneous molecule into solution upon contact with an aqueous phase.
  • conjugate encompasses the aforementioned heterogeneous molecule in a precipitate, sediment, bioerodible matrix or other solid capable of releasing the heterogeneous molecule into aqueous solution upon hydration ofthe solid.
  • polymer polymer molecule
  • nonproteinaceous polymer and “nonproteinaceous polymer molecule” are used interchangeably and are defined as a molecule formed by covalent linkage of two or more monomers, wherein none ofthe monomers is contained in the group consisting of alanine (Ala), cysteine (Cys), aspartic acid (Asp), glutamic acid (Glu), phenylalanine (Phe), glycine (Gly), histidine (His), isoleucine (He), lysine (Lys), leucine (Leu), methionine (Met), asparagine (Asn), proline (Pro), glutamine (Gin), arginine (Arg), serine (Ser), threonine (Thr), valine (Val), tryptophan (Trp), and tyrosine (Tyr) residues.
  • Al alanine
  • cysteine cysteine
  • Asp aspartic acid
  • Glu glutamic acid
  • Phe phenyla
  • mAb monoclonal antibody
  • monoclonal antibody refers to an antibody obtained from a population of substantially homogeneous antibodies, i.e., the individual antibodies comprising the population are identical except for possible naturally occurring mutations that may be present in minor amounts.
  • Monoclonal antibodies are highly specific, being directed against a single antigenic site.
  • polyclonal antibody preparations which typically include different antibodies directed against different determinants (epitopes)
  • each mAb is directed against a single determinant on the antigen.
  • the monoclonal antibodies are advantageous in that they can be synthesized by hybridoma culture, uncontaminated by other immunoglobulins.
  • the modifier "monoclonal” indicates the character of the antibody as being obtained from a substantially homogeneous population of antibodies, and is not to be construed as requiring production of the antibody by any particular method
  • the monoclonal antibodies to be used in accordance with the present invention may be made by the hybridoma method first described by Kohler et al , Nature, 256 495 (1975), or may be made by recombinant DNA methods (see, e g , U S Patent No 4,816,567 to Cabilly et al )
  • the “monoclonal antibodies” also include clones of antigen-recognition and binding-site containing antibody fragments (Fv clones) isolated from phage antibody libraries using the techniques described in Clackson et al Nature 352 624-628 (1991) and Marks et al . J Mol Biol , 222:581-597 (1991), for example
  • the monoclonal antibodies herein include hybrid and recombinant antibodies produced by splicing a variable (including hyperva ⁇ able) domain of an anti-IL-8 antibody with a constant domain (e g "humanized” antibodies), or a light chain with a heavy chain, or a chain from one species with a chain from another species, or fusions with heterologous proteins, regardless of species of origin or immunoglobulin class or subclass designation, as well as antibody fragments (e g , Fab, F(ab') 2 , and Fv), so long as they exhibit the desired biological activity (See, e g , U S Pat No 4,816,567 to Cabilly et al , Mage and Lamoyi, in Monoclonal Antibody Production Techniques and Applications, pp 79-97 (Marcel Dekker, Inc , New York, 1987) )
  • the monoclonal antibodies herein specifically include "chimeric" antibodies (immunoglobulins) in which a portion of the heavy and/or light chain is identical with or homologous to corresponding sequences in ant'bodies derived from a particular species or belonging to a particular antibody class or -ubclass, while the remainder of the cha ⁇ n(s) is identical with or homologous to corresponding sequences in antibodies denved from another species or belonging to another antibody class or subclass, as well as fragments of such antibodies, so long as they exhibit the desired biological activity (Cabilly et al , supra, Morrison et al , Proc Natl Acad Sci U S A 81 6851 (1984))
  • Humanized forms of non-human (e g , murine) antibodies are specific chimeric immunoglobulins, immunoglobulin chains or fragments thereof (such as Fv, Fab, Fab', F(ab') 2 , or other antigen-binding subsequences of antibodies) which contain minimal sequence derived from non-human immunoglobulin
  • humanized antibodies are human immunoglobulins (recipient antibody) in v.hich residues from a complementary-determining region (CDR) of the recipient are replaced by residues from a CDR of a non- human species (donor antibody) such as mouse, rat, or rabbit having the desired specificity, affinity, and capacity
  • donor antibody such as mouse, rat, or rabbit having the desired specificity, affinity, and capacity
  • Fv framework residues of the human immunoglobulin are replaced by corresponding non-human residues
  • humanized antibodies can comprise residues which are found neither in the recipient antibody nor in the imported CDR or framework sequences
  • Treatment refers to both therapeutic treatment and prophylactic or preventative measures. Those in need of treatment include those already with the disorder as well as those prone to have the disorder or those in which the disorder is to be prevented.
  • mammal for purposes of treatment refers to any animal classified as a mammal, including humans, domestic and farm animals, and zoo, sports, or pet animals, such as dogs, horses, cats, cows, etc.
  • the mammal herein is human.
  • protein, peptide and polypeptide are used interchangeably to denote an amino acid polymer or a set of two or more interacting or bound amino acid polymers.
  • inflammatory disorders refers to pathological states resulting in inflammation, typically caused by neutrophil chemotaxis.
  • disorders include inflammatory skin diseases including psoriasis and atopic dermatitis; systemic scleroderma and sclerosis; responses associated with inflammatory bowel disease (such as Crohn's disease and ulcerative colitis); ischemic reperfusion disorders including surgical tissue reperfusion injury, myocardial ischemic conditions such as myocardial infarction, cardiac arrest, reperfusion after cardiac surgery and constriction after percutaneous transluminal coronary angioplasty, stroke, and abdominal aortic aneurysms; cerebral edema secondary to stroke; cranial trauma; hypovolemic shock; asphyxia; adult respiratory distress syndrome; acute lung injury; Behcet's Disease; dermatomyositis; polymyositis; multiple sclerosis; dermatitis; meningitis; encephalitis; uveitis; osteoarthritis;
  • the preferre ⁇ indications include acute lung injury, adult respiratory distress syndrome, ischemic reperfusion (including surgical tissue reperfusion injury, myocardial ischemia, and acute myocardial infarction), hypovolemic shock, asthma, bacterial pneumonia and inflammatory bowel disease such as ulcerative colitis.
  • ischemic reperfusion including surgical tissue reperfusion injury, myocardial ischemia, and acute myocardial infarction
  • hypovolemic shock including surgical tissue reperfusion injury, myocardial ischemia, and acute myocardial infarction
  • asthma bacterial pneumonia
  • inflammatory bowel disease such as ulcerative colitis.
  • asthma refers to a condition of the lungs in which there is widespread narrowing of lower airways.
  • Atopic asthma and “allergic asthma” refer to asthma that is a manifestation of an IgE-mediated hypersensitivity reaction in the lower airways, including, e.g., moderate or severe chronic asthma, such as conditions requiring the frequent or constant use of inhaled or systemic steroids to control the asthma symptoms.
  • a preferred indication is allergic asthma.
  • hydrodynamic size refers to the size of a molecule as determined by comparison to a standard curve produced with globular protein molecular weight standards in a size exclusion chromatography system, wherein the standard curve is created by mapping the actual molecular weight of each standard against its elution time observed in the size exclusion chromatography system.
  • the apparent size of a test molecule is derived by using the molecule's elution time to extrapolate a putative molecular weight from the standard curve.
  • the molecular weight standards used to create the standard curve are selected such that the apparent size of the test molecule falls within the linear portion ofthe standard curve.
  • the invention arises from the surprising and unexpected discovery that antibody fragment- polymer conjugates having an effective or apparent size significantly greater than the antibody fragment- polymer conjugates described in the art confers an increase in serum half-life, an increase in mean residence time in circulation (MRT), and/or a decrease in serum clearance rate over underivatized antibody fragment which far exceed the modest changes in such biological property or properties obtained with the art-known antibody fragment-polymer conjugates.
  • MRT mean residence time in circulation
  • the present inventors have determined for the first time that increasing the effective size of an antibody fragment to at least about 500,000 D, or increasing the effective size of an antibody fragment by at least about 8 fold over the effective size of the parental antibody fragment, or derivatizing an antibody fragment with a polymer of at least about 20,000 D in molecular weight, yields a molecule with a commercially useful pharmacokinetic profile.
  • the greatly extended serum half-life, extended MRT, and/or reduced serum clearance rate of the conjugates of the invention makes such conjugates viable alternatives to intact antibodies used for therapeutic treatment of many disease indications.
  • Antibody fragments provide significant advantages over intact antibodies, notably the fact that recombinant antibody fragments can be made in bacterial cell expression systems. Bacterial cell expression systems provide several advantages over mammalian cell expression systems, including reduced time am cost at both the research and development and manufacturing stages of a product.
  • the present invention also arises from the humanization of the 6G4.2.5 murine anti- rabbit IL-8 monoclonal antibody ("6G4.2.5") described in WO 95/23865 (PCT/US95/02589 published September 8, 1995), the entire disclosure of which is specifically inco ⁇ orated herein by reference.
  • the hybridoma producing antibody 6G4.2.5 was deposited on September 28, 1994 with the American Type Culture Collection and assigned ATCC Accession No. HB 1 1722 as described in the Examples below.
  • the invention provides a humanized derivative of the 6G4.2.5 antibody, ' ariant 1 1 (referred to herein as "6G4.2.5vl l "), in which the murine CDRs of 6G4.2.5 are grafted onto a consensus framework for human light chain ⁇ l and human IgG 1 heavy chain subgroup III, followed by importing three framework residues from the murine 6G4.2.5 parent heavy chain variable domain sequence into analogous sites in the heavy chain variable domain of the human template sequence, as described in the Examples below.
  • the invention provides variants ofthe 6G4.2.5vl 1 antibody with certain amino acid substitution(s) yielding increased affinity for human IL-8 and/or promoting greater efficiency in recombinant manufacturing processes.
  • the invention provides an antibody fragment covalently attached to a polymer to form a conjugate having an effective or apparent size of at least about 500,000 Daltons (D). In another aspect, the invention provides an antibody fragment covalently attached to a polymer to form a conjugate having an apparent size that is at least about 8 fold greater than the apparent size of the parental antibody fragment. In yet another aspect, the invention provides an antibody fragment covalently attached to a polymer of at least about 20,000 D in molecular weight (MW).
  • MW molecular weight
  • the unexpectedly and surprisingly large increase in antibody fragment serum half-life, increase in MRT, and/or decrease in serum clearance rate can be achieved by using any type of polymer or number of polymer molecules which will provide the conjugate with an effective size of at least about 500,000 D, or by using any type of polymer or number of polymer molecules which will provide the conjugate with an effective size that is at least about 8 fold greater than the effective size of the parental antibody fragment, or by using any type or number of polymers wherein each polymer molecule is at least about 20,000 D in MW.
  • the invention is not dependent on the use of any particular polymer or molar ratio of polymer to antibody fragment in the conjugate.
  • the beneficial aspects of the invention extend to antibody fragments without regard to antigen specificity.
  • the antigen specificity of a given antibody will not substantially impair the extraordinary improvement in serum half-life, MRT, and/or serum clearance rate for antibody fragments thereof that can be obtained by derivatizing the antibody fragments as taught herein.
  • the conjugate has an effective size of at least about 500,000 D, or at least about 800,000 D, or at least about 900,000 D, or at least about 1,000,000 D, or at least about 1,200,000 D, or at least about 1 ,400,000 D, or at least about 1,500,000 D, or at least about 1,800,000 D, or at least about 2,000,000 D, or at least about 2,500,000 D.
  • the conjugate has an effective size of at or about 500,000 D to at or about 10,000,000 D, or an effective size of at or about 500,000 D to at or about 8,000,000 D, or an effective size of at or about 500,000 D to at or about 5,000,000 D, or an effective size of at or about 500,000 D to at or about 4,000,000 D, or an effective size of at or about 500,000 D to at or about 3,000,000 D, or an effective size of at or about 500,000 D to at or about 2,500,000 D, or an effective size of at or about 500,000 D to at or about 2,000,000 D, or an effective size of at or about 500,000 D to at or about 1,800,000 D, or an effective size of at or about 500,000 D to at or about 1,600,000 D, or an effective size of at or about 500,000 D to at or about 1,500,000 D, or an effective size of at or about 500,000 D to at or about 1,000,000 D.
  • the conjugate has an effective size of at or about 800,000 D to at or about 10,000,000 D, or an effective size of at or about 800,000 D to at or about 8,000,000 D, or an effective size of at or about 800,000 D to at or about 5,000,000 D, or an effective size of at or about 800,000 D to at or about 4,000,000 D, or an effective size of at or about 800,000 D to at or about 3,000,000 D, or an effective size of at or about 800,000 D to at or about 2,500,000 D, or an effective size of at or about 800,000 D to at or about 2,000,000 D, or an effective size of at or about 800,000 D to at or about 1,800,000 D, or an effective size of at or about 800,000 D to at or about 1,600,000 D, or an effective size of at or about 800,000 D to at or about 1,500,000 D, or an effective size of at or about 800,000 D to at or about 1,000,000 D.
  • the conjugate has an effective size of at or about 900,000 D to at or about 10,000,000 D, or an effective size of at or about 900,000 D to at or about 8,000,000 D, or an effective size of at or about 900,000 D to at or about 5,000,000 D, or an effective size of at or about 900,000 D to at or about 4,000,000 D, or an effective size of at or about 900,000 D to at or about 3,000,000 D, or an effective size of at or about 900,000 D to at or about 2,500,000 D, or an effective size of at or about 900,000 D to at or about 2,000,000 D, or an effective size of at or about 900,000 D to at or about 1,800,000 D, or an effective size of at or about 900,000 D to at or about 1,600,000 D, or an effective size of at or about 900,000 D to at or about 1,500,000 D.
  • the conjugate has an effective size of at or about 1 ,000,000 D to at or about 10,000,000 D, or an effective size of at or about 1,000,000 D to at or about 8,000,000 D, or an effective size of at or about 1,000,000 D to at or about 5,000,000 D, or an effective size of at or about 1,000,000 D to at or about 4,000,000 D, or an effective size of at or about 1,000,000 D to at or about 3,000,000 D, or an effective size of at or about 1,000,000 D to at or about 2,500,000 D, or an effective size of at or about 1,000,000 D to at or about 2,000,000 D, or an effective size of at or about 1,000,000 D to at or about 1,800,000 D, or an effective size of at or about 1,000,000 D to at or about 1,600,000 D, or an effective size of at or about 1,000,000 D to at or about 1,500,000 D.
  • the conjugate has an effecti' s. size that is at least about 8 fold greater, or at least about 10 fold greater, or at least about 12 fold greater, or at least about 15 fold greater, or at least about 18 fold greater, or at least about 20 fold greater, or at least about 25 fold greater, or at least about 28 fold greater, or at least about 30 fold greater, or at least about 40 fold greater, than the effective size of the parental antibody fragment.
  • the conjugate has an effective s.ze that is about 8 fold to about 100 fold greater, or is about 8 fold to about 80 fold greater, or is about 8 fold to about 50 fold greater, or is about 8 fold to about 40 fold greater, or is about 8 fold to about 30 fold greater, or is about 8 fold to about 28 fold greater, or is about 8 fold to about 25 fold greater, or is about 8 fold to about 20 fold greater, or is about 8 fold to about 18 fold greater, or is about 8 fold to about 15 fold greater, than the effective size of the parental antibody fragment.
  • the conjugate has an effective size that is about 12 fold to about 100 fold greater, or is about 12 fold to about 80 fold greater, or is about 12 fold to about 50 fold greater, or is about 12 fold to about 40 fold greater, or is about 12 fold to about 30 fold greater, or is about 12 fold to about 28 fold greater, or is about 12 fold to about 25 fold greater, or is about 12 fold to about 20 fold greater, or is about 12 fold to about 18 fold greater, or is about 12 fold to about 15 fold greater, than the effective size of the parental antibody fragment.
  • the conjugate has an effective size that is about 15 fold to about 100 fold greater, or is about 15 fold to about 80 fold greater, or is about 15 fold to about 50 fold greater, or is about 15 fold to about 40 fold greater, or is about 15 fold to about 30 fold greater, or is about 15 fold to about 28 fold greater, or is about 15 fold to about 25 fold greater, or is about 15 fold to about 20 fold greater, or is about 15 fold to about 18 fold greater, than the effective size ofthe parental antibody fragment.
  • the conjugate has an effective size that is about 18 fold to about 100 fold greater, or is about 18 fold to about 80 fold greater, or is about 18 fold to about 50 fold greater, or is about 18 fold to about 40 fold greater, or is about 18 fold to about 30 fold greater, or is about 18 fold to about 28 fold greater, or is about 18 fold to about 25 fold greater, or is about 18 fold to about 20 fold greater, than the effective size ofthe parental antibody fragment.
  • the conjugate has an effective size that is about 20 fold to about 100 fold greater, or is about 20 fold to about 80 fold greater, or is about 20 fold to about 50 fold greater, or is about 20 fold to about 40 fold greater, or is about 20 fold to about 30 fold greater, or is about 20 fold to about 28 fold greater, or is about 20 fold to about 25 fold greater, than the effective size ofthe parental antibody fragment.
  • the conjugate has an effective size that is about 25 fold to about 100 fold greater, or is about 25 fold to about 80 fold greater, or is about 25 fold to about 50 fold greater, or is about 25 fold to about 40 fold greater, or is about 25 fold to about 30 fold greater, or is about 25 fold to about 28 fold greater, than the effective size ofthe parental antibody fragment.
  • the conjugate has an effective size that is about 28 fold to about 100 fold greater, or is about 28 fold to about 80 fold greater, or is about 28 fold to about 50 fold greater, or is about 28 fold to about 40 fold greater, or is about 28 fold to about 30 fold greater, than the effective size of the parental antibody fragment.
  • the conjugate has un effective size that is about 30 fold to about 100 fold greater, or is about 30 fold to about 80 fold greater, or is about 30 fold to about 50 fold greater, or is about 30 fold to about 40 fold greater, than the effective size ofthe parental antibody fragment.
  • the conjugate has an effective size that is about 40 fold to about 100 fold greater, or is about 40 fold to about 80 fold greater, or is about 40 fold to about 50 fold greater, than the effective size ofthe parental antibody fragment.
  • the conjugate is an antibody fragment covalently attached to at least one polymer having an actual MW of at least about 20,000 D.
  • the conjugate is an antibody fragment covalently attached to at least one polymer having an actual MW of at least about 30,000 D.
  • the conjugate is an antibody fragment covalently attached to at least one polymer having an actual MW of at least about 40,000 D.
  • the conjugate is an antibody fragment covalently attached to at least one polymer having an actual MW that is at or about 20,000 D to at or about 300,000 D, or is at or about 30,000 D to at or about 300,000 D, or is at or about 40,000 D to at or about 300,000 D.
  • the conjugate is an antibody fragment covalently attached to at least one polymer having an actual MW that is at or about 20,000 D to at or about 100,000 D, or is at or about 30,000 D to at or about 100,000 D, or is at or about 40,000 D to at or about 100,000 D.
  • the conjugate is an antibody fragment covalently attached to at least one polymer having an actual MW that is at or about 20,000 D to at or about 70,000 D, or is at or about 30,000 D to at or about 70,000 D, or is at or about 40,000 D to at or about 70,000 D.
  • the conjugate is an antibody fragment covalently attached to at least one polymer having an actual MW that is at or about 20,000 D to at or about 50,000 D, or is at or about 30,000 D to at or about 50,000 D, or is at or about 40,000 D to at or about 50,000 D.
  • the conjugate is an antibody fragment covalently attached to at least one polymer having an actual MW that is at or about 20,000 D to at or about 40,000 D, or is at or about 30,000 D to at or about 40,000 D.
  • the conjugates of the invention can be made using any suitable technique now known or hereafter developed for derivatizing antibody fragments with polymers. It will be appreciated that the invention is not limited to conjugates utilizing any particular type of linkage between an antibody fragment and a polymer.
  • the conjugates of the invention include species wherein a polymer is covalently attached to a nonspecific site or non-specific sites on the parental antibody fragment, i.e. polymer attachment is not targeted to a particular region or a particular amino acid residue in the parental antibody fragment.
  • the coupling chemistry can, for example, utilize the free epsilon amino groups of lysine residues in the parental antibody as attachment sites for the polymer, wherein such lysine residue amino groups are randomly derivatized with polymer.
  • the conjugates of the invention include species wherein a polymer is covalently attached to a specific site or specific sites on the parental antibody fragment, i.e. polymer attachment is targeted to a particular region or a particular amino acid residue or residues in the parental antibody fragment.
  • the coupling chemistry can, for example, utilize the free sulfhydryl group of a cysteine residue not in a disulfide bridge in the parental antibody fragment.
  • one or more cysteine residue(s) is (are) engineered into a selected site or sites in the parental antibody fragment for the purpose of providing a specific attachment site or sites for polymer.
  • the polymer can be activated with any functional group that is capable of reacting specifically with the free sulfhydryl or thiol group(s) on the parental antibody, such as maleimide, sulfhydryl, thiol, triflate, tesy .ate, aziridine, exirane, and 5-pyridyl functional groups.
  • the polymer can be coupled to the parental antibody fragment using any protocol suitable for the chemistry of the coupling system selected, such as the protocols and systems described in Section (H)(1)(b) or in Section (T) of the Examples below.
  • polymer attachment is targeted to the hinge region of the parental antibody fragment.
  • the location of the hinge region varies according to the isotype of the parental antibody.
  • the hinge region of IgG, IgD and IgA isotype heavy chains is contained in a proline rich peptide sequence extending between the C H 1 and C H 2 domains.
  • a cysteine residue or residues is
  • the invention encompasses a conjugate having any molar ratio of polymer to antibody fragment that endows the conjugate with an apparent size in the desired range as taught herein.
  • the apparent size ofthe conjugate will depend in part upon the size and shape of the polymer used, the size and shape ofthe antibody fragment used, the number of polymer molecules attached to the antibody fragment, and the location of such attachment site(s) on the antibody fragment. These parameters can easily be identified and maximized to obtain the a conjugate with the desired apparent size for any type of antibody fragment, polymer and linkage system.
  • the invention encompasses a conjugate with a polymer to antibody fragment molar ratio of no more than about 10:1, or no more than about 5: 1, or no more than about 4: 1 , or no more than about 3 : 1 , or no more than about 2: 1 , or no more than 1 : 1.
  • the invention encompasses a conjugate wherein the antibody fragment is attached to about 10 or fewer polymer molecules, each polymer molecule having a molecular weight of at least about 20,000 D, or at least about 30,000 D, or at least about 40,000 D.
  • the conjugate contains an antibody fragment attached to about 5 or fewer polymer molecules, each polymer molecule having a molecular weight of at least about 20,000 D, or at least about 30,000 D, or at least about 40,000 D.
  • the conjugate contains an antibody fragment attached to about 4 or fewer polymer molecules, each polymer molecule having a molecular weight of at least about 20,000 D, or at least about 30,000 D. or at least about 40,000 D.
  • the conjugate contains an antibody fragment attached to about 3 or fewer polymer molecules, each polymer molecule having a molecular weight of at least about 20,000 D, or at least about 30,000 D, or at least about 40,000 D. In an additional embodiment, the conjugate contains an antibody fragment attached to about 2 or fewer polymer molecules, each polymer molecule having a molecular weight of at least about 20,000 D, or at least about 30,000 D, or at least about 40,000 D. Also provided herein is a conjugate containing an antibody fragment attached to a single polymer molecule having a molecular weight of at least about 20,000 D, or at least about 30,000 D, or at least about 40,000 D.
  • the invention encompasses a conjugate wherein every polymer molecule in the conjugate has a molecular weight that is at or about 20,000 D to at or about 300,000 D, or is at or about 30,000 D to at or about 300,000 D, or is at or about 40,000 D to at or about 300,000 D, and wherein the conjugate contains no more than about 10 polymer molecules, or no more than about 5 polymer molecules, or no more than about 4 polymer molecules, or no more than about 3 polymer molecules, or no more than about 2 polymer molecules, or no more than 1 polymer molecule.
  • the invention encompasses a conjugate wherein every polymer molecule in the conjugate has a molecular weight that is at or about 20,000 D to at or about 100.000 D, or is at or about 30,000 D to at or about 100,000 D, or is at or about 40,000 D to at or about 100,000 D, and wherein the conjugate contains no more than about 10 polymer molecules, or no more than about 5 polymer molecules, or no more than about 4 polymer molecules, or no more than about 3 polymer molecules, or no more than about 2 polymer molecules, or no more than 1 polymer molecule.
  • the invention encompasses a conjugate wherein every polymer molecule in the conjugate has a molecular weight that is at or about 20,000 D to at or about 70,000 D, or is at or about 30,000 D to at or about 70,000 D, or is at or about 40,000 D to at or about 70,000 D, and wherein the conjugate contains no more than about 10 polymer molecules, or no more than about 5 polymer molecules, or no more than about 4 polymer molecules, or no more than about 3 polymer molecules, or no more than about 2 polymer molecules, or no more than 1 polymer molecule.
  • the invention encompasses a conjugate wherein every polymer molecule in the conjugate has a molecular weight that is at or about 20,000 D to at or about 50,000 D, or is at or about 30,000 D to at or about 50,000 D, or is at or about 40,000 D to at or about 50,000 D, and wherein the conjugate contains no more than about 10 polymer molecules, or no more than about 5 polymer molecules, or no more than about 4 polymer molecules, or no more than about 3 polymer molecules, or no more than about 2 polymer molecules, or no more than 1 polymer molecule.
  • the invention encompasses a conjugate wherein every polymer molecule in the conjugate has a molecular weight that is at or about 20,000 D to at or about 40,000 D, or is at or about 30,000
  • the conjugate contains no more than about 10 polymer molecules, or no more than about 5 polymer molecules, or no more than about 4 polymer molecules, or no more than about 3 polymer molecules, or no more than about 2 polymer molecules, or no more than 1 polymer molecule.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', Fab'-SH, Fv, scFv and F(ab') 2 .
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein every polymer molecule in the conjugate is attached to the hinge region ofthe antibody fragment.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, every polymer molecule in the conjugate molecule is attached to the hinge region of the antibody fragment, and the conjugate contains no more than about 10 polymer molecules, or no more than about 5 polymer molecules, or no more than about 4 polymer molecules, or no more than about 3 polymer molecules, or no more than about 2 polymer molecules, or no more than 1 polymer molecule.
  • the conjugate contains a F(ab') 2 antibody fragment attached to no more than about 2 polymer molecules, wherein every polymer molecule is attached to a cysteine residue in the light or heavy chain of the antibody fragment that would ordinarily form the disulfide bridge linking the light and heavy chains, wherein the disulfide bridge is avoided by substituting another amino acid, such as serine, for the corresponding cysteine residue in the opposite chain.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 polymer molecule and the polymer is coupled to a cysteine residue in the light or heavy chain of the antibody fragment that would ordinarily form the disulfide bridge linking the light and heavy chains, wherein the disulfide bridge is avoided by substituting another amino acid, such as serine, for the corresponding cysteine residue in the opposite chain.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, every polymer molecule in the conjugate is at least about 20,000 D in molecular weight, or at least about 30,000 in molecular weight, or at least about 40,000 D in molecular weight, every polymer molecule in the conjugate is attached to the hinge region of the antibody fragment, and the conjugate contains no more than about 10 polymer molecules, or no more than about 5 polymer molecules, or no more than about 4 polymer molecules, or no more than about 3 polymer molecules, or no more than about 2 polymer molecules, or no more than 1 polymer molecule.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, every polymer molecule in the conjugate is at or about 20,000 D to at or about 300,000 D in molecular weight, or is at or about 30,000 D to at or about 300,000 D in molecular weight, or is at or about 40,000 D to at or about 300,000 D in molecular weight, every polymer molecule in the conjugate is attached to the hinge region of the antibody fragment, and the conjugate contains no more than about 10 polymer molecules, or no more than about 5 polymer molecules, or no more than about 4 polymer molecules, or no more than about 3 polymer molecules, or no more than about 2 polymer molecules, or no more than 1 polymer molecule.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, every polymer molecule in the conjugate is at or about 20,000 D to at or about 100,000 D in molecular weight, or is at or about 30,000 D to at or about 100,000 D in molecular weight, or is at or about 40,000 D to at or about 100,000 D in molecular weight, every polymer molecule in the conjugate is attached to the hinge region of the antibody fragment, and the conjugate contains no more than about 10 polymer molecules, or no more than about 5 polymer molecules, or no more than about 4 polymer molecules, or no more than about 3 polymer molecules, or no more than about 2 polymer molecules, or no more than 1 polymer molecule.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, every polymer molecule in the conjugate is at or about 20,000 D to at or about 70,000 D in molecular weight, or is at or about 30,000 D to at or about 70,000 D in molecular weight, or is at or about 40,000 D to at or about 70,000 D in molecular weight, every polymer molecule in the conjugate is attached to the hinge region of the antibody fragment, and the conjugate contains no more than about 10 polymer molecules, or no more than about 5 polymer molecules, or no more than about 4 polymer molecules, or no more than about 3 polymer molecules, or no more than about 2 polymer molecules, or no more than 1 polymer molecule.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, every polymer molecule in the conjugate is at or about 20,000 D to at or about 50,000 D in molecular weight, or is at or about 30,000 D to at or about 50,000 D in molecular weight, or is at or about 40,000 D to at or about 50,000 D in molecular weight, every polymer molecule in the conjugate is attached to the hinge region of the antibody fragment, and the conjugate contains no more than about 10 polymer molecules, or no more than about 5 polymer molecules, or no more than about 4 polymer molecules, or no more than about 3 polymer molecules, or no more than about 2 polymer molecules, or no more than 1 polymer molecule.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, every polymer molecule in the conjugate is at or about 20,000 D to at or about 40,000 D in molecular weight, or is at or about 30,000 D to at or about 40,000 D in molecular weight, every polymer molecule in the conjugate is attached to the hinge region of the antibody fragment, and the conjugate contains no more than about 10 polymer molecules, or no more than about 5 polymer molecules, or no more than about 4 polymer molecules, or no more than about 3 polymer molecules, or no more than about 2 polymer molecules, or no more than 1 polymer molecule.
  • the conjugate contains a F(ab') 2 antibody fragment attached to no more than about 2 polymer molecules, wherein every polymer molecule in the conjugate is at least about 20,000 D in molecular weight, or at least about 30,000 D in molecular weight, or at least about 40,000 D in molecular weight, and wherein every polymer molecule in the conjugate is attached to a cysteine residue in the light or heavy chain of the antibody fragment that would ordinarily form the disulfide bridge linking the light and heavy chains, wherein the disulfide bridge is avoided by substituting another amino acid, such as serine, for the corresponding cysteine residue in the opposite chain.
  • every polymer molecule in the conjugate is at least about 20,000 D in molecular weight, or at least about 30,000 D in molecular weight, or at least about 40,000 D in molecular weight
  • every polymer molecule in the conjugate is attached to a cysteine residue in the light or heavy chain of the antibody fragment that would ordinarily form the disulfide bridge linking the
  • the conjugate contains a F(ab') 2 antibody fragment attached to no more than about 2 polymer molecules, wherein every polymer molecule in the conjugate is at or about 20,000 D to at or about 300,000 D in molecular weight, or is at or about 30,000 D to at or about 300,000 D in molecular weight, or is at or about 40,000 D to at or about 300,000 D in molecular weight, and wherein every polymer molecule in the conjugate is attached to a cysteine residue in the light or heavy chain of the antibody fragment that would ordinarily form the disulfide bridge linking the light and heavy chains, wherein the disulfide bridge is avoided by substituting another amino acid, such as serine, for the corresponding cysteine residue in the opposite chain.
  • every polymer molecule in the conjugate is at or about 20,000 D to at or about 300,000 D in molecular weight, or is at or about 30,000 D to at or about 300,000 D in molecular weight, or is at or about 40,000 D to at or about 300
  • the conjugate contains a F(ab') 2 antibody fragment attached to no more than about 2 polymer molecules, wherein every polymer molecule in the conjugate is at or about 20,000 D to at or about 100,000 D in molecular weight, or is at or about 30,000 D to at or about 100,000 D in molecular weight, or is at or about 40,000 D to at or about 100,000 D in molecular weight, and wherein every polymer molecule in the conjugate is attached to a cysteine residue in the light or heavy chain of the antibody fragment that would ordinarily form the disulfide bridge linking the light and heavy chains, wherein the disulfide bridge is avoided by substituting another amino acid, such as serine, for the corresponding cysteine residue in the opposite chain.
  • every polymer molecule in the conjugate is at or about 20,000 D to at or about 100,000 D in molecular weight, or is at or about 30,000 D to at or about 100,000 D in molecular weight, or is at or about 40,000 D to at or about 100,000 D in molecular weight
  • the conjugate contains a F(ab') 2 antibody fragment attached to no more than about 2 polymer molecules, wherein every polymer molecule in the conjugate is at or about 20,000 D to at or about 70,000 D in molecular weight, or is at or about 30,000 D to at or about 70,000 D in molecular weight, or is at or about 40,000 D to at or about 70,000 D in molecular weight, and wherein every polymer molecule in the conjugate is attached to a cysteine residue in the light or heavy chain of the antibody fragment that would ordinarily form the disulfide bridge linking the light and heavy chains, wherein the disulfide bridge is avoided by substituting another amino acid, such as serine, for the corresponding cysteine residue in the opposite chain.
  • every polymer molecule in the conjugate is at or about 20,000 D to at or about 70,000 D in molecular weight, or is at or about 30,000 D to at or about 70,000 D in molecular weight, or is at or about 40,000 D to at or about 70,000 D in mole
  • the conjugate contains a F(ab') 2 antibody fragment attached to no more than about 2 polymer molecules, wherein every polymer molecule in the conjugate is at or about 20,000 D to at or about 50,000 D in molecular weight, or is at or about 30,000 D to at or about 50,000 D in molecular weight, or is at or about 40,000 D to at or about 50,000 D in molecular weight, and wherein every polymer molecule in the conjugate is attached to a cysteine residue in the light or heavy chain of the antibody fragment that would ordinarily form the disulfide bridge linking the light and heavy chains, wherein the disulfide bridge is avoided by substituting another amino acid, such as serine, for the corresponding cysteine residue in the opposite chain.
  • every polymer molecule in the conjugate is at or about 20,000 D to at or about 50,000 D in molecular weight, or is at or about 30,000 D to at or about 50,000 D in molecular weight, or is at or about 40,000 D to at or about 50,000 D in mole
  • the conjugate contains a F(ab') 2 antibody fragment attached to no more than about 2 polymer molecules, wherein every polymer molecule in the conjugate is at or about 20,000 D to at or about 40,000 D in molecular weight, or is at or about 30,000 D to at or about 40,000 D in molecular weight, and wherein every polymer molecule in the conjugate is attached to a cysteine residue in the light or heavy chain of the antibody fragment that would ordinarily form the disulfide bridge linking the light and heavy chains, wherein the disulfide bridge is avoided by substituting another amino acid, such as serine, for the corresponding cysteine residue in the opposite chain.
  • every polymer molecule in the conjugate is at or about 20,000 D to at or about 40,000 D in molecular weight, or is at or about 30,000 D to at or about 40,000 D in molecular weight
  • every polymer molecule in the conjugate is attached to a cysteine residue in the light or heavy chain of the antibody fragment that would ordinarily form the disulf
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 polymer molecule, wherein the polymer molecule is at least about 20,000 D in molecular weight, or at least about 30,000 D in molecular weight, or at least about 40,000 D in molecular weight, wherein the polymer molecule is attached to a cysteine residue in the light or heavy chain ofthe antibody fragment that would ordinarily form the disulfide bridge linking the light and heavy chains, wherein the disulfide bridge is avoided by substituting another amino acid, such as serine, for the corresponding cysteine residue in the opposite chain.
  • an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 polymer molecule, wherein the polymer molecule is at least about 20,000 D in molecular weight, or at least about 30,000 D in molecular weight, or at least about 40,000 D
  • the conjugate contains an antibody fragment select.-d from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 polymer molecule, wherein the polymer molecule is at or about 20,000 D to at or about 300,000 D in molecular weight, or is at or about 30,000 D to at or about 300,000 D in molecular weight, or is at or about 40,000 D to at or about 300,000 D in molecular weight, wherein the polymer molecule is attached to a cysteine residue in the light or heavy chain of the antibody fragment that would ordinarily form the disulfide bridge linking the light and heavy chains, wherein the disulfide bridge is avoided by substituting another amino acid, such as serine, for the corresponding cysteine residue in the opposite chain.
  • the polymer molecule is at or about 20,000 D to at or about 300,000 D in molecular weight, or is at or about 30,000 D to at or about 300,000 D in molecular weight, or is at or
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 polymer molecule, wherein the polymer molecule is at or about 20,000 D to at or about 100,000 D in molecular weight, or is at or about 30,000 D to at or about 100,000 D in molecular weight, or is at or about 40,000 D to at or about 100,000 D in molecular weight, wherein the polymer molecule is attached to a cysteine residue in the light or heavy chain of the antibody fragment that would ordinarily form the disulfide bridge linking the light and heavy chains, wherein the disulfide bridge is avoided by substituting another amino acid, such as serine, for the corresponding cysteine residue in the opposite chain.
  • an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 polymer molecule, wherein the polymer molecule is at or about 20,000 D to at or about 100,000 D
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 polymer molecule, wherein the polymer molecule is at or about 20,000 D to at or about 70,000 D in molecular weight, or is at or about 30,000 D to at or about 70,000 D in molecular weight, or is at or about 40,000 D to at or about 70,000 D in molecular weight, wherein the polymer molecule is attached to a cysteine residue in the light or heavy chain of the antibody fragment that would ordinarily form the disulfide bridge linking the light and heavy chains, wherein the disulfide bridge is avoided by substituting another amino acid, such as serine, for the corresponding cysteine residue in the opposite chain.
  • an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 polymer molecule, wherein the polymer molecule is at or about 20,000 D to at or
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 polymer molecule, wherein the polymer molecule is at or about 20,000 D to at or about 50,000 D in molecular weight, or is at or about 30,000 D to at or about 50,000 D in molecular weight, or is at or about 40,000 D to at or about 50,000 D in molecular weight, wherein the polymer molecule is attached to a cysteine residue in the light or heavy chain of the antibody fragment that would ordinarily form the disulfide bridge linking the light and heavy chains, wherein the disulfide bridge is avoided by substituting another amino acid, such as serine, for the corresponding cysteine residue in the opposite chain.
  • an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 polymer molecule, wherein the polymer molecule is at or about 20,000 D to at or
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 polymer molecule, wherein the polymer molecule is at or about 20,000 D to at or about 40,000 D in molecular weight, or is at or about 30,000 D to at or about 40,000 D in molecular weight, wherein the polymer molecule is attached to a cysteine residue in the light or heavy chain of the antibody fragment that would ordinarily form the disulfide bridge linking the light and heavy chains, wherein the disulfide bridge is avoided by substituting another amino acid, such as serine, for the corresponding cysteine residue in the opposite chain.
  • an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 polymer molecule, wherein the polymer molecule is at or about 20,000 D to at or about 40,000 D in molecular weight, or is at or about 30,000 D to at or
  • the conjugate contains an antibody fragment '.elected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 polymer molecule, wherein the polymer molecule is at least about 20,000 D in molecular weight, or at least about 30,000 D in molecular weight, or at least about 40,000 D in molecular weight, and wherein the polymer molecule is attached to the hinge region ofthe antibody fragment.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 polymer molecule, wherein the polymer molecule is at or about 20,000 D to at or about 300,000 D in molecular weight, or is at or about 30,000 D to at or about 300,000 D in molecular weight, or is at or about 40,000 D to at or about 300,000 D in molecular weight, and wherein the polymer molecule is attached to the hinge region of the antibody fragment.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 polymer molecule, wherein the polymer molecule is at or about 20,000 D to at or about 100,000 D in molecular weight, or is at or about 30,000 D to at or about 100,000 D in molecular weight, or is at or about 40,000 D to at or about 100,000 D in molecular weight, and wherein the polymer molecule is attached to the hinge region ofthe antibody fragment.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 polymer molecule, wherein the polymer molecule is at or about 20,000 D to at or about 70,000 D in molecular weight, or is at or about 30,000 D to at or about 70,000 D in molecular weight, or is at or about 40,000 D to at or about 70,000 D in molecular weight, and wherein the polymer molecule is attached to the hinge region of the antibody fragment.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 polymer molecule, wherein the polymer molecule is at or about 20,000 D to at or about 50,000 D in molecular weight, or is at or about 30,000 D to at or about 50,000 D in molecular weight, or is at or about 40,000 D to at or about 50,000 D in molecular weight, and wherein the polymer molecule is attached to the hinge region of the antibody fragment.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 polymer molecule, wherein the polymer molecule is at or about 20,000 D to at or about 40,000 D in molecular weight, or is at or about 30,000 D to at or about 40,000 D in molecular weight, and wherein the polymer molecule is attached to the hinge region ofthe antibody fragment.
  • polyethylene glycol (PEG) polymers are preferred for use herein.
  • the conjugate is an antibody fragment covalently attached to at least one PEG having an actual M W of at least about 20,000 D.
  • the conjugate is an antibody fragment covalentiy attached to at least one PEG having an actual M W of at least about 30,000 D.
  • the conjugate is an antibody fragment covalently attached to at least one PEG having an actual MW of at least about 40,000 D.
  • the conjugate is an antibody fragment covalently attached to at least one PEG having an actual MW that is at or about 20,000 D to at or about 300,000 D, or is at or about 30,000 D to at or about 300,000 D, or is at or about 40,000 D to at or about 300,000 D.
  • the conjugate is an antibody fragment covalently attached to at least one PEG having an actual MW that is at or about 20,000 D to at or about 100,000 D, or is at or about 30,000 D to at or about 100,000 D, or is at or about 40,000 D to at or about 100,000 D.
  • the conjugate is an antibody fragment covalently attached to at least one PEG having an actual MW that is at or about 20,000 D to at or about 70,000 D, or is at or about 30,000 D to at or about 70,000 D, or is at or about 40,000 D to at or about 70,000 D.
  • the conjugate is an antibody fragment covalently attached to at least one PEG having an actual MW that is at or about 20,000 D to at or about 50,000 D, or is at or about 30,000 D to at or about 50,000 D, or is at or about 40,000 D to at or about 50,000 D.
  • the conjugate is an antibody fragment covalently attached to at least one PEG having an actual MW that is at or about 20,000 D to at or about 40,000 D, or is at or about 30,000 D to at or about 40,000 D.
  • the invention encompasses a conjugate with a PEG to antibody fragment molar ratio of no more than about 10:1, or no more than about 5:1, or no more than about 4: 1, or no more than about 3: 1, or no more than about 2: 1 , or no more than 1 : 1.
  • the invention encompasses a conjugate wherein the antibody fragment is attached to about 10 or fewer PEG molecules, each PEG molecule having a molecular weight of at least about 20,000 D, or at least about 30,000 D, or at least about 40,000 D.
  • the conjugate contains an antibody fragment attached to about 5 or fewer PEG molecules, each PEG molecule having a molecular weight of at least about 20,000 D, or at least about 30,000 D, or at least about 40,000 D.
  • the conjugate contains an antibody fragment attached to about 4 or fewer PEG molecules, each PEG molecule having a molecular weight of at least about 20,000 D, or at least about 30,000 D, or at least about 40,000 D.
  • the conjugate contains an antibody fragment attached to about 3 or fewer PEG molecules, each PEG molecule having a molecular weight of at least about 20,000 D, or at least about 30,000 D, or at least about 40,000 D. In an additional embodiment, the conjugate contains an antibody fragment attached to about 2 or fewer PEG molecules, each PEG molecule having a molecular weight of at least about 20,000 D, or at least about 30,000 D, or at least about 40,000 D. Also provided herein is a conjugate containing an antibody fragment attached to a single PEG molecule having a molecular weight of at least about 20,000 D, or at least about 30,000 D, or at least about 40,000 D.
  • the invention encompasses a conjugate wherein the antibody fragment is derivatized with PEG, wherein every PEG molecule in the conjugate is at or about 20,000 D to at or about 300,000 D in molecular weight, or is at or about 30,000 D to at or about 300,000 D in molecular weight, or is at or about 40,000 D to at or about 300,000 D in molecular weight, and wherein me conjugate contains no more than about 10 PEG molecules, or no more than about 5 PEG molecules, or no more than about 4 PEG molecules, or no more than about 3 PEG molecules, or no more than about 2 PEG molecules, or no more than 1 PEG molecule.
  • the invention encompasses a conjugate wherein the antibody fragment is derivatized with PEG, wherein every PEG molecule in the conjugate is at or about 20,000 D to at or about 100,000 D in molecular weight, or is at or about 30,000 D to at or about 100,000 D ir_ molecular weight, or is at or about 40,000 D to at or about 100,000 D in molecular weight, and wherein the conjugate contains no more than about 10 PEG molecules, or no more than about 5 PEG molecules, or o more than about 4 PEG molecules, or no more than about 3 PEG molecules, or no more than about 2 PEG molecules, or no more than 1 PEG molecule.
  • the invention encompasses a conjugate wherein the antibody fragment is derivatized with PEG, wherein every PEG molecule in the conjugate is at or about 20,000 D to at or about 70,000 D in molecular weight, or is at or about 30,000 D to at or about 70,000 D in molecular weight, or is at or about
  • PEG molecules or no more than about 5 PEG molecules, or no more than about 4 PEG molecules, or no more than about 3 PEG molecules, or no more than about 2 PEG molecules, or no more than 1 PEG molecule.
  • the invention encompasses a conjugate wherein the antibody fragment is derivatized with PEG, wherein every PEG molecule in the conjugate is at or about 20,000 D to at or about 50,000 D in molecular weight, or is at or about 30,000 D to at or about 50,000 D in molecular weight, or is at or about 40,000 D to at or about 50,000 D in molecular weight, and wherein the conjugate contains no more than about 10 PEG molecules, or no more than about 5 PEG molecules, or no more than about 4 PEG molecules, or no more than about 3 PEG molecules, or no more than about 2 PEG molecules, or no more than 1 PEG molecule.
  • the invention encompasses a conjugate wherein the antibody fragment is derivatized with PEG, wherein every PEG molecule in the conjugate is at or about 20,000 D to at or about 40,000 D in molecular weight, or is at or about 30,000 D to at or about 40,000 D in molecular weight, and wherein the conjugate contains no more than about 10 PEG molecules, or no more than about 5 PEG molecules, or no more than about 4 PEG molecules, or no more than about 3 PEG molecules, or no more than about 2 PEG molecules, or no more than 1 PEG molecule.
  • the invention encompasses a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', Fab'-SH and F(ab') 2 , wherein the antibody fragment is attached to about 10 or fewer PEG molecules, each PEG molecule having a molecular weight of at least about 20,000 D, or at least about 30,000 D, or at least about 40,000 D.
  • the foregoing conjugate contains an antibody fragment attached to about 5 or fewer PEG molecules, each PEG molecule having a molecular weight of at least about 20,000 D, or at least about 30,000 D, or at least about 40,000 D.
  • the foregoing conjugate contains an antibody fragment attached to about 4 or fewer
  • the foregoing conjugate contains an antibody fragment attached to about 3 or fewer PEG molecules, each PEG molecule having a molecular weight of at least about 20,000 D, or at least about 30,000 D, or at least about 40,000 D.
  • the foregoing conjugate contains an antibody fragment attached to about 2 or fewer PEG molecules, each PEG molecule having a molecular weight of at least about 20,000 D, or at least about 30,000 D, or at least about 40,000 D.
  • Also provided herein is the foregoing conjugate that contains an antibody fragment attached to a single PEG molecule having a molecular weight of at least about 20,000 D, or at least about 30,000 D, or at least about 40,000 D.
  • the invention encompasses a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', Fab'-SH and F(ab') 2 .
  • the antibody fragment is derivatized with PEG, wherein every PEG molecule in the conjugate is at or about 20,000 D to at or about 300,000 D in molecular weight, or is at or about 30,000 D to at or about 300,000 D in molecular weight, or is at or about 40,000 D to at or about 300,000 D in molecular weight, and wherein the conjugate contains no more than about 10 PEG molecules, or no more than about 5 PEG molecules, or no more than about 4 PEG molecules, or no more than about 3 PEG molecules, or no more than about 2 PEG molecules, or no more than 1 PEG molecule.
  • the invention encompasses a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', Fab'-SH and F(ab') 2 , wherein the antibody fragment is derivatized with PEG, wherein every PEG molecule in the conjugate is at or about 20,000 D to at or about 100,000 D in molecular weight, or is at or about 30,000 D to at or about 100,000 D in molecular weight, or is at or about 40,000 D to at or about 100,000 D in molecular weight, and wherein the conjugate contains no more than about 10 PEG molecules, or no more than about 5 PEG molecules, or no more than about 4 PEG molecules, or no more than about 3 PEG molecules, or no more than about 2 PEG molecules, or no more than 1 PEG molecule.
  • the invention encompasses a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', Fab'-SH and F(ab') , wherein the antibody fragment is derivatized with PEG, wherein every PEG molecule in the conjugate is at or about 20,000 D to at or about 70,000 D in molecular weight, or is at or about 30,000 D to at or about 70,000 D in molecular weight, or is at or about 40,000 D to at or about 70,000 D in molecular weight, and wherein the conjugate contains no more than about 10 PEG molecules, or no more than about 5 PEG molecules, or no more than about 4 PEG molecules, or no more than about 3 PEG molecules, or no more than about 2 PEG molecules, or no more than 1 PEG molecule.
  • the invention encompasses a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', Fab'-SH and F(ab') 2 , wherein the antibody fragment is derivatized with PEG, wherein every PEG molecule in the conjugate is at or about 20,000 D to at or about 50,000 D in molecular weight, or is at or about 30,000 D to at or about 50,000 D in molecular weight, or is at or about 40,000 D to at or about 50,000 D in molecular weight, and wherein the conjugate contains no more than about 10 PEG molecules, or no more than about 5 PEG molecules, or no more than about 4 PEG molecules, or no more than about 3 PEG molecules, or no more than about 2 PEG molecules, or no more than 1 PEG molecule.
  • the invention encompasses a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', Fab'-SH and F(ab') 2 , wherein the antibody fragment is derivatized with PEG, wherein every PEG molecule in the conjugate is at or about 20,000 D to at or about 40,000 D in molecular weight, or is at or about 30,000 D to at or about 40,000 D in molecular weight, and wherein the conjugate contains no more than about 10 PEG molecules, or no more than about 5 PEG molecules, or no more than about 4 PEG molecules, or no more than about 3 PEG molecules, or no more than about 2 PEG molecules, or no more than 1 PEG molecule.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is derivatized with PEG having a molecular weight of at least about 20,000D, or at least aoout 30,000D, or at least about 40,000D, and wherein every PEG molecule in the conjugate is attached to the hinge region ofthe antibody fragment.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is derivatized with PEG having a molecular weight that is at or about 20,000 D to about 300,000 D, or is at or about 30,000 D to at or about 300,000 D, or is at or about 40,000 D to at or about 300,000 D, and wherein every PEG molecule in the conjugate is attached to the hinge region ofthe antibody fragment.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is derivatized with PEG having a molecular weight that is at or about 20,000 D to about 100,000 D, or is at or about 30,000 D to at or about 100,000 D, or is at or about 40,000 D to at or about 100.000 D, and wherein every PEG molecule in the conjugate is attached to the hinge region ofthe antibody fragment.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is derivatized with PEG having a molecular weight that is at or about 20,000 D to about 70,000 D, or is at or about 30,000 D to at or about 70,000 D, or is at or about 40,000 D to at or about 70,000 D. and wherein every PEG molecule in the conjugate is attached to the hinge region ofthe antibody fragment.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is derivatized with PEG having a molecular weight that is at or about 20,000 D to about 50,000 D, or is at or about 30,000 D to at or about 50,000 D, or is at or about 40,000 D to at or about 50,000 D, and wherein every PEG molecule in the conjugate is attached to the hinge region ofthe antibody fragment.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is derivatized with PEG having a molecular weight that is at or about 20,000 D to about 40,000 D, or is at or about 30,000 D to at or about 40,000 D, and wherein every PEG molecule in the conjugate is attached to the hinge region of the antibody fragment.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is derivatized with PEG, wherein every PEG molecule in the conjugate is at least about 20,000D in molecular weight, or at least about 30,000D in molecular weight, or at least about 40,000D in molecular weight, wherein every PEG molecule in the conjugate molecule is attached to the hinge region of the antibody fragment, and wherein the conjugate contains no more than about 10 PEG molecules, or no more than about 5 PEG molecules, or no more than about 4 PEG molecules, or no more than about 3 PEG molecules, or no more than about 2 PEG molecules, or no more than 1 PEG molecule.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is derivatized with PEG, wherein every PEG molecule in the conjugate is at or about 20,000 D to at or about 300,000 D in molecular weight, or is at or about 30,000 D to at or about 300,000 D in molecular weight, or is at or about 40,000 D to at or about 300,000 D in molecular weight, wherein every _'EG molecule in the conjugate molecule is attached to the hinge region of the antibody fragment, and wherein the conjugate contains no more than about 10 PEG molecules, or no more than about 5 PEG molecules, or no more than about 4 PEG molecules, or no more than about 3 PEG molecules, or no more than about 2 PEG molecules, or no more than 1 PEG molecule.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is derivatized with PEG, wherein every PEG molecule in the conjugate is at or about 20,000 D to at or about 100,000 D in molecular weight, or is at or about 30,000 D to at or about 100,000 D in molecular weight, or is at or about 40,000 D to at or about 100,000 D in molecular weight, wherein every PEG molecule in the conjugate molecule is attached to the hinge region of the antibody fragment, and wherein the conjugate contains no more than about 10 PEG molecules, or no more than about 5 PEG molecules, or no more than about 4 PEG molecules, or no more than about 3 PEG molecules, or no more than about 2 PEG molecules, or no more than 1 PEG molecule.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is derivatized with PEG, wherein every PEG molecule in the conjugate is at or about 20,000 D to at or about 70,000 D in molecular weight, or is at or about 30,000 D to at or about 70,000 D in molecular weight, or is at or about 40,000 D to at or about 70,000 D in molecular weight, wherein every PEG molecule in the conjugate molecule is attached to the hinge region ofthe antibody fragment, and wherein the conjugate contains no more than about 10 PEG molecules, or no more than about 5 PEG molecules, or no more than about 4 PEG molecules, or no more than about 3 PEG molecules, or no more than about 2 PEG molecules, or no more than 1 PEG molecule.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is derivatized with PEG, wherein every PEG molecule in the conjugate is at or about 20,000 D to at or about 50,000 D in molecular weight, or is at or about 30,000 D to at or about 50,000 D in molecular weight, or is at or about 40,000 D to at or about 50,000 D in molecular weight, wherein every PEG molecule in the conjugate molecule is attached to the hinge region of the antibody fragment, and wherein the conjugate contains no more than about 10 PEG molecules, or no more than about 5 PEG molecules, or no more than about 4 PEG molecules, or no more than about 3 PEG molecules, or no more than about 2 PEG molecules, or no more than 1 PEG molecule.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is derivatized with PEG, wherein every PEG molecule in the conjugate is at or about 20,000 D to at or about 40,000 D in molecular weight, or is at or about 30,000 D to at or about 40,000 D in molecular weight, wherein every PEG molecule in the conjugate molecule is attached to the hinge region of the antibody fragment, and wherein the conjugate contains no more than about 10 PEG molecules, or no more than about 5 PEG molecules, or no more than about 4 PEG molecules, or no more than about 3 PEG molecules, or no more than about 2 PEG molecules, or no more than 1 PEG molecule.
  • the conjugate contains a F(ab') 2 antibody fragment derivatized with PEG, wherein every PEG molecule in the conjugate is at least about 20,000D in molecular weight, or at least about 30,000D in molecular weight, or at least about 40.000D in molecular weight, wherein the antibody fragment is attached to no more than about 2 PEG molecules, and wherein every PEG molecule is attached to a cysteine residue in the light or heavy chain of the antibody fragment that would ordinarily form the disulfide bridge linking the light and heavy chains, wherein the disulfide bridge is avoided by substituting another amino acid, such as serine, for the corresponding cysteine residue in the opposite chain.
  • every PEG molecule in the conjugate is at least about 20,000D in molecular weight, or at least about 30,000D in molecular weight, or at least about 40.000D in molecular weight
  • the antibody fragment is attached to no more than about 2 PEG molecules
  • every PEG molecule is attached to a cysteine residue in the
  • the conjugate contains a F(ab') ? antibody fragment derivatized with
  • every PEG molecule in the conjugate is at or about 20,000 D to at or about 300,000 D in molecular weight, or is at or about 30,000 D to at or about 300,000 D in molecular weight, or is at or about 40,000 D to at or about 300,000 D in molecular weight, wherein the antibody fragment is attached to no more than about 2 PEG molecules, and wherein every PEG molecule is attached to a cysteine residue in the light or heavy chain of the antibody fragment that would ordinarily form the disulfide bridge linking the light and heavy chains, wherein the disulfide bridge is avoided by substituting another amino acid, such as serine, for the corresponding cysteine residue in the opposite chain.
  • another amino acid such as serine
  • the conjugate contains a F(ab') antibody fragment derivatized with
  • every PEG molecule in the conjugate is at or about 20,000 D to at or about 100,000 D in molecular weight, or is at or about 30,000 D to at or about 100,000 D in molecular weight, or is at or about 40,000 D to at or about 100,000 D in molecular weight, wherein the antibody fragment is attached to no more than about 2 PEG molecules, and wherein every PEG molecule is attached to a cysteine residue in the light or heavy chain of the antibody fragment that would ordinarily form the disulfide bridge linking the light and heavy chains, wherein the disulfide bridge is avoided by substituting another amino acid, such as serine, for the corresponding cysteine residue in the opposite chain.
  • another amino acid such as serine
  • the conjugate contains a F(ab') 2 antibody fragment derivatized with
  • every PEG molecule in the conjugate is at or about 20,000 D to at or about 70,000 D in molecular weight, or is at or about 30,000 D to at or about 70.000 D in molecular weight, or is at or about 40,000 D to at or about 70,000 D in molecular weight, wherein the antibody fragment is attached to no more than about 2 PEG molecules, and wherein every PEG molecule is attached to a cysteine residue in the light or heavy chain of the antibody fragment that would ordinarily form the disulfide bridge linking the light and heavy chains, wherein the disulfide bridge is avoided by substituting another amino acid, such as serine, for the corresponding cysteine residue in the opposite chain.
  • another amino acid such as serine
  • the conjugate contains a F(ab') 2 antibody fragment derivatized with PEG, wherein every PEG molecule in the conjugate is at or about 20,000 D to at or about 50,000 D in molecular weight, or is at or about 30,000 D to at or about 50,000 D in molecular weight, or is at or about 40,000 D to at or about 50,000 D in molecular weight, wherein the antibody fragment is attached to no more than about 2 PEG molecules, and wherein every PEG molecule is attached to a cysteine residue in the light or heavy chain of the antibody fragment that would ordinarily form the disulfide bridge linking the light and heavy chains, wherein the disulfide bridge is avoided by substituting another amino acid, such as serine, for the corresponding cysteine residue in the opposite chain.
  • every PEG molecule in the conjugate is at or about 20,000 D to at or about 50,000 D in molecular weight, or is at or about 30,000 D to at or about 50,000 D in molecular weight, or is at or about 40,000 D
  • the conjugate contains a F(ab') 2 antibody fragment derivatized with
  • every PEG molecule in the conjugate is at or about 20,000 D to at or about 40,000 D in molecular weight, or is at or about 30,000 D to at or about 40,000 D in molecular weight, wherein the antibody fragment is attached to no more than about 2 PEG molecules, and wherein every PEG molecule is attached to a cysteine residue in the light or heavy chain of the antibody fragment that would ordinarily form the disulfide bridge linking the light and heavy chains, wherein the disulfide bridge is avoided by substituting another amino acid, such as serine, for the corresponding cysteine residue in the opposite chain.
  • another amino acid such as serine
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is derivatized with PEG, wherein every PEG molecule in the conjugate is at least about 20,000 D in molecular weight, or at least about 30,000 in molecular weight, or at least about 40,000 D in molecular weight, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is attached to a cysteine residue in the light or heavy chain of the antibody fragment that would ordinarily form the disulfide bridge linking the light and heavy chains, wherein the disulfide bridge is avoided by substituting another amino acid, such as serine, for the corresponding cysteine residue in the opposite chain.
  • PEG amino acid
  • the conjugate contains an antibody fragment selected from the group consisting of Fab. Fab', and Fab'-SH. wherein the antibody fragment is derivatized with PEG, wherein every PEG molecule in the conjugate is at or about 20,000 D to at or about 300,000 D in molecular weight, or is at or about 30,000 D to at or about 300,000 D in molecular weight, or is at or about 40,000 D to at or about 300,000 D in molecular weight, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is attached to a cysteine residue in the light or heavy chain of the antibody fragment that would ordinarily form the disulfide bridge linking the light and heavy chains, wherein the disulfide bridge is avoided by substituting another amino acid, such as serine, for the corresponding cysteine residue in the opposite chain.
  • PEG amino acid
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is derivatized with PEG, wherein every PEG molecule in the conjugate is at or about 20,000 D to at or about 100,000 D in molecular weight, or is at or about 30,000 D to at or about 100,000 D in molecular weight, or is at or about 40,000 D to at or about 100,000 D in molecular weight, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is attached to a cysteine residue in the light or heavy chain of the antibody fragment that would ordinarily form the disulfide bridge linking the light and heavy chains, wherein the disulfide bridge is avoided by substituting another amino acid, such as serine, for the corresponding cysteine residue in the opposite chain.
  • PEG an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is derivatized with PEG, wherein every PEG molecule in .he conjugate is at or about 20,000 D to at or about 70,000 D in molecular weight, or is at or about 30,000 D to at or about 70,000 D in molecular weight, or is at or about 40,000 D to at or about 70,000 D in molecular weight, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is attached to a cysteine residue in the light or heavy chain of the antibody fragment that would ordinarily form the disulfide bridge linking the light and heavy chains, wherein the disulfide bridge is avoided by substituting another amino acid, such as serine, for the corresponding cysteine residue in the opposite chain.
  • the conjugate contains an antibody fragment selected from he group consisting oi ' Fab, Fab', and Fab'-SH, wherein the antibody fragment is derivatized with PEG, wherein every PEG molecule in the conjugate is at or about 20,000 D to at or about 50,000 D in molecular weight, or is at or about 30,000 D to at or about 50,000 D in molecular weight, or is at or about 40,000 D to at or about 50,000 D in molecular weight, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is attached to a cysteine residue in the light or heavy chain of the antibody fragment that would ordinarily form the disulfide bridge linking the light and heavy chains, wherein the disulfide bridge is avoided by substituting another amino acid, such as serine, for the corresponding cysteine residue in the opposite chain.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is derivatized with PEG, wherein every PEG molecule in the conjugate is at or about 20,000 D to at or about 40,000 D in molecular weight, or is at or about 30,000 D to at or about 40,000 D in molecular weight, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is attached to a cysteine residue in the light or heavy chain of the antibody fragment that would ordinarily form the disulfide bridge linking the light and heavy chains, wherein the disulfide bridge is avoided by substituting another amino acid, such as serine, for the corresponding cysteine residue in the opposite chain.
  • PEG amino acid
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is branched and at least about 40,000 D in molecular weight.
  • the inventors discovered that the foregoing conjugate exhibits a serum half-life, MRT and serum clearance rate approaching that of full length antibody as shown in Example X below.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is branched and has a molecular weight that is at or about 40,000 D to at or about 300,000 D.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is branched and has a molecular weight that is at or about 40,000 D to at or about 100,000 D.
  • the conjugate contains an antibody fragment selected frc.n the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is branched and has a molecular weight that is at or about 40,000 D to at or about 70,000 D.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 Ph ⁇ molecule, and wherein the PEG molecule is branched and has a molecular weight that is at or about 40,000 D to at or about 50,000 D.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is branched and at least 40,000D in molecular weight, and the PEG molecule is attached to the hinge region ofthe antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is branched and has a molecular weight that is at or about 40,000 D to at or about 300,000 D, and the PEG molecule is attached to the hinge region ofthe antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is branched and has a molecular weight that is at or about 40,000 D to at or about 100,000 D, and the PEG molecule is attached to the hinge region ofthe antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is branched and has a molecular weight that is at or about 40,000 D to at or about 70,000 D, and the PEG molecule is attached to the hinge region of the antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is branched and has a molecular weight that is at or about 40,000 D to at or about 50,000 D, and the PEG molecule is attached to the hinge region of the antibody fragment.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is linear and at least about 40,000 D in molecular weight.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is linear and has a molecular weight that is at or about 40,000 D to at or about 300,000 D.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is linear and has a molecular weight that is at or about 40,000 D to at or about 100,000 D.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than PEG molecule, and wherein the PEG molecule is linear and has a molecular weight that is at or about 40,000 D to at or about 70,000 D.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is linear and has a molecular weight that is at or about 40,000 D to at or about 50,000 D.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is linear and at least 40,000D in molecular weight, and the PEG molecule is attached to the hinge region ofthe antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is linear and has a molecular weight that is at or about 40,000 D to at or about 300,000 D, and the PEG molecule is attached to the hinge region of the antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is linear and has a molecular weight that is at or about
  • the PEG molecule is attached to the hinge region of the antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is linear and has a molecular weight that is at or about
  • the PEG molecule is attached to the hinge region of the antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is linear and has a molecular weight that is at or about
  • the PEG molecule is attached to the hinge region of the antibody fragment.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is linear and has a molecular weight that is at least about 30,000 D.
  • the conjugate contains an antibody fragment 'elected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is linear and has a molecular weight that is at or about 30,000 D to at or about 300,000 D.
  • the conjugate contains an antibody fragment selected from the group coi ⁇ _.isting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no moi: than 1 PEG molecule, and wherein the PEG molecule is linear and has a molecular weight that is at or about 30,000 D to at or about 100,000 D.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is linear and has a molecular weight that is at or about 30,000 D to at or about 70,000 D.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is linear and has a molecular weight that is at or about 30,000 D to at or about 50,000 D.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is linear and has a molecular weight that is at or about 30,000 D to at or about 40,000 D.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is linear and at least 30,000D in molecular weight, and the PEG molecule is attached to the hinge region ofthe antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is linear and has a molecular weight that is at or about
  • the PEG molecule is attached to the hinge region of the antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is linear and has a molecular weight that is at or about
  • the PEG molecule is attached to the hinge region of the antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab. Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is linear and has a molecular weight that is at or about
  • the PEG molecule is attached to the hinge region of the antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is linear and has a molecular weight that is at or about
  • the PEG molecule is attached to the hinge region of the antibody fragment.
  • the invention provides a conjugate contaii.-.ig an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is linear and has a molecular weight that is at or about
  • the PEG molecule is attached to the hinge region of the antibody fragment.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is branched and has a molecular weight that is at least about 30,000 D.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is branched and has a molecular weight that is at or about 30,000 D to at or about 300,000 D.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is branched and has a molecular weight that is at or about 30,000 D to at or about 100,000 D.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is branched and has a molecular weight that is at or about 30,000 D to at or about 70,000 D.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is branched and has a molecular weight that is at or about 30,000 D to at or about 50,000 D.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is branched and has a molecular weight that is at or about 30,000 D to at or about 40,000 D.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is branched and at least 30,000D in molecular weight, and the PEG molecule is attached to the hinge region ofthe antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein, the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is branched and has a molecular weight that is at or about 30,000 D to at or about 300,000 D, and the PEG molecule is attached to the hinge region ofthe antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the ..ntibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is branched and has a molecular weight that is at or about 30,000 D to at or about 100,000 D, and the PEG molecule is attached to the hinge region of the antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is branched and has a molecular weight that is at or about 30,000 D to at or about 70,000 D, and the PEG molecule is attached to the hinge region of the antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is branched and has a molecular weight that is at or about 30,000 D to at or about 50,000 D, and the PEG molecule is attached to the hinge region of the antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is branched and has a molecular weight that is at or about 30,000 D to at or about 40,000 D, and the PEG molecule is attached to the hinge region of the antibody fragment.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is linear and has a molecular weight that is at least about 20,000 D.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is linear and has a molecular weight that is at or about 20,000 D to at or about 300,000 D.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is linear and has a molecular weight that is at or about 20,000 D to at or about 100,000 D.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is linear and has a molecular weight that is at or about 20,000 D to at or about 70,000 D.
  • the conjugate ontains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is linear and has a molecular weight that is at or about 20,000 D to at or about 50,000 D.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is linear and has a molecular weight that is at or about 20,000 D to at or about 40,000 D.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is linear and has a molecular weight that is at or about 20,000 D to at or about 30,000 D.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is linear and at least 20,000D in molecular weight, and the PEG molecule is attached to the hinge region ofthe antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is linear and has a molecular weight that is at or about 20,000 D to at or about 300,000 D, and the PEG molecule is attached to the hinge region of the antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is linear and has a molecular weight that is at or about
  • the PEG molecule is attached to the hinge region of the antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is linear and has a molecular weight that is at or about
  • the PEG molecule is attached to the hinge region of the antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is linear and has a molecular weight that is at or about
  • the PEG molecule is attached to the hinge region of the antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is linear and has a molecular weight that is at or about
  • the PEG moiecule is attached to the hinge region of the antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is linear and has a molecular weight that is at or about
  • the PEG molecu:;. is attached to the hinge region of the antibody fragment.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is branched and has a molecular weight that is at least about 20,000 D.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is branched and has a molecular weight that is at or about 20,000 D to at or about 300,000 D.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is branched and has a molecular weight that is at or about 20,000 D to at or about 100,000 D.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab. Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is branched and has a molecular weight that is at or about 20,000 D to at or about 70,000 D.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is branched and has a molecular weight that is at or about 20,000 D to at or about 50,000 D.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is branched and has a molecular weight that is at or about 20,000 D to at or about 40,000 D.
  • the conjugate contains an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, and wherein the PEG molecule is branched and has a molecular weight that is at or about 20,000 D to at or about 30,000 D.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is branched and at least 20,000D in molecular weight, and the PEG molecule is attached to the hinge region ofthe antibody fragment.
  • the in vention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is branched and has a molecular weight that is at or about 20,000 D to at or about 300,000 D, and the PEG molecule is attached to the hinge region of the antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is branched and has a molecular weight that is at or about 20,000 D to at or about 100,000 D, and the PEG molecule is attached to the hinge region of the antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is branched and has a molecular weight that is at or about 20,000 D to at or about 70,000 D, and the PEG molecule is attached to the hinge region of the antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is branched and has a molecular weight that is at or about 20,000 D to at or about 50,000 D, and the PEG molecule is attached to the hinge region of the antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is branched and has a molecular weight that is at or about 20,000 D to at or about 40,000 D, and the PEG molecule is attached to the hinge region of the antibody fragment.
  • the invention provides a conjugate containing an antibody fragment selected from the group consisting of Fab, Fab', and Fab'-SH, wherein the antibody fragment is attached to no more than 1 PEG molecule, wherein the PEG molecule is branched and has a molecular weight that is at or about 20,000 D to at or about 30,000 D, and the PEG molecule is attached to the hinge region of the antibody fragment.
  • the invention provides any of the above-described conjugates wherein the conjugate contains no more than one antibody fragment.
  • conjugates wherein the conjugate contains one or more antibody fragment(s) covalently linked to one or more polymer molecule(s), such as conjugates containing two or more antibody fragments covalently linked together by polymer molecule(s).
  • a polymer molecule is used to link together two antibody fragments to form a dumbbell-shaped structure.
  • conjugates formed by more than two antibody fragments joined by polymer molecule(s) to form a rosette or other shapes can be of the same or different fragment type and can have the same antigen specificity or have different antigen specificities.
  • Such structures can be made by using a polymer molecule derivatized with multiple functional groups permitting the direct attachment, or the attachment by means of bi- or multi-functional linkers, of two or more antibody fragments to the polymer backbone.
  • the invention encompasses any of the above-described conjugates utilizing an antibody fragment comprising an antigen recognition site that binds to rabbit IL-8 and/or human IL-8.
  • the invention encompasses any of the above-described conjugates utilizing an antibody fragment comprising 6G4.2.5LV/L1N35 ⁇ or 6G4.2.5LV/L1N35E as defined below.
  • the invention encompasses any of the above-described conjugates utilizing an antibody fragment comprising 6G4.5.2.5HV1 1 as defined below.
  • the invention encompasses any of the above-described conjugates utilizing an antibody fragment comprising hu6G4.2.5LV/LlN35A or hu6G4.2.5LV/LlN35E as defined below.
  • the invention encompasses any of the above-described conjugates utilizing an antibody fragment comprising hu6G4.2.5HV. Further encompassed herein are any of the above- described conjugates utilizing an antibody fragment comprising 6G4.2.5LV L1N35A or 6G4.2.5LV/L1N35E and further comprising the CDRs of 6G4.2.5HV as defined below.
  • any of the above described conjugates utilizing an antibody fragment comprising hu6G4.2.5LV/LlN35A or hu6G4.2.5LV/LlN35E and further comprising hu6G4.2.5HV as defined below. Additionally encompassed herein are any of the above-described conjugates utilizing an antibody fragment comprising 6G4.2.5LV1 1N35A or 6G4.2.5LV11N35E as defined below. Further provided herein are any of the above- described conjugates utilizing an antibody fragment comprising 6G4.2.5LV1 1N35A or 6G4.2.5LV11N35E and further comprising 6G4.2.5HV11 as defined below, a.
  • Antibody fragments can be produced by any method known in the art.
  • an antibody fragment is derived from a parental intact antibody
  • the parental antibody can be generated by raising polyclonal sera against the desired antigen by multiple subcutaneous (sc) or intraperitoneal (ip) injections of antigen and an adjuvant, such as monophosphoryl hpid A (MPL)/trehalose dicrynomycolate (TDM) (Ribi Immunochem Research, Inc , Hamilton, MT), at multiple sites Two weeks later the animals are boosted 7 to 14 days later animals are bled and the serum is assayed for anti-antigen titer Animals are boosted until titer plateaus Sera are harvested from animals, and polyclonal antibodies are isolated from sera by conventional immunoglobulin purification procedures, such as protein A-Sepharose chromatography, hydroxylapatite chromatography, gel filtration, dialysis, or antigen affinity chromatography
  • the desired antibody fragments can be generated from purified polyclon
  • antibody fragments are derived from monoclonal antibodies generated against the desired antigen
  • Monoclonal antibodies may be made using the hybridoma method first described by Kohler et al Nature 256 495 (1975), or may be made by recombinant DNA methods (U S Patent No 4,816,567)
  • a mouse or other appropriate host animal such as a hamster or macaque monkey
  • lymphocytes may be immunized in vitro Lymphocytes then are fused with myeloma cells using a suitable fusing agent, such as polyethylene glycol, to form a hybridoma cell (Goding, Monoclonal Antibodies Principles and Practice, pp 59-103 (Academic Press, 1986))
  • the hybridoma cells thus prepared are seeded and grown in a suitable culture medium that preferably contains one or more substances that inhibit the growth or survival ofthe unfused, parental myeloma cells
  • a suitable culture medium that preferably contains one or more substances that inhibit the growth or survival ofthe unfused, parental myeloma cells
  • the culture medium for the hyb ⁇ domas typically will include hypoxanthine, aminopte ⁇ n, and thymidine (HAT medium), which substances prevent the growth of HGPRT-deficient cells
  • Preferred myeloma cells are those that fuse efficiently, support stable high-level production of antibody by the selected antibody-producing cells, and are sensitive to a medium such as HAT medium
  • preferred myeloma cell lines are mu ⁇ ne myeloma lines, such as those derived from MOP-21 and M C - 1 1 mouse tumors available from the Salk Institute Cell Distribution Center, San Diego, California USA, and SP-2 or X63-Ag8-653 cells available from the American Type Culture Collection, Rockville, Maryland USA
  • Human myeloma and mouse-human heteromyeloma cell lines also have been described for the production of human monoclonal antibodies (Kozbor, J Immunol , 133 3001 (1984), Brodeur et al , Monoclonal Antibody Production Techniques and Applications, pp 51-63 (Marcel Dekker, Inc , New York, 1987)) Culture medium in which hybridoma cells are growing is assayed for production of monoclonal antibodies directed against the antigen
  • the clones may be subcloned by limiting dilution procedures and grown by standard methods (Goding, Monoclonal Antibodies Principles and Practice, pp 59-103 (Academic Press, 1986)) Suitable culture media for this purpose include, for example, D-MEM or RPMI- 1640 medium
  • the hybridoma cells may be grown in vivo as ascites tumors in an animal
  • the monoclonal antibodies secreted by the subclones are suitably separated from the culture medium, ascites fluid, or serum by conventional immunoglobulin purification procedures such as, for example, protein A-Sepharose, hydroxylapatite chromatography, gel electrophoresis, dialysis, or affinity chromatography
  • DNA encoding the monoclonal antibodies is readily isolated and sequenced using conventional procedures (e g , by using oligonucleotide probes that are capable of binding specifically to genes encoding the heavy and light chains ofthe monoclonal antibodies)
  • the hybridoma cells serve as a preferred source of such DNA
  • the DNA may be placed into expression vectors, which are then transfected into host cells such as E coli cells, simian COS cells, Chinese hamster ovary (CHO) cells, or myeloma cells that do not otherwise produce immunoglobulin protein, to obtain the synthesis of monoclonal antibodies in the recombinant host cells
  • host cells such as E coli cells, simian COS cells, Chinese hamster ovary (CHO) cells, or myeloma cells that do not otherwise produce immunoglobulin protein
  • Review articles on recombinant expression in bacteria of antibody-encoding DNA include Skerra et al , Curr Opinion in Immunol , 5
  • Three-dimensional immunoglobulin models are commonly available and are familiar to those skilled in the art.
  • Computer programs are available which illustrate and display probable three-dimensional conformational structures of selected candidate immunoglobulin sequences. Inspection of these displays permits analysis of the likely role of the residues in the functioning of the candidate immunoglobulin sequence, i.e., the analysis of residues that influence the ability of the candidate immunoglobulin to bind to its antigen.
  • FR residues can be selected and combined from the consensus and import sequences so that the desired antibody characteristic, such as increased affinity for the target antigen(s), is achieved.
  • the CDR residues are directly and most substantially involved in influencing antigen binding.
  • antibody fragments for use herein can be derived from human monoclonal antibodies.
  • Human monoclonal antibodies against the antigen of interest can be made by the hybridoma method.
  • Human myeloma and mouse-human heteromyeloma cell lines for the production of human monoclonal antibodies have been described, for example, by Kozbor J. Immunol, 133: 3001 (1984); Brodeur et al., Monoclonal Antibody Production Techniques and Applications, pp. 51-63 (Marcel Dekker, Inc., New York, 1987); and Boerner et a!., J. Immunol., 147: 86 (1991).
  • transgenic animals e.g. mice
  • transgenic animals e.g. mice
  • JH antibody heavy-chain joining region
  • chimeric and germ-line mutant mice results in complete inhibition of endogenous antibody production.
  • Transfer of the human germ-line immunoglobulin gene array in such germ-line mutant mice will result in the production of human antibodies upon antigen challenge.
  • Jakobovits et al. Proc. Natl. Acad. Sci USA, 90: 2551 (1993); Jakobovits et al., Nature, 362: 255 (1993); Bruggermann et al, Year in Immunol, 7: 33 (1993).
  • phage display technology can be used to produce human antibodies and antibody fragments in vitro, from immunoglobulin variable (V) domain gene repertoires from unimmunized donors.
  • V domain genes are cloned in- frame into either a major or minor coat protein gene of a filamentous bacteriophage, such as M13 or fd, and displayed as functional antibody fragments on the surface of the phage particle. Because the filamentous particle contains a single-stranded DNA copy of the phage genome, selections based on the functional properties ofthe antibody also result in selection ofthe gene encoding the antibody exhibiting those properties.
  • the phage mimics some of the properties of the B-cell.
  • Phage display can be performed in a variety of formats; for their review see, e.g., Johnson et al., Current Opinion in Structural Biology 3:564 (1993).
  • V-gene segments can be used for phage display. Clackson et al., Nature 352:624 (1991) isolated a diverse array of anti-oxazolone antibodies from a small random combinatorial library of V genes derived from the spleens of immunized mice.
  • a repertoire of V genes from unimmunized human donors can be constructed and antibodies to a diverse array of antigens (including self-antigens) can be isolated essentially following the techniques described by Marks et al., J. Mol. Biol. 222:581 (1991), or Griffith et al., EMBO J. 12:725 (1993).
  • antibody genes accumulate mutations at a high rate (somatic hypermutation).
  • B cells displaying high-affinity surface immunoglobulin are preferentially replicated and differentiated during subsequent antigen challenge. This natural process can be mimicked by employing the technique known as "chain shuffling" (Marks et al., Bio/Technol.
  • Gene shuffling can also be used to derive human antibodies from non-human, e.g. rodent, antibodies, where the human antibody has similar affinities and specificities to the starting non-human antibody.
  • this method which is also called “epitope imprinting"
  • either the heavy or light chain variable region of ⁇ _ non-human antibody fragment obtained by phage display techniques as described abo ⁇ e is replaced with a repertoire of human V domain genes, creating a population of non-human chain/human chain scFv or Fab chimeras.
  • bispecific and heteroconjugate antibody fragments having specificities for at least two different antigens can be prepared as full length antibodies or as antibody fragments (e.g. F(ab') 2 bispecific antibody fragments). Antibody fragments having more than two valencies (e.g. trivalent or higher valency antibody fragments) are also contemplated for use herein. Bispecific antibodies, heteroconjugate antibodies, and multi-valent antibodies can be prepared as described in Section (H)(3)(C) below.
  • DNA encoding the monoclonal antibody or antibody fragment of interest can be isolated from its hybridoma or phage display clone of origin, and then manipulated to create humanized and/or affinity matured constructs.
  • known techniques can be employed to introduce an amino acid residue or residues into any desired location on the polypeptide backbone of the antibody fragment, e.g. a cysteine residue placed in the hinge region of the heavy chain, thereby providing a site for specific attachment of polymer molecule(s).
  • the native cysteine residue in either the light or heavy chain of the antibody fragment that would ordinarily form the disulfide bridge linking the light and heavy chains is substituted with another amino acid, such as serine, in order to leave the partner cysteine residue in the opposite chain with a free suflhydryl for specific attachment of polymer molecule.
  • the clone can be used for recombinant production of the antibody fragment as described in Section (H)(4) below.
  • the antibody or antibody fragment product can be recovered from host cell culture and purified as described in Section (H)(4)(F) below.
  • preferred recombinant production systems include bacterial expression and product recovery procedures utilizing the low pH osmotic shock method described in the "Alternative Fab'-SH Purification" section of Example T below. If a full length antibody is produced, the desired antibody fragment can be obtained therefrom by subjecting the intact antibody to enzymatic digestion according to known methods, e.g. as described in Section (H)(4)(G) below.
  • the antibody fragment-polymer conjugates of the invention can be made by derivatizing the desired antibody fragment with an inert polymer. It will be appreciated that any inert polymer which provides the conjugate with the desired apparent size or which has the selected actual MW as taught herein is suitable for use in constructing the antibody fragment-polymer conjugates ofthe invention. Many inert polymers are suitable for use in pharmaceuticals. See, e.g., Davis et al., Biomedical
  • non-proteinaceous polymer is used.
  • the nonproteinaceous polymer ordinarily is a hydrophilic synthetic polymer, i.e., a polymer not otherwise found in nature.
  • hydrophilic polyvinyl polymers fall within the scope of this invention, e.g. polyvinylalcohol and polyvinylpyrrolidone.
  • polyalkylene ethers such as polyethylene glycol (PEG); polyoxyalkylenes such as polyoxyethylene, polyoxypropylene, and block copolymers of polyoxyethylene and polyoxypropylene (Pluronics); polymethacrylates; carbomers; branched or unbranched polysaccharides which comprise the saccharide monomers D-mannose, D- and L-galactose, fucose, fructose, D-xylose, L-arabinose, D-glucuronic acid, sialic acid, D-galacturonic acid, D-mannuronic acid (e.g.
  • polymannuronic acid or alginic acid
  • D-glucosamine D-galactosamine
  • D-glucose and neuraminic acid including homopolysaccharides and heteropolysaccharides such as lactose, amylopectin, starch, hydroxyethyl starch, amylose, dextrane sulfate, dextran, dextrins, glycogen, or the polysaccharide subunit of acid mucopolysaccharides, e.g. hyaluronic acid; polymers of sugar alcohols such as polysorbitol and polymannitol; heparin or heparon.
  • the polymer prior to cross-linking need not be, but preferably is, water soluble, but the final conjugate must be water soluble.
  • the conjugate exhibits a water solubility of at least about 0.01 mg/ml, and more preferably at least about 0.1 mg/ml, and still more preferably at least about 1 mg/ml.
  • the polymer should not be highly immunogenic in the conjugate form, nor should it possess viscosity that is incompatible with intravenous infusion or injection if the conjugate is intended to be administered by such routes.
  • the polymer contains only a single group which is reactive. This helps to avoid cross-linking of protein molecules. However, it is within the scope herein to maximize reaction conditions to reduce cross-linking, or to purify the reaction products through gel filtration or ion exchange chromatography to recover substantially homogenous derivatives. In other embodiments, the polymer contains two or more reactive groups for the purpose of linking multiple antibody fragments to the polymer backbone. Again, gel filtration or ion exchange chromatography can be used to recover the desired derivative in substantially homogeneous form.
  • the molecular weight ofthe polymer can range up to about 500,000 D, and preferably is at least about 20,000 D, or at least about 30,000 D, or at least about 40,000 D.
  • the molecular weight chosen can depend upon the effective size ofthe conjugate to be achieved, the nature (e.g. structure, such as linear or branched) of the polymer, and the degree of derivatization, i.e. the number of polymer molecules per antibody fragment, and the polymer attachment site or sites on the antibody fragment.
  • the polymer can be covalently linked to the antibody fragment through a multifunctional crosslinking agent which reacts with the polymer and one or more amino acid residues of the antibody fragment to be linked.
  • a multifunctional crosslinking agent which reacts with the polymer and one or more amino acid residues of the antibody fragment to be linked.
  • directly crosslink the polymer by reacting a derivatized polymer with the antibody fragment, or vice versa.
  • the covalent crosslinking site on the antibody fragment includes the N-terminal amino group and epsilon amino groups found on lysine residues, as well as other amino, imino, oarboxyl, sulfhydryl, hydroxyl or other hydrophilic groups.
  • the polymer may be covalently bonded directly to the antibody fragment without the use of a multifunctional (ordinarily bifunctional) crosslinking agent.
  • Covalent binding to amino groups is accomplished by known chemistries based upon cyanuric chloride, carbonyl diimidazole, aldehyde reactive groups (PEG alkoxide plus diethyl acetal of bromoacetaldehyde; PEG plus DMSO and acetic anhydride, or PEG chloride plus the phenoxide of 4-hydroxybenzaldehyde, activated succ;uimidyl esters, activated dithiocarbonate PEG, 2,4,5-trichlorophenylcloroformate or P-nitrophenylcloroformate activated PEG.) Carboxyl groups are derivatized by coupling PEG-amine using carbo iimide.
  • Sulfhydryl groups are derivatized by coupling to maleimido-substituted PEG (e.g. alkoxy-PEG amine plus sulfosuccinimidyl 4-(N- maleimidomethyl)cyclohexane-l-carboxylate) as described in WO 97/10847 published March 27, 1997, or PEG-maleimide commercially available from Shearwater Polymers, Inc., Huntsville, AL).
  • PEG e.g. alkoxy-PEG amine plus sulfosuccinimidyl 4-(N- maleimidomethyl)cyclohexane-l-carboxylate
  • PEG-maleimide commercially available from Shearwater Polymers, Inc., Huntsville, AL.
  • free amino groups on the antibody fragment e.g.
  • epsilon amino groups on lysine residues can be thiolated with 2- imino-thiolane (Traut's reagent) and then coupled to maleimide-containing derivatives of PEG as described in Pedley et al., Br. J. Cancer, 70: 1126-1 130 (1994).
  • the polymer will bear a group which is directly reactive with an amino acid side chain, or the N- or C-terminus of the polypeptide linked, or which is reactive with the multifunctional cross-linking agent.
  • polymers bearing such reactive groups are known for the preparation of immobilized proteins.
  • chemistries here one should employ a water soluble polymer otherwise derivatized in the same fashion as insoluble polymers heretofore employed for protein immobilization. Cyanogen bromide activation is a particularly useful procedure to employ in crosslinking polysaccharides.
  • Water soluble in reference to the starting polymer means that the polymer or its reactive intermediate used for conjugation is sufficiently water soluble to participate in a derivatization reaction.
  • the degree of substitution with such a polymer will vary depending upon the number of reactive sites on the antibody fragment, the molecular weight, hydrophilicity and other characteristics of the polymer, and the particular antibody fragment derivatization sites chosen.
  • the conjugate contains from 1 to about 10 polymer molecules, but greater numbers of polymer molecules attached to the antibody fragments of the invention are also contemplated.
  • the desired amount of derivatization is easily achieved by using an experimental matrix in which the time, temperature and other reaction conditions are varied to change the degree of substitution, after which the level of polymer substitution of the conjugates is determined by size exclusion chromatography or other means known in the art.
  • the polymer e.g.
  • PEG is cross-linked to the antibody fragment by a wide variety of methods known per se for the covalent modification of proteins with nonproteinaceous polymers such as PEG. Certain of these methods, however, are not preferred for the purposes herein. Cyanuronic chloride chemistry leads to many side reactions, including protein cross-linking. In addition, it may be particularly likely to lead to inactivation of proteins containing sulfhydryl groups. Carbonyl diimidazole chemistry (Beauchamp et al, Anal Biochem. 131. 25-33 [1983]) requires high pH (>8.5), which can inactivate proteins. Moreover, since the "activated PEG" intermediate can react with water, a very large molar excess of "activated PEG" over protein is required.
  • PEG derivatives include, but are not limited to, amino-PEG, PEG amino acid esters, PEG-hydrazide, PEG-thiol, PEG-succinate, carboxymethylated PEG, PEG-propionic acid, PEG amino acids, PEG succinimidyl succinate, PEG succinimidyl propionate, succinimidyl ester of carboxymethylated PEG, succinimidyl carbonate of PEG, succinimidyl esters of amino acid PEGs, PEG-oxycarbonylimidazole, PEG-nitrophenyl carbonate, PEG tresylate, PEG-glycidyl ether, PEG- aldehyde, PEG vinylsulfone, PEG-maleimide, PEG-orthopyridyl-disulfide, heteroftmctional PEGs, PEG vinyl derivatives, PEG silanes, and PEG
  • reaction conditions for coupling these PEG derivatives will vary depending on the protein, the desired degree of PEGylation, and the PEG derivative utilized. Some factors involved in the choice of PEG derivatives include: the desired point of attachment (such as lysine or cysteine R-groups), hydrolytic stability and reactivity of the derivatives, stability, toxicity and antigenicity of the linkage, suitability for analysis, etc Specific instructions for the use of any particular derivative are available from the manufacturer
  • conjugates of this invention are separated from the unreacted starting materials by gel filtration or ion exchange HPLC Heterologous species of the conjugates are purified from one another in the same fashion
  • the conjugates may also be purified by ion-exchange chromatography
  • the chemistry of many ofthe electrophihcally activated PEG's results in a reduction of ammo group charge ofthe PEGylated product
  • high resolution ion exchange chromatography can be used to separate the free and conjugated proteins, and to resolve species with different levels of PEGylation
  • the resolution of different species e g containing one or two PEG residues
  • species with difference levels of PEGylation are resolved according to the methods described m WO 96/34015 (International Application No PCT/US96/05550 published October 31, 1996)
  • the conjugate is generated by utilizing the derivatization and purification methods described in Section (T) ofthe Examples below
  • the invention provides any of the above-described conjugates formed by its component parts, l e one or more antibody fragment(s) covalently attached to one or more polymer molecule(s), without any extraneous matter in the covalent molecular structure ofthe conjugate c Other Derivatives of Large Effective Size Comugates
  • any of the above-described conjugates can be modified to contain one or more component(s) in addition to the antibody fragment component(s) and polymer component(s) that form the conjugate, wherein the modification does not alter the essential functional property of the conjugate, namely, the substantially improved serum half-life, MRT and/or serum clearance rate as compared to that of the parental antibody fragment from which the conjugate is derived
  • the invention provides any of the above-described conjugates modified to inco ⁇ orate one or more nonproteinaceous functional group(s)
  • the conjugate can be modified to m'.o ⁇ orate nonproteinaceous labels or reporter molecules, such as radtolabel
  • any conjugate ofthe invention is modified by derivatizing the antibody fragment component with any of the above-described non-proteinaceous labels, wherein the label is directly or indirectly (through a coupling agent) attached to the antibody fragment, and wherein such derivatization of the antibody fragment does not contribute or introduce any polymer moiety into the molecular structure of the conjugate.
  • a coupling agent such as dialdehydes, carbodiimides, dimaleimides, bis-imidates, bis-diazotized benzidine, and the like can be used to tag the antibody fragment with the above-described fluorescent or chemiluminescent labels.
  • both direct and indirect labeling can be used to inco ⁇ orate the selected radionuclide into the conjugate.
  • the phrases "indirect labeling” and “indirect labeling approach” both mean that a chelating agent is covalently attached to the antibody fragment moiety or polymer moiety of the conjugate and at least one raidonuclide is inserted into the chelating agent.
  • Preferred chelating agents and radionuclides are set forth in Srivagtava, S.C. and Mease, R.C., "Progress in Research on Ligands, Nuclides and Techniques for Labeling Monoclonal Antibodies," Nucl. Med.
  • a particularly preferred chelating agent is 1- isothiocycmatobenzyl-3-methyldiothelene triaminepent acetic acid ("MX-DTPA").
  • MX-DTPA 1- isothiocycmatobenzyl-3-methyldiothelene triaminepent acetic acid
  • direct labeling and “direct labeling approach” both mean that a radionuclide is covalently attached directly to the antibody fragment moiety (typically via an amino acid residue) or to the polymer moiety of the conjugate.
  • radionuclides for use in direct labeling of conjugate are provided in Srivagtava and Mease, supra.
  • the conjugate is directly labeled
  • the antibody fragment component of the conjugate is directly or indirectly labeled with any of the above-described radiolabels, wherein such labeling of the antibody fragment does not contribute or introduce any polymer moiety into the molecular structure ofthe conjugate. d. Therapeutic Compositions and Administration of Large Effective Size Conjugates
  • the conjugate of the invention is useful for treating the disease indications that are treated with the parent intact antibody.
  • a conjugate derived from an anti-IL-8 antibody or fragment is useful in the treatment of inflammatory disorders as described in Section (H)(5)(B) below.
  • Such conjugates have prophylactic and therapeutic applications in a broad spectrum of IL-8 mediated diseases, such as inflammatory diseases and asthma, in a manner similar to the widespread efficacy of anti-IL-8 antibodies in the treatment of such disease indications that is known in the art, which treatment indications include: (1) ischemic reperfusion injury of the lung (Sekido et al., Nature, 365: 654 (1993)); (2) acute lung injury and ARDS (WO 96/22785 published August 1, 1996; Folkesson et al., J.
  • the conjugates of the invention mimic the in vitro activities of full- length anti-IL-8 monoclonal antibody (e.g. inhibition of IL-8 binding and activation of human neutrophils as shown in Figs.
  • conjugates of the invention derived from anti-IL-8 antibodies and fragments display the same or substantially similar in vitro and in vivo activities as full length anti-IL-8 monoclonal antibody across a range of different parameters, including pharmacokinetic characteristics and therapeutic endpoints in various animal models, the data support the efficacy of the conjugates in the same broad spectrum of disease indications that responds to full length anti- IL-8 antibody treatment.
  • any conjugate ofthe invention derived from an anti-IL-8 antibody or fragment can be advantageously utilized in a method of treating an IL-8 mediated disease or disorder, such as inflammatory diseases.
  • the invention provide 1 , a method of treating an inflammatory disorder in a mammal comprising administering to the mammal an effective amount of a conjugate selected from the group consisting of: (1) every conjugate described in Section (H)(1) above formed by its component parts, i.e.
  • the antibody fragment or fragments and the nonproteinaceous polymer or polymer molecules that form the conjugate without any extraneous matter in the covalent molecular structure of the conjugate, (2) every conjugate described in Section (H)(1) above modified to contain one or more additional components, in addition to the antibody fragment component(s) and polymer component(s) that form the conjugate, wherein the modification does not alter the essential functional property of the conjugate of substantially improved serum half-life, MRT and/or serum clearance rate as compared to that of the parental antibody fragment from which the conjugate is derived, (3) every conjugate described in Section (H)(1) above modified to inco ⁇ orate one or more nonproteinaceous labels or reporter molecules, and (4) every conjugate described in Section (H)(1) above modified to inco ⁇ orate one or more radiolabels; wherein at least one antibody fragment in the conjugate comprises an antigen binding site that binds to human IL-8.
  • the invention encompasses the foregoing method of treating inflammatory disorders wherein at least one antibody fragment in the conjugate is selected from the group consisting of: (1) an antibody fragment comprising 6G4.2.5LV/L1N35A as defined below; (2) an antibody fragment comprising 6G4.2.5LV/L1N35E as defined below; (3) an antibody fragment comprising 6G4.2.5HV1 1 as defined below; (4) an antibody fragment comprising hu6G4.2.5LV/LlN35A as defined below; (5) an antibody fragment comprising hu6G4.2.5LV/LlN35E as defined below; (6) an antibody fragment comprising hu6G4.2.5HV as defined below; (7) an antibody fragment comprising 6G4.2.5LV/L1N35A and further comprising the CDRs of 6G4.2.5HV11 as defined below; (8) an antibody fragment comprising 6G4.2.5LV/L1N35E and further comprising the CDRs of 6G4.2.5HV 1 1 as defined below; (9) an antibody fragment comprising h
  • the invention encompasses any of the foregoing methods of treating an inflammatory disorder wherein the conjugate contains no more than one antibody fragment, wherein the antibody fragment is selected from the group consisting of Fab, Fab' and Fab'-SH, wherein the antibody fragment is covalently attached to no more than one nonproteinaceous polymer molecule, and wherein the nonproteinaceous polymer molecule is a linear polyethylene glycol having a molecular weight of at least at or about 20 kD, or at least at or about 30 kD or at least at or about 40 kD, or is a branched polyethylene glycol having a molecular weight of at least at or about 40 kD.
  • the invention provides a method of treating ischemic reperfusion injury in a mammal comprising administering to the mammal an effective amount of a conjugate selected from the group consisting of: (1) every conjugate described in Section (H)(1) above formed by its component parts, i.e.
  • the antibody fragment or fragments and the nonproteinaceous polymer or polymer molecules that form the conjugate without any extraneous matter in the covalent molecular structure of the conjugate, (2) every conjugate described in Section (H)(1) above modified to contain one or more additional components, in addition to the antibody fragment component(s) and polymer component(s) that form the conjugate, wherein the modification does not alter the essential functional property of the conjugate of substantially improved serum half-life, MRT and/or serum clearance rate as compared to that of the parental antibody fragment from which the conjugate is derived, (3) every conjugate ot-ccribed in Section (H)(1) above modified to inco ⁇ orate one or more nonproteinaceous labels or reporter molecules, and (4) every conjugate described in Section (H)(1) above modified to inco ⁇ orate one or more radiolabels; wherein at least one antibody fragment in the conjugate comprises an antigen binding site that binds to human IL-8.
  • the invention encompasses the foregoing method of treating ischemic reperfusion injury wherein at least one antibody fragment in the conjugate is selected from the group consisting of: (1) an antibody fragment comprising 6G4.2.5LV/L1N35A as defined below; (2) an antibody fragment comprising 6G4.2.5LV/L1N35E as defined below; (3) an antibody fragment comprising 6G4.2.5HV1 1 as defined below; (4) an antibody fragment comprising hu6G4.2.5LV LlN35A as defined below; (5) an antibody fragment comprising hu6G4.2.5LV LlN35E as defined below; (6) an antibody fragment comprising hu6G4.2.5HV as defined below; (7) an antibody fragment comprising 6G4.2.5LV/L1N35A and further comprising the CDRs of 6G4.2.5HV1 1 as defined below; (8) an antibody fragment comprising 6G4.2.5LV/L1N35E and further comprising the CDRs of 6G4.2.5HV11 as defined below; (9) an antibody fragment comprising
  • the invention encompasses the foregoing methods of treating ischemic reperfusion injury wherein the ischemic reperfusion injury is induced by or incident to a surgical procedure, i.e. a surgical tissue reperfusion injury.
  • the invention encompasses the foregoing methods of treating ischemic reperfusion injury wherein the ischemic reperfusion injury is a myocardial ischemic reperfusion injury, such as myocardial infarction, reperfusion after cardiac surgery, cardiac arrest, and constriction after percutaneous transluminal coronary angioplasty.
  • myocardial ischemic reperfusion injury such as myocardial infarction, reperfusion after cardiac surgery, cardiac arrest, and constriction after percutaneous transluminal coronary angioplasty.
  • the invention encompasses any of the foregoing methods of treating ischemic reperfusion injury wherein the conjugate contains no more than one antibody fragment, wherein the antibody fragment is selected from the group consisting of Fab, Fab' and Fab'-SH, wherein the antibody fragment is covalently attached to no more than one nonproteinaceous polymer molecule, and wherein the nonproteinaceous polymer molecule is a linear polyethylene glycol having a molecular weight of at least at or about 20 kD, or at least at or about 30 kD or at least at or about 40 kD, or is a branched polyethylene glycol having a molecular weight of at least at or about 40 kD.
  • the invention provides a method of treating acute lung injury in a mammal comprising administering to the mammal ar- effective amount of a conjugate selected from the group consisting of: (1) every conjugate described in Section (H)(1) above formed by its component parts, i.e.
  • the antibody fragment or fragments and the nonproteinaceous polymer or polymer molecules that form the conjugate without any extraneous matter in the covalent molecular structure of the conjugate, (2) every conjugate described in Section (H)(1) above modified to contain one or more additional components, in addition to the antibody fragment component(s) and polymer component(s) that form the conjugate, wherein the modification does not alter the essential functional property of the conjugate of substantially improved serum half-life, MRT and or serum clearance rate as compared to that of the parental antibody fragment from which the conjugate is derived, (3) every conjugate described in Section (H)(1) above modified to inco ⁇ orate one or more nonproteinaceous labels or reporter molecules, and (4) every conjugate described in Section (H)(1) above modified to inco ⁇ orate one or more radiolabels; wherein at least one antibody fragment in the conjugate comprises an antigen binding site that binds to human IL-8.
  • the invention encompasses the foregoing method of treating acute lung injury wherein at least one antibody fragment in the conjugate is selected from the group consisting of: (1) an antibody fragment comprising 6G4.2.5LV/L1N35A as defined below; (2) an antibody fragment comprising 6G4.2.5LV/L1N35E as defined below; (3) an antibody fragment comprising 6G4.2.5HV1 1 as defined below; (4) an antibody fragment comprising hu6G4.2.5LV/LlN35A as defined below; (5) an antibody fragment comprising hu6G4.2.5LV/LlN35E as defined below; (6) an antibody fragment comprising hu6G4.2.5HV as defined below; (7) an antibody fragment comprising 6G4.2.5LV/L1N35A and further comprising the CDRs of 6G4.2.5HV1 1 as defined below; (8) an antibody fragment comprising 6G4.2.5LV/L1N35E and further comprising the CDRs of 6G4.2.5HV1 1 as defined below; (9) an antibody fragment comprising
  • the invention encompasses the foregoing methods of treating acute lung injury wherein the acute lung injury includes adult respiratory distress syndrome (ARDS).
  • ARDS adult respiratory distress syndrome
  • the invention encompasses any of the foregoing methods of treating acute lung injury wherein the conjugate contains no more than one antibody fragment, wherein the antibody fragment is selected from the group consisting of Fab, Fab' and Fab'-SH, wherein the antibody fragment is covalently attached to no more than one nonproteinaceous polymer molecule, and wherein the nonproteinaceous polymer molecule is a linear polyethylene glycol having a molecular weight of at least at or about 20 kD, or at least at or about 30 kD or at least at or about 40 kD.
  • the invention encompasses any of the foregoing methods of treating acute lung injury, wherein the patient is selected for prophylactic treatment prior to onset of acute lung injury (with or without progression to ARDS), such as at least 2 hours prior to onset, or at least 90 minutes prior to onset, or at least 60 minutes prior to onset, or at least 30 minutes prior to onset, by the assessment of biological parameters displayed in the patient's condition that indicate likely progression of disease to acute lung injury which may include ARDS, e.g.
  • the conjugate contains no more than one antibody fragment
  • the antibody fragment is selected from the group consisting of Fab, Fab' and Fab'-SH, wherein the antibody fragment is covalently attached to no more than one nonproteinaceous polymer molecule
  • the nonproteinaceous polymer molecule is a linear polyethylene glycol having a molecular w eight of at least at or about 20 kD, or at least at or about 30 kD or at least at or about 40 kD, or is a branched polyethylene glycol having a molecular weight of at least at or about 40 kD.
  • the invention provides a method of treating hypovolemic shock in a mammal comprising administering to the mammal an effective amount of a conjugate selected from the group consisting of: (1) every conjugate described in Section (H)(1) above formed by its component parts, i.e.
  • the antibody fragment or fragments and the nonproteinaceous polymer or polymer molecules that form the conjugate without any extraneous matter in the covalent molecular structure of the conjugate, (2) every conjugate described in Section (H)(1) above modified to contain one or more additional components, in addition to the antibody fragment component(s) and polymer component(s) that form the conjugate, wherein the modification does not alter the essential functional property ofthe conjugate of substantially improved serum half-life, MRT and/or serum clearance rate as compared to that of the parental antibody fragment from which the conjugate is derived, (3) every conjugate described in Section (H)(1) above modified to inco ⁇ orate one or more nonproteinaceous labels or reporter molecules, and (4) every conjugate described in Section (H)(1) above modified to inco ⁇ orate one or more radiolabels; wherein at least one antibody fragment in the conjugate comprises an antigen binding site that binds to human IL-8.
  • the invention encompasses the foregoing method of treating hypovolemic shock wherein at least one antibody fragment in the conjugate is selected from the group consisting of: (1) an antibody fragment comprising 6G4.2.5LV/L1N35A as defined below; (2) an antibody fragment comprising 6G4.2.5LV/L1N35E as defined below; (3) an antibody fragment comprising 6G4.2.5HV1 1 as defined below; (4) an antibody fragment comprising hu6G4.2.5LV/LlN35A as defined below; (5) an antibody fragment comprising hu6G4.2.5LV/LlN35E as defined below; (6) an antibody fragment comprising hu6G4.2.5HV as defined below; (7) an antibody fragment comprising 6G4.2.5LV/L1N35A and further comprising the CDRs of 6G4.2.5HV11 as defined below; (8) an antibody fragment comprising 6G4.2.5LV/L1N35E and further comprising the CDRs of 6G4.2.5HV1 1 as defined below; (9) an antibody fragment comprising
  • the invention encompasses any of the foregoing methods of treating hypovolemic shock wherein the conjugate contains no more than one antibody fragment, wherein the antibody fragment is selected from the group consisting of Fab, Fab' and Fab'-SH, wherein the antibody fragment is covalently attached to no :nore than one nonproteinaceous polymer molecule, and wherein the nonproteinaceous polymer molecule is a linear polyethylene glycol having a molecular weight of at least at or about 20 kD, or at least at or about 30 kD or at least at or about 40 kD, or is a branched polyethylene glycol having a molecular weight of at least at or about 40 kD.
  • the invention provides a method of treating an inflammatory bowel disease in a mammal comprising administering to the mammal an effective amount of a conjugate selected from the group consisting of: (1 ) every conjugate described in Section (H)(1) above formed by its component parts, i.e.
  • the antibody fragment or fragments and the nonproteinaceous polymer or polymer molecules that form the conjugate without any extraneous matter in the covalent molecular structure of the conjugate, (2) every conjugate described in Section (H)(1) above modified to contain one or more additional components, in addition to the antibody fragment component(s) and polymer component(s) that form the conjugate, wherein the modification does not alter the essential functional property of the conjugate of substantially improved serum half-life, MRT and/or serum clearance rate as compared to that of the parental antibody fragment from which the conjugate is derived, (3) every conjugate described in Section (H)(1) above modified to inco ⁇ orate one or more nonproteinaceous labels or reporter molecules, and (4) every conjugate described in Section (H)(1) above modified to inco ⁇ orate one or more radiolabels; wherein at least one antibody fragment in the conjugate comprises an antigen binding site that binds to human IL-8.
  • the invention encompasses the foregoing method of treating an inflammatory bowel disease wherein at least one antibody fragment in the conjugate is selected from the group consisting of: (1) an antibody fragment comprising 6G4.2.5LV/L1N35A as defined below; (2) an antibody fragment comprising 6G4.2.5LV/L1N35E as defined below; (3) an antibody fragment comprising 6G4.2.5HV1 1 as defined below; (4) an antibody fragment comprising hu6G4.2.5LV/LlN35A as defined below; (5) an antibody fragment comprising hu6G4.2.5LV/LlN35E as defined below; (6) an antibody fragment comprising hu6G4.2.5HV as defined below; (7) an antibody fragment comprising 6G4.2.5LV/L1N35A and further comprising the CDRs of 6G4.2.5HV1 1 as defined below; (8) an antibody fragment comprising 6G4.2.5LV/L1N35E and further comprising the CDRs of 6G4.2.5HV1 1 as defined below; (9) an antibody fragment fragment comprising
  • the invention encompasses the foregoing methods of treating an inflammatory bowel disease wherein the inflammatory bowel disease is ulcerative colitis.
  • the invention encompasses any of the foregoing methods of treating inflammatory bowel disease wherein the conjugate contains no more than one antibody fragment, wherein the antibody fragment is selected from the group consisting of Fab, Fab' and Fab'-SH, wherein the antibody fragment is covalently attached to no more than one nonproteinaceous polymer molecule, and wherein the nonproteinaceous polymer molecule is a linear polyethylene glycol having a molecular weight of at least at or about 20 kD, or at least administrat. or about 30 kD or at least at or about 40 kD, or is a branched polyethylene glycol having a molecular weight of at least at or about 40 kD.
  • the invention provides a method of treating a bacterial pneumonia in a mammal comprising administering to the mammal an effective amount of a conjugate selected from the group consisting of: (1) every conjugate described in Section (H)(1) above formed by its component parts, i.e.
  • the antibody fragment or fragments and the nonproteinaceous polymer or polymer molecules that form the conjugate without any extraneous matter in the covalent molecular structure of the conjugate, (2) every conjugate described in Section (H)(1) above modified to contain one or more additional components, i._ addition to the antibody fragment component(s) and polymer component(s) that form the conjugate, wherein the modification does not alter the essential functional property of the conjugate of substantially improved serum half-life, MRT and/or serum clearance rate as compared to that of the parental antibody fragment from which the conjugate is derived, (3) every conjugate described in Section (H)(1) above modified to inco ⁇ orate one or more nonproteinaceous labels or reporter molecules, and (4) every conjugate described in Section (H)(1) above modified to inco ⁇ orate one or more radiolabels; wherein at least one antibody fragment in the conjugate comprises an antigen binding site that binds to human IL-8.
  • the invention encompasses the foregoing method of treating bacterial pneumonia wherein at least one antibody fragment in the conjugate is selected from the group consisting of: (1) an antibody fragment comprising 6G4.2.5LV/L1N35A as defined below; (2) an antibody fragment comprising 6G4.2.5LV/L1N35E as defined below; (3) an antibody fragment comprising 6G4.2.5HV1 1 as defined below; (4) an antibody fragment comprising hu6G4.2.5LV/LlN35A as defined below; (5) an antibody fragment comprising hu6G4.2.5LV/LlN35E as defined below; (6) an antibody fragment comprising hu6G4.2.5HV as defined below; (7) an antibody fragment comprising 6G4.2.5LV/L1N35A and further comprising the CDRs of 6G4.2.5HV11 as defined below; (8) an antibody fragment comprising 6G4.2.5LV/L1N35E and further comprising the CDRs of 6G4.2.5HV1 1 as defined below; (9) an antibody fragment comprising h
  • the invention encompasses any of the foregoing methods of treating bacterial pneumonia wherein the conjugate contains no more than one antibody fragment, wherein the antibody fragment is selected from the group consisting of Fab, Fab' and Fab'-SH, wherein the antibody fragment is covalently attached to no more than one nonproteinaceous polymer molecule, and wherein the nonproteinaceous polymer molecule is a linear polyethylene glycol having a molecular weight of at least at or about 20 kD, or at least at or about 30 kD or at least at or about 40 kD, or is a branched polyethylene glycol having a molecular weight of at least at or about 40 kD.
  • the invention provides a method of treating an asthmatic disease in a mammal comprising administering to the mammal an effective amount of a conjugate selected from the group consisting of: (1) every conjugate described in Section (H)(1) above formed by its component parts, i.e.
  • the antibody fragment or fragments and the nonproteinaceous polymer or polymer molecules that form the conjugate without any extraneous matter in the covalent molecular structure of the conjugate, (2) every conjugate described in Section (H)(1) above modified to contain one or more additional components, in addition to the antibody fragment component(s) and polymer component(s) that form the conjugate, wherein the modification does not alter the esse ⁇ _..al functional property ofthe conjugate of substantially improved serum half-life, MR'i and/or serum clearance rate as compared to that of the parental antibody fragment from which the conjugate is derived, (3) e ery conjugate described in Section (H)(1) above modified to inco ⁇ orate one c.' more nonproteinaceous labels or reporter molecules, and (4) every conjugate described in Section (H)(1) above modified to inco ⁇ orate one or more radiolabels; wherein at least one antibody fragment in the conjugate comprises an antigen binding site that binds to human IL-8.
  • the invention encompasses the foregoing method of treating an asthmatic disease wherein at least one antibody fragment in the conjugate is selected from the group consisting of: (1) an antibody fragment comprising 6G4.2.5LV/L1N35A as defined below; (2) an antibody fragment comprising 6G4.2.5LV L1N35E as defined below; (3) an antibody fragment comprising 6G4.2.5HV1 1 as defined below; (4) an antibody fragment comprising hu6G4.2.5LV/LlN35A as defined below; (5) an antibody fragment comprising hu6G4.2.5LV/LlN35E as defined below; (6) an antibody fragment comprising hu6G4.2.5HV as defined below; (7) an antibody fragment comprising 6G4.2.5LV/L1N35A and further comprising the CDRs of 6G4.2.5HV11 as defined below; (8) an antibody fragment comprising 6G4.2.5LV/L1N35E and further comprising the CDRs of 6G4.2.5HV11 as defined below; (9) an antibody fragment comprising hu
  • the invention encompasses the foregoing methods of treating asthmatic disease wherein the asthmatic disease is allergic asthma.
  • the invention encompasses any of the foregoing methods of treating an asthmatic disease wherein the conjugate contains no more than one antibody fragment, wherein the antibody fragment is selected from the group consisting of Fab, Fab' and Fab'-SH, wherein the antibody fragment is covalently attached to no more than one nonproteinaceous polymer molecule, and wherein the nonproteinaceous polymer molecule is a linear polyethylene glycol having a molecular weight of at least at or about 20 kD, or at least at or about 30 kD or at least at or about 40 kD, or is a branched polyethylene glycol having a molecular weight of at least at or about 40 kD.
  • the invention encompasses any of the foregoing methods of treating inflammatory diseases or asthmatic diseases wherein the mammal is a human.
  • Therapeutic formulations of the conjugate of the invention can be prepared by utilizing the same procedures described for the formulation of the anti-IL-8 antibodies and fragments of the invention in Section (H)(5)(B) be'ow.
  • the conjugate of the invention can be administered in place of the parent antibody for a given disease indication by modifying the formulation, dosage, administration protocol, and other aspects of a therapeutic regimen as required by the different pharmacodynamic characteristics of the conjugate and as dictated by common medical knowledge and practice. e. Reagent Uses for Large Effective Size Conjugates
  • the conjugate of the invention also finds application as a reagent in an animal model system for in vivo study of the biological functions ofthe antigen recognized by the conjugate.
  • the conjugate would enable the practitioner to inactivate or detect the cognate antigen in circulation or in tissue for a far greater period of time than would be possible with art-known constructs while removing any Fc interaction (which could attend the use of an intact antibody) from the system.
  • the increased half-life of the conjugate of the invention can be applied advantageously to the induction of tolerance for the underivatized antibody fragment in a test animal by employing the Wie et al, Int. Archs. Allergy Appl. Immunol., 64: 84-99 (1981) method for allergen tolerization, which would permit the practitioner to repeatedly challenge the tolerized animal with the underivatized parental antibody fragment without generating an immune response against the parental fragment.
  • the invention provides an antibody fragment or full length antibody comprising a heavy chain comprising the amino acid sequence of amino acids 1-230 (herein referred to as "6G4.2.5HV11 ”) ofthe humanized anti-IL-8 6G4.2.5vl 1 heavy chain polypeptide amino acid sequence of Figs. 37A-37B (SEQ ID NO: 60).
  • the invention encompasses a single chain antibody fragment comprising the 6G4.2.5HV11, with or without any additional amino acid sequence.
  • the invention provides a single chain antibody fragment comprising the 6G4.2.5HV1 1 without any associated light chain amino acid sequence, i.e. a single chain species that makes up one half of a Fab fragment.
  • an antibody or antibody fragment comprising the 6G4.2.5HV11, and further comprising a light chain comprising the amino acid sequence of amino acids 1-219 (herein referred to as "6G4.2.5LV1 1 ") of the humanized anti-IL-8 6G4.2.5vl l light chain polypeptide amino acid sequence of Fig. 3 IB (SEQ ID NO: 51).
  • the invention provides a single chain antibody fragment wherein the 6G4.2.5HV1 1 and the 6G4.2.5LV1 1 are contained in a single chain polypeptide species.
  • the single chain antibody fragment comprises the 6G4.2.5HV11 joined to the 6G4.2.5LV11 by means of a flexible peptide linker sequence, wherein the heavy chain and light chain domains can associate in a "dimeric" structure analogous to that formed in a two-chain Fab species.
  • the single chain antibody fragment is a species comprising the 6G4.2.5HV1 1 joined to the 6G4.2.5LV1 1 by a linker that is too short to permit intramolecular pairing of complementary domains, i.e. a single chain polypeptide monomer that forms a diabody upon dimerization with another monomer.
  • the invention provides an antibody fragment comprising a plurality of polypeptide chains, wherein one polypeptide chain comprises the 6G4.2.5HV1 1 and a second polypeptide chain comprises the 6G4.2.5LV1 1 and the two polypeptide chains are covalently linked by one or more interchain disulfide bonds.
  • the foregoing two-chain antibody fragment is selected from the group consisting of Fab, Fab', Fab'-SH, and F(ab') 2 .
  • the invention also provides an antibody or antibody fragment comprising a heavy chain containing the 6G4.2.5HV1 1 and optionally further comprising a light chain containing the 6G4.2.5LV1 1 , wherein the heavy chain, and optionally the light chain, is (are) fused to an additional moiety, such as additional immunoglobulin constant domain sequence.
  • Constant domain sequence can be added to the heavy chain and/or light chain sequence(s) to form species with full or partial length heavy and/or light chain(s). It will be appreciated that constant regions of any isotype can be used for this pu ⁇ ose, including IgG, IgM, IgA, IgD, and IgE constant regions, and that such constant regions can be obtained from any human or animal species.
  • the constant domain sequence is human in origin. Suitable human constant domain sequences can be obtained from Kabat et al. (supra).
  • the antibody or antibody fragment comprises the 6G4.2.5HV11 in a heavy chain that is fused to or contains a leucine zipper sequence.
  • the leucine zipper can increase the affinity and/or production efficiency of the antibody or antibody fragment of interest.
  • Suitable leucine zipper sequences include the jun and fos leucine zippers taught by Kostelney et al. J. Immunol., 148: 1547-1553 (1992) and the GCN4 leucine zipper described in the Examples below.
  • the antibody or antibody fragment comprises the 6G4.2.5HV1 1 fused at its C-terminus to the GCN4 leucine zipper to yield the amino acid sequence of amino acids 1-275 (herein referred to as "6G4.2.5HV1 1GCN4") of the heavy chain polypeptide amino acid sequence of Figs. 37A-37B (SEQ ID NO: 60).
  • 6G4.2.5HV1 1GCN4 the heavy chain polypeptide amino acid sequence of Figs. 37A-37B (SEQ ID NO: 60).
  • the invention additionally encompasses humanized anti-IL-8 monoclonal antibody and antibody fragments comprising variants of the 6G4 2 5 complementarity determining regions (CDRs) or variants of the 6G4 2 5vl 1 variable domains which exhibit higher affinity for human IL-8 and/or possess properties that yield greater efficiency m recombinant production processes
  • CDRs complementarity determining regions
  • 6G4 2 5vl 1 variable domains which exhibit higher affinity for human IL-8 and/or possess properties that yield greater efficiency m recombinant production processes
  • the invention provides humanized anti-IL-8 monoclonal antibodies and antibody fragments comprising the complementarity determining regions (referred to herein as the "CDRs of 6G4 2 5LV") LI, L2, and L3 of the 6G4 2 5 light chain variable domain ammo acid sequence of Fig 24, wherein LI corresponds to ammo acids 24-39 ofthe amino acid sequence of Fig 24, L2 corresponds to amino acids 55-61 of the amino acid sequence of Fig 24 (SEQ ID NO 35) , and L3 corresponds to amino acids 94- 102 ofthe amino acid sequence of Fig 24 (SEQ ID NO 35)
  • LI corresponds to ammo acids 24-39 ofthe amino acid sequence of Fig 24
  • L2 corresponds to amino acids 55-61 of the amino acid sequence of Fig 24 (SEQ ID NO 35)
  • L3 corresponds to amino acids 94- 102 ofthe amino acid sequence of Fig 24 (SEQ ID NO 35)
  • the invention provides a variant 6G4 2 5 humanized antibody or antibody fragment comprising a humanized light chain variable domain comprising a variant (hereinafter referred to a
  • 6G4 2 5LV CDRs variant of the complementarity determining regions LI, L2, and L3 of the 6G4 2 5 variable light chain domain ammo acid sequence of Fig 24 (SEQ ID NO 35)
  • the invention provides a variant 6G4 2 5 humanized antibody or antibody fragment comprising a 6G4 2 5LV DRs variant (herein referred to as “6G4 2 5LV/L1N35X 35 ") wherein LI corresponds ⁇ ammo acids 24-39 of the amino acid sequence of Fig 24 (SEQ ID NO 35) with the proviso that any amino acid other than Asn
  • the invention provide r variant 6G4 2 5 humanized antibody or antibody fragment comprising a 6G4 2 5LV CDRs variant (herein referred to as "6G4 2 5LV/L1N35A") wherein LI corresponds to amino acids 24-39 of the ammo acid sequence of Fig 24 (SEQ ID NO 35) with the proviso that Ala is substituted for Asn at amino acid position 35, L2 corresponds to amino acids 55-61 ofthe ammo acid sequence of Fig 24 (SEQ ID NO 35), and L3 corresponds to ammo acids 94-102 of the amino acid sequence of Fig 24 (SEQ ID NO 35) In another preferred
  • the invention provides a variant 6G42 5 humanized antibody or antibody fragment comprising a 6G4 2 5LV CDRs variant (herein referred to as "6G4 2 5LV/L1 S26X 26 ") wherein LI corresponds to ammo acids 24-39 ofthe amino acid sequence of Fig 24 (SEQ ID NO 35) with the proviso that any ammo acid other than Ser (denoted as "X 2 ") is substituted for Ser at amino acid position 26, L2 corresponds to amino acids 55-61 ofthe amino acid sequence of Fig. 24 (SEQ ID NO: 35), and L3 corresponds to amino acids 94-102 of the amino acid sequence of Fig. 24 (SEQ ID NO: 35).
  • LI corresponds to ammo acids 24-39 ofthe amino acid sequence of Fig 24 (SEQ ID NO 35) with the proviso that any ammo acid other than Ser (denoted as "X 2 ") is substituted for Ser at amino acid position 26
  • L2 corresponds to amino acids 55-61 ofthe amino acid sequence of
  • the invention provides a variant 6G4.2.5 humanized antibody or antibody fragment comprising a 6G4.2.5LV CDRs variant (herein referred to as "6G4.2.5LV/L1S26A") wherein LI corresponds to amino acids 24-39 of the amino acid sequence of Fig. 24 (SEQ ID NO: 35) with the proviso that Ala is substituted for Ser at amino acid position 26, L2 corresponds to amino acids 55-61 ofthe amino acid sequence of Fig. 24 (SEQ ID NO: 35), and L3 corresponds to amino acids 94-102 ofthe amino acid sequence of Fig. 24 (SEQ ID NO: 35).
  • LI corresponds to amino acids 24-39 of the amino acid sequence of Fig. 24 (SEQ ID NO: 35) with the proviso that Ala is substituted for Ser at amino acid position 26
  • L2 corresponds to amino acids 55-61 ofthe amino acid sequence of Fig. 24 (SEQ ID NO: 35)
  • L3 corresponds to amino acids 94-102 ofthe amino acid sequence of Fig. 24 (S
  • the invention provides a variant 6G4.2.5 humanized antibody or antibody fragment comprising a 6G4.2.5LV CDRs variant (herein referred to as "6G4.2.5LV/L3H98X 98 ") wherein LI corresponds to amino acids 24-39 of the amino acid sequence of Fig. 24 (SEQ ID NO: 35), L2 corresponds to amino acids 55-61 ofthe amino acid sequence of Fig. 24 (SEQ ID NO: 35), and L3 corresponds to amino acids 94-102 ofthe amino acid sequence of Fig. 24 (SEQ ID NO: 35) with the proviso that any amino acid other than
  • the invention provides a variant 6G4.2.5 humanized antibody or antibody fragment comprising a 6G4.2.5LV CDRs variant (herein referred to as "6G4.2.5LV/L3H98A”) wherein LI corresponds to amino acids 24-39 of the amino acid sequence of Fig. 24 (SEQ ID NO: 35), L2 corresponds to amino acids 55-61 of the amino acid sequence of Fig. 24 (SEQ ID NO: 35), and L3 corresponds to amino acids 94-102 of the amino acid sequence of Fig. 24 (SEQ ID NO: 35) with the proviso that Ala is substituted for His at amino acid position 98.
  • LI corresponds to amino acids 24-39 of the amino acid sequence of Fig. 24 (SEQ ID NO: 35)
  • L2 corresponds to amino acids 55-61 of the amino acid sequence of Fig. 24 (SEQ ID NO: 35)
  • L3 corresponds to amino acids 94-102 of the amino acid sequence of Fig. 24 (SEQ ID NO: 35) with the proviso that Ala is substitute
  • the invention provides a variant 6G4.2.5 humanized antibody or antibody fragment comprising a 6G4.2.5LV CDRs variant (herein referred to as "6G4.2.5LV/L1S26X 26 ,N35X 35 ") wherein LI corresponds to amino acids 24-39 ofthe amino acid sequence of Fig. 24 (SEQ ID NO: 35) with the proviso that any amino acid other than Ser (denoted as "X 2 ”) ' s substituted for Ser at amino acid position 26 and any amino acid other than Asn (denoted as "X 3 5”) is substituted for Asn at amino acid position 35, L2 corresponds to amino acids 55-61 of the amino acid sequence of Fig.
  • the invention provides a variant 6G4.2.5 humanized antibody or antibody fragment comprising a 6G4.2.5LV CDRs variant (herein referred to as "6G4.2.5LV/L1S26A.N35A") wherein LI corresponds to amino acids 24- 39 of the amino acid sequence of Fig. 24 (SEQ ID NO:35) with the proviso that Ala is substituted for Ser at amino acid position 26 and Ala is substituted for Asn at amino acid position 35, L2 corresponds to amino acids 55-61 of the amino acid sequence of Fig. 24 (SEQ ID NO 35), and L3 corresponds to amino acids 94-102 of the amino acid sequence of Fig. 24 (SEQ ID NO 35).
  • LI corresponds to amino acids 24- 39 of the amino acid sequence of Fig. 24 (SEQ ID NO:35) with the proviso that Ala is substituted for Ser at amino acid position 26 and Ala is substituted for Asn at amino acid position 35
  • L2 corresponds to amino acids 55-61 of the amino acid sequence of Fig. 24 (S
  • the invention provides a variant 6G4.2.5 humanized antibody or antibody fragment comprising a 6G4.2.5LV CDRs variant (herein referred to as "6G4.2.5LV/L1N35X 35 /L3H98X 98 ") wherein LI corresponds to amino acids 24-39 ofthe amino acid sequence of Fig. 24 (SEQ ID NO 35) with the proviso that any amino acid other than Asn (denoted as "X 3 5") is substituted for Asn at amino acid position 35, L2 corresponds to amino acids 55-61 ofthe amino acid sequence of Fig. 24 (SEQ ID NO 35), and L3 corresponds to amino acids 94-102 ofthe amino acid sequence of Fig.
  • LI corresponds to amino acids 24-39 ofthe amino acid sequence of Fig. 24 (SEQ ID NO 35) with the proviso that any amino acid other than Asn (denoted as "X 3 5") is substituted for Asn at amino acid position 35
  • L2 corresponds to amino acids 55-61 ofthe amino acid sequence of Fig. 24 (
  • the invention provides a variant 6G4.2.5 humanized antibody or antibody fragment comprising a 6G4.2.5LV CDRs variant (herein referred to as "6G4.2.5LV/L1N35A/L3H98A") wherein LI corresponds to amino acids 24-39 of the amino acid sequence of Fig. 24 (SEQ ID NO 35) with the proviso that Ala is substituted for Asn at amino acid position 35, L2 corresponds to amino acids 55-61 of the amino acid sequence of Fig. 24 (SEQ ID NO 35), and L3 corresponds to amino acids 94-102 of the amino acid sequence of Fig. 24 (SEQ ID NO 35) with the proviso that Ala is substituted for His at amino acid position 98.
  • LI corresponds to amino acids 24-39 of the amino acid sequence of Fig. 24 (SEQ ID NO 35) with the proviso that Ala is substituted for Asn at amino acid position 35
  • L2 corresponds to amino acids 55-61 of the amino acid sequence of Fig. 24 (SEQ ID NO 35)
  • the invention provides a variant 6G4.2.5 humanized antibody or antibody fragment comprising a 6G4.2.5LV CDRs variant (herein referred to as "6G4.2.5LV/L1 S26X 26 /L3H98X 98 ") wherein LI corresponds to amino acids 24-39 of the amino acid sequence of Fig. 24 (SEQ ID NO 35) with the proviso that any amino acid other than Ser (denoted as "X 26 ") is substituted for Ser at amino acid position 26, L2 corresponds to amino acids 55-61 of the amino acid sequence of Fig. 24 (SEQ ID NO 35), and L3 corresponds to amino acids 94-102 ofthe amino acid sequence of Fig.
  • LI corresponds to amino acids 24-39 of the amino acid sequence of Fig. 24 (SEQ ID NO 35) with the proviso that any amino acid other than Ser (denoted as "X 26 ") is substituted for Ser at amino acid position 26
  • L2 corresponds to amino acids 55-61 of the amino acid sequence of Fig. 24 (SEQ ID NO 35
  • the invention provides a variant 6G4.2.5 humanized antibody or antibody fragment comprising a 6G4.2.5LV CDRs variant (herein referred to as "6G4.2.5LV/L1S26A/L3H98A”) wherein LI corresponds to amino acids 24-39 of the amino acid sequence of Fig. 24 (SEQ ID NO 35) with the proviso that Ala is substituted for Ser at amino acid position 26, L2 corresponds to amino acids 55-61 of the amino acid sequence of Fig. 24 SEQ ID NO 35), and L3 corresponds to amino acids 94-102 of the amino acid sequence of Fig. 24 (SEQ ID NO: 35) with the proviso that Ala is substituted for His at amino acid position 98.
  • 6G4.2.5 humanized antibody or antibody fragment comprising a 6G4.2.5LV CDRs variant (herein referred to as "6G4.2.5LV/L1S26A/L3H98A”) wherein LI corresponds to amino acids 24-39 of the amino acid sequence of Fig. 24 (SEQ ID NO 35)
  • the invention provides a variant 6G4.2.5 humanized antibody or antibody fragment comprising a 6G4.2.5LV CDRs variant (here referred to as
  • the invention provides a variant 6G4.2.5 humanized antibody or antibody fragment comprising a 6G4.2.5LV CDRs variant (here referred to as "6G4.2.5LV/L1S26A,N35A/L3H98A") wherein LI corresponds to amino acids 24-39 of the amino acid sequence of Fig. 24 (SEQ ID NO: 35) with the proviso that Ala is substituted for Ser at amino acid position 26 and Ala is substituted for Asn at amino acid position 35, L2 corresponds to amino acids 55-61 of the amino acid sequence of Fig.
  • the humanized light chain variable domains of the invention can be constructed by using any of the techniques for antibody humanization known in the art.
  • the humanized light chain variable domain comprising the CDRs of 6G4.2.5LV or the CDRs of a 6G4.2.5LV CDRs variant can also contain some FR residues that are substituted by residues from analogous sites in the murine 6G4.2.5 antibody light chain variable domain ("6G4.2.5LV").
  • the complete amino acid sequence of 6G4.2.5LV is set out as amino acids 1-1 14 of the amino acid sequence of Fig. 24 (SEQ ID NO: 35).
  • the invention further provides a humanized antibody or antibody fragment comprising a humanized light chain variable domain comprising the CDRs of 6G4.2.5LV or the CDRs of a 6G4.2.5LV CDRs variant as described above, and further comprising a humanized heavy chain variable domain comprising the complementarity determining regions (CDRs) HI , H2, and H3 of the 6G4.2.5 (murine monoclonal antibody) variable heavy chain domain amino acid sequence of Fig. 25 (SEQ ID NO: 37), wherein HI correspond to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37), wherein H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig.
  • CDRs complementarity determining regions
  • the invention provides a humanized antibody or antibody fragment comprising a humanized light chain variable domain comprising the CDRs of 6G4.2.5LV or the CDRs of a 6G4.2.5LV CDRs variant as described above, and further comprising _ ⁇ humanized heavy chain variable domain comprising a variant (herein referred to as a "6G4.2.5HV CDRs variant") ofthe HI , H2, and H3 CDRs of the 6G4.2.5 (murine monoclonal antibody) variable heavy ch_.in domain amino acid sequence of Fig. 25
  • HI correspond to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as "Z3 ] ") is substituted for Ser at amino acid position 31, H2 corresponds to amino acids 50-66 ofthe amino acid sequence of Fig. 25 (SEQ ID NO: 37), and H3 corresponds to amino acids 99-1 11 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37).
  • HI correspond to amino acids 26-35 ofthe amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Ala is substituted for Ser at amino acid position 31, H2 corresponds to amino acids 50-66 ofthe amino acid sequence of Fig. 25 (SEQ ID NO: 37), and H3 corresponds to amino acids 99-11 1 ofthe amino acid sequence of Fig. 25 (SEQ ID NO: 37).
  • HI corresponds to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37), H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig.
  • HI corresponds to ammo acids 26-35 of the ammo acid sequence of Fig 25 (SEQ ID NO 37)
  • H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig 25 (SEQ ID NO 37) with the proviso that Ala is substituted for Ser at amino acid position 54
  • H3 corresponds to ammo acids 99-1 1 1 ofthe amino acid sequence of Fig 25 (SEQ ID NO 37)
  • 6G4 2 5HV CDRs variant referred to herein as "6G4 2 5HV/H3D100E"
  • HI correspond to amino acids 26-35 of the amino acid sequence of Fig 25 (SEQ ID NO 37)
  • H2 corresponds to amino acids 50-66 of the ammo acid sequence of Fig 25 (SEQ ID NO 37)
  • H3 corresponds to amino acids 99-11 1 of the amino acid sequence of Fig 25 (SEQ ID NO 37) with the proviso that Glu is substituted for Asp at ammo acid position 100
  • 6G4 2 5HV CDRs variant (referred to herein as "6G4 2 5HV/H3R102K"), wherein HI correspond to ammo acids 26-35 of the ammo acid sequence of Fig 25 (SEQ ID NO 37), wherein H2 corresponds to ammo acids 50-66 of the amino acid sequence of Fig 25 (SEQ ID NO 37), and wherein H3 corresponds to ammo acids 99-1 1 1 of the amino acid sequence of Fig 25 (SEQ ID NO 37) with the proviso that Lys is substituted for Arg at amino acid position 102
  • 6G4 2 5HV CDRs variant (referred to herein as "6G4 2 5HV H3D106E"), wherein HI correspond to amino acids 26-35 of the ammo acid seque- e of Fig 25 (SEQ ID NO 37), wherein H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig 25 (SEQ ID NO 37), and wherein H3 corresponds to ammo acids 99-11 1 of the amino acid sequence of Fig 25 (SEQ ID NO 37) with the proviso that Glu is substituted for Asp at ammo acid position 106
  • 6G4 2 5HV CDRs variant referred to herein as "6G4 2 5HV/H3D100E.R102K"
  • HI correspond to ammo acids 26-35 of the ammo acid sequence of Fig 25 (SEQ ID NO 37)
  • H2 corresponds to ammo acids 50-66 of the amino acid sequence of Fig 25 (SEQ ID NO 37)
  • H3 corresponds to amino acids 99-1 1 1 of the amino acid sequence of Fig 25 (SEQ ID NO 37) with the proviso that Glu is substituted for Asp at am o acid position 100 and Lys is substituted for Arg at amino acid position 102
  • 6G4 2 5HV CDRs variant (referred to herein as "6G4 2 5HV/H3R102K,D106E"), wherein HI correspond to amino acids 26-35 of the amino acid sequence of Fig 25 (SEQ ID NO 37), wherein H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig 25 (SEQ ID NO 37), and wherein H3 corresponds to amino acids 99-1 11 ofthe amino acid sequence of Fig 25 (SEQ ID NO 37) with the proviso that Lys is substituted for Arg at amino acid position 102 and Glu is substituted for Asp at amino acid position 106
  • 6G4 2 5HV CDRs variant (referred to herein as "6G4 2 5HV/H3D100E,D106E"), wherein HI correspond to ammo acids 26-35 of the amino acid sequence of Fig 25 (SEQ ID NO 37), wherein H2 corresponds to amino acids 50-66 of the ammo acid sequence of Fig 25 (SEQ ID NO 37), and wherein H3 corresponds to amino acids 99-11 1 of the amino acid sequence of Fig 25 (SEQ ID NO 37) with the proviso that Glu is substituted for Asp at amino acid position 100 and Glu is substituted for Asp at ammo acid position 106.
  • HI correspond to ammo acids 26-35 of the amino acid sequence of Fig 25 (SEQ ID NO 37)
  • H2 corresponds to amino acids 50-66 of the ammo acid sequence of Fig 25 (SEQ ID NO 37)
  • H3 corresponds to amino acids 99-11 1 of the amino acid sequence of Fig 25 (SEQ ID NO 37) with the proviso
  • 6G4.2.5HV CDRs variant (referred to herein as "6G4.2.5HV/H3D100E,R102K,D106E")
  • HI correspond to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37)
  • H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37)
  • H3 corresponds to amino acids 99-111 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Glu is substituted for Asp at amino acid position 100, Lys is substituted for Arg at amino acid position 102, and Glu is substituted for Asp at amino acid position 106.
  • HI correspond to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as "Z3 ] ") is substituted for Ser at amino acid position 31, H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as "Z54”) is substituted for Ser at amino acid position 54, and H3 corresponds to amino acids 99-11 1 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37).
  • HI correspond to amino acids 26-35 ofthe amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Ala is substituted for Ser at amino acid position 31
  • H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Ala is substituted for Ser at amino acid position 54
  • H3 corresponds to amino acids 99-1 1 1 ofthe amino acid sequence of Fig. 25 (SEQ ID NO: 37).
  • HI correspond to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as “Z 31 ") is substituted for Ser at amino acid position 31, H2 corresponds to amino acids 50-66 ofthe amino acid sequence of Fig. 25 (SEQ ID NO: 37), and H3 corresponds to amino acids 99- 1 11 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Glu is substituted for Asp at amino acid position 100.
  • Ir a preferred 6G4.2.5HV CDRs variant (referred to herein as "6G4.2.5HV/H1S31A/H3D100E")
  • HI correspond to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Ala is substituted for Ser at amino acid position 31
  • H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37)
  • H3 corresponds to amino acids 99-1 11 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Glu is substituted for Asp at amino acid position 100.
  • 6G4.2.5HV CDRs variant referred to herein as "6G4.2.5HV/H1S31Z 31 H3R102K"
  • HI correspond to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as "Z 31 ") is substituted for Ser at amino acid position 31, H2 corresponds to amino acids 50-66 ofthe amino acid sequence of Fig. 25 (SEQ ID NO: 37), and H3 corresponds to amino acids 99-11 1 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Lys is substituted for Arg at amino acid position 102.
  • a preferred 6G4.2.5HV CDRs variant referred to herein as
  • HI correspond to amino acids 26-35 ofthe amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Ala is substituted for Ser at amino acid position 31
  • H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37)
  • H3 corresponds to amino acids 99-11 1 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Lys is substituted for Arg at amino acid position 102.
  • 6G4.2.5HV CDRs variant (referred to herein as "6G4.2.5HV/H1S31Z 31 /H3D106E”)
  • HI correspond to amino acids 26-35 ofthe amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as "Z 3
  • H2 corresponds to amino acids 50-66 ofthe amino acid sequence of Fig. 25 (SEQ ID NO: 37)
  • H3 corresponds to amino acids 99-1 1 1 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Glu is
  • HI correspond to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Ala is substituted for Ser at amino acid position 31
  • H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37)
  • H3 corresponds to amino acids 99-1 1 1 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Glu is substituted
  • HI correspond to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as "Z 31 ") is substituted for Ser at amino acid position 31
  • H2 corresponds to amino acids 50-66 of the amino acid sequence 20 of Fig. 25 (SEQ ID NO: 37)
  • H3 corresponds to amino acids 99-11 1 ofthe amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Glu is substituted for Asp at amino acid position 100 and Lys is substituted for Arg at amino acid position 10?
  • HI correspond to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Ala is substituted for Ser at amino acid position 31
  • H2 25 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37)
  • H3 corresponds to amino acids 99-1 1 1 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Glu is substituted for Asp at amino acid position 100 and Lys is substituted for Arg at amino acid position 102.
  • HI correspond to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as "Z. , ⁇ ") is substituted for Ser at amino acid position 31, H2 corresponds to amino acids 50-66 ofthe amino acid sequence of Fig. 25 (SEQ ID NO: 37), and H3 corresponds to amino acids 99-1 1 1 ofthe amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Lys is substituted for Arg at amino acid position 102 and Glu is 35 substituted for Asp at amino acid position 106.
  • HI correspond to amino acids 26-35 ofthe amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Ala is substituted for Ser at amino acid position 31
  • H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37)
  • H3 corresponds to amino acids 99-11 1 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Lys is substituted for Arg at amino acid position 102 and Glu is substituted for Asp at amino acid position 106.
  • HI correspond to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as "Z3J") is substituted for Ser at amino acid position 31, H2 corresponds to amino acids 50-66 ofthe amino acid sequence of Fig. 25 (SEQ ID NO: 37), and H3 corresponds to amino acids 99-11 1 ofthe amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Glu is substituted for Asp at amino acid position 100 and Glu is substituted for Asp at amino acid position 106.
  • HI correspond to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Ala is substituted for Ser at amino acid position 31
  • H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37)
  • H3 corresponds to amino acids 99-111 ofthe amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Glu is substituted for Asp at amino acid position 100 and Glu is substituted for Asp at amino acid position 106.
  • HI correspond to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as "Z31") is substituted for Ser at amino acid position 31, H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37), and H3 corresponds to amino acids 99-1 1 1 ofthe amino acid sequence of Fig.
  • HI correspond to amino acids 26-35 ofthe amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Ala is substituted for Ser at amino acid position 31, H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig.
  • H3 corresponds to amino acids 99-111 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Glu is substituted for Asp at amino acid position 100, Lys is substituted for Arg at amino acid position 102 and Glu is substituted for Asp at amino acid position 106.
  • 6G4.2.5HV CDRs variant referred to herein as "6G4.2.5HV/H2S54Z 54 /H3D100E"
  • HI corresponds to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37)
  • H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as "Z 54 ") is substituted for Ser at amino acid position 54
  • H3 corresponds to amino acids 99-1 1 1 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Glu is substituted for Asp at amino acid position 100.
  • HI corresponds to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37)
  • H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Ala is substituted for Ser at amino acid position 54
  • H3 corresponds to amino acids 99-11 1 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Glu is substituted for Asp at amino acid position 100.
  • 6G4.2.5HV CDRs variant referred to herein as "6G4.2.5HV/H2S54Z 54 /H3R102K"
  • HI corresponds to amino acids 26-35 ofthe amino acid sequence of Fig. 25 (SEQ ID NO: 37)
  • H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as "Z 54 ") is substituted for Ser at amino acid position 54
  • H3 corresponds to amino acids 99-11 1 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Lys is substituted for Arg at amino acid position 102.
  • HI corresponds to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37)
  • H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Ala is substituted for Ser at amino acid position 54
  • H3 corresponds to amino acids 99-1 1 1 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Lys is substituted for Arg at arrino acid position 102.
  • HI corresponds to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37)
  • H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as “Z5 4 ”) is substituted for Ser at amino acid position 54
  • H3 corresponds to amino acids 99-11 1 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Glu is substituted for Asp at amino acid position 106.
  • HI corresponds to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37)
  • H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Ala is substituted for Ser at amino acid position 54
  • H3 corresponds to amino acids 99-11 1 ofthe amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Glu is substituted for Asp at amino acid position 106.
  • a twenty-second 6G4.2.5HV CDRs variant referred to herein as
  • HI corresponds to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37), H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25
  • HI corresponds to amino acids 26-35 of the amino acid sequence of Fig.
  • H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Ala is substituted for Ser at amino acid position 54
  • H3 corresponds to amino acids 99-1 11 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Glu is substituted for Asp at amino acid position 100 and Lys is substituted for Arg at amino acid position 102.
  • HI corresponds to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37)
  • H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as “Z 5 ”) is substituted for Ser at amino acid position 54
  • H3 corresponds to amino acids 99-1 11 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Lys is substituted for Arg at amino acid position 102 and Glu is substituted for Asp at amino acid position 106.
  • HI corresponds to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37)
  • H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Ala is substituted for Ser at amino acid position 54
  • H3 corresponds to amino acids 99-1 1 1 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Lys is substituted for Arg at amino acid position 102 and Glu is substituted for Asp at amino acid position 106.
  • 6G4.2.5HV CDRs variant referred to herein as "6G4.2.5HV/H2S54A/H3R102K,D106E”
  • HI corresponds to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37), H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25
  • HI corresponds to amino acids 26-35 of the amino acid sequence of Fig.
  • H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Ala is substituted for Ser at amino acid position 54
  • H3 corresponds to amino acids 99-111 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Glu is substituted for Asp at amino acid position 100 and Glu is substituted for Asp at amino acid position 106.
  • HI corresponds to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37), H2 corresponds to amino acids 50-66 of the amino acid sequence of
  • Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as “Z 5 ”) is substituted for Ser at amino acid position 54
  • H3 corresponds to amino acids 99-111 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Glu is substituted for Asp at amino acid position 100
  • Lys is substituted for Arg at amino acid position 102
  • Glu is substituted for Asp at amino acid position 106.
  • HI corresponds to amino acids 26-35 of the amino acid sequence of Fig.
  • H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Ala is substituted for Ser at amino acid position 54
  • H3 corresponds to amino acids 99-1 1 1 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Glu is substituted for Asp at amino acid position 100
  • Lys is substituted for Arg at amino acid position 102
  • Glu is substituted for Asp at amino acid position 106.
  • HI correspond to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as "Z 3 ⁇ ") is substituted for.
  • Ser at amino acid position 31 H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as "Z 54 ") is substituted for Ser at amino acid position 54
  • H3 corresponds to amino acids 99-1 1 1 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Glu is substituted for Asp at amin acid position 100.
  • 6G4.2.5HV CDRs variant referred to herein as
  • HI correspond to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Ala is substituted for Ser at amino acid position 31
  • H2 corresponds to amino acids 50-66 ofthe amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Ala is substituted for Ser at amino acid position 54
  • H3 corresponds to amino acids 99-1 11 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Glu is substituted for Asp at amino acid position 100.
  • 6G4.2.5HV CDRs variant referred to herein as
  • HI correspond to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as "Z 31 ") is substituted for Ser at amino acid position 31
  • H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as "Z 54 ") is substituted for Ser at amino acid position 54
  • H3 corresponds to amino acids 99-1 1 1 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Lys is substituted for Arg at amino acid position 102.
  • 6G4.2.5HV CDRs variant referred to herein as
  • HI correspond to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Ala is substituted for Ser at amino acid position 31
  • H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Ala is substituted for Ser at amino acid position 54
  • H3 corresponds to amino acids 99-11 1 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Lys is substituted for Arg at amino acid position 102.
  • HI correspond to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as "Z 31 ") is substituted for Ser at amino acid position 31
  • H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as "Z 54 ") is substituted for Ser at amino acid position 54
  • H3 corresponds to amino acids 99-1 1 1 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Glu is substituted for Asp at amino acid position 106.
  • 6G4.2.5HV CDRs variant referred to herein as
  • HI correspond to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Ala is substituted for Ser at amino acid position 31
  • H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Ala is substituted for Ser at amino acid position 54
  • H3 corresponds to amino acids 99-1 1 1 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Glu is substituted for Asp at amino acid position 106.
  • HI correspond to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as "Z 31 ") is substituted for Ser at amino acid position 31, H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as "Z 54 ") is substituted for Ser at amino acid position 54, and H3 corresponds to amino acids 99-1 1 1 of the amino acid sequence of Fig.
  • HI correspond to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Ala is substituted for Ser at amino acid position 31, H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig.
  • HI correspond to amino acids 26-35 ofthe amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as "Z 31 ") is substituted for Ser at amino acid position 31, H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as "Z 5 ") is substituted for Ser at amino acid position 54, and H3 corresponds to amino acids 99-1 1 1 of the amino acid sequence of Fig.
  • HI correspond to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Ala is substituted for Ser at amino acid position 31, H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig.
  • HI correspond to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as "Z 31 ") is substituted for Ser at amino acid position 31, H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as "Z 54 ") is substituted for Ser at amino acid position 54, and H3 corresponds to amino acids 99-111 of the amino acid sequence of Fig.
  • H3 corresponds to amino acids 99-1 1 1 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Glu is substituted for Asp at amino acid position 100 and Glu is substituted for Asp at amino acid position 106.
  • HI correspond to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as "Z 31 ") is substituted for Ser at amino acid position 31, H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that any amino acid other than Ser (denoted as
  • Z 5 is substituted for Ser at amino acid position 54, and H3 corresponds to amino acids 99-11 1 ofthe amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Glu is substituted for Asp at amino acid position 100, Lys is substituted for Arg at amino acid position 102 and Glu is substituted for Asp at amino acid position 106.
  • a preferred 6G4.2.5HV CDRs variant referred to herein as
  • HI correspond to amino acids 26-35 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Ala is substituted for Ser at amino acid position 31
  • H2 corresponds to amino acids 50-66 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Ala is substituted for Ser at amino acid position 54
  • H3 corresponds to amino acids 99-1 11 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37) with the proviso that Glu is substituted for Asp at amino acid position 100
  • Lys is substituted for Arg at amino acid position 102
  • Glu is substituted for Asp at amino acid position 106.
  • a humanized heavy chain variable domain is constructed by substituting the CDRs of 6G4.2.5HV or the CDRs of a 6G4.2.5HV CDRs variant for the corresponding sequences in a human heavy chain variable domain.
  • the humanized heavy chain variable domain comprising the CDRs of 6G4.2.5HV or the CDRs of a 6G4.2.5HV CDRs variant can also contain some FR residues that are substituted by residues from analogous sites in the murine 6G4.2.5 antibody heavy chain variable domain.
  • the complete amino acid sequence of 6G4.2.5HV is set out as amino acids 1- 122 of the amino acid sequence of Fig. 25 (SEQ ID NO: 37).
  • variable domains both light and heavy
  • the choice of human variable domains, both light and heavy, to be used in making the humanized antibodies and antibody fragments is very important to reduce antigenicity.
  • sequence of the variable domain of a rodent antibody is screened against the entire library of known human variable-domain sequences.
  • the human sequence which is closest to that of the rodent is then accepted as the human framework (FR) for the humanized antibody (Sims et al, J. Immunol. 151 : 2296 (1993); Chothia and Lesk, J. Mol. Biol. 196:901 (1987)).
  • Another method uses a particular framework derived from the consensus sequence of all human antibodies of a particular subgroup of light or heavy chains.
  • the same framework can be used for several different humanized antibodies (Carter et al, Proc. Natl. Acad. Sci. U.S.A. 89:4285 (1992); Presta et al, J. Immunol. 151:2623 (1993)). It is also important that the antibodies and antibody fragments of the invention be humanized with retention of high affinity for human IL-8 and other favorable biological properties. To achieve this goal, according to a preferred method, the humanized antibodies and antibody fragments of the invention are prepared by a process of analysis of the parental sequences and various conceptual humanized products using three-dimensional models of the parental and humanized sequences. Three dimensional immunoglobulin models are commonly available and are familiar to those skilled in the art.
  • any and all humanized light chain variable domain amino acid sequences which comprise the CDRs of 6G4.2.5LV/L3H98A are collectively referred to herein as "hu6G4.2.5LV/L3H98A”.
  • Any and all humanized light chain variable domain amino acid sequences which comprise the CDRs of 6G4.2.5LV/L1S26X 26 ,N35X 35 are collectively referred to herein as "hu6G4.2.5LV/LlS26X 26 ,N35X 35 ".
  • the humanized light chain variable domain amino acid sequences of hu6G4.2.5LV/LlN35A, hu6G4.2.5LV/LlS26A, hu6G4.2.5LV/LlS26A/L3H98A, hu6G4.2.5LV/LlS26A,N35A, hu6G4.2.5LV/LlN35A/L3H98A, hu6G4.2.5LV/LlS26A/L3H98A, hu6G4.2.5LV/Ll S26A,N35A/L3H98A are collectively referred to herein as "hu6G4.2.5LV/vLl-3A".
  • any and all humanized heavy chain variable domain amino acid sequences which comprise the CDRs of 6G4.2.5HV H1 S31A are collectively referred to herein as "hu6G4.2.5HV/HlS31A".
  • Any and all humanized heavy chain variable domain amino acid sequences which comprise the CDRs of 6G4.2.5HV/H2S54Z 54 are collectively referred to herein as "hu6G4.2.5HV H2S54Z 54 ".
  • any and all humanized heavy chain variable domain amino acid sequences which comprise the CDRs of 6G4.2.5HV/H3D106E are collectively referred to herein as "hu6G4.2.5HV/H3D106E”.
  • Any and all humanized heavy chain variable domain amino acid sequences which comprise the CDRs of 6G4.2.5HV/H3D100E.R102K are collectively referred to herein as "hu6G4.2.5HV/H3D100E,R102K”.
  • any and all humanized heavy chain variable domain amino acid sequences which comprise the CDRs of 6G4.2.5HV/H1S31A/H3R102K are collectively referred to herein as "hu6G4.2.5HV/HlS31A/H3R102K”.
  • Any and all humanized heavy chain variable domain amino acid sequences which comprise the CDRs of 6G4.2.5HV/H1S31A/H3D106E are collectively referred to herein as "hu6G4.2.5HV/HlS31A/H3D106E".
  • any and all humanized heavy chain variable domain amino acid sequences which comprise the CDRs of 6G4.2.5HV/H1S31A/H2S54A/H3D100E are collectively referred to herein as "hu6G4.2.5HV/HlS31A/H2S54A/H3D100E".
  • Any and all humanized heavy chain variable domain amino acid sequences which comprise the CDRs of 6G4.2.5HV/H1 S31A/H2S54A/H3R102K are collectively referred to herein as "hu6G4.2.5HV/HlS31A/H2S54A/H3R102K".
  • the invention provides a humanized antibody or antibody fragment that comprises a light chain variable domain comprising the hu6G4.2.5LV/vLl-3X. In another embodiment, the invention provides a humanized antibody or antibody fragment that comprises a light chain variable domain comprising the hu6G4.2.5LV/vLl-3A. In yet another embodiment, the invention provides a humanized antibody or antibody fragment that comprises a light chain variable domain comprising the hu6G4.2.5LV/LlN35X 35 . In still another embodiment, the invention provides a humanized antibody or antibody fragment that comprises a light chain variable domain comprising the hu6G4.2.5LV/LlN35A. In a further embodiment, the invention provides a humanized antibody or antibody fragment that comprises a light chain variable domain comprising the hu6G4.2.5LV/LlN35E.
  • the invention additionally provides a humanized antibody or antibody fragment that comprises a light chain variable domain comprising the hu6G4.2.5LV/vLl-3X, and further comprises a heavy chain variable domain comprising th hu6G4.2.5HV or hu6G4.2.5HV/vHl-3Z.
  • the inventior provides a humanized antibody or antibody fragment that comprises a light chain variable domain comprising the hu6G4.2.5LV/vLl-3A, and further comprises a heavy chain variable domain comprising the hu6G4.2.5HV or hu6G4.2.5HV/vHl-3Z.
  • the invention provides a humanized antibody or antibody fragment that comprises a light chain variable domain comprising the hu6G4.2.5LV/vLl-3A, and further comprises a heavy chain variable domain comprising the hu6G4.2.5HV/vHl-3A.
  • the invention provides a humanized antibody or antibody fragment that comprises a light chain variable domain comprising the hu6G4.2.5LV/LlN35X 35 , and further comprises a heavy chain variable domain comprising the hu6G4.2.5HV or hu6G4.2.5HV/vHl-3Z.
  • the invention provides a humanized antibody or antibody fragment that comprises a light chain variable domain comprising the hu6G4.2.5LV/N35X 35 , and further comprises a heavy chain variable domain comprising the hu6G4.2.5HV/vHl-3A.
  • the antibody or antibody fragment comprises a light chain variable domain comprising the hu6G4.2.5LV/LlN35X 35 and further comprises a humanized heavy chain comprising the amino acid sequence of 6G4.2.5HV11.
  • the invention provides a humanized antibody or antibody fragment that comprises a light chain variable domain comprising the hu6G4.2.5LV/LlN35A, and further comprises a heavy chain variable domain comprising the hu6G4.2.5HV or hu6G4.2.5HV/vHl-3Z.
  • the invention provides a humanized antibody or antibody fragment that comprises a light chain variable domain comprising the hu6G4.2.5LV/N35A, and further comprises a heavy chain variable domain comprising the hu6G4.2.5HV/vHl-3A.
  • the humanized antibody or antibody fragment comprises a light chain variable domain comprising the hu6G4.2.5LV/LlN35A, and further comprises a heavy chain variable domain comprising the hu6G4.2.5HV.
  • the humanized antibody or antibody fragment comprises a light chain variable domain comprising the hu6G4.2.5LV/LlN35E, and further comprises a heavy chain variable domain comprising the hu6G4.2.5HV.
  • the antibody or antibody fragment comprises a light chain variable domain comprising the hu6G4.2.5LV/LlN35A and further comprises a humanized heavy chain comprising the amino acid sequence of 6G4.2.5HV11.
  • the antibody or antibody fragment comprises a light chain variable domain comprising the hu6G4.2.5LV/LlN35E and further comprises a humanized heavy chain comprising the amino acid sequence of 6G4.2.5HV1 1.
  • the invention encompasses a single chain antibody fragment comprising the hu6G4.2.5LV/vLl-3X, with or without any additional amino acid sequence.
  • the invention provides a single chain antibody fragment comprising the hu6G4.2.5LV/vLl-3X without any associated heavy chain variable domain amino acid sequence, i.e. a single chain species that makes up one half of an Fv fragment.
  • the invention provides a single chain antibody fragment comprising the hu6G4.2.5LV/vLl-3A without any associated heavy chain variable domain amino acid sequence. In still another embodiment, the invention provides a single chain antibody fragment comprising the hu6G4.2.5LV/LlN35X 3 5 without any associated heavy chain variable domain amino acid sequence. In a preferred embodiment, the invention provides a sing't chain antibody fragment comprising the hu6G4.2.5LV/LlN35A without any asset iated heavy chain variable domain amino acid sequence. In another preferred embodiment, the invention provides a single chain antibody fragment comprising the hu6G4.2.5LV/LlN35E without any associated heavy chain variable domain amino acid sequence.
  • the invention provides a single chain antibody fragment wherein the hu6G4.2.5LV/vLl-3X and the hu6G4.2.5HV or hu6G4.2.5HV/vHl-3Z are contained in a single chain polypeptide species.
  • the single chain antibody fragment is a scFv species comprising the hu6G4.2.5LV/vLl-3X joined to the hu6G4.2.5HV or hu6G4.2.5HV/vHl-3Z by means of a flexible peptide linker sequence, wherein the heavy chain and light chain variable domains can associate in a "dimeric" structure analogous to that formed in a two-chain Fv species.
  • the single chain antibody fragment is a species comprising the hu6G4.2.5LV/vLl-3X joined to the hu6G4.2.5HV or hu6G4.2.5HV/vHl-3Z by a linker that is too short to permit intramolecular pairing of the two variable domains, i.e. a single chain polypeptide monomer that forms a diabody upon dimerization with another monomer.
  • the invention provides a single chain antibody fragment wherein the hu6G4.2.5LV/vLl-3A and the hu6G4.2.5HV or hu6G4.2.5HV/vHl-3Z are contained in a single chain polypeptide species.
  • the single chain antibody fragment is a scFv species comprising the hu6G4.2.5LV/vLl-3A joined to the hu6G4.2.5HV or hu6G4.2.5HV/vHl-3Z by means of a flexible peptide linker sequence, wherein the heavy chain and light chain variable domains can associate in a "dimeric" structure analogous to that formed in a two-chain Fv species.
  • the single chain antibody fragment is a species comprising the hu6G4.2.5LV/vLl-3A joined to the hu6G4.2.5HV or hu6G4.2.5HV/vHl-3Z by a linker that is too short to permit intramolecular pairing of the two variable domains, i.e. a single chain polypeptide monomer that forms a diabody upon dimerization with another monomer.
  • the invention provides a single chain antibody fragment wherein the hu6G4.2.5LV/vLl-3A and the hu6G4.2.5HV/vHl-3A are contained in a single chain polypeptide species.
  • the single chain antibody fragment is a scFv species comprising the hu6G4.2.5LV/vLl- 3A joined to the hu6G4.2.5HV/vHl-3A by means of a flexible peptide linker sequence, wherein the heavy chain and light chain variable domains can associate in a "dimeric" structure analogous to that formed in a two- chain Fv species.
  • the single chain antibody fragment is a species comprising the hu6G4.2.5LV/vLl-3A joined to the hu6G4.2.5HV/vHl-3A by a linker that is too short to permit intramolecular pairing of the two variable domains, i.e. a single chain polypeptide monomer that forms a diabody upon dimerization with another monomer.
  • the invention provides a single chain antibody fragment wherein the hu6G4.2.5LV/LlN35X 35 and the hu6G4.2.5HV or hu6G4.2.5HV/vHl-3Z are contained in a single chain polypeptide species.
  • the single chain antibody fragment is a scFv species comprising the hu6G4.2.5LV/LlN35X 35 joined to the hu6G4.2.5HV or hu6G4.2.5HV/vHl-3Z by means of a flexible peptide linker sequence, wherein the heavy chain and light chain variable domains can associate in a "dimeric" structure analogous to that formed in a two-chain Fv species.
  • the single chain antibody fragment is a species comprising the hu6G4.2.5LV LlN35X 3 5 joined to the hu6G4.2.5HV or hu6G4.2.5HV/vHl-3Z by a linker that is too short to permit intramolecular pairing of the two variable domains, i.e. a single chain polypeptide monomer that forms a diabody upon dimerization with another monomer.
  • the invention provides a single chain antibody fragment wherein the hu6G4.2.5LV/LlN35X 35 and the hu6G4.2.5HV/vHl-3A are contained in a single chain polypeptide species.
  • the single chain antibody fragment is a scFv species comprising the hu6G4.2.5LV/LlN35X 35 joined to the hu6G4.2.5HV/vHl-3A by means of a flexible peptide linker sequence, wherein the heavy chain and light chain variable domains can associate in a "dimeric" structure analogous to that formed in a two-chain Fv species.
  • the single chain antibody fragment is a species comprising the hu6G4.2.5LV/LlN35X 35 joined to the hu6G4.2.5HV/vHl-3A by a linker that is too short to permit intramolecular pairing of the two variable domains, i.e. a single chain polypeptide monomer that forms a diabody upon dimerization with another monomer.
  • the invention provides a single chain antibody fragment wherein the hu6G4.2.5LV/LlN35A and the hu6G4.2.5HV or hu6G4.2.5HV/vHl-3Z are contained in a single chain polypeptide species.
  • the single chain antibody fragment is a scFv species comprising the hu6G4.2.5LV/LlN35A joined to the hu6G4.2.5HV or hu6G4.2.5HV/vHl-3Z by means of a flexible peptide linker sequence, wherein the heavy chain and light chain variable domains can associate in a "dimeric" structure analogous to that formed in a two-chain Fv species.
  • the single chain antibody fragment is a species comprising the hu6G4.2.5LV/LlN35A joined to the hu6G4.2.5HV or hu6G4.2.5HV/vHl-3Z by a linker that is too short to permit intramolecular pairing of the two variable domains, i.e. a single chain polypeptide monomer that forms a diabody upon dimerization with another monomer.
  • the single chain antibody fragment wherein the hu6G4.2.5LV/LlN35E and the hu6G4.2.5HV are contained in a single chain polypeptide species.
  • the single chain antibody fragment is a scFv species comprising the hu6G4.2.5LV/LlN35E joined to the hu6G4.2.5HV by means of a flexible peptide linker sequence, wherein the heavy chain and light chain variable domains can associate in a "dimeric" structure analogous to that formed in a two-chain Fv species.
  • the single chain antibody fragment is a species comprising the hu6G4.2.5LV/LlN35E joined to the hu6G4.2.5HV by a linker that is too short to permit intramolecular pairing of the two variable domains, i.e. a single chain polypeptide monomer that forms a diabody upon dimerization with another monomer.
  • the invention provides a single chain antibody fragment wherein the hu6G4.2.5LV/LlN35A and the hu6G4.2.5HV/vHl-3A are contained in a single chain polypeptide species.
  • the single chain antibody fragment is a scFv species comprising the hu6G4.2.5LV/LlN35A joined to the hu6G4.2.5HV/vHl-3A by means of a flexible peptide linker sequence, wherein the heavy chain and light chain variable domains can associate in a "dimeric" structure analogous to that formed in a two-chain Fv species.
  • the single chain antibody fragment is a species comprising the hu6G4.2.5LV/L!N35A joined to the hu6G4.2.5HV/vHl-3A by a linker that is too short to permit intramolecular pairing of the two variable domains, i.e. a single chain polypeptide monomer that forms a diabody upon dimerization with another monomer.
  • the invention provides an antibody fragment comprising a plurality of polypeptide chains, wherein one polypeptide chain comprises the hu6G4.2.5LV/vLl-3X ai... a second polypeptide chain comprises the hu6G4.2.5HV or hu6G4.2.5HV/vHl-3Z and the two polypeptide chains are CO' alently linked by one or more interchain disulfide bonds.
  • the invention provides an antibody fragment comprising a plurality of polypeptide chains, wherein one polypeptide chain comprises the hu6G4.2.5LV/vLl-3X and a second polypeptide chain comprises the hu6G4.2.5HV/vHl-3A and the two polypeptide chains are covalently linked by one or more interchain disulfide bonds.
  • the invention provides an antibody fragment comprising a plurality of polypeptide chains, wherein one polypeptide chain comprises the hu6G4.2.5LV/vLl-3X and a second polypeptide chain comprises the amino acid sequence of 6G4.2.5HV11 and the two polypeptide chains are covalently linked by one or more interchain disulfide bonds.
  • the invention provides an antibody fragment comprising a plurality of polypeptide chains, wherein one polypeptide chain comprises the hu6G4.2.5LV/vLl-3A and a second polypeptide chain comprises the hu6G4.2.5HV or hu6G4.2.5HV/vHl-3Z and the two polypeptide chains are covalently linked by one or more interchain disulfide bonds.
  • the invention provides an antibody fragment comprising a plurality of polypeptide chains, wherein one polypeptide chain comprises the hu6G4.2.5LV/vLl-3A and a second polypeptide chain comprises the hu6G4.2.5HV/vHl-3A and the two polypeptide chains are covalently linked by one or more interchain disulfide bonds.
  • the invention provides an antibody fragment comprising a plurality of polypeptide chains, wherein one polypeptide chain comprises the hu6G4.2.5LV/vLl-3A and a second polypeptide chain comprises the amino acid sequence of 6G4.2.5HV11 and the two polypeptide chains are covalently linked by one or more interchain disulfide bonds.
  • the invention also encompasses an antibody fragment comprising a plurality of polypeptide chains, wherein one polypeptide chain comprises the hu6G4.2.5LV/LlN35X 35 and a second polypeptide chain comprises the hu6G4.2.5HV or hu6G4.2.5HV/vHl-3Z and the two polypeptide chains are covalently linked by one or more interchain disulfide bonds.
  • the invention provides an antibody fragment comprising a plurality of polypeptide chains, wherein one polypeptide chain comprises the hu6G4.2.5LV/LlN35X 35 and a second polypeptide chain comprises the hu6G4.2.5HV/vHl-3A and the two polypeptide chains are covalently linked by one or more interchain disulfide bonds.
  • the invention provides an antibody fragment comprising a plurality of polypeptide chains, wherein one polypeptide chain comprises the hu6G4.2.5LV LlN35X 35 and a second polypeptide chain comprises the amino acid sequence of 6G4.2.5HV11 and the two polypeptide chains are covalently linked by one or more interchain disulfide bonds.
  • the invention further encompasses an antibody fragment comprising a plurality of polypeptide chains,
  • one polypeptide chain comprises the hu6G4.2.5LV/LlN35A and a second polypeptide chain comprises the hu6G4.2.5HV or hu6G4.2.5HV/vHl-3Z and the two polypeptide chains are covalently linked by one or more interchain disulfide bonds.
  • the invention also encompasses an antibody fragment comprising a plurality of polypeptide chains, wherein one polypeptide chain comprises the hu6G4.2.5LV/LlN35E and a second polypeptide chain comprises the hu6G4.2.5HV and the two polypeptide chains are covalently linked by one or more interchain disulfide bonds.
  • the invention provides an antibody fragment comprising a plurality of polypeptide chains, wherein one polypeptide chain comprises the hu6G4.2.5LV/LlN35A and a second polypeptide chain comprises the hu6G4.2.5HV/vHl-3A and the two polypeptide chains are covalently linked by one or more interchain disulfide bonds.
  • the invention provides an antibody fragment comprising a plurality of polypeptide chains, wherein one polypeptide chain comprises the hu6G4.2.5LV/LlN35A and a second polypeptide chain comprises the amino acid sequence of 6G4.2.5HV11 and the two polypeptide chains are covalently linked by one or more interchain disulfide bonds.
  • the invention provides an antibody fragment comprising a plurality of polypeptide chains, wherein one polypeptide chain comprises the hu6G4.2.5LV/LlN35E and a second polypeptide chain comprises the amino acid sequence of 6G4.2.5HV1 1 and the two polypeptide chains are covalently linked by one or more interchain disulfide bonds.
  • any of the foregoing two-chain antibody fragments are selected from the group consisting of Fab, Fab', Fab'-SH, Fv, and F(ab') 2 .
  • the antibody fragment is selected from the group consisting of Fab, Fab', Fab'-SH, Fv, and F(ab') 2 , wherein the antibody fragment comprises one polypeptide chain comprising the hu6G4.2.5LV/LlN35X3 5 and a second polypeptide chain comprising the hu6G4.2.5HV.
  • the antibody fragment is selected from the group consisting of Fab, Fab', Fab'-SH, Fv, and F(ab') 2 , wherein the antibody fragment comprises one polypeptide chain comprising the hu6G4.2.5LV/LlN35A and a second polypeptide chain comprising the hu6G4.2.5HV.
  • the antibody fragment is selected from the group consisting of Fab, Fab', Fab'-SH, Fv, and F(ab') 2 , wherein the antibody fragment comprises one polypeptide chain comprising the hu6G4.2.5LV/LlN35E and a second polypeptide chain comprising the hu6G4.2.5HV.
  • the antibody fragment is a F(ab') 2 that comprises one polypeptide chain comprising the hu6G4.2.5LV/LlN35A and a second polypeptide chain comprising the amino acid sequence of
  • the antibody fragment is a F(ab') 2 that comprises one polypeptide chain comprising the hu6G4.2.5LV/LlN35E and a second polypeptide chain comprising the amino acid sequence of 6G4.2.5HV1 1.
  • the invention also provides an antibody or antibody fragment comprising a light chain variable domain containing the hu6G4.2.5LV/vLl-3X and optionally further comprising a heavy chain variable domain containing the hu6G4.2.5HV or hu6G4.2.5HV/vHl-3Z, wherein the ligh' chain variable domain, and optionally the heavy chain variable domain, is (are) fused to an additional moiety, such as a immunoglobulin constant domain.
  • Constant domain sequence can be added to the heavy chain and or light chain sequence(s) to form species with full or partial length heavy and/or light chain(s).
  • constant regions of any isotype can be used for this pu ⁇ ose, including IgG, IgM, IgA, IgD, and IgE constant regions, and that such constant regions can be obtained from any human or animal species.
  • the constant domain sequence is human in origin. Suitable human constant domain sequences can be obtained from Kabat et al.
  • the invention additionally provides an antibody or antibody fragment comprising a light chain variable domain containing the hu6G4.2.5LV/vLl-3X and optionally further comprising a heavy chain variable domain containing the hu6G4.2.5HV/vHl-3A, wherein the light chain variable domain, and optionally the heavy chain variable domain, is (are) fused to an additional moiety, such as a immunoglobulin constant domain.
  • Constant domain sequence can be added to the heavy chain and/or light chain sequence(s) to form species with full or partial length heavy and/or light chain(s).
  • constant regions of any isotype can be used for this pu ⁇ ose, including IgG, IgM, IgA, IgD, and IgE constant regions, and that such constant regions can be obtained from any human or animal species.
  • the constant domain sequence is human in origin. Suitable human constant domain sequences can be obtained from Kabat et al.
  • the invention further provides an antibody or antibody fragment comprising a light chain variable domain containing the hu6G4.2.5LV/LlN35X 35 and optionally further comprising a heavy chain variable domain containing the hu6G4.2.5HV or hu6G4.2.5HV/vHl-3Z, wherein the light chain variable domain, and optionally the heavy chain variable domain, is (are) fused to an additional moiety, such as a immunoglobulin constant domain.
  • Constant domain sequence can be added to the heavy chain and/or light chain sequence(s) to form species with full or partial length heavy and/or light chain(s).
  • constant regions of any isotype can be used for this pu ⁇ ose, including IgG, IgM, IgA, IgD, and IgE constant regions, and that such constant regions can be obtained from any human or animal species.
  • the constant domain sequence is human in origin. Suitable human constant domain sequences can be obtained from Kabat et al.
  • the invention additionally provides an antibody or antibody fragment comprising a light chain variable domain containing the hu6G4.2.5LV/LlN35X 3 5 and optionally further comprising a heavy chain variable domain containing the hu6G4.2.5HV/vHl-3A, wherein the light chain variable domain, and optionally the heavy chain variable domain, is (are) fused to an additional moiety, such as a immunoglobulin constant domain.
  • Constant domain sequence can be added to the heavy chain and/or light chain sequence(s) to form species with full or partial length heavy and or light chain(s).
  • constant regions of any isotype can be used for this pu ⁇ ose, including IgG, IgM, IgA, IgD, and IgE constant regions, and that such constant regions can be obtained from any human or animal species.
  • the constant domain sequence is human in origin. Suitable human constant domain sequences can be obtained from Kabat et al.
  • the invention also encompasses an antibody or antibody fragment comprising a light chain variable domain containing the hu6G4.2.5LV/LlN35A and optionally further comprising a heavy chain variable domain containing the hu6G4.2.5HV or hu6G4.2.5HV/vHl-3Z, wherein the light chain variable domain, and optionally the heavy chain variable domain, is (are) fused to an additional moiety, such as a immunoglobulin constant domain.
  • Constant domain sequence can be added to the heavy c ain and/or light chain sequence(s) to form species with full or partial length heavy and/or light chain(s).
  • constant regions of any isotype can be used for this pu ⁇ ose, including IgG, IgM, IgA, IgD, and IgE constant regions, and that such constant regions can be obtained from any human or animal species.
  • the constant domain sequence is human in origin. Suitable human constant domain sequences can be obtained from Kabat et al.
  • the invention additionally provides an antibody or antibody fragment comprising a light chain variable domain containing the hu6G4.2.5LV/LlN35A and optionally further comprising a heavy chain variable domain containing the hu6G4.2.5HV/vHl-3A, wherein the light chain variable domain, and optionally the heavy chain variable domain, is (are) fused to an additional moiety, such as a immunoglobulin constant domain.
  • Constant domain sequence can be added to the heavy chain and/or light chain sequence(s) to form species with full or partial length heavy and/or light chain(s).
  • constant regions of any isotype can be used for this pu ⁇ ose, including IgG, IgM, IgA, IgD, and IgE constant regions, and that such constant regions can be obtained from any human or animal species.
  • the constant domain sequence is human in origin. Suitable human constant domain sequences can be obtained from Kabat et al.
  • the invention additionally encompasses an antibody or antibody fragment comprising a light chain variable domain containing the hu6G4.2.5LV/LlN35A and optionally further comprising a heavy chain containing the amino acid sequence of 6G4.2.5HV1 1, wherein the light chain variable domain, and optionally the heavy chain, is (are) fused to an additional moiety, such as immunoglobulin constant domain sequences.
  • Constant domain sequence can be added to the heavy chain and/or light chain sequence(s) to form species with full or partial length heavy and/or light chain(s).
  • constant regions of any isotype can be used for this pu ⁇ ose, including IgG, IgM, IgA, IgD, and IgE constant regions, and that such constant regions can be obtained from any human or animal species.
  • the constant domain sequence is human in origin. Suitable human constant domain sequences can be obtained from Kabat et al.
  • the invention further encompasses an antibody or antibody fragment comprising a light chain variable domain containing the hu6G4.2.5LV LlN35E and optionally further comprising a heavy chain containing the amino acid sequence of 6G4.2.5HV1 1, wherein the light chain variable domain, and optionally the heavy chain, is (are) fused to an additional moiety, such as immunoglobulin constant domain sequences.
  • Constant domain sequence can be added to the heavy chain and/or light chain sequence(s) to form species with full or partial length heavy and/or light chain(s).
  • constant regions of any isotype can be used for this pu ⁇ ose, including IgG, IgM, IgA, IgD, and IgE constant regions, and that such constant regions can be obtained from any human or animal species.
  • the constant domain sequence is human in origin. Suitable human constant domain sequences can be obtained from Kabat et al.
  • the antibody or antibody fragment comprises a light chain variable domain containing the hu6G4.2.5LV/vLl-3X, and further comprises the hu6G4.2.5HV or hu6G4.2.5HV/vHl-
  • leucine zipper can increase the affinity or production efficiency of the antibody or antibody fragment of interest.
  • Suitable leucine zipper sequences include the jun and fos leucine zippers taught by Kostelney et al, J. Immunol., 148: 1547-1553
  • the invention provides an antibody or antibody fragment comprising a light chain comprising the amino acid sequence of amino acids 1-219 of th-- variant humanized anti-IL-8 6G4.2.5vl 1 light chain polypeptide amino acid sequence of Fig. 3 IB (SEQ ID NO: 51) with the proviso that any amino acid other than Asn (denoted as "X35”) is substituted for Asn at amino acid position 35 (herein referred to as
  • the invention provides an antibo . or antibody fragment comprising a light chain comprising the amino acid sequence of amino acids 1-219 of the variant humanized anti-IL-8 6G4.2.5vl l light chain polypeptide amino acid sequence of Fig. 3 IB (SEQ ID NO: 51) with the proviso that any amino acid other than Ser (denoted as "X26 ') ⁇ s substituted for Ser at amino acid position 26 (herein referred to as "6G4.2.5LV11S26X 26 ").
  • the invention provides an antibody or antibody fragment comprising a light chain comprising the amino acid sequence of amino acids 1-219 of the variant humanized anti-IL-8 6G4.2.5vl 1 light chain polypeptide amino acid sequence of Fig. 3 IB (SEQ ID NO: 51) with the proviso that any amino acid other than His (denoted as "X 98 ”) is substituted for His at amino acid position 98 (herein referred to as "6G4.2.5LV1 1H98X 98 ").
  • the invention provides an antibody or antibody fragment comprising a light chain comprising the amino acid sequence of amino acids 1-219 of the variant humanized anti-IL-8 6G4.2.5vl 1 light chain polypeptide amino acid sequence of Fig. 31B (SEQ ID NO: 51) with the proviso that any amino acid other than Ser (denoted as " 26") > s substituted for Ser at amino acid position 26 and any amino acid other than Asn (denoted as "X35”) is substituted for Asn at amino acid position 35 (herein referred to as "6G4.2.5LV11S26X 26 /N35X 35 ").
  • the invention provides an antibody or antibody fragment comprising a light chain comprising the amino acid sequence of amino acids 1-219 of the variant humanized anti-IL-8 6G4.2.5vl 1 light chain polypeptide amino acid sequence of Fig. 3 IB (SEQ ID NO: 51) with the proviso that any amino acid other than Asn (denoted as "X35”) is substituted for Asn at amino acid position 35 and any amino acid other than His (denoted as "X ”) is substituted for His at amino acid position 98 (herein referred to as "6G4.2.5LV11N35X 35 /H98X 98 ").
  • the invention provides an antibody or antibody fragment comprising a light chain comprising the amino acid sequence of amino acids 1-219 of the variant humanized anti-IL-8
  • 6G4.2.5vl 1 light chain polypeptide amino acid sequence of Fig. 3 IB (SEQ ID NO: 51) with the proviso that any amino acid other than Ser (denoted as "X26") ' s substituted for Ser at amino acid position 26 and any amino acid other than His (denoted as "X 8 ") is substituted for His at amino acid position 98 (herein referred to as "6G4.2.5LV1 1S26X 26 /H98X 98 ").
  • the invention also encompasses an antibody or antibody fragment comprising a light chain comprising the amino acid sequence of amino acids 1- 19 ofthe variant humanized anti-IL-8 6G4.2.5vl 1 light chain polypeptide amino acid sequence of Fig.
  • the invention provides an antibody or antibody fragment comprising a light chain comprising the amino acid sequence of amino acids 1-219 ofthe variant humanized anti-IL-8 6G4.2.5vl 1 light chain polypeptide amino acid sequence (SEQ ID NO: 56) of Fig. 36 (herein referred to as "6G4.2.5LV1 1N35A").
  • an antibody or antibody fragment comprising a light chain comprising the amino acid sequence of amino acids 1-219 of the variant humanized anti-IL-8 6G4.2.5vl l light chain polypeptide amino acid sequence (SEQ ID NO: 62) of Fig. 45 (herein referred to as "6G4.2.5LV1 1N35E").
  • the invention provides an antibody or antibody fragment comprising a light chain comprising the amino acid sequence of amino acids 1-219 of the variant humanized anti-IL-8 6G4.2.5vl 1 light chain polypeptide amino acid sequence of Fig. 3 IB (SEQ ID NO: 51) with the proviso that Ala is substituted for Ser at amino acid position 26 (herein referred to as "6G4.2.5LV11S26A").
  • the invention provides an antibody or antibody fragment comprising a light chain comprising the amino acid sequence of amino acids 1-219 of the variant humanized anti-IL-8 6G4.2.5vl 1 light chain polypeptide amino acid sequence of Fig. 3 IB (SEQ ID NO: 51) with the proviso that Ala is substituted for His at amino acid position 98 (herein referred to as "6G4.2.5LV11H98A").
  • the invention provides an antibody or antibody fragment comprising a light chain comprising the amino acid sequence of amino acids 1-219 of the variant humanized anti-IL-8 6G4.2.5vl l light chain polypeptide amino acid sequence of Fig. 31B (SEQ ID NO: 51) with the proviso that
  • Ala is substituted for Ser at amino acid position 26 and Ala is substituted for Asn at amino acid position 35
  • the invention provides an antibody or antibody fragment comprising a light chain comprising the amino acid sequence of amino acids 1-219 of the variant humanized anti-IL-8 6G4.2.5vl 1 light chain polypeptide amino acid sequence of Fig. 31 B (SEQ ID NO: 51) with the proviso that
  • Ala is substituted for Ser at amino acid position 26 and Ala is substituted for His at amino acid position 98
  • the invention also encompasses an antibody or antibody fragment comprising a light chain comprising the amino acid sequence of amino acids 1-219 of the variant humanized anti-IL-8 6G4.2.5vl 1 light chain polypeptide amino acid sequence of Fig. 3 IB (SEQ ID NO: 51) with the proviso that Ala is substituted for Asn at amino acid position 35 and Ala is substituted for His at amino acid position 98 (herein referred to as
  • the invention further encompasses an antibody or antibody fragment comprising a light chain comprising the amino acid sequence of amino acids 1-219 ofthe variant humanized anti-IL-8 6G4.2.5vl 1 light chain polypeptide amino acid sequence of Fig. 31 B (SEQ ID NO: 51) with the proviso that Ala is substituted for Ser at amino acid position 26, Ala is substituted for Asn at amino acid position 35, and Ala is substituted for His at amino acid position 98 (herein referred to as "6G4.2.5LV1 1S26A N35A/H98A").
  • the invention provides a single chain antibody fragment comprising a variant light chain selected from the group consisting of 6G4.2.5LV1 ⁇ N35X 35 , 6G4.2.5LV1 1S26X 26 , 6G4.2.5LV1 1H98X 98 , 6G4.2.5LV11S26X 26 / N35X 35 , 6G4.2.5LV1 1N35X 35 / H98X 98 , 6G4.2.5LV1 1S26X 26 /H98X 98 , and 6G4.2.5LV11 S26X 26 / N35X 35 /H98X 98 , with or without any additional amino acid sequence.
  • 6G4.2.5LV1 1S26X 26 / N35X 35 /H98X 98 is collectively referred to herein as the "group of 6G4.2.5LV11X variants", and that individual members of this group are generically referred to herein as a "6G4.2.5LV11X variant.”
  • the invention provides a single chain antibody fragment comprising a 6G4.2.5LV1 IX variant without any associated heavy chain amino acid sequence, i.e. a single chain species that makes up one half of a Fab fragment.
  • the invention provides a
  • 6G4.2.5LV11N35X 35 variant without any associated heavy chain amino acid sequence encompasses a single chain antibody fragment comprising a variant light chain selected from the group consisting of 6G4.2.5LV1 1N35A, 6G4.2.5LV1 1S26A, 6G4.2.5LV11H98A, 6G4.2.5LV11S26A/ N35A, 6G4.2.5LV1 1N35A/ H98A, 6G4.2.5LV1 1S26A/H98A, and 6G4.2.5LV11S26A N35A/H98A, with or without any additional amino acid sequence.
  • the group consisting of 6G4.2.5LV11N35A, 6G4.2.5LV1 1S26A, 6G4.2.5LV1 1H98A, 6G4.2.5LV1 1S26A/ N35A, 6G4.2.5LV1 1N35A/ H98A, 6G4.2.5LV1 1S26A/H98A, and 6G4.2.5LV1 1S26A/ N35A/H98A is collectively referred to herein as the "group of 6G4.2.5LV1 1A variants", and that individual members of this group are generically referred to herein as a "6G4.2.5LV1 1A variant.”
  • the invention provides a single chain antibody fragment comprising a 6G4.2.5LV1 1 A variant without any associated heavy chain amino acid sequence, i.e.
  • the invention provides the 6G4.2.5LV11N35A without any associated heavy chain amino acid sequence.
  • an antibody or antibody fragment comprising a light chain comprising a
  • the invention provides an antibody or antibody fragment comprising a 6G4.2.5LV1 1N35X 35 variant and further comprising the 6G4.2.5HV11.
  • the invention provides an antibody or antibody fragment comprising the 6G4.2.5LV1 1N35A and further comprising the 6G4.2.5HV1 1.
  • the invention provides an antibody or antibody fragment comprising the 6G4.2.5LV1 1N35E and further comprising the 6G4.2.5HV11.
  • the invention provides a single chain antibody fragment wherein a 6G4.2.5LV11X variant and the 6G4.2.5HV11 are contained in a single chain polypeptide species.
  • the single chain antibody fragment comprises a 6G4.2.5LV1 1X variant joined to the 6G4.2.5HV1 1 by means of a flexible peptide linker sequence, wherein the heavy chain and light chain domains can associate in a "dimeric" structure analogous to that formed in a two-chain Fab species.
  • the single chain antibody fragment is a species comprising a 6G4.2.5LV1 IX variant joined to the 6G4.2.5HV1 1 by a linker that is too short to permit intramolecular pairing of complementary domains, i.e. a single chain polypeptide monomer that forms a diabody upon dimerization with another monomer.
  • the invention provides a single chain antibody fragment wherein a
  • the 6G4.2.5LV11N35X 35 variant and the 6G4.2.5HV1 1 are contained in a single chain polypeptide species.
  • the single chain antibody fragment comprises a 6G4.2.5LV1 1N35X 3 5 variant joined to the 6G4.2.5HV1 1 by means of a flexible peptide linker sequence, wherein the heavy chain and light chain domains can associate in a "dimeric" structure analogous to that formed in a two-chain Fab species.
  • the single chain antibody fragment is a species comprising a 6G4.2.5LV1 1N35X 35 variant joined to the 6G4.2.5HV11 by a linker that is too short to permit intramolecular pairing of complementary domains, i.e. a single chain polypeptide monomer that forms a diabody upon dimerization with another monomer.
  • the invention provides a single chain antibody fragment wherein the
  • the 6G4.2.5LV11N35A and the 6G4.2.5HV11 are contained in a single chain polypeptide species.
  • the single chain antibody fragment comprises the 6G4.2.5LV1 1N35A joined to the 6G4.2.5HV11 by means of a flexible peptide linker sequence, wherein the heavy chain and light chain domains can associate in a "dimeric" structure analogous to that formed in a two-chain Fab species.
  • the single chain antibody fragment is a species comprising the 6G4.2.5LV1 1N35A joined to the 6G4.2.5HV11 by a linker that is too short to permit intramolecular pairing of complementary domains, i.e. a single chain polypeptide monomer that forms a diabody upon dimerization with another monomer.
  • the invention provides a single chain antibody fragment wherein the 6G4.2.5LV11N35E and the 6G4.2.5HV1 1 are contained in a single chain polypeptide species.
  • the single chain antibody fragment comprises the 6G4.2.5LV1 1N35E joined to the 6G4.2.5HV11 by means of a flexible peptide linker sequence, wherein the heavy chain and light chain domains can associate in a "dimeric" structure analogous to that formed in a two-chain Fab species.
  • the single chain antibody fragment is a species comprising the 6G4.2.5LV1 1N35E joined to the 6G4.2.5HV11 by a linker that is too short to permit intramolecular pairing of complementary domains, i.e. a single chain polypeptide monomer that forms a diabody upon dimerization with another monomer.
  • the invention provides an antibody fragment comprising a plurality of polypeptide chains, wherein one polypeptide chain comprises a 6G4.2.5LV1 1X variant and a second polypeptide chain comprises the 6G4.2.5HV1 1 and the two polypeptide chains are covalently linked by one or more interchain disulfide bonds.
  • the invention provides an antibody fragment comprising a plurality of polypeptide chains, wherein one polypeptide chain comprises a 6G4.2.5LV1 1N35X 35 variant and a second polypeptide chain comprises the 6G4.2.5HV11 and the two polypeptide chains are covalently linked by one or more- interchain disulfide bonds.
  • any of the foregoing two-chain antibody fragments is selected from the group consisting of Fab, Fab', Fab'-SH, and F(ab') 2 .
  • the two-chain antibody fragment is a F(ab') 2 wherein one polypeptide chain comprises the 6G4.2.5LV1 1N35A and the second polypeptide chain comprises the 6G4.2.5HV1 1.
  • the antibody fragment is a Fab, Fab', Fab'-SH, or F(ab') 2 wherein one polypeptide chain comprises the 6G4.2.5LV1 1N35E and the second polypeptide chain comprises the 6G4.2.5HV1 1.
  • the antibody fragment is the 6G4V1 1N35A F(ab') GCN4 leucine zipper species described in the Examples below.
  • the antibody fragment is the 6G4V11N35E F(ab') 2 GCN4 leucine zipper species described in the Examples below.
  • the antibody fragment is the 6G4V11N35E Fab described in the Examples below.
  • the invention also provides an antibody or antibody fragment comprising a light chain containing a 6G4.2.5LV1 IX variant and optionally further comprising a heavy chain containing the 6G4.2.5HV1 1, wherein the light chain, and optionally the heavy chain, is (are) fused to an additional moiety, such as additional immunoglobulin constant domain sequence.
  • Constant domain sequence can be added to the heavy chain and/or light chain sequence(s) to form species with full or partial length heavy and/or light chain(s). It will be appreciated that constant regions of any isotype can be used for this pu ⁇ ose, including IgG, IgM, IgA, IgD, and IgE constant regions, and that such constant regions can be obtained from any human or animal species.
  • the constant domain sequence is human in origin. Suitable human constant domain sequences can be obtained from Kabat et al.
  • the invention additionally provides an antibody or antibody fragment comprising a light chain containing a 6G4.2.5LV1 1N35X 35 variant and optionally further comprising a heavy chain containing the
  • Constant domain sequence can be added to the heavy chain and/or light chain sequence(s) to form species with full or partial length heavy and/or light chain(s).
  • constant regions of any isotype can be used for this pu ⁇ ose, including IgG, IgM, IgA, IgD, and IgE constant regions, and that such constant regions can be obtained from any human or animal species.
  • the constant domain sequence is human in origin. Suitable human constant domain sequences can be obtained from Kabat et al.
  • the invention further provides an antibody or antibody fragment comprising a light chain containing the 6G4.2.5LV11N35A and optionally further comprising a heavy chain containing the 6G4.2.5HV1 1, wherein the light chain, and optionally the heavy chain, is (are) fused to an additional moiety, such as additional immunoglobulin constant domain sequence.
  • Constant domain sequence can be added to the heavy chain and/or light chain sequence(s) to form species with full or partial length heavy and or light chain(s). It will be appreciated that constant regions of any isotype can be used for this pu ⁇ ose, including IgG, IgM, IgA, IgD, and IgE constant regions, and that such constant regions can be obtained from any human or animal species.
  • the constant dc i lain sequence is human in origin. Suitable human constant domain sequences can be obtained from Kabat et al.
  • the invention further provides an antibody or antibody fragment comprising a light chain containing the 6G4.2.5LV11N35E and optionally further comprising a heavy chain containing the 6G4.2.5HV1 1, wherein the light chain, and optionally the heavy chain, is (are) fused to an additional moiety, such as additional immunoglobulin constant domain sequence.
  • Constant domain sequence can be added to the heavy chain and/or light chain sequence(s) to form species with full or partial length heavy and/or light chain(s).
  • constant regions of any isotype can be used for this pu ⁇ ose, including IgG, IgM, IgA, IgD, and IgE constant regions, and that such constant regions can be obtained from any human or animal species.
  • the constant domain sequence is human in origin. Suitable human constant domain sequences can be obtained from Kabat et al.
  • the antibody or antibody fragment comprises a light chain containing a 6G4.2.5LV1 IX variant, and further comprises the 6G4.2.5HV11 in a heavy chain that is fused to or contains a leucine zipper sequence.
  • the leucine zipper can increase the affinity or production efficiency of the antibody or antibody fragment of interest. Suitable leucine zipper sequences include the jun and fos leucine zippers taught by Kostelney et al, J. Immunol.. 148: 1547-1553 (1992) and the GCN4 leucine zipper described in the Examples below.
  • the antibody or antibody fragment comprises a light chain containing the 6G4.2.5LV1 1N35A, and further comprises a heavy chain containing the 6G4.2.5HV11 fused to the GCN4 leucine zipper.
  • the antibody or antibody fragment comprises a light chain containing the 6G4.2.5LV11N35E, and further comprises a heavy chain containing the 6G4.2.5HV11 fused to the GCN4 leucine zipper.
  • the invention provides humanized antibodies and antibody fragments comprising the CDRs of a 6G4 2 5HV CDR variant
  • the use of a 6G4 2 5HV CDRs variant in the humanized antibodies and antibody fragments of the invention confer the advantages of higher affinity for human IL-8 and/or improved recombinant manufacturing economy
  • a heavy chain variable domain comprising the CDRs of a 6G4 2 5HV CDRs variant can be humanized in conjunction with a light chain comprising the CDRs of 6G4 2 5LV or the CDRs of a 6G4 2 5LV CDRs variant, essentially as described in Section (H)(2)(A) above
  • the invention provides a humanized antibody or antibody fragment comprising a 6G4 2 5HV CDRs variant selected from the group consisting of 6G4 2 5HV/H1S31Z 31 , 6G4 2 5HV/H2S54Z 54 , and 6G4 2 5HV/H1S31Z 31 /H2S54Z 54
  • the invention provides a humanized antibody or antibody fragment comprising a 6G4 2 5HV CDRs variant selected from the group consisting of 6G4 2 5HV/H1S31A, 6G4 2 5HV H2S54A, and 6G4 2 5HV/H1 S31A/H2S
  • the invention additionally provides a humanized antibody or antibody fragment that comprises a heavy chain variable domain comprising the hu6G4 2 5HV/vHl-3Z, and further comprises a light chain variable domain con- ⁇ rising the hu6G4 2 5LV or hu6G4 2 5LV/vL 1-3X
  • the invention further encompasses a single chain humanized antibody fragment comprising the hu6G4 2 5HV/vHl-3Z, with or without any additional amino acid sequence
  • the invention provides a single chain antibody fragment comprising the hu6G4 2 5HV/vHl-3Z without any associated heavy chain variable domain ammo acid sequence, l e a single chain species that makes up one half of an Fv fragment
  • the invention provides a single chain humanized antibody fragment wherein the hu6G4 2 5HV/vHl-3Z and the hu6G4 2 5LV or hu6G4 2 5LV/vLl-3X are contained in a single chain polypeptide species
  • the single chain antibody fragment is a scFv species comprising the hu6G4 2 5HV/vHl-3Z joined to the hu6G4 2 5LV or hu6G4 2 5LV/vLl-3X by means of a flexible peptide linker sequence, wherein the heavy chain and light chain variable domains can associate m a "dimeric" structure analogous to that formed in a two-chain Fv species
  • the single chain antibody fragment is a species comprising the hu6G4 2 5HV/vHl-3Z joined to the hu6G4 2 5LV or hu6G4 2 5LV/vLl-3X by a linker that
  • the invention provides a humanized antibody fragment comprising a plurality of polypeptide chains, wherein one polypeptide chain comprises the hu6G42 5HV/vHl-3Z and a second polypeptide chain comprises the hu6G4 2 5LV or hu6G4 2 5LV/VL1-3X and the two polypeptide chains are covalently linked by one or more interchain disulfide bonds
  • the foregoing two-chain antibody fragment is selected from the group consisting of Fab, Fab', Fab'-SH, Fv, and F(ab') 2 .
  • the invention also provides a humanized antibody or antibody fragment comprising a heavy chain variable domain containing the hu6G4.2.5HV/vHl-3Z and optionally further comprising a light chain variable domain containing the hu6G4.2.5LV or hu6G4.2.5LV/vLl-3X, wherein the heavy chain variable domain, and optionally the light chain variable domain, is (are) fused to an additional moiety, such as an immunoglobulin constant domain.
  • Constant domain sequence can be added to the heavy chain and/or light chain sequence(s) to form species with full or partial length heavy and/or light chain(s).
  • constant regions of any isotype can be used for this pu ⁇ ose, including IgG, IgM, IgA, IgD, and IgE constant regions, and that such constant regions can be obtained from any human or animal species.
  • the constant domain sequence is human in origin. Suitable human constant domain sequences can be obtained from Kabat et al.
  • the humanized antibody or antibody fragment comprises the hu6G4.2.5HV/vHl-3Z in a heavy chain that is fused to or contains a leucine zipper sequence.
  • the leucine zipper can increase the affinity or production efficiency of the antibody or antibody fragment of interest.
  • Suitable leucine zipper sequences include the jun and fos leucine zippers taught by Kostelney et al, 1 Immunol., 148: 1547-1553 (1992) and the GCN4 leucine zipper described in the Examples below.
  • the invention provides a humanized antibody or antibody fragment comprising a heavy chain comprising the amino acid sequence of amino acids 1-230 of the 6G4.2.5HV1 1 polypeptide amino acid sequence of F ⁇ s. 37A-37B (SEQ ID NO: 60) with the proviso that Ala is substituted for Ser at amino acid position 31 (hereinafter referred to as "6G4.2.5HV1 1S31 A").
  • the invention provides a humanized antibody or antibody fragment comprising a heavy chain comprising the amino acid sequence of amino acids 1-230 of the 6G4.2.5HV1 1 polypeptide amino acid sequence of Figs. 37A-37B (SEQ ID NO: 60) with the proviso that Ala is substituted for Ser at amino acid position 54 (hereinafter referred to as "6G4.2.5HV1 1S54A").
  • the invention provides a humanized antibody or antibody fragment comprising a heavy chain comprising the amino acid sequence of amino acids 1-230 of the 6G4.2.5HV11 polypeptide amino acid sequence of Figs. 37A-37B (SEQ ID NO: 60) with the proviso that Ala is substituted for Ser at amino acid position 31 and Ala is substituted for Ser at amino acid position 54 (hereinafter referred to as "6G4.2.5HV1 1S31A/S54A").
  • the invention encompasses a single chain humanized antibody fragment comprising a variant heavy chain selected from the group consisting of 6G4.2.5HV11S31A, 6G4.2.5HV11S54A, and 6G4.2.5HV11S31A/ S54A, with or without any additional amino acid sequence.
  • the group consisting of 6G4.2.5HV11S31A, 6G4.2.5HV1 1S54A, and 6G4.2.5HV11 S31A/ S54A is collectively referred to herein as the "group of 6G4.2.5HV11A variants", and that individual members of this group are generically referred to herein as a "6G4.2.5HV11A variant.”
  • the invention provides a single chain humanized antibody fragment comprising a 6G4.2.5HV11A variant without any associated light chain amino acid sequence, i.e. a single chain species that makes up one half of a Fab fragment.
  • a humanized antibody or antibody fragment comprising a heavy chain comprising a 6G4.2.5HV11A variant, and further comprising a light chain comprising a 6G4.2.5LV11A variant or a 6G4.2.5LV11X variant.
  • the humanized antibody or antibody fragment comprises any combination of light and heavy chains listed in Table 1 above.
  • the invention provides a humanized antibody or antibody fragment comprising a 6G4.2.5HV1 1A variant and further comprising the 6G4.2.5LV11N35X 35 .
  • the invention provides a humanized antibody or antibody fragment comprising a 6G4.2.5HV1 1A variant and further comprising the 6G4.2.5LV1 1N35A.
  • the invention provides a single chain humanized antibody fragment wherein a 6G4.2.5HV1 1 A variant and the 6G4.2.5LV1 1 are contained in a single chain polypeptide species.
  • the invention provides a single chain humanized antibody fragment wherein any pair of light and heavy chains listed in Table 1 above is contained in a single chain polypeptide species.
  • the invention provides a single chain humanized antibody fragment where ' i a 6G4.2.5HV11A variant and a 6G4.2.5LV11X variant are contained in a single chain polypeptide species.
  • the invention provides a single chain humanized antibody fragment wherein a 6G4.2.5HV11A variant and a 6G4.2.5LV1 1N35X 35 variant are contained in a single chain polypeptide species.
  • the invention provides a single chain humanized antibody fragment wherein a
  • 6G4.2.5.IV1 1 A variant and the 6G4.2.5LV1 1N35A variant are contained in a single chain polyj ⁇ tide species.
  • the single chain humanized antibody fragment comprises a f-G4.2.5HVl 1 A variant joined to a 6G4.2.5LV1 IX variant, 6G4.2.5LV1 1N35X 35 variai ., 6G4.2.5LV1 1N35A variant, or 6G4.2.5LV1 1 by means of a flexible peptide linker sequence, wherein the heavy chain and light chain domains can associate in a "dimeric" structure analogous to that formed in a two-chain Fab species.
  • the single chain humanized antibody fragment is a species comprising a 6G4.2.5HV1 1 A variant joined to a 6G4.2.5LV11X variant, 6G4.2.5LV11N35X 35 variant, 6G4.2.5LV11N35A variant, or 6G4.2.5LV11 by a linker that is too short to permit intramolecular pairing of complementary domains, i.e. a single chain polypeptide monomer that forms a diabody upon dimerization with another monomer.
  • the single chain humanized antibody fragment comprises any pair of light and heavy chains listed in Table 1 above joined by means of a flexible peptide linker sequence, wherein the heavy chain and light chain domains can associate in a "dimeric" structure analogous to that formed in a two-chain Fab species.
  • the single chain humanized antibody fragment comprises any pair of light and heavy chains listed in Table 1 above joined by a linker that is too short to permit intramolecular pairing of complementary domains, i.e. a single chain polypeptide monomer that forms a diabody upon dimerization with another monomer.
  • the invention provides a humanized antibody fragment comprising a plurality of polypeptide chains, wherein one polypeptide chain comprises a 6G4.2.5HV11A variant and a second polypeptide chain comprises a 6G4.2.5LV11X variant, 6G4.2.5LV1 1N35X 35 variant, 6G4.2.5LV11N35A variant, or 6G4.2.5LV11, and the two polypeptide chains are covalently linked by one or more interchain disulfide bonds.
  • the foregoing two-chain antibody fragment is selected from the group consisting of Fab, Fab', Fab'-SH, and F(ab') 2 .
  • the invention provides a two-chain humanized antibody fragment comprising any pair of heavy and light chains listed in Table 1 above, wherein each chain is contained on a separate molecule.
  • the two-chain antibody fragment comprising any pair of heavy and light chains listed in Table 1 above is selected from the group consisting of Fab, Fab', Fab'-SH, and F(ab') 2 .
  • the two-chain humanized antibody fragment is a F(ab') 2 comprising any pair of heavy and light chains listed in Table 1 above.
  • the two-chain humanized antibody fragment is a F(ab') 2 wherein one polypeptide chain comprises a 6G4.2.5HV1 1A variant and the second polypeptide chain comprises the 6G4.2.5LV11N35A.
  • the invention also provides a humanized antibody or antibody fragment comprising a heavy chain containing a 6G4.2.5HV11A variant and optionally further comprising a lb ⁇ t chain containing a
  • Constant domain sequence can be added to the heavy chain and/or light chain sequence(s) to form species with full or partial length heavy and/or light chain(s). It will be appreciated that constant regions of any isotype can be used for this pu ⁇ ose, including IgG, IgM, IgA, IgD, and IgE constant regions, and that such constant regions can be obtained from any human or animal species.
  • the constant domain sequence is human in origin. Suitable human constant domain sequences can be obtained from Kabat et al. (supra).
  • the humanized antibody or antibody fragment comprises a 6G4.2.5HV11A variant in a heavy chain that is fused to or contains a leucine zipper sequence.
  • the leucine zipper can increase the affinity or production efficiency of the antibody or antibody fragment of interest.
  • Suitable leucine zipper sequences include the jun and fos leucine zippers taught by Kostelney et al, J. Immunol., 148: 1547-1553 (1992) and the GCN4 leucine zipper described in the Examples below.
  • Bispecific antibodies are monoclonal, preferably human or humanized, antibodies that have binding specificities for at least two different antigens.
  • one of the binding specificities is for IL-8, the other one is for any other antigen.
  • bispecific antibodies specifically binding a IL-8 and neurotrophic factor, or two different types of IL-8 polypeptides are within the scope of the present invention.
  • Methods for making bispecific antibodies are known in the art. Traditionally, the recombinant production of bispecific antibodies is based on the co-expression of two immunoglobulin heavy chain-light chain pairs, where the two heavy chains have different specificities (Milstein and Cuello, Nature 305:537 (1983)).
  • antibody-variable domains with the desired binding specificities are fused to immunoglobulin constant-domain sequences.
  • the fusion preferably is with an immunoglobulin heavy-chain constant domain, comprising at least part of the hinge, CH2, and CH3 regions. It is preferred to have the first heavy-chain constant region (CHI), containing the site necessary for light-chain binding, present in at least one ofthe fusions.
  • DNAs encoding the immunoglobulin heavy chain fusions and, if desired, the immunoglobulin light chain are inserted into separate expression vectors, and are co-transfected into a suitable host organism.
  • the bispecific antibodies are composed of a hybrid immunoglobulin heavy chain with a first binding specificity in one arm, and a hybrid immunoglobulin heavy chain-light chain pair (providing a second binding specificity) in the other arm.
  • This asymmetric structure facilitates the separation of the desired bispecific compound from unwanted immunoglobulin chain combinations, as the presence of an immunoglobulin light chain in only one half cf the bispecific molecule provides for a facile way of separation.
  • bispecific antibodies see, for example, Suresh et al. Methods in Enzvmology 121 :210 (1986).
  • the interface between a pair of antibody molecules can be engineered to maximize the percentage of heterodimers which are recovered from recombinant cell culture.
  • the preferred interface comprises at least a part of the C H 3 domain of an antibody constant domain.
  • one or more small amino acid side chains from the interface of the first antibody molecule are replaced with larger side chains (e.g. tyrosine or tryptophan).
  • Compensatory "cavities" of identical or similar size to the large side chain(s) are created on the interface ofthe second antibody molecule by replacing large amino acid side chains with smaller ones (e.g. alanine or threonine). This provides a mechanism for increasing the yield of the heterodimer over other unwanted end-products such as homodimers.
  • Bispecific antibodies include cross-linked or "heteroconjugate" antibodies.
  • one of the antibodies in the heteroconjugate can be coupled to avidin, the other to biotin.
  • Such antibodies have, for example, been proposed to target immune system cells to unwanted cells (US Patent No. 4,676,980), and for treatment of HIV infection (WO 91/00360, WO 92/00373, and EP 03089).
  • Heteroconjugate antibodies may be made using any convenient cross-linking methods. Suitable cross-linking agents are well known in the art, and are disclosed in US Patent No. 4,676,980, along with a number of cross-linking techniques.
  • bispecific antibodies can be prepared using chemical linkage.
  • Brennan et al, Science, 229: 81 (1985) describe a procedure wherein intact antibodies are proteolytically cleaved to generate
  • F(ab') 2 fragments These fragments are reduced in the presence of the dithiol complexing agent sodium arsenite to stabilize vicinal dithiols and prevent intermolecular disulfide formation.
  • the Fab' fragments generated are then converted to thionitrobenzoate (TNB) derivatives.
  • TAB thionitrobenzoate
  • One of the Fab'-TNB derivatives is then reconverted to the Fab'-thiol by reduction with mercaptoethylamine and is mixed with an equimolar amount of the other Fab'-TNB derivative to form the bispecific antibody.
  • the bispecific antibodies produced can be used as agents for the selective immobilization of enzymes.
  • T cells as well as trigger the lytic activity of human cytotoxic lymphocytes against human breast tumor targets.
  • bispecific antibodies have been produced using leucine zippers.
  • the leucine zipper peptides from the Fos and Jun proteins were linked to the Fab' portions of two different antibodies by gene fusion.
  • the antibody homodimers were reduced at the hinge region to form monomers and then re-oxidized to form the antibody heterodimers.
  • This method can also be utilized for the production of antibc )v homodimers.
  • Antibodies with more than two valencies are contemplated.
  • trispecific antibodies can be prepared. Tutt et al. J. Immunol. 147: 60 (1991).
  • the antibodies and antibody fragments of the invention can be produced using any convenient antibody manufacturing process known in the art. Typically, the antibody or antibody fragment is made using recombinant expression systems. A multiple polypeptide chain antibody or antibody fragment species can be made in a single host cell expression system wherein the host cell produces each chain of the antibody or antibody fragment and assembles the polypeptide chains into a multimeric structure to form the antibody or antibody fragment in vivo, followed by recovery of the antibody or antibody fragment from the host cell.
  • suitable recombinant expression systems for the production of complete antibody or antibody fragment are described in Lucas et al, Nucleic Acids Res., 24: 1774-1779 (1996).
  • the separate polypeptide chains of the desired antibody or antibody fragment can be made in separate expression host cells, separately recovered from the respective host cells, and then mixed in vitro under conditions permitting the formation ofthe multi-subunit antibody or antibody fragment of interest.
  • U.S. Pat. No. 4,816,567 to Cabilly et al. and Carter et al, Bio/Technology, K): 163-167 (1992) provide methods for recombinant production of antibody heavy and light chains in separate expression hosts followed by assembly of antibody from separate heavy and light chains in vitro.
  • DNA encoding the desired antibody or antibody fragment can be prepared by a variety of methods known in the art. These methods include, but are not limited to, chemical synthesis by any of the methods described in Engels et al, Agnew. Chem. Int. Ed. Engl..
  • fusion proteins wherein the gene(s) encoding the antibody or antibody fragment is associated, in the vector, with a gene encoding another protein or a fragment of another protein. This results in the antibody or antibody fragment being produced by the host cell as a fusion with another protein.
  • the "other" protein is often a protein or peptide which can be secreted by the cell, making it possible to isolate and purify the desired protein from the culture medium and eliminating the necessity of destroying the host cells which arises when the desired protein remains inside the cell.
  • the fusion protein can be expressed intracellularly. It is advantageous to use fusion proteins that are highly expressed.
  • Fusion proteins can be cleaved using chemicals, such as cyanogen bromide, which cleaves at a methionine, or hydroxylamine, which cleaves between an Asn and Gly.
  • chemicals such as cyanogen bromide, which cleaves at a methionine, or hydroxylamine, which cleaves between an Asn and Gly.
  • the nucleotide base pairs encoding these amino acids may be inserted just prior to the 5' end of the antibody or antibody fragment gene(s).
  • proteolytic cleavage of fusion proteins which has been recently reviewed (Carter, P. (1990) in Protein Purification: From Molecular Mechanisms to Large-Scale Processes, Ladisch, M. R., Willson, R. C, Painton, C. C, and Builder, S. E., eds., American Chemical Society Symposium Series No. 427, Ch 13, 181-193).
  • Proteases such Factor Xa, thrombin, subtilisin and mutants thereof, have been successfully used to cleave fusion proteins.
  • a peptide linker that is amenable to cleavage by the protease used is inserted between the "other" protein (e.g., the Z domain of protein A) and the protein of interest, such as humanized anti-IL-8 antibody or antibody fragment.
  • the nucleotide base pairs encoding the linker are inserted between the genes or gene fragmc..s coding for the other proteins. Proteolytic cleavage of the partially purified fusion protein containing the correct linker can then be carried out on either the native fusion protein, or the reduced or denatured fusiov. protein.
  • the DNA encoding the antibody or antibody fragment is inserted into a replicable vector for further cloning (amplification of the DNA) or for expression.
  • a replicable vector for further cloning (amplification of the DNA) or for expression.
  • Many vectors are available, and selection of the appropriate vector will depend on (1) whether it is to be used for DNA amplification or for DNA expression, (2) the size of the DNA to be inserted into the vector, and (3) the host cell to be transformed with the vector.
  • Each vector contains various components depending on its function (amplification of DNA or expression of DNA) and the host cell for which it is compatible.
  • the vector components generally include, but are not limited to, one or more of the following: a signal sequence, an origin of replication, one or more marker genes, an enhancer element, a promoter, and a transcription termination sequence.
  • Signal Sequence Component generally include, but are not limited to, one or more of the following: a signal sequence, an origin of replication, one or more marker genes, an enhancer element, a promoter,
  • a signal sequence may be a component of the vector, or it may be a part of the antibody or antibody fragment DNA that is inserted into the vector.
  • a heterologous signal sequence selected and fused to the antibody or antibody fragment DNA such that the signal sequence in the corresponding fusion protein is recognized, transported and processed (i.e., cleaved by a signal peptidase) in the host cell's protein secretion system.
  • the signal sequence is selected, for example, from the group of the alkaline phosphatase, penicillinase, lpp, or heat-stable enterotoxin II leaders.
  • the STII signal sequence is used as described in the Examples below.
  • yeast secretion the native signal sequence may be substituted by, e.g., the yeast invertase leader, a factor leader
  • mammalian signal sequences as well as viral secretory leaders for example, the he ⁇ es simplex gD signal, are available.
  • Both expression and cloning vectors contain a nucleic acid sequence that enables the vector to replicate in one or more selected host cells.
  • this sequence is one that enables the vector to replicate independently ofthe host chromosomal DNA, and includes origins of replication or autonomously replicating sequences.
  • origins of replication or autonomously replicating sequences are well known for a variety of bacteria, yeast, and viruses.
  • the origin of replication from the plasmid pBR322 is suitable for most Gram-negative bacteria, the 2 ⁇ plasmid origin is suitable for yeast, and various viral origins (SV40, polyoma, adenovirus, VSV or BPV) are useful for cloning vectors in mammalian cells.
  • the origin of replication component is not needed for mammalian expression vectors (the SV40 origin may typically be used only because it contains the early promoter).
  • Most expression vectors are "shuttle" vectors, i.e. they are capable of replication in at least one class of organisms but can be transfected into another organism for expression.
  • a vector is cloned in E. coli and then the same vector is transfected into yeast or mammalian cells for expression even though it is not capable of replicating independently ofthe host cell chromosome.
  • DNA may also be amplified by insertion into the host genome. This is readily accomplished using Bacillus species as hosts, for example, by including in the vector a DNA sequence that is homologous to a sequence found in Bacillus genomic DNA. Transfection of Bacillus with this vector results in homologous recombination with the genome and insertion ofthe antibody or antibody fragment DNA.
  • Selection genes should contain a selection gene, also termed a selectable marker. This gene encodes a protein necessary for the survival or growth of transformed host cells grown in a selective culture medium. Host cells not transformed with the vector containing the selection gene will not survive in the culture medium.
  • Typical selection genes encode proteins that (a) confer resistance to antibiotics or other toxins, e.g. ampicillin, neomycin, methotrexate. or tetracycline, (b) complement auxotrophic deficiencies, or (c) supply critical nutrients not available from complex media, e.g. the gene encoding D-alanine racemase for Bacilli.
  • a selection scheme utilizes a drug to arrest growth of a host cell. Those cells that are successfully transformed with a heterologous gene express a protein conferring drug resistance and thus survive the selection regimen. Examples of such dominant selection use the drugs neomycin (Southern et al, J. Molec. Appl. Genet., 327 (1982)), mycophenolic acid (Mulligan et al, Science, 209: 1422 (1980)) or hygromycin (Sugden et al, Mol. Cell. Biol., 5: 410-413 (1985)).
  • the three examples given above employ bacterial genes under eukaryotic control to convey resistance to the appropriate drug (G418 or neomycin (geneticin), xgpt (mycophenolic acid), and hygromycin, respectively.)
  • suitable selectable markers for mammalian cells are those that enable the identification of cells competent to take up the antibody or antibody fragment nucleic acid, such as dihydrofolate reductase (DHFR) or thymidine kinase.
  • DHFR dihydrofolate reductase
  • the mammalian cell transformants are placed under selection pressure which only the transformants are uniquely adapted to survive by virtue of having taken up the marker. Selection pressure is imposed by culturing the transformants under conditions in which the concentration of selection agent in the medium is successively changed, thereby leading to amplification of both the selection gene and the DNA that encodes the antibody or antibody fragment.
  • Amplification is the process by which genes in greater demand for the production of a protein critical for growth are reiterated in tandem within the chromosomes of successive generations of recombinant cells. Increased quantities of the antibody or antibody fragment are synthesized from the amplified DNA.
  • cells transformed with the DHFR selection gene are first identified by culturing all of the transformants in a culture medium that contains methotrexate (Mtx), a competitive antagonist of DHFR.
  • Mtx methotrexate
  • An appropriate host cell when wild-type DHFR is employed is the Chinese hamster ovary (CHO) cell line deficient in DHFR activity, prepared and propagated as described by Urlaub and Chasin, Proc. Natl. Acad. Sci. USA, 77: 4216 (1980).
  • the transformed cells are then exposed to increased levels of methotrexate. This leads to the synthesis of multiple copies of the DHFR gene, and, concomitantly, multiple copies of other DNA comprising the expression vectors, such as the DNA encoding the antibody or antibody fragment.
  • This amplification technique can be used with any otherwise suitable host, e.g., ATCC No. CCL61 CHO-K1, notwithstanding the presence of endogenous DHFR if, for example, a mutant DHFR gene that is highly resistant to Mtx is employed (EP 117,060).
  • host cells transformed or co-transformed with DNA sequences encoding the antibody or antibody fragment, wild-type DHFR protein, and another selectable marker such as aminoglycoside 3' phosphotransferase (APH) can be selected by cell growth in medium containing a selection agent for the selectable marker such as an aminoglycosidic antibiotic, e.g., kanamycin, neomycin, or G418. See U.S. Pat. No. 4,965,199.
  • APH aminoglycoside 3' phosphotransferase
  • a suitable selection gene for use in yeast is the trp ⁇ gene present in the yeast plasmid YRp7. Stinchcomb et al, Nature, 282: 39 (1979); Kingsman et al, Gene, 7: 141 (1979); or Tschemper et al, Gene. .10: 157 (1980).
  • the trp ⁇ gene provides a selection marker for a mutant strain of yeast lacking the ability to grow in tryptophan, for example, ATCC No. 44076 or PEP4-1. Jones, Genetics, 85: 12 (1977). The presence of the trp_l lesion in the yeast host cell genome then provides an effective environment for detecting transformation by growth in the absence of tryptophan.
  • Promoter Component Expression vectors usually contain a promoter that is recognized by the host organism and is operably linked to the antibody or antibody fragment nucleic acid. Promoters are untranslated sequences located upstream (5') to the start codon of a structural gene (generally within about 100 to 1000 bp) that control the transcription and translation of a particular nucleic acid sequence, such as the antibody or antibody fragment encoding sequence, to which they are operably linked. Such promoters typically fall into two classes, inducible and constitutive.
  • Inducible promoters are promoters that initiate increased levels of transcription from DNA under their control in response to some change in culture conditions, e.g. the presence or absence of a nutrient or a change in temperature. At this time a large number of promoters recognized by a variety of potential host cells are well known.
  • Promoters suitable for use with prokaryotic hosts include the ⁇ -lactamase and lactose promoter systems (Chang et al, Nature, 275: 615 (1978); and Goeddel et al, Nature, 281 : 544 (1979)), alkaline phosphatase, a tryptophan (t ⁇ ) promoter system (Goeddel, Nucleic Acids Res.. 8: 4057 (1980) and EP 36,776) and hybrid promoters such as the tac promoter (deBoer et al, Proc. Natl. Acad. Sci. USA, 80: 21-25 (1983)).
  • other known bacterial promoters are suitable.
  • Suitable promoting sequences for use with yeast hosts include the promoters for 3-phosphoglycerate kinase (Hitzeman et al, J. Biol. Chem., 255: 2073 (1980)) or other glycolytic enzymes (Hess et al, J. Adv. Enzyme Reg..
  • yeast promoters which are inducible promoters having the additional advantage of transcription controlled by growth conditions, are the promoter regions for alcohol dehydrogenase 2, isocytochrome C, acid phosphatase, degradative enzymes associated with nitrogen metabolism, metallothionein, glyceraldehyde-3- phosphate dehydrogenase, and enzymes responsible for maltose and galactose utilization.
  • Suitable vectors and promoters for use in yeast expression are further described in Hitzeman et al, EP 73,657A.
  • Yeast enhancers also are advantageously used with yeast promoters. Promoter sequences are known for eukaryotes.
  • Virtually all eukaryotic genes have an AT-rich region located approximately 25 to 30 bases upstream from the site where transcription is initiated. Another sequence found 70 to 80 bases upstream from the start of transcription of many genes is a CXCAAT region where X may be any nucleotide. At the 3' end of most eukaryotic genes is an AATAAA sequence that may be the signal for addition ofthe poly A tail to the 3' end of the coding sequence. All of these sequences are suitably inserted into mammalian expression vectors.
  • Vector driven transcription of antibody or antibody fragment encoding DNA in mammalian host cells can be controlled by promoters obtained from the genomes of viruses such as polyoma virus, fowlpox virus (UK 2,211,504 published 5 July 1989), adenovirus (such as Adenovirus 2), bovine papilloma virus, avian sarcoma virus, cytomegalovirus, a retrovirus, hepatitis-B virus and most preferably Simian Virus 40 (SV40), from heterologous mammalian promoters, e.g. the actin promoter or an immunoglobulin promoter, and from heat-shock promoters, provided such promoters are compatible with the host cell systems.
  • viruses such as polyoma virus, fowlpox virus (UK 2,211,504 published 5 July 1989), adenovirus (such as Adenovirus 2), bovine papilloma virus, avian sarcoma virus, cytomegalovirus,
  • the early and late promoters of the SV40 virus are conveniently obtained as an SV40 restriction fragment that also contains the SV40 viral origin of replication. Fiers et al, Nature, 273: 113 (1978); Mulligan and Berg, Science, 209: 1422-1427 (1980); Pavlakis et al, Proc. Natl. Acad. Sci. USA, 78: 7398-7402 (1981).
  • the immediate early promoter of the human cytomegalovirus is conveniently obtained as a HindHI E restriction fragment. Greenaway et al, Gene, 18: 355-360 (1982).
  • a system for expressing DNA in mammalian hosts using the bovine papilloma virus as a vector is disclosed in U.S. 4,419,446.
  • Enhancers are cis- acting elements of DNA, usually about from 10-300 bp, that act on a promoter to increase its transcription. Enhancers are relatively orientation and position independent having been found 5' (Laimins et al, Proc. Natl. Acad. Sci. USA. 78: 993 (1981)) and 3' (Lusky et al, Mol.
  • Examples include the SV40 enhancer on the late side of the replication origin (bp 100- 270), the cytomegalovirus early promoter enhancer, the polyoma enhancer on the late side of the replication origin, and adenovirus enhancers. See also Yaniv, Nature, 297: 17-18 (1982) on enhancing elements for activation of eukaryotic promoters.
  • the enhancer may be spliced into the vector at a position 5' or 3' to the antibody or antibody fragment DNA, but is preferably located at a site 5' from the promoter, (vi) Transcription Termination Component
  • Expression vectors used in eukaryotic host cells can also contain sequences necessary for the termination of transcription and for stabilizing the mRNA. Such sequences are commonly available from the 5' and, occasionally 3' untranslated regions of eukaryotic or viral DNAs or cDNAs. These regions contain nucleotide segments transcribed as polyadenylated fragments in the untranslated portion of the mRNA encoding the antibody or antibody fragment. The 3' untranslated regions also include transcription termination sites. Suitable vectors containing one or more of the above listed components and the desired coding and control sequences are constructed by standard ligation techniques. Isolated plasmids or DNA fragments are cleaved, tailored, and religated in the form desired to generate the plasmids required.
  • the ligation mixtures are used to transform E. coli K12 strain 294 (ATCC 31 ,446) and successful transformants selected by ampicillin or tetracycline resistance where appropriate. Plasmids from the transformants are prepared, analyzed by restriction endonuclease digestion, and/or sequenced by the method of Messing et al, Nucleic Acids Res., 9:
  • transient expression involves the use of an expression vector that is able to replicate efficiently in a host cell, such that the host cell accumulates many copies ofthe expression vector and, in turn, synthesizes high levels of a desired polypeptide encoded by the expression vector.
  • Suitable host cells for cloning or expressing the vectors herein are the prokaryote, yeast, or higher eukaryote cells described above.
  • Suitable prokaryotes include eubacteria, such as Gram-negative or Gram-positive organisms, for example, E. coli, Bacilli such as B. subtilis, Pseudomonas species such as P. aeruginosa, Salmonella typhimurium, or Serratia marcescens.
  • E. coli cloning host is E. coli 294 (ATCC 31,446), although other strains such as E. coli B, E. coli 1776 (ATCC 31,537), and E. coli W3110 (ATCC 27,325) are suitable.
  • the host cell should secrete minimal amounts of proteolytic enzymes.
  • the E. coli strain 49D6 is used as the expression host as described in the Examples below. Review articles describing the recombinant production of antibodies in bacterial host cells include Skerra et al, Curr. Opinion in Immunol., 5: 256 (1993) and Pluckthun, Immunol. Revs.. 130: 151 (1992).
  • eukaryotic microbes such as filamentous fungi or yeast are suitable hosts for vectors containing antibody or antibody fragment DNA.
  • Saccharomyces cerevisiae, or common baker's yeast is the most commonly used among lower eukaryotic host microorganisms.
  • S. pombe Beach and Nurse, Nature. 290: 140 (1981)
  • Kluyveromyces lactis Kluyveromyces lactis
  • Host cells derived from multicellular organisms can also be used in the recombinant production of antibody or antibody fragment. Such host cells are capable of complex processing and glycosylation activities. In principle, any higher eukaryotic cell culture is workable, whether from vertebrate or invertebrate culture. Examples of invertebrate cells include plant and insect cells.
  • baculoviral strains and variants and corresponding permissive insect host cells from hosts such as Spodoptera frugiperda (cate ⁇ illar), Aedes aegypti (mosquito), Aedes albopictus (mosquito), Drosophila melanogaster (fruitfly), and Bombyx mori host cells have been identified. See, e.g., Luckow et al, Bio/Technology. 6: 47-55 (1988); Miller et al, in Genetic Engineering. Setlow, J.K. et at, 8: 277-279 (Plenum Publishing, 1986), and Maeda et al, Nature, 315: 592- 594 (1985).
  • viruses are publicly available, e.g., the L-l variant of Autographa californica NP V and the Bm-5 strain of Bombyx mori NPV,.and such viruses may be used as the virur herein according to the present invention, particularly for transfection of Spodoptera frugiperda cells.
  • Plant cell cultures of cotton, corn, potato, soybean, petunia, tomato, and tobacco can be utilized as hosts.
  • plant cells are transfected by incubation with certain strains of the bacterium Agrobacterium tumefaciens, which has been previously manipulated to contain the antibody or antibody fragment DNA.
  • A. tumefaciens the DNA encoding antibody or antib -y fragment is transferred to the plant cell host such that it is transfected, and will, under appropriate conditions, express the antibody or antibody fragment DNA.
  • regulatory and signal sequences compatible with plant cells are available, such as the nopaline synthase promoter and polyadenylation signal sequences. Depicker et al, J. Mol.
  • DNA segments isolated from the upstream region of the T-DNA 780 gene are capable of activating or increasing transcription levels of plant-expressible genes in recombinant DNA-containing plant tissue. See EP 321,196 published 21 June 1989.
  • Vertebrate cell culture is preferred for the recombinant production of full length antibodies.
  • tissue culture The propagation of vertebrate cells in culture (tissue culture) has become a routine procedure in recent years (Tissue Culture. Academic Press, Kruse and Patterson, editors (1973)).
  • useful mammalian host cell lines are monkey kidney CV1 line transformed by SV40 (COS-7, ATCC CRL 1651); human embryonic kidney line (293 or 293 cells subcloned for growth in suspension culture, Graham et al, J. Gen Virol., 36: 59 (1977)); baby hamster kidney cells (BHK, ATCC CCL 10); Chinese hamster ovary cells/-DHFR (CHO, Urlaub and Chasin, Proc. Natl. Acad. Sci.
  • mice sertoli cells TM4, Mather, Biol. Reprod.. 23: 243-251 (1980)); monkey kidney cells (CV1 ATCC CCL 70); African green monkey kidney cells (VERO- 76, ATCC CRL-1587); human cervical carcinoma cells (HELA, ATCC CCL 2); canine kidney cells (MDCK, ATCC CCL 34); buffalo rat liver cells (BRL 3A, ATCC CRL 1442); human lung cells (W138, ATCC CCL 75); human liver cells (Hep G2, HB 8065); mouse mammary tumor (MMT 060562, ATCC CCL51); TRI cells (Mather et al, Annals N.Y. Acad.
  • MRC 5 cells are human embryonic kidney 293 and Chinese hamster ovary cells.
  • Myeloma cells that do not otherwise produce immunoglobulin protein are also useful host cells for the recombinant production of full length antibodies.
  • Host cells are transfected and preferably transformed with the above-described expression or cloning vectors of this invention and cultured in conventional nutrient media modified as appropriate for inducing promoters, selecting transformants, or amplifying the genes encoding the desired sequences.
  • Transfection refers to the taking up of an expression vector by a host cell whether or not any coding sequences are in fact expressed. Numerous methods of transfection are known to the ordinarily skilled artisan, for example, CaP0 4 precipitation and electroporation. Successful transfection is generally recognized when any indication ofthe operation of this vector occurs within the host cell.
  • Transformation means introducing DNA into an organism so that the DNA is replicable, either as an extrachromosomal element or by chromosomal integrant. Depending on the host cell used, transformation is done using standard techniques appropriate to such cells.
  • the calcium treatment employing calcium chloride, as described in section 1.82 of Sambrook et al, supra, is generally used for prokaryotes or other cells that contain substantial cell-wall barriers. Infection with Agrobacterium tumefaciens is used for transformation of certain plant cells, as described by Shaw et al, Gene. 23: 315 (1983) and WO 89/05859 pub.ished 29 June 1989.
  • Prokaryotic cells used to produce the antibody or antibody fragment are cultured in suitable media as described generally in Sambrook et al, supra.
  • the mammalian host cells used to produce the antibody or antibody fragment can be cultured in a variety of media.
  • Commercially available media such as Ham's F10 (Sigma), Minimal Essential Medium ((MEM), Sigma), RPMI- 1640 (Sigma), and Dulbecco's Modified Eagle's Medium ((DMEM), Sigma) are suitable for culturing the host cells.
  • Pat. Re. 30,985; or U.S. 5,122,469 may be used as culture media for the host cells. Any of these media may be supplemented as necessary with hormones and/or other growth factors (such as insulin, transferrin, or epidermal growth factor), salts (such as sodium chloride, calcium, magnesium, and phosphate), buffers (such as HEPES), nucleosides (such as adenosine and thymidine), antibiotics (such as Gentamycin drug), trace elements (defined as inorganic compounds usually present at final concentrations in the micromolar range), and glucose or an equivalent energy source.
  • growth factors such as insulin, transferrin, or epidermal growth factor
  • salts such as sodium chloride, calcium, magnesium, and phosphate
  • buffers such as HEPES
  • nucleosides such as adenosine and thymidine
  • antibiotics such as Gentamycin drug
  • trace elements defined as inorganic compounds usually present at final concentrations in the micromolar range
  • the culture conditions such as temperature, pH, and the like, are those previously used with the host cell selected for expression, and will be apparent to the ordinarily skilled artisan.
  • the host cells referred to in this disclosure encompass cells in in vitro culture as well as cells that are within a host animal.
  • Gene amplification and/or expression may be measured in a sample directly, for example, by conventional Southern blotting, northern blotting to quantitate the transcription of mRNA (Thomas, Proc. Natl. Acad. Sci. USA. 77: 5201-5205 (1980)), dot blotting (DNA analysis), or in situ hybridization, using an appropriately labeled probe, based on the sequences provided herein.
  • Various labels may be employed, most
  • radioisotopes particularly P.
  • biotin-modified nucleotides for introduction into a polynucleotide.
  • the biotin then serves as the site for binding to avidin or antibodies, which may be labeled with a wide variety of labels, such as radionuclides, fluorescers, enzymes, or the like.
  • antibodies may be employed that can recognize specific duplexes, including DNA duplexes, RNA duplexes, and DNA-RNA hybrid duplexes or DNA-protein duplexes.
  • the antibodies in turn may be labeled and the assay may be carried out where the duplex is bound to a surface
  • Gene expression may be measured by immunological methods, such as immunohistochemical staining of tissue sections and assay of cell culture or body fluids, to quantitate directly the expression of gene product.
  • immunohistochemical staining techniques a cell sample is prepared, typically by dehydration and fixation, followed by reaction with labeled antibodies specific for the gene product, where the labels are usually visually detectable, such as enzymatic labels, fluorescent labels, luminescent labels, and the like.
  • a particularly sensitive staining technique suitable for use in the present invention is described by Hsu et al, Am. J. Clin. Path.. 75: 734-738 (1980).
  • the antibody or antibody fragment is recovered from the culture medium.
  • the antibody can be produced intracellularly, or produced in the periplasmic space of a bacterial host cell. If the antibody is produced intracellularly, as a first step, the host cells are lysed, and the resulting particulate debris is removed, for example, by centrifiigation or ultrafiltration. Carter et al, Bio/Technology 10: 163-167 (1992) describe a procedure for isolating antibodies which are secreted to the periplasmic space of E. coli.
  • cell paste is thawed in the presence of sodium acetate (pH 3.5), EDTA, and phenylmethylsulfonylfluoride (PMSF) over about 30 min.
  • PMSF phenylmethylsulfonylfluoride
  • Cell debris can be removed by centrifiigation.
  • supematants from such expression systems are generally first concentrated using a commercially available protein concentration filter, for example, an Amicon or Millipore Pellicon ultrafiltration unit.
  • a protease inhibitor such as PMSF may be included in any of the foregoing steps to inhibit proteolysis and antibiotics may be included to prevent the growth of adventitious contaminants.
  • the antibody composition prepared from the cells can be purified using, for example, hydroxylapatite chromatography, gel electrophoresis, dialysis, and affinity chromatography, with affinity chromatography being the preferred purification technique.
  • affinity chromatography is the preferred purification technique.
  • the suitability of protein A as an affinity ligand depends on the species and isotype of any immunoglobulin Fc domain that is present in the antibody.
  • Protein A can be used to purify antibodies that are based on human ⁇ l, ⁇ 2, or ⁇ 4 heavy chains (Lindmark et al, J. Immunol. Meth. 62: 1-13 (1983)).
  • Protein G is recommended for all mouse isotypes and for human ⁇ 3 (Guss et al, EMBO J. 5: 15671575 (1986)).
  • the matrix to which the affinity ligand is attached is most often agarose, but other matrices are available.
  • Mechanically stable matrices such as controlled pore glass or poly(styrenedivinyl)benzene allow for faster flow rates and shorter processing times than can be achieved with agarose.
  • the antibody comprises a C H 3 domain
  • the Bakerbond ABXTMresin J. T. Baker, Phillipsburg,
  • the mixture comprising the antibody of interest and contaminants may be subjected to low pH hydrophobic interaction chromatograph. using an elution buffer at a pH between about 2.5-4.5, preferably performed at low salt concentrations (e.g. from about 0-0.25M salt).
  • F(ab') 2 fragments can be isolated directly from recombinant host cell culture. Other techniques for the production of antibody fragments will be apparent to the skilled practitioner. 5. Uses of Anti-IL-8 Antibodies
  • the antibodies or antibody fragments of the invention typically will be labeled with a detectable moiety.
  • the detectable moiety can be any one which is capable of producing, either directly or indirectly, a detectable signal.
  • the detectable moiety can be a radioisotope, such as H, C, P, S, or I; a fluorescent or chemiluminescent compound, such as fluorescein isothiocyanate, rhodamine, or luciferin; radioactive isotopic labels, such as, e.g., I, P, C, or H; or an enzyme, such as alkaline phosphatase, beta-galactosidase, or horseradish peroxidase.
  • a radioisotope such as H, C, P, S, or I
  • a fluorescent or chemiluminescent compound such as fluorescein isothiocyanate, rhodamine, or luciferin
  • radioactive isotopic labels such as, e.g., I, P, C, or H
  • an enzyme such as alkaline phosphatase, beta-galactosidase, or horseradish peroxidase.
  • any method known in the art for separately conjugating the antibody or antibody fragment to the detectable moiety can be employed, including those methods described by Hunter et al, Nature 144:945 (1962); David et al, Biochemistry 13:1014 (1974); Pain et al. J. Immunol. Meth. 40:219 (1981); and Nygren, J. Histochem. and Cvtochem. 30:407 (1982).
  • the antibodies and antibody fragments of the present invention can be employed in any known assay method, such as competitive binding assays, direct and indirect sandwich assays, and immunoprecipitation assays.
  • competitive binding assays rely on the ability of a labeled standard (which can be a IL-8 or an immunologically reactive portion thereof) to compete with the test sample analyte (IL-8) for binding with a limited amount of antibody or antibody fragment.
  • IL-8 analyte
  • the amount of IL-8 in the test sample is inversely proportional to the amount of standard that becomes bound to the antibodies.
  • the antibodies or antibody fragments generally are insolubilized before or after the competition, so that the standard and analyte that are bound to the antibodies can conveniently be separated from the standard and analyte which remain unbound.
  • Sandwich assays involve the use of two antibodies, each capable of binding to a different antigenic portion, or epitope, of the protein (IL-8) to be detected.
  • the test sample analyte is bound by a first antibody which is immobilized on a solid support, and thereaft'.r ⁇ a second antibody binds to the analyte, thus forming an insoluble three-part complex (U.S. Patent No. 4,376,110).
  • the second antibody can itself be labeled with a detectable moiety (direct sandwich assays) or can be measured using an anti- immunoglobulin antibody that is labeled with a detectable moiety (indirect sandwich assay).
  • sandwich assay is an ELISA assay, in which case the detectable moiety is an enzyme (e.g., horseradish peroxidase).
  • IL-8 antibodies and antibody fragments also are useful for the affinity purification of IL-8 from recombinant cell culture or natural sources.
  • these antibodies can be fixed to a solid support by techniques well known in the art so as to purify IL-8 from a source such as culture supernatant or tissue.
  • the humanized anti-IL-8 antibodies and antibody fragments ofthe invention are useful in the treatment of inflammatory disorders, including inflammations of the lung, such as adult respiratory distress syndrome (ARDS) and any stage of acute lung injury in the pathogenesis of ARDS described in Bernard et al., Am. J. Respir. Crit. Care Med..
  • bacterial pneumonia a chronic respiratory disease 2019
  • hypovolemic shock ischemic reperfusion disorders
  • myocardial ischemic conditions such as myocardial infarction, reperfusion after cardiac surgery, cardiac arrest, and constriction after percutaneous transluminal coronary angioplasty
  • inflammatory bowel disorders such is ulcerative colitis
  • autoimmune diseases such as rheumatoid arthritis.
  • the humanized anti-IL-8 antibodies and antibody fragments ofthe invention are useful in the treatment of asthmatic diseases, such as allergic asthma.
  • Therapeutic formulations ofthe humanized anti-IL-8 antibodies and antibody fragments are prepared for storage by mixing the antibody or antibody fragment having the desired degree of purity with optional physiologically acceptable carriers, excipients, or stabilizers (Remington's Pharmaceutical Sciences, supra), in the form of lyophilized cake or aqueous solutions.
  • Acceptable carriers, excipients or stabilizers are nontoxic to recipients at the dosages and concentrations employed, and include buffers such as phosphate, citrate, and other organic acids; antioxidants including ascorbic acid; low molecular weight (less than about 10 residues) polypeptides; proteins, such as serum albumin, gelatin, or immunoglobulins; hydrophilic polymers such as polyvinylpyrrolidone; amino acids such as glycine, glutamine, asparagine, arginine or lysine; monosaccharides, disaccharides, and other carbohydrates including glucose, mannose, or dextrins; chelating agents such as EDTA; sugar alcohols such as mannitol or sorbitol; salt-forming counterions such as sodium; and/or nonionic surfactants such as Tween, Pluronics or polyethylene glycol (PEG).
  • buffers such as phosphate, citrate, and other organic acids
  • antioxidants including ascorbic acid
  • the humanized anti-IL-8 mAb or antibody fragment to be used for in vivo administration must be sterile. This is readily accomplished by filtration through sterile filtration membranes, prior to or following lyophilization and reconstitution.
  • the humanized anti-IL-8 mAb or antibody fragment ordinarily will be stored in lyophilized form or in solution.
  • Therapeutic humanized anti-IL-8 mAb or antibody fragment compositions generally are placed into a container having a sterile access port, for example, an intravenous solution bag or vial having a stopper pierceable by a hypodermic injection needle.
  • humanized anti-IL-8 mAb or antibody fragment administration is in accord with known methods, e.g., inhalation, injection or infusion by intravenous, intraperitoneal, intracerebral, intramuscular, intraocular, intraarterial, or intralesional routes, by enema or suppositjry, or by sustained release systems as noted below.
  • the antibody is given systemically or at a site of inflammation.
  • the invention provides for the treatment of asthmatic diseases by administration of humanized anti-IL-8 mAb or antibody fragment to the respiratory tract.
  • the invention contemplates formulations comprising humanized anti-IL-8 mAb or antibody fragment for use in a wide variety of devices that are designed for the delivery of pharmaceutical compositions ai. therapeutic formulations to the respiratory tract.
  • humanized anti-IL-8 mAb or antibody fragment is administered in aerosolized or inhaled form.
  • the humanized anti-IL-8 mAb or antibody fragi'ient, combined with a dispersing agent, or dispersant, can be administered in an aerosol formulation as a dry powder or in a solution or suspension with a diluent.
  • Suitable dispersing agents are well known in the art, and include but are not limited to surfactants and the like.
  • Surfactants are generally used in the art to reduce surface induced aggregation of protein caused by atomization of the solution forming the liquid aerosol.
  • examples of such surfactants include polyoxyethylene fatty acid esters and alcohols, and polyexyethylene sorbitan fatty acid esters. Amounts of surfactants used will vary, being generally within the range of about 0.001 to 4% by weight of the formulation. In a specific aspect, the surfactant is polyoxyethylene sorbitan monooleate or sorbitan trioleate.
  • Liquid aerosol formulations contain the humanized anti-IL-8 mAb or antibody fragment and a dispersing agent in a physiologically acceptable diluent.
  • the dry powder formulations ofthe invention consist of a finely divided solid form of the humanized anti-IL-8 mAb or antibody fragment and a dispersing agent, and optionally a bulking agent, such as lactose, sorbitol, sucrose, or mannotil, and the like, to facilitate dispersal of the powder.
  • the formulations must be aerosolized. It must be broken down into liquid or solid particles in order to ensure that the aerosolized dose actually reaches the bronchii and/or alveoli, as desired.
  • the mass median dynamic diameter will be 5 micrometers ( ⁇ m) or less to ensure that the drug particles reach the lung bronchii or alveoli (Wearly, L.L., 1991, Crit. Rev. in Ther. Drug Carrier Systems, 8:333).
  • any form of aerosolization known in the art including but not limited to nebulization, atomization or pump aerosolization of a liquid formulation, and aerosolization of a dry powder formulation, can be used in the practice ofthe invention.
  • a delivery device that is uniquely designed for administration of solid formulations is envisioned.
  • the aerosolization of a liquid or a dry powder formulation will require a propellent.
  • the propellent can be any propellent generally used in the art.
  • Examples of useful propellants include cholorofluorocarbons, hydrofluorocarbons, hydrochlorofluorocarbons, and hydrocarbons, including trifluoromethane, dichlorofluoromethane, dichlorotetrafluoroethanol, and 1,1,1,2-tetrafluoroethane, and combinations thereof.
  • the device for aerosolization is a metered dose inhaler.
  • a metered dose inhaler provides a specific dosage when administered, rather than a variable dose depending on administration.
  • Such a metered dose inhaler can be used with either a liquid or a dry powder aerosol formulation.
  • Sustained release systems can be used in the practice of :he methods of the invention.
  • Suitable examples of sustained-release preparations include semipermeable polymer matrices in the form of shaped articles, e.g. films, or microcapsules.
  • Sustained release ma.rices include polyesters, hydrogels, polylactides (U.S. 3,773,919, EP 58,481), copolymers of L-glutamic acid and gamma ethyl-L-glutamate (Sidman et al, Biopolvmers 22:547 (1983)), poly (2-hydroxyethyl-methacrylate) (Langer et al, J. Biomed. Mater. Res.
  • Sustained-release humanized anti-IL-8 antibody or antibody fragment compositions also include liposomally entrapped antibody or antibody fragment.
  • Liposomes containing an antibody or antibody fragment are prepared by methods known per se: DE 3,218,121; Epstein et al, Proc. Natl. Acad. Sci. U.S.A. 82:3688 (1985); Hwang et al, Proc. Natl. Acad. Sci. U.S.A.
  • the liposomes are of the small (about 200-800 Angstroms) unilamelar type in which the lipid content is greater than about 30 mole percent cholesterol, the selected proportion being adjusted for the most efficacious antibody or antibody fragment therapy.
  • an "effective amount" of the humanized anti-IL-8 antibody or antibody fragment to be employed therapeutically will depend, for example, upon the therapeutic objectives, the route of administration, and the condition of the patient. Accordingly, it will be necessary for the therapist to titer the dosage and modify the route of administration as required to obtain the optimal therapeutic effect. Typically, the clinician will administer the humanized anti-IL-8 antibody or antibody fragment until a dosage is reached that achieves the desired effect. The progress of this therapy is easily monitored by conventional assays. In the treatment and prevention of an inflammatory disorder or asthmatic disorder with a humanized anti-IL-8 antibody or antibody fragment of the invention, the antibody composition will be formulated, dosed, and administered in a fashion consistent with good medical practice.
  • Factors for consideration in this context include the particular disorder being treated, the particular mammal being treated, the clinical condition of the individual patient, the cause ofthe disorder, the site of delivery of the antibody, the particular type of antibody, the method of administration, the scheduling of administration, and other factors known to medical practitioners.
  • the "therapeutically effective amount" of antibody to be administered will be governed by such considerations, and is the minimum amount necessary to prevent, ameliorate, or treat the inflammatory disorder, including treating acute or chronic respiratory diseases and reducing inflammatory responses. Such amount is preferably below the amount that is toxic to the host or renders the host significantly more susceptible to infections.
  • the initial pharmaceutically effective amount of the antibody or antibody fragment administered parenterally per dose will be in the range of about 0.1 to 50 mg/kg of patient body weight per day. with the typical initial range of antibody used being 0.3 to 20 mg/kg/day, more preferaoly 0.3 to 15 mg/kg/day. In one embodiment, using systemic administration, the initial pharmaceutically effective amount will be in the range of about 2 to 5 mg/kg/day.
  • the initial pharmaceutically effective amount will be in the range of about 1 microgram ( ⁇ g)/kg/day to 100 mg/kg/day.
  • the invention provides for both prophylactic and th ⁇ .apeutic treatment of inflammatory disorders. Without intending to limit the methods of the invention to a particular mechanism of action or a particular disease intervention strategy, it is noted that in some indications it is beneficial to treat the disease in question prior to or early on in the stage of the underlying disease that involves neutrophil activation, recruitment and infiltration at sites of inflammation. Accordingly, it may be advantageous to utilize the humanized anti-IL-8 mAb or antibody fragment in a prophylactic treatment regimen for an inflammatory disease indication in order to attenuate or eliminate a pathogenic neutrophil response that may or will arise during the course of the disease.
  • a prophylactic course of treatment in order to ameliorate or prevent the deterioration of lung function and the pathogenesis of associated disease sequelae (which may greatly increase patient morbidity and mortality) prior to the onset of such conditions.
  • Certain biological parameters such as IL-8 levels in bronchial alveolar lavage (BAL) fluid and ferritin levels in serum, can be used for prognosis of acute lung injury and ARDS in patients who are predisposed to such disease progression, i.e.
  • acute lung injury and ARDS at-risk patients presenting BAL fluid IL-8 concentrations of at or above 0.2 ng/ml are selected for prophylactic treatment according to the methods ofthe invention.
  • Any suitable method for assay of IL-8 in patient BAL fluid may be employed, such as the method described in Donnelly et al., Lancet, 341 : 643-647 (1993).
  • acute lung injury/ARDS at-risk female and male patients presenting ferritin serum concentrations of at or above 270 ng/ml and 680 ng/ml, respectively, are selected for prophylactic treatment according to the methods ofthe invention.
  • Any suitable method for assay of ferritin in patient serum may be employed, such as the method described in U.S. Pat. No. 5,679,532 for "Serum Ferritin as a Predictor ofthe Acute Respiratory Distress Syndrome" to Repine issued on October 21, 1997.
  • the humanized anti-IL-8 mAb or antibody fragment is administered to the patient prior to or concomitant with recanalization therapy, including pharmaceutical recanalization therapies such as the administration of tissue plasminogen activators, streptokinase, or other thrombolytic drugs with or without anti-clotting agents such as platelet-fibrin binding antagonists (e.g.
  • the humanized anti-IL-8 mAb or antibody fragment of the invention can be employed in the methods of treating acute myocardial infarction with anti-IL-8 antibody described in WO 97/40215 published October 30, 1997.
  • the invention provides for both prophylactic and therapeutic treatment of asthma with humanized anti-IL-8 mAb and antibody fragment.
  • prophylactic treatment for allergic asthma with the antibodies or antibody fragments of the invention it is desirable to administer about 0.1 to 10 mg kg of the antibody agent to the patient up to about 24 hours prior to anticipated exposure to allergen or prior to onset of allergic asthma.
  • therapeutic treatment for acute asthma including allergic asthma, it is desirable to treat the asthmatic patient as early as possible following onset of an asthma attack.
  • an episode of acute asthma is treated within 24 hours of the onset of symptoms by administration of about 0.1 to 10 mg/kg of an anti-IL-8 antibody agent.
  • the methods ofthe invention can be used to ameliorate symptoms at any point in the pathogenesis of asthmatic disease. Additionally, the methods ofthe invention can be used to alleviate symptoms of chronic asthmatic conditions.
  • the antibody or antibody fragment need not be, but is optionally formulated with one or more agents currently used to prevent or treat the inflammatory disorder or asthmatic disease in question.
  • the antibody in rheumatoid arthritis, the antibody can be given in conjunction with a glucocorticosteroid.
  • the invention contemplates the coadministration of antibody or antibody fragment and one or more additional agents useful in treating asthma, such as bronchodilators, antihistamines, epinephrine, and the like.
  • the effective amount of such other agents depends on the amount of antibody or antibody fragment present in the formulation, the type of disorder or treatment, and other factors discussed above. These are generally used in the same dosages and with administration routes as used hereinbefore or about from 1 to 99% ofthe heretofore employed dosages.
  • mice were immunized in each hind footpad or intraperitoneally with 10 ⁇ g of recombinant human IL-8 (produced as a fusion of (ser-IL-8) 72 with ubiquitin (Hebert et al. J. Immunology 145:3033-3040
  • IL-8 is available commercially from PeproTech, Inc., Rocky Hill, NJ) resuspended in MPL/TDM (Ribi Immunochem. Research Inc., Hamilton, MT) and boosted twice with the same amount of IL-8.
  • "IL-8" is intended to mean (ser-IL-8) 72 unless otherwise specified.
  • a final boost of 10 ⁇ g of IL-8 was given 3 days before the fusion.
  • Spleen cells or popliteal lymph node cells were fused with mouse myeloma P3X63Ag8U.l (ATCC CRL1597). non-secreting clone of the myeloma P3X63Ag8, using 35% polyethylene glycol as described before.
  • Ten days after the fusion, culture supernatant was screened for the presence of monoclonal antibodies to IL-8 by ELISA.
  • the ELISA was performed as follows. Nunc 96-well immunoplates (Flow Lab, McLean, VA) were coated with 50 ⁇ l/well of 2 ⁇ g/ml IL-8 in phosphate-buffered saline (PBS) overnight at 4°C. The remaining steps were carried out at room temperature. Nonij.ecific binding sites were blocked with 0.5% bovine serum albumin (BSA) for 1 hour (hr).
  • BSA bovine serum albumin
  • the isotypes of the monoclonal antibodies were determined by coating Nunc 96-well immunoplates (Flow Lab, McLean, VA) with IL-8 overnight, blocking with BSA, incubating with culture supematants followed by the addition of predetermined amount of isotype-specific alkaline phosphatase-conjugated goat anti-mouse Ig (Fisher Biotech, Pittsburgh, PA). The level of conjugated antibodies bound to the plate was determined by the addition of r- nitrophenyl phosphate as described above.
  • ELISA was performed. At a ratio of biotinylated mAb to unlabeled mAb of 1 : 100, the binding of biotinylated mAb 5.12.14 was significantly inhibited by its homologous mAb but not by mAb 4.1.3, while the binding of biotinylated mAb 4.1.3 was inhibited by mAb 4.1.3 but not by mAb 5.12.14. Monoclonal antibody 5.2.3 behaved similarly to mAb 4.1.3, while monoclonal antibodies 4.8 and 12.3.9 were similar to mAb 5.12.14. Thus, mAb 4.1.3 and mAb 5.2.3 bind to a different epitope(s) than the epitope recognized by monoclonal antibodies 12.3.9, 4.8 and 5.12.14.
  • Immunodot blot analysis was performed to assess antibody reactivity to IL-8 immobilized on nitrocellulose paper. All seven antibodies recognized IL-8 immobilized on paper, whereas a control mouse IgG antibody did not.
  • 125 4 radioimmune precipitation test (RIP). Briefly, tracer I-IL-8 (4 x 10 cpm) was incubated with various dilutions of the monoclonal anti-IL-8 antibodies in 0.2 ml of PBS containing 0.5% BSA and 0.05% Tween 20 (assay buffer) for 1 hr at room temperature. One hundred microliters of a predetermined concentration of goat anti-mouse Ig antisera (Pel-Freez, Rogers, AR) were added and the mixture was incubated at room temperature for 1 hr. Immune complexes were precipitated by the addition of 0.5 ml of 6% polyethylene glycol (M.W. 8000) kept at 4°C.
  • M.W. 8000 polyethylene glycol
  • Neutrophils were prepared by using Mono-Poly Resolving Medium (M- PRM) (Flow Lab. Inc., McLean, VA). Briefly fresh, heparinized human blood was loaded onto M-PRM at a ratio of blood to medium, 3.5:3.0, and centrifuged at 300 x g for 30 min at room temperature. Neutrophils enriched at the middle layer were collected and washed once in PBS. Such a preparation routinely contained greater than 95% neutrophils according to the Wright's Giemsa staining. The receptor binding assay was done
  • I-IL-8 50 ⁇ l of I-IL-8 (5 ng/ml) was incubated with 50 ⁇ l of unlabeled IL-8 (100 ⁇ g/ml) or monoclonal antibodies in PBS containing 0.1% BSA for 30 min at room temperature. The mixture was then incubated with
  • the I-IL-8 bound was separated from the unbound material by loading mixtures onto 0.4 ml of PBS containing 20% sucrose and 0.1% BSA and by centrifiigation at 300 x g for 15 min. The supernatant was removed by aspiration and the radioactivity associated with the pellet was counted in a gamma counter.
  • Monoclonal antibodies 4.1.3, 5.2.3, 4.8, 5.12.14, and 12.3.9 inhibited greater than 85% of the binding of IL-8 to human neutrophils at a 1 :25 molar ratio of IL-8 to mAb.
  • monoclonal antibodies 9.2.4 and 8.9.1 appeared to enhance the binding of IL-8 to its receptors on human neutrophils. Since a control mouse IgG also enhanced the binding of IL-8 on neutrophils, the enhancement of IL-8 binding to its receptors by mAb 9.2.4 and 8.9.1 appears to be nonspecific.
  • monoclonal antibodies, 4.1.3, 5.1.3, 4.8, 5.12.14, and 12.3.9 are potential neutralizing monoclonal antibodies while monoclonal antibodies 8.9.1 and 9.2.4 are non-neutralizing monoclonal antibodies.
  • the ability of the anti-IL-8 antibodies to block neutrophil chemotaxis induced by IL-8 was tested as follows. Neutrophil chemotaxis induced by IL-8 was determined using a Boyden chamber method (Larsen, et al. Science 243: 1464 (1989)). One hundred ⁇ l of human neutrophils (10 cells/ml) resuspended in RPMI containing 0.1% BSA were placed in the upper chamber and 29 ⁇ l of the IL-8 (20 nM) with or without monoclonal antibodies were placed in the lower chamber. Cells were incubated for 1 hr at 37°C.
  • Neutrophils migrated into the lower chamber were stained with Wright's Giemsa stain and counted under the microscope ( 1 OOx magnification). Approximately 10 different fields per experimental group were examined. Neutralizing monoclonal antibodies 5.12.14 and 4.1.3 blocked almost 70% of the neutrophil chemotactic activity of IL-8 at 1 :10 ratio of IL-8 to mAb.
  • the isoelectric fo ⁇ -sing (IEF) pattern of each mAb was determined by applying purified antibodies on an IEF polyacrylamide gel (pH 3-9, Pharmacia) using the Fast gel system (Pharmacia, Piscataway, NJ).
  • the IEF gel was pretreated with pharmalyte containing 1% Triton XI 00 (Sigma, St. Louis, MO) for 10 min before loading the samples.
  • the IEF pattern was visualized by silver staining according to the instructions from the manufacturer. All of the monoclonal antibodies had different IEF patterns, confirming that they originated from different clones.
  • the pi values for the antibodies are listed in Table 3.
  • IL-8 has greater than 30% sequence homology with certain other members of the platelet factor 4 (PF4) family of inflammatory cytokines such as ⁇ -TG (Van Damme et al, Eur. J. Biochem. 181 :337(1989); Tanaka et al, FEB 236(2):467 (1988)) and PF4 (Deuel et al, Proc. Natl. Acad. Sci. U.S.A.
  • PF4 platelet factor 4
  • mAb 5.12.14 was further studied to determine whether it could block the IL-8 mediated release of elastase by neutrophils. Briefly, human neutrophils were resuspended in Hanks balanced salt solution (Gibco, Grand Island, NY) containing 1.0% BSA, Fraction V (Sigma, St. Louis, MO), 2 mg/ml alpha-D-glucose (Sigma), 4.2 mM sodium bicarbonate (Sigma) and 0.01 M HEPES, pH 7.1 (JRH Bioscience, Lenexa, KS). A stock of cytochalasin B (Sigma) was prepared (5 mg/ml in dimethylsulfoxide (Sigma) and stored at 2-8°C.
  • Cytochalasin B was added to the neutrophil preparation to produce a final concentration of 5 ⁇ g/ml, and incubated for 15 min at 37°C.
  • Human IL-8 was incubated with mAb 5.12.14 (20 ⁇ l), or a negative control antibody, in 1 ml polypropylene tubes (DBM Scientific, San Fernando, CA) for 30 min at 37°C.
  • the final assay concentrations of IL-8 were 50 and 500 nM.
  • the monoclonal antibodies were diluted to produce the following ratios (IL-8:Mab): 1 :50, 1:10, 1 :2, 1 : 1, and 1 :0.25.
  • Cytochalasin B-treated neutrophils were added (100 ⁇ l/tube) and incubated for 2 hours at 25°C.
  • the tubes were centrifuged (210 X g, 2-8°C) for 10 min, and supematants were transferred to 96 well tissue culture plates (30 ⁇ l/well).
  • Elastase substrate stock 10 mM methoxysuccinyl-alanyl-alanyl-propyl-valyl-p-nitroanilide (Calbiochem, La Jolla, CA) in DMSO was prepared and stored at 2-8°C.
  • Elastase substrate solution ( 1.2 mM substrate, 1.2 M NaCl (Mallinckrodt, Paris, Kentucky), 0.12 M HEPES pH 7.2 in distilled water) was added (170 ⁇ l/well) to the supematants and incubated for 0.5 to 2 hours at 37°C (until control O.D. of 1.0 was reached). Absorbance was measured at 405 nm (SLT 340 ATTC plate reader, SLT Lab Instruments, Austria). The results are shown in Figure 1. At a 1 : 1 ratio of IL-8 to mAb 5.12.14, the antibody was able to effectively block the release of elastase from neutrophils.
  • the hybridoma producing antibody 5.12.14 was deposited on February 15, 1993 with the American Type Culture Collection, 12301 Parklawn Drive, Rockville, MD, U.S.A. (ATCC) and assigned ATCC Accession No. HB 11553.
  • ATCC American Type Culture Collection
  • Antibodies against rabbit IL-8 were generated in essentially the same process as anti-human IL-8 antibodies using rabbit IL-8 as immunogen (kindly provided by C. Broaddus; see also Yoshimura et al. ⁇ : Immunol. 146:3483 (1991)). The antibody was characterized as described above for binding to other cytokine_. coated onto ELISA plates; no measurable binding was found to MGSA, fMLP, C5a, b-TG, TNF, PF4, or IL-1.
  • the hybridoma producing antibody 6G4.2.5 was deposited on September 28, 1994, with the American Type Culture Collection, 12301 Parklawn Drive, Rockville, MD, U.S.A. (ATCC) and assigned ATTC Accession No. HB 11722.
  • Recombinant human-murine chimeric Fabs for 5.12.14 and 6G4.2.5 were constructed as described below.
  • a chimeric 6G.4.25 Fab is compared with a chimeric 5.12.14 Fab in detail below.
  • the ability of the two chimeric Fabs, 5.12.14-Fab and 6G4.2.5-Fab, to efficiently bind IL-8 and prevent IL-8 from binding to IL-8 receptors on human neutrophils was determined by performing a competition binding assay which allows the calculation of the IC 5 o - concentration required to achieve 50% inhibition of IL-8 binding.
  • Human neutrophils (5 X 10 ) were incubated for 1 hour at 4°C with 0.5nM I-IL-8 in the presence of various concentrations (0 to 300 nM) of 5.12.14-Fab, 6G4.2.5-Fab, an isotype control (4D5-Fab) or
  • HBSS Hank's balanced salt solution
  • bovine serum albumin 0.1% bovine serum albumin.
  • the neutrophils were labeled by adding calcein AM (Molecular Probe, Eugene, OR) at a final concentration of 2.0 ⁇ M. Following a 30 minute incubation at 37°C, cells were washed twice with HBSS-BSA and resuspended at 5 x 10 6 cells/ml.
  • Chemotaxis experiments were carried out in a Neuro Probe (Cabin John, MD) 96-well chamber, model MBB96. Experimental samples (buffer only control, IL-8 alone or IL-8 + Fabs) were loaded in a Neuro Probe (Cabin John, MD) 96-well chamber, model MBB96. Experimental samples (buffer only control, IL-8 alone or IL-8 + Fabs) were loaded in a Neuro Probe (Cabin John, MD) 96-well chamber, model MBB96. Experimental samples (buffer only control, IL-8 alone or IL-8 + Fabs) were loaded in a
  • Figure 6 demonstrates the inhibition of human IL-8 mediated neutrophil chemotaxis by chimeric 6G4.2.5 and 5.12.14 Fabs.
  • Figure 7 demonstrates the relative abilities of chimeric 6G4.2.5 and 5.12.14 Fabs to inhibit rabbit IL-8 mediated neutrophil chemotaxis.
  • the final neutrophil pellet was suspended at a concentration of 1 x 10 7 cells/ml in assay buffer, which consisted of Hanks Balanced Salt Solution (GIBCO, Grand Island, NY) supplemented with 1.0% BSA (fraction V, Sigma, St. Louis, MO), 2 mg/ml glucose, 4.2 mM sodium bicarbonate, and 0.01 M HEPES, pH 7.2.
  • assay buffer consisted of Hanks Balanced Salt Solution (GIBCO, Grand Island, NY) supplemented with 1.0% BSA (fraction V, Sigma, St. Louis, MO), 2 mg/ml glucose, 4.2 mM sodium bicarbonate, and 0.01 M HEPES, pH 7.2.
  • IL-8 (10 ⁇ l) was mixed with anti-IL-8 Fab, an isotype control Fab, or buffer (20 ⁇ l) in 1 ml polypropylene tubes and incubated in a 37°C water bath for 30 min.
  • IL-8 was used at final concentrations ranging from 0.01 to 1000 nM in dose response studies ( Figure 8) and at a final concentration of 100 nM in the experiments addressing the effects of the Fabs on elastase release ( Figures 9 and 10).
  • Fab concentrations ranged from approximately 20 nM to 300 nM, resulting in Fab:IL-8 molar ratios of 0.2: 1 to 3:1.
  • Cytochalasin B (Sigma) was added to the neutrophil suspension at a concentration of 5 ⁇ g/ml (using a 5mg/ml stock solution made up in DMSO), and the cells were incubated for 15 min in a 37°C water bath. Cytochalasin B-treated neutrophils (100 ⁇ l) were then added to the IL-8/Fab mixtures. After a 3 hr incubation at room temperature, the neutrophils were pelleted by centrifiigation (200 x g for 5 min), and aliquots of the cell-free supematants were transferred to 96 well plates (30 ⁇ l/well).
  • the elastase substrate methoxysuccinyl-alanyl-alanyl-prolyl- valyl-p-nitroanilide (Calbiochem, La Jolla, CA), was prepared as a 10 mM stock solution in DMSO and stored at 4°C.
  • Elastase substrate working solution was prepared just prior to use (1.2 mM elastase substrate, 1.2 M NaCl, 0.12 M HEPES, pH 7.2), and 170 ⁇ l was added to each sample-containing well.
  • the plates were placed in a 37°C tissue culture incubator for 30 min or until an optical density reading for the positive controls reached at least 1.0. Absorbance was measured at 405 nm using an SLT 340 plate reader (SLT Lab Instruments, Austria).
  • Figure 9 demonstrates the ability of the chimeric anti-IL-8 Fabs to inhibit elastase release from human neutrophils stimulated by human IL-8;
  • Figure 10 demonstrates the relative abilities of the chimeric anti-IL-8 Fabs to inhibit elastase release from human neutrophils stimulated by rabbit IL-8.
  • C MOLECULAR CLONING OF THE VARIABLE LIGHT AND HEAVY REGIONS OF THE MURINE 5.12.14 (ANTI-IL-8) MONOCLONAL ANTIBODY
  • Three primers (SEQ ID NOS: 1-6; were designed for each of the light and heavy chains to increase the chances of primer hybridization and efficiency of first strand cDNA synthesis (Figure 13).
  • Amplification of the first strand cDNA to double-stranded (ds) DNA was accomplished using two sets of synthetic DNA oligonucleotide primers: one forward primer (SEQ ID NOS: 7- 9) and one reverse primer (SEQ ID NO: 10) for the light chain variable region amplification ( Figure 14) and one forward primer (SEQ ID NOS: 11-14) and one reverse primer (SEQ ID NOS: 11, 15, 14 and 13) for the heavy chain variable region amplification ( Figure 15).
  • the N-terminal sequence of the first eight amino acids of either the light or heavy chains of 5.12.14 was used to generate a putative murine DNA sequence corresponding to this region. (A total of 29 amino acids was sequenced from the N-terminus of both the light chain and heavy chain variable regions using the Edman degradation protein sequencing technique.) This information was used to design the forward amplification primers which were made degenerate in the third position for some codons to increase the chances of primer hybridization to the natural murine DNA codons and also included the unique restriction site, Mlul, for both the light chain variable region forward primer and the heavy chain variable region forward primer to facilitate ligation to the 3' end of the STII element in the cloning vector.
  • the reverse amplification primers were designed to anneal with the murine DNA sequence corresponding to a portion of the constant region of the light or heavy chains near the variable/constant junction.
  • the light chain variable region reverse primer contained a unique BstBI restriction site and the heavy chain variable region reverse primer contained a unique Apal restriction site for ligation to the 5' end of either the human IgGl constant light or IgGl constant heavy regions in the vectors, pB13.1 (light chain) and pB14 (heavy chain).
  • the polymerase chain reaction using these primer sets yielded DNA fragments of approximately 400 bp.
  • the cDNA encoding the 5.12.14 light chain variable region was cloned into the vector pB 13.1, to form pA51214VL and the 5.12.14 heavy chain variable region was cloned into the vector, pB14, to form pA51214VH.
  • the cDNA inserts were characterized by DNA sequencing and are presented in the DNA sequence (SEQ ID NO: 16) and amino acid sequence (SEQ ID NO: 17) of Figure 16 (murine light chain variable region) and in the DNA sequence (SEQ ID NO: 18) and amino acid (SEQ ID NO: 19) of Figure 17 (murine heavy chain variable region).
  • FIG. 18 shows the amplification primers used to make these modifications.
  • the forward primer, VL. front (SEQ ID NO: 20), was designed to match the last five amino acids ofthe STII signal sequence, including the Mlul cloning site, and the first 4 amino acids of the 5.12.1 murine light chain variable sequence. The sequence was altered from the original »DNA in the third position of the first two codons Dl (T to C) and 12 (C to T) to create a unique EcoRV cloning site which v. as used for later constructions.
  • VL.rear The reverse primer, VL.rear (SEQ ID NO: 21 ), was ⁇ esigned to match the first three amino acids ofthe human IgGl constant light sequence and the last seven amino acids ofthe 5.12.14 light chain variable sequence which included a unique BstBI cloning site.
  • the nucleotide sequence encoding several amino acids were altered: L106 (TTG to CTT), K107 (AAA to CGA) resulting in a conservative amino acid substitution to arginine, and R108 (CGG to AGA).
  • the PCR product encoding the modified 5.12.14 light chain variable sequence was then subcloned into pB13.1 in a two- part ligation.
  • the MluI-BstBI digested 5.12.14 PCR product encoding the light chain variable region was ligated into MluI-BstBI digested vector to form the plasmid, pA51214VL'.
  • the modified cDNA was characterized by DNA sequencing.
  • the coding sequence for the 5.12.14 light chain is shown in Figure 19.
  • the DNA sequence between the end of the heavy chain variable region and the unique cloning site, Apal in the human IgGl heavy chain constant domain of pA51214VH was reconstructed to change the amino acids in this area from murine to human. This was done by the polymerase chain reaction. Amplification ofthe murine 5.12.14 heavy chain variable sequence was accomplished using the primers shown in Figure 18.
  • the forward PCR primer (SEQ ID NO: 22) was designed to match nucleotides 867-887 in pA51214VH upstream of the STII signal sequence and the putative cDNA sequence encoding the heavy chain variable region and included the unique cloning site Spel.
  • the reverse PCR primer (SEQ ID NO: 23) was designed to match the last four amino acids of the 5.12.14 heavy chain variable sequence and the first six amino acids corresponding to the human IgG 1 heavy constant sequence which also included the unique cloning site, Apal.
  • the PCR product encoding the modified 5.12.14 heavy chain variable sequence was then subcloned to the expression plasmid, pMHM24.2.28 in a two-part ligation.
  • the vector was digested with Spel- Apal and the Spel-Apal digested 5.12.14 PCR product encoding the heavy chain variable region was ligated into it to form the plasmid, pA51214VH'.
  • the modified cDNA was characterized by DNA sequencing. The coding sequence for the 5.12.14 heavy chain is shown in the DNA sequence (SEQ ID NO: 26) and amino acid sequence (SEQ ID NO: 27) of Figures 20A-20B.
  • the first expression plasmid, pantiIL-8.1, encoding the chimeric Fab of 5.12.14 was made by digesting pA51214VH' with EcoRV and Bpul 1021 to replace the EcoRV-Bpu 1 1021 fragment with a EcoRV- Bpul l02I fragment encoding the murine 5.12.14 light chain variable region of pA51214VL'.
  • the resultant plasmid thus contained the murine-human variable/constant regions of both the light and heavy chains of 5.12.14.
  • the second expression plasmid, pantiIL-8.2 was constructed using *he plasmid, pmyl87, as the vector. Plasmid pantiIL-8.2 was made by digesting pmyl 87 with Mlul and Sphl and the Mlul (partial)-Sphl fragment encoding the murine 5.12.14 murine-human chimeric Fab of pantiIL-8.1 was ligated into it. The resultant plasmid thus contained the murine-human variable/constant regions of both the light and heavy chains of 5.12.14.
  • the plasmid pantiIL-8.2 was deposited on February 10, 1995 with the American Type Culture Collection, 12301 Parklawn Drive, Rockville, MD, U.S.A. (ATCC) and assigned ATTC Accession No. ATCC 97056.
  • Three primers (SEQ ID NOS: SEQ ID NOS: 1-6) were designed for each the light and heavy chains to increase the chances of primer hybridization and efficiency of first strand cDNA synthesis (Figure 21).
  • Amplification of the first strand cDNA to double-stranded (ds) DNA was accomplished using two sets of synthetic DNA oligonucleotide primers: one forward primer (SEQ ID NOS: 28-30) and one reverse primer (SEQ ID NO: 31) for the light chain variable region amplification ( Figure 22) and one forward primer (SEQ ID NOS: 32-33) and one reverse primer (SEQ ID NOS: 1 1,15,14 and 13) for the heavy chain variable region amplification ( Figure 23).
  • the N-terminal sequence of the first eight amino acids of either the light or heavy chains of 6G4.2.5 was used to generate a putative murine DNA sequence corresponding to this region. (A total of 29 amino acids were sequenced from the N-terminus of both the light chain and heavy chain variable regions using the Edman degradation protein sequencing technique.) This information was used to design the forward amplification primers which were made degenerate in the third position for some codons to increase the chances of primer hybridization to the natural murine DNA codons and also included the unique restriction site, Nsil, for the light chain variable region forward primer and the unique restriction site, Mlul, for the heavy chain variable region forward primer to facilitate ligation to the 3' end of the STII element in the vector, pchimFab.
  • the reverse amplification primers were designed to anneal with the murine DNA sequence corresponding to a portion of the constant region of the light or heavy chains near the variable/constant junction.
  • the light chain variable region reverse primer contained a unique Muni restriction site and the heavy chain variable region reverse primer contained a unique Apal restriction site for ligation to the 5' end of either the human IgG 1 constant light or IgG 1 constant heavy regions in the vector, pchimFab.
  • the polymerase chain reaction using these primer sets yielded DNA fragments of approximately 400 bp and were cloned individually into the vector, pchimFab. to form p6G425VL and p6G425VH.
  • the cDNA inserts were characterized by DNA sequencing and are presented in the DNA sequence (SEQ ID NO: 34) and amino acid sequence (SEQ ID NO: 35) of Figure 24 (murine light chain variable region) and the DNA sequence (SEQ ID NO: 36) and amino acid sequence (SEQ ID NO: 37) of Figure 25 (murine heavy chain variable region).

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Abstract

La présente invention concerne l'utilisation d'anticorps monoclonaux humanisés anti-IL-8 et de leurs variantes à des fins de diagnostic et de traitement de maladies inflammatoires. L'invention concerne également un conjugué formé par un fragment d'anticorps lié par covalence à un polymère non protéinique, la taille apparente du conjugué étant au moins d'environ 500 kD. Ce conjugué marque une nette amélioration en termes de demi-vie, de temps de séjour moyen et/ou de rythme de clairance en circulation par rapport au fragment d'anticorps parental non transformé en dérivé.
PCT/US1999/001081 1998-01-22 1999-01-19 Conjugues fragments d'anticorps-polymeres et anticorps monoclonaux humanises anti-il-8, et leurs utilisations WO1999037779A1 (fr)

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US6458355B1 (en) 1998-01-22 2002-10-01 Genentech, Inc. Methods of treating inflammatory disease with anti-IL-8 antibody fragment-polymer conjugates
US6468532B1 (en) 1998-01-22 2002-10-22 Genentech, Inc. Methods of treating inflammatory diseases with anti-IL-8 antibody fragment-polymer conjugates
EP1290033A1 (fr) * 2000-06-05 2003-03-12 The Trustees Of Columbia University In The City Of New York Identification et utilisation des cellules progenitrices endotheliales derivees de la moelle osseuse, destinees a ameliorer la fonction du myocarde apres un accident ischemique
US6870033B1 (en) 1997-02-21 2005-03-22 Genentech, Inc. Antibody fragment-polymer conjugates and humanized anti-IL-8 monoclonal antibodies
US7005504B2 (en) 1998-01-22 2006-02-28 Genentech, Inc. Antibody fragment-peg conjugates
WO2006054059A1 (fr) * 2004-11-19 2006-05-26 Ucb Pharma S.A. Neutralisation de molecules d'anticorps presentant une specificite pour l'il-17 humaine
US7214776B2 (en) 1999-01-21 2007-05-08 Genentech, Inc. Antibody fragment-polymer conjugates and uses of same
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US9050304B2 (en) 2007-04-03 2015-06-09 Ratiopharm Gmbh Methods of treatment using glycopegylated G-CSF
US9150848B2 (en) 2008-02-27 2015-10-06 Novo Nordisk A/S Conjugated factor VIII molecules
US9187532B2 (en) 2006-07-21 2015-11-17 Novo Nordisk A/S Glycosylation of peptides via O-linked glycosylation sequences
US9187546B2 (en) 2005-04-08 2015-11-17 Novo Nordisk A/S Compositions and methods for the preparation of protease resistant human growth hormone glycosylation mutants
US9200049B2 (en) 2004-10-29 2015-12-01 Novo Nordisk A/S Remodeling and glycopegylation of fibroblast growth factor (FGF)
EP2305314B1 (fr) * 2001-10-10 2015-12-23 ratiopharm GmbH Remodelage et glycoconjugation des anticorps
US9321812B2 (en) * 2014-03-28 2016-04-26 Perle Bioscience Insulin independence among patients with diabetes utilizing an optimized hamster REG3 gamma peptide
US9493499B2 (en) 2007-06-12 2016-11-15 Novo Nordisk A/S Process for the production of purified cytidinemonophosphate-sialic acid-polyalkylene oxide (CMP-SA-PEG) as modified nucleotide sugars via anion exchange chromatography
US9777071B2 (en) 2013-12-12 2017-10-03 Abbvie Stemcentrx Llc Anti-DPEP3 antibodies and methods of use
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US10053511B2 (en) 2013-11-06 2018-08-21 Abbvie Stemcentrx Llc Anti-claudin antibodies and methods of use
US10308721B2 (en) 2014-02-21 2019-06-04 Abbvie Stemcentrx Llc Anti-DLL3 antibodies and drug conjugates for use in melanoma
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US10428156B2 (en) 2014-09-05 2019-10-01 Abbvie Stemcentrx Llc Anti-MFI2 antibodies and methods of use
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