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WO1998049340A1 - Dispositif et procede d'etablissement de cycle - Google Patents

Dispositif et procede d'etablissement de cycle Download PDF

Info

Publication number
WO1998049340A1
WO1998049340A1 PCT/AU1998/000277 AU9800277W WO9849340A1 WO 1998049340 A1 WO1998049340 A1 WO 1998049340A1 AU 9800277 W AU9800277 W AU 9800277W WO 9849340 A1 WO9849340 A1 WO 9849340A1
Authority
WO
WIPO (PCT)
Prior art keywords
rotor
dna
reaction
sample
reaction mixture
Prior art date
Application number
PCT/AU1998/000277
Other languages
English (en)
Inventor
John Michael Corbett
John Michael Corbett, Jr.
Original Assignee
John Michael Corbett
Corbett John Michael Jr
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by John Michael Corbett, Corbett John Michael Jr filed Critical John Michael Corbett
Priority to AU70136/98A priority Critical patent/AU7013698A/en
Publication of WO1998049340A1 publication Critical patent/WO1998049340A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces

Definitions

  • the present invention relates to a device and method for the rapid cycling of a reaction mix between various temperatures
  • the device and method are particularly suitable for conducting DNA polymerase or ligase chain reactions, however, can be used for a range of enzymatic reactions, which involve changes of temperature
  • a duplex DNA segment of up to approximate six thousand Base repairs in length may be amplified many million fold by means of the polymerase chain reaction (PCR), starting from as little as a single copy.
  • PCR polymerase chain reaction
  • a denature duplex DNA sample is incubated with a molar excess of two oligonucleotide primers, one being complimentary to a short strand of the DNA duplex and the other being identical to a second short sequence upstream of it (i e more 5'), such that each primer anneals to its complimentary sequence and primes the template-dependent synthesis by DNA polymerase of a complimentary strand which extends beyond the site of annealing of the other primer, through the incorporation of deoxynucleotide triphosphats provided Multiple cycles of denaturation, annealing and synthesis each afford an approximate doubling of the amount of target sequence, where the target sequence is defined as the DNA sequence subtended by and including the primers Each cycle is controlled by varying the temperature to permit successive denaturation of complimentary strands of duplex DNA, annealing of the primers to their complimentary sequences and a prime synthesis of new complimentary sequences and a prime synthesis of new complimentary DNA strands.
  • thermostable DNA polymerase obviates the necessity of adding new enzyme for each cycle, thus allowing automation of the DNA amplification process by thermal cycling Twenty amplification cycles increases the amount of target sequence by approximately one million-fold (being theoretically 220). More detailed information regarding the polymerase chain reaction can be found "PCR Protocols - A Guide to Methods and Application” Eds. M.A. Innis, D. H. Gelfard, J.J. Sainskey, TJ. White, Academic Press, Inc. San Diego 1990, the disclosure of which is incorporated herein by reference. In the prior art it has been found that the technique of DNA polymerization requires rapid controlled heating and cooling cycles. The art is replete with incubators and other devices to achieve this end, e.g. PCT/AU90/00560.
  • a device used for PCR consists of a heat conductive material provided with channels adapted to receive vessels in which the reaction is to take place,typically Eppendorf tubes. The heat conductive material is then provided with heating/cooling means.
  • the present inventors have developed a novel device and method, which allows the rapid cycling of a reaction mixture between various temperatures.
  • the present invention consists in a device comprising a chamber containing a rotor provided with a plurality of reaction zones and a plurality of sample loading zones, drive means to drive the rotor thereby applying a centrifugal force to the sample tubes, heating means to raise the temperature within the chamber, and openings through which a coolant gas can be admitted into the chamber.
  • the heating means are positioned within the chamber and will typically be infrared lights, convection heating elements or microwave sources. It is presently preferred, however, that the heating means are convection heating elements.
  • the heating means need not be positioned within the chamber, for example, the heating means may comprise a hot air blower from which a stream of heated air is directed into the chamber.
  • the coolant gas can be any of a large number of gasses, however, for the sake of convenience and cost effectiveness in most cases the coolant gas will simply be air at ambient temperature. It is, however, possible that in some situations refrigerated air may be used. This may be particularly beneficial where, after the required number of cycles have been conducted, it is desirous to cool the samples to sub-ambient temperatures.
  • means to open and close the openings are provided.
  • the device will include means for automatically actuating the heating means when the openings are closed and, after a predetermined time, turning off the heating means and opening the openings.
  • the openings are preferably provided in the top of the chamber and in the side walls of the chamber.
  • Each opening provided in the side wall of the chamber is positioned directly opposite another opening in the side wall of the chamber so that while the rotor is spinning at a constant speed there is no movement of air from the chamber.
  • the reaction zones are glass capillary tubes and the sample loading zones are slots.
  • the sample loading zones are slots.
  • the loading slots will be in fluid communication with the reaction zone such that upon actuation of the heating means will rapidly heat the contents of the sample within the reaction zone to the required temperature.
  • An increase in the speed of the rotor will result in air being drawn into the chamber through the openings displacing the heated air previously with the chamber. This will result in a rapid cooling of the contents of the reaction zone.
  • the device of the present invention will enable the rapid cycling between the temperatures routinely used in carrying out the polymerase chain reaction and other enzymatic reactions.
  • the use of the device of the present invention will, therefore, provide an effective method of cycling a reaction mixture between various temperatures.
  • monitoring means to assess the progress of the reaction occurring in the sample tube within the chamber.
  • this monitoring means will be a fluorescence detector, spectrophotometer, or photometer. This would be particularly useful in monitoring the progress of a number of enzymatic reactions where a change in optical density or fluorescence of the product is observed.
  • Such monitoring means would also be very useful in monitoring PCR reactions.
  • an intercalating dye such as Ethidium bromide or SYBR® Green, would be added to the reaction mix. When the dye binds to double stranded DNA there is fluorescence. Accordingly, by monitoring the degree of fluorescence in the reaction mixture an assessment as to the number of doublings, which have occurred, can be made.
  • fluorescently labeled probes that hybridize to the DNA could be used.
  • the cycling between various predetermined temperatures can be automated. This would involve actuation of the heating means, turning off the heating means and increasing the speed of the rotor, decreasing the speed of the rotor and reactivating the heating means. Further, in the situation where monitoring means are provided in the chamber when the reaction has reached a suitable point refrigerated gas may be pumped into the chamber thereby cooling the reaction mixture to a sub-ambient temperature.
  • the present invention consists in a method of cycling a reaction mixture between predetermined temperatures, the method involving the use of the device of the first aspect of the present invention in which samples are placed in the sample loading zones, the drive means is actuated thereby spinning the rotor causing the samples to travel to the reaction zones and mix, and cyclically actuating the heating means, ceasing heating and introducing a coolant gas into the chamber via the openings.
  • FIG 3 shows a schematic view of the device.
  • the rotor consists of a high temperature plastic with a number of slots (1) and loading wells (2a, 2b) positioned radically. At the end of each slot there is provide a glass capillary tube (3) with the outer end sealed and the inner end open to the slot. Reagents are pipetted into the loading wells, (2a, 2b) which are positioned around the inner radius of the rotor. Reagent mixture and DNA sample are separated during loading and are only mixed together when the rotor is spun, thus avoiding any hybridization before thermal cycling commences.
  • the rotor consists of a high temperature plastic disc, which consists of upper and lower sections welded together.
  • the lower section contains a formed slot (1) with two or more loading wells formed at the inner radius (2a, 2b).
  • the dimensions of the rotor disc are preferably identical to that of an audio Compact Disk.
  • the lower section also has a reaction well (3) where the samples are monitored during the run.
  • the upper section of the rotor has two or more holes over the loading wells, (2a, 2b) to allow reaction mixture and DNA sample to be loaded, and sealed over the reaction well to form a closed vessel.
  • reaction mixture is loaded into the loading wells (2a) and DNA sample is loaded into (2b), a cover is the clamped over the loaded wells (2a, 2b) and the rotor is the slowly rotated and brought to denaturation temperature. The rotor is then accelerated to a higher speed, sample and reagents move rapidly by centrifugal force into the reaction vessel at the outer diameter of the disc. The disc is then temperature cycled to initiate DNA amplification. While spinning the reaction products are monitored by a real time fluorescence optical system and the resulting data transferred to a computer analysis.
  • the device consists of an insulated base plate (1) with an insulated lid (2) containing the rotor (3) described above.
  • the rotor (3) is driven by motor (4) which is controlled by computer (5).
  • the position sensor (6) signals the computer when each reaction vessel is over the fluorescence detection system (7).
  • the data acquired by the fluorescence detector is transferred to the computer for analysis.
  • a heater (8) is placed in a duct which is fed by a fan (9), the air directed over the spinning rotor and exits via a duct (10).
  • the rotor temperature is detected by a sensor (11) and controlled by the computer system (5).

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

La présente invention concerne un appareil et un procédé permettant l'amplification et la détection en temps réel de fragments d'ADN spécifiques grâce à l'utilisation de la réaction en chaîne de la polymérase, de la réaction en chaîne de la ligase ou de toute autre technique d'amplification ou d'hybridation. Le procédé selon la présente invention consiste à charger de manière centrifuge des échantillons d'ADN et des réactifs à l'intérieur d'un rotor qui est fermé de façon étanche en vue d'éliminer toute contamination. Ledit rotor scellé est alors chauffé et/ou refroidi. Un élément de détection contrôle la fluorescence de chaque échantillon au cours des transitions de température. Le procédé selon la présente invention permet un traitement rapide d'un grand nombre d'échantillons.
PCT/AU1998/000277 1997-04-30 1998-04-21 Dispositif et procede d'etablissement de cycle WO1998049340A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU70136/98A AU7013698A (en) 1997-04-30 1998-04-21 Temperature cycling device and method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AUPO6529A AUPO652997A0 (en) 1997-04-30 1997-04-30 Temperature cycling device and method
AUPO6529 1997-04-30

Publications (1)

Publication Number Publication Date
WO1998049340A1 true WO1998049340A1 (fr) 1998-11-05

Family

ID=3800821

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/AU1998/000277 WO1998049340A1 (fr) 1997-04-30 1998-04-21 Dispositif et procede d'etablissement de cycle

Country Status (2)

Country Link
AU (1) AUPO652997A0 (fr)
WO (1) WO1998049340A1 (fr)

Cited By (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2812306A1 (fr) * 2000-07-28 2002-02-01 Gabriel Festoc Systeme d'amplification en chaine par polymerse de sequences nucleiques cibles
EP1325306A1 (fr) * 2001-04-24 2003-07-09 Dade MicroScan Inc. Dispositif de test d'identification dans un analyseur microbiologique a acces direct
US6627159B1 (en) 2000-06-28 2003-09-30 3M Innovative Properties Company Centrifugal filling of sample processing devices
WO2003102226A1 (fr) * 2002-05-30 2003-12-11 Bio-Molecular Holdings Pty Limited Procede et dispositif permettant une meilleure amplification de l'adn
EP1379852A1 (fr) * 2001-04-18 2004-01-14 3M Innovative Properties Company Dispositifs, procede et systemes de traitement d'echantillons multi-format
US6734401B2 (en) 2000-06-28 2004-05-11 3M Innovative Properties Company Enhanced sample processing devices, systems and methods
WO2004046719A1 (fr) 2002-11-20 2004-06-03 Bio-Molecular Holdings Pty Limited Dispositif centrifuge et procede permettant d'effectuer des analyses par liaison
US6783993B1 (en) 1999-03-25 2004-08-31 Alphahelix Ab Homogenizing of small-volume mixtures by centrifugation and heating
GB2399776A (en) * 2003-03-24 2004-09-29 Pa Knowledge Ltd Cyclical heating and cooling device and associated methods
WO2004087950A3 (fr) * 2003-04-04 2004-11-25 Roche Diagnostics Gmbh Système amélioré de pcr en temps réel multicolore
EP1551949A1 (fr) * 2002-05-01 2005-07-13 Corbett Life Science Pty Ltd Dispositif permettant d'amplifier l'adn, comprenant une source d'energie par micro-ondes
US7323660B2 (en) 2005-07-05 2008-01-29 3M Innovative Properties Company Modular sample processing apparatus kits and modules
US7332129B2 (en) 2003-01-09 2008-02-19 3M Innovative Properties Company Sample processing device having process chambers with bypass slots
USD564667S1 (en) 2005-07-05 2008-03-18 3M Innovative Properties Company Rotatable sample processing disk
WO2008102772A1 (fr) * 2007-02-22 2008-08-28 Toyo Boseki Kabushiki Kaisha Système d'amplification d'acide nucléique
WO2008106719A1 (fr) * 2007-03-02 2008-09-12 Corbett Research Pty Ltd Appareil et procédé d'amplification d'acide nucléique
US7449326B2 (en) 2000-02-11 2008-11-11 Roche Diagnostics Operations, Inc. System for simple nucleic acid analysis
AU2003227117B2 (en) * 2002-05-01 2009-01-15 Corbett Life Science Pty Ltd Device for the amplification of DNA, comprising a microwave energy source
US7537917B2 (en) 2006-03-31 2009-05-26 Collins Michael J Microwave assisted PCR amplification of DNA
AU2003283064B2 (en) * 2002-11-20 2009-06-04 Qiagen Gmbh Centrifugal device and method for performing binding assays
WO2009067744A1 (fr) 2007-11-30 2009-06-04 Corbett Research Pty Ltd Dispositif d'application de cycles thermiques
US7569186B2 (en) 2001-12-28 2009-08-04 3M Innovative Properties Company Systems for using sample processing devices
EP2107125A1 (fr) * 2008-03-31 2009-10-07 Eppendorf Array Technologies SA (EAT) PCR en temps réel de cibles sur un micro-réseau
US7754474B2 (en) 2005-07-05 2010-07-13 3M Innovative Properties Company Sample processing device compression systems and methods
US7763210B2 (en) 2005-07-05 2010-07-27 3M Innovative Properties Company Compliant microfluidic sample processing disks
WO2011042426A1 (fr) 2009-10-05 2011-04-14 Alphahelix Molecular Diagnostics Ab (Publ) Rotor multifonctionnel
US20110124002A1 (en) * 1999-07-16 2011-05-26 Life Technologies Corporation Fluid Processing Device Comprising Radial Channels
WO2011130785A1 (fr) 2010-04-20 2011-10-27 Corbett Research Pty Ltd Procédé et appareil de commande de la température
WO2012037978A1 (fr) * 2010-09-24 2012-03-29 Alphahelix Molecular Diagnostics Ab Nouveaux dispositif et procédé pour la mise en oeuvre d'une pcr quantitative directe en temps réel
US20120282708A1 (en) * 2009-07-29 2012-11-08 Pyrobett Pte Ltd. Method and Apparatus for Conducting an Assay
US8345215B2 (en) 2002-05-30 2013-01-01 Corbett Research Pty Ltd Optical means for calibrating temperature
WO2013057308A2 (fr) 2011-10-20 2013-04-25 Qiagen Instruments Ag Procédé pour vérifier une mesure de température dans un micro-environnement et système pour vérifier une mesure de température dans un micro-environnement
WO2013139970A1 (fr) 2012-03-23 2013-09-26 Qiagen Instruments Ag Thermocycleur et procédé de chauffage et de refroidissement
US8597882B2 (en) 2012-02-03 2013-12-03 Pyrobett Pte. Ltd. Method and apparatus for conducting an assay
EP2687294A1 (fr) 2012-07-18 2014-01-22 Qiagen Instruments AG Rotor pour cycleur thermique et cycleur thermique
US8834792B2 (en) 2009-11-13 2014-09-16 3M Innovative Properties Company Systems for processing sample processing devices
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US9067205B2 (en) 2011-05-18 2015-06-30 3M Innovative Properties Company Systems and methods for valving on a sample processing device
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US20180071728A1 (en) * 2016-09-12 2018-03-15 Delta Electronics Int'l (Singapore) Pte Ltd Nucleic acid analysis apparatus
US20180214877A1 (en) * 2016-09-12 2018-08-02 Delta Electronics Int'l (Singapore) Pte Ltd Nucleic acid analysis apparatus
CN109482246A (zh) * 2017-09-11 2019-03-19 台达电子国际（新加坡）私人有限公司 核酸分析装置
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CN109486667A (zh) * 2017-09-11 2019-03-19 台达电子国际（新加坡）私人有限公司 流体控制及处理卡匣
CN111263660A (zh) * 2017-04-17 2020-06-09 株式会社革新图案 利用离心力的数字pcr装置和方法
CN113913503A (zh) * 2021-08-25 2022-01-11 重庆大学 应用于核酸扩增技术的快速加热或冷却的温控方法
US11376581B2 (en) 2016-09-12 2022-07-05 Delta Electronics Int'l (Singapore) Pte Ltd Flow control and processing cartridge
US11426735B2 (en) 2016-09-12 2022-08-30 Delta Electronics Int'l (Singapore) Pte Ltd Nucleic acid analysis apparatus
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US6783993B1 (en) 1999-03-25 2004-08-31 Alphahelix Ab Homogenizing of small-volume mixtures by centrifugation and heating
US20110124002A1 (en) * 1999-07-16 2011-05-26 Life Technologies Corporation Fluid Processing Device Comprising Radial Channels
US7449326B2 (en) 2000-02-11 2008-11-11 Roche Diagnostics Operations, Inc. System for simple nucleic acid analysis
US7026168B2 (en) 2000-06-28 2006-04-11 3M Innovative Properties Company Sample processing devices
US7939018B2 (en) 2000-06-28 2011-05-10 3M Innovative Properties Company Multi-format sample processing devices and systems
US7445752B2 (en) 2000-06-28 2008-11-04 3M Innovative Properties Company Sample processing devices and carriers
US6734401B2 (en) 2000-06-28 2004-05-11 3M Innovative Properties Company Enhanced sample processing devices, systems and methods
US6627159B1 (en) 2000-06-28 2003-09-30 3M Innovative Properties Company Centrifugal filling of sample processing devices
US6987253B2 (en) 2000-06-28 2006-01-17 3M Innovative Properties Company Enhanced sample processing devices, systems and methods
US7595200B2 (en) 2000-06-28 2009-09-29 3M Innovative Properties Company Sample processing devices and carriers
US7435933B2 (en) 2000-06-28 2008-10-14 3M Innovative Properties Company Enhanced sample processing devices, systems and methods
US6814935B2 (en) 2000-06-28 2004-11-09 3M Innovative Properties Company Sample processing devices and carriers
US7164107B2 (en) 2000-06-28 2007-01-16 3M Innovative Properties Company Enhanced sample processing devices, systems and methods
US6821771B2 (en) 2000-07-28 2004-11-23 Genesystems Device for thermo-dependent chain reaction amplification of target nucleic acid sequences, measured in real-time
FR2812306A1 (fr) * 2000-07-28 2002-02-01 Gabriel Festoc Systeme d'amplification en chaine par polymerse de sequences nucleiques cibles
EP2269738A1 (fr) * 2000-07-28 2011-01-05 Genesystems Dispositif pour l'amplification en chaîne thermo-dépendante de séquences d'acides nucléiques cibles
US7732136B2 (en) 2000-07-28 2010-06-08 Pall Genesystems Device for thermo-dependent chain reaction amplification of target nucleic acid sequences, measured in real-time
WO2002009877A1 (fr) * 2000-07-28 2002-02-07 Genesystems Dispositif pour l'amplification en châine thermo-dependante de sequences d'acides nucleiques cibles
EP1729105A1 (fr) * 2001-04-18 2006-12-06 3M Innovative Properties Company Dispositifs, procédés et systèmes de traitement d'échantillon multiformat
EP1379852A4 (fr) * 2001-04-18 2004-11-17 3M Innovative Properties Co Dispositifs, procede et systemes de traitement d'echantillons multi-format
EP1379852A1 (fr) * 2001-04-18 2004-01-14 3M Innovative Properties Company Dispositifs, procede et systemes de traitement d'echantillons multi-format
EP1325306A1 (fr) * 2001-04-24 2003-07-09 Dade MicroScan Inc. Dispositif de test d'identification dans un analyseur microbiologique a acces direct
EP1325306A4 (fr) * 2001-04-24 2004-08-11 Dade Behring Inc Dispositif de test d'identification dans un analyseur microbiologique a acces direct
US8003051B2 (en) 2001-12-28 2011-08-23 3M Innovative Properties Company Thermal structure for sample processing systems
US7569186B2 (en) 2001-12-28 2009-08-04 3M Innovative Properties Company Systems for using sample processing devices
EP1551949A1 (fr) * 2002-05-01 2005-07-13 Corbett Life Science Pty Ltd Dispositif permettant d'amplifier l'adn, comprenant une source d'energie par micro-ondes
AU2003227117B2 (en) * 2002-05-01 2009-01-15 Corbett Life Science Pty Ltd Device for the amplification of DNA, comprising a microwave energy source
EP1551949A4 (fr) * 2002-05-01 2010-08-04 Corbett Life Science Pty Ltd Dispositif permettant d'amplifier l'adn, comprenant une source d'energie par micro-ondes
US8345215B2 (en) 2002-05-30 2013-01-01 Corbett Research Pty Ltd Optical means for calibrating temperature
WO2003102226A1 (fr) * 2002-05-30 2003-12-11 Bio-Molecular Holdings Pty Limited Procede et dispositif permettant une meilleure amplification de l'adn
AU2003229394B2 (en) * 2002-05-30 2008-11-20 Qiagen Instruments Ag Improved DNA amplification apparatus and method
US9089851B2 (en) 2002-05-30 2015-07-28 Qiagen Instruments Ag DNA amplification apparatus and method
AU2009200693B2 (en) * 2002-05-30 2011-03-03 Qiagen Instruments Ag Improved DNA amplification apparatus and method
EP1567861A4 (fr) * 2002-11-20 2008-04-09 Corbett Life Science Pty Ltd Dispositif centrifuge et procede permettant d'effectuer des analyses par liaison
WO2004046719A1 (fr) 2002-11-20 2004-06-03 Bio-Molecular Holdings Pty Limited Dispositif centrifuge et procede permettant d'effectuer des analyses par liaison
AU2003283064B2 (en) * 2002-11-20 2009-06-04 Qiagen Gmbh Centrifugal device and method for performing binding assays
EP1567861A1 (fr) * 2002-11-20 2005-08-31 Corbett Life Science Pty Ltd Dispositif centrifuge et procede permettant d'effectuer des analyses par liaison
US7332129B2 (en) 2003-01-09 2008-02-19 3M Innovative Properties Company Sample processing device having process chambers with bypass slots
GB2399776A (en) * 2003-03-24 2004-09-29 Pa Knowledge Ltd Cyclical heating and cooling device and associated methods
WO2004087950A3 (fr) * 2003-04-04 2004-11-25 Roche Diagnostics Gmbh Système amélioré de pcr en temps réel multicolore
US8137616B2 (en) 2003-04-04 2012-03-20 Roche Molecular Systems, Inc. System for multi color real time PCR
EP2284278A1 (fr) * 2003-04-04 2011-02-16 Roche Diagnostics GmbH Système amélioré pour PCR multicolore en temps réel
US7754474B2 (en) 2005-07-05 2010-07-13 3M Innovative Properties Company Sample processing device compression systems and methods
US7763210B2 (en) 2005-07-05 2010-07-27 3M Innovative Properties Company Compliant microfluidic sample processing disks
US7767937B2 (en) 2005-07-05 2010-08-03 3M Innovative Properties Company Modular sample processing kits and modules
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USD564667S1 (en) 2005-07-05 2008-03-18 3M Innovative Properties Company Rotatable sample processing disk
US7323660B2 (en) 2005-07-05 2008-01-29 3M Innovative Properties Company Modular sample processing apparatus kits and modules
US7537917B2 (en) 2006-03-31 2009-05-26 Collins Michael J Microwave assisted PCR amplification of DNA
WO2008102772A1 (fr) * 2007-02-22 2008-08-28 Toyo Boseki Kabushiki Kaisha Système d'amplification d'acide nucléique
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