US2567651A - J-dialkyl-g-amino-l - Google Patents

Description

Patented Sept. 11, 1951 1,3-DIALKYL-6-AMINO- 1,2,3,4 -TETRA HY- DRO-2,4-PYRI'MIDINEDIONES Viktor Papesch, Morton Grove, and Elmer E, Schroeder, Chicago, Ill., assignors to G. I). Searle & 00., Chicago, Ill., v a corporation of Illinois No Dra ng. Application. January 11, 1950, Serial No. 138,074;

11 Claims. 1

Thepresent invention is concerned generally with substitute diketones of heterocyclic compounds, and more particularly with substitution products of fi-amino-1,2,3,4-tetrahydro-2,4-pyrimidinedione, and the production thereof. The compounds which comprise our invention have the following general structural formula WhereinthomdicahR and R represent a mem- -member of the same class, methyl or ethyl. The

R and R groups maybe different or, in certain instances, identical.

The aminotetrahydropyrimidinediones of this invention have been found to possess useful therapeutic properties, particularly as cardiovascular, diuretic and chemotherapeutic agents.

The prior art discloses several 1,3-dialkyl-6- amino l,2,3,4 tetrahydro 2,4 pyrimidinediones, in which the alkyl group represents methyl and ethyl radicals. However, these known compounds have not been shown to be therapeutically useful and experiments on the diuretic effects of these known compounds in dogs have shown them to be ineiiicacious, whereas the pyrimidinedio-nes of this invention, due to the presence of a larger alkyl or aralkyl group, have shown an effectiveness of a much higher order, some of which are comparable even to the highly potent mercurials. The unusual usefulness of our compounds in comparison to the mercurials is based on two factors.v The pyrimidinediones of our invention may be administered orally, while the mercurials must be injected. Further, while the usefulness of the mercurials is greatly limited by their toxicity, our compounds show a very low order of toxicity. These results obtained in diuretic tests in mammals have also been confirmed; in the clinic.

The following examples illustrate methods by which these aminotetrahydropyrimidinediones may be prepared; One of our preferred methods for obtaining certain 1,3-dialkyl-6-amino-1,2,3,4- tetrahydro-2,4 -pyrimidinediones begins with the treatment of 1,3 -dialkyl derivatives of urea with acetic anhydride and cyanoacetic acid for 30-200 minutes at 50-100 C. We have discovered that in the case of unsymmetrically substituted 1,3- dialkyl ureas the addition of the cyanoacetyl group Occurs primarily at the nitrogen atom to which the smaller alkyl group lsattached. Thus, if R is a radical containing more carbon atoms than R, the addition; occurs primarily as follows;

If the B and R groups are Very similar, a mixture of isomers will be formed. To eifect the cyclization, of; the cyanoacetylurea derivatives we add sufficient alkali tobring; the pH to 7 or higher and maintain the temperature, at. 40 to C. for afewminutes.

In order to prepare the substituted G-aminol,;2 3,4 tetra-hydro 2,4,. pyrimidinedione derivatives substituted in the 3-position by a larger group than the substituent in the 1-position we prefer the following procedure. A monoalkyl urea is treated with acetic anhydride and cyanoacetic acid, with or without acetic acid, for 20 to 200 minutes at,50.100 C. to form the N-a1ky1-N- cyanoacetylurea. For the cyclization it is detrimental to, the yield to use an excess of alkali.

Instead, We prefer to use dilute alkali to make the solution barely alkaline. The reaction is thus completed in only a few minutes. The resultant 1 alkyl 6 amino 1,2,33 tetrahydro 2,4- .pyrimidinediones. are treated with slightly more alkali than necessary to form the alkali metal salt and then with an organic halide or sulfate. An organic solvent may be necessary to keep the alkylating agent in solution. We heat for 1-6 hours at 50-100 C. to efiect the substitution.

The following examples illustrate in detaileertain of the compounds which comprise this invention, and methods of producing them. The invention is not to be construed as limited in spirit or in scope thereby. It will be apparent to those skilled in the art that many modifications in materials and methods may be made without departing from our invention.

EXAMPLE 1 1-n-propyl-6-amino-1,2,3,4-tetrahydro- 2,4-pyrimidinedione 173 g.- of propyllurea are dissolved in 302 ml. of acetic anhydride at'70 C. Then 167 g. cyanoacetic acid are added and the solution is kept at 60 C. until crystallization begins, which usually takes about 1.5 to 2 hours. One liter of ether is added with stirring. After cooling one filters and rinses with ether. To the resultant 160 g. of N-propyl-N-cya.noacetylurea one adds 135 ml. of 10% sodium hydroxide, stirs and heats to 85 C. for 15 minutes. After cooling the pH is adjusted to 5. The white precipitate is filtered and washed with water. 90 g: of the'crude l-n-propyl 6 amino-l,2,3,4-tetrahydro-2,4- pyrimidinedione is obtained. Recrystallization from 40% ethanol gives white crystals which melt at 273-275 C.

EXANHDLEZQ 1-n-propyl-3-methyl-6-amino- 1,2,3,4-tetrahydm-ZA-pm imidinedione 65 g. of l-n-propyl-S-amino-1,2.3,4tetrahy- .dro-2,4-pyrimidinedione are dissolved in 130 ml.

Operating at 60-70 of 10% sodium hydroxide. C. with stirring one adds dropwise first 40 m1. of dimethyl sulfate, then 18 ml. of10% sodium hydroxide and thenagain, dropwise, 10 ml.- of additional dimethyl sulfate. Finally one neutralizes with 10% sodium hydroxide. On cooling, filtration and washing. with water one obtains white crystals which, on recrystallization stirring, a'solution of 54.5 g. of n-propylamine in 200ml. of benzene, the'temperature of the reaction being held at about15 to 25 C. After completion of the addition, the solvent is' removed by vacuum distillation. The residual syrup crystallizes on cooling to give 114 g. of

-Npropyl-N-'ethylurea in practically. pure form.

On recrystallization from '10 parts of petroleum ether, needles melting at 79-80 C. are obtained.

A mixture of 97 g; of the crude N-propyl-N- ethylurea, 76.5 'g. of cyanoacetic acid and 190 ml. of acetic anhydride is heated at 70-80 C. The heat of reaction at the start may necessitate removal '0f the container from the bath until the main reactioniscompleted. The solvent is removed'as completely as possible by vacuum distillation at 70-80? C. "added to the syrupand the solvent again dis- 150 ml. of water are tilled off as completely as possible. 201 g. of a syrup, consisting largely of N-n-propyl-N'-ethyl- N'-cyanoacetylurea is obtained.

100 g. of this crude syrup 'are stirred with 150 ml. of water at 20 C.,and treated with 25 ml.

of a 70% (by weight) aqueous solution of sodium hydroxide. The syrup dissolves completely,but almost immediately, a second, oily product is deposited. The temperature rises to 7,0-75 -C., then drops again. On cooling and stirring,

the oil rapidly crystallizes. Filtration, washing with water and air drying gives 66 g. of crude product.- Two recrystallizations from hot ethyl acetate and air drying yield 47 g. of l-propyl- 3-ethyl-6- amino-123,4 -tetrahydro-2,4-pyrimi- 270 ml. of a cooled 70% sodium hydroxide solution are added to a solution of 300 g. of npropylamine in, 650 ml. of ice water in a 5-liter flask with mechanical stirring. A solution of 263 g. of phosgene in 1320 ml. of benzene, prepared at C., is added to the reaction mixture with cooling at 0-5 C. in the course of two hours. Toward the end of the reaction, the temperature is permitted to rise to about 11 C. Stirring is continued for a half hour. The aqueous layer is separated and washed with benzene,

the benzene solutions are united and dried over.

anhydrous sodium sulfate. The benzene is then distilled off.

372 g. of the resultant di-n-prop-ylurea are dissolved in 335 ml. of glacial acetic acid and 745 ml. of acetic anhydride, Then 238 g. of cyanoacetic acid are added and the mixture kept at -65 C. for 2 hours. Most of the solvent is then distilled off at 80 C. and 10 mm. pressure. After addition of 200 ml. of water, the distillation is resumed. 550 ml. of a 10% sodium hydroxide solution are added to the residue with stirring and the pH adjusted to fairly strong alkalinity to phenolphthalein. The solution becomes hot and an oil is formed which on stirring and cooling crystallizes. The resultant monohydrate of 1,3-di-n-propyl-6-amino-1,23,4- tetrahydro-2,4-pyrimidinedione is recrystallized from 20% ethanol. The white crystals soften at 94 C. and melt at 98100 C. By drying at 80 C. for 8 hours anhydrous crystals are obtained which melt at 136-138.5 C.

EXAMPLE 5 1-isopropyZ-3-methyl-6-amino-1,2,3,4-tetrahydro-2,4-pyrimidincdione A mixture of 25 g. of N-isopropyl-N-methylurea, 22 g. of cyanoacetic acid and 50 ml. of acetic anhydride is heated for 2 hours at 80 C. in a water bath. The solvent is removed as completely as possible by vacuum distillation. 25 ml. of water is added to the residue and vacuum distillation completed. The crude syrup, consisting largely of N-isopropyl-N'-methyl-N'- cyanoacetylurea weighs 48 g.

27 g. of this syrup are treated with 20 m1. of 35% aqueous sodium hydroxide solution. The syrup completely dissolves and the tempera ture rises rapidly to about C. Vigorous stirring is maintained for a few minutes until the temperature begins to drop. An oily product separates which, on further cooling and stirring,

\ solidifies. Filtration, washing; wltlrwaterrand; air

drying yield the.- crude. 1-isopropy1-3-methyl1-damino- 1,2,3,4 tetrahydro 2,4. pyrimidinedir one. After drying for l2;hours. at 80-'C.,,ame1ting point of 210-212 is obtained.

EXAMPLE 6 i isopropyls3-ethyl-6 amino l,2,3,4-tetrahydr0-2,4-pyrimidinedione From '79 g. of ethyl isocyanate in 300 ml. of benzene and 82 g. of isopropylamine in 200 ml. of benzene one obtains, by using the procedure of Example 3, l3 4 g. of; N-isopropyl-N ethylurea, which, on recrystallization from ethyl acetate. melts .at.1'5.8.-15.9.: C. 91 g. of the crude product is reacted with. '71 g, of cyanoacetio. acid and, 175 ml. at; acetic anhydrid as in Example 3 to obtain. 1.60 g. of a. syrup, consisting largely of, N isopropyl N"--,ethylr-N'-cyanoacetylurea. 10.0 g. of this syrupin 150ml. oLwater are then reacted with 3.0 ml. of .-a.70i% aqueous solution of sodium hydroxide. Proceeding as in Example 3 one obtains 64 g. of crudel-isopropykB-ethyl- 6 amino 1,2;3,4. tetrahydro-Z,4-pyrimidinedione, which on two recrystallizations from methanol and drying; at 80 C; furl-2 hours gives 27- g. of cubical prisms melting. at 200-201 C... containing no water of crystallization.

EXAMPLE" 7 1-n-butyl 6-amino-1,2,34-tetrahydro-LL pyrimidinedione .525 g. ofjn-butylurea are dissolved'in 800 mLof acetic anhydride with warming; 440 g. of cyanoe acetic acid are added, and after 2 hours of heating at 60 C. one cools, adds 2 liters of ether. stirs, cools again, filters oil" the white precipitate and rinses with ether. To the resultant 546 g. of N-butyl-N'-eyanoacetylurea one adds 930 ml. of' concentrated aqueous ammonia, dilutes with water to make a volume of 3000 ml., and keeps theqmixture at 70 C. for 30 minutes. The cooled solution is acidified, filtered and the white crystals are washed with water, The yield is 170 g. of 1 n.-;butyl -.6 amino 1,2;3,4 tetrahydro-2,4:-pyrimidinedione, which on recrystallization from dilute alcohol melts at 266- 267 C.

EXAMPLE 8 1-n-butyl-3-methyl-6-amino-1,2,3,4-tetrahydro-2,4-pyrimidinedione 250 g. of 1 n butyl 6 amino-1,23,4- tetrahydro-2,4-pyrimidinedione are dissolved in 500 m1. of sodium hydroxide and 125 ml. of ethanol. Operating as in Example 2 one treats first with 180. ml. of dimethyl sulfate, then with 62.5 ml. of sodium. hydroxide and. finall with m1. of dimethyl sulfate. One obtains 165 g.

of the white monohydrate which, after drying,

meltsat l3,6l38'.

. EXAMPLE 9 1-n butyl-3.-ethyl-6-amino-1,2,3Aetetmhyrho-2,4 -pyrimidinedione crude I n. butyl- 3; ethyl; 6: amino 1.2.3.4-

:tetrahydro..- 2,4 pyrimidinedione are obtained.

The latter is twice recrystallized by dissolving in 5 parts of ethyl acetate and. adding 4 parts of petroleum ether. 24' g. of'the monohydrate is thus obtained as a colorless sol-idv of indefinite crystalline form, melting 'at -72" C.

I EXAMPLE 10 1 ,3-di-1c-butyZ-6-amino-1,2,3,4rtetrahydro- 2,4-1Jyrimidinedione 344g. of di-n-butylurea. are dissolved, in 700 ml. ofuglacial acetic acid and 700 ml. of acetic anhydride, and: 224 g'. of cyanoaceticacid are added. Operating as. in. Example 4 one obtains 388 g. of. hydrated white crystals, melting at 100-104 C.

EXAMPLE 11 1-isobutyl-6-amino-1,2;3,4-tetrrliydro-2,4;- purimidinedione 275 g. of isobutylurea are dissolved in 600ml. acetic anhydride at C. 275 g. of cyanoacetic acid are added, and after 1.5 hours one cools, adds 15 liters of ether, cools again, filters and rinses with ether. To the resultant 255 g. of N- isobutyl-N'-cyanoacetylurea one adds 920 ml. of boiling water and adds at C. and with stirring 175 ml. of 10% sodium hydroxide. The solution is brought. to boiling, allowed to cool, acidified to a pH of 5, cooled again, filtered, and, the white crystalsare washed with water. The yield isl88 g.; the melting point from dilute alcoholis 2'71-'-273 C.

12 1-isobutyl-3-meth.yZ 6 ami1w|-.1,2,3,4-tetrahydro- 2,4-pyrimnldinedionc 100. g. of l- -isobutyl-fi-amino-1,2,3,4-tetrahydro-2,4-pyrimidinedione are dissolved in 200 ml. of 10% sodium hydroxide at 50 C. and, operating as in Example 2,, one treats. with 665 ml. dimethyl sulfate, then with ml. 10% sodium hydroxide and finally with 5 ml. dimethyl sulfate. The solution is stirred for an additional half. hour, neutralized with 10% sodiumhydroxide, cooled, filtered and the precipitate washed and recrystallized with charcoal and a filter aid from 1 liter, of water; of melting point. C- are obtained. Drying for 24 hours at370 C. yields the anhydrous product, which melts at 173-175 C.

EXAMPLE 1'3 I-hemyl-3'-ethyl-(i-aminJo-I,2,3,4-tetra.hyd.ro-2,4-

pyrimidinedione To an. ice-cooled solution of 71 g. of, ethyl isocyanate in 300 ml., of benzene is added, with stirring,a solution of'90 g. of hexylamine in 200 ml. ofbenzene. Onecools to control the reaction and, uponits completion, evaporates the solvent by vacuum distillation.

1.23. g. of N-n-hexyl.-N-ethylurea, 250 ml. of aceticanhydride, 8,0. g. of glacial. acetic acid. and 80 g. of cyanoacetic acid are heated for 2 hours at 55-65 C. Vacuum distillation removes most of the solvent. One then adds ml. of water and resumes the vacuum distillation until almost all of the solvent has disappeared. Enough 10% sodium hydroxide is. added to make the solution alkaline to. phenolphthalein. One warms. to 70 C., and the temperature is maintained for 5 minutes at Z0-80 C- Upon cooling a waxy material. isobtained, which on washing with: water and repeated recrystallization. from ethanol 80 g. of the monohydrate' "melt at 161163 C.

minutes.

yields 1 -hexyl-3-ethyl-6-amino-1;2,3 ,4-tetrahydro 2,4-pyrimidinedione. The' white crystals EXAMPLE14 1 -cyclohemyl-a'-ethyl6-amin0-1,2,3,4- tetrohydTo-ZA-p TimidinediOne To an ice-cooled solutionjof 142 g. of ethyl isocyanate in 400 ml. of benzene is gradually added, with stirring, a solution .of 188 g. of cyclohexylamine in 300 ml. of benzene, the temperature'being held below 30 C. Partial crystallization occurs during the reaction. The mixture is transferred to a large dish and the solvent evapacetic anhydride is heated for 3 hours on awater bath held at about 90 C; The solventis re.- moved as far as possible by vacuum distillation, the syrup poured into a beaker, covered with 200 ml. of water and stirred. The syrupcrystallizes rapidly, the crystals are filtered, washedgwith water and recrystallized from 8 parts of 50% aqueous ethanol, using charcoal to decolorize.

A second recrystallization from the same solvent .gives 300 g. of pure N-cyclohexyl-N'-ethyl-N'- cyanoacetylurea, as rectangular plates, melting at l10-112 C. A mixed melting point with the starting material, N-cyclohexyl-N'-ethylurea, gives a depressed melting point of about 90-95 C., showing the non-identity of the two products. 100 g. of this crystalline N-cyclohexyl-N- ethyl-N-cyanoacetylurea in 150 ml. of water are reacted with 25 ml. of a 70%aqueous solution of sodium hydroxide. Proceeding 'as inEx ample 3, a 30% yield of 1-cyclohexyl-3-ethyl-6- amino-1,2,3,4-tetrahydro-2,4 pyrimidinedione is obtained.

. 1 EXAMPLE I-benzyl-fi-amino-l,'2,3,4-tetrahyda*o-2,4-

pyrimidinedione I e 40 g. of benzylurea are dissolved in 70 mlfof glacial acetic acid and 70 ml. of acetic anhydride.

27 g. of cyanoacetic acid are added and the mixture is heated for 2 hours at 70 C. While the solvent is distilled off under vacuum, precipitation begins. After filtration, the crude N-benzyl- N-cyanoacetylurea is ground with water, stirred and heated with 25 ml. of- 10% sodium hydroxide solution. Enough 70% sodium hydroxide is added to neutralize. The mixture is then boiled and stirred, 10ml. of 10% sodium hydroxide solution is added and boilingcontinued for. 10 After neutralizing with acetic acid and cooling one filtersand washes with water. Upon recrystallization from 3200 ml. of ethanol, 16 g. of pure l-benzyl-S-amino-1,2,3,4-tetrahydro-2,4-pyrimidinedione is obtained which melts at 285-286 C.

EXAMPLE 16 1,3-dibenzyl-fi-ammo-i,2,3,4 -tetmitydro-2,4-

pyrimidinedione 290 g. of dibenzylurea are dissolved in 350 ml. glacial acetic acid and 350 m1. of achtic'anhy dride. 120 g. cyanoacetic'acid are added and the mixture kept at (SO- C. for 2 hours. The solvent is evaporated in vacuum on the steam bath. After removal of about 500 m1. of solve'nt,"the

dione, precipitates.

.pyrimidinedione derivative may precipitate.

4 bath;

'crystallizationstarts. One half volume of'95% ethanol'and one half volume of 10% sodium hy-g droxide are added. The solution is then made alkaline to' phenolphthalein by adding 70%- sodium hydroxide solution. On stirring, 350 g. of the crude monohydrate of 1,3-dibenzyl-6- amino 1,2,3,4 tetrahydro 2,4 pyrimindine- Recrystallization from 60% ethanol yields white crystals which melt at -125. C.

EXAMPLE 1'7 1- ('y phenylpropyl) -3-ethyl-6-umin0-12,3,4-

tetrahydro-2,4-pyrimidinedione To 121 g. of ethyl isocyanate in 300 ml. of benzene are added, with cooling, 217 g. of phenylpropylamine in 200 ml. benzene. Proceeding as in Example 3, the N-(y-phenylp'ropyl) -N'- ethylure'a' is obtained. 250 g. of this urea derivative are treated with 500 ml. of acetic anhydride, '70 m1. of glacial acetic acid and 136 g. of cyanoacetic acid. By the method described in Example 13, 1 ('y phenylpropyl) 3 ethyl- 6 amino 1,2,3,4 tetrahydro 2,4-pyrimidinedione is obtained, which may be purified by dissolvin in ethyl acetate and precipitating with absolute ether. The white crystals melt at 141-143 C. I

EXAMPLE 18 1 -meth.yl- 3-n-propyl-6-amz'no-1 ,2,3,4-tetrahydm-ZA-pyrimidinedione g. of 1-methyl-6-amino-1,2,3,4-tetrahydro- -2,4-pyrimidinedione is ,treatedwith 264 m1. of 9.

aqueous solution. of sodium hydroxide.

Some of the resultant sodium salt precipitates. 480ml. of 95% ethanoland 204 g. of n-propyl iodide are added and the mixture stirred and heated to reflux temperature for 2 hours. 7 The sodium salt dissolves and reacts during that time. On cooling, a small amount of .the unreacted It is removed by filtration and the alcohol. and unreacted propyl iodide are evaporated on the steam On' cooling, the 1-methyl-3-propyl6- amino 1,2,3,4-tetrahydro 2,4 pyrimidinedione precipitates. It is recrystallized from hot ,water containing a small amount of sodium hydroxide to remove any remaining starting material. Heating at 80 C. for 12 hours yields anhydrous crystals melting at 160-161 C.

: 1 ethyl-3-n prom l-fi-amino-1,2,3,4-tetrahydro- 2,4-pyrimidinedione 100 g. or '1 ethyl-6-amino-1,2,3,4-tetrahydro- We claim: 1. New chemical compounds of the structure 1: HzN-CG \2(J=O H05 3N--R' 0 II o wherein the substituent radicals R and R represent a member of the group consisting of allq l, cycloalkyl and aralkyl radicals and wherein one of these substituent radicals R and R contains a minimum of three carbon atoms.

2. A 1,3 dialkyl 6 amino 1,2,3,4 tetrahydro-2,4-pyrimidinedine of the structure allkyl wherein one of the substituent R groups is an alkyl radical and the other an aralkyl radical.

6. A 1 cycloalkyl 3 alkyl 6 amino 1,2,3,4-tetrahydro-2,4-pyrimidinedione.

10 7. A 1 cyclohexyl 3 alkyl 6 amino 1,2,3,4-tetrahydro-2,4-pyrimidinedione.

8. A 1,3 diaralkyl 6- amino 1,2,3,4 -tetrahydro-ZA-pyrimidinedione of the structure alralkyl /N HzN-G 5 N-araikyl 9. A 1 aralkyl 3 benzyl 6 amino 1,2,3,4 tetrahydro-2,4-pyrimidinedione.

10. 1,3 dibenzyl 6 amino 1,2,3,4 tetrahydro-2,4-pyrimidinedione.

11. A 1,3 dialkyl 6 amino 1,2,3,4 tetrahydro-2,4-pyrimidmedioue of the structure /N mN-c c=o Hal 1L3 wherein one R is a propyl radical and the other R is an ethyl radical.

VIKTOR PAPESCH. ELMER F. SCHROEDER.

REFERENCES CITED The following references are of record in the file of this patent:

Beilstein: Handbuch der Organischen Chemie, vol. 24, p. 472.

Claims (1)

1. A NEW CHEMICAL COMPOUNDS OF THE STRUCTURE