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WO1991014706A3 - Lectin affinity membranes and methods for using them - Google Patents

Lectin affinity membranes and methods for using them Download PDF

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Publication number
WO1991014706A3
WO1991014706A3 PCT/US1991/001578 US9101578W WO9114706A3 WO 1991014706 A3 WO1991014706 A3 WO 1991014706A3 US 9101578 W US9101578 W US 9101578W WO 9114706 A3 WO9114706 A3 WO 9114706A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
glycosylated proteins
affinity
glycosylated
lectin affinity
Prior art date
Application number
PCT/US1991/001578
Other languages
French (fr)
Other versions
WO1991014706A2 (en
Inventor
Oscar Dile Holton
Yves Fouron
Original Assignee
Sepracor Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sepracor Inc filed Critical Sepracor Inc
Publication of WO1991014706A2 publication Critical patent/WO1991014706A2/en
Publication of WO1991014706A3 publication Critical patent/WO1991014706A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D67/00—Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
    • B01D67/0081—After-treatment of organic or inorganic membranes
    • B01D67/0093—Chemical modification
    • B01D67/00931—Chemical modification by introduction of specific groups after membrane formation, e.g. by grafting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28014—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their form
    • B01J20/28033—Membrane, sheet, cloth, pad, lamellar or mat
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Inorganic Chemistry (AREA)
  • Transplantation (AREA)
  • Manufacturing & Machinery (AREA)
  • Analytical Chemistry (AREA)
  • Water Supply & Treatment (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)

Abstract

This invention relates to lectin affinity membranes and methods for carrying out affinity separations of glycosylated proteins using them. The method utilizes a membrane-bound lectin ligand first to capture and separate a glycosylated protein from a fluid mixture and subsequently to release said protein in purified form. The present affinity membrane methods recover high-purity glycosylated proteins. The invention has particular relevance to the recovery and purification of glycosylated proteins from mixtures of biological origin.
PCT/US1991/001578 1990-03-19 1991-03-08 Lectin affinity membranes and methods for using them WO1991014706A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US49546890A 1990-03-19 1990-03-19
US495,468 1990-03-19

Publications (2)

Publication Number Publication Date
WO1991014706A2 WO1991014706A2 (en) 1991-10-03
WO1991014706A3 true WO1991014706A3 (en) 1991-11-14

Family

ID=23968753

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1991/001578 WO1991014706A2 (en) 1990-03-19 1991-03-08 Lectin affinity membranes and methods for using them

Country Status (1)

Country Link
WO (1) WO1991014706A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2275270A (en) * 1993-02-11 1994-08-24 Pall Corp Membranes for use in affinity separation
CA2516403C (en) 2003-01-17 2014-08-12 Aethlon Medical, Inc. Method for removal of viruses from blood by lectin affinity hemodialysis
WO2004090549A1 (en) * 2003-04-11 2004-10-21 Novozymes A/S Method of screening for secreted glycosylated polypeptides
DE102005001191A1 (en) * 2005-01-10 2006-07-20 Qiagen Gmbh Apparatus and method for fractionating mono- and / or oligosaccharide structures
US20070037933A1 (en) * 2005-08-11 2007-02-15 Kurth Christopher J Surface modified polymer matrices and methods for their preparation
EP3769083A1 (en) 2018-03-21 2021-01-27 Waters Technologies Corporation Non-antibody high-affinity-based sample preparation, sorbents, devices and methods

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD159569A1 (en) * 1981-06-05 1983-03-16 Manfred Kuehn Test agent and method for the determination of glycosylated proteins
WO1990004609A1 (en) * 1988-10-17 1990-05-03 Sepracor, Inc. Process for the covalent surface modification of hydrophobic polymers and articles made therefrom

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD159569A1 (en) * 1981-06-05 1983-03-16 Manfred Kuehn Test agent and method for the determination of glycosylated proteins
WO1990004609A1 (en) * 1988-10-17 1990-05-03 Sepracor, Inc. Process for the covalent surface modification of hydrophobic polymers and articles made therefrom

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Chemical Abstracts, Volume 110, No. 25, issued 1989, June 19 (Columbus, Ohio, USA),M.G. SCHER et al. "Stabilization of immobilized lectin columns by cross- linking with glutaraldelujde ", see pages 299-300, right and left columns, the abstract-No. 227 913p, Anal. Biochem. 1989,177(1), 168-71. *

Also Published As

Publication number Publication date
WO1991014706A2 (en) 1991-10-03

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