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WO1999027131A3 - Topologically fixed matrix bonded nucleic acid molecule - Google Patents

Topologically fixed matrix bonded nucleic acid molecule Download PDF

Info

Publication number
WO1999027131A3
WO1999027131A3 PCT/DE1998/003466 DE9803466W WO9927131A3 WO 1999027131 A3 WO1999027131 A3 WO 1999027131A3 DE 9803466 W DE9803466 W DE 9803466W WO 9927131 A3 WO9927131 A3 WO 9927131A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid molecule
fixed matrix
bonded nucleic
matrix bonded
Prior art date
Application number
PCT/DE1998/003466
Other languages
German (de)
French (fr)
Other versions
WO1999027131A2 (en
Inventor
Claas Junghans
Burghardt Wittig
Original Assignee
Claas Junghans
Burghardt Wittig
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Claas Junghans, Burghardt Wittig filed Critical Claas Junghans
Priority to EP98963380A priority Critical patent/EP0975808A2/en
Publication of WO1999027131A2 publication Critical patent/WO1999027131A2/en
Publication of WO1999027131A3 publication Critical patent/WO1999027131A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention relates to nucleic acid molecules which are bonded on a surface in such a way that coiling energy or superhelicity cannot escape from said molecules. The invention also relates to a method with which such enzyme actions can be examined, said enzyme actions changing the topological state of DNA. Such nucleic acid molecules and methods can be utilized in pharmaceutical research in the automated search for cytostatics or antibiotics.
PCT/DE1998/003466 1997-11-21 1998-11-19 Topologically fixed matrix bonded nucleic acid molecule WO1999027131A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP98963380A EP0975808A2 (en) 1997-11-21 1998-11-19 Topologically fixed matrix bonded nucleic acid molecule

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19753182A DE19753182A1 (en) 1997-11-21 1997-11-21 Topologically fixed matrix-bound nucleic acid
DE19753182.2 1997-11-21

Publications (2)

Publication Number Publication Date
WO1999027131A2 WO1999027131A2 (en) 1999-06-03
WO1999027131A3 true WO1999027131A3 (en) 1999-10-07

Family

ID=7850347

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE1998/003466 WO1999027131A2 (en) 1997-11-21 1998-11-19 Topologically fixed matrix bonded nucleic acid molecule

Country Status (3)

Country Link
EP (1) EP0975808A2 (en)
DE (1) DE19753182A1 (en)
WO (1) WO1999027131A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001270098A1 (en) * 2000-06-22 2002-01-02 San Diego State University Foundation Recombination modulators and methods for their production and use
GB0424953D0 (en) * 2004-11-11 2004-12-15 Plant Bioscience Ltd Assay
US20080089932A1 (en) 2006-10-13 2008-04-17 Steffen Panzner Amphoteric liposomes, a method of formulating an amphoteric liposome and a method of loading an amphoteric liposome
JP2010513354A (en) 2006-12-19 2010-04-30 ノヴォソム アクチェンゲゼルシャフト Lipids and lipid aggregates containing transfection enhancer elements
DE102007029471A1 (en) 2007-06-20 2008-12-24 Novosom Ag New optional cationic sterols
CN102481256B (en) 2009-07-09 2015-02-25 玛瑞纳生物技术有限公司 Amphoteric liposomes comprising imino lipids

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0484472B1 (en) * 1990-04-12 1997-07-16 SIGRIST, Hans, Dr. Method for the light-induced immobilization of biomolecules on chemically "inert" surfaces
DE19648625A1 (en) * 1996-11-13 1998-05-14 Soft Gene Gmbh Microprojectile for the introduction of substances into cells by ballistic transfer
EP0972081B1 (en) * 1997-04-01 2007-06-13 Solexa Ltd. Method of nucleic acid amplification

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ARNDT-JOVIN D. ET AL.,: "Z-DNA binding and inhibition by GTP of Drosophila topoisomeraes II", BIOCHEMISTRY, vol. 32, - 1993, pages 4862 - 4872, XP002111474 *
GLIKIN G.C. ET AL.,: "Interactions of drosophila DNA topoisomerase II with left-handed Z-DNA in supercoiled minicircles", NUCLEIC ACIDS RESEARCH, vol. 19, no. 25, - 1991, pages 7139 - 7144, XP002111475 *
THOMAS T.J.& THOMAS T.: "Direct evidence for the presence of left-handed conformation in a supramolecular assembly of polynucleotides", NUCLEIC ACIDS RESEARCH, vol. 17, - 1989, pages 3795 - 3810, XP002111473 *

Also Published As

Publication number Publication date
EP0975808A2 (en) 2000-02-02
WO1999027131A2 (en) 1999-06-03
DE19753182A1 (en) 1999-07-29

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