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WO1999004014A3 - Transformed yeast strains and their use for the production of monoterminal and diterminal aliphatic carboxylates - Google Patents

Transformed yeast strains and their use for the production of monoterminal and diterminal aliphatic carboxylates Download PDF

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Publication number
WO1999004014A3
WO1999004014A3 PCT/US1998/014935 US9814935W WO9904014A3 WO 1999004014 A3 WO1999004014 A3 WO 1999004014A3 US 9814935 W US9814935 W US 9814935W WO 9904014 A3 WO9904014 A3 WO 9904014A3
Authority
WO
WIPO (PCT)
Prior art keywords
monoterminal
diterminal
production
yeast strains
genetically
Prior art date
Application number
PCT/US1998/014935
Other languages
French (fr)
Other versions
WO1999004014A2 (en
Inventor
Robert D Fallon
Mark S Payne
Stephen K Picataggio
Shijun Wu
Original Assignee
Du Pont
Robert D Fallon
Mark S Payne
Stephen K Picataggio
Shijun Wu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Robert D Fallon, Mark S Payne, Stephen K Picataggio, Shijun Wu filed Critical Du Pont
Priority to CA002293737A priority Critical patent/CA2293737A1/en
Priority to EP98935806A priority patent/EP1003881A2/en
Priority to JP2000503220A priority patent/JP2001510048A/en
Priority to IL13393198A priority patent/IL133931A0/en
Priority to AU84982/98A priority patent/AU8498298A/en
Publication of WO1999004014A2 publication Critical patent/WO1999004014A2/en
Publication of WO1999004014A3 publication Critical patent/WO1999004014A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/165—Yeast isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/72—Candida
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/84—Pichia

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention comprises a bioprocess for converting aliphatic compounds, of the form CH3(CH2)nCH3 where n = 4 to 20, to monoterminal and diterminal carboxylates using genetically-engineered organisms. This invention relates to a process for expressing alkane hydroxylating activity in genetically-engineered yeasts Pichia pastoris and Candida maltosa. In addition, the present invention describes a process to produce genetically transformed Candida maltosa strains that have enhanced cytochrome P450 activity and/or gene disruptions in the β-oxidation pathway.
PCT/US1998/014935 1997-07-21 1998-07-20 Transformed yeast strains and their use for the production of monoterminal and diterminal aliphatic carboxylates WO1999004014A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002293737A CA2293737A1 (en) 1997-07-21 1998-07-20 Transformed yeast strains and their use for the production of monoterminal and diterminal aliphatic carboxylates
EP98935806A EP1003881A2 (en) 1997-07-21 1998-07-20 Transformed yeast strains and their use for the production of monoterminal and diterminal aliphatic carboxylates
JP2000503220A JP2001510048A (en) 1997-07-21 1998-07-20 Transformed yeast strain and its use for producing mono- and di-terminal aliphatic carboxylate
IL13393198A IL133931A0 (en) 1997-07-21 1998-07-20 Transformed yeast strains and their use for the production of monoterminal and diterminal aliphatic carboxylates
AU84982/98A AU8498298A (en) 1997-07-21 1998-07-20 Transformed yeast strains and their use for the production of monoterminal and diterminal aliphatic carboxylates

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5321597P 1997-07-21 1997-07-21
US60/053,215 1997-07-21

Publications (2)

Publication Number Publication Date
WO1999004014A2 WO1999004014A2 (en) 1999-01-28
WO1999004014A3 true WO1999004014A3 (en) 1999-05-20

Family

ID=21982693

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/014935 WO1999004014A2 (en) 1997-07-21 1998-07-20 Transformed yeast strains and their use for the production of monoterminal and diterminal aliphatic carboxylates

Country Status (6)

Country Link
EP (1) EP1003881A2 (en)
JP (1) JP2001510048A (en)
AU (1) AU8498298A (en)
CA (1) CA2293737A1 (en)
IL (1) IL133931A0 (en)
WO (1) WO1999004014A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU5060200A (en) * 1999-04-24 2000-11-10 Max-Delbruck-Centrum Fur Molekulare Medizin Nucleic acid sequences from (candida) yeasts which code cytochrome b5 polypeptides
DE10036491A1 (en) 2000-07-25 2002-02-07 Roche Diagnostics Gmbh Expression of alkaline phosphatase in yeast
WO2010068904A2 (en) * 2008-12-12 2010-06-17 E. I. Du Pont De Nemours And Company Process for making linear dicarboxylic acids from renewable resources
DE102010015807A1 (en) * 2010-04-20 2011-10-20 Evonik Degussa Gmbh Biocatalytic oxidation process with alkL gene product
US8728798B2 (en) 2011-05-03 2014-05-20 Verdezyne, Inc. Biological methods for preparing adipic acid
EP2729564B1 (en) 2011-07-06 2019-09-18 Radici Chimica S.p.A. Biological methods for preparing a fatty dicarboxylic acid
EP3173478A1 (en) * 2015-11-25 2017-05-31 Evonik Degussa GmbH Biotechnological production of omega-functionalised carboxylic acids and esters thereof
IT201700091114A1 (en) * 2017-08-09 2019-02-09 Novamont Spa CANDIDA GENETICALLY MODIFIED FOR THE PRODUCTION OF DICARBOSSYLIC FATTY ACIDS
CN110684809A (en) * 2018-07-06 2020-01-14 上海凯赛生物技术股份有限公司 Twelve-carbon dicarboxylic acid product produced by fermentation method and preparation method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2069291A5 (en) * 1969-11-10 1971-09-03 Ajinomoto Kk
EP0296506A2 (en) * 1987-06-26 1988-12-28 Henkel Kommanditgesellschaft auf Aktien Fermentative production of dicarboxylic acids
WO1991006660A1 (en) * 1989-11-06 1991-05-16 Henkel Research Corporation Site-specific modification of the candida tropicalis genome
DE19507546A1 (en) * 1995-03-03 1996-09-12 Max Delbrueck Centrum Process for the hydroxylation of long chain alkanes, fatty acids and other alkyl compounds

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2069291A5 (en) * 1969-11-10 1971-09-03 Ajinomoto Kk
EP0296506A2 (en) * 1987-06-26 1988-12-28 Henkel Kommanditgesellschaft auf Aktien Fermentative production of dicarboxylic acids
WO1991006660A1 (en) * 1989-11-06 1991-05-16 Henkel Research Corporation Site-specific modification of the candida tropicalis genome
US5254466A (en) * 1989-11-06 1993-10-19 Henkel Research Corporation Site-specific modification of the candida tropicals genome
DE19507546A1 (en) * 1995-03-03 1996-09-12 Max Delbrueck Centrum Process for the hydroxylation of long chain alkanes, fatty acids and other alkyl compounds

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ISAMU SHIIO ET AL.: "Microbial production of long-chain dicarboxylic acids from n-alkanes", AGRICULTURAL AND BIOLOGICAL CHEMISTRY, vol. 35, no. 13, 1971, TOKYO JP, pages 2033 - 2042, XP002084652 *
KARGEL E ET AL: "Candida maltosa NADPH-cytochrome P450 reductase: cloning of a full-length cDNA, heterologous expression in Saccharomyces cerevisiae and function of the N-terminal region for membrane anchoring and proliferation of the endoplasmic reticulum.", YEAST, (1996 MAR 30) 12 (4) 333-48. JOURNAL CODE: YEA. ISSN: 0749-503X., ENGLAND: United Kingdom, XP000608022 *
PARK S M ET AL: "Galactose-inducible expression systems in Candida maltosa using promoters of newly-isolated GAL1 and GAL1 genes.", YEAST, (1997 JAN) 13 (1) 21-9. JOURNAL CODE: YEA. ISSN: 0749-503X., ENGLAND: United Kingdom, XP002093562 *

Also Published As

Publication number Publication date
WO1999004014A2 (en) 1999-01-28
EP1003881A2 (en) 2000-05-31
IL133931A0 (en) 2001-04-30
CA2293737A1 (en) 1999-01-28
AU8498298A (en) 1999-02-10
JP2001510048A (en) 2001-07-31

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