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WO1999013064A3 - Viral infection of cells using viral vectors - Google Patents

Viral infection of cells using viral vectors Download PDF

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Publication number
WO1999013064A3
WO1999013064A3 PCT/GB1998/002753 GB9802753W WO9913064A3 WO 1999013064 A3 WO1999013064 A3 WO 1999013064A3 GB 9802753 W GB9802753 W GB 9802753W WO 9913064 A3 WO9913064 A3 WO 9913064A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
viral
infecting
infected
viral vector
Prior art date
Application number
PCT/GB1998/002753
Other languages
French (fr)
Other versions
WO1999013064A2 (en
Inventor
Marc Feldmann
Brian Maurice John Foxwell
Fionula Mary Brennan
Jan Bondeson
Original Assignee
Kennedy Rheumatology Inst
Marc Feldmann
Brian Maurice John Foxwell
Fionula Mary Brennan
Jan Bondeson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kennedy Rheumatology Inst, Marc Feldmann, Brian Maurice John Foxwell, Fionula Mary Brennan, Jan Bondeson filed Critical Kennedy Rheumatology Inst
Priority to EP98942892A priority Critical patent/EP1012321A2/en
Priority to JP2000510853A priority patent/JP2001515716A/en
Publication of WO1999013064A2 publication Critical patent/WO1999013064A2/en
Publication of WO1999013064A3 publication Critical patent/WO1999013064A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/04—Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A61P11/06—Antiasthmatics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Pulmonology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Pain & Pain Management (AREA)
  • Rheumatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

The invention relates to an in vitro method for infecting one or more cells with a viral vector capable of transporting exogenous or recombinant nucleic acid into the cells to be infected, comprising the steps of: a) collecting the cells to be infected; b) treating the collected cells with at least one cytokine; and c) infecting the collected cells with a viral vector such as adenovirus. A method of infecting rheumatoid synovial cells and other cells comprising high levels of cell surface integrins is also disclosed.
PCT/GB1998/002753 1997-09-11 1998-09-11 Viral infection of cells using viral vectors WO1999013064A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP98942892A EP1012321A2 (en) 1997-09-11 1998-09-11 Viral infection of cells using viral vectors
JP2000510853A JP2001515716A (en) 1997-09-11 1998-09-11 Viral infection of cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9719238.9 1997-09-11
GBGB9719238.9A GB9719238D0 (en) 1997-09-11 1997-09-11 Viral infection of cells

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US09508350 A-371-Of-International 2000-04-07
US10/033,267 Continuation US20020177572A1 (en) 1997-09-11 2001-10-25 Viral infection of cells

Publications (2)

Publication Number Publication Date
WO1999013064A2 WO1999013064A2 (en) 1999-03-18
WO1999013064A3 true WO1999013064A3 (en) 1999-06-17

Family

ID=10818857

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1998/002753 WO1999013064A2 (en) 1997-09-11 1998-09-11 Viral infection of cells using viral vectors

Country Status (4)

Country Link
EP (1) EP1012321A2 (en)
JP (1) JP2001515716A (en)
GB (1) GB9719238D0 (en)
WO (1) WO1999013064A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9922281D0 (en) * 1999-09-22 1999-11-17 Kennedy Rheumatology Inst Polymorphism
GB9930616D0 (en) 1999-12-24 2000-02-16 Mathilda & Terence Kennedy Ins Activation and inhibition of the immune system
US7105336B2 (en) * 2002-10-07 2006-09-12 Biogaia Ab Selection and use of lactic acid bacteria for reducing inflammation caused by Helicobacter

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0779361A2 (en) * 1995-12-15 1997-06-18 F. Hoffmann-La Roche Ag Truncated form of inhibitory kappa B protein (1kB), recombinant production and uses thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0779361A2 (en) * 1995-12-15 1997-06-18 F. Hoffmann-La Roche Ag Truncated form of inhibitory kappa B protein (1kB), recombinant production and uses thereof

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
B. FOXWELL ET AL.,: "Efficient adenoviral infection with IkappaB alpha reveals that macrophage tumor necrosis alpha production in rheumatoid arthritis is NF-kappaB dependent", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 95, no. 14, 1998, pages 8211 - 8215, XP002098099 *
BAEUERLE P A ET AL: "FUNCTION AND ACTIVATION OF NF-KappaB INTHE IMMUNE SYSTEM", ANNUAL REVIEW OF IMMUNOLOGY, vol. 12, 1994, pages 141 - 179, XP000612175 *
C. JOBIN ET AL.,: "Inhibition of proinflammatory molecule production by adenovirus-mediated expression of a nuclear factor kappa-beta super-repressor in human intestinal epithelial cells.", JOURNAL OF INMUNOLOGY, vol. 160, no. 1, 1 January 1998 (1998-01-01), pages 410 - 418, XP002098101 *
C. JOBIN ET AL.,: "Potential novel therapy for the intestine: adenoviral mediated gene delivery of an NF-kappa super-repressor blocked proinflammatory gene expression in human intestinal epithelial cells", GASTROENTEROLOGY, vol. 112, no. (4 suppl.), 1997, pages pA1006, XP002098097 *
HADDADA H ET AL: "EFFICIENT ADENOVIRUS-MEDIATED GENE TRANSFER INTO HUMAN BLOOD MONOCYTE-DERIVED MACROPHAGES", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 195, no. 3, 30 September 1993 (1993-09-30), pages 1174 - 1183, XP002005682 *
H-G ZHANG ET AL.,: "Increased susceptibility of RA synovial cells to TNFalpha-mediated apoptosis after IKB dominate negative gene therapy", ARTHRITIS AND RHEUMATISM, vol. 41, no. (9 suppl.), 1 September 1998 (1998-09-01), pages ps96, XP002098100 *
M. FELDMAN ET AL.,: "Anti-TNFalpha therapy is useful in rheumatoid arthritis and Crohn's disease: analysis of the mechanism of action predicts utility in other diseases", TRANSPLANTATION PROCEEDINGS, vol. 30, no. 8, 1 December 1998 (1998-12-01), pages 4126 - 4127, XP002098301 *
PAHL H L ET AL: "The ER-overload response: activation of NF-kappaB", TIBS TRENDS IN BIOCHEMICAL SCIENCES, vol. 22, no. 2, February 1997 (1997-02-01), pages 63-67, XP004050133 *
S. HUANG ET AL.,: "Upregulation of integrins alpha-v-beta-3 and alpha-v-beta-5 on human monocytes and T lymphocytes facilitates adenovirus-mediated gene delivery", JOURNAL OF VIROLOGY, vol. 69, no. 4, 1995, pages 2257 - 2263, XP002098096 *
S.S. MAKAROV ET AL.,: "NF-kappa B as a target for anti-inflammatory gene therapy: Suppression of inflammatory responses in monocytic and stromal cells by stable gene transfer of I-kappa-B-alpha cDNA", GENE THERAPY, vol. 4, no. 8, 1997, pages 846 - 852, XP002098098 *

Also Published As

Publication number Publication date
WO1999013064A2 (en) 1999-03-18
GB9719238D0 (en) 1997-11-12
EP1012321A2 (en) 2000-06-28
JP2001515716A (en) 2001-09-25

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