US3595090A

US3595090A - Apparatus for drawing fluid into, and discharging fluid from, a pipette

Info

Publication number: US3595090A
Application number: US858809A
Authority: US
Inventors: Michael E Drummond; John E Robinson
Original assignee: DRUMMOND INSTR CO
Current assignee: DRUMMOND INSTR CO; DRUMMOND INSTRUMENT CO
Priority date: 1969-09-17
Filing date: 1969-09-17
Publication date: 1971-07-27
Anticipated expiration: 1988-07-27

Abstract

An apparatus for drawing fluid into, and discharging fluid from, a pipette. The apparatus includes a tubular member having caps sealing both ends thereof, a pipette partially inserted into the tubular member through one of the caps and a cylindrical member inserted through the other cap partially into the tubular member. A piston is slidably positioned therein and has a plunger manually operated so as to move the piston longitudinally of the cylindrical member. A squeeze bulb is fitted over a terminal of the tubular member, the squeeze bulb having an aperture through which the cylindrical member extends and complete evacuation of the tube is effected by exerting pressure on the squeeze bulb which forces air into the pipette to expel any fluid remaining therein. The outer periphery of the cylindrical member is slightly spaced from the wall of the cap defining the aperture therein to provide communication between the squeeze bulb and the interior of the tubular member.

Description

United States Patent [72] Inventors Michael E. Drummond Springfield; John E. Robinson. West Chester. both of. Pa. [21 1 App]. No. 858,809 [22] Filed Sept. 17,1969 [45] Patented July 27, 1971 [73] Assignee Drummond Instrument Company Broomall, Pa.

{54] APPARATUS FOR DRAWING FLUID INTO, AND DISCHARGING FLUID FROM, A PIPE'I'IE 9 Claims, 4 Drawing Figs.

[52] US. Cl 73/425.6, 73/425.4 P [51] Int. Cl C(lln 1/14 [50] Field of Search 73/4254 P, 425.6; 141/25-27; 222/207, 210

[56] References Cited UNITED STATES PATENTS 2,153,105 4/1939 Szecsi..l 73/425.6 2,218,899 10/1940 Warren 73/4256 2,959,964 11/1960 Streitfeld 73/4256 3,162,217 12/1964 Poli,Jr.etal. 141/27 3,233,785 2/1966 Burke 141/26 (X) 2,561,273 7/1951 Hamiliton 73/4256 FOREIGN PATENTS 914,790 7/1949 Germany; 73/425.6

Primary Examiner-Louis R. Prince Assistant Examiner-Daniel M. Yasich Attorney-Shlesinger, Arkwright and Garvey ABSTRACT: An apparatus for drawing fluid into, and discharging fluid from, a pipette. The apparatus includes a tubular member having caps sealing both ends thereof, a pipette partially inserted into the tubular member through one of the caps and a cylindrical member inserted through the other cap partially into the tubular member. A piston is slidably positioned therein and has a plunger manually operated so as to move the piston longitudinally of the cylindrical member. A squeeze bulb is fitted over a terminal of the tubular member, the squeeze bulb having an aperture through which the cylindrical member extends and complete evacuation of the tube is effected by exerting pressure on the squeeze bulb which forces air into the pipette to expel any fluid remaining therein. The outer periphery of the cylindrical member is slightly spaced from the wall of the cap defining the aperture therein to provide communication between the squeeze bulb and the interior of the tubular member.

PATENTEU JUL27|97l 3, 595,090

INVENTORS Michael E. Drummond BY John E. Robinson ATTORN YS APPARATUS FOR DRAWING FLUID INTO, AND DISCHARGING FLUID FROM, A PIPETTE OBJECTS OF THE INVENTION It is an object of this invention to provide apparatus for drawing fluid into, and discharging fluid from, a pipette by means of a pneumatic control, the pipette and pneumatic control being inserted into opposite ends of a tubular member which is closed at both ends, for selectively and accurately drawing fluid into the pipette, and for discharging the fluid from the pipette when desired.

Another object is to provide apparatus of the character described wherein the pneumatic control includes a piston operable longitudinally of a cylindrical member, one terminal of which is partially inserted into the tubular member, means being connected to the piston to effect manual movement of the piston within the cylindrical member to accurately draw fluid into the pipette, by movement of the piston in one direction, and to discharge the fluid from the pipette by movement of the piston in the opposite direction.

A further object is to provide apparatus as described, with the addition of a squeeze bulb connected to the apparatus for effecting complete evacuation of the fluid from the pipette.

A still further object is to provide apparatus of the character described including a plunger which terminates in a finger loop for ready operation of the piston within the cylindrical member by the user.

Other objects will be manifest from the following description of the presently preferred form of the present invention taken in conjunction with the appended drawings.

DESCRIPTION OF FIGURES OF THE DRAWING FIG. 1 is a side elevational view of the apparatus of the present invention;

FIG. 2 is an enlarged longitudinal sectional view of the same;

FIG. 3 is a sectional view taken along the line 3-3 of FIG. 2, looking in the direction of the arrows, and

FIG. 4 is a view similar to FIG. 1 illustrating the operation of the squeeze bulb forming a part of the present invention.

DETAILED DESCRIPTION OF THE INVENTION The apparatus of the present invention includes a tubular member which is open at both ends. A removable cap 12 of rubber or plastic is inserted into one end of tubular member 10, the outer periphery of one end of the cap being enlarged at 14 to limit the entry of the cap into the tubular member. Cap 12 is provided with a central aperture 16 which as shown to advantage in FIG. 2 is conical in conformation to facilitate insertion of one terminal of pipette 18 partially into tubular member 10.

The'opposite terminal of tubular member 10 as provided with a removable cap 20 constructed of Teflon or other suitable rigid material, the main body portion thereof being in substantially airtight relationship with the inner periphery of tubular member 10. The outer extremity of cap 20 is enlarged to provide an abutment flange 22 engageable with the near terminal of tubular member 10.

It is a salient feature of the present invention to provide a pneumatic assembly generally designated 24 which is operable within tubular member 10. Assembly 24 includes a cylindrical member 26 which is inserted through an axial opening in cap 20, the cross-sectional area of the opening being larger then that of the cylindrical member, thereby providing a space 28 between the cylindrical member and the cap for purposes which will be hereinafter more fully set out.

A piston 30 made of Teflon or other suitable material is positioned within, and engageable with the walls of, cylindrical member 26 for longitudinal movement therein. A plunger or rod 32, made of a suitable material, such as stainless steel, is attached to one end of piston 30, which plunger extends completely through cylindrical member 26 and terminates in a finger loop 34, which facilitates operation by the user.

The pneumatic assembly further includes a squeeze bulb 36 of elongated resilient construction, one terminal of which 38 is domed-shaped and provided with an aperture 40 through which cylindrical member 26 passes. It will be noted that aperture 40 is substantially the same size as the cylindrical member in order to provide an airtight relationship between these members.

The opposite tenninal of squeeze bulb 36 is flanged at 42 for sleeved engagement over abutment flange 22 of cap 20 and the adjacent terminal of tubular member 10. There is thereby provided an air space 44 between the walls of squeeze bulb 36 and cylindrical member 26.

Referring now to FIG. 2, it will be noted that when the outer terminal of pipette 18 is placed in a fluid, a closed air chamber is created within space 46, between

caps

12 and 20, with the result that any movement of piston 30 longitudinally of cylindrical member 28 will affect the air pressure within pipette l8.

OPERATION In use of the apparatus of the present invention, the tip, of pipette I8 is inserted in the fluid to be drawn into the pipette, at which time plunger 32 is in the lowered position shown in dotted lines in FIG. 1. Upon finger engagement with loop 34 and movement of plunger 32 outwardly, by virtue of the closed air chamber within tubular member 10, a suctioning pressure is exerted within pipette 18 to draw the fluid into the pipette, the amount and rate of flow of the fluid drawn into the pipette being accurately controlled by the movement of piston 30 within cylindrical'member 28.

When the desired amount of fluid has been drawn into pipette 18, it is removed from the fluid, and piston 30 remains in the same position within cylindrical member 28 to retain the fluid within the pipette.

The fluid is dispensed by pushing plunger 32 downwardly with loop 34 to move piston 30 towards the inner end of cylindrical member 28, thereby increasing the air pressure within the adjacent end of terminal 18, and discharging the fluid from the pipette.

In order to discharge any remaining drops of fluid which may be retained in the pipette, finger pressure is inserted on squeeze bulb 36 as shown to advantage in FIG. 4, thereby causing the air within space 44 to travel through space 28 into closed air chamber 46 of tubular member 10. This exerts additional air pressure through pipette 18 in a direction to discharge any drops of fluid within pipette 1%.

Alternatively, the fluid within the pipette may be quickly and completely expelled from the pipette by squeezing bulb 36.

It has been found that optimum results are obtained when the total internal volume of the apparatus, including the tubular member and the squeeze bulb is approximately 1500 microliters and the volume of the small cylindrical member is in the range of I00 to 300 microliters. The ratio of apparatus volume to cylindrical member is, therefore, in the range of I5zl to 5:1.

It has further been determined that a small cylindrical member volume of 150 microliters should be employed when using pipettes in the size range of 20 to microliters and that a 250 microliter volume cylindrical member should be employed for pipettes in the size range of 50 to 200 microliters.

What we claim is:

1. Apparatus for drawing fluid into, and discharging fluid from, a pipette including:

a. a transparent tubular member,

b. caps sealing both ends of said tubular member,

c. one of said caps having an aperture in communication with said tubular member,

(I. a pipette partially inserted through the aperture of said one cap into said tubular member,

e. a cylindrical member partially inserted into said tubular member through said other cap,

. said cylindrical member being spaced from said tubular member,

. a piston within, and movable longitudinally through, said cylindrical member,

h. a plunger one terminal of which is connected to said piston and extending through said cylindrical member,

. the free terminal of said plunger issuing into a finger engaging loop,

j. whereby upon placing the outer end of the pipette into a fluid and moving said piston outwardly through said cylindrical member a controlled quantity of fluid is drawn into the pipette and, upon inward movement of the piston, the fluid is expelled from the pipette, and

. a resilient squeeze bulb,

. one terminal of said squeeze bulb being dome shaped and provided with an aperture through which said cylindrical member passes,

in. the outer terminal of said squeeze bulb being engaged over a terminal of said tubular member,

n. the outer periphery of said cylindrical member being slightly spaced from the wall of said other cap defining the aperture therein,

0. whereby upon squeezing of said squeeze bulb air is directed under pressure through the space between the cylindrical member and the other cap aperture, and through the tubular member into the inner end of the pipette for exerting an expelling force on fluid within the pipette.

. The apparatus of claim 1 wherein:

. the ratio of the volume of the apparatus including the tubular member and squeeze bulb with respect to the volume of said cylindrical member, is in the range of :1 to 5:1.

. The apparatus of claim 2, wherein:

. a cylindrical member having a volume of 250 microliters is employed for pipettes in the size range of 50 to 200 microliters.

. The apparatus of claim 2, wherein:

. the volume of the tubular member and squeeze bulb is approximately l5OO microliters, and the volume of the small cylindrical member is in the range of 100 to 300 microliters.

. The apparatus of claim 4, wherein:

. a cylindrical member having a volume of 150 microliters is employed for pipettes in the size range of to 100 microliters.

6. Apparatus for drawing fluid into, and discharging fluid from, a pipette including:

a. a tubular member,

b. caps sealing both ends of said tubular member,

c. both of said caps being provided with apertures,

d. a pipette inserted through the aperture of one of the said caps partially into said tubular member,

e. a cylindrical member inserted through the aperture of said other cap partially into said tubular member,

f. a piston slidably positioned in said cylindrical member,

g. means connected to said piston for moving the latter within said cylindrical member,

h. whereby upon placing the outer end of the pipette into a fluid and moving said piston outwardly through said cylindrical member a controlled quantity of'fluid is drawn into the pipette and, upon inward movement of the piston, is expelled from the pipette, and

i. a resilient squeeze bulb engaged over a terminal of said tubular member,

j. said squeeze bulb being provided with an aperture through which said cylindrical member extends to a point beyond the squeeze bulb,

. the outer periphery of said cylindrical member being slightly spaced from the wall of said other cap defining the aperture therein, 1. whereby, upon squeezing of said squeeze bulb, air is directed under pressure through the space between the cylindrical member and the other cap aperture, and through the tubular member into the inner end of the pipette, for exerting an expelling force on the fluid within the pipette.

7. The apparatus of claim 6, wherein:

a. the ratio of the volume of the apparatus including the tubular member and squeeze bulb with respect to the volume of said cylindrical member, is in the range of 15:1 to 5: l.

8. The apparatus of claim 6, wherein:

a, the volume of the tubular member and squeeze bulb is approximately 1500 microliters, and the volume of the small cylindrical member is in the range of to 300 microliters.

9. Apparatus for drawing fluid into, and discharging fluid from, a pipette, including a. a large tubular member,

b. cap means sealing both ends of said tubular member,

forming an air chamber therebetween,

said cap means being provided with apertures,

. a pipette inserted through the aperture of one of said cap means partially into the air chamber of said tubular member,

. a small cylindrical member inserted through the aperture of said other cap means partially into the air chamber of said other tubular member,

a piston within, and movable longitudinally through. said small cylindrical member,

a plunger, one terminal of which is connected to said piston, for moving the piston through the small cylindrical member,

. a collapsible bulb mounted over one end of said large tubular member, providing a closed chamber, and

. an air passageway in said other cap means connecting the closed chamber of said collapsible bulb with the air chamber of said large tubular member,

jv whereby, upon squeezing of said collapsible bulb, air is directed under pressure through the air passageway, and through the tubular member into the inner end of the pipette, for exerting and expelling force on the fluid within the pipette.

Claims

1. Apparatus for drawing fluid into, and discharging fluid from, a pipette including: a. a transparent tubular member, b. caps sealing both ends of said tubular member, c. one of said caps having an aperture in communication with said tubular member, d. a pipette partially inserted through the aperture of said one cap into said tubular member, e. a cylindrical member partially inserted into said tubular member through said other cap, f. said cylindrical member being spaced from said tubular member, g. a piston within, and movable longitudinally through, said cylindrical member, h. a plunger one terminal of which is connected to said piston and extending through said cylindrical member, i. the free terminal of said plunger issuing into a finger engaging loop, j. whereby upon placing the outer end of the pipette into a fluid and moving said piston outwardly through said cylindrical member a controlled quantity of fluid is drawn into the pipette and, upon inward movement of the piston, the fluid is expelled from the pipette, and k. a resilient squeeze bulb, l. one terminal of said squeeze bulb being dome shaped and provided with an aperture through which said cylindrical member passes, m. the outer terminal of said squeeze bulb being engaged over a terminal of said tubular member, n. the outer periphery of said cylindrical member being slightly spaced from the wall of said other cap defining the aperture therein, o. whereby upon squeezing of said squeeze bulb air is directed under pressure through the space between the cylindrical member and the other cap aperture, and through the tubular member into the inner end of the pipette for exerting an expelling force on fluid within the pipette.

2. The apparatus of claim 1 wherein: a. the ratio of the volume of the apparatus including the tubular member and squeeze bulb with respect to the volume of said cylindrical member, is in the range of 15:1 to 5:1.

3. The apparatus of claim 2, wherein: a. a cylindrical member having a volume of 250 microliters is employed for pipettes in the size range of 50 to 200 microliters.

4. The apparatus of claim 2, wherein: a. the volume of the tubular member and squeeze bulb is approximately 1500 microliters, and the volume of the small cylindrical member is in the range of 100 to 300 microliters.

5. The apparatus of claim 4, wherein: a. a cylindrical member having a volume of 150 microliters is employed for pipettes in the size range of 20 to 100 microliters.

6. Apparatus for drawing fluid into, and discharging fluid from, a pipette including: a. a tubular member, b. caps sealing both ends of said tubular member, c. both of said caps being provided with apertures, d. a pipette inserted through the aperture of one of the said caps partially into said tubular member, e. a cylindrical member inserted through the aperture of said other cap partially into said tubular member, f. a piston slidably positioned in said cylindrical member, g. means connected to said piston for moving the latter within said cylindrical member, h. whereby upon placing the outer end of the pipette into a fluid and moving said piston outwardly through said cylindrical member a controlled quantity of fluid is drawn into the pipette and, upon inward movement of the piston, is expelled from the pipette, and i. a resilient squeeze bulb engaged over a terminal of said tubular member, j. said squeeze bulb being provided with an aperture through which said cylindrical member extends to a point beyond the squeeze bulb, k. the outer periphery of said cylindrical member being slightly spaced from the wall of said other cap defining the aperture therein, l. whereby, upon squeezing of said squeeze bulb, air is directed under pressure through the space between the cylindrical member and the other cap aperture, and through the tubular member into the inner end of the pipette, for exerting an expelling force on the fluid within the pipette.

7. The apparatus of claim 6, wherein: a. the ratio of the volume of the apparatus including the tubular member and squeeze bulb with respect to the volume of said cylindrical member, is in the range of 15:1 to 5:1.

8. The apparatus of claim 6, wherein: a. the volume of the tubular member and squeeze bulb is approximately 1500 microliters, and the volume of the small cylindrical member is in the range of 100 to 300 microliters.

9. Apparatus for drawing fluid into, and discharging fluid from, a pipette, including a. a large tubular member, b. cap means sealing both ends of said tubular member, forming an air chamber therebetween, c. said cap means being provided with apertures, d. a pipette inserted through the aperture of one of said cap means partially into the air chamber of said tubular member, e. a small cylindrical member inserted through the aperture of said other cap means partially into the air chamber of said other tubular member, f. a piston within, and movable longitudinally through, said small cylindrical member, g. a plunger, one terminal of which is connected to said piston, for moving the piston through the small cylindrical member, h. a collapsible bulb mounted over one end of said large tubular member, providing a closed chamber, and i. an air passageway in said other cap means connecting the closed chamber of said collapsible bulb with the air chamber of said large tubular member, j. whereby, upon squeezing of said collapsible bulb, air is directed under pressure through the air passageway, and through the tubular member into the inner end of the pipette, for exerting and expelling force on the fluid within the pipette.