USRE28575E - Indicator for detecting hydrogen peroxide and peroxidative compounds containing alpha naphthoflavone - Google Patents
Indicator for detecting hydrogen peroxide and peroxidative compounds containing alpha naphthoflavone Download PDFInfo
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- USRE28575E USRE28575E US45912674A USRE28575E US RE28575 E USRE28575 E US RE28575E US 45912674 A US45912674 A US 45912674A US RE28575 E USRE28575 E US RE28575E
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
- G01N31/22—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators
- G01N31/228—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators for peroxides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/721—Haemoglobin
- G01N33/725—Haemoglobin using peroxidative activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
Definitions
- ABSTRACT Alpha naphthoflavone is an excellent indicator for detecting hydrogen peroxide and peroxidative compounds such as hemoglobin. When formulated with either a peroxidative active compound or a peroxide, this indicator provides a very sensitive chromogenic response to the presence of said constituents in aque- 7 @Claims, No Drawings It/latter enclosed in heavy brackets appears in the original patent but forms no' part of this reissue specification; matt er printed in italics indicates the additions made by reissue.
- test system may comprise the reagent by dissolving the components in asuitable solvent, impregnating the strips with the resulting solution and drying the impregnated test strips. Details of the compositions contemplated are' set forth in the following examples; I I I I DESCR IPTIONOF THE PREFERRED I EMBODIMENTS.
- Example 1 composition was prepared by mixingv the following "components in thegvolumes indicated below: I Glucose oxidase '5 ml. of an aqueoussolution containing 1000 International units per ml. I I Peroxidasel mlfof an aqueous solution containing 2 mg./ml. i i
- Example-2' Another composition was prepared. employing no peroxidase as shown below: I t Glucose oxidase3 ml. of an aqueous solution containing 1 0( )0 International units/ml. Potassiumiodi de80 mg. I Ammoniummolyb'datel60 mg. 1 Sodium phosphate buffer3 ml. of a l; M
- the strips When dipped into urine containing no glucose, the strips show no color change. The intensity of the blue color is enhanced in solution of the presence of polyvinyl alcohol.
- molybdates and iodides are substituted for the peroxidase, the a-naphthoflavone minimizes the inhibiting effects of acetoacetate which is also an iodine receptor. It was found that when the strips containing the composition of Example 2 were dipped into a 1% aqueous solution composition in the form of a tablet, powder or solution,
- bibulous base materials or carriers such as strips of filter paper of glucose containing 0.1% by weight of acetoacetate they began to change from colorless to blue in 30 seconds and the blue color gradually intensified, whereas strips containing o-tolidine started to show a blue color at 1.5 minutes with very little increase in color intensity even after 5 minutes contact with the same glucose solution.
- Example 3 A first solution was prepared containing 1.5 grams of carrageen, 15 grams of polyvinylpyrrolidone, 15 ml. of ethanol and 192 ml. of water.
- a second solution was prepared containing 9.24 gramsof citric acid, 40.79 grams of sodium citrate and 124.8 ml. of water.
- a third solution was prepared containing 4.5 grams ofa maleic anhydride methylvinylether copolymer, 1.5 grams of sodium lauroyl sarcosinate and 105 ml. of water.
- a fourth solution was prepared containing 0.5 gram of peroxidase and 76 ml. of an aqueous solution of glucose oxidase containing 1,000 international units per ml. of water.
- a composition for detecting H and glucose was thereafter prepared containing 9 ml. of a 0.5% ethanol solution of a-naphthoflavone, 0.73 gram of potassium iodide, 9 ml. of ethanol, 5.5 ml. of water, 34.5 ml. of the first solution above, 20.8 ml. of the second solution above, 17.5 ml. of the third solution above and 7.6 ml. of the fourth' solution previously prepared.
- Bibulous paper strips were dipped in said solution and then dried for 10 minutes at 100 C. These strips readily turned from colorless to blue when contacted with urine containing lucose and the blue color increased with the glucose concentration. Such strips also change from colorless to blue when a drop of bloodcontaining urine and a drop of 3% hydrogen peroxide solution is applied thereto.
- the amount of indicator employed could be varied from 0.05% to 0.30% by weight in such compositions whereas the glucose oxidase concentration could vary from 40 to 300 International units per ml. of peroxidase from 0.0 l to 0.05%, the sodium iodide from 0.25% to 1.5%, the ammonium molybdate from 0.1% to 1.5%, and the polyvinyl alcoho] from 0.2% to 2% by weight of ultimate mix at a pH of from 4 to 7.
- compositions for detecting hydrogen peroxide or peroxidative active compounds utilizing the catalytic oxidation of an indicator system [dyestuff by hydrogen peroxide in the presence of the peroxidative active compound the improvement which comprises the use of a-naphthoflavone in combination with a water soluble iodide salt as the indicator system [dyestuffi] 2.
- composition as claimed in claim 1 in which the peroxidative active compound is selected from the group consisting of peroxidase, hemoglobin and molybdate.
- composition as claimed in claim 3 in which the molybdate is present in about 0.1% to 1.5% by weight of said composition.
- composition as claimed in claim 1 which additionally contains iodide] 7.
- a composition for detecting glucose in aqueous fluids which comprises glucose oxidase, a peroxidative active material, a water soluble iodide salt, and
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Abstract
Alpha naphthoflavone is an excellent indicator for detecting hydrogen peroxide and peroxidative compounds such as hemoglobin. When formulated with either a peroxidative active compound or a peroxide, this indicator provides a very sensitive chromogenic response to the presence of said constituents in aqueous fluids.
Description
United States Patent [191 Bauer 11 E Re. 28,575
[ Reissued Oct. 21, 1975 [54] INDICATOR FOR DETECTING HYDROGEN PEROXIDE AND PEROXIDATIVE COMPOUNDS CONTAINING ALPHA NAPHTHOFLAVONE [75] Inventor: Robert Bauer, Bristol, Ind.
[73] Assignee: Miles Laboratories, Inc., Elkhart,
' Ind.
[22] Filed: Apr. 8, 1974 [21] Appl. No.'. 459,126
Related US. Patent Documents [58] Field of Search 252/408; 8/1 R; 23/230 B, 23/253 TP; 195/1035 C [56] References Cited UNITED STATES PATENTS 2,799,660 7/l957 Nicholls 252/408 3,066,081 11/1962 Rorem 195/1035 X 3,092,465 6/1963 Adams t 23/253 3,183,173 5/1965 Oakes 23/230 3,814,668 6/1974 Blake 23/253 TP X Primary ExaminerBenjamin R. Padgett Assistant Examiner-David Leland Attorney, Agent, or FirmAndrev v L. Klawitter [57] ABSTRACT Alpha naphthoflavone is an excellent indicator for detecting hydrogen peroxide and peroxidative compounds such as hemoglobin. When formulated with either a peroxidative active compound or a peroxide, this indicator provides a very sensitive chromogenic response to the presence of said constituents in aque- 7 @Claims, No Drawings It/latter enclosed in heavy brackets appears in the original patent but forms no' part of this reissue specification; matt er printed in italics indicates the additions made by reissue.
BACKGROUND OF 'THE INYENTION r The determination of glucose in urine isimportant since thistest is employed to detect'diabetes. Procedures for the detection of sugar in urine are well known in "clinical chemistry. One such procedure utilizes Benedicts copper reduction test, another employs a self heating alkaline copper reduction. testin tablet form, while still'another test depends solely on the action of enzymes. The diagnostic composition in most glucose tests comprises essentially glucose oxidase, peroxidase and an indicator which is oxidizedbyhydr'ogen peroxide and undergoes a color reaction during such oxidation. Typical'indicators employed in' the past include o-tolidine, benzidine dianisidine and 27- diamino'fluorene. I a i [t is well known that glucose oxidase'catalyz'es the aerobic oxidation of glucose to gluconic acid and hydrogen peroxide. However .in the presence .of "iodide, H o oxidizes the iodide to free iodine which produces a color change in the indicator. The color change. pro- SUMMARY OF THE INVENTION This invention is predicated upon the discovery that a-naphthoflavone can be used as an indicator dyestuff in formulations containing either peroxide or a peroxidative active compound when the peroxidative active I compound catalyzes the oxidation of the indicator by the peroxide. Said indicator has the formula and is known chemically as 2-phenyl-4H naphtho l ,2-b]pyran-4-one.
Although the test system may comprise the reagent by dissolving the components in asuitable solvent, impregnating the strips with the resulting solution and drying the impregnated test strips. Details of the compositions contemplated are' set forth in the following examples; I I I I DESCR IPTIONOF THE PREFERRED I EMBODIMENTS.
Example 1 composition was prepared by mixingv the following "components in thegvolumes indicated below: I Glucose oxidase '5 ml. of an aqueoussolution containing 1000 International units per ml. I I Peroxidasel mlfof an aqueous solution containing 2 mg./ml. i i
Example-2' Another composition was prepared. employing no peroxidase as shown below: I t Glucose oxidase3 ml. of an aqueous solution containing 1 0( )0 International units/ml. Potassiumiodi de80 mg. I Ammoniummolyb'datel60 mg. 1 Sodium phosphate buffer3 ml. of a l; M
pH6.. v I v a-Naphthoflavonel ml. of a 0.5% ethanol solution. .Water-S ml. I i i Porous paper strips about A. inch wide long were dipped into the above compositions respectively so that about one-half inch of each strip at one end was completely impregnated. The strips were then dried at 100 C. for 10 minutes. If desired, other porous materials such as wood or plastics can be used as carriers. When contacted with urine containing glucose, such test strips give a positive reaction in 1 minute or less as evidenced by the change in color of the indicator from colorless to blue. The formation of color depends upon the liberation of free iodine from the iodide by the action of hydrogen peroxide. When dipped into urine containing no glucose, the strips show no color change. The intensity of the blue color is enhanced in solution of the presence of polyvinyl alcohol. When molybdates and iodides are substituted for the peroxidase, the a-naphthoflavone minimizes the inhibiting effects of acetoacetate which is also an iodine receptor. It was found that when the strips containing the composition of Example 2 were dipped into a 1% aqueous solution composition in the form of a tablet, powder or solution,
it is preferable to affix said composition on bibulous base materials or carriers such as strips of filter paper of glucose containing 0.1% by weight of acetoacetate they began to change from colorless to blue in 30 seconds and the blue color gradually intensified, whereas strips containing o-tolidine started to show a blue color at 1.5 minutes with very little increase in color intensity even after 5 minutes contact with the same glucose solution.
Example 3 A first solution was prepared containing 1.5 grams of carrageen, 15 grams of polyvinylpyrrolidone, 15 ml. of ethanol and 192 ml. of water.
and 3 inches A second solution was prepared containing 9.24 gramsof citric acid, 40.79 grams of sodium citrate and 124.8 ml. of water. y
A third solution was prepared containing 4.5 grams ofa maleic anhydride methylvinylether copolymer, 1.5 grams of sodium lauroyl sarcosinate and 105 ml. of water.
Still, a fourth solution was prepared containing 0.5 gram of peroxidase and 76 ml. of an aqueous solution of glucose oxidase containing 1,000 international units per ml. of water.
A composition for detecting H and glucose was thereafter prepared containing 9 ml. of a 0.5% ethanol solution of a-naphthoflavone, 0.73 gram of potassium iodide, 9 ml. of ethanol, 5.5 ml. of water, 34.5 ml. of the first solution above, 20.8 ml. of the second solution above, 17.5 ml. of the third solution above and 7.6 ml. of the fourth' solution previously prepared. Bibulous paper strips were dipped in said solution and then dried for 10 minutes at 100 C. These strips readily turned from colorless to blue when contacted with urine containing lucose and the blue color increased with the glucose concentration. Such strips also change from colorless to blue when a drop of bloodcontaining urine and a drop of 3% hydrogen peroxide solution is applied thereto.
in addition to the compositions set forth in the foregoing examples, it was found that the amount of indicator employed could be varied from 0.05% to 0.30% by weight in such compositions whereas the glucose oxidase concentration could vary from 40 to 300 International units per ml. of peroxidase from 0.0 l to 0.05%, the sodium iodide from 0.25% to 1.5%, the ammonium molybdate from 0.1% to 1.5%, and the polyvinyl alcoho] from 0.2% to 2% by weight of ultimate mix at a pH of from 4 to 7.
What is claimed is:
1. In a composition for detecting hydrogen peroxide or peroxidative active compounds utilizing the catalytic oxidation of an indicator system [dyestuff by hydrogen peroxide in the presence of the peroxidative active compound, the improvement which comprises the use of a-naphthoflavone in combination with a water soluble iodide salt as the indicator system [dyestuffi] 2. A composition as claimed in claim 1 in which a-naphthoflavone is present in about 0.05% to 0.30% by weight of said composition.
3. A composition as claimed in claim 1 in which the peroxidative active compound is selected from the group consisting of peroxidase, hemoglobin and molybdate.
4. A composition as claimed in claim 3 in which the molybdate is present in about 0.1% to 1.5% by weight of said composition.
5. A composition as claimed in claim 3 in which the peroxidase is present in about 0.01% to 0.05% by weight of said composition.
[6. A composition as claimed in claim 1 which additionally contains iodide] 7. A composition as claimed in claim I [6] in which the water soluble iodide salt is present in about 0.25% to 1.5% by weight of said composition.
8. A composition for detecting glucose in aqueous fluids which comprises glucose oxidase, a peroxidative active material, a water soluble iodide salt, and
a-naphthoflavone.
Claims (7)
1. IN A COMPOSITION FOR DETECTING HYDROGEN PEROXIDE OR PEROXIDATIVE ACTIVE COMPOUNDS UTILIZING THE CATALYTIC OXIDATION OF AN INDICATOR SYSTEM (DYESTUFF) BY HYDROGEN PEROXIDE IN THE PRESENCE OF THE PEROXIDATIVE ACTIVE COMPOUND, THE IMPROVEMENT WHICH COMPRISES THE USE OF A-NAPHTHOFLAVONE IN COMBINATION WITH WATER SOLUBLE IODIDE SALT AS THE INDICATOR SYSTEM (DYESTUFF)
2. A composition as claimed in claim 1 in which Alpha -naphthoflavone is present in about 0.05% to 0.30% by weight of said composition.
3. A composition as claimed in claim 1 in which the peroxidative active compound is selected from the group consisting of peroxidase, hemoglobin and molybdate.
4. A composition as claimed in claim 3 in which the molybdate is present in about 0.1% to 1.5% by weight of said composition.
5. A composition as claimed in claim 3 in which the peroxidase is present in about 0.01% to 0.05% by weight of said composition.
7. A composition as claimed in claim 1 (6) in which the water soluble iodide salt is present in about 0.25% to 1.5% by weight of said composition.
8. A composition for detecting glucose in aqueous fluids which comprises glucose oxidase, a peroxidative active material, a water soluble iodide salt, and Alpha -naphthoflavone.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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US45912674 USRE28575E (en) | 1971-03-01 | 1974-04-08 | Indicator for detecting hydrogen peroxide and peroxidative compounds containing alpha naphthoflavone |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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US11992671A | 1971-03-01 | 1971-03-01 | |
US45912674 USRE28575E (en) | 1971-03-01 | 1974-04-08 | Indicator for detecting hydrogen peroxide and peroxidative compounds containing alpha naphthoflavone |
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USRE28575E true USRE28575E (en) | 1975-10-21 |
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US45912674 Expired USRE28575E (en) | 1971-03-01 | 1974-04-08 | Indicator for detecting hydrogen peroxide and peroxidative compounds containing alpha naphthoflavone |
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Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4956300A (en) | 1982-01-05 | 1990-09-11 | Helena Laboratories Corporation | Aid for determining the presence of occult blood, method of making the aid, and method of using the aid |
US5081040A (en) * | 1987-06-29 | 1992-01-14 | Helena Laboratories Corporation | Composition and kit for testing for occult blood in human and animal excretions, fluids, or tissue matrixes |
US5155024A (en) * | 1986-07-10 | 1992-10-13 | Eastman Kodak Company | Binder composition and analytical element having stabilized peroxidase in layer containing the composition |
US5166054A (en) * | 1991-01-02 | 1992-11-24 | Becton, Dickinson And Company | Method for immunoassay using lactoperoxidase, starch and iodine |
US5196167A (en) * | 1989-04-04 | 1993-03-23 | Helena Laboratories Corporation | Fecal occult blood test product with positive and negative controls |
US5217874A (en) * | 1989-04-04 | 1993-06-08 | Helena Laboratories Corporation | Fecal occult blood test product with positive and negative controls |
US5273888A (en) * | 1984-01-16 | 1993-12-28 | Helena Laboratories Corporation | Chemical test kit and method for determining the presence of blood in a specimen and for verifying the effectiveness of the chemicals |
US5702913A (en) | 1983-12-21 | 1997-12-30 | Helena Laboratories Corporation | Chromgen-reagent test system |
US6635439B2 (en) | 2000-12-21 | 2003-10-21 | Ethicon, Inc. | Hydrogen peroxide indicator employing enzyme and dye |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2799660A (en) * | 1954-04-15 | 1957-07-16 | Miles Lab | Blood test composition and method |
US3066081A (en) * | 1961-05-05 | 1962-11-27 | Edward S Rorem | Means for detecting galactose |
US3092465A (en) * | 1960-03-25 | 1963-06-04 | Miles Lab | Diagnostic test device for blood sugar |
US3183173A (en) * | 1963-06-10 | 1965-05-11 | Miles Lab | Test composition for detecting hydrogen peroxide |
US3814668A (en) * | 1969-10-15 | 1974-06-04 | Miles Lab | Method and device for the semi-quantitative determination of glucose in aqueous fluids |
-
1974
- 1974-04-08 US US45912674 patent/USRE28575E/en not_active Expired
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2799660A (en) * | 1954-04-15 | 1957-07-16 | Miles Lab | Blood test composition and method |
US3092465A (en) * | 1960-03-25 | 1963-06-04 | Miles Lab | Diagnostic test device for blood sugar |
US3066081A (en) * | 1961-05-05 | 1962-11-27 | Edward S Rorem | Means for detecting galactose |
US3183173A (en) * | 1963-06-10 | 1965-05-11 | Miles Lab | Test composition for detecting hydrogen peroxide |
US3814668A (en) * | 1969-10-15 | 1974-06-04 | Miles Lab | Method and device for the semi-quantitative determination of glucose in aqueous fluids |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4956300A (en) | 1982-01-05 | 1990-09-11 | Helena Laboratories Corporation | Aid for determining the presence of occult blood, method of making the aid, and method of using the aid |
US5702913A (en) | 1983-12-21 | 1997-12-30 | Helena Laboratories Corporation | Chromgen-reagent test system |
US5273888A (en) * | 1984-01-16 | 1993-12-28 | Helena Laboratories Corporation | Chemical test kit and method for determining the presence of blood in a specimen and for verifying the effectiveness of the chemicals |
US5155024A (en) * | 1986-07-10 | 1992-10-13 | Eastman Kodak Company | Binder composition and analytical element having stabilized peroxidase in layer containing the composition |
US5081040A (en) * | 1987-06-29 | 1992-01-14 | Helena Laboratories Corporation | Composition and kit for testing for occult blood in human and animal excretions, fluids, or tissue matrixes |
US5196167A (en) * | 1989-04-04 | 1993-03-23 | Helena Laboratories Corporation | Fecal occult blood test product with positive and negative controls |
US5217874A (en) * | 1989-04-04 | 1993-06-08 | Helena Laboratories Corporation | Fecal occult blood test product with positive and negative controls |
US5166054A (en) * | 1991-01-02 | 1992-11-24 | Becton, Dickinson And Company | Method for immunoassay using lactoperoxidase, starch and iodine |
US6635439B2 (en) | 2000-12-21 | 2003-10-21 | Ethicon, Inc. | Hydrogen peroxide indicator employing enzyme and dye |
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